Journal articles on the topic 'Canopy chlorophyll content (CCC)'

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1

Ali, Abebe Mohammed, Roshanak Darvishzadeh, Andrew Skidmore, Marco Heurich, Marc Paganini, Uta Heiden, and Sander Mücher. "Evaluating Prediction Models for Mapping Canopy Chlorophyll Content Across Biomes." Remote Sensing 12, no. 11 (June 1, 2020): 1788. http://dx.doi.org/10.3390/rs12111788.

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Accurate measurement of canopy chlorophyll content (CCC) is essential for the understanding of terrestrial ecosystem dynamics through monitoring and evaluating properties such as carbon and water flux, productivity, light use efficiency as well as nutritional and environmental stresses. Information on the amount and distribution of CCC helps to assess and report biodiversity indicators related to ecosystem processes and functional aspects. Therefore, measuring CCC continuously and globally from earth observation data is critical to monitor the status of the biosphere. However, generic and robust methods for regional and global mapping of CCC are not well defined. This study aimed at examining the spatiotemporal consistency and scalability of selected methods for CCC mapping across biomes. Four methods (i.e., radiative transfer models (RTMs) inversion using a look-up table (LUT), the biophysical processor approach integrated into the Sentinel application platform (SNAP toolbox), simple ratio vegetation index (SRVI), and partial least square regression (PLSR)) were evaluated. Similarities and differences among CCC products generated by applying the four methods on actual Sentinel-2 data in four biomes (temperate forest, tropical forest, wetland, and Arctic tundra) were examined by computing statistical measures and spatiotemporal consistency pairwise comparisons. Pairwise comparison of CCC predictions by the selected methods demonstrated strong agreement. The highest correlation (R2 = 0.93, RMSE = 0.4371 g/m2) was obtained between CCC predictions of PROSAIL inversion by LUT and SNAP toolbox approach in a wetland when a single Sentinel-2 image was used. However, when time-series data were used, it was PROSAIL inversion against SRVI (R2 = 0.88, RMSE = 0.19) that showed greatest similarity to the single date predictions (R2 = 0.83, RMSE = 0.17 g/m2) in this biome. Generally, the CCC products obtained using the SNAP toolbox approach resulted in a systematic over/under-estimation of CCC. RTMs inversion by LUT (INFORM and PROSAIL) resulted in a non-biased, spatiotemporally consistent prediction of CCC with a range closer to expectations. Therefore, the RTM inversion using LUT approaches particularly, INFORM for ‘forest’ and PROSAIL for ‘short vegetation’ ecosystems, are recommended for CCC mapping from Sentinel-2 data for worldwide mapping of CCC. Additional validation of the two RTMs with field data of CCC across biomes is required in the future.
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2

Sun, Qi, Quanjun Jiao, Xiaojin Qian, Liangyun Liu, Xinjie Liu, and Huayang Dai. "Improving the Retrieval of Crop Canopy Chlorophyll Content Using Vegetation Index Combinations." Remote Sensing 13, no. 3 (January 29, 2021): 470. http://dx.doi.org/10.3390/rs13030470.

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Estimates of crop canopy chlorophyll content (CCC) can be used to monitor vegetation productivity, manage crop resources, and control disease and pests. However, making these estimates using conventional ground-based methods is time-consuming and resource-intensive when deployed over large areas. Although vegetation indices (VIs), derived from satellite sensor data, have been used to estimate CCC, they suffer from problems related to spectral saturation, soil background, and canopy structure. A new method was, therefore, proposed for combining the Medium Resolution Imaging Spectrometer (MERIS) terrestrial chlorophyll index (MTCI) and LAI-related vegetation indices (LAI-VIs) to increase the accuracy of CCC estimates for wheat and soybeans. The PROSAIL-D canopy reflectance model was used to simulate canopy spectra that were resampled to match the spectral response functions of the MERIS carried on the ENVISAT satellite. Combinations of the MTCI and LAI-VIs were then used to estimate CCC via univariate linear regression, binary linear regression and random forest regression. The accuracy using the field spectra and MERIS data was determined based on field CCC measurements. All the MTCI and LAI-VI combinations for the selected regression techniques resulted in more accurate estimates of CCC than the use of the MTCI alone (field spectra data for soybeans and wheat: R2 = 0.62 and RMSE = 77.10 μg cm−2; MERIS satellite data for soybeans: R2 = 0.24 and RMSE = 136.54 μg cm−2). The random forest regression resulted in better accuracy than the other two linear regression models. The combination resulting in the best accuracy was the MTCI and MTVI2 and random forest regression, with R2 = 0.65 and RMSE = 37.76 μg cm−2 (field spectra data) and R2 = 0.78 and RMSE = 47.96 μg cm−2 (MERIS satellite data). Combining the MTCI and a LAI-VI represents a further step towards improving the accuracy of estimation CCC based on multispectral satellite sensor data.
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3

Hoeppner, J. Malin, Andrew K. Skidmore, Roshanak Darvishzadeh, Marco Heurich, Hsing-Chung Chang, and Tawanda W. Gara. "Mapping Canopy Chlorophyll Content in a Temperate Forest Using Airborne Hyperspectral Data." Remote Sensing 12, no. 21 (October 31, 2020): 3573. http://dx.doi.org/10.3390/rs12213573.

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Chlorophyll content, as the primary pigment driving photosynthesis, is directly affected by many natural and anthropogenic disturbances and stressors. Accurate and timely estimation of canopy chlorophyll content (CCC) is essential for effective ecosystem monitoring to allow for successful management interventions to occur. Hyperspectral remote sensing offers the possibility to accurately estimate and map canopy chlorophyll content. In the past, research has predominantly focused on the use of hyperspectral data on canopy chlorophyll content retrieval of crops and grassland ecosystems. Therefore, in this study, a temperate mixed forest, the Bavarian Forest National Park in Germany, was chosen as the study site. We compared different statistical models (narrowband vegetation indices (VIs), partial least squares regression (PLSR) and random forest (RF)) in their accuracy to predict CCC using airborne hyperspectral data. The airborne hyperspectral imagery was acquired by the AisaFenix sensor (623 bands; 3.5 nm spectral resolution in the visible near-infrared (VNIR) region, and 12 nm spectral resolution in the shortwave infrared (SWIR) region; 3 m spatial resolution) on July 6, 2017. In situ leaf chlorophyll content and leaf area index measurements were sampled from the upper canopy of coniferous, mixed, and deciduous forest stands in July and August 2017. The study yielded the highest retrieval accuracies with PLSR (root mean square error (RMSE) = 0.25 g/m2, R2 = 0.66). It further indicated specific spectral regions within the visible (390–400 nm and 470–540 nm), red edge (680–780 nm), near-infrared (1050–1100 nm) and shortwave infrared regions (2000–2270 nm) that were important for CCC retrieval. The results showed that forest CCC can be mapped with relatively high accuracies using image spectroscopy.
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4

Bai, Xueyuan, Yingqiang Song, Ruiyang Yu, Jingling Xiong, Yufeng Peng, Yuanmao Jiang, Guijun Yang, Zhenhai Li, and Xicun Zhu. "Hyperspectral Estimation of Apple Canopy Chlorophyll Content Using an Ensemble Learning Approach." Applied Engineering in Agriculture 37, no. 3 (2021): 505–11. http://dx.doi.org/10.13031/aea.13935.

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HighlightsMonitored the canopy chlorophyll content of apple trees using hyperspectral reflectance information.Constructed support vector machine combination regression model (C-SVR) based on five-fold cross validation and support vector machine regression approach.Compared estimation accuracy of ensemble learning models (C-SVR, RF), machine learning models (SVR, ANN), and PLSR models for apple canopy chlorophyll content.Abstract. Rapidly and effective monitoring of the canopy chlorophyll content (CCC) of apple trees is of great significance for crop stress monitoring in precision agriculture. This study attempted to use hyperspectral vegetation indices (VIs) to estimate the CCC of apple trees based on ensemble learning approach. In this study, vegetation indices combined by any two wavelengths from 400 to 1100 nm were constructed to calculate the correlation coefficient with the CCC in apple. We constructed a partial least squares regression model (PLSR), artificial neural network regression model (ANN), support vector machine regression (SVR), random forest regression (RF) model and support vector machine combination regression model (C-SVR) based on combinations of VIs to improve the estimation accuracy in apple CCC. The results showed that the correlation coefficients between NDVI (949,695), OSAVI (828,705), RDVI (741,725), RVI (716,707), DVI (572,532), and apple CCC were all above 0.76. The CCC estimation model using the RF and C-SVR approach constructed by the NDVI (949,695), OSAVI (828,705), RDVI (741,725), RVI (716,707), and DVI (572,532) achieved the better estimation results, and the R2V, RMSEV, and RPDV values of models were 0.76, 0.131(mg . g-1), 2.04 and 0.78, 0.127(mg . g-1), 2.12, respectively. Compared with the PLSR, ANN, and SVR model, the R2V and RPDV values of C-SVR model were increased by 4%, 1.2%, 3.8%, and 5.0%, 28.4%, 7.1%, respectively. The results show that using C-SVR approach to estimating the apple CCC can realize high accuracy of quantitative estimation. Ensemble learning approach is an effective method for monitoring the nutrient status of fruit trees based on hyperspectral technique. Keywords: Apple tree canopy, Chlorophyll content, Crop stress monitoring, Ensemble learning, Hyperspectral, Vegetation index.
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5

Zillmann, E., M. Schönert, H. Lilienthal, B. Siegmann, T. Jarmer, P. Rosso, and T. Weichelt. "Crop Ground Cover Fraction and Canopy Chlorophyll Content Mapping using RapidEye imagery." ISPRS - International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences XL-7/W3 (April 28, 2015): 149–55. http://dx.doi.org/10.5194/isprsarchives-xl-7-w3-149-2015.

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Remote sensing is a suitable tool for estimating the spatial variability of crop canopy characteristics, such as canopy chlorophyll content (CCC) and green ground cover (GGC%), which are often used for crop productivity analysis and site-specific crop management. Empirical relationships exist between different vegetation indices (VI) and CCC and GGC% that allow spatial estimation of canopy characteristics from remote sensing imagery. However, the use of VIs is not suitable for an operational production of CCC and GGC% maps due to the limited transferability of derived empirical relationships to other regions. Thus, the operational value of crop status maps derived from remotely sensed data would be much higher if there was no need for reparametrization of the approach for different situations. <br><br> This paper reports on the suitability of high-resolution RapidEye data for estimating crop development status of winter wheat over the growing season, and demonstrates two different approaches for mapping CCC and GGC%, which do not rely on empirical relationships. The final CCC map represents relative differences in CCC, which can be quickly calibrated to field specific conditions using SPAD chlorophyll meter readings at a few points. The prediction model is capable of predicting SPAD readings with an average accuracy of 77%. The GGC% map provides absolute values at any point in the field. A high R² value of 80% was obtained for the relationship between estimated and observed GGC%. The mean absolute error for each of the two acquisition dates was 5.3% and 8.7%, respectively.
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6

Yang, Hongye, Bo Ming, Chenwei Nie, Beibei Xue, Jiangfeng Xin, Xingli Lu, Jun Xue, et al. "Maize Canopy and Leaf Chlorophyll Content Assessment from Leaf Spectral Reflectance: Estimation and Uncertainty Analysis across Growth Stages and Vertical Distribution." Remote Sensing 14, no. 9 (April 28, 2022): 2115. http://dx.doi.org/10.3390/rs14092115.

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Accurate estimation of the canopy chlorophyll content (CCC) plays a key role in quantitative remote sensing. Maize (Zea mays L.) is a high-stalk crop with a large leaf area and deep canopy. It has a non-uniform vertical distribution of the leaf chlorophyll content (LCC), which limits remote sensing of CCC. Therefore, it is crucial to understand the vertical heterogeneity of LCC and leaf reflectance spectra to improve the accuracy of CCC monitoring. In this study, CCC, LCC, and leaf spectral reflectance were measured during two consecutive field growing seasons under five nitrogen treatments. The vertical LCC profile showed an asymmetric ‘bell-shaped’ curve structure and was affected by nitrogen application. The leaf reflectance also varied greatly between spatio–temporal conditions, which could indicate the influence of vertical heterogeneity. In the early growth stage, the spectral differences between leaf positions were mainly concentrated in the red-edge (RE) and near-infrared (NIR) regions, whereas differences were concentrated in the visible region during the mid-late filling stage. LCC had a strong linear correlation with vegetation indices (VIs), such as the modified red-edge ratio (mRER, R2 = 0.87), but the VI–chlorophyll models showed significant inversion errors throughout the growth season, especially at the early vegetative growth stage and the late filling stage (rRMSE values ranged from 36% to 87.4%). The vertical distribution of LCC had a strong correlation with the total chlorophyll in canopy, and sensitive leaf positions were identified with a multiple stepwise regression (MSR) model. The LCC of leaf positions L6 in the vegetative stage (R2-adj = 0.9) and L11 + L14 in the reproductive stage (R2-adj = 0.93) could be used to evaluate the canopy chlorophyll status (L12 represents the ear leaf). With a strong relationship between leaf spectral reflectance and LCC, CCC can be estimated directly by leaf spectral reflectance (mRER, rRMSE = 8.97%). Therefore, the spatio–temporal variations of LCC and leaf spectral reflectance were analyzed, and a higher accuracy CCC estimation approach that can avoid the effects of the leaf area was proposed.
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7

Jiao, Quanjun, Qi Sun, Bing Zhang, Wenjiang Huang, Huichun Ye, Zhaoming Zhang, Xiao Zhang, and Binxiang Qian. "A Random Forest Algorithm for Retrieving Canopy Chlorophyll Content of Wheat and Soybean Trained with PROSAIL Simulations Using Adjusted Average Leaf Angle." Remote Sensing 14, no. 1 (December 25, 2021): 98. http://dx.doi.org/10.3390/rs14010098.

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Canopy chlorophyll content (CCC) is an important indicator for crop-growth monitoring and crop productivity estimation. The hybrid method, involving the PROSAIL radiative transfer model and machine learning algorithms, has been widely applied for crop CCC retrieval. However, PROSAIL’s homogeneous canopy hypothesis limits the ability to use the PROSAIL-based CCC estimation across different crops with a row structure. In addition to leaf area index (LAI), average leaf angle (ALA) is the most important canopy structure factor in the PROSAIL model. Under the same LAI, adjustment of the ALA can make a PROSAIL simulation obtain the same canopy gap as the heterogeneous canopy at a specific observation angle. Therefore, parameterization of an adjusted ALA (ALAadj) is an optimal choice to make the PROSAIL model suitable for specific row-planted crops. This paper attempted to improve PROSAIL-based CCC retrieval for different crops, using a random forest algorithm, by introducing the prior knowledge of crop-specific ALAadj. Based on the field reflectance spectrum at nadir, leaf area index, and leaf chlorophyll content, parameterization of the ALAadj in the PROSAIL model for wheat and soybean was carried out. An algorithm integrating the random forest and PROSAIL simulations with prior ALAadj information was developed for wheat and soybean CCC retrieval. Ground-measured CCC measurements were used to validate the CCC retrieved from canopy spectra. The results showed that the ALAadj values (62 degrees for wheat; 45 degrees for soybean) that were parameterized for the PROSAIL model demonstrated good discrimination between the two crops. The proposed algorithm improved the CCC retrieval accuracy for wheat and soybean, regardless of whether continuous visible to near-infrared spectra with 50 bands (RMSE from 39.9 to 32.9 μg cm−2; R2 from 0.67 to 0.76) or discrete spectra with 13 bands (RMSE from 43.9 to 33.7 μg cm−2; R2 from 0.63 to 0.74) and nine bands (RMSE from 45.1 to 37.0 μg cm−2; R2 from 0.61 to 0.71) were used. The proposed hybrid algorithm, based on PROSAIL simulations with ALAadj, has the potential for satellite-based CCC estimation across different crop types, and it also has a good reference value for the retrieval of other crop parameters.
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8

Červená, L., G. Pinlová, Z. Lhotáková, E. Neuwirthová, L. Kupková, M. Potůčková, J. Lysák, P. Campbell, and J. Albrechtová. "DETERMINATION OF CHLOROPHYLL CONTENT IN SELECTED GRASS COMMUNITIES OF KRKONOŠE MTS. TUNDRA BASED ON LABORATORY SPECTROSCOPY AND AERIAL HYPERSPECTRAL DATA." International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences XLIII-B3-2022 (May 30, 2022): 381–88. http://dx.doi.org/10.5194/isprs-archives-xliii-b3-2022-381-2022.

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Abstract. The study focuses on the determination of chlorophyll content in four prevailing grasses in the relict arctic-alpine tundra located in the Krkonoše Mountains National Park, Czech Republic. We compared two methods for determination of leaf chlorophyll content (LCC) – spectrophotometric determination in the laboratory, and the LCC assessed by fluorescence portable chlorophyll meter CCM-300. Relationships were established between the LCCs and vegetation indices calculated from leaf spectra acquired with contact probe coupled with an ASD FieldSpec4 Wide-Res spectroradiometer. Canopy chlorophyll contents (CCC) were computed from the LCCs and green leaf area index (LAI), and modelled based on the field spectra measured by the spectroradiometer and the hyperspectral images acquired by Headwall Nano-Hyperspec® mounted on the DJI Matrice 600 Pro drone. The calculations are performed on datasets acquired in June, July and August 2020 together and separately for species and months. In general, the correlations based on June datasets work the best at both levels: median R2 for all indices was 0.52 for all species together at leaf level and median R2 = 0.47 at the canopy level (vegetation indices computed from field spectra). Canopy chlorophyll content map was created based on the results of stepwise multiple linear regression. The R2 was 0.42 when using four wavelengths from the red and red edge spectral region. We attribute the weak model performance to a combination of several factors: leaf structure may bias LCC from laboratory measurements, effects of LAI variability on CCC, and the sampling design, probably not covering the whole phenology equally for all studied species.
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Kamenova, Ilina, Petar Dimitrov, and Rusina Yordanova. "Evaluation of RapidEye vegetation indices for prediction of biophysical/biochemical variables of winter wheat." Aerospace Research in Bulgaria 30 (2018): 63–74. http://dx.doi.org/10.3897/arb.v30.e06.

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The aim of the study is to evaluate the possibility for using RapidEye data for prediction of Leaf Area Index (LAI), fraction of Absorbed Photosynthetically Active Radiation (fAPAR), fraction of vegetation Cover (fCover), leaf Chlorophyll Concentration (CC) and Canopy Chlorophyll Content (CCC) of winter wheat. The relation of a number of vegetation indices (VIs) with these crop variables are accessed based on a regression analysis. Indices, which make use of the red edge band, such as Chlorophyll Index red edge (CIre) and red edge Normalized Difference Vegetation Index (reNDVI), were found most useful, resulting in linear models with R2 of 0.67, 0.71, 0.72, and 0.76 for fCover, LAI, CCC, and fAPAR respectively. CC was not related with any of the VIs.
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10

Brown, Luke A., Booker O. Ogutu, and Jadunandan Dash. "Estimating Forest Leaf Area Index and Canopy Chlorophyll Content with Sentinel-2: An Evaluation of Two Hybrid Retrieval Algorithms." Remote Sensing 11, no. 15 (July 25, 2019): 1752. http://dx.doi.org/10.3390/rs11151752.

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Estimates of biophysical and biochemical variables such as leaf area index (LAI) and canopy chlorophyll content (CCC) are a fundamental requirement for effectively monitoring and managing forest environments. With its red-edge bands and high spatial resolution, the Multispectral Instrument (MSI) on board the Sentinel-2 missions is particularly well-suited to LAI and CCC retrieval. Using field data collected throughout the growing season at a deciduous broadleaf forest site in Southern England, we evaluated the performance of two hybrid retrieval algorithms for estimating LAI and CCC from MSI data: the Scattering by Arbitrarily Inclined Leaves (SAIL)-based L2B retrieval algorithm made available to users in the Sentinel Application Platform (SNAP), and an alternative retrieval algorithm optimised for forest environments, trained using the Invertible Forest Reflectance Model (INFORM). Moderate performance was associated with the SNAP L2B retrieval algorithm for both LAI (r2 = 0.54, RMSE = 1.55, NRMSE = 43%) and CCC (r2 = 0.52, RMSE = 0.79 g m−2, NRMSE = 45%), while improvements were obtained using the INFORM-based retrieval algorithm, particularly in the case of LAI (r2 = 0.79, RMSE = 0.47, NRMSE = 13%), but also in the case of CCC (r2 = 0.69, RMSE = 0.52 g m−2, NRMSE = 29%). Forward modelling experiments confirmed INFORM was better able to reproduce observed MSI spectra than SAIL. Based on our results, for forest-related applications using MSI data, we recommend users seek retrieval algorithms optimised for forest environments.
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11

Candiani, Gabriele, Giulia Tagliabue, Cinzia Panigada, Jochem Verrelst, Valentina Picchi, Juan Pablo Rivera Caicedo, and Mirco Boschetti. "Evaluation of Hybrid Models to Estimate Chlorophyll and Nitrogen Content of Maize Crops in the Framework of the Future CHIME Mission." Remote Sensing 14, no. 8 (April 8, 2022): 1792. http://dx.doi.org/10.3390/rs14081792.

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In the next few years, the new Copernicus Hyperspectral Imaging Mission (CHIME) is foreseen to be launched by the European Space Agency (ESA). This mission will provide an unprecedented amount of hyperspectral data, enabling new research possibilities within several fields of natural resources, including the “agriculture and food security” domain. In order to efficiently exploit this upcoming hyperspectral data stream, new processing methods and techniques need to be studied and implemented. In this work, the hybrid approach (HYB) and its variant, featuring sampling dimensionality reduction through active learning heuristics (HAL), were applied to CHIME-like data to evaluate the retrieval of crop traits, such as chlorophyll and nitrogen content at both leaf (LCC and LNC) and canopy level (CCC and CNC). The results showed that HYB was able to provide reliable estimations at canopy level (R2 = 0.79, RMSE = 0.38 g m−2 for CCC and R2 = 0.84, RMSE = 1.10 g m−2 for CNC) but failed at leaf level. The HAL approach improved retrieval accuracy at canopy level (best metric: R2 = 0.88 and RMSE = 0.21 g m−2 for CCC; R2 = 0.93 and RMSE = 0.71 g m−2 for CNC), providing good results also at leaf level (best metrics: R2 = 0.72 and RMSE = 3.31 μg cm−2 for LCC; R2 = 0.56 and RMSE = 0.02 mg cm−2 for LNC). The promising results obtained through the hybrid approach support the feasibility of an operational retrieval of chlorophyll and nitrogen content, e.g., in the framework of the future CHIME mission. However, further efforts are required to investigate the approach across different years, sites and crop types in order to improve its transferability to other contexts.
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Wang, Li, Shuisen Chen, Zhiping Peng, Jichuan Huang, Chongyang Wang, Hao Jiang, Qiong Zheng, and Dan Li. "Phenology Effects on Physically Based Estimation of Paddy Rice Canopy Traits from UAV Hyperspectral Imagery." Remote Sensing 13, no. 9 (May 4, 2021): 1792. http://dx.doi.org/10.3390/rs13091792.

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Radiation transform models such as PROSAIL are widely used for crop canopy reflectance simulation and biophysical parameter inversion. The PROSAIL model basically assumes that the canopy is turbid homogenous media with a bare soil background. However, the canopy structure changes when crop growth stages develop, which is more or less a departure from this assumption. In addition, a paddy rice field is inundated most of the time with flooded soil background. In this study, field-scale paddy rice leaf area index (LAI), leaf cholorphyll content (LCC), and canopy chlorophyll content (CCC) were retrieved from unmanned-aerial-vehicle-based hyperspectral images by the PROSAIL radiation transform model using a lookup table (LUT) strategy, with a special focus on the effects of growth-stage development and soil-background signature selection. Results show that involving flooded soil reflectance as background reflectance for PROSAIL could improve estimation accuracy. When using a LUT with the flooded soil reflectance signature (LUTflooded) the coefficients of determination (R2) between observed and estimation variables are 0.70, 0.11, and 0.79 for LAI, LCC, and CCC, respectively, for the entire growing season (from tillering to heading growth stages), and the corresponding mean absolute errors (MAEs) are 21.87%, 16.27%, and 12.52%. For LAI and LCC, high model bias mainly occurred in tillering growth stages. There is an obvious overestimation of LAI and underestimation of LCC for in the tillering growth stage. The estimation accuracy of CCC is relatively consistent from tillering to heading growth stages.
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De Grave, Charlotte, Luca Pipia, Bastian Siegmann, Pablo Morcillo-Pallarés, Juan Pablo Rivera-Caicedo, José Moreno, and Jochem Verrelst. "Retrieving and Validating Leaf and Canopy Chlorophyll Content at Moderate Resolution: A Multiscale Analysis with the Sentinel-3 OLCI Sensor." Remote Sensing 13, no. 8 (April 7, 2021): 1419. http://dx.doi.org/10.3390/rs13081419.

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ESA’s Eighth Earth Explorer mission “FLuorescence EXplorer” (FLEX) will be dedicated to the global monitoring of the chlorophyll fluorescence emitted by vegetation. In order to properly interpret the measured fluorescence signal, essential vegetation variables need to be retrieved concomitantly. FLEX will fly in tandem formation with Sentinel-3 (S3), which conveys the Ocean and Land Color Instrument (OLCI) that is designed to characterize the atmosphere and the terrestrial vegetation at a spatial resolution of 300 m. In support of FLEX’s preparatory activities, this paper presents a first validation exercise of OLCI vegetation products against in situ data coming from the 2018 FLEXSense campaign. During this campaign, leaf chlorophyll content (LCC) and leaf area index (LAI) measurements were collected over croplands, while HyPlant DUAL images of the area were acquired at a 3 m spatial resolution. A multiscale validation strategy was pursued. First, estimates of these two variables, together with the combined canopy chlorophyll content (CCC = LCC × LAI), were obtained at the HyPlant spatial resolution and were compared against the in situ measurements. Second, the fine-scale retrieval maps from HyPlant were coarsened to the S3 spatial scale as a reference to assess the quality of the OLCI vegetation products. As an intermediary step, vegetation products extracted from Sentinel-2 data were used to compare retrievals at the in-between spatial resolution of 20 m. For all spatial scales, CCC delivered the most accurate estimates with the smallest prediction error obtained at the 300 m resolution (R2 of 0.74 and RMSE = 26.8 μg cm−2). Results of a scaling analysis suggest that CCC performs well at the different tested spatial resolutions since it presents a linear behavior across scales. LCC, on the other hand, was poorly retrieved at the 300 m scale, showing overestimated values over heterogeneous pixels. The introduction of a new LCC model integrating mixed reflectance spectra in its training enabled to improve by 16% the retrieval accuracy for this variable (RMSE = 10 μg cm−2 for the new model versus RMSE = 11.9 μg cm−2 for the former model).
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Grewal, H. S., and J. S. Kolar. "Response of Brassica juncea to chlorocholine chloride and ethrel sprays in association with nitrogen application." Journal of Agricultural Science 114, no. 1 (January 1990): 87–91. http://dx.doi.org/10.1017/s0021859600071033.

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SUMMARYField studies conducted in 1983/84–1985/86 at Ludhiana showed that the response of physiological and yield characters of Brassica juncea to foliar sprays of chlorocholine chloride as cycocel (CCC) at 250 and 500 p.p.m. and ethrel at 500, 1000 and 1500 p.p.m. differed at different doses of N (O, 50 and 100 kg/ha). The crop did not respond to CCC or ethrel in the absence of N, whereas a significant response was obtained with 250 p.p.m. CCC or 500 p.p.m. ethrel at 50 kg N/ha and 500 p.p.m. CCC or 1000 p.p.m. ethrel at 100 kg N/ha. Response to increasing doses of N increased in the presence of CCC or ethrel spray. The highest concentration of ethrel (1500 p.p.m.) proved detrimental at 0 and N/ha. CCC and ethrel reduced the crop canopy, enhanced the chlorophyll content of leaves, interception of photosynthetically active radiation and sink capacity (number of pods per plant and 1000-seed weight) at 50 and 100 kg N/ha. A higher leaf area index was obtained during the pod development phase with CCC and ethrel sprays. The oil content and germination potential of seeds from crops treated with CCC (250 and 500 p.p.m.) and ethrel (500 and 1000 p.p.m.) were as high as in the untreated crop, irrespective of N dose. However, 1500 p.p.m. of ethrel sprayed on a crop raised without N suppressed germination capacity.
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Chakhvashvili, Erekle, Bastian Siegmann, Onno Muller, Jochem Verrelst, Juliane Bendig, Thorsten Kraska, and Uwe Rascher. "Retrieval of Crop Variables from Proximal Multispectral UAV Image Data Using PROSAIL in Maize Canopy." Remote Sensing 14, no. 5 (March 3, 2022): 1247. http://dx.doi.org/10.3390/rs14051247.

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Mapping crop variables at different growth stages is crucial to inform farmers and plant breeders about the crop status. For mapping purposes, inversion of canopy radiative transfer models (RTMs) is a viable alternative to parametric and non-parametric regression models, which often lack transferability in time and space. Due to the physical nature of RTMs, inversion outputs can be delivered in sound physical units that reflect the underlying processes in the canopy. In this study, we explored the capabilities of the coupled leaf–canopy RTM PROSAIL applied to high-spatial-resolution (0.015 m) multispectral unmanned aerial vehicle (UAV) data to retrieve the leaf chlorophyll content (LCC), leaf area index (LAI) and canopy chlorophyll content (CCC) of sweet and silage maize throughout one growing season. Two different retrieval methods were tested: (i) applying the RTM inversion scheme to mean reflectance data derived from single breeding plots (mean reflectance approach) and (ii) applying the same inversion scheme to an orthomosaic to separately retrieve the target variables for each pixel of the breeding plots (pixel-based approach). For LCC retrieval, soil and shaded pixels were removed by applying simple vegetation index thresholding. Retrieval of LCC from UAV data yielded promising results compared to ground measurements (sweet maize RMSE = 4.92 µg/m2, silage maize RMSE = 3.74 µg/m2) when using the mean reflectance approach. LAI retrieval was more challenging due to the blending of sunlit and shaded pixels present in the UAV data, but worked well at the early developmental stages (sweet maize RMSE = 0.70 m2/m2, silage RMSE = 0.61 m2/m2 across all dates). CCC retrieval significantly benefited from the pixel-based approach compared to the mean reflectance approach (RMSEs decreased from 45.6 to 33.1 µg/m2). We argue that high-resolution UAV imagery is well suited for LCC retrieval, as shadows and background soil can be precisely removed, leaving only green plant pixels for the analysis. As for retrieving LAI, it proved to be challenging for two distinct varieties of maize that were characterized by contrasting canopy geometry.
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Wang, Siheng, Dong Yang, Zhen Li, Liangyun Liu, Changping Huang, and Lifu Zhang. "A Global Sensitivity Analysis of Commonly Used Satellite-Derived Vegetation Indices for Homogeneous Canopies Based on Model Simulation and Random Forest Learning." Remote Sensing 11, no. 21 (October 30, 2019): 2547. http://dx.doi.org/10.3390/rs11212547.

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Remote sensing (RS) provides operational monitoring of terrestrial vegetation. For optical RS, vegetation information is generally derived from surface reflectance (ρ). More generally, vegetation indices (VIs) are built on the basis of ρ as proxies for vegetation traits. At canopy level, ρ can be affected by a variety of factors, including leaf constituents, canopy structure, background reflectivity, and sun-sensor geometry. Consequently, VIs are mixtures of different information. In this study, a global sensitivity analysis (GSA) is made for several commonly used satellite-derived VIs in order to better understand the application of these VIs at large scales. The sensitivities of VIs to different parameters are analyzed on the basis of PROSPECT-SAIL (PROSAIL) radiative transfer model simulations, which apply for homogeneous canopies, and random forest (RF) learning. Specifically, combined factors such as canopy chlorophyll content (CCC) and canopy water content (CWC) are introduced in the RF-based GSA. We find that for most VIs, the leaf area index is the most influential factor, while the broad-band sensor-derived enhanced VI (EVI) exhibits a strong sensitivity to CCC, and the universal normalized VI (UNVI) is sensitive to CWC. The potential and uncertainty for the application of all the considered VIs are analyzed according to the GSA results. The results can help to improve the use of VIs in different contexts, and the RF-based GSA method can be further applied in more sophisticated situations.
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Sakowska, Karolina, Radoslaw Juszczak, and Damiano Gianelle. "Remote Sensing of Grassland Biophysical Parameters in the Context of the Sentinel-2 Satellite Mission." Journal of Sensors 2016 (2016): 1–16. http://dx.doi.org/10.1155/2016/4612809.

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This study investigates the potential of the Sentinel-2 satellite for monitoring the seasonal changes in grassland total canopy chlorophyll content (CCC), fraction of photosynthetically active radiation absorbed by the vegetation canopy (FAPAR), and fraction of photosynthetically active radiation absorbed only by its photosynthesizing components (GFAPAR). Reflectance observations were collected on a continuous basis during growing seasons by means of a newly developed ASD-WhiteRef system. Two models using Sentinel-2 simulated data (linear regression-vegetation indices (VIs) approach and multiple regression (MR) reflectance approach) were tested to estimate vegetation biophysical parameters. To assess whether the use of full solar spectrum reflectance data is able to provide an added value in CCC and GFAPAR estimation accuracy, a third model based on partial least squares regression (PLSR) and the ASD-WhiteRef reflectance data was tested. The results showed that FAPAR remained quite stable during the reproduction and senescence stages, and no significant relationships between FAPAR and VIs were found. On the other hand, GFAPAR showed clearer seasonal trends. The comparison of the three models revealed no significant differences in the accuracies of CCC and GFAPAR predictions and demonstrated a strong contribution of SWIR bands to the explained variability of investigated parameters. The promising results highlight the potential of the Sentinel-2 satellite for retrieving biophysical parameters from space.
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Kganyago, Mahlatse, Clement Adjorlolo, and Paidamwoyo Mhangara. "Exploring Transferable Techniques to Retrieve Crop Biophysical and Biochemical Variables Using Sentinel-2 Data." Remote Sensing 14, no. 16 (August 15, 2022): 3968. http://dx.doi.org/10.3390/rs14163968.

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The current study aimed to determine the spatial transferability of eXtreme Gradient Boosting (XGBoost) models for estimating biophysical and biochemical variables (BVs), using Sentinel-2 data. The specific objectives were to: (1) assess the effect of different proportions of training samples (i.e., 25%, 50%, and 75%) available at the Target site (DT) on the spatial transferability of the XGBoost models and (2) evaluate the effect of the Source site (DS) (i.e., trained) model accuracy on the Target site (i.e., unseen) retrieval uncertainty. The results showed that the Bothaville (DS) → Harrismith (DT) Leaf Area Index (LAI) models required only fewer proportions, i.e., 25% or 50%, of the training samples to make optimal retrievals in the DT (i.e., RMSE: 0.61 m2 m−2; R2: 59%), while Harrismith (DS) →Bothaville (DT) LAI models required up to 75% of training samples in the DT to obtain optimal LAI retrievals (i.e., RMSE = 0.63 m2 m−2; R2 = 67%). In contrast, the chlorophyll content models for Bothaville (DS) → Harrismith (DT) required significant proportions of samples (i.e., 75%) from the DT to make optimal retrievals of Leaf Chlorophyll Content (LCab) (i.e., RMSE: 7.09 µg cm−2; R2: 58%) and Canopy Chlorophyll Content (CCC) (i.e., RMSE: 36.3 µg cm−2; R2: 61%), while Harrismith (DS) →Bothaville (DT) models required only 25% of the samples to achieve RMSEs of 8.16 µg cm−2 (R2: 83%) and 40.25 µg cm−2 (R2: 77%), for LCab and CCC, respectively. The results also showed that the source site model accuracy led to better transferability for LAI retrievals. In contrast, the accuracy of LCab and CCC source site models did not necessarily improve their transferability. Overall, the results elucidate the potential of transferable Machine Learning Regression Algorithms and are significant for the rapid retrieval of important crop BVs in data-scarce areas, thus facilitating spatially-explicit information for site-specific farm management.
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Casella, Alejandra, Luciano Orden, Néstor A. Pezzola, Carolina Bellaccomo, Cristina I. Winschel, Gabriel R. Caballero, Jesús Delegido, Luis Manuel Navas Gracia, and Jochem Verrelst. "Analysis of Biophysical Variables in an Onion Crop (Allium cepa L.) with Nitrogen Fertilization by Sentinel-2 Observations." Agronomy 12, no. 8 (August 11, 2022): 1884. http://dx.doi.org/10.3390/agronomy12081884.

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The production of onions bulbs (Allium cepa L.) requires a high amount of nitrogen. According to the demand of sustainable agriculture, the information-development and communication technologies allow for improving the efficiency of nitrogen fertilization. In the south of the province of Buenos Aires, Argentina, between 8000 and 10,000 hectares per year−1 are cultivated in the districts of Villarino and Patagones. This work aimed to analyze the relationship of biophysical variables: leaf area index (LAI), canopy chlorophyll content (CCC), and canopy cover factor (fCOVER), with the nitrogen fertilization of an intermediate cycle onion crop and its effects on yield. A field trial study with different doses of granulated urea and granulated urea was carried out, where biophysical characteristics were evaluated in the field and in Sentinel-2 satellite observations. Field data correlated well with satellite data, with an R2 of 0.91, 0.96, and 0.85 for LAI, fCOVER, and CCC, respectively. The application of nitrogen in all its doses produced significantly higher yields than the control. The LAI and CCC variables had a positive correlation with yield in the months of November and December. A significant difference was observed between U250 (62 Mg ha−1) and the other treatments. The U500 dose led to a yield increase of 27% compared to U250, while the difference between U750 and U500 was 6%.
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Kganyago, Mahlatse, Paidamwoyo Mhangara, and Clement Adjorlolo. "Estimating Crop Biophysical Parameters Using Machine Learning Algorithms and Sentinel-2 Imagery." Remote Sensing 13, no. 21 (October 27, 2021): 4314. http://dx.doi.org/10.3390/rs13214314.

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Global food security is critical to eliminating hunger and malnutrition. In the changing climate, farmers in developing countries must adopt technologies and farming practices such as precision agriculture (PA). PA-based approaches enable farmers to cope with frequent and intensified droughts and heatwaves, optimising yields, increasing efficiencies, and reducing operational costs. Biophysical parameters such as Leaf Area Index (LAI), Leaf Chlorophyll Content (LCab), and Canopy Chlorophyll Content (CCC) are essential for characterising field-level spatial variability and thus are necessary for enabling variable rate application technologies, precision irrigation, and crop monitoring. Moreover, robust machine learning algorithms offer prospects for improving the estimation of biophysical parameters due to their capability to deal with non-linear data, small samples, and noisy variables. This study compared the predictive performance of sparse Partial Least Squares (sPLS), Random Forest (RF), and Gradient Boosting Machines (GBM) for estimating LAI, LCab, and CCC with Sentinel-2 imagery in Bothaville, South Africa and identified, using variable importance measures, the most influential bands for estimating crop biophysical parameters. The results showed that RF was superior in estimating all three biophysical parameters, followed by GBM which was better in estimating LAI and CCC, but not LCab, where sPLS was relatively better. Since all biophysical parameters could be achieved with RF, it can be considered a good contender for operationalisation. Overall, the findings in this study are significant for future biophysical product development using RF to reduce reliance on many algorithms for specific parameters, thus facilitating the rapid extraction of actionable information to support PA and crop monitoring activities.
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Djamai, Najib, Detang Zhong, Richard Fernandes, and Fuqun Zhou. "Evaluation of Vegetation Biophysical Variables Time Series Derived from Synthetic Sentinel-2 Images." Remote Sensing 11, no. 13 (June 29, 2019): 1547. http://dx.doi.org/10.3390/rs11131547.

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Time series of vegetation biophysical variables (leaf area index (LAI), fraction canopy cover (FCOVER), fraction of absorbed photosynthetically active radiation (FAPAR), canopy chlorophyll content (CCC), and canopy water content (CWC)) were estimated from interpolated Sentinel-2 (S2-LIKE) surface reflectance images, for an agricultural region located in central Canada, using the Simplified Level 2 Product Prototype Processor (SL2P). S2-LIKE surface reflectance data were generated by blending clear-sky Sentinel-2 Multispectral Imager (S2-MSI) images with daily BRDF-adjusted Moderate Resolution Imaging Spectrometer images using the Prediction Smooth Reflectance Fusion Model (PSFRM), and validated using thirteen independent S2-MSI images (RMSE ≤ 6%). The uncertainty of S2-LIKE surface reflectance data increases with the time delay between the prediction date and the closest S2-MSI image used for training PSFRM. Vegetation biophysical variables from S2-LIKE products are validated qualitatively and quantitatively by comparison to the corresponding vegetation biophysical variables from S2-MSI products (RMSE = 0.55 for LAI, ~10% for FCOVER and FAPAR, and 0.13 g/m2 for CCC and 0.16 kg/m2 for CWC). Uncertainties of vegetation biophysical variables derived from S2-LIKE products are almost linearly related to the uncertainty of the input reflectance data. When compared to the in situ measurements collected during the Soil Moisture Active Passive Validation Experiment 2016 field campaign, uncertainties of LAI (0.83) and FCOVER (13.73%) estimates from S2-LIKE products were slightly larger than uncertainties of LAI (0.57) and FCOVER (11.80%) estimates from S2-MSI products. However, equal uncertainties (0.32 kg/m2) were obtained for CWC estimates using SL2P with either S2-LIKE or S2-MSI input data.
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Revill, Andrew, Anna Florence, Alasdair MacArthur, Stephen Hoad, Robert Rees, and Mathew Williams. "The Value of Sentinel-2 Spectral Bands for the Assessment of Winter Wheat Growth and Development." Remote Sensing 11, no. 17 (August 31, 2019): 2050. http://dx.doi.org/10.3390/rs11172050.

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Leaf Area Index (LAI) and chlorophyll content are strongly related to plant development and productivity. Spatial and temporal estimates of these variables are essential for efficient and precise crop management. The availability of open-access data from the European Space Agency’s (ESA) Sentinel-2 satellite—delivering global coverage with an average 5-day revisit frequency at a spatial resolution of up to 10 metres—could provide estimates of these variables at unprecedented (i.e., sub-field) resolution. Using synthetic data, past research has demonstrated the potential of Sentinel-2 for estimating crop variables. Nonetheless, research involving a robust analysis of the Sentinel-2 bands for supporting agricultural applications is limited. We evaluated the potential of Sentinel-2 data for retrieving winter wheat LAI, leaf chlorophyll content (LCC) and canopy chlorophyll content (CCC). In coordination with destructive and non-destructive ground measurements, we acquired multispectral data from an Unmanned Aerial Vehicle (UAV)-mounted sensor measuring key Sentinel-2 spectral bands (443 to 865 nm). We applied Gaussian processes regression (GPR) machine learning to determine the most informative Sentinel-2 bands for retrieving each of the variables. We further evaluated the GPR model performance when propagating observation uncertainty. When applying the best-performing GPR models without propagating uncertainty, the retrievals had a high agreement with ground measurements—the mean R2 and normalised root-mean-square error (NRMSE) were 0.89 and 8.8%, respectively. When propagating uncertainty, the mean R2 and NRMSE were 0.82 and 11.9%, respectively. When accounting for measurement uncertainty in the estimation of LAI and CCC, the number of most informative Sentinel-2 bands was reduced from four to only two—the red-edge (705 nm) and near-infrared (865 nm) bands. This research demonstrates the value of the Sentinel-2 spectral characteristics for retrieving critical variables that can support more sustainable crop management practices.
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Zhuo, Wei, Nan Wu, Runhe Shi, and Zuo Wang. "UAV Mapping of the Chlorophyll Content in a Tidal Flat Wetland Using a Combination of Spectral and Frequency Indices." Remote Sensing 14, no. 4 (February 10, 2022): 827. http://dx.doi.org/10.3390/rs14040827.

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The chlorophyll content of leaves is an important indicator of plant environmental stress, photosynthetic capacity, and is widely used to diagnose the growth and health status of vegetation. Traditional chlorophyll content inversion is based on the vegetation index under pure species, which rarely considers the impact of interspecific competition and species mixture on the inversion accuracy. To solve these limitations, the harmonic analysis (HA) and the Hilbert–Huang transform (HHT) were introduced to obtain the frequency index, which were combined with spectral index as the input parameters to estimate chlorophyll content based on the unmanned aerial vehicle (UAV) image. The research results indicated that: (1) Based on a comparison of the model accuracy for three different types of indices in the same period, the estimation accuracy of the pure spectral index was the lowest, followed by that of the frequency index, whereas the mixed index estimation effect was the best. (2) The estimation accuracy in November was lower than that in other months; the pure spectral index coefficient of determination (R2) was only 0.5208, and the root–mean–square error (RMSE) was 4.2144. The estimation effect in September was the best. The model R2 under the mixed index reached 0.8283, and the RMSE was 2.0907. (3) The canopy chlorophyll content (CCC) estimation under the frequency domain index was generally better than that of the pure spectral index, indicating that the frequency information was more sensitive to subtle differences in the spectrum of mixed vegetation. These research results show that the combination of spectral and frequency information can effectively improve the mapping accuracy of the chlorophyll content, and provid a theoretical basis and technology for monitoring the chlorophyll content of mixed vegetation in wetlands.
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Abdelbaki, Asmaa, Martin Schlerf, Rebecca Retzlaff, Miriam Machwitz, Jochem Verrelst, and Thomas Udelhoven. "Comparison of Crop Trait Retrieval Strategies Using UAV-Based VNIR Hyperspectral Imaging." Remote Sensing 13, no. 9 (April 30, 2021): 1748. http://dx.doi.org/10.3390/rs13091748.

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Hyperspectral cameras onboard unmanned aerial vehicles (UAVs) have recently emerged for monitoring crop traits at the sub-field scale. Different physical, statistical, and hybrid methods for crop trait retrieval have been developed. However, spectra collected from UAVs can be confounded by various issues, including illumination variation throughout the crop growing season, the effect of which on the retrieval performance is not well understood at present. In this study, four retrieval methods are compared, in terms of retrieving the leaf area index (LAI), fractional vegetation cover (fCover), and canopy chlorophyll content (CCC) of potato plants over an agricultural field for six dates during the growing season. We analyzed: (1) The standard look-up table method (LUTstd), (2) an improved (regularized) LUT method that involves variable correlation (LUTreg), (3) hybrid methods, and (4) random forest regression without (RF) and with (RFexp) the exposure time as an additional explanatory variable. The Soil–Leaf–Canopy (SLC) model was used in association with the LUT-based inversion and hybrid methods, while the statistical modelling methods (RF and RFexp) relied entirely on in situ data. The results revealed that RFexp was the best-performing method, yielding the highest accuracies, in terms of the normalized root mean square error (NRMSE), for LAI (5.36%), fCover (5.87%), and CCC (15.01%). RFexp was able to reduce the effects of illumination variability and cloud shadows. LUTreg outperformed the other two retrieval methods (hybrid methods and LUTstd), with an NRMSE of 9.18% for LAI, 10.46% for fCover, and 12.16% for CCC. Conversely, LUTreg led to lower accuracies than those derived from RF for LAI (5.51%) and for fCover (6.23%), but not for CCC (16.21%). Therefore, the machine learning approaches—in particular, RF—appear to be the most promising retrieval methods for application to UAV-based hyperspectral data.
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Kennedy, Blair E., Douglas J. King, and Jason Duffe. "Comparison of Empirical and Physical Modelling for Estimation of Biochemical and Biophysical Vegetation Properties: Field Scale Analysis across an Arctic Bioclimatic Gradient." Remote Sensing 12, no. 18 (September 19, 2020): 3073. http://dx.doi.org/10.3390/rs12183073.

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To evaluate the potential of multi-angle hyperspectral sensors for monitoring vegetation variables in Arctic environments, empirical and physical modelling using field data was implemented for the retrieval of leaf and canopy chlorophyll content (LCC, CCC) and plant area index (PAI) measured at four sites situated across a bioclimatic gradient in the Western Canadian Arctic. Field reflectance data were acquired with an ASD FieldSpec (305–1075 nm) and used to simulate CHRIS Mode1 spectra (411–997 nm). Multi-angle measurements were taken corresponding to CHRIS view zenith angles (VZA) (−55°, −36°, 0°, +36°, +55°). Empirical modelling compared parametric regression based on vegetation indices (VIs) to non-parametric Gaussian Processes Regression (GPR). In physical modelling, PROSAIL was inverted using numerical optimization and look-up table (LUT) approaches. Cross-validation of the empirical models ranked GPR as best, followed by simple ratio (SR) with optimally selected NIR and red wavelengths, and then ROSAVI using its published wavelengths (mean r2cv = 0.62, 0.58, and 0.54, respectively across all sites, variables, and VZAs). However, the best predictive performance was achieved by SR followed by GPR and ROSAVI (NRMSEcv = 0.12, 0.16, 0.16, respectively). PROSAIL simulated the multi-angle top-of-canopy reflectance well with numerical optimization (r2 = ~0.99, RMSE = 0.004 ± 0.002), but best performing LUT models of LCC, CCC and PAI were poorer than the empirical approaches (mean r2 = 0.48, mean NRMSE = 0.22). PROSAIL performed best at the high Arctic sparsely vegetated site (r2 = 0.57–0.86 for all parameters). Overall, the best performing VZA was −55° for empirical modelling and 0° and ±55° for physical modelling; however, these were not significantly better than the other VZAs. Overall, this study demonstrates that, for Arctic vegetation, nadir narrowband reflectance data used to derive simple empirical VIs with optimally selected bands is a more efficient approach for modelling chlorophyll and PAI than more complex empirical and physical approaches.
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D. M. El-Shikha, E. M. Barnes, T. R. Clarke, D. J. Hunsaker, J. A. Haberland, P. J. Pinter Jr., P. M. Waller, and T. L. Thompson. "Remote Sensing of Cotton Nitrogen Status Using the Canopy Chlorophyll Content Index (CCCI)." Transactions of the ASABE 51, no. 1 (2008): 73–82. http://dx.doi.org/10.13031/2013.24228.

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Caballero, Gabriel, Alejandro Pezzola, Cristina Winschel, Alejandra Casella, Paolo Sanchez Angonova, Juan Pablo Rivera-Caicedo, Katja Berger, Jochem Verrelst, and Jesus Delegido. "Seasonal Mapping of Irrigated Winter Wheat Traits in Argentina with a Hybrid Retrieval Workflow Using Sentinel-2 Imagery." Remote Sensing 14, no. 18 (September 10, 2022): 4531. http://dx.doi.org/10.3390/rs14184531.

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Earth observation offers an unprecedented opportunity to monitor intensively cultivated areas providing key support to assess fertilizer needs and crop water uptake. Routinely, vegetation traits mapping can help farmers to monitor plant development along the crop’s phenological cycle, which is particularly relevant for irrigated agricultural areas. The high spatial and temporal resolution of the Sentinel-2 (S2) multispectral instrument leverages the possibility to estimate leaf area index (LAI), canopy chlorophyll content (CCC), and vegetation water content (VWC) from space. Therefore, our study presents a hybrid retrieval workflow combining a physically-based strategy with a machine learning regression algorithm, i.e., Gaussian processes regression, and an active learning technique to estimate LAI, CCC and VWC of irrigated winter wheat. The established hybrid models of the three traits were validated against in-situ data of a wheat campaign in the Bonaerense valley, South of the Buenos Aires Province, Argentina, in the year 2020. We obtained good to highly accurate validation results with LAI: R2 = 0.92, RMSE = 0.43 m2 m−2, CCC: R2 = 0.80, RMSE = 0.27 g m−2 and VWC: R2 = 0.75, RMSE = 416 g m−2. The retrieval models were also applied to a series of S2 images, producing time series along the seasonal cycle, which reflected the effects of fertilizer and irrigation on crop growth. The associated uncertainties along with the obtained maps underlined the robustness of the hybrid retrieval workflow. We conclude that processing S2 imagery with optimised hybrid models allows accurate space-based crop traits mapping over large irrigated areas and thus can support agricultural management decisions.
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Jin, Hongxiao, Christian Josef Köppl, Benjamin M. C. Fischer, Johanna Rojas-Conejo, Mark S. Johnson, Laura Morillas, Steve W. Lyon, et al. "Drone-Based Hyperspectral and Thermal Imagery for Quantifying Upland Rice Productivity and Water Use Efficiency after Biochar Application." Remote Sensing 13, no. 10 (May 11, 2021): 1866. http://dx.doi.org/10.3390/rs13101866.

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Miniature hyperspectral and thermal cameras onboard lightweight unmanned aerial vehicles (UAV) bring new opportunities for monitoring land surface variables at unprecedented fine spatial resolution with acceptable accuracy. This research applies hyperspectral and thermal imagery from a drone to quantify upland rice productivity and water use efficiency (WUE) after biochar application in Costa Rica. The field flights were conducted over two experimental groups with bamboo biochar (BC1) and sugarcane biochar (BC2) amendments and one control (C) group without biochar application. Rice canopy biophysical variables were estimated by inverting a canopy radiative transfer model on hyperspectral reflectance. Variations in gross primary productivity (GPP) and WUE across treatments were estimated using light-use efficiency and WUE models respectively from the normalized difference vegetation index (NDVI), canopy chlorophyll content (CCC), and evapotranspiration rate. We found that GPP was increased by 41.9 ± 3.4% in BC1 and 17.5 ± 3.4% in BC2 versus C, which may be explained by higher soil moisture after biochar application, and consequently significantly higher WUEs by 40.8 ± 3.5% in BC1 and 13.4 ± 3.5% in BC2 compared to C. This study demonstrated the use of hyperspectral and thermal imagery from a drone to quantify biochar effects on dry cropland by integrating ground measurements and physical models.
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Fitzgerald, Glenn, Daniel Rodriguez, and Garry O’Leary. "Measuring and predicting canopy nitrogen nutrition in wheat using a spectral index—The canopy chlorophyll content index (CCCI)." Field Crops Research 116, no. 3 (April 2010): 318–24. http://dx.doi.org/10.1016/j.fcr.2010.01.010.

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Pasqualotto, Nieves, Guido D’Urso, Salvatore Falanga Bolognesi, Oscar Rosario Belfiore, Shari Van Wittenberghe, Jesús Delegido, Alejandro Pezzola, Cristina Winschel, and José Moreno. "Retrieval of Evapotranspiration from Sentinel-2: Comparison of Vegetation Indices, Semi-Empirical Models and SNAP Biophysical Processor Approach." Agronomy 9, no. 10 (October 22, 2019): 663. http://dx.doi.org/10.3390/agronomy9100663.

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Remote sensing evapotranspiration estimation over agricultural areas is increasingly used for irrigation management during the crop growing cycle. Different methodologies based on remote sensing have emerged for the leaf area index (LAI) and the canopy chlorophyll content (CCC) estimation, essential biophysical parameters for crop evapotranspiration monitoring. Using Sentinel-2 (S2) spectral information, this study performed a comparative analysis of empirical (vegetation indices), semi-empirical (CLAIR model with fixed and calibrated extinction coefficient) and artificial neural network S2 products derived from the Sentinel Application Platform Software (SNAP) biophysical processor (ANN S2 products) approaches for the estimation of LAI and CCC. Four independent in situ collected datasets of LAI and CCC, obtained with standard instruments (LAI-2000, SPAD) and a smartphone application (PocketLAI), were used. The ANN S2 products present good statistics for LAI (R2 > 0.70, root mean square error (RMSE) < 0.86) and CCC (R2 > 0.75, RMSE < 0.68 g/m2) retrievals. The normalized Sentinel-2 LAI index (SeLI) is the index that presents good statistics in each dataset (R2 > 0.71, RMSE < 0.78) and for the CCC, the ratio red-edge chlorophyll index (CIred-edge) (R2 > 0.67, RMSE < 0.62 g/m2). Both indices use bands located in the red-edge zone, highlighting the importance of this region. The LAI CLAIR model with a fixed extinction coefficient value produces a R2 > 0.63 and a RMSE < 1.47 and calibrating this coefficient for each study area only improves the statistics in two areas (RMSE ≈ 0.70). Finally, this study analyzed the influence of the LAI parameter estimated with the different methodologies in the calculation of crop potential evapotranspiration (ETc) with the adapted Penman–Monteith (FAO-56 PM), using a multi-temporal dataset. The results were compared with ETc estimated as the product of the reference evapotranspiration (ETo) and on the crop coefficient (Kc) derived from FAO table values. In the absence of independent reference ET data, the estimated ETc with the LAI in situ values were considered as the proxy of the ground-truth. ETc estimated with the ANN S2 LAI product is the closest to the ETc values calculated with the LAI in situ (R2 > 0.90, RMSE < 0.41 mm/d). Our findings indicate the good validation of ANN S2 LAI and CCC products and their further suitability for the implementation in evapotranspiration retrieval of agricultural areas.
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Pascual-Venteo, Ana B., Enrique Portalés, Katja Berger, Giulia Tagliabue, Jose L. Garcia, Adrián Pérez-Suay, Juan Pablo Rivera-Caicedo, and Jochem Verrelst. "Prototyping Crop Traits Retrieval Models for CHIME: Dimensionality Reduction Strategies Applied to PRISMA Data." Remote Sensing 14, no. 10 (May 19, 2022): 2448. http://dx.doi.org/10.3390/rs14102448.

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In preparation for new-generation imaging spectrometer missions and the accompanying unprecedented inflow of hyperspectral data, optimized models are needed to generate vegetation traits routinely. Hybrid models, combining radiative transfer models with machine learning algorithms, are preferred, however, dealing with spectral collinearity imposes an additional challenge. In this study, we analyzed two spectral dimensionality reduction methods: principal component analysis (PCA) and band ranking (BR), embedded in a hybrid workflow for the retrieval of specific leaf area (SLA), leaf area index (LAI), canopy water content (CWC), canopy chlorophyll content (CCC), the fraction of absorbed photosynthetic active radiation (FAPAR), and fractional vegetation cover (FVC). The SCOPE model was used to simulate training data sets, which were optimized with active learning. Gaussian process regression (GPR) algorithms were trained over the simulations to obtain trait-specific models. The inclusion of PCA and BR with 20 features led to the so-called GPR-20PCA and GPR-20BR models. The 20PCA models encompassed over 99.95% cumulative variance of the full spectral data, while the GPR-20BR models were based on the 20 most sensitive bands. Validation against in situ data obtained moderate to optimal results with normalized root mean squared error (NRMSE) from 13.9% (CWC) to 22.3% (CCC) for GPR-20PCA models, and NRMSE from 19.6% (CWC) to 29.1% (SLA) for GPR-20BR models. Overall, the GPR-20PCA slightly outperformed the GPR-20BR models for all six variables. To demonstrate mapping capabilities, both models were tested on a PRecursore IperSpettrale della Missione Applicativa (PRISMA) scene, spectrally resampled to Copernicus Hyperspectral Imaging Mission for the Environment (CHIME), over an agricultural test site (Jolanda di Savoia, Italy). The two strategies obtained plausible spatial patterns, and consistency between the two models was highest for FVC and LAI (R2=0.91, R2=0.86) and lowest for SLA mapping (R2=0.53). From these findings, we recommend implementing GPR-20PCA models as the most efficient strategy for the retrieval of multiple crop traits from hyperspectral data streams. Hence, this workflow will support and facilitate the preparations of traits retrieval models from the next-generation operational CHIME.
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Brown, Luke A., Fernando Camacho, Vicente García-Santos, Niall Origo, Beatriz Fuster, Harry Morris, Julio Pastor-Guzman, et al. "Fiducial Reference Measurements for Vegetation Bio-Geophysical Variables: An End-to-End Uncertainty Evaluation Framework." Remote Sensing 13, no. 16 (August 12, 2021): 3194. http://dx.doi.org/10.3390/rs13163194.

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With a wide range of satellite-derived vegetation bio-geophysical products now available to users, validation efforts are required to assess their accuracy and fitness for purpose. Substantial progress in the validation of such products has been made over the last two decades, but quantification of the uncertainties associated with in situ reference measurements is rarely performed, and the incorporation of uncertainties within upscaling procedures is cursory at best. Since current validation practices assume that reference data represent the truth, our ability to reliably demonstrate compliance with product uncertainty requirements through conformity testing is limited. The Fiducial Reference Measurements for Vegetation (FRM4VEG) project, initiated by the European Space Agency, is aiming to address this challenge by applying metrological principles to vegetation and surface reflectance product validation. Following FRM principles, and in accordance with the International Standards Organisation’s (ISO) Guide to the Expression of Uncertainty in Measurement (GUM), for the first time, we describe an end-to-end uncertainty evaluation framework for reference data of two key vegetation bio-geophysical variables: the fraction of absorbed photosynthetically active radiation (FAPAR) and canopy chlorophyll content (CCC). The process involves quantifying the uncertainties associated with individual in situ reference measurements and incorporating these uncertainties within the upscaling procedure (as well as those associated with the high-spatial-resolution imagery used for upscaling). The framework was demonstrated in two field campaigns covering agricultural crops (Las Tiesas–Barrax, Spain) and deciduous broadleaf forest (Wytham Woods, UK). Providing high-spatial-resolution reference maps with per-pixel uncertainty estimates, the framework is applicable to a range of other bio-geophysical variables including leaf area index (LAI), the fraction of vegetation cover (FCOVER), and canopy water content (CWC). The proposed procedures will facilitate conformity testing of moderate spatial resolution vegetation bio-geophysical products in future validation exercises.
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Abdelbaki, Asmaa, and Thomas Udelhoven. "A Review of Hybrid Approaches for Quantitative Assessment of Crop Traits Using Optical Remote Sensing: Research Trends and Future Directions." Remote Sensing 14, no. 15 (July 22, 2022): 3515. http://dx.doi.org/10.3390/rs14153515.

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Remote sensing technology allows to provide information about biochemical and biophysical crop traits and monitor their spatiotemporal dynamics of agriculture ecosystems. Among multiple retrieval techniques, hybrid approaches have been found to provide outstanding accuracy, for instance, for the inference of leaf area index (LAI), fractional vegetation cover (fCover), and leaf and canopy chlorophyll content (LCC and CCC). The combination of radiative transfer models (RTMs) and data-driven models creates an advantage in the use of hybrid methods. Through this review paper, we aim to provide state-of-the-art hybrid retrieval schemes and theoretical frameworks. To achieve this, we reviewed and systematically analyzed publications over the past 22 years. We identified two hybrid-based parametric and hybrid-based nonparametric regression models and evaluated their performance for each variable of interest. From the results of our extensive literature survey, most research directions are now moving towards combining RTM and machine learning (ML) methods in a symbiotic manner. In particular, the development of ML will open up new ways to integrate innovative approaches such as integrating shallow or deep neural networks with RTM using remote sensing data to reduce errors in crop trait estimations and improve control of crop growth conditions in very large areas serving precision agriculture applications.
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Upreti, Deepak, Wenjiang Huang, Weiping Kong, Simone Pascucci, Stefano Pignatti, Xianfeng Zhou, Huichun Ye, and Raffaele Casa. "A Comparison of Hybrid Machine Learning Algorithms for the Retrieval of Wheat Biophysical Variables from Sentinel-2." Remote Sensing 11, no. 5 (February 26, 2019): 481. http://dx.doi.org/10.3390/rs11050481.

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This study focuses on the comparison of hybrid methods of estimation of biophysical variables such as leaf area index (LAI), leaf chlorophyll content (LCC), fraction of absorbed photosynthetically active radiation (FAPAR), fraction of vegetation cover (FVC), and canopy chlorophyll content (CCC) from Sentinel-2 satellite data. Different machine learning algorithms were trained with simulated spectra generated by the physically-based radiative transfer model PROSAIL and subsequently applied to Sentinel-2 reflectance spectra. The algorithms were assessed against a standard operational approach, i.e., the European Space Agency (ESA) Sentinel Application Platform (SNAP) toolbox, based on neural networks. Since kernel-based algorithms have a heavy computational cost when trained with large datasets, an active learning (AL) strategy was explored to try to alleviate this issue. Validation was carried out using ground data from two study sites: one in Shunyi (China) and the other in Maccarese (Italy). In general, the performance of the algorithms was consistent for the two study sites, though a different level of accuracy was found between the two sites, possibly due to slightly different ground sampling protocols and the range and variability of the values of the biophysical variables in the two ground datasets. For LAI estimation, the best ground validation results were obtained for both sites using least squares linear regression (LSLR) and partial least squares regression, with the best performances values of R2 of 0.78, rott mean squared error (RMSE) of 0.68 m2 m−2 and a relative RMSE (RRMSE) of 19.48% obtained in the Maccarese site with LSLR. The best results for LCC were obtained using Random Forest Tree Bagger (RFTB) and Bagging Trees (BagT) with the best performances obtained in Maccarese using RFTB (R2 = 0.26, RMSE = 8.88 μg cm−2, RRMSE = 17.43%). Gaussian Process Regression (GPR) was the best algorithm for all variables only in the cross-validation phase, but not in the ground validation, where it ranked as the best only for FVC in Maccarese (R2 = 0.90, RMSE = 0.08, RRMSE = 9.86%). It was found that the AL strategy was more efficient than the random selection of samples for training the GPR algorithm.
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Kravchuk, L. A., A. A. Yanovskiy, and N. M. Bazhenova. "REMOTE EVALUATION OF THE INFLUENCE OF VEGETATION COVER ON THE LAND SURFACE TEMPERATURE IN MAIN GEOTECHNICAL SYSTEMS OF THE LARGE CITY (BY THE EXAMPLE OF MINSK)." Nature Management, no. 1 (August 28, 2022): 71–82. http://dx.doi.org/10.47612/2079-3928-2022-1-71-82.

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The influence of vegetation cover on the Land Surface Temperature (LST) was studied in main types of geotechnical systems (GTS) in Minsk (industrial, municipal, residential multi-apartment and estate, public, road, special territories, etc.). A coupled analysis of a differentiated geographic information system (GIS) and Earth remote sensing dat was used. Vegetation cover was assessed using the Normalized Difference Vegetation Index (NDVI), Leaf Area Index (LAI), Canopy chlorophyll content (CCC), and greening degree (%). The analysis revealed significant negative relationships between LST and vegetation indices when comparing all types of territories in the city (R2 varies within 0.42…0.45). Connections are weaker in the main types of GTS. The coefficients of determination LST with NDVI and the greenery degree in industrial and communal, public, residential multi-apartment and estate GTS are estimated respectively at 0.06 (0.31), 0.13 (0.25), 0.18 (0.27), and 0.28 (0.22). This indicates a more significant effect of technogenic elements on LST. Cartographic analysis of the differences between the average LST values in sections of various GTS and natural ecosystems in the urban area from the average values for the corresponding types of GTS and natural complexes revealed areas of the urban territory with increased or decreased LST values. The differences for the GTS and natural ecosystems vary from –3.0° to +3.0° on the most of the territory of Minsk. However, areas with higher differences are noted in the city. The warmest areas are mainly territories of densely built-up industrial zones, separate areas of residential multi-apartment and public area, located in the historical center and in the new buildings on the outskirts of the city. They are characterized by a high density of buildings, hard surfaces, a low greenery degree and sparse of trees and shrubs.
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Ganeva, Dessislava, Eugenia Roumenina, Petar Dimitrov, Alexander Gikov, Georgi Jelev, Rangel Dragov, Violeta Bozhanova, and Krasimira Taneva. "Phenotypic Traits Estimation and Preliminary Yield Assessment in Different Phenophases of Wheat Breeding Experiment Based on UAV Multispectral Images." Remote Sensing 14, no. 4 (February 20, 2022): 1019. http://dx.doi.org/10.3390/rs14041019.

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The utility of unmanned aerial vehicles (UAV) imagery in retrieving phenotypic data to support plant breeding research has been a topic of increasing interest in recent years. The advantages of image-based phenotyping are related to the high spatial and temporal resolution of the retrieved data and the non-destructive and rapid method of data acquisition. This study trains parametric and nonparametric regression models to retrieve leaf area index (LAI), fraction of absorbed photosynthetically active radiation (fAPAR), fractional vegetation cover (fCover), leaf chlorophyll content (LCC), canopy chlorophyll content (CCC), and grain yield (GY) of winter durum wheat breeding experiment from four-bands UAV images. A ground dataset, collected during two field campaigns and complemented with data from a previous study, is used for model development. The dataset is split at random into two parts, one for training and one for testing the models. The tested parametric models use the vegetation index formula and parametric functions. The tested nonparametric models are partial least square regression (PLSR), random forest regression (RFR), support vector regression (SVR), kernel ridge regression (KRR), and Gaussian processes regression (GPR). The retrieved biophysical variables along with traditional phenotypic traits (plant height, yield, and tillering) are analysed for detection of genetic diversity, proximity, and similarity in the studied genotypes. Analysis of variance (ANOVA), Duncan’s multiple range test, correlation analysis, and principal component analysis (PCA) are performed with the phenotypic traits. The parametric and nonparametric models show close results for GY retrieval, with parametric models indicating slightly higher accuracy (R2 = 0.49; RMSE = 0.58 kg/plot; rRMSE = 6.1%). However, the nonparametric model, GPR, computes per pixel uncertainty estimation, making it more appealing for operational use. Furthermore, our results demonstrate that grain filling was better than flowering phenological stage to predict GY. The nonparametric models show better results for biophysical variables retrieval, with GPR presenting the highest prediction performance. Nonetheless, robust models are found only for LAI (R2 = 0.48; RMSE = 0.64; rRMSE = 13.5%) and LCC (R2 = 0.49; RMSE = 31.57 mg m−2; rRMSE = 6.4%) and therefore these are the only remotely sensed phenotypic traits included in the statistical analysis for preliminary assessment of wheat productivity. The results from ANOVA and PCA illustrate that the retrieved remotely sensed phenotypic traits are a valuable addition to the traditional phenotypic traits for plant breeding studies. We believe that these preliminary results could speed up crop improvement programs; however, stronger interdisciplinary research is still needed, as well as uncertainty estimation of the remotely sensed phenotypic traits.
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Chen, Jinghua, Shaoqiang Wang, Bin Chen, Yue Li, Muhammad Amir, Li Ma, Kai Zhu, et al. "Comparative Analysis on the Estimation of Diurnal Solar-Induced Chlorophyll Fluorescence Dynamics for a Subtropical Evergreen Coniferous Forest." Remote Sensing 13, no. 16 (August 9, 2021): 3143. http://dx.doi.org/10.3390/rs13163143.

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Solar-induced chlorophyll fluorescence (SIF) is considered as a prospective indicator of vegetation photosynthetic activity and the ecosystem carbon cycle. The current coarse spatial-temporal resolutions of SIF data from satellite missions and ground measurements still cannot satisfy the corroboration of its correlation with photosynthesis and carbon flux. Practical approaches are needed to be explored for the supplementation of the SIF measurements. In our study, we clarified the diurnal variations of leaf and canopy chlorophyll fluorescence for a subtropical evergreen coniferous forest and evaluated the performance of the canopy chlorophyll concentration (CCC) approach and the backward approach from gross primary production (GPP) for estimating the diurnal variations of canopy SIF by comparing with the Soil Canopy Observation Photosynthesis Energy (SCOPE) model. The results showed that the canopy SIF had similar seasonal and diurnal variations with the incident photosynthetically active radiation (PAR) above the canopy, while the leaf steady-state fluorescence remained stable during the daytime. Neither the CCC nor the raw backward approach from GPP could capture the short temporal dynamics of canopy SIF. However, after improving the backward approach with a correction factor of normalized PAR incident on leaves, the variation of the estimated canopy SIF accounted for more than half of the diurnal variations in the canopy SIF (SIF687: R2 = 0.53, p < 0.001; SIF760: R2 = 0.72, p < 0.001) for the subtropical evergreen coniferous forest without water stress. Drought interfered with the utilization of the improved backward approach because of the decoupling of SIF and GPP due to stomatal closure. This new approach offers new insight into the estimation of diurnal canopy SIF and can help understand the photosynthesis of vegetation for future climate change studies.
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ZHANG, Weiwei, Feng XU, Hua CHENG, Linling LI, Fuliang CAO, and Shuiyuan CHENG. "Effect of Chlorocholine Chloride on Chlorophyll, Photosynthesis, Soluble Sugar and Flavonoids of Ginkgo biloba." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 41, no. 1 (May 28, 2013): 97. http://dx.doi.org/10.15835/nbha4118294.

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The flavonoids content determines the quality characteristics of Ginkgo biloba extract that could be increased by using of plant growth regulators. The objective of study was to investigate the effect of chlorocholine chloride (CCC), an anti-gibberellin growth retardant, on photosynthesis, chlorophyll, soluble sugar, total amino acids and phenylalanine contents, flavonoid accumulation, and flavonoids enzyme activity in G. biloba leaves. The ginkgo seedlings were grown in the greenhouse conditions with foliar applications of 0 (control), 0.5, 1.0 and 2.0 g l-1 CCC. Results showed that 0.5, 1.0 and 2.0 g l-1 CCC treatments significantly increased photosynthetic rates of leaves, the contents of chlorophyll, soluble sugar, total amino acids and phenylalnine in ginkgo leaves. Total polyphenols, flavonoids, anthocyanins content, phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS) and chalcone isomerase (CHI) activities were all significantly increased by 1.0 and 2.0 g l-1 CCC treatments. Foliar treatment with CCC therefore might be a useful means of improving pharmacological properties of G. biloba leaves.
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Yang, Xingchen, Shaogang Lei, Yunxi Shi, and Weizhong Wang. "Effects of Ground Subsidence on Vegetation Chlorophyll Content in Semi-Arid Mining Area: From Leaf Scale to Canopy Scale." International Journal of Environmental Research and Public Health 20, no. 1 (December 28, 2022): 493. http://dx.doi.org/10.3390/ijerph20010493.

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Ground subsidence is the main cause of vegetation degradation in mining areas. It is of great significance to study the effects of ground subsidence on vegetation. At present, few studies have analyzed the effects of ground subsidence on vegetation from different scales. However, the conclusions on different scales may differ. In this experiment, chlorophyll content was used as an indicator of vegetation degradation. We conducted a long-term field survey in the Lijiahao coalfield in China. Based on field survey data and remote sensing images, we analyzed the effects of ground subsidence on chlorophyll content from two scales (leaf scale and canopy scale) and summarized the similarities and differences. We found that, regardless of leaf scale or canopy scale, the effects of subsidence on chlorophyll content have the following three characteristics: (1) mining had the least effect on chlorophyll content in the neutral area, followed by the compression area, and the greatest effect on chlorophyll content in the extension area; (2) subsidence had a slight effect on chlorophyll content of Caragana korshins, but a serious effect on chlorophyll content of Stipa baicalensis; (3) chlorophyll content was not immediately affected when the ground sank. It was the cumulative subsidence that affects chlorophyll content. The difference between leaf scale and canopy scale was that the chlorophyll content at canopy scale is more affected by mining. This means that when assessing vegetation degradation, the results obtained by remote sensing were more severe than those measured in the field. We believe that this is because the canopy chlorophyll content obtained by remote sensing is also affected by the plant canopy structure. We recommend that mining and ecological restoration should be carried out concurrently, and that ground fissures should be taken as the focus of ecological restoration. In addition, Caragana korshins ought to be widely planted. Most importantly, managers should assess the effects of ground subsidence on vegetation on different scales. However, managers need to be aware of differences at different scales.
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Cammarano, Davide, Glenn Fitzgerald, Bruno Basso, Garry O'Leary, Deli Chen, Peter Grace, and Costanza Fiorentino. "Use of the Canopy Chlorophyl Content Index (CCCI) for Remote Estimation of Wheat Nitrogen Content in Rainfed Environments." Agronomy Journal 103, no. 6 (November 2011): 1597–603. http://dx.doi.org/10.2134/agronj2011.0124.

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Qiao, Lang, Dehua Gao, Junyi Zhang, Minzan Li, Hong Sun, and Junyong Ma. "Dynamic Influence Elimination and Chlorophyll Content Diagnosis of Maize Using UAV Spectral Imagery." Remote Sensing 12, no. 16 (August 17, 2020): 2650. http://dx.doi.org/10.3390/rs12162650.

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In order to improve the diagnosis accuracy of chlorophyll content in maize canopy, the remote sensing image of maize canopy with multiple growth stages was acquired by using an unmanned aerial vehicle (UAV) equipped with a spectral camera. The dynamic influencing factors of the canopy multispectral images of maize were removed by using different image segmentation methods. The chlorophyll content of maize in the field was diagnosed. The crop canopy spectral reflectance, coverage, and texture information are combined to discuss the different segmentation methods. A full-grown maize canopy chlorophyll content diagnostic model was created on the basis of the different segmentation methods. Results showed that different segmentation methods have variations in the extraction of maize canopy parameters. The wavelet segmentation method demonstrated better advantages than threshold and ExG index segmentation methods. This method segments the soil background, reduces the texture complexity of the image, and achieves satisfactory results. The maize canopy multispectral band reflectance and vegetation index were extracted on the basis of the different segmentation methods. A partial least square regression algorithm was used to construct a full-grown maize canopy chlorophyll content diagnostic model. The result showed that the model accuracy was low when the image background was not removed (Rc2 (the determination coefficient of calibration set) = 0.5431, RMSEF (the root mean squared error of forecast) = 4.2184, MAE (the mean absolute error) = 3.24; Rv2 (the determination coefficient of validation set) = 0.5894, RMSEP (the root mean squared error of prediction) = 4.6947, and MAE = 3.36). The diagnostic accuracy of the chlorophyll content could be improved by extracting the maize canopy through the segmentation method, which was based on the wavelet segmentation method. The maize canopy chlorophyll content diagnostic model had the highest accuracy (Rc2 = 0.6638, RMSEF = 3.6211, MAE = 2.89; Rv2 = 0.6923, RMSEP = 3.9067, and MAE = 3.19). The research can provide a feasible method for crop growth and nutrition monitoring on the basis of the UAV platform and has a guiding significance for crop cultivation management.
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POURMOHAMMAD, Azam, Mahmoud TOORCHI, Seyed S. ALAVIKIA, and Mohammad R. SHAKIBA. "Genetic Analysis of Yield and Physiological Traits in Sunflower (Helianthus annuus L.) under Irrigation and Drought Stress." Notulae Scientia Biologicae 6, no. 2 (June 10, 2014): 207–13. http://dx.doi.org/10.15835/nsb629173.

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Implementing appropriate breeding strategies for sunflower, alongside dependable information on heritability and gene effects upon yield and related traits under drought conditions, are all necessary. Thirty sunflower hybrids were produced by line × tester cross of six male-sterile and five restorer lines. Their hybrids were evaluated in three levels of irrigation, as follows: (1) non-stressed plots, irrigated at regular intervals (W1); (2) mild water stress (W2), irrigated from the beginning of the button stage (R4) to seed filling initiation (R6); (3) severe water stress (W3) started from the beginning of button stage (R4) to physiological maturity. Based on observations and specific methods for determination, canopy temperatures, chlorophyll index, relative water content and proline content, were studied by additive effects, under the different irrigation conditions. Canopy temperatures,chlorophyll index, relative water content, leaf water potential, proline content and yield were controlled by additive effects under mild stressed conditions. Under severe stress conditions however, canopy temperatures, leaf water potential and proline content were controlled by additive effects, while chlorophyll index and relative water content were controlled by both additive and dominant effects, as seed yield was mainly influenced by the dominant effects. The narrow sense heritability ranged from 47-97% for all traits, except for chlorophyll fluorescence. Yield correlated positively with chlorophyll index and relative water content, and negatively with canopy temperature and leaf water potential. Therefore, under drought stressed conditions in breeding programs, canopy temperatures, chlorophyll index and relative water content can be reliable criteria for the selection of tolerant genotypes with prospect to higher yields.
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Su, Sun, Chen, Zhang, Yao, Wu, Huang, and Zhu. "Joint Retrieval of Growing Season Corn Canopy LAI and Leaf Chlorophyll Content by Fusing Sentinel-2 and MODIS Images." Remote Sensing 11, no. 20 (October 17, 2019): 2409. http://dx.doi.org/10.3390/rs11202409.

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Continuous and accurate estimates of crop canopy leaf area index (LAI) and chlorophyll content are of great importance for crop growth monitoring. These estimates can be useful for precision agricultural management and agricultural planning. Our objectives were to investigate the joint retrieval of corn canopy LAI and chlorophyll content using filtered reflectances from Sentinel-2 and MODIS data acquired during the corn growing season, which, being generally hot and rainy, results in few cloud-free Sentinel-2 images. In addition, the retrieved time series of LAI and chlorophyll content results were used to monitor the corn growth behavior in the study area. Our results showed that: (1) the joint retrieval of LAI and chlorophyll content using the proposed joint probability distribution method improved the estimation accuracy of both corn canopy LAI and chlorophyll content. Corn canopy LAI and chlorophyll content were retrieved jointly and accurately using the PROSAIL model with fused Kalman filtered (KF) reflectance images. The relation between retrieved and field measured LAI and chlorophyll content of four corn-growing stages had a coefficient of determination (R2) of about 0.6, and root mean square errors (RMSEs) ranges of mainly 0.1–0.2 and 0.0–0.3, respectively. (2) Kalman filtering is a good way to produce continuous high-resolution reflectance images by synthesizing Sentinel-2 and MODIS reflectances. The correlation between fused KF and Sentinel-2 reflectances had an R2 value of 0.98 and RMSE of 0.0133, and the correlation between KF and field-measured reflectances had an R2 value of 0.8598 and RMSE of 0.0404. (3) The derived continuous KF reflectances captured the crop behavior well. Our analysis showed that the LAI increased from day of year (DOY) 181 (trefoil stage) to DOY 236 (filling stage), and then increased continuously until harvest, while the chlorophyll content first also increased from DOY 181 to DOY 236, and then remained stable until harvest. These results revealed that the jointly retrieved continuous LAI and chlorophyll content could be used to monitor corn growth conditions.
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Mridha, Nilimesh, Rabi N. Sahoo, Vinay K. Sehgal, Gopal Krishna, Sourabh Pargal, Sanatan Pradhan, Vinod K. Gupta, and Dasika Nagesh Kumar. "Comparative Evaluation of Inversion Approaches of the Radiative Transfer Model for Estimation of Crop Biophysical Parameters." International Agrophysics 29, no. 2 (April 1, 2015): 201–12. http://dx.doi.org/10.1515/intag-2015-0019.

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Abstract The inversion of canopy reflectance models is widely used for the retrieval of vegetation properties from remote sensing. This study evaluates the retrieval of soybean biophysical variables of leaf area index, leaf chlorophyll content, canopy chlorophyll content, and equivalent leaf water thickness from proximal reflectance data integrated broadbands corresponding to moderate resolution imaging spectroradiometer, thematic mapper, and linear imaging self scanning sensors through inversion of the canopy radiative transfer model, PROSAIL. Three different inversion approaches namely the look-up table, genetic algorithm, and artificial neural network were used and performances were evaluated. Application of the genetic algorithm for crop parameter retrieval is a new attempt among the variety of optimization problems in remote sensing which have been successfully demonstrated in the present study. Its performance was as good as that of the look-up table approach and the artificial neural network was a poor performer. The general order of estimation accuracy for parameters irrespective of inversion approaches was leaf area index > canopy chlorophyll content > leaf chlorophyll content > equivalent leaf water thickness. Performance of inversion was comparable for broadband reflectances of all three sensors in the optical region with insignificant differences in estimation accuracy among them.
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Hong, Sun, Li Minzan, Zhang Yane, Zhao Yong, and Wang Haihua. "Detection of Corn Chlorophyll Content Using Canopy Spectral Reflectance." Sensor Letters 8, no. 1 (February 1, 2010): 134–39. http://dx.doi.org/10.1166/sl.2010.1215.

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Morgun, V., G. Pryadkina, O. Stasik, and O. Zborivska. "Relationships between canopy assimilation surface capacity traits and grain productivity of winter wheat genotypes under drought stress." Agricultural Science and Practice 6, no. 2 (July 15, 2019): 18–28. http://dx.doi.org/10.15407/agrisp6.02.018.

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Aim. A comparative analysis of several traits of the capacity of the assimilation apparatus of 10 varieties and 2 lines of winter wheat from Ukraine, under conditions of insuffi cient precipitation and elevated air temperature during the period, when the reproductive organs formed (GS 30–49), in order to search for phenotypic markers associated with high productivity. Methods. Field, morphometric, spectrophotometric and statistical methods were used. Results. The maximum difference in yield between varieties and lines, which grew under condi- tions of insuffi cient water supply and high temperatures in April and May of growing season 2017/2018, was 24.7 %. Under these conditions, the highest grain productivity was observed for the new varieties Pochayna, Hospodarka and Kyivska 17 (8.60–8.73 t/ha) and a high canopy leaves chlorophyll index at late stages of ontogenesis (0.38-0.48 g chlorophyll/m 2 at milky-wax ripeness). This was opposed to varieties Smuhlianka, Poradnytsia and the line UK 392/15 with the lowest yield (7.00–7.25 t/ha) and assimilation surface at this stage (0.07–0.17 g chlorophyll/m 2 ). At the fl owering stage (anthesis) the most productive varieties exceeded the least productive ones, on average, by 30 % in leaves fresh weight of the canopy, by 24 % in content of total (a+b) chlorophyll and by 60 % in canopy chlorophyll index. At milky-wax ripeness, the differences between these varieties increased signifi cantly – up to 136 % in leaf fresh weight of canopy, 57 % in chlorophyll content and 350 % in canopy leaves chlorophyll index. A close positive correlation (r = 0.69–0.77, P ˂ 0.01) between the canopy photosynthetic apparatus traits at milky-wax ripeness with the yield of varieties and lines of winter wheat under drought and high temperature stress was found. Conclusions. The results show that the leaves fresh weight of canopy and canopy leaves chlorophyll index can be used as markers of grain productivity of winter wheat under drought stress, as well as for the possible development of molecular genetic criteria of breeding, based on these phenotypic characteristics.
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Santini, Paula Tristão, Lorena Gabriela Almeida, Kamila Rezende Dázio de Souza, João Paulo Rodrigues Alves Delfino Barbosa, and José Donizeti Alves. "SPATIO-TEMPORAL VARIABILITY OF CARBOHYDRATE AND CHLOROPHYLL CONTENT IN THE COFFEE CANOPY." Coffee Science 14, no. 3 (September 25, 2019): 366. http://dx.doi.org/10.25186/cs.v14i3.1590.

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The spatial variability of the total chlorophyll content and carotenoids content, starch and soluble sugars of coffee canopy were mapped throughout the day. Therefore, evaluations were carried out in a ‘Catuaí Vermelho’ coffee plant with 1.7 meters height. A vertical gradient (from the apex to the base of the plant canopy) and a horizontal gradient (plagiotropic branches) were established to analyze different positions of the canopy. Thus, in the vertical direction, four heights were analyzed in the plant: top, upper, middle and lower regions. In the horizontal gradient, the plagiotropic branches were divided into three parts: basal, median and apical. Collection of leaf samples was performed on the east and west sides of the canopy, at 9 a.m., totaling 24 collection points at each time. Higher content of photosynthetic pigments and concentration of sugars were observed in the western face and in the inner parts of the coffee tree. The content of chloroplast pigments and sugars of an individual coffee leaf diverge considerably from other leaves, which requires caution when scaling estimates at the global canopy level. The analysis of some punctual leaves does not serve to discriminate the overall dynamics of a canopy.
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48

Hakala, T., O. Nevalainen, S. Kaasalainen, and R. Mäkipää. "Technical Note: Multispectral lidar time series of pine canopy chlorophyll content." Biogeosciences 12, no. 5 (March 12, 2015): 1629–34. http://dx.doi.org/10.5194/bg-12-1629-2015.

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Abstract. We present an empirical application of multispectral laser scanning for monitoring the seasonal and spatial changes in pine chlorophyll (a + b) content and upscaling the accurate leaf-level chlorophyll measurements into branch and tree level. The results show the capability of the new instrument for monitoring the changes in the shape and physiology of tree canopy: the spectral indices retrieved from the multispectral point cloud agree with laboratory measurements of the chlorophyll a and b content. The approach opens new prospects for replacing destructive and labour-intensive manual sampling with remote observations of tree physiology.
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49

Öztürk, İ. "Association between physiological parameters and yield in Triticum aestivum L. genotypes under rainfed conditions." Agricultural Science and Technology 12, no. 2 (June 2020): 107–13. http://dx.doi.org/10.15547/ast.2020.02.018.

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Abstract. The purpose of the study was to assess the relationships between physiological parameters and grain yield of different bread wheat genotypes. In the present research a total of 25 bread wheat genotypes were tested during the 2016-2017 seasons under rainfed conditions. The experiment was conducted in a randomized complete blocks design with four replications. Grain yield, days of heading, plant height, biomass (NDVI) from GS25 up to GS85 growth stage, chlorophyll content (SPAD) during the heading stage, canopy temperature (CT) at GS60 and GS75 growth stages, and glaucousness were investigated. The results of variance analyses showed that there were significant differences (p<0.01) among genotypes for yield. The mean grain yield was 7948 kg ha-1 and yield ranged from 7033 kg ha-1 to 8759 kg ha-1, the highest grain yield performed by TE6744-16 line. According to the results, significant differences among cultivars in terms of plant height, days of heading, biomass, chlorophyll content, canopy temperature, glaucousness were found. TE6627-6 line had the highest chlorophyll content and also, chlorophyll content positively affected grain yield. Canopy temperature is generally related to yield under drought stress condition in bread wheat. In the study early maturing (days of heading) genotypes had lower canopy temperature. An increase in biomass after the heading phase has positively affected grain yield. In the study, no correlation was found between grain yield and biomass at GS25 and GS45 growth phase. There was a negative correlation between glaucousness with biomass at GS60, GS75 and GS85 growth phase. These results showed that physiological parameters such as biomass (at GS75 and GS85), canopy temperature (at GS60 and GS75), and chlorophyll content (at GS60), and glaucousness could be used for selection parameters under rainfed conditions for yield in bread wheat.
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50

Wu, Bin, Huichun Ye, Wenjiang Huang, Hongye Wang, Peilei Luo, Yu Ren, and Weiping Kong. "Monitoring the Vertical Distribution of Maize Canopy Chlorophyll Content Based on Multi-Angular Spectral Data." Remote Sensing 13, no. 5 (March 5, 2021): 987. http://dx.doi.org/10.3390/rs13050987.

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Remote sensing approaches have several advantages over traditional methods in determining information on physical and chemical parameters, including timely data acquisition, low costs, and wide coverage. Thus, remote sensing is widely used in crop growth monitoring. Unlike vertical observations, multi-angular remote sensing technology can obtain the vertical distribution information of the central and lower leaves of a crop. Furthermore, applications of remote sensing on the vertical distribution of maize canopy components is complicated, and related research is limited. In the current paper, we employed multi-angular spectral data, measured by a self-designed multi-angular observation instrument at view zenith angles (VZAs) of 0°, 10°, 20°, 30°, 40°, 50°, and 60°, to explore the monitoring strategy and monitoring precision of the vertical distribution of chlorophyll content in the maize canopy. This was then used to determine the optimal monitoring method for the chlorophyll content (soil and plant analyzer development (SPAD) value) of each layer. The correlation between SPAD value and chlorophyll sensitivity indices at different growth stages was used as the basis for screening indices and VZAs. The correlation between the selected EPI (eucalyptus pigment index) and REIP (red edge inflection point) indices and chlorophyll content indicated view zenith angles (VZAs) of 0°, 30°, and 40° as optimal for the early growth stage monitoring of chlorophyll content in the 1st, 2nd, and 3rd layers, respectively. These values were associated with RMSEs of 4.14, 1.71, and 1.11 for EPI, respectively; and 4.61, 2.31, and 1.00 for REIP, respectively. In addition, a VZA of 50° was selected to monitor the chlorophyll content of the 1st, 2nd, 3rd, and 4th layers at the late growth stage, with RMSE values of 2.97, 3.50, 2.80, and 4.80 for EPI, respectively; and 3.16, 5.02, 4.55, and 7.85 for REIP, respectively. The results demonstrated the ability of canopy multi-angular spectral reflectance to accurately estimate the maize canopy chlorophyll content vertical distribution, with the VZAs of different vertical layers varying between the early and late growth stages.
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