Dissertations / Theses on the topic 'Carbon-Based Nanomaterials -Biomedical Application'

Trimetallic nitride endohedral fullerenes (TNT-EMF) have been recognized for their multifunctional capabilities in biomedical applications. Functionalized gadolinium-loaded fullerenes attracted much attention as a potential new nanoplatform for next-generation magnetic resonance imaging (MRI) contrast agents, given their inherent higher 1H relaxivity than most commercial contrast agents. The fullerene cage is an extraordinarily stable species which makes it extremely unlikely to break and release the toxic Gd metal ions into the bioenvironment. In addition, radiolabeled metals could be encapsulated in this robust carbon cage to deliver therapeutic irradiation. In this dissertation, we aim to develop a series of functionalized TNT-EMFs for MRI detection of various pathological conditions, such as brain cancer, chronic osteomyelitis, and gastrointestinal (GI) tract. As a general introduction, Chapter 1 briefly introduces recent progress in developing metallofullerenes for next-generation biomedical applications. Of special interest are MRI contrast agents. Other potential biomedical applications, toxicity, stability and biodistribution of metallofullerenes are also discussed. Finally, the challenges and future outlook of using fullerene in biomedical and diagnosis applications are summarized at the end of this chapter. The large carbon surface area is ideally suited for multiple exo-functionalization approaches to modify the hydrophobic fullerene cage for a more hydrophilic bio-environment. Additionally, peptides and other agents are readily covalently attached to this nanoprobe for targeting applications. Chapter 2 presents the functionalized metallofullerenes conjugated with interleukin-13 peptide exhibits enhanced targeting of U-251 glioblastoma multiforme (GBM) cell lines and can be effectively delivered intravenously in an orthotopic GBM mouse model. Chapter 3 shows, with the specific targeting moiety, the functionalized metallofullerenes can be applied as a non-invasive imaging approach to detect and differentiate chronic post-traumatic osteomyelitis from aseptic inflammation. Fullerene is a powerful antioxidant due to delocalization of the π-electrons over the carbon cage, which can readily react with free radicals and subsequently delivers a cascade of downstream possessions in numerous biomedical applications. Chapter 4 investigates the antioxidative and anti-inflammatory properties of functionalized Gd3N@C80. This nanoplatform would hold great promise as a novel class of theranostic agent in combating oxidative stress and resolving inflammation, given their inherent MRI applications. In chapter 5, Gd3N@C80 is modified with polyethylene glycol (PEG) for working as MRI contrast agents for GI tract. The high molecular weight can prevent any appreciable absorption through the skin or mucosal tissue, and offer considerable advantages for localized agents in the GI tract. Besides the excellent contrast capability, the PEGylated-Gd3N@C80 exhibits outstanding radical scavenging ability, which can potentially eliminate the reactive oxygen species in GI tract. The biodistribution result suggests this nanoplatform can be worked as the potential contrast agent for GI tract at least for 6 hours. A novel amphiphilic Gd3N@C80 derivative is discussed in Chapter 6. It has been noticed for a long time the functionalization Gd3N@C80 contrast agents have higher relaxivity at lower concentrations. The explanation for the concentration dependency is not fully understood. In this work, the amphiphilic Gd3N@C80 derivative is used as the model to investigate the relationship between the relaxivity and concentration of the Gd-based fullerenes. Click chemistry has been extensively used in functionalization due to the high efficiency and technical simplicity of the reaction. Appendix A describes a new type of Sc3N@C80 derivative conducted by employing the click reaction. The structure of Sc3N@C80-alkynyl and Sc3N@C80- alkynyl-benzyl azide are characterized by NMR, MALDI-TOF, UV-Vis, and HPLC. The high yield of the click reaction can provide access to various derivatives which have great potential for application in medical and materials science. The functionalization and characterizations of Ho3N@C80 derivatives are reported in Appendix B. The contrast ability of Ho3N@C80 is directly compared with Gd3N@C80. The Ho-based fullerenes can be performed as the radiotherapeutic agents; the leaching study is performed to test the stability of carbon cage after irradiation. Appendix C briefly shows a new method to develop Gd3N@C80 based targeting platform, which can be used as the probe for chronic post-traumatic osteomyelitis.
PHD

Carbon nanomaterials have attracted significant attention in the past decades for their unique properties and potential applications in many areas. This dissertation addresses the preparation, functionalization and potential biomedical applications of various carbonaceous nanomaterials. Trimetallic nitride template endohedral metallofullerenes (TNT-EMFs, M₃N@C₈₀, M = Gd, Lu, etc.) are some of the most promising materials for biomedical applications. Water-soluble Gd₃N@C₈₀ was prepared by the functionalization with poly(ethylene glycol) (PEG) and hydroxyl groups (Gd₃N@C₈₀[DiPEG(OH)ₓ]). The length of the PEG chain was tuned by changing the molecular weight of the PEG from 350 to 5000. The 1H magnetic resonance relaxivities of the materials were studied at 0.35 T, 2.4 T and 9.4 T. Their relaxivities were found to increase as the molecular weight of the PEG decreased, which is attributed to the increasing aggregate size. The aggregate sizes were confirmed by dynamic light scattering. In vivo study suggested that Gd3N@C₈₀[DiPEG(OH)x] was a good candidate for magnetic resonance imaging (MRI) contrast agents. Another facile method was also developed to functinalize Gd₃N@C₈₀ with both carboxyl and hydroxyl groups by reaction with succinic acyl peroxide and sodium hydroxide thereafter. The product was determined to be Gd₃N@C₈₀(OH)~₂₆(CH₂CH₂COOM)~₁₆ (M = Na, H) by X-ray photoelectron spectrometry. The Gd₃N@C₈₀(OH)~₂₆(CH₂CH₂COOM)~₁₆ also exhibited high relaxivity, and aggregates in water. The research on both pegylated and carboxylated Gd₃N@C₈₀ suggests that aggregation and rotational correlation time plays an important role in relaxation, and the relaxivities and aggregation of the water-soluble metallofullerenes can be tuned by varying the molecular weight of the functionality. TNT-EMFs can be encapsulated inside single-walled carbon nanotubes (SWNTs) to form "peapod" structures by heating the mixture of TNT-EMFs and SWNTs in a vacuum. The peapods were characterized by Raman spectrometry and transmission electron microscopy (TEM). The peapods were then functionalized with hydroxyl groups by a high speed vibration milling (HSVM) method in the presence of KOH. The functionalized Gd-doped peapods exhibited high relaxivites and had an additional advantage of "double carbon wall" protection of the toxic Gd atoms from possible leaking. The HSVM method was modified by using succinic acyl peroxide. The modified HSVM method could functionalize multi-walled carbon nanotubes (MWNT) and single-walled carbon nanohorns (SWNHs) with carboxyl groups. In the presence of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), carboxylate MWNTs and SWNHs could be conjugated with CdSe/ZnS quantum dots (QDs). TNT-EMFs were also encapsulated inside SWNHs to form SWNH peapods. SWNH peapods were functionalized by the modified HSVM method and then were conjugated with CdSe/ZnS QDs. The peapods were characterized by TEM. In vitro and in vivo studies indicated that SWNH peapods could serve as a multimodal diagnostic agent: MRI contrast agent (Gd₃N@C₈₀ encapsulated), radio-active therapeutic agent (Lu₃N@C₈₀ encapsulated) and optical imaging agent (QDs).
Ph. D.

New synthetic advances in the control of nanoparticle size and shape along with the development of new surface modifications facilitates the growing use of nanomaterials in biomedical applications. Of particular interest are functional and biocompatible nanomaterials for sensing, imaging, and drug delivery. The goal of this research is to tailor the function of nanomaterials for biomedical applications by improving the biocompatibility of the systems. Our work demonstrates both a bottom up and a post synthetic approach for incorporating stability, stealth, and biocompatibility to metal based nanoparticle systems. Two main nanomaterial projects are the focus of this dissertation. We first investigated the development of a green synthetic procedure to produce gold nanoparticles for biological imaging and sensing. The size and morphology of gold nanoparticles directly impact their optical properties, which are important for their function as imaging agents or their use in sensor systems. In this project, a synthetic route based on the natural process of biomineralization was developed, where a designed protein scaffold initiates the nucleation and subsequent growth of gold ions. To gain insight into controlling the size and morphology of the synthesized nanoparticles, interactions between the gold ions and the protein surface were studied along with the effect of ionic strength on interactions and then subsequent crystal growth. We are able to control the size and morphology of the gold nanoparticles by altering the concentration or identity of protein scaffold, salt, or reducing agent. The second project involves the design and optimization of metal organic framework nanoparticles for an external stimulus triggered drug delivery system. This work demonstrates the advantages of using surface coatings for improved stability and functionalization. We show that the addition of a polyethylene glycol surface coating improved the colloidal stability and biocompatibility of the system. The nanoparticle was shown to successfully encapsulate a variety of small molecule cargo. This is the first report of photo-triggered degradation and subsequent release of the loaded cargo as a mechanism of stimuli-controlled drug delivery. Each of the aforementioned projects demonstrates the design, synthesis, and optimization of metal-based systems for use in biomedical applications.
Ph. D.

The evolution of technology has reached a stage where the performances and dimension needed are outpacing what conventional materials can deliver. This has been made more acute with the further necessity of miniaturisation. Therefore, new materials which can overcome this bottleneck are required. Over the past few decades, it was found that when a material is reduced to the nanoscale, they can exhibit properties unparallel by their bulk counterparts. Therefore these nanomaterials poise as a promising candidate for future applications. Of the many nanomaterials, carbon nanotube (CNT) is among the most emblematic. CNT is a hollow one-dimensional structure comprising solely of carbon atoms. They are fascinating as they exhibit physical attributes which surpass many conventional materials and their nanoscale dimension allows greater flexibility in their deployments. However, the utilisation of CNTs is currently frustrated by a host of intrinsic and extrinsic factors. As a result, there are usually significant disparity between their predicted capability and real-world performance. Therefore, the practical application of CNTs remains unfeasible. The premise of this thesis is that by employing CNTs in conjunction with other materials, the hurdles which plague their utilisation may be overcome. Here, the concept of CNT-based hybrid nanomaterials is presented. This thesis demonstrates that by engineering complementary interaction between two materials, many challenges which hamper the utilisations of CNTs and other nanomaterials can indeed be negated. Furthermore, their synergistic interaction allows the performance of the CNT-based hybrid nanomaterials to be superior to their uncoupled precursors. Therefore, this could be a viable strategy to incorporating nanomaterials in a range of applications.

Les travaux de cette thèse ont été consacrés à la synthèse de nanoparticules magnétiques d'oxyde de fer et d'oxyde de cobalt et de nanoparticules coeur-coquille constituées d'un coeur d'oxyde de fer recouvert d'oxyde de cobalt et à l'élaboration de nanomatériaux - composites nanostructures carbonées/nanoparticules d'oxyde métallique - pour des applications dans le domaine biomédical et celui de l'énergie. Pour la synthèse des NPs, la forme et la taille des NPs sont fortement dépendantes des conditions de réaction (nature des ligands, des solvants, température de réaction ... ) , ce qui affecte leurs propriétés magnétiques. De plus, des simulations ont montré que les chaînes de stéarate peuvent désorber plus facilement les atomes de fer que les atomes de cobalt et se libérer pour former des germes, ce qui pourrait expliquer le comportement distinctif entre les deux complexes. Ces nanoparticules magnétiques ont été synthétisées à l'intérieur de nanotubes de carbone en deux étapes aboutissant à des taux de remplissage très importants. Après fonctionnalisation, ces nanocomposites ont été introduits dans de cellules tumorales et ont été magnétiquement manipulées. Ils se sont révélés être très efficaces en tant qu'agents de contraste en IRM mais également dans le domaine de l'hyperthermie (activation sous éclairage dans le domaine de !'Infrarouge proche). Enfin, de nouveaux composites à partir de nanoparticules de Nb20 5 et de graphène (ou NTCs) ont été synthétisés et des résultat~prometteurs ont été obtenus dans des tests de batterie lithium-ion : leur utilisation en tant qu'anode a permis d'obtenir des capacités réversibles de 260 mAh/g
This thesis was focused on the synthesis of magnetic nanoparticles of iron oxide and cobalt oxide and core-shell nanoparticles, consisting of a cobalt oxide coated iron oxide and on the development of composite nanomaterials - nanostructures carbon /metal oxide nanoparticles - for applications in the biomedical field and the energy. For the synthesis of NPs, the shape and size of NPs are dependent of the reaction conditions, which further affect their magnetic properties. Meanwhile, simulation showed that stearate chains can desorb more easily from iron atoms and release to form seeds than from cobalt atoms, which might explain distinctive behavior between the bath complexes. Regarding nanostructures carbon/metal oxide nanoparticles hybrid materials, the properties of the filled magnetic CNTs as heat mediator for photothermal ablation and as contrast agent for MRI were then evaluated and promising results have been obtained. Last, new composite materials (Nb205 nanoparticles/graphene or NTCs) were synthesized and promising results were obtaines in lithium battery tests : their use as anode allowed obtaining reversible capacities of 260 mAh/g

The work developed in this thesis is based on the study of systems for the generation of nanoparticles in liquids by means of pulsed lasers, improving their production and using the generated nanoparticles in applications of great technological relevance such as biomedical imaging or additive manufacturing. For the femtosecond laser production improvement, the novel implementation of a spatio temporal focusing system is proposed which, by varying the temporal pulse duration out-of-focus, suppresses nonlinear effects in the liquid medium and the associated energy losses. For the colloidal size reduction by fragmentation, a continuous flow system is proposed that allows to increase the control over the irradiation parameters while ensuring homogeneous irradiation. Finally, the carbon quantum dots generated are used as fluorescent markers and different metal and oxide nanoparticles are synthesized for their later application as bactericides and in the improvement of materials used in additive manufacturing.
El trabajo desarrollado en esta tesis se basa en el estudio de sistemas de generación de nanopartículas en líquidos mediante láser pulsado, mejorando su producción y empleando los nanomateriales generados en aplicaciones como imagen biomédica o additive manufacturing. Para la mejora en la producción mediante láser de femtosegundo se propone la implementación de un sistema de focalización espacio-temporal que, mediante la variación de la duración temporal de los pulsos fuera de foco, consigue suprimir los efectos no lineales en el medio líquido. Para la mejora en el proceso de reducción del tamaño de coloides, se propone un sistema de flujo continuo que aumenta el control sobre los parámetros de irradiación. Finalmente, los puntos cuánticos de carbono generados se utilizan como marcadores fluorescentes y se sintetizan distintas nanopartículas metálicas y óxidos para su posterior aplicación como bactericidas y en la mejora de materiales utilizados en fabricación aditiva.

Yes
In 1985, the serendipitous discovery of fullerene triggered the research of carbon structures into the world of symmetric nanomaterials. Consequently, Robert F. Curl, Harold W. Kroto and Richard E. Smalley were awarded the Noble prize in chemistry for their discovery of the buckminsterfullerene (C60 with a cage-like fused-ring structure). Fullerene, as the first symmetric nanostructure in carbon nanomaterials family, opened up new perspectives in nanomaterials field leading to discovery and research on other symmetric carbon nanomaterials like carbon nanotubes and two-dimensional graphene which put fullerenes in the shade, while fullerene as the most symmetrical molecule in the world with incredible properties deserves more attention in nanomaterials studies. Buckyball with its unique structure consisting of sp2 carbons which form a high symmetric cage with different sizes (C60, C70 and so on); however, the most abundant among them is C60 which possesses 60 carbon atoms. The combination of unique properties of this molecule extends its applications in divergent areas of science, especially those related to biomedical engineering. This review aims to be a comprehensive review with a broad interest to the biomedical engineering community, being a substantial overview of the most recent advances on fullerenes in biomedical applications that have not been exhaustively and critically reviewed in the past few years.

New derivatives of carbon nanostructures: nanotubes, nano-onions and nanocrystalline diamonds were obtained through fluorination and subsequent functionalization with sucrose. Chemically modified nanocarbons show high solubility in water, ethanol, DMF and can be used as biomaterials for medical applications. It was demonstrated that sucrose functionalized nanostructures can find applications in nanocomposites due to improved dispersion enabled by polyol functional groups. Additionally, pristine and chemically derivatized carbon nanotubes were studied as nanofillers in epoxy composites. Carbon nanotubes tailored with amino functionalities demonstrated better dispersion and crosslinking with epoxy polymer yielding improved tensile strength and elastic properties of nanocomposites.

碩士
國立臺灣海洋大學
生命科學暨生物科技學系
106
Heparin is the most commonly used commercial anticoagulant; however, it can potentially cause very serious side effects. Structural modification of heparin or development of heparin analogs are common methods used to reduce its side effects. These methods often involve complex synthesis procedures and expensive chemicals. Studies suggest that extracts of marine algae are rich in bioactive chemicals with many properties including anticancer, antitumor and antivirus. Sulfated algal polysaccharides, in particular, are known to have anticoagulation properties. However, extraction of such polysaccharides are time consuming and expensive, which bring about the need for chemically sulfating polysaccharides with sulfur compounds. Alginate, a non-sulfated polysaccharide, is a popular choice due to its structural similarity to heparin and low cost. Unfortunately, its low bio-stability and less than satisfactory anticoagulation efficiency limits its application. In this research, we hope to improve the anticoagulation efficiency of alginate by nanonizing its structure and sulfating its functional groups. We successfully synthesized sulfated alginate carbon nanowires (CNWsAlg@SOx) by heat treatment of alginate with ammonium sulfate at 165 °C. We have demonstrated that the CNWsAlg@SOx can inhibit thrombin activity through electrostatic interaction. Thrombin clotting time (TCT) assay revealed our CNWsAlg@SOx has 100-fold longer TCT compared to its precursor. Its low hemolytic activity demonstrated its potential for intravenous administration. Tail bleeding assay revealed that our material is 6-fold more efficient in vivo compared to its alginate precursor. We will extend our research to synthesize carbon nanomaterials by varying the carbon source and sulfur compounds to develop more efficient anticoagulants.

New derivatives of carbon nanostructures: nanotubes, nano-onions and nanocrystalline diamonds were obtained through fluorination and subsequent functionalization with sucrose. Chemically modified nanocarbons show high solubility in water, ethanol, DMF and can be used as biomaterials for medical applications. It was demonstrated that sucrose functionalized nanostructures can find applications in nanocomposites due to improved dispersion enabled by polyol functional groups. Additionally, pristine and chemically derivatized carbon nanotubes were studied as nanofillers in epoxy composites. Carbon nanotubes tailored with amino functionalities demonstrated better dispersion and crosslinking with epoxy polymer yielding improved tensile strength and elastic properties of nanocomposites. Reductive functionalization of nanocarbons, also known as Billups reaction, is a powerful method to yield nanomaterials with high degree of surface functionalization. In this method, nanocarbon salts prepared by treatment with lithium or sodium in liquid ammonia react readily with alkyl and aryl halides as well as bromo carboxylic acids. Functionalized materials are soluble in various organic or aqueous solvents. Water soluble nanodiamond derivatives were also synthesized by reductive functionalization of annealed nanodiamonds. Nanodiamond heat pretreatment was necessary to yield surface graphene layers and facilitate electron transfer from reducing agent to the surface of nanoparticles. Other carbon materials such as activated carbon and anthracite coal were also derivatized using reductive functionalization to yield water soluble activated carbon and partially soluble in organic solvents anthracite. It was shown that activated carbon can be effectively functionalized by Billups method. New derivatives of activated carbon can improve water treatment targeting specific impurities and bio active contaminants. It was demonstrated that functionalized carbon nanotubes are suitable for real time radiation measurements. Radiation sensor incorporating derivatized carbon nanotubes is lightweight and reusable. In summary, functionalization of carbon nanomaterials opens new avenues for processing and applications ranging from biomedicine to radiation sensing in space.

博士
國立清華大學
工程與系統科學系
105
Recently, the issue of energy crisis were raised the attention of which looking for developing new, clean, efficient, and sustainable resources of renewable energy, as well as new technologies associated with energy conversion and storage. For this end, green energy applications for energy generation (DSSCs) and energy storage devices (Supercapacitors) have been promising candidates for the energy requirement. In the first part of this thesis, we developed the directly synthesis of carbon nanotubes (CNTs) on FTO glass at low temperature via the low pressure chemical vapor deposition (LPCVD) method. The specimens were further underwent an electrochemical deposition process to decorate layed-MoS2 nano-catalyst and construct a 3D hybrid nanostructure as counter electrode (C.E.) materials for DSSCs. The DSSC assembled with MoS2/CNTs C.E. exhibiting the photoconversion efficiency value of 7.83 %, which was 9.5 % higher than that of the Pt film. Our findings demonstrated that the MoS2/CNTs hybrid nanostructure is a promising candidate for application as a highly efficient and low-cost C.E. material in Pt-free DSSCs. In the second part, we fabricated the full-carbon hybrid nanoarchitecture of carbon nanofibers/3D graphene (CNFs/3DG), this directly growth of binder-free CNFs/3DG hybrid nanoarchitecture provides strong adhesion to the substrate, low internal resistance, and excellently vertical and horizontal electron transmission ability for electron collection for supercapacitors application. In the third part, we provide an economic strategy of facile transition process of carbon nanomaterials surface from hydrophobic to hydrophilic by Ethanol-treatment process. Moreover, the CV-acid treatment further improve the ELDC by actived meso-/micro-pore structure at the electrode/electrolyte interface and introduced the pseudocapacitance by decorated surface oxygen-containing groups. This method remarkably enhanced the capacitance, energy density, and could be a promising candidate in high-performance supercapacitor applications.

Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008.
Source: Dissertation Abstracts International, Volume: 69-11, Section: B, page: 6638. Adviser: Yi Lu. Includes bibliographical references. Available on microfilm from Pro Quest Information and Learning.

Mestrado em Engenharia Química
Nanoscience has recently experienced a strong development, Magnetic Nanoparticles (MNPs) are one of the most attractive nanomaterials. Focusing on the biomedical applications, this thesis has as main objective the development of new carbon coating methods in order to reach the maximum biocompatibility of MNPs upon synthesis. During the research carried out, two different approaches were evaluated to coat a magnetic core composed of magnetite, using phloroglucinol and glyoxal, following the idea of making the process more sustainable and biocompatible. The difference between those approaches resides on the use of PF-127 as porogen agent during the coating step. However, some significant differences were found for the material synthesized without PF-127 as porogen agent, with the most important one being the lack of stabilization in water, a crucial characteristic of MNPs for biomedical applications. This mishap leaded to the continuation of the methodology development with just one material. The material selected was evaluated as nanocarrier to load and deliver drugs using doxorubicin (DOX) and omeprazole (OME). The delivery was tested at different pH values in order to evaluate its influence, as human body has different pH in a normal tissue (pH 7.4) than in the intracellular tumor environment (pH 4.5) or in its surroundings (pH 6.0).
A nanociência tem experimentado recentemente um forte desenvolvimento. As nanopartículas magnéticas (MNPs) têm sido um dos materiais mais atraentes. Com foco nas aplicações biomédicas, esta tese tem como objetivo principal desenvolver novos métodos de revestimento de carbono para alcançar a máxima biocompatibilidade durante a síntese de MNPs. Durante a pesquisa serão avaliadas duas abordagens diferentes para revestir um núcleo magnético feito de magnetita, as duas utilizan floroglucinol e glioxal, seguindo a ideia de tornar o processo mais sustentável e biocompatível. A diferença entre essas abordagens será sobre o emprego da PF-127 como agente porógeno durante a etapa de revestimento. No entanto, algumas diferenças significativas foram encontradas que o material sintetizado sem a PF-127 como agente porógeno não estava arquivando uma das características mais importantes das MNPs para aplicações biomédicas, a estabilização na água. Este mishap conduziu a continuar a metodologia apenas com um material. O material selecionado foi avaliado para carga e entrega de medicamentos com doxorrubicina e omeprazol. A entrega foi testada em diferentes valores de pH para avaliar sua influência, pois o corpo humano tem pH diferente em um tecido normal (pH 7,4) do que no ambiente tumoral intracelular (pH 4,5) ou em seu entorno (pH 6,0).

碩士
國立中興大學
化學系所
101
A novel silver nanocluster modified screen-printed carbon electrode (SPCE) was prepared for iodide determination. This silver nanocluster synthesized by chemical reduction based on the nanosized templates (~50 nm) of Au@carbon nanomaterials was drop-coated at SPCE to prepare the Ag@Au/Carbon modified electrode (designed as Ag@AuCNPs-SPCE). The SEM images and EDS analysis of Ag@AuCNPs-SPCE show acicular-leaf liked silver nanoclusters formed discretely on carbon nanomaterials. More interestingly, high selective detection of 100 μM I− at Ag@AuCNPs-SPCE in the presence of 500 μM Br−, 6 mM Cl− and 10 fold concentration of (NO－, CH3COO－, SCN－, IO3－, CO32－, C2O42－, CrO42－, Br－) was observed compared to a commercial Ag disk electrode. A detection limit (S/N = 3) of 5 μM and a wide linear working range up to 10 mM in pH 7, 0.1 M PBS was obtained. Good reproducibility and repeatability（RSD<3%） was obtained for iodide detection. In addition, we interested in the antibacterial activity of Ag@carbon nanomaterials (designed as Ag@CNPs) for Gram negative bacteria (E. coli). The antibacterial activities test was carried out by the diameter of inhibition zone (DIZ) in a disk diffusion test. A clear and significantly DIZ (100% larger than that of CNPs) for E. coli was observed after 16 h of incubation, indicating limited bacterial toxicity.