Dissertations / Theses on the topic 'Campylobacter'
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Okwumabua, Ogi Emeke. "Biochemical and molecular characterization of urease-positive campylobacters (campylobacter pylori and campylobacter mustelae." Thesis, Georgia Institute of Technology, 1989. http://hdl.handle.net/1853/25297.
Full textPratt, Alisa Annabelle. "Antibiotic Resistance Determinants of Australian Campylobacter Jejuni & Campylobacter Coli Isolates." Thesis, Griffith University, 2008. http://hdl.handle.net/10072/366198.
Full textThesis (Masters)
Master of Philosophy (MPhil)
Griffith University. School of Medical Science.
Griffith Health
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Nguyen, Hai. "Acanthamoeba-Campylobacter Interactions." Thèse, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/20172.
Full textGhaffer, Nacheervan M. "Filamentation of Campylobacter." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/35597/.
Full textGormley, Fraser James. "The epidemiology of Campylobacter jejuni and Campylobacter coli in north east Scotland." Thesis, Available from the University of Aberdeen Library and Historic Collections Digital Resources, 2008. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=25813.
Full textShaheen, Bashar Wajeeh. "In vitro survival of Campylobacter jejuni and Campylobacter coli at low Ph." Auburn, Ala., 2006. http://repo.lib.auburn.edu/2006%20Spring/master's/SHAHEEN_BASHAR_0.pdf.
Full textMcTavish, Sharla. "Comparative analysis of New Zealand campylobacter isolates using MLST, PFGE and flaA PCR RFLP genotyping : a thesis submitted to the Victoria University of Wellington in partial fulfilment of the requirements for the degree of Master of Science in Molecular Microbiology /." ResearchArchive@Victoria e-Thesis, 2008. http://hdl.handle.net/10063/413.
Full textMurphy, Helen. "Host Responses to Campylobacter." Thesis, University of Bristol, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.520636.
Full textJohn, Amy. "Campylobacter in farm animals." Thesis, University of Nottingham, 2011. http://eprints.nottingham.ac.uk/13732/.
Full textGarg, Nitanshu. "Copper, cytochromes and Campylobacter." Thesis, University of Sheffield, 2018. http://etheses.whiterose.ac.uk/22930/.
Full textRunsick, Cara Denise. "Phenotypic and genotypic characterization of several unidentified new strains of campylobacter-like bacteria." Thesis, Georgia Institute of Technology, 1989. http://hdl.handle.net/1853/25340.
Full textHorrocks, Shane Michael. "Effects of short-chain nitrocompounds against Campylobacter jejuni and Campylobacter coli in vitro." Texas A&M University, 2006. http://hdl.handle.net/1969.1/5022.
Full textDeibel, Kurt Eugene. "A study of Campylobacter jejuni /." The Ohio State University, 1985. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487260135358.
Full textBasardien, Laeeqa. "Molecular characterization of Campylobacter isolates from free range and commercial chicken in South Africa." University of the Western Cape, 2012. http://hdl.handle.net/11394/5068.
Full textCampylobacter species are the most common bacteria associated with acute diarrhoea and is responsible for 400 to 500 million reported cases globally. It is not uncommon for 35% to 85% of chicken flocks to be infected with campylobacters and it is because of this high prevalence that chicken is considered to be the primary source of Campylobacter contamination in the domestic setting. Therefore, a very high risk of acquiring campylobacteriosis is associated with the mishandling and consumption of contaminated chicken. The present study had isolated a total number of 156 Campylobacter isolates, of which 102 isolates were C. jejuni and 51 were C. coli. The speciation of 3 Campylobacter isolates could not be determined. It had shown that there is a high prevalence of Campylobacter in South African chicken. Retail chicken (n = 84) has a lower prevalence of 27% whereas chicken sampled directly from the abattoir (n = 182), but also intended for human consumption, had an average prevalence of 73%. It also showed that free range chicken (n = 118) has a higher prevalence (average of 79%) of Campylobacter than commercial chicken (n = 64) (average of 56%). It is for this reason that free range chicken is not always the safer option considering that the purchasing of free range chicken is becoming more popular for health reasons. There is no standardized universal isolation protocol for Campylobacter species and the current isolation techniques creates a bias for the optimal growth of C. jejuni and C. coli, the two thermotolerant species most commonly associated with human illnesses. Recently, the non-selective Cape Town Protocol was designed for efficient isolation of campylobacters from clinical samples and proved to be superior to the former techniques in the isolation of the thermotolerant campylobacters as well as emerging campylobacters. However, the protocol is not suited to the isolation of Campylobacter from food samples. This study successfully optimized the Cape Town protocol by incorporating the use of the selective Bolton broth for the recovery and enrichment of injured cells from raw chicken samples. The technique proved to be equal in isolation efficiency to the ISO 1272-1:2006 method but loses its ability to recover all campylobacters that may be present in the food sample. It is for this reason that a non selective enrichment broth should be sought but the technique boasts superiority over the ISO 10272-1:2006 method in that it reduces the time in obtaining the results at least by 48 h and is more cost effective.
National Research Foundation (NRF)
Stiller, Christiane. "Zum Vorkommen von Campylobacter jejuni und Campylobacter coli in Rohmilch von Erzeugerbetrieben in Nordbayern mit Versuchen zur Überlebensfähigkeit von Campylobacter jejuni in Milch." [S.l. : s.n.], 1999. http://www.diss.fu-berlin.de/1998/88/index.html.
Full textCox, Joanne Mary. "Molecular genetics of Campylobacter species." Thesis, University of Leicester, 1997. http://hdl.handle.net/2381/29782.
Full textHill, Helena Anne. "Non cultural detection of Campylobacter." Thesis, Queen's University Belfast, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.247366.
Full textStones, Leanne. "Beta-lactam resistance in Campylobacter." Thesis, University of Birmingham, 2011. http://etheses.bham.ac.uk//id/eprint/1679/.
Full textEverest, Paul Howard. "The pathogenesis of Campylobacter diarrhoea." Thesis, University of Leicester, 1992. http://hdl.handle.net/2381/34407.
Full textLau, Sok Kiang. "Molecular interrogation of Campylobacter infection." Thesis, University of Exeter, 2014. http://hdl.handle.net/10871/15425.
Full textMcGinley, Susan. "New Campylobacter Vaccine for Poultry." College of Agriculture and Life Sciences, University of Arizona (Tucson, AZ), 2008. http://hdl.handle.net/10150/622083.
Full textCAYEZ, BOIDIN BENEDICTE. "Epidemiologie des diarrhees a campylobacter." Lille 2, 1997. http://www.theses.fr/1997LIL2P037.
Full textGuthrie-Irons, Colette. "Biofilm formation in `Campylobacter jejuni'." Thesis, London School of Hygiene and Tropical Medicine (University of London), 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.536794.
Full textSemchenko, Evgeny A. "Characterisation of Campylobacter jejuni Lipooligosaccharides." Thesis, Griffith University, 2012. http://hdl.handle.net/10072/366659.
Full textThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
Institute for Glycomics
Science, Environment, Engineering and Technology
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Lewis, Sally O'Donovan Gerard A. "Development of a real-time PCR assay for the detection of Campylobacter jejuni and Campylobacter coli." [Denton, Tex.] : University of North Texas, 2009. http://digital.library.unt.edu/permalink/meta-dc-9840.
Full textLewis, Sally. "Development of a Real-time Pcr Assay for the Detection of Campylobacter Jejuni and Campylobacter Coli." Thesis, University of North Texas, 2009. https://digital.library.unt.edu/ark:/67531/metadc9840/.
Full textMerchant-Patel, Shreema. "Development of rapid and highly resolving combinatorial genotyping schemes for Campylobacter jejuni and Campylobacter coli." Thesis, Queensland University of Technology, 2009. https://eprints.qut.edu.au/33194/1/Shreema_Merchant-Patel_Thesis.pdf.
Full textRosenau-Ilias, Agnès. "Intéractions in vivo et in vitro entre Campylobacter Jejuni - Campylobacter coli et les cellules épithéliales humaines." Paris 11, 1988. http://www.theses.fr/1988PA114820.
Full textPacheco, Sophia A. "Identification of Campylobacter jejuni secreted proteins." Pullman, Wash. : Washington State University, 2010. http://www.dissertations.wsu.edu/Thesis/Spring2010/s_pacheco_021610.pdf.
Full textHani, Eric. "Hippurate hydrolase gene of Campylobacter jejuni." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0001/NQ27943.pdf.
Full textAl, Kandari Sharifa. "Characterization and comparison of Campylobacter bacteriophages." Thesis, University of Nottingham, 2013. http://eprints.nottingham.ac.uk/14529/.
Full textZilbauer, Matthias. "Innate immune defence to Campylobacter jejuni." Thesis, University College London (University of London), 2007. http://discovery.ucl.ac.uk/1445170/.
Full textNetzer, Simon Lukas [Verfasser], Oliver [Akademischer Betreuer] Einsle, and Philipp [Akademischer Betreuer] Kurz. "The alternative NrfA of Campylobacter rectus." Freiburg : Universität, 2017. http://d-nb.info/1151817643/34.
Full textMcFarland, Elizabeth Adeline. "Studies of Campylobacter isolates from poultry." Thesis, Queen's University Belfast, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335563.
Full textWainwright, Laura. "The truncated haemoglobin of Campylobacter jejuni." Thesis, University of Sheffield, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.419585.
Full textAinsworth, Paul. "Chemotaxis signal transduction in Campylobacter jejuni." Thesis, University of Leicester, 2014. http://hdl.handle.net/2381/28667.
Full textLiang, Lu. "Variation in Campylobacter phage and prophage." Thesis, University of Nottingham, 2017. http://eprints.nottingham.ac.uk/41924/.
Full textMorley, L. "Niche-adaptive evolution in Campylobacter jejuni." Thesis, Nottingham Trent University, 2014. http://irep.ntu.ac.uk/id/eprint/27913/.
Full textSpeegle, Leslie Nicole Oyarzabal Omar A. "Use of cellulose filters to isolate naturally occurring Campylobacter spp. from contaminated retail broiler meat and survival of Campylobacter jejuni and Campylobacter coli in retail broiler meat." Auburn, Ala., 2009. http://hdl.handle.net/10415/1984.
Full textPenfold, Sonya. "A molecular biological study on Campylobacter pylori." Master's thesis, University of Cape Town, 1989. http://hdl.handle.net/11427/25731.
Full textKim, Joo-Sung. "NATURAL TRANSFORMATION-MEDIATED TRANSFER OF ERYTHROMYCIN RESISTANCE IN Campylobacter coli AND Campylobacter jejuni." NCSU, 2005. http://www.lib.ncsu.edu/theses/available/etd-10272005-170447/unrestricted/etd.pdf.
Full textSchnider, Andrea. "Prevalence of "Campylobacter jejuni" and "Campylobacter coli" on Swiss broiler carcasses determined by comparative real-time PCR /." [S.l.] : [s.n.], 2009. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
Full textFlynn, Orla Mary Josephine. "Surface protein antigens of Campylobacter jejuni and their application in the differentiation of wild type Campylobacter isolates." Thesis, University of Ulster, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241476.
Full textKervella, Michèle. "Identification de protéines externes impliquées dans la fixation de "Campylobacter jejuni", "Campylobacter coli" aux cellules épithéliales humaines." Paris 11, 1990. http://www.theses.fr/1990PA114855.
Full textKiess, Aaron S. "Prevalence of Campylobacter in a turkey production facility." Morgantown, W. Va. : [West Virginia University Libraries], 2001. http://etd.wvu.edu/templates/showETD.cfm?recnum=2224.
Full textTitle from document title page. Document formatted into pages; contains viii, 90 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references (p. 59-66).
Quetz, Josiane da Silva. "Estudo sobre Campylobacter jejuni e Campylobacter coli em crianças da área urbana de Fortaleza, Ceará/Brasil : Identificação genética, inflamação intestinal e impacto no estado nutricional." reponame:Repositório Institucional da UFC, 2009. http://www.repositorio.ufc.br/handle/riufc/2445.
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Campylobacter jejuni and Campylobacter coli are important etiologic agents of worldwide diarrheal disease. Campylobacter sp. infection is usually identified by a 72 hour microbiological culture that identifies the genus of the responsible organism. Our main goal was to investigate the prevalence of C. jejuni and C. coli in children, aged 2-36 months, from urban Fortaleza, CE, Brazil, in an observational epidemiological case-control study using, as a tool of detection, the polymerase chain reaction (PCR). Our other goals were to investigate the nutritional impact of infection (cases) or colonization (controls) for Campylobacter sp., to determine the presence of three virulence genes of C. jejuni cytolethal distending toxin (CDT), and to evaluate the occurrence of inflammation in intestinal infections caused by Campylobacter sp. The study population consisted of 83 cases and 83 controls, where the cases consisted of children with a history of diarrhea in the 14 days prior to selection for the study. We assessed socioeconomic parameters through an epidemiological questionnaire. Anthropometric measurements were collected to determine z-score parameters for assessing the nutritional status of the children. Detection of Campylobacter from frozen samples was performed by enzyme-linked immunosorbent assay (ELISA) and PCR. Also, using PCR technology, we investigated the presence of C. jejuni genes cdtA, cdtB and cdtC. Intestinal inflammation was assessed by semi-quantitative ELISA detection of fecal lactoferrin (LFF). PCR technology detected C. jejuni in 9.6% of the cases (8/83) and 7.2% of the controls (6/83), while C. coli was detected in 6.0% of the cases (5/83) and 1.2% of the controls (1/83). CDT genes were found in 50% of hipO+ samples (7/14). There was a significant difference (p <0.05) in the weight for age z-scores (WAZ) and the weight for height z-scores (WHZ) between case and control carriers of C. jejuni, where case carriers showed lower average WAZ and WHZ than control carriers. Moreover, in the case group, carriers of C. jejuni showed a lower WHZ average than that of non-carrier cases of C. jejuni. More than 80.0% of the children studied had intestinal inflammation characterized by high levels of LFF regardless of the presence of diarrhea and Campylobacter sp. In conclusion, our findings corroborate data in the scientific literature related to the prevalence of C. jejuni and C. coli in pediatric populations, the existence of asymptomatic carriers and an association between the detection of the microorganism and malnutrition. In addition, our data suggest a genetic variability among the strains of C. jejuni detected in the study population, related to presence o absence of CDT genes.
Campylobacter jejuni e Campylobacter coli são importantes agentes etiológicos de doença diarréica na população mundial. A infecção por Campylobacter sp. é usualmente identificada por cultivo microbiológico que leva aproximadamente 72 horas para identificação do gênero. Nosso objetivo principal foi pesquisar a prevalência de C. jejuni e C. coli em população infantil, com idade entre 2-36 meses, da área urbana de Fortaleza/CE, Brasil, em estudo do tipo epidemiológico observacional caso-controle, utilizando, como ferramenta de detecção, a reação em cadeia da polimerase (PCR). Outros objetivos consistiram em: investigar o impacto nutricional da infecção (casos) ou da colonização (controles) por Campylobacter sp.; determinar a presença de três genes de virulência para a toxina citoletal distensora (CDT) de C. jejuni e avaliar a ocorrência de inflamação intestinal nas infecções causadas por Campylobacter sp. A população estudada consistiu de 83 casos e 83 controles, sendo os casos, crianças com histórico de diarréia nos 14 dias pregressos à seleção para o estudo. Foram avaliados parâmetros sócio-econômicos através de questionário epidemiológico. Medidas antropométricas foram coletadas para determinação de escores-z no intuito de avaliar o perfil nutricional das crianças. A detecção de Campylobacter nas amostras congeladas foi realizada por ensaio imuno-enzimático (ELISA) e PCR. Pela PCR também investigamos a presença dos genes cdtA, cdtB e cdtC da CDT de C. jejuni. A avaliação da inflamação intestinal foi realizada pela pesquisa de lactoferrina fecal (LFF), através de ELISA semiquantitativa. Foi detectado, por PCR, C. jejuni em 9,6% dos casos (8/83) e 7,2% dos controles (6/83). C. coli foi detectado em 6,0% dos casos (5/83) e 1,2% dos controles (1/83). Os genes cdtA, cdtB e cdtC foram encontrados em 50% das amostras hipO+ (7/14). Houve diferença significativa (p<0,05) dos escores WAZ e WHZ entre casos e controles portadores de C. jejuni, sendo que casos portadores apresentaram média inferior de WAZ e WHZ, quando comparados com os controles portadores. No grupo Casos, os portadores de C. jejuni apresentavam valor médio de WHZ inferior ao valor médio apresentado pelos casos não-portadores. Mais de 80,0% das crianças estudadas apresentaram inflamação intestinal caracterizada por elevados níveis de LFF, independente da presença de diarréia e Campylobacter sp. Em conclusão, nossos achados corroboram dados da literatura científica relacionados à prevalência de C. jejuni e C. coli na população infantil, existência de portadores assintomáticos e associação entre a detecção do microorganismo e desnutrição. Além disso, nossos dados apontam para ocorrência de variabilidade genética dentre as cepas de C. jejuni detectadas na população estudada em relação à presença ou ausência dos genes de CDT.
Chandan, Vandana Carleton University Dissertation Biology. "Development of simple and economical methods for culturing campylobacter and for producing anti-campylobacter antibodies for enzyme immunoassay." Ottawa, 1991.
Find full textBento, Kérley Braga Pereira. "Padronização de PCR tradicional e em tempo real para detecção de Campylobacter jejuni e Campylobacter coli em alimentos." Universidade Estadual de Londrina, 2010. http://www.bibliotecadigital.uel.br/document/?code=vtls000157832.
Full textCampylobacter jejuni and Campylobacter coli are frequently associated with human campylobacteriosis, with more than 80% of infections caused by C. jejuni. The infection is sporadic and outbreaks are rare. In addition to gastroenteritis, C. jejuni has also been associated to autoimmune diseases post-infection including arthritis, Guillain-Barré syndrome (GBS), Miller-Fisher syndrome and Reiter syndrome. Poultry and poultry products have been implicated as the major vehicles for Campylobacter infection. Conventional methods for the isolation and identification of Campylobacter from food products usually require enrichment culture and subculture on selective agar. These methods are time-consuming and after the isolation, phenotypic identification requires additional biochemical and serological tests. Molecular methods such as polymerase chain reaction (PCR) have been standardized for detection, identification and quantification of Campylobacter spp. in foods because they are rapid, sensitive and specific. The objectives of this study were to develop a multiplex PCR for detection and differentiation between C. jejuni and C. coli in chicken carcasses; moreover, quantify C. jejuni in pure cultures and detect C. jejuni with spiked and naturally contaminated chicken carcasses by real time PCR. The multiplex PCR was developed using mapA primers specific for detection of C. jejuni and ceuE primers specific for detection of C. coli. The multiplex PCR developed was tested on 11 different isolates of Campylobacter and on 22 non-Campylobacter species and specificity of the assay standardized was 100%. The PCR assay was tested with spiked and naturally contaminated chicken carcasses. Campylobacter was detected from chicken skin spiked contaminated with approximately 50 colony forming unit (CFU) of Campylobacter per 10-g after 48 h of selective enrichment. Specific DNA fragments of the PCR confirmed the presence of C. jejuni (202-bp) and or C. coli (889-pb) from thirteen (46.4%) out of 28 analyzed chicken carcasses purchased from local retail market, in Londrina. For real-time PCR assays were used mapA primers specific for detection of C. jejuni. Tests for specificity of real-time PCR using the gene mapA for isolates of Campylobacter and other bacterial genera showed that only isolates of C. jejuni produced PCR products with a peak separation of 76.3 ° C. The limit detection of qPCR was estimated at about one copy of DNA by PCR for pure cultures of C. jejuni. The real-time PCR assay was tested with spiked and naturally contaminated chicken carcasses. C. jejuni was detected from chicken skin spiked with approximately 1, 10 or 50 colony forming unit (CFU) of C. jejuni per 10-g of skin after 48 h of selective enrichment. Specific PCR products were observed in all samples analyzed by the real-time PCR after 48 h of selective enrichment when DNA was extracted by boiling using Triton X-100. The PCR multiplex and real-time PCR methods have shown to be rapid, sensitive and specific on the detection of Campylobacter in chicken carcasses, when compared with conventional methods that requires 5 days for a negative result and 6-7 days to confirm a positive result.
Silva, Lidiane Azevedo. "Pesquisa de Campylobacter jejuni e Campylobacter coli em espécimes fecais de crianças com diarreia aguda e sem diarreia." Universidade Federal de Minas Gerais, 2012. http://hdl.handle.net/1843/BUOS-95DQ5B.
Full textA doença diarreica aguda é considerada um problema de saúde pública associado a taxas elevadas de morbimortalidade, que acomete, especialmente, a população pediátrica de nível socioeconômico baixo. Entre os agentes bacterianos diarreiogênicos, merece menção Campylobacter, com destaque para as espécies Campylobacter jejuni e Campylobacter coli. Embora a relevância do organismo seja reconhecida, estudos nacionais referentes à sua prevalência são bastante escassos. O objetivo desta investigação foi avaliar a participação de C. jejuni e C. coli na etiologia da enterite aguda e a existência de associação entre infecção por Campylobacter e parâmetros demográficos e clínicos. Foram incluídas no estudo espécimes fecais de 764 crianças, 363 com diarreia aguda e 401 sem diarreia, de nível socioeconômico baixo, com até 69 meses de idade, atendidas no Hospital Infantil João Paulo II, entre 2004 e 2007. A pesquisa de C. jejuni e C. coli foi realizada por PCR, empregando-se DNA extraído diretamente das fezes, sendo selecionados dois protocolos previamente propostos. Infecção por C. jejuni e C. coli foi observada em 2,2% e 1,1% do grupo caso, com predomínio (66,7%) de C. jejuni. Nenhuma criança sem diarreia apresentou-se colonizada pelo organismo. Infecção por Campylobacter não estava associada a sexo e faixa etária da criança nem apresentou variação temporal ou sazonal. No que se refere aos parâmetros clínicos, presença de Campylobacter estava associada a maior frequência de evacuações e presença de sangue nas fezes, características clínicas do quadro de diarreia inflamatória. Os dados indicaram redução da taxa de diarreia associada a Campylobacter no nosso meio.
Adler, Linda [Verfasser]. "Autoinducer 2 in Campylobacter jejuni / Linda Adler." Berlin : Freie Universität Berlin, 2016. http://d-nb.info/1096221179/34.
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