Journal articles on the topic 'C-PSW'
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1
Banevičienė, Malaiškienė, Vaičienė, and Boris. "The Possibilities of Paper Sludge Waste (PSw) Utilization in Cement Materials." Proceedings 16, no. 1 (July 4, 2019): 34. http://dx.doi.org/10.3390/proceedings2019016034.
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The paper production is developed throughout the world, therefore, more paper sludge waste (PSw) is accumulating. The aim of this work was to determine the properties of PSw burned at 900 °C and its impact on physical–mechanical properties of cementitious matrix and evaluate its usage/utilization possibilities in cementitious materials. For the implementation of the aim, cement-based specimens were prepared and their –mechanical properties were determined. Specimens from five compositions were formed, with 2.5%, 5%, 7.5%, and 10% of the cement by weight replaced with PSw. When 5% of the cement was replaced with PSw, the compressive strength increased by approximately 7%, although density and ultrasound velocity slightly decreased (up to 1.5%). When 7.5% PSw was added, the compressive strength of the specimens decreased. To summarize, it can be stated that up to 5% PSw may be utilized/used for the preparation of cementitious mixtures. The usage of the waste allows environment conservation, reduced amount of cement in mixtures, and improvement of properties of cementitious materials.
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Ives, S. W., L. A. Sparrow, W. E. Cotching, R. B. Doyle, and S. Lisson. "N Mineralisation from Bioresources Incubated at 12.5°C." Applied and Environmental Soil Science 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/803736.
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Soils treated with lime-amended biosolids (LAB), poppy seed waste (PSW), anaerobically digested biosolids (ADB) and poppy mulch (PM) and incubated at 12.5°C for 56 days released 45%, 36%, 25%, and −8%, respectively, of total applied N as plant available nitrogen (PAN) by the end of the incubation. The mineralisation rates were contrary to expectations based on the C : N ratios of the four products: LAB (5 : 1), PSW (7 : 1), ADB (3 : 1), and PM (16 : 1). PM showed a significant negative priming effect over the incubation period. These results have implications for production agriculture in temperate regions where application and incorporation of bio-resources traditionally occurs in autumn and spring when soil and air temperatures are relatively low. Current application times may not be suitable for nitrogen release to satisfy crop demand.
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Whitehead, James R. "A Study of Children’s Physical Self-Perceptions Using an Adapted Physical Self-Perception Profile Questionnaire." Pediatric Exercise Science 7, no. 2 (May 1995): 132–51. http://dx.doi.org/10.1123/pes.7.2.132.
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This project was a study of the validity and reliability of adapted versions of Fox and Corbin’s (10) Physical Self-Perception Profile (PSPP) and Perceived Importance Profile (PIP) for use with seventh- and eight-grade students. The Children’s PSPP and PIP (C-PSPP and C-PIP) questionnaires were completed by 505 students. Results supported the reliability and the construct and concurrent validity of the C-PSPP scales. Factorial validity of the C-PIP was not demonstrated. Similar to Fox and Corbin’s (10) results, regression analysis revealed that a large proportion of the variance in general physical selfworth (PSW) was explained by the C-PSPP scales. The hypothesis that PSW mediates between general self-worth (GSW) and the four C-PSPP scales in a hierarchical arrangement was also supported. Failure to psychologically discount the importance of perceived incompetence in specific areas impacted global self-worth. Correlations with physical fitness test scores provided evidence of concurrent validity of the C-PSPP scales.
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Beaujean, A., Nicholas Benson, Ryan McGill, and Stefan Dombrowski. "A Misuse of IQ Scores: Using the Dual Discrepancy/Consistency Model for Identifying Specific Learning Disabilities." Journal of Intelligence 6, no. 3 (August 1, 2018): 36. http://dx.doi.org/10.3390/jintelligence6030036.
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The purpose of this article is to describe the origins of patterns of strengths and weaknesses (PSW) methods for identifying specific learning disabilities (SLD) and to provide a comprehensive review of the assumptions and evidence supporting the most commonly-used PSW method in the United States: Dual Discrepancy/Consistency (DD/C). Given their use in determining whether students have access to special education and related services, it is important that any method used to identify SLD have supporting evidence. A review of the DD/C evidence indicates it cannot currently be classified as an evidence-based method for identifying individuals with a SLD. We show that the DD/C method is unsound for three major reasons: (a) it requires test scores have properties that they fundamentally lack, (b) lack of experimental utility evidence supporting its use, and (c) evidence supporting the inability of the method to identify SLD accurately.
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Kizilarslan, Emre, and Michel Bruneau. "Hysteretic behavior of repaired C-shaped concrete filled-composite plate shear walls (C-PSW/CF)." Engineering Structures 241 (August 2021): 112410. http://dx.doi.org/10.1016/j.engstruct.2021.112410.
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Harmon, Joshua R., and Amit H. Varma. "Local buckling of steel faceplates anchored to concrete infill in C-PSW/CF." Thin-Walled Structures 167 (October 2021): 108230. http://dx.doi.org/10.1016/j.tws.2021.108230.
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Kizilarslan, Emre, Morgan Broberg, Soheil Shafaei, Amit H. Varma, and Michel Bruneau. "Non-linear analysis models for Composite Plate Shear Walls-Concrete Filled (C-PSW/CF)." Journal of Constructional Steel Research 184 (September 2021): 106803. http://dx.doi.org/10.1016/j.jcsr.2021.106803.
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Shafaei, Soheil, Amit H. Varma, Morgan Broberg, and Ron Klemencic. "Modeling the cyclic behavior of composite plate shear walls/concrete filled (C-PSW/ CF)." Journal of Constructional Steel Research 184 (September 2021): 106810. http://dx.doi.org/10.1016/j.jcsr.2021.106810.
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Wang, Huafei, Qiang Gu, and Yi Qi. "Thickness demand for concrete panel in design of C-PSW/CE under cyclic loading." Journal of Building Engineering 48 (May 2022): 104011. http://dx.doi.org/10.1016/j.jobe.2022.104011.
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Dlangamandla, C., S. K. O. Ntwampe, and M. Basitere. "A bioflocculant-supported dissolved air flotation system for the removal of suspended solids, lipids and protein matter from poultry slaughterhouse wastewater." Water Science and Technology 78, no. 2 (July 23, 2018): 452–58. http://dx.doi.org/10.2166/wst.2018.324.
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Abstract In this study, two previously identified isolates, i.e. Comamonas aquatica (BF-3) and Bacillus sp. BF-2, were determined to be suitable candidates to utilise in a bioflocculant-supported dissolved air flotation (Bio-DAF) system as a pretreatment system for poultry slaughterhouse wastewater (PSW). A 2% (v/v) (bioflocculant:PSW) strategy was used for the DAF to reduce total suspended solids (TSS), lipids and proteins in the PSW, by supplementing the bioflocculants produced and the co-culture (C. aquatica BF-3 and Bacillus sp. BF-2) directly into the DAF. The Bio-DAF was able to reduce 91% TSS, 79% proteins and 93% lipids when the DAF system was operating at steady state, in comparison with a chemical DAF operated using 2% (v/v) alum that was able to only reduce 84% TSS, 71% proteins and 92% lipids. It was concluded that the Bio-DAF system worked efficiently for the removal of suspended solids, lipids and proteins, achieving better results than when alum was used.
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O’Gorman, Tessa, John S. Lambert, Tina McHugh, Walter Cullen, Gordana Avramovic, Raffaele Federico, Bernard West, et al. "HepCare Plus: Enhancing Primary Care Identification and Treatment of Hepatitis C Virus in High-Risk Individuals." Pathogens 11, no. 12 (November 27, 2022): 1428. http://dx.doi.org/10.3390/pathogens11121428.
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Hepatitis C Virus (HCV) disproportionately affects people who inject drugs, migrants, prisoners and the homeless. An integrated, peer-led model of care involving primary and secondary care is required to enhance the identification and treatment of HCV in these marginalised groups. HepCare Plus builds on the network and achievements of HepCare Europe (a co-funded Third Health Programme of the European Union/Health Service Executive project). It further identifies those not accessing care and facilitates prompt assessment and treatment of those diagnosed with HCV, with the aid of a peer support worker (PSW) and a community HCV nurse specialist. Of 109 individuals identified and assessed for HCV treatment, 100 commenced HCV treatment. Despite interruptions to treatment (COVID-19 pandemic and national health service cyberattack) there was a high-level of treatment completion with PSW engagement (98%, n = 98). Eighty (73%) individuals were previously aware of a positive HCV status, highlighting the ongoing need to address barriers preventing marginalised groups from engaging with care. HepCare Plus reiterates the defining role of peer-led community interventions in HCV treatment engagement and the need for continuous open-ended HCV care. It provides a sustainable framework to meaningfully combat HCV and achieve the United Nations Sustainable Development Goal of HCV elimination by 2030.
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Banevičienė, Vilma, Jurgita Malaiškienė, Renata Boris, and Jiri Zach. "The Effect of Active Additives and Coarse Aggregate Granulometric Composition on the Properties and Durability of Pervious Concrete." Materials 15, no. 3 (January 28, 2022): 1035. http://dx.doi.org/10.3390/ma15031035.
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Pervious concrete (PCO) has many advantages and applications, such as water pooling reduction, noise attenuation, replenishment of groundwater reserves, etc. However, the use of pervious concrete is limited due to its low compressive strength and durability, especially as a result of portlandite leaching from concrete exposed to flowing water. The effects of active additives (nano SiO2 (NS) spent catalyst generated at the fluid catalytic cracking unit (FCCCw) and paper sludge waste burned at 700 °C (PSw)) along with particle size distribution of the coarse aggregate on the properties and durability of pervious concrete were determined in the research. Active additives used in the binder were found to reduce portlandite leaching from concrete exposed to flowing water to significantly increase the resistance of concrete to freezing and thawing cycles and to increase sound absorption, compressive strength and infiltration rate. In addition, industrial waste (FCCCw and PSw) used as active additives significantly reduced the use of clinker in concrete applied in the construction of water pervious systems. The coarse aggregate size distribution had the greatest effect on the density, ultrasound pulse velocity (UPV), porosity, compressive strength and infiltration rate of pervious concrete.
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Ntambwe Kamangu, Erick. "Subtypes of the Human Immunodeficiency Virus Circulating in Professional Sex Workers Community: A Different Epidemiology." Archives of Medical Case Reports and Case Study 5, no. 4 (April 25, 2022): 01–03. http://dx.doi.org/10.31579/2692-9392/102.
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Background: HIV-1 has a genetic diversity that is equal to the complexity of its management. Professional Sex Workers (PSW) have always been considered as a key population for Sexually Transmitted Infections around the world. They are often incriminated for transporting and introducing cosmopolitan subtypes into populations. Objective: The objective of this review was to present the different strains of Human Immunodeficiency Virus type 1 (HIV-1) which circulate in the population of Professional Sex Workers in Kinshasa, Democratic Republic of Congo (DRC) and their evolution. Methods: Various publications related to the identification of the different variants of HIV-1 among PSW in Kinshasa-DRC were the subject of this literature review. The research for these different works on the different variants of type 1 HIV was done on the internet from websites using specific keywords. The search was limited to published work and abstracts presented from 2000 to date. The manuscripts were selected according to the relevance of the methodology, the results as well as the representativeness of the samples. The socio-demographic information of the populations studied, the measurement methods and the objectives were taken into account in the evaluation of the articles. Results: From 2000 to 2021, 2 works were documented carried out mainly on the PSWs for Kinshasa-DRC meeting the various selection criteria. In 2012, according to the IBBS, subtype A was dominant with 4 PSWs (30.8%), followed by subtypes G with 2 PSWs (15.4%) and C with 1 PSW (7.7%). In 2014, according to Kamangu NE et al, the dominant subtype was K in 5 patients (25%), followed by subtypes A and G in 3 patients each (15%). Conclusion: It emerges from this work that this key population is a group with a complex and special epidemiology. The prevalence of subtype A has been declining over the years while other subtypes are on the rise.
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Taghipour Anvari, Ataollah, Saahastaranshu R. Bhardwaj, Shivam Sharma, and Amit H. Varma. "Performance of Composite Plate Shear Walls/Concrete Filled (C-PSW/CF) Under Fire Loading: A Numerical Investigation." Engineering Structures 271 (November 2022): 114883. http://dx.doi.org/10.1016/j.engstruct.2022.114883.
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Woolbright, David, Vladimir Zanev, and Neal Rogers. "VisibleZ: A Mainframe Architecture Emulator for Computing Education." Serdica Journal of Computing 8, no. 4 (October 2, 2015): 389–408. http://dx.doi.org/10.55630/sjc.2014.8.389-408.
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This paper describes a PC-based mainframe computer emulatorcalled VisibleZ and its use in teaching mainframe Computer Organizationand Assembly Programming classes. VisibleZ models IBM’s z/Architectureand allows direct interpretation of mainframe assembly language objectcode in a graphical user interface environment that was developed in Java.The VisibleZ emulator acts as an interactive visualization tool to simulateenterprise computer architecture. The provided architectural componentsinclude main storage, CPU, registers, Program Status Word (PSW), andI/O Channels. Particular attention is given to providing visual clues tothe user by color-coding screen components, machine instruction execution,and animation of the machine architecture components. Students interact with VisibleZ by executing machine instructions in a step-by-stepmode, simultaneously observing the contents of memory, registers, and changes inthe PSW during the fetch-decode-execute machine instruction cycle. Theobject-oriented design and implementation of VisibleZ allows students todevelop their own instruction semantics by coding Java for existing specificz/Architecture machine instructions or design and implement new machineinstructions. The use of VisibleZ in lectures, labs, and assignments is describedin the paper and supported by a website that hosts an extensivecollection of related materials. VisibleZ has been proven a useful tool inmainframe Assembly Language Programming and Computer Organizationclasses. Using VisibleZ, students develop a better understanding of mainframe concepts, components, and how the mainframe computer works.ACM Computing Classification System (1998): C.0, K.3.2.
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Smidt, Mary L., and Anne K. Vidaver. "Isolation and characterization of syringacin W-1, a bacteriocin produced by Pseudomonas syringae pv. syringae." Canadian Journal of Microbiology 32, no. 3 (March 1, 1986): 231–36. http://dx.doi.org/10.1139/m86-046.
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Syringacin W-1, a bacteriocin produced by Pseudomonas syringae pathovar syringae strain PsW-1, is a 20 × 75 nm rod-shaped particle composed of an inner core and outer sheath. Production of syringacin W-1 in broth was induced with 0.1 μLg/mL mitomycin C. The bacteriocin was purified from culture lysates using ultrafiltration, rate zonal centrifugation in sucrose gradients, and DEAE-cellulose chromatography. Purity was evaluated by subjecting syringacin W-1 preparations to electrophoresis in polyacrylamide gels under nondenaturing and denaturing conditions. The chemical composition was principally protein (67.2%), and also comigrating nonessential carbohydrate (10–35%). The physical properties of purified syringacin W-1 were a sedimentation coefficient of 104 for rod-shaped particles, pH stability of 5.2–8.2, and temperature stability from −20 to 40 °C. The bacteriocin was resistant to proteases and to 12 of 13 surfactants tested.
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Kiefer, Zoe, Amanda Chipman, Jamie M. Studer, Rafael Dal Forno Gianluppi, Aileen F. Keating, Nicholas Gabler, and Jason W. Ross. "28 Steroid hormones are differentially abundant in sows with elevated risk for pelvic organ prolapse during late gestation." Journal of Animal Science 98, Supplement_3 (November 2, 2020): 113. http://dx.doi.org/10.1093/jas/skaa054.191.
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Abstract Sow mortality, as the result of pelvic organ prolapse (POP), has increased during recent years in the U.S. swine industry and continues to worsen. The objective of this study was to identify putative biological factors associated with POP risk. We hypothesized sows differing in POP risk would differ in serum factors and steroid hormone profiles. Perineal scores (PS) were assigned to sows (n=2865) during late gestation (days 107-116) to assess POP risk. Sows assigned a PS1 had a presumed low risk of POP while those assigned a PS2 or PS3 had a presumed moderate or high risk of POP, respectively. Subsequently, 1.0, 2.7, and 23.4% of sows scored as PS1, PS2, or PS3, experienced POP, respectively. Blood samples were collected on a subset of sows (n=215) identified as PS3 along with parity-matched PS1 sows at the time of scoring and whole blood was subjected to complete blood count (CBC) analysis. Serum from a subset of these (n=110) samples were also subjected to a 17-panel hormone analysis. Additionally, serum biomarkers of inflammation, C-reactive protein and haptoglobin, were analyzed in PS3 sows that subsequently experienced POP and parity matched PS1 sows that did not experience POP. The CBC analysis revealed a 4.3, 7.8 and 5.9% decrease (P < 0.05) in mean platelet volume, lymphocytes, and monocytes, respectively, in PS3 compared to PS1 sows. Deoxycortisol, androstenedione, androsterone, estrone, β-estradiol, and testosterone were greater (P < 0.05) in PS3 compared to PS1 sows. Circulating haptoglobin was unaffected (P = 0.35) by PS, although C-reactive protein was 24.6% greater (P = 0.02) in PS3 sows. Collectively, serum differences in hormone profiles and biomarkers consistent with inflammation in sows differing in POP risk. This project was supported by the National Pork Board and the Foundation for Food and Agriculture Research.
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Vargas Santillán, A., J. C. Farias Sanchez, M. G. Pineda Pimentel, and A. J. Castro Montoya. "Olefins and Ethanol from Polyolefins: Analysis of Potential Chemical Recycling of Poly(ethylene) Mexican Case." International Journal of Chemical Reactor Engineering 14, no. 6 (December 1, 2016): 1289–300. http://dx.doi.org/10.1515/ijcre-2015-0217.
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Abstract Plastic solid waste (PSW) presents challenges and opportunities to society regardless of their sustainability awareness and technological advances. A special emphasis is paid on waste generated from polyolefin sources, which makes up a great percentage of our daily commodities’ plastic products. In Mexico 7.6 millions of tons of plastic in 2012 were wasted, which low density polyethylene LDPE, and high density polyethylene HDPE were the most abundant. Increasing cost, and decreasing space of landfills are forcing considerations of alternative options for PSW disposal. Years of research, study and testing have resulted in a number of treatment, recycling and recovery methods for plastics that can be economically, and environmentally viable. The following work studies the possibilities of polyethylene recycling. Nowadays, non-catalytic thermal cracking (Pyrolysis) is receiving renewed attention, due to the fact of added value on a crude oil barrel and its very valuable yielded products, but a fact remains that advanced thermo-chemical recycling of polyolefin still lacks the proper design, and kinetic background to target certain desired products and/or chemicals. On the other hand some research have shown a good performance that can be used in a real plant. ASPEN Plus is used to simulate a non-catalytic thermal cracking process. The process behavior of simulation is similar to the experimental data from other authors. Using gibbs free energy to identify the chemical equilibrium in system, its global minimization allows identifying the amount of substances present in the process. The simulation results demonstrate that it could be produced 49 % and 34 % wt of ethylene and propylene respectively from gas yield at 850 °C. Then scale the plant to produce ethylene and propylene from the pyrolysis and ethanol from a direct hydration of ethylene. Aspen Process Economics Analyzer is used in order to find the feasibility of the pyrolysis and ethanol production. The total sales/total production cost ratio obtained for the integrated process approaches was 2.55.
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Njoya, M., M. Basitere, and S. K. O. Ntwampe. "Treatment of poultry slaughterhouse wastewater using a down-flow expanded granular bed reactor." Water Practice and Technology 14, no. 3 (June 5, 2019): 549–59. http://dx.doi.org/10.2166/wpt.2019.039.
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Abstract This study evaluated the performance of a novel high rate anaerobic bioreactor system for the treatment of poultry slaughterhouse wastewater (PSW). The new system consisted of a granule-based technology operated in a down-flow configuration, with the assistance of medium-sized pumice stones used as packing materials for the retention of the anaerobic granules, to avoid challenges associated with the use of the three-phase separator of up-flow systems and the washout of the anaerobic biomass. Furthermore, a recycling stream was applied to the system to improve the mixing inside the Down-flow Expanded Granular Bed Reactor (DEGBR), i.e. the influent distribution to the granular biomass, and the implementation of intermittent fluidization when required to alleviate the effects of pressure drop in such systems. The DEGBR was operated under mesophilic conditions (30–35 °C) and achieved total chemical oxygen demand (tCOD), five-day biological oxygen demand and total suspended solids average removal percentages >95%, and a fats, oils and grease average removal percentage of 93.67% ± 4.51, for an organic loading rate varying between 1.1 to 38.9 gCOD/L.day.
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Miciak, Jeremy, W. Pat Taylor, Karla K. Stuebing, and Jack M. Fletcher. "Simulation of LD Identification Accuracy Using a Pattern of Processing Strengths and Weaknesses Method With Multiple Measures." Journal of Psychoeducational Assessment 36, no. 1 (December 20, 2016): 21–33. http://dx.doi.org/10.1177/0734282916683287.
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We investigated the classification accuracy of learning disability (LD) identification methods premised on the identification of an intraindividual pattern of processing strengths and weaknesses (PSW) method using multiple indicators for all latent constructs. Known LD status was derived from latent scores; values at the observed level identified LD status for individual cases according to the concordance/discordance method. Agreement with latent status was evaluated using (a) a single indicator, (b) two indicators as part of a test–retest “confirmation” model, and (c) a mean score. Specificity and negative predictive value (NPV) were generally high for single indicators (median specificity = 98.8%, range = 93.4%-99.7%; median NPV = 94.2%, range = 85.6%-98.7%), but low for sensitivity (median sensitivity = 49.1%, range = 20.3%-77.1%) and positive predictive value (PPV; median PPV = 48.8%, range = 23.5%-69.6%). A test–retest procedure produced inconsistent and small improvements in classification accuracy, primarily in “not LD” decisions. Use of a mean score produced small improvements in classifications (mean improvement = 2.0%, range = 0.3%-2.8%). The modest gains in agreement do not justify the additional testing burdens associated with incorporating multiple tests of all constructs.
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Polat, Erkan. "Boundary plate influence on tie bars axial force demands in composite plate shear walls‒concrete filled." Challenge Journal of Structural Mechanics 8, no. 4 (December 13, 2022): 166. http://dx.doi.org/10.20528/cjsmec.2022.04.005.
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In composite plate shear walls–concrete filled (C-PSW/CF), there is an indeterminate flow of force between concrete, steel plate and tie bars. Finite element methods (FEM) are frequently used to verify this force flow. The theoretical models available in the literature to predict the tie bar maximum axial force demands were based on walls without boundary plates. The finding in this study is intended to help understand whether current theoretical approaches are conservative and can be applied to boundary plate walls as well. Within the scope of this study, tie bar axial force demands for walls with boundary plates consisting of planar and round shapes and without boundary plates were investigated and compared. For this, a previously benchmarked finite element (FE) wall model was considered and configured to have no boundary plate and have planar and round boundary plates. FE models were analyzed under monotonic lateral displacement up to 4% drift ratio. Passive lateral pressures and transverse force variations on the planar and round boundary plates were investigated.
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Rodríguez-Lorenzo, Luis M., R. García-Carrodeguas, M. A. Rodríguez, S. De Aza, J. Jiménez, A. López-Bravo, M. Fernandez, and Julio San Román. "Wollastonite-Poly(Ethylmethacrylate-Co-Vinylpyrrolydone) Nanostructured Materials: Mechanical Properties and Biocompatibility." Key Engineering Materials 309-311 (May 2006): 1149–52. http://dx.doi.org/10.4028/www.scientific.net/kem.309-311.1149.
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Synthetic pseudowollastonite (psW) and a nanostructured copolymer made of a biostable component, Poly(ethylmethacrylate) (PEMA) and a bioresorbable component, vinylpyrrolidone (VP) are used in this work for the preparation of a new family of bone substitutes that allow osseointegration and mechanical stability. Composites are prepared by bulk polymerization of the desired composition in 15 mm diameter cylindrical plastic moulds. Polymerization was induced thermally at 50°C using 1wt% azobis(isobutyronitrile) (AIBN) as free-radical initiator. The moulds were filled to a height of 100 mm and 1 mm height discs were cut with a diamond saw. Specimens with a ceramic/polymer ratio 58/42, 33/67,17/83 and 0/100 were obtained. Compression stress in the range 39-59 MPa and elastic modulus between 2.64 and 4.14 GPa are obtained where the greater values correspond to the specimens prepared with a 60% ceramic load. Degradation in SBF produces a porous nanostructure in the polymeric component indicating microdomains of different solubility and the formation of an apatite-like layer on the surface of the wollastonite component. All the compositions assayed present a biocompatibility at least of the level or even superior than the Thermanox® control used.
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Mahadevan, Daruka, Geoffrey Shapiro, Sandra E. Kurtin, James M. Cleary, John F. Lyons, Ana Rodriguez-Lopez, Murray Yule, et al. "Activity of AT13387, a novel, non-ansamycin inhibitor of heat shock protein 90, against gastrointestinal stromal tumors (GIST)." Journal of Clinical Oncology 31, no. 4_suppl (February 1, 2013): 105. http://dx.doi.org/10.1200/jco.2013.31.4_suppl.105.
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105 Background: AT13387 is a second-generation potent, novel non-ansamycin HSP90 inhibitor (Kd 0.71nM). The majority of GIST tumors are characterized by activating mutations of c-KIT, an HSP90 client protein. Secondary resistance mutations within c-KIT limit clinical responses to TKIs. The dependence of c-KIT and its mutated forms on HSP90 suggests that HSP90 inhibition may be a valuable treatment option for imatinib-sensitive and resistant clones. In vitro, AT13387 inhibited the proliferation of imatinib-sensitive (GIST882, GIST-T1) and imatinib-resistant (GIST430, GIST48) cell lines. In vivo, AT13387 demonstrated anti-tumor activity in the imatinib-sensitive (GIST-PSW) and imatinib-resistant (GIST430) xenograft models. Induction of HSP70, depletion of phospho-c-KIT and inhibition of c-KIT signaling were observed in both models. Combination treatment of imatinib and AT13387 in the GIST430 model was well tolerated and significantly enhanced tumor growth inhibition over either monotherapies. Methods: In a completed phase I study, AT13387 was administered IV over 1 hour twice weekly or weekly of a 28-day cycle in a standard 3+3 dose-escalation design. The primary endpoint was to determine the MTD; secondary endpoints included PK, PD, safety and tolerability. Results: The PK exposures were dose-dependent and linear. AT13387 was well tolerated on both schedules. DLTs included primarily G2 AEs of GI toxicities, fatigue and infusion site reactions. The once weekly RP2D was determined to be 260 mg/m2. HSP70 induction was 2–7 fold at higher doses. A total of 7 GIST subjects were enrolled. An objective and durable PR was observed in one subject and 2 SDs at 8, 7 and 11 months, respectively. The PR subject demonstrated molecular resistance to kinase inhibitor treatment in the c-KIT gene prior to initiation of AT13387 therapy in two resected lesions by harboring the same activating c-KIT deletion in exon 11 and two separate TKI resistance mutations in exon 17. Conclusions: Overall, these results suggest AT13387 is a promising agent in GIST, including TKI-resistant c-Kit positive GIST. AT13387 is currently being evaluated in combination with imatinib in an ongoing phase I/II study. Clinical trial information: NCT01294202.
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Ma, Amy, Alan Richardson, Erik M. Schaefer, and J. Thomas Parsons. "Serine Phosphorylation of Focal Adhesion Kinase in Interphase and Mitosis: A Possible Role in Modulating Binding to p130Cas." Molecular Biology of the Cell 12, no. 1 (January 2001): 1–12. http://dx.doi.org/10.1091/mbc.12.1.1.
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Focal adhesion kinase (FAK) is an important regulator of integrin signaling in adherent cells and accordingly its activity is significantly modulated during mitosis when cells detach from the extracellular matrix. During mitosis, FAK becomes heavily phosphorylated on serine residues concomitant with its inactivation and dephosphorylation on tyrosine. Little is known about the regulation of FAK activity by serine phosphorylation. In this report, we characterize two novel sites of serine phosphorylation within the C-terminal domain of FAK. Phosphorylation-specific antibodies directed to these sites and against two previously characterized sites of serine phosphorylation were used to study the regulated phosphorylation of FAK in unsynchronized and mitotic cells. Among the four major phosphorylation sites, designated pS1-pS4, phosphorylation of pS1 (Ser722) is unchanged in unsynchronized and mitotic cells. In contrast, pS3 and pS4 (Ser843 and Ser910) exhibit increased phosphorylation during mitosis. In vitro peptide binding experiments provide evidence that phosphorylation of pS1 (Ser722) may play a role in modulating FAK binding to the SH3 domain of the adapter protein p130Cas.
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Eder, Lihi, Vinod Chandran, Fawnda Pellet, Sutha Shanmugarajah, Cheryl F. Rosen, Shelley B. Bull, and Dafna D. Gladman. "Human leucocyte antigen risk alleles for psoriatic arthritis among patients with psoriasis." Annals of the Rheumatic Diseases 71, no. 1 (September 6, 2011): 50–55. http://dx.doi.org/10.1136/ard.2011.155044.
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AimGenes that differentiate patients with psoriatic arthritis (PsA) from those with cutaneous psoriasis (PsC) may serve as markers for the development of PsA in patients with psoriasis. The authors aimed to identify human leucocyte antigen (HLA) alleles that are associated with the development of PsA in patients with psoriasis.Methods712 adult patients with PsA, 335 adult patients with PsC and 713 healthy controls were genotyped for HLA-A, HLA-B, HLA-C, HLA-DR and HLA-DQ alleles. Differences in allelic distributions for each of the HLA loci were compared using a likelihood ratio test. Logistic regression analysis of multiple loci was performed to account for linkage disequilibrium. Haplotype information was inferred using the expectation–maximisation algorithm (given HLA-C and HLA-B genotypes) and analysed similarly.ResultsThe following HLA alleles were found to be significantly associated with patients with PsA compared to patients with PsC in multivariate regression analysis: B*08 (OR 1.61, p=0.009), B*27 (OR 5.17, p<0.0001), B*38 (OR 1.65, p=0.026) and C*06 (OR 0.58, p=0.0002). HLA-B*27, HLA-B*38 and HLA-C*06 frequencies were also significantly higher in patients with PsA than in healthy controls (B*27: OR 3.05, p<0.0001; B*38: OR 5.9, p<0.0001; HLA-C*06: OR 1.71, p<0.0001). The following haplotypes were independently associated with PsA compared to PsC: HLA-B*18-C*07 (OR 10.1, p=0.004), HLA-B*27-C*01 (OR 41.1, p<0.0001), HLA-B*27-C*02 (OR 19.9, p<0.0001), HLA-B*38-C*12 (OR 2.9, p=0.01), HLA-B*08-C*07 (OR 2.6, p=0.004) and HLA-B*57-C*06 (OR 0.5, p=0.03).ConclusionsCertain HLA-B and HLA-C alleles confer susceptibility to PsA among patients with psoriasis and may be used to identify patients with PsC who may develop PsA.
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Polat, Erkan. "Investigation of influence of concrete material models on cyclic inelastic response of a concrete filled composite plate shear wall." Challenge Journal of Structural Mechanics 6, no. 2 (June 17, 2020): 91. http://dx.doi.org/10.20528/cjsmec.2020.02.006.
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A previously benchmarked finite element model of a previously tested composite plate shear wall-concrete filled (C-PSW/CF) was used to investigate the influence of three concrete material models on in-plane cyclic inelastic wall response, using LS-Dyna. The concrete material models considered were the Winfrith, KCC and CSCM, all available in LS-Dyna. Wall moment hysteresis, using the three concrete material models, were obtained and compared. Individual contribution of the steel and concrete to total base moment was investigated for each wall with the three concrete material models. The numerical results obtained using the KCC and CSCM were compared against the benchmarked results obtained using the Winfrith concrete material model. Moment contribution of the steel web and the steel boundary on total base moment of the steel part of the wall and moment contribution of the concrete web and concrete boundary on total base moment of the concrete part of the wall were individually investigated. The wall models with the KCC and CSCM concrete models were observed to cannot capture wall pinching which was captured by the Winfrith concrete model. The wall strength was overpredicted by the CSCM concrete model and predicted reasonably by the KCC concrete model. Average axial stress distribution of the infill concrete was obtained to investigate wall neutral axis and the maximum attained concrete strength using the three concrete models. Concrete axial stress distribution showed some level of confinement for the concrete models considered.
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Caranoni, C., P. Lampin, and C. Boulesteix. "Identification of two lead perovskites, Pb2ScTaO6 and Pb(Sc0.5Nb0.5)O3, by X-ray powder diffraction patterns." Powder Diffraction 8, no. 3 (September 1993): 191–93. http://dx.doi.org/10.1017/s0885715600018170.
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Substituting cations in materials with the formula Pb2B′B″O6 is more or less ordered on the B sites. High-quality single crystals of Pb2ScTaO6 (PST) and Pb(Sc0.5Nb0.5)O3 (PSN) were prepared from two thermal cycles. A stoichiometric mixture of the constituent oxides was prefired at up to 1000 °C, and then crystals were grown from a PbO–B2O3–PbF2 flux mixture, starting at a temperature of 1100 °C for PSN and 1200 °C for PST. At room temperature, X-ray examination showed that PSN had a perovskite structure with a cubic unit-cell and a refined parameter a = 4.080(1 ) Å, space group Pm3m and Z = 1, whereas PST formed a well-ordered superlattice with a = 8.136(1) Å, Z = 4 and space group Fm3m. In each case a fully indexed powder pattern is presented. The degree of order is estimated to be close to 80% for PST and less than 10% for PSN.
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Sekiya, Hiroshi, Maho Okada, Eiji Tamai, Toshi Shimamoto, Tadashi Shimamoto, and Hirofumi Nariya. "A Putative Amidase Endolysin Encoded by Clostridium perfringens St13 Exhibits Specific Lytic Activity and Synergizes with the Muramidase Endolysin Psm." Antibiotics 10, no. 3 (March 1, 2021): 245. http://dx.doi.org/10.3390/antibiotics10030245.
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Clostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. Potential treatments include phage-derived endolysins, a promising family of alternative antimicrobial agents. We surveyed the genome of the C. perfringens st13 strain and identified an endolysin gene, psa, in the phage remnant region. Psa has an N-terminal catalytic domain that is homologous to the amidase_2 domain, and a C-terminal domain of unknown function. psa and gene derivatives encoding various Psa subdomains were cloned and expressed in Escherichia coli as N-terminal histidine-tagged proteins. Purified His-tagged full-length Psa protein (Psa-his) showed C. perfringens-specific lytic activity in turbidity reduction assays. In addition, we demonstrated that the uncharacterized C-terminal domain has cell wall-binding activity. Furthermore, cell wall-binding measurements showed that Psa binding was highly specific to C. perfringens. These results indicated that Psa is an amidase endolysin that specifically lyses C. perfringens; the enzyme’s specificity is highly dependent on the binding of the C-terminal domain. Moreover, Psa was shown to have a synergistic effect with another C. perfringens-specific endolysin, Psm, which is a muramidase that cleaves peptidoglycan at a site distinct from that targeted by Psa. The combination of Psa and Psm may be effective in the treatment and prevention of C. perfringens infections.
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Babkova, M. I., and E. N. Otteva. "Subclinical psoriatic arthritis in a population of patients with psoriasis." Public health of the Far East Peer-reviewed scientific and practical journal 93, no. 3 (October 7, 2022): 15–22. http://dx.doi.org/10.33454/1728-1261-2022-3-15-22.
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Psoriasis (PsO) is a chronic immune-mediated disease, in the pathogenesis of which various risk factors and genetic predisposition play an important role. Psoriatic arthritis (arthropathic psoriasis, PsA) is a chronic inflammatory disease of the joints, spine, and entheses that can occur in patients with psoriasis. Every fourth patient with PsO of smooth skin develops psoriatic arthritis (PsA), which, if progressed and left untreated, can cause irreversible functional disorders, which necessitates early diagnosis of this disease. The aim of the study was to assess the frequency of detection of PsA in patients with PsO in the dermatovenerological department of the regional dermatovenerological dispensary using the mPEST questionnaire. The study involved 128 patients (73 men and 55 women, mean age 45 ± 13.69 years) with different clinical forms of PsO who received treatment in the adult dermatovenereology department No. 1 of the regional dispensary in the period from 2019 to 2021. The duration of the disease lasted an average of 6.1±2.76 years. Patients with PsO were divided into 3 groups according to the clinical manifestations of the skin process. The first group (n=96) included patients with an established clinical diagnosis of vulgar (common) psoriasis (PsO(c) without damage to the nail plates. The second group (n=24) was diagnosed with exudative psoriasis (PsO(e)). the third group (n=8) included patients with lesions of the skin and nail plates (PsO(o)). Based on mPEST≥3, the diagnosis of PsA was suspected in 34 (35.41%) patients diagnosed with PsO(c). In 10 (41.66%) patients of the second group with a diagnosis of PsO(e) and in 3 (37.5%) patients of the third. According to the results of the DLQI questionnaire, a moderate effect of the skin process on the quality of life was revealed in the first group of patients with PsO(c), which amounted to 10 points. In the second PsO(e) and third PsO(o) groups, a strong influence of the skin process on the quality of life was revealed - 18 and 11 points, respectively. The diagnosis of PsA in the first group with a diagnosis of PsO(c) according to the BASDAI questionnaire can be suspected in 85 (86.73%) patients, in group II - in 21 (87.5%) patients, in group III - PsA - in 7 (87.5%) %) of patients.
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Cao, Yajie, Runwen Ma, Ziyong Li, Xinyu Mao, Yinan Li, Yuxin Wu, Leping Wang, et al. "Broad-Spectrum Salmonella Phages PSE-D1 and PST-H1 Controls Salmonella in Foods." Viruses 14, no. 12 (November 27, 2022): 2647. http://dx.doi.org/10.3390/v14122647.
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Food contamination by Salmonella can lead to serious foodborne diseases that constantly threaten public health. Innovative and effective strategies are needed to control foodborne pathogenic contamination since the incidence of foodborne diseases has increased gradually. In the present study, two broad-spectrum phages named Salmonella phage PSE-D1 and Salmonella phage PST-H1 were isolated from sewage in China. Phages PSE-D1 and PST-H1 were obtained by enrichment with Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) CVCC1806 and Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) CVCC3384, respectively. They were able to lyse Salmonella, E. coli and K. pneumoniae and exhibited broad host range. Further study demonstrated that PSE-D1 and PST-H1 showed high pH and thermal tolerances. Phage PSE-D1 belongs to the Jiaodavirus genus, Tevenvirinae subfamily, while phage PST-H1 belongs to the Jerseyvirus genus, Guernseyvirinae subfamily according to morphology and phylogeny. The results of genome analysis showed that PSE-D1 and PST-H1 lack virulence and drug-resistance genes. The effects of PSE-D1 and PST-H1 on controlling S. Enteritidis CVCC1806 and S. Typhimurium CVCC3384 contamination in three kinds of foods (eggshells, sausages and milk) were further investigated, respectively. Our results showed that, compared to phage-free groups, PSE-D1 and PST-H1 inhibited the growth of their host strain significantly. A significant reduction of host bacteria titers (1.5 and 1.9 log10 CFU/sample, p < 0.001) on eggshells was observed under PSE-D1 and PST-H1 treatments, respectively. Furthermore, administration of PSE-D1 and PST-H1 decreased the counts of bacteria by 1.1 and 1.2 log10 CFU/cm2 (p < 0.001) in sausages as well as 1.5 and 1.8 log10 CFU/mL (p < 0.001) in milk, respectively. Interesting, the bacteriostasis efficacy of both phages exhibited more significantly at 4 °C than that at 28 °C in eggshells and milk and sausages. In sum, the purpose of our research was evaluating the counteracting effect of phage PSE-D1 and PST-H1 on the spread of Salmonella on contaminated foods products. Our results suggested that these two phage-based biocontrol treatments are promising strategies for controlling pathogenic Salmonella contaminated food.
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Reshetov, Yaroslav Yevgen'yevich, Anastasiya Aleksandrovna Ligacheva, Yelena Yur'yevna Avdeeva, Marina Grigor'yevna Danilets, Viktoriya Vladimirovna Golovchenko, Yevgeniya Sergeyevna Trofimova, Yekaterina Igorevna Gulina, et al. "POLYSACCHARIDES OF THREE SAUSSUREA DC SPECIES (S. CONTROVERSA, S. SALICIFOLIA, S. FROLOVII): ISOLATION, CHARACTERIZATION AND INFLUENCE ON NO-PRODUCING PROPERTIES OF MACROPHAGES." chemistry of plant raw material, no. 4 (December 27, 2019): 77–85. http://dx.doi.org/10.14258/jcprm.2019045483.
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Polysaccharides PS1 and PS2 with a yield 1–2% were obtained from the above-ground part of three species of Saussurea: S. controversa DC., S. salicifolia L. and S. frolovii Ledeb. sequential extraction with water at 25 and 70 °C. PS1 free from protein impurities. A protein that is not removed by the Sevag method is co-extracted with PS2. All polysaccharides contain residues of uronic acids, the highest content found in PS1 and PS2 from S. contrоversa. The molecular weights (Mw) of PS1 from S. controversa, S. salicifolia and S. frolovii were 448.13, 158.49, 64.03 kDa and PS2 – 101.82, 94.60, 225.42 kDa, respectively. Interspecific differences in the monosaccharide composition of polysaccharides were revealed. Galactose (Gal) and Arabinose (Ara) residues are major, and Rhamnose (Rha), Xylose (Xyl) and Mannose (Man) residues are minor components of the carbohydrate chains isolated by PS. PS1 S. salicifolia and S. frolovii and PS2 S. salicifolia do not contain endotoxins impurities and have a NO-activating effect on antigen-presenting cells (macrophages), significantly exceeding the effect of muramyl dipeptide.
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Yang, Albert-Jackson, Shinta Marito, John-Jackson Yang, Sunita Keshari, Chee-Ho Chew, Chien-Chung Chen, and Chun-Ming Huang. "A Microtube Array Membrane (MTAM) Encapsulated Live Fermenting Staphylococcus epidermidis as a Skin Probiotic Patch against Cutibacterium acnes." International Journal of Molecular Sciences 20, no. 1 (December 20, 2018): 14. http://dx.doi.org/10.3390/ijms20010014.
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Antibiotics without selectivity for acne treatment may destroy the beneficial microbes in the human microbiome that helps to fight Cutibacterium acnes (C. acnes), a bacterium associated with inflammatory acne vulgaris. Probiotic treatment by direct application of live Staphylococcus epidermidis (S. epidermidis) onto the open acne lesions may run the risk of bloodstream infections. Here, we fabricated the polysulfone microtube array membranes (PSF MTAM) to encapsulate probiotic S. epidermidis. We demonstrate that the application of the encapsulation of S. epidermidis in PSF MTAM enhanced the glycerol fermentation activities of S. epidermidis. To mimic the granulomatous type of acne inflammatory acne vulgaris, the ears of mice were injected intradermally with C. acnes to induce the secretion of macrophage inflammatory protein-2 (MIP-2), a murine counterpart of human interleukin (IL)-8. The C. acnes-injected mouse ears were covered with a PST MTAM encapsulated with or without S. epidermidis in the presence of glycerol. The application of S. epidermidis-encapsulated PST MTAM plus glycerol onto the C. acnes-injected mouse ears considerably reduced the growth of C. acnes and the production of MIP-2. Furthermore, no S. epidermidis leaked from PSF MTAM into mouse skin. The S. epidermidis-encapsulated PST MTAM functions as a probiotic acne patch.
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Saulnier, Luc, Jean-Marc Brillouet, and Michel Moutounet. "Nouvelles acquisitions structurales sur les substances pectiques de la pulpe de raisin." OENO One 22, no. 2 (June 30, 1988): 135. http://dx.doi.org/10.20870/oeno-one.1988.22.2.1262.
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<p style="text-align: justify;">Après une mise au point rapide sur les connaissances actuelles des polyosides pectiquesdans les végétaux, les résultats acquis sur la structure des substances pectiques de la pulpe deraisin sont exposés, avec un rappel de la méthodologie utilisée.</p><p style="text-align: justify;">Un matériel insoluble à l'alcool (MIA) a été préparé à partir de pulpe de raisin. Quatre fractionspectiques en ont été isolées après traitements successifs par l'eau (25°C; PSE), l'oxalate(25°C; PSOX, l'acide (HCI 0.05M, 80°C; PSH) et la soude (O,05M, 4°C; PSOH).</p><p style="text-align: justify;">Les PSE (35 p.100) et PSH (55 p. 100) représentent l'essentiel du matériel pectique extrait.Les PSE sont séparées par chromatographie d'échange d'ions en fractions neutre (PSEn~ 13 p. 100) et acide (PSEa ~ 87 p. 100). Les PSEa et les PSH sont constituées principalementd'acide galacturonique (PSEa 63 p. 100, PSH 53 p. 100), fortement estérifié par du méthanol(degré d'estérification : PSEa 77 p. 100, PSH 68 p. 100), tandis que des quantités faibles d'acideglucuronique sont détectées dans les PSEn (2 p. 100). Les oses neutres (PSEn 65 p. 100, PSEa28 p. 100, PSH 19 p. 100) sont principalement de l'arabinose et du galactose suivis dans unordre décroissant du rhamnose, glucose, xylose, mannose et fucose. Des protéines sont égaIementdétectées en association avec les polyosides.</p><p style="text-align: justify;">L'action d'une endopolygalacturonase et d'une endopectine-Iyase sur PSEa et PSH met enévidence des zones «lisses» homogalacturoniques dégradées par les enzymes et des zones«hérissées» rhamnogalacturoniques riches en chaînes latérales d'oses neutres, insensibles àl'attaque enzymatique. Le traitement du MIA par une endopectine-Iyase solubilise un matérielpectique (ZH-MIA) riche en oses neutres (56 p. 100) particulièrement en arabinose, et contenantde l'acide galacturonique (23 p. 100) et des protéines (11 p. 100).</p><p style="text-align: justify;">La perméthylation associée à l'hydrolyse spécifique de l'arabinose par une α-L-arabinofuranosidaseet à la RMN du <sup>13</sup>C a permis d'établir la structure des chaînes latérales. Des (1 → 3) / (1 → 6) arabinogalactanes, où l'arabinose est essentiellement sous forme terminale non réductrice, dominent dans les ZH-PSE, tout comme dans les PSEn confirmant leur caractère d'arabinogalactane-protéine. En revanche ces structures ainsi que les (1 → 4) arabinogalactanes sont minoritaires dans les ZH-PSH et ZH-MIA où les structures de type (1 → 5) arabinanes et rhamnogalacturonanes sont prédominants.</p><p style="text-align: justify;">+++</p><p style="text-align: justify;">After a brief review of available knowledges on plant pectic polysaccharides, structural data on pectic substances from the pulp or grape berries and related analytical techniques are reported.</p><p style="text-align: justify;">An alcohol insoluble residue (MIA) was prepared from pulp of grape berries, which was sequentially extracted with water (25°C), oxalate (25°C), acid (0,05N HCI, 80°C) and sodium hydroxide (0,05N 4°C) yielding four pectic fractions, respectively, PSE, PSOX, PSH and PSOH. PSE (35 p. 100) and PSH (55 p. 100) represented the main part of extracted pectic material.</p><p style="text-align: justify;">PSE were fractionated by ion-exchange chromatography into neutral (PSEn ~ 13 p. 100) and acidic (PSEa ~ 87 p. 100) fractions. PSEa and PSH were constituted mainly of galacturonic acid (PSEa 63 p. 100, PSH 53 p. 100) highly methylesterified (esterification degree : PSEa 77 p. 100; PSH 68 p. 100), whereas PSEn contained minute amounts of glucuronic acid (2 p. 100). Neutral sugars (PSEn 65 p. 100, PSEa 28 p. 100, PSH 19 p. 100) were mainly arabinose and galactose followed by decreasing amounts of rhamnose, xylose, glucose, mannose and fucose. Proteins were also detected along with the polysaccharides.</p><p style="text-align: justify;">Degradation of PSEa and PSH by endopolygalacturonase and endopectin-lyase evidenced «smooth» homogalacturonic areas sensitive to enzymatic degradation and «hairy» rhamnogalacturonic zones highly substituted by neutral sugar side-chains and resistant to enzyme action. Treatment of MIA With endopectinlyase released pectic material (ZH-MIA) rich in neutral sugars (56 p. 100), especially arabinose, and containing galacturonic acid (23 p. 100) and proteins (11 P. 100).</p><p style="text-align: justify;">Structure of neutral sugar side-chains was investigated using methylation analysis associated with specific hydrolysis of arabinose residues with an α-L-arabinofuranosidase, and <sup>13</sup>C NMR spectroscopy. ZH-PSE exhibited a structure of 3,6 -linked arabinogalactan substitued by monomeric terminal arabinose. Similar structures were detected in PSEn which relates them to arabino-3,6-galactan-proteins. Conversely PSH or ZH-MIA showed mainly arabinan-like and rhamnogalacturonan structures associated with minor proportions of 3,6- and 4-linked arabino-galactans.</p>
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Izci, M., S. Kanyilmaz Turgut, O. Akan, I. Topal, and O. Kuru. "POS0146 PERIPHERAL NEUROPATHY IN PSORIASIS AND PSORIATIC ARTHRITIS: A SINGLE BLIND, CONTROLLED, ELECTROPHYSIOLOGICAL STUDY." Annals of the Rheumatic Diseases 80, Suppl 1 (May 19, 2021): 286.1–286. http://dx.doi.org/10.1136/annrheumdis-2021-eular.1258.
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Background:It is well known that both psoriasis (PsO) and psoriatic arthritis (PsA) causes morbidity and disability in patients’ lives. Additional pathologies such as peripheral neuropathy (PN) may interfere with disease specific symptoms and may increase the burden of disease. Neuropathic complaints of patients with PsO and PsA are observed during patients’ follow-up (1,2). However, the presence of PN in PsO and PsA is not well defined in clinical studies and clinicians might be neglecting this issue.Objectives:Our aim was to investigate the presence of PN by electrophysiological methods and to examine its relationship with neuropathic pain and with treatments in patients with PsO and PsA.Methods:38 patients with PsA, 36 patients with PsO and 37 age&sex-matched healthy volunteers were enrolled into this prospective, single-blind study. Disease activity was assessed with Psoriasis Area Severity Index (PASI) for patients with PsO and with Disease Activity in Psoriatic Arthritis (DAPSA) score for patients with PsA. Leeds Assessment of Neuropathic Symptoms and Signs (LANSS) score was used to evaluate neuropathic pain. All participants were electrophysiologically examined in Electromyography (EMG) Laboratory by an experienced neurologist blinded for study groups.Results:There were no significant differences on patients age, sex and body mass index among 3 study groups (p>0.05). However, disease duration was significantly longer in PsO group compared to PsA group (median 19.1[1-43] years in PsO vs median 7.5[2-29] years in PsA; p<0.01). PN was detected in 7 patients (19.44%) in the PsO group, 14 patients (34.21%) in the PsA group, and in 1 volunteer (2.7%) in the control group (PsO vs control: p<0.05; PsA vs control: p<0.001, PsO vs PsA: p>0.05) (Table 1). Among patients with PsA, 1 patient had ulnar neuropathy and 1 patient had both carpal tunnel syndrome (CTS) and sensory polyneuropathy. All other PNs detected electrophysiologically were CTS. Neuropathic pain was present in 11 patients (30.56%), 20 patients (52.63%) and in 2 volunteers (5.41%) in PsO, PsA and control groups consecutively (PsO vs control: p<0.05; PsA vs control: p<0.001, PsO vs PsA: p>0.05). PN did not show any significant correlation with disease activity or treatments including biological agents.Table 1.Results of peripheral neuropathy and neuropathic pain in control group and patients with PsO and PsAControl Group (n=37)PsO Group (n=36)PsA Group (n=38)p^p+p&CTS1 (%2.70)7 (%19.44)13* (%34.21)0.028a<0.001a0.153aUlnar Neuropathy001 (%2.63)Polyneuropathy001* (%2.63)Total PN1 (%2.70)7 (%19.44)14 (%36.64)0.028a<0.001a0.097aLANSS Score5 (0:13)6.50 (0:16)13 (3:19)0.170b<0.001b0.004bAccording to LANSSlikely neuropathic pain2 (%5.41)11 (%30.56)20 (%52.63)0.005 a<0.001a0.054a*: Both polyneuropathy and CTS were detected in one patient in the PsA group; Data are given as n (%) and median (minimum: maximum); a: Chi-Square Test, b: Kruskal Wallis Test; p^ value: control vs PsO, p+ value: control vs PsA, p& value: PsO vs PsAConclusion:To our knowledge, this is the first study to investigate simultaneously the presence of neuropathic pain and PN by electrophysiological methods in patients with PsO and PsA. We observed PN and neuropathic pain high in patients with PsO&PsA and among them CTS appears as the most prominent neuropathy. Clinicians should keep this in mind during routine care of patients with PsO and PsA, which may improve patient-centred care. Large, prospective studies are needed to understand the characteristics of these neuropathies and its prognosis.References:[1]Rifbjerg-Madsen S, Christensen AW, Christensen R, Hetland ML, Bliddal H, Kristensen LE, Danneskiold-Samsøe B, Amris K (2017) Pain and pain mechanisms in patients with inflammatory arthritis: a Danish nationwide cross-sectional DANBIO registry survey. PLoS One 12(7):e0180014.[2]Ljosaa TM, Mork C, Stubhaug A, et al. Skin pain and skin discomfort is associated with quality of life in patients with psoriasis. J Eur Acad Dermatol Venereol. 2012;26:29-35.Disclosure of Interests:None declared
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Yin, Le Bin, Yong Liu, De Yong Zhang, and Song Bai Zhang. "Isolation and Characterization of Rhodopseudomonas sp. S9-1 Capable of Degrading Pyrazosulfuron-Ethyl." Advanced Materials Research 356-360 (October 2011): 1152–63. http://dx.doi.org/10.4028/www.scientific.net/amr.356-360.1152.
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A bacterial strain S9-1capable of degrading sulfonylurea herbicide pyrazosulfuron-ethyl (PSE) was isolated from contaminated soil through the enrichment incubation method. Based on morphology, colony and cultural properties, physiological and biochemical characteristics, living-cell absorption spectra, internal photosynthetic membrane, and phylogenetics of its 16S rRNA gene sequence, S9-1was preliminarily identified as belonging to the genus Rhodopseudomonas, a group of photosynthetic bacteria (PSB). The effects of PSE concentration, pH, and temperature on biodegradation were examined. The degradation rate was found to decrease with increasing PSE concentration. Optimal growth pH and temperature were found to be 7.0 and 30°C, respectively. The strain was able to degrade 47.51% of PSE at a concentration of 100 mg ml-1after 7 days of incubation at 30°C and could tolerate 800 mg ml-1PSE. S9-1was also able to completely co-metabolically transform 100 mg ml-1PSE at 30°C, pH 7.0, and 7500 lux in 15 days. As the concentration of PSE increased, the degradation process took longer to complete. The fragment encoding acetolactate synthase (ALS) gene from S9-1was cloned and sequenced. Comparison of deduced amino acid sequences was implemented, and the conserved sites were analyzed. To our knowledge, this is the first report of PSB in PSE biodegradation. These results highlight the potential of this bacterium as a detoxifying agent for use with PSE-contaminated soil and wastewater.
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De Merlier, D., A. Chandelier, and M. Cavelier. "First Report of Phytophthora ramorum on Viburnum bodnantense in Belgium." Plant Disease 87, no. 2 (February 2003): 203. http://dx.doi.org/10.1094/pdis.2003.87.2.203c.
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In the past decade, a new Phytophthora species inducing shoot canker on Rhododendron and dieback of Viburnum has been observed in Europe, mainly in Germany and the Netherlands, and California. This new pathogen has been named Phytophthora ramorum (3). In May 2002, a diseased Viburnum plant (Viburnum bodnantense) from the Plant Protection Service (Ministry of Agriculture, Belgium) was submitted to our laboratory for diagnosis. Symptoms included wilting, leaves turning from green to brown, discolored vascular tissues, and root necrosis. The plant came from a Belgian ornamental nursery that obtained supplies of stock plants from the Netherlands. Pieces of necrotic root tissue were excised, surface-disinfected, and transferred aseptically to a Phytophthora selective medium. P. ramorum was identified based on morphological characteristics, including the production of numerous, thin-walled chlamydospores (25 to 70 µm in diameter, average 43 µm) and deciduous, semi-papillate sporangia arranged in clusters. Radial growth after 6 days at 20°C on V8 juice agar was 2.8 mm per day. Random amplified microsatellite markers (RAMS) (2) from the total genomic DNA of the Belgian strain (CBS 110901) were similar to those of P. ramorum reference strains (CBS 101330, CBS 101332, and CBS 101554). Using PCR primers specific for P. ramorum, the identification was confirmed by W. A. Man in't Veld (Plantenziektenkundige Dienst, Wageningen, the Netherlands) (1). A pathogenicity test was carried out on three sterile cuttings of Rhododendron catawbiense (3). Brown lesions were observed on the inoculated cuttings after 6 to 7 days. None of the three uninoculated cuttings showed symptoms of infection. P. ramorum was reisolated from lesion margins on the inoculated cuttings. To our knowledge, this is the first report of the fungus from Belgium. Since our initial observation, we have found P. ramorum in other Belgian nurseries on R. yakusimanum. References: (1) M. Garbelotto et al. US For. Ser. Gen. Tech. Rep. PSW-GRT. 184:765, 2002. (2) J. Hantula et al. Mycol. Res. 101:565, 1997. (3) S. Werres et al. Mycol. Res. 105:1155, 2001.
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Eder, Lihi, Fatima Abji, Cheryl F. Rosen, Vinod Chandran, Richard J. Cook, and Dafna D. Gladman. "The Association of HLA-class I Genes and the Extent of Atherosclerotic Plaques in Patients with Psoriatic Disease." Journal of Rheumatology 43, no. 10 (September 1, 2016): 1844–51. http://dx.doi.org/10.3899/jrheum.151469.
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Objective.To investigate the association between HLA susceptibility and disease severity markers and the extent of atherosclerosis in patients with psoriatic disease.Methods.White patients with psoriatic arthritis (PsA) and psoriasis without PsA (PsC) were recruited. An ultrasound of the carotid arteries was performed and the size of each atherosclerotic plaque was measured. The resulting score, the total plaque area (TPA), represented the extent of atherosclerosis. HLA genotyping was performed using sequence-specific oligonucleotide probes. The association between 10 HLA susceptibility and severity markers of PsC and PsA and the severity of atherosclerosis was assessed by ordinal logistic regression models adjusted for age, sex, and cardiovascular (CV) risk factors.Results.The study involved 411 patients (273 PsA, 138 PsC). Of them, 61.8% had at least 1 atherosclerotic plaque. HLA-B*13:02 and HLA-C*06:02 were associated with more severe atherosclerosis (age- and sex-adjusted OR 2.31, 95% CI 1.23–4.32 and OR 1.68, 95% CI 1.12–2.52, respectively). HLA-B*38:01 was associated with less severe atherosclerosis (OR 0.49, 95% CI 0.28–0.86). These associations remained statistically significant after adjusting for CV risk factors. Higher levels of erythrocyte sedimentation rate (ESR) were associated with more severe atherosclerosis (age- and sex-adjusted OR 1.33, p = 0.02). HLA-B*13:02–positive (p = 0.01) as well as HLA-C*06:02–positive (p = 0.008) patients had higher levels of ESR over time.Conclusion.HLA-C*06:02 and B*13:02 alleles are associated with a higher burden of atherosclerosis in patients with psoriatic disease. This association may be mediated by a higher level of systemic inflammation.
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Gola, Agnieszka, Andrea Bernardi, Gianfranco Pasut, and Witold Musiał. "The Influence of Initiator Concentration on Selected Properties of Thermosensitive Poly(Acrylamide-co-2-Acrylamido-2-Methyl-1-Propanesulfonic Acid) Microparticles." Polymers 13, no. 7 (March 24, 2021): 996. http://dx.doi.org/10.3390/polym13070996.
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Thermosensitive polymers PS1–PS5 were synthesized via the surfactant free precipitation polymerization (SFPP) using 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPSA), and potassium persulfate (KPS) at 70 °C in aqueous environment. The effect of KPS concentrations on particle size and lower critical temperature solution (LCST) was examined by dynamic light scattering (DLS). The conductivity in the course of the synthesis and during cooling were investigated. The structural studies were performed by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), H nuclear magnetic resonance (1H NMR), thermogravimetric analysis (TGA/DTA) and powder X-ray diffraction (PXRD). ATR-FTIR, 1H NMR and PXRD data confirmed the polymeric nature of the material. TGA/DTA curves demonstrated thermal stability up to approx. 160 °C. The effect of temperature on the hydrodynamic diameter (HD) and zeta potential (ZP) were evaluated by dynamic light scattering (DLS) and electrophoretic mobility (EM) in 18–45 °C range. The LCST values were between 30 and 34 °C. HD and polydispersity index (PDI) of aqueous dispersions of the synthesized polymers PS1–PS5 at 18 °C were found to be 226 ± 35 nm (PDI = 0.42 ± 0.04), 299 ± 145 nm (PDI = 0.49 ± 0.29), 389 ± 39 nm (PDI = 0.28 ± 0.07), 584 ± 75 nm (PDI = 0.44 ± 0.06), and 271 ± 50.00 nm (PDI = 0.26 ± 0.14), respectively. At 18 °C the ZPs of synthesized polymers suspensions were −13.14 ± 2.85 mV, −19.52 ± 2.86 mV, −7.73 ± 2.76 mV, −7.99 ± 1.70 mV, and −9.05 ± 2.60 mV for PS1–PS5, respectively. We found that the initiator concentration influences the physicochemical properties of products including the size of polymeric particles and the LCST.
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SUGA, Kei, Takami TOMIYAMA, Hiroshi MORI, and Kimio AKAGAWA. "Syntaxin 5 interacts with presenilin holoproteins, but not with their N- or C-terminal fragments, and affects β-amyloid peptide production." Biochemical Journal 381, no. 3 (July 27, 2004): 619–28. http://dx.doi.org/10.1042/bj20040618.
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Mutations in presenilins 1 and 2 (PS1 and PS2) account for the majority of cases of early-onset familial Alzheimer's disease. However, the trafficking and interaction of PSs with other proteins in the early secretory pathways are poorly understood. Using co-immunoprecipitation, we found that PS bound to Syx5 (syntaxin 5), which is a target-soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor involved in endoplasmic reticulum (ER)–Golgi vesicular transport in vivo. Syx5 interacted only with the full-length PS holoproteins and not with the naturally occurring N- or C-terminal fragments. The PS holoproteins co-immunoprecipitated with the mutant Syx5, which localized to the ER and Golgi compartments, despite the substitution of the transmembrane region with that of syntaxin 1A. In contrast, the transmembrane deletion mutant that localized to the cytosol, but not to the ER or Golgi compartments, did not co-immunoprecipitate the PS holoproteins. The PS1 variant linked to familial Alzheimer's disease (PS1ΔE9), lacking the region that contains the endoproteolytic cleavage site in the cytoplasmic loop, showed markedly decreased binding to Syx5. Immunofluorescence and sucrose-density-gradient fractionation analyses showed that the full-length PS holoproteins co-localized with Syx5 to the ER and cis-Golgi compartments. Furthermore, Syx5 overexpression resulted in the accumulation of PS holoproteins and the β-amyloid precursor protein, and reduced the secretion of the Aβ (amyloid β) peptide in COS-7 cells. In summary, these results indicate that Syx5 binds to full-length PSs and affects the processing and trafficking of β-amyloid precursor protein in the early secretory compartments.
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Aktas, Aynur, Declan Walsh, Lisa A. Rybicki, and Anne Fitz. "Symptom clusters and demographic characteristics in advanced cancer." Journal of Clinical Oncology 31, no. 15_suppl (May 20, 2013): 9638. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.9638.
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9638 Background: Little is known about demographic variations in cancer symptom clusters (SC). Our objective was to determine whether SC are associated with age, gender, race, performance status (PS), or primary cancer site. Methods: Symptoms from 1000 advanced cancer patients referred to a palliative medicine program were recorded prospectively. Among 922 patients with complete symptom data, hierarchical cluster analysis identified 7 SC. A SC was considered present if the patient had ≥50% of the symptoms in the cluster. Comparisons were made between patients with and without each cluster using the chi-square test (age <65 vs. ≥65 years; gender female (F) vs. male (M); race Caucasian (C) vs. African American (AA); 10 primary site groups (PSG), or Wilcoxon rank sum test (ECOG PS 0-4). A p value <0.05 indicated statistical significance. Results: 83% of patients were C, 52% ≥65 years, 56% M, and 55% had ECOG PS 3-4 Most common PSG were lung (25%), genitourinary (18%), and gastrointestinal (GI) (11%). Fatigue/anorexia-cachexia cluster was associated with race (58% AA vs. 68% C, p=0.032) and PSG (range 47% melanoma to 83% pancreas, p=0.012); Neuropsychological cluster was associated with older age (29% ≥65 vs. 39% <65, p<0.001) and race (22% AA vs. 36% C, p=0.001). Upper GI cluster was associated with female gender (16% M vs. 22% F, p=0.035) and PSG (range 8% Head & Neck to 32% pancreas, p=0.035). Nausea/Vomiting cluster was associated with younger age (35% ≥65 vs. 43% <65, p=0.010) and female gender (33% M vs. 47% F, p<0.001). Aerodigestive cluster was associated with male gender (36% F vs. 44% M, p=0.010) and PSG (range 24% pancreas to 58% Head & Neck, p<0.001). Debility cluster was associated with race (33% AA vs. 44% C, p=0.016) and poor PS (range 17% PS0 to 54% PS4, p<0.001). Pain cluster was associated with younger age (88% ≥65 vs. 92% <65, p=0.028). Conclusions: We identified 7 SC whose prevalence were influenced by age, gender, race, PS, or primary cancer site. This supports the clinical relevance of the cluster concept in palliative and supportive care. Demographic characteristics may warrant different clinical approaches to patient care. Identification of these differences may help develop more effective cancer treatment and management strategies.
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Pereverzina, N., L. Kruglova, T. Korotaeva, and A. Lila. "POS1102 THE PREVALENCE OF INFLAMMATORY BACK PAIN IN PATIENTS WITH SKIN PSORIASIS WITHOUT PSORIATIC ARTHRITIS. DATA FROM DERMATOLOGICAL REAL-WORLD SETTING." Annals of the Rheumatic Diseases 81, Suppl 1 (May 23, 2022): 879.1–879. http://dx.doi.org/10.1136/annrheumdis-2022-eular.5046.
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BackgroundPsoriasis (PsO) is an inflammatory disease associated with psoriatic arthritis (PsA). PsA affects peripheral and axial joints1,2. Psoriasis can precede the onset of PsA by approximately 3 to 8 years, axial involvement often undiagnosed3. There are limited data about the prevalence of inflammatory back pain (IBP) in PsO patients (pts) without clinical symptoms of PsA4.ObjectivesTo study the prevalence of IBP in PsO pts without PsA symptomsMethods108 PsO pts without PsA over a 10-month period (March 2021 to December 2021) were incluided. Participants filled out a questionnaire on IBP. Additionaly the prevalence of stiffness in the back and neck was estimated. IBP was defined as an affirmative answer to the question ‘Did you suffer from low back pain for ≥3 months?’ and IBP criteria was based on criteria ASAS (the ASsessment in Ankylosing Spondylitis) and was confirmed if at least 3 out of the following 4 criteria were present: (a) onset before age 40, (b) insidious onset, (c) improvement with exercise, (d) associated with morning stiffnessResultsIt was found that more than 40% of patients complained of pain or stiffness in the back. Stiffness and pain in the neck were observed in every fourth patient on average. Of those patients who complained of pain in the back or neck, 37% noted that they had pain at rest (when they get up in the morning or lie down for a long time), and 21.3% had inflammatory pain at night (when patients are forced to woke up, they needed to do exercises) (Table 1). It was found that the risks of developing spondylitis with neck pain, neck stiffness, back pain, back stiffness were higher in 4,549 times (95% CI [1,720; 12,031]), in 19,444 times (95% CI [6,480; 58,343]) 15 times (95% CI [4.646; 48.427]) and 34.857 times (95% CI [7.583; 160.230]), respectively.ConclusionIBP was found in more than 40% PsO patients. Every fourth PsO patients complained of stiffness and pain in the neck. The presence of these symptoms significantly increased the risk of developing PsA with spondylitis. The presence of IBP should be taken into account for early diagnosis of PsA in the dermatological practice.References[1]Gladman DD. Axial disease in psoriatic arthritis. Curr Rheumatol Rep. 2007 Dec;9(6):455-60. doi: 10.1007/s11926-007-0074-2.[2]Chandran V, Barrett J, Schentag CT, Farewell VT, Gladman DD. Axial psoriatic arthritis: update on a longterm prospective study. J Rheumatol. 2009 Dec;36(12):2744-50. doi: 10.3899/jrheum.090412.[3]Queiro R, Belzunegui J, González C, De DJ, Sarasqueta C, Torre JC, Figueroa M. Clinically asymptomatic axial disease in psoriatic spondyloarthropathy. A retrospective study. Clin Rheumatol. 2002 Feb;21(1):10-3. doi: 10.1007/s100670200003.[4]Gottlieb AB, Merola JF. Axial psoriatic arthritis: An update for dermatologists. J Am Acad Dermatol. 2021 Jan;84(1):92-101. doi: 10.1016/j.jaad.2020.05.089.Disclosure of InterestsNatalia Pereverzina: None declared, Larisa Kruglova: None declared, Tatiana Korotaeva Speakers bureau: Pfizer, MSD, AbbVie, Novartis-Sandoz, JSC Biocad, Janssen, UCB, Alexander Lila: None declared
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Eder, Lihi, Fatima Abji, Cheryl F. Rosen, Vinod Chandran, and Dafna D. Gladman. "The Association Between Obesity and Clinical Features of Psoriatic Arthritis: A Case-control Study." Journal of Rheumatology 44, no. 4 (February 15, 2017): 437–43. http://dx.doi.org/10.3899/jrheum.160532.
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Objective.To assess whether obesity is associated with distinct psoriatic arthritis (PsA) features and whether it interacts with PsA HLA susceptibility alleles.Methods.Patients with early PsA were compared with patients with psoriasis without arthritis (PsC). The primary predictor was the body mass index (BMI) at the first visit to the clinic. The clinical features across 3 BMI groups were compared by linear trend test and Cochrane-Armitage trend test. The interaction between BMI and HLA risk alleles for psoriatic disease (HLA-B*27, B*3901, B*3801, B*0801, B*4402, B*4403, and C*0602) were assessed using logistic regression analysis.Results.There were 314 patients with early PsA, and 498 patients with PsC were analyzed. Obesity was more frequent in patients with PsA compared with PsC (OR 1.77; p = 0.002). Higher BMI was associated with older age at onset of PsA (p < 0.0001) and psoriasis (p = 0.009). The frequency of HLA-B*27 was higher in patients with normal weight compared with those with higher BMI (p = 0.002). A significant interaction was found for the combined effect of HLA-B*27 and obesity in logistic regression analysis (p = 0.036). In patients who were HLA-B*27–negative, the association between obesity and PsA was statistically significant (OR 2.39; p < 0.001), but obesity was less frequent in patients with PsA who were HLA-B*27–positive.Conclusion.Obesity is linked with late-onset psoriasis and PsA, while normal weight is associated with the presence of the HLA-B*27 allele and an earlier onset of the disease. These results highlight the differential risk factors that may drive the inflammatory process in psoriatic disease.
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Petrie, Benjamin K., Tudor Sturzoiu, Julie Shulman, Saleh Abbas, Hesham Masoud, Jose Rafael Romero, Tatiana Filina, et al. "Questionnaire and Portable Sleep Test Screening of Sleep Disordered Breathing in Acute Stroke and TIA." Journal of Clinical Medicine 10, no. 16 (August 13, 2021): 3568. http://dx.doi.org/10.3390/jcm10163568.
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Sleep disordered breathing (SDB) is highly prevalent, but frequently unrecognized among stroke patients. Polysomnography (PSG) is difficult to perform soon after a stroke. We evaluated the use of screening questionnaires and portable sleep testing (PST) for patients with acute stroke, subarachnoid hemorrhage, or transient ischemic attack to expedite SDB diagnosis and management. We performed a single-center retrospective analysis of a quality improvement study on SDB screening of consecutive daytime, weekday, adult admissions to a stroke unit. We excluded patients who were unable to communicate and lacked available family members. Patients were screened with the Epworth Sleepiness Scale, Berlin Questionnaire, and STOP-BANG Questionnaire and underwent overnight PST and/or outpatient PSG. The 4-item STOP Questionnaire was derived from STOP-BANG for a secondary analysis. We compared the sensitivity and specificity of the questionnaires for the diagnosis of at least mild SDB (apnea hypopnea index (AHI) ≥5) on PST and correlated AHI measurements between PST and PSG using the Spearman correlation. Out of sixty-eight patients included in the study, 54 (80%) were diagnosed with SDB. Only one (1.5%) had a previous SDB diagnosis. Thirty-three patients completed all questionnaires and a PST. The STOP-BANG questionnaire had the highest sensitivity for at least mild SDB (0.81, 95% CI (confidence interval): 0.65–0.92) but a low specificity (0.33, 95% CI 0.10, 0.65). The discrimination of all questionnaires was overall poor (C statistic range 0.502–0.640). There was a strong correlation (r = 0.71) between the AHI results estimated using PST and outpatient PSG among 28 patients. The 4-item STOP Questionnaire was the easiest to administer and had a comparable or better sensitivity than the other questionnaires. Inpatient PSTs were useful for screening in the acute setting to facilitate an early diagnosis of SDB and to establish further outpatient evaluations with sleep medicine.
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Kowal, Anthony S., Thomas Scheibel, and Susan L. Lindquist. "Observation of Amyloid-Like Fibers of the Sup35 Protein from Saccharomyces Cerevisiae." Microscopy and Microanalysis 6, S2 (August 2000): 664–65. http://dx.doi.org/10.1017/s1431927600035819.
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In the yeast Saccharomyces cerevisiae, [PST] acts as an epigenetic modifier of translation termination efficiency. [PSI+] can be passed through generations of yeast cells via changes in protein conformation rather than changes in DNA or RNA, and has thus been referred to as a yeast prion. The [PSI+] determinant is the Sup35 protein. Sup35 can exist in two states - soluble and insoluble. Soluble Sup35 functions in translation termination, but when insoluble, stop codons are read through, resulting in incorrect protein products.Sup35 is composed of three distinct domains, N, M, and C. The N region is rich in glutamine and asparagine and is required for the [PST] phenotype to exist. M is a highly charged domain, and no specific function has been assigned to it. C is essential in yeast, as it is responsible for translation termination. The insoluble form of Sup35 has characteristics reminiscent of other prion proteins - in vitro it binds to the dye Congo Red and it exhibits apple green birefringence in polarized light.
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Chimenti, M. S., M. Galluzzo, M. Talamonti, A. Campanati, C. Bonifati, P. Bruni, G. Caldarola, et al. "AB0915 GUSELKUMAB REAL WORD DATA: EFFICACY AND SAFETY IN A COHORT OF 69 PATIENTS WITH PSORIATIC ARTHRITIS." Annals of the Rheumatic Diseases 81, Suppl 1 (May 23, 2022): 1587.1–1587. http://dx.doi.org/10.1136/annrheumdis-2022-eular.4327.
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BackgroundPsoriasis (PSO) is a systemic immune-mediated disorder, characterized by inflammation skin and joint manifestations: it is known that up to 30% of PsO patients develop PsA.PsO and PsA share common etiopathogenetic pathways, as IL-23/IL-17 axis. In Italy, Guselkumab (GUS), a selective IL-23 inhibitor, was approved in 2018 for the treatment of PsO, and recently it was also approved for PsA therapy. Today, there are few “real-world” studies regarding the use of GUS in PsA patients.ObjectivesA multicenter Italian study group of dermatologists and rheumatologists aimed at evaluate GUS efficacy and safety in patients with concomitant PsO and PsA in real word setting on both skin and joint domains.MethodsAn observational retrospective, multicentric study was performed in 69 PsO patients with a confirmed diagnosis of moderate to severe PsA. PASI and DLQI were used for the evaluation of skin response, and the number swollen/tender joints, presence or absence of dactylitis or enthesitis or axial involvement and painVAS were evaluated for the articular and periarticular efficacy. These analyses were performed at baseline T0 (beginning of the therapy), T1 (12 weeks) and T2 (24 weeks).Results38/69 patients (55,1%) presented oligoarthritis, 31/69 (44,9%) showed polyarthritis, none of the patients had enthesitis or axial involvement. Moreover, co-morbidities were diagnosed: hypertension (52,2%), hypercholesterolemia (34,8%), Hypertriglyceridemia (29%), diabetes (24,6%), obesity (23,2%), HIV-positive (20,3%), psychiatric disorders (17,4%), cardiopathies (15,9%), inflammatory bowel disease (7,3%), Latent Tubercolosis (4,4%), Chronic B-Hepatitis (2,9%), Chronic C-Hepatitis (1,5%). In all these patients, skin and joint responses were evaluated at week 12 and week 24.Concerning skin efficacy, PASI 90 was achieved at week 24. Concerning Joint response: painVAS progressively improved till T2, tender joint count decreased in patients with oligo and polyarthritis at T1 and maintained at T2, while swollen joint count decreased in polyarthritis patients at T1 and maintained at T2. In oligo-arthritis patients, this parameter was not improved. The number of dactylitis did not decrease during the period of study (see Table 1). No safety concerns were reported in this population.ConclusionEfficacy and safety of GUS was confirmed in this study group of PsO patients with concomitant PsA and several comorbidities in a real-life setting.Disclosure of InterestsNone declared
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Choi-Kwon, Smi, Mihye Ko, Sang-Eun Jun, Juhan Kim, Kyung-Hee Cho, Hyun-Wook Nah, Hasup Song, and Jong S. Kim. "Post-Stroke Fatigue May Be Associated with the Promoter Region of a Monoamine Oxidase A Gene Polymorphism." Cerebrovascular Diseases 43, no. 1-2 (November 19, 2016): 54–58. http://dx.doi.org/10.1159/000450894.
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Background: Post-stroke fatigue (PSF) is a common sequela of stroke. Despite reports of serotonergic involvement in the etiology of PSF, the potential contribution of serotonergic genes in the development of PSF needs to be investigated. Methods: A total of 373 patients, who experienced ischemic stroke for PSF, were evaluated 3 months after the stroke. PSF was assessed using the Fatigue Severity Scale. The genomic DNA collected and stored in a -70°C freezer was genotyped for 6 polymorphisms in genes associated with serotonin synthesis (tryptophan hydroxylase 1 (TPH1) A218C, TPH2 rs10879355, and TPH2 rs4641528), transport (the promoter region of the serotonin transporter protein), and catabolism (the 30-bp functional variable number tandem repeat) polymorphism in the promoter region of monoamine oxidase A (MAO-A). Results: Among the 373 patients, 164 (44%) had PSF. All patients were ethnic Koreans. Of the 6 polymorphisms examined, only one marker, that is, low-activity MAO-A was associated with PSF (p < 0.05) in female patients. Multiple logistic regression analyses showed that post-stroke depression (PSD; 95% CI 1.561-14.323, p = 0.006) and low MAO-A activity (95% CI 0.166-0.722, p = 0.005) were factors associated with PSF in female patients, whereas only PSD (95% CI 5.511-65.269, p = 0.000) was associated with PSF in male patients. Conclusions: Our findings suggest that PSF may be associated with a genetic polymorphism involving MAO-A, at least in female stroke patients.
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Zabotti, A., G. De Marco, L. Gossec, X. Baraliakos, J. Emmel, D. Aletaha, A. Iagnocco, J. S. Smolen, and D. Mcgonagle. "POS1079 CLINICAL CHARACTERIZATION OF PRODROMAL AND VERY EARLY PSORIATIC ARTHRITIS: A SYSTEMATIC LITERATURE REVIEW FOR THE DEFINITION OF CLINICAL AND IMAGING SUSPICIOUS FEATURES FOR PROGRESSION TO PSORIATIC ARTHRITIS." Annals of the Rheumatic Diseases 81, Suppl 1 (May 23, 2022): 865.1–865. http://dx.doi.org/10.1136/annrheumdis-2022-eular.2340.
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BackgroundThe transition from psoriasis (PsO) to psoriatic arthritis (PsA) is a focus of considerable scientific interest: is it possible to target pre-PsA and very early PsA, in particular for physiopathology and drug studies? Recently, a EULAR taskforce has been set up in this area (1).ObjectivesTo characterize the prodromal and the very early PsA through a systematic literature review (SLR).MethodsA SLR explored MEDLINE, EMBASE and CENTRAL, up to 22 October 2021. The objective was to identify the symptoms, objective signs, lab tests, imaging features and other characteristics of patients later diagnosed as “new onset” PsA in two key populations: 1) patients with PsO and 2) patients with early undifferentiated arthritis (UA). Studies of adult patients published in English were included, if they reported characteristics of pre-PsA or new onset PsA patients, and data were extracted by 2 readers. Meta-analysis was not done due to data heterogeneity (PsA classification criteria, outcome measures and length of observation). Results are reported semi-quantitatively.ResultsOf 31449 references, 22 studies were included of which 12 were prospective; 7 retrospective and 3 cross-sectional. Eighteen studies reported on patients with PsO (n=95828) later diagnosed as PsA (n=2136) with a mean duration of follow up of 5.2 (±3.9) years. Seven out of 18 (38.8%) studies were informative regarding the clinical features of the new onset PsA. Four studies on early UA patients (n=492) later diagnosed as PsA (n=49) were included. Progression to PsA was associated with the presence of musculoskeletal (MSK) complaints (mainly joint tenderness) and the presence of subclinical MSK inflammation detected by imaging. Peripheral oligo-arthritis was the prevalent clinical presentation of new onset PsA.ConclusionAs expected, joint pain and imaging evidence of MSK inflammation were associated with PsA development in PsO patients. The SLR highlights the lack of robust evidence regarding data associated with the development of PsA. More prospective studies focusing on transition from PsO to PsA are needed.Table 1.FeaturesTransition from PsO to PsA (n = 18 studies)Transition from UA to PsA (n = 4 studies)Clinical characterization of New Onset PsA (n = 5 studies)Patient reported symptomsVAS pain+++NAEntheseal pain+NAMorning stiffness+NAFatigue+NAHAQ more compromised++NAArthralgia+++NAClinical examinationJoint tenderness+++++++Swelling joints++++Entheseal tendernessMajor domain of pattern presentationPeripheral arthritis (more frequent)+++PolyarthritisMono-oligoarthritis++Inflammatory marker(s)CRP++ImagingMSK inflammation detected by imaging++++Radiographic evidence of specific damage++Legend:PsO = psoriasis (affecting skin); PsA = psoriatic arthritis; UA = undifferentiated inflammatory arthritis; VAS = visual-analogue scale; NA = not applicable; HAQ = health assessment questionnaire; CRP = C-reactive protein; MSK = musculoskeletal+ = 1 study for positive association; ++ = 2 studies for positive association; +++ >= 3 studies for positive associationReferences[1]https://www.eular.org/ongoing_initiatives.cfmDisclosure of InterestsAlen Zabotti Speakers bureau: Amgen, Lilly, Janssen, Novartis, UCB, Paid instructor for: Amgen, Janssen, Grant/research support from: Novartis, Gabriele De Marco: None declared, Laure Gossec Speakers bureau: AbbVie, Amgen, BMS, Galapagos, Gilead, GSK, Janssen, Lilly, Novartis, Pfizer, Samsung Bioepis, Sanofi-Aventis, UCB, Grant/research support from: Amgen, Galapagos, Lilly, Pfizer, Sandoz, Xenofon Baraliakos: None declared, Jenny Emmel: None declared, Daniel Aletaha Speakers bureau: Abbvie, Amgen, Lilly, Janssen, Merck, Novartis, Pfizer, Roche, Sandoz, Grant/research support from: Abbvie, Amgen, Lilly, Novartis, Roche, SoBi, Sanofi, Annamaria Iagnocco: None declared, Josef S. Smolen: None declared, Dennis McGonagle Speakers bureau: Janssen, Lilly, UCB, Abbvie, Pfizer, Celgene, Grant/research support from: Janssen
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Smolik-Utlaut, S. M. "Dosage requirements of Ultrabithorax and bithoraxoid in the determination of segment identity in Drosophila melanogaster." Genetics 124, no. 2 (February 1, 1990): 357–66. http://dx.doi.org/10.1093/genetics/124.2.357.
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Abstract The wild-type Ultrabithorax (Ubx) and bithoraxoid (bxd) functions are primarily responsible for establishing the identity of parasegment 6 (PS6) in the Drosophila embryo and thus the identity of the posterior compartment of the third thoracic segment (pT3) and the anterior compartment of the first abdominal segment (aA1) in the adult. The experiments described were designed to test the ability of an increased dosage of Ubx+ and bxd+ to affect the transformation of PS5 toward PS6. The results are consistent with the ideas that (1) multiple copies of Ubx+ and bxd+ cause some cells within PS5 to take on the characteristics of PS6 cells but do not cause an overall parasegmental transformation of PS5 toward PS6, (2) cellular identity depends not only on the activity of Ubx+ but on its concentration as well, and (3) that an interaction between Ubx+ and the wild-type Antennapedia (Antp) gene establishes segmental identity in pT2. In the first instar larvae carrying eight copies of Ubx+ and bxd+ the fine hairs of the T3 setal belt are transformed toward the hook-like structures of the A1 setal belt. Other structures within this segment are unaffected. In the adult, the haltere is reduced in size. The transformation of pT2 cells (wing) toward pT3 cells (haltere) is seen in adults carrying eight doses of wild type Ubx and bxd by decreasing the amount of the bithorax complex (BX-C) regulator Polycomb (Pc). However, the transformation of the T3 setal belt is not enhanced in the larvae of these animals. The interaction between the genes of the Antennapedia complex (ANT-C) and the Ubx+ and bxd+ functions in pT2 is dosage sensitive only when the animals carry one copy of Pc. In these animals, the transformation of wing toward haltere is significantly enhanced.
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Diana Abdul Majid, Noor, Dzulkarnain Zaini, and Azmi Mohd Shariff. "Managing Employee Participation based on OSHA’s Process Safety Management Requirements." International Journal of Engineering & Technology 7, no. 3.26 (August 14, 2018): 13. http://dx.doi.org/10.14419/ijet.v7i3.26.17453.
Full textAbstract:
A report published on 25 April 2016 stated that OSHA has issued 14 serious violations after an investigation conducted in a variety of industries including manufacturing, water treatment and oil and gas that violated OSHA's Process Safety Management (PSM) standard. One of the violations is related to the 29 CFR 1910.119(c)(2) which is employers did not consult with employees and their representatives on the conduct and development of the OSHA’s PSM elements which are process safety information (PSI), process hazard analyses (PHA), training (TNG), mechanical integrity (MI), management of change (MOC), pre-start up safety review (PSSR), contractors (CON), incident investigations (II), emergency planning and response (EPR), compliance audit (CA) and trade secret (TS). 29 CFR1910.119(c)(2) is one of the requirements in the OSHA’s PSM employee participation element. However, companies are still struggling to comply with this regulation due to unclear coverage and the implementation method for achieving compliance. Thus, this paper presents a framework and work-aid tool developed based on OSHA’s PSM 29 CFR 1910.119(c) which are helpful to the industries as they provide structured technique to plan and implement an employee's participation management system to achieve compliance in implementing the OSHA PSM employee participation element.
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Liang, Ziwei, Enyu Zhang, Ling Duan, Nathaniel Weygant, Guangyu An, Bin Hu, and Jiannan Yao. "Establishment of a Competing Risk Nomogram in Patients with Pulmonary Sarcomatoid Carcinoma." Technology in Cancer Research & Treatment 21 (January 2022): 153303382110689. http://dx.doi.org/10.1177/15330338211068960.
Full textAbstract:
Background and aim: Pulmonary sarcomatoid carcinoma (PSC) is a rare subtype of nonsmall cell lung cancer with a poor prognosis. This study aimed to analyze the clinicopathological characteristics and survival outcomes among patients with PSC, lung squamous cell cancer (SCC), and lung adenocarcinoma (LAC), and to construct a competing risk nomogram for patients with PSC. Method: Data of 3 groups of patients diagnosed with PSC, SCC, or LAC from the surveillance, epidemiology, and end results (SEER) database between 1988 and 2015 were retrospectively reviewed. A 1:1 propensity score matching (PSM) analysis was used to balance the baseline data of patients. Independent risk factors associated with survival outcomes were screened by the least absolute shrinkage and selection operator and further determined by univariate and multivariate Cox proportional risk regression analyses. The overall survival (OS) of patients was evaluated by Kaplan–Meier analysis and compared with a log-rank test. The cumulative incidence function was used to estimate the 5-year probabilities of the cancer-specific mortality of PSC. A nomogram was constructed to illustrate the competing risk model to predict the 3- and 5-year OS, and corresponding concordance indexes (C-indexes) and calibration curves were used to assess and validate the competing risk nomogram. Results: A total of 2285 patients with PSC were included in this study. Compared with SCC and LAC patients, the Kaplan–Meier analysis showed that patients with PSC had a worse prognosis, with a median survival of 5 months (95% confidence interval [CI]: 5-6 months) and a 5-year OS rate of 15.3% (95% CI: 13.9%-16.9%). Similar outcomes were demonstrated after 1:1 PSM. Moreover, the competing risk model showed that age, T stage, M stage, tumor size, lymph node ratio (LNR), surgery, and chemotherapy were associated with PSC-specific mortality. The 5-year C-index of the nomogram was 0.718. Calibration curves illustrated that the nomogram was well-validated and had great accuracy. Conclusions: Patients with PSC had a worse survival outcome compared with SCC or LAC patients. Age, T stage, M stage, tumor size, LNR, surgery, and chemotherapy were associated with PSC-specific mortality. The competing risk nomogram displayed excellent discrimination in predicting PSC-specific mortality.