Dissertations / Theses on the topic 'C - hetero'

This thesis concerned about the Ru-catalyzed C-H functionalizations on the synthesis of 2-arylindole unit, silylation of heteroarenes and preparation of aryne precursor. In the first project, we developed the Ru-catalyzed C2-H arylation of N-(2-pyrimidyl) indoles and pyrroles with nucleophilic arylboronic acids under oxidative conditions. Wide variety of arylboronic acids afforded the desired product in excellent yield regardless of the substituents or functional group electronic nature. Electron-rich heteroarenes are well suited for this method than electron-poor heteroarenes. Halides such as bromide and iodide also survived, further derivatisation of the halide is shown by Heck alkenylation. In order to find catalytic on-cycle intermediate extensive mechanistic experiments have been carried out by preparing presumed ruthenacyclic complexes and C-H/D exchange reactions. It suggested that para-cymene ligand is not present in the catalytic on-cycle intermediate and we suspect that metalation occurs with electrophilic ruthenium center via SEAr mechanism. In the second project, we developed the Ru-catalyzed silylation of gramine, tryptamine and their congeners using silanes as coupling partner. The transformation worked well with many different silanes. Regarding directing group, nitrogen atom containing directing groups are more favoured than the oxygen containing directing groups. Wide range of gramines and tryptamines also yielded the desired product in poor to excellent yield. At higher temperature, albeit in low yield, undirected silylation occurred. In order to get some insights about the reaction pathway of the silylation C-H/D exchange experiments were performed, and it revealed the possibility of C4-H activation of gramines by an electron rich metal- Si-H/D experiments showed Si-H activation by Ru is easy. In the final project, we presented the closely related aryne precursors from arylboronic acids via Ru-catalyzed C-H silylation of arylboronates and their selective oxidation. Worthy of note, the aryne capture products obtained from arylboronic acids in a single purification.

Afin d'expliquer le contexte de mon travail de recherche, j'ai résumé dans le premier chapitre des informations mécanistiques générales sur l'arylation des liaisons C-H catalysée par le palladium et j'ai détaillé certains travaux sur l'arylation directe en relation avec mes travaux. Mes objectifs étaient d'étudier la réactivité de nouvelles unités synthétiques permettant l'accès direct à des composés bi-(hétéro)aryls ou à des dérivés du styrène. Ensuite, dans les chapitres 2 à 6, j'ai résumé mes travaux de recherche. J'ai étudié l’arylation directes en C2 du Methoxsalen par des chlorures de benzènesulfonyle, ainsi que la diarylation en C2,C3 en utilisant des bromures d'aryle catalysée par le palladium. Ces résultats sont résumés dans le chapitre 2. Ensuite, j'ai montré que la réaction d'arylation directe catalysée par le palladium permet d'accéder facilement à des dérivés de la Ticlopidine arylés en position C5 du cycle thiényle en une seule étape. Ces résultats sont décrits dans le chapitre 3. Dans le chapitre 4, nous avons étudié la réactivité du Diflufénican qui contient un cycle 1,3- difluorobenzène et une unité pyridine en utilisant la catalyse au ruthénium et au palladium. Dans des conditions appropriées, deux liaisons C-H différentes du Diflufénican peuvent être arylées. Dans le chapitre 5, j'ai utilisé différentes sources d’aryle pour fonctionnaliser les positions C10 et C11 des dibenzo[b,f]azépines. Grâce à ces réactions, une grande diversité de groupes fonctionnels a été introduite sur les dérivés de dibenzo[b,f]azépines. Enfin, dans le chapitre 6, j’ai décrit la première méthode permettant de préparer des dérivés de la Cyproheptadine C10-arylés<br>In order to explain the background of my research work, in the first chapter, I summarized general mechanistic information on palladium-catalyzed C-H bond arylation and detailed some literature on direct arylation related to my research work. My objectives were to study the reactivity of new synthetic units allowing the straightforward access to bi-(hetero)aryls compounds or styrene derivatives using aryl halides or benzenesulfonyl chloride derivatives as the aryl-sources. Then, in the chapters 2-6, I summarized my research work. I studied the regiocontrolled palladium-catalyzed direct C2-arylations of Methoxsalen using benzenesulfonyl chlorides and C2,C3-diarylations using aryl bromides as the aryl sources. These results are summarized in the chapter 2. Then, I found that Pd-catalyzed direct arylation reaction allows the easy access to Ticlopidine derivatives arylated at the C5-position of the thienyl ring in one step. These results are reported in the chapter 3. In the chapter 4, we studied the reactivity of Diflufenican which contains a 1,3-difluorobenzene ring and a pyridine unit using Ru and Pd catalysis. Under appropriate conditions, two different C-H bonds of Diflufenican could be arylated. In the chapter 5, I employed different aryl sources to functionalize the C10- and C11-positions of dibenzo[b,f]azepines, and obtained asymmetric products. Through these reactions, a wide diversity of functional group were introduced on the dibenzo[b,f]azepine derivatives. Finally, in the Chapter 6, I report the first method allowing to prepare C10-arylated Cyproheptadine derivatives

Au cours de cette thèse, nous nous sommes intéressés à l'activation de liaisons sp² et sp³ C-H catalysée par le palladium pour la préparation d'(hétéro)aryl-aryles et de biaryles. Cette méthode est considérée comme attractive pour l'environnement par rapport aux méthodes classiques, tels que Suzuki, Heck, ou Negishi. Tout d'abord, nous avons décrit que la C2-arylation directe de benzothiophènes peut être effectuée par un catalyseur du palladium en l'absence de ligand phosphine avec une grande sélectivité. Nous avons également démontré qu'il est possible d'activer les positions C2 et C5 de pyrroles pour accéder en une seule étape a des 2,5-diarylpyrroles. Des 2,5-diarylpyrroles non-symétriques ont été formés par arylation séquentielle en C2 suivie par une arylation en C5. Nous avons également étudié la réactivité de polychlorobenzenes pour l'activation de liaisons C-H catalysé au palladium. Nous avons finalement étudié l'activation sp² et sp³ sélective catalysé au palladium de liaisons C-H du guaiazulene. La sélectivité de la réaction dépend du solvant et de la base : C2-arylation (KOAc en éthylbenzène), C3-arylation (KOAc dans le DMAc) et C4-Me arylation (CsOAc/K₂CO₃ dans le DMAc). Grâce à cette méthode, une liaison sp³ C-H peu réactive a été activée<br>During this thesis, we were interested in the sp² and sp³ C-H bond activation catalyzed by palladium catalysts for the preparation of (hetero)aryl-aryls and biaryls. This method is considered as cost effective and environmentally attractive compared to the classical couplings such as Suzuki, Heck, or Negishi. First we described the palladium-catalyzed direct C2-arylation of benzothiophene in the absence of phosphine ligand with high selectivity. We also demonstrated that it is possible to active both C2 and C5 C-H bonds for access to 2,5-diarylated compounds in one step, and also to non-symmetrically substituted 2,5-diarylpyrroles via sequential C2 arylation followed by C5 arylation. We also studied the reactivity of polychlorobenzenes via palladium-catalyzed C-H activation. We finally examined the palladium-catalysed selective sp² and sp³ C-H bond activation of guaiazulene. The selectivity depends on the solvent and base: sp² C2-arylation (KOAc in ethylbenzene), sp² C3-arylation (KOAc in DMAc) and sp³ C4-Me arylation (CsOAc/K₂CO₃ in DMAc). Through this method, a challenging sp³ C-H bond was activated

Septinas compreendem uma conservada família de proteínas de ligação a nucleotídeo de guanina e formação de heterofilamentos. Em termos estruturais, elas possuem uma organização comum: um domínio GTPase central, uma região N-terminal e um domínio C-terminal, este último é predito para formar estruturas em coiled coil. Atualmente, o heterocomplexo de septinas humanas (SEPT2/SEPT6/SEPT7) mais bem caracterizado revela a importância do domínio GTPase na formação do filamento, todavia a ausência de densidade eletrônica para os domínios C-terminais faz com que sua função permaneça obscura. Estudos com septinas de mamíferos, e de outros organismos como C. elegans e S. cerevisea sugerem que alguns grupos de septinas (por exemplo, II e IV em mamíferos) interagem através de seus domínios C-terminais, e estes poderiam atuar de modo determinante para a montagem correta do filamento. Assim, o presente projeto objetivou estudar a afinidade homo/heterotípicas para os domínios C-terminais das septinas humanas dos grupos II (SEPT6C/8C/10C/11C) e IV (SEPT7C), investigando se esses domínios contribuem para preferência das septinas interagirem com proteínas de grupos distintos durante a formação do heterofilamento. Os domínios C-terminais foram expressos em E. coli e purificados. Foram conduzidos estudos de ultracentrifugação analítica e espectropolarimetria de dicroísmo circular, que permitiram identificar maior afinidade e estabilidade da associação heterotípica comparada à homotípica. Foram obtidas constantes de dissociação aparente para homodímeros em torno de baixo &micro;M, enquanto que para heterodímeros os dados já existentes no grupo revelaram constante de dissociação na ordem de nM. Para entender os fatores no nível atômico responsáveis pela significativa predileção na interação entre os domínios C-terminais dos grupos II e IV foram realizados estudos utilizando modelagem e análise das sequências primárias. As análises sugerem a presença de um alto número de resíduos carregados na posição a do coiled coil como responsável pela seletividade. Consequentemente, o heterodímero seria favorecido em virtude do menor efeito repulsivo proveniente do intercalamento dos resíduos carregados em a. Desse modo, os resultados indicaram a atuação decisiva ou cooperativa dos domínios C-terminais na organização preferencial das septinas durante a formação do filamento, favorecendo a interface NC entre septinas dos grupos II e IV.<br>Septins comprise a conserved protein family that binds guanidine nucleotide and forms heterofilaments. In structural terms they have a common organization: a central GTPase domain, a N-terminal domain and a C-terminal domain, this last one is predicted to form coiled coil structures. Currently, the human septin heterocomplex best characterized (SEPT2/SEPT6/SEPT7) reveals the importance of the GTPase domain in filament assembly, however the absence of electron density for the C-terminal domains makes its function still unknown. Studies with mammals septins, and of others organisms like C. elegans and S. cerevisea suggests that some septins groups (e.g. II e IV in mammals) interact via its C-terminal domains and this could act in a determinative way to correct filament assembly. In this way, this project aimed to study the homo/heterotypical affinity for the C-terminal domains of human septins belonging to groups II (SEPT6C/8C/10C/11C) e IV (SEPT7C), investigating whether this domain contributes with the preference of septins to interact with proteins of different groups during assembly of the heterofilament. The C-terminal domains were expressed in E. coli and purificated. It was carried out studies using analytical ultracentrifugation and circular dichroism spectropolarimetry tecniques which allowed identification of major affinity and stability in the heterotypical association compared to homotypical. It was measured apparent dissociation constants for homodimers of low &micro;M range while for heterodimers our group\'s data shows dissociation constants in the nM range. To understand at atomic level the factors responsible for this significant preference in the C-terminal domains interaction between groups II and IV was performed molecular modelling studies and analysis of the primary sequence. These analysis suggests the presence of a high number of charged residues in position a of the coiled coil as responsible for selectivity. Consequently, the heterodimer would be therefore favoured because of the minor repulsive effect coming from the staggered of charged residues in a. Thus, these results indicate the crucial or cooperative action of C-terminal domains in preferential organization of septins during filament assembly, favouring the NC interface between septins of groups II and IV.

This thesis describes the synthetic studies performed utilizing tridentate Schiff base Cr(III) complex catalysts towards the total synthesis of ()-lasonolide A resulting in the completion of synthetic efforts towards the C17-C25 segment. Further summarization is also given on completion of the C7-C16 segment and an overview of future directions toward to the total synthesis of ()-lasonolide A. Both tetrahydropyrans C7-C11 and C19-C23 were to be constructed utilising an asymmetric hetero-Diels–Alder protocol involving Jacobsen’s hetero-Diels–Alder catalyst providing a means to generate a number of the desired stereocentres simultaneously in each ring system, followed by novel macrolide formation using ruthenium catalysts for metathesis olefination and Alder-ene chemistry. Jacobsen's hetero-Diels–Alder catalyst has also been utilised to generate C-disacharide linked precursors under diastereolective control in an effort to generate stereospecific 13 linked disaccharides.<br>Thesis (PhD Doctorate)<br>Doctor of Philosophy (PhD)<br>School of Biomolecular and Physical Sciences<br>Science, Environment, Engineering and Technology<br>Full Text

Se estudia la influencia del dopaje y de la temperatura sobre las propiedades físicas de hetero-estructuras hexagonales principalmente basadas en carbono y nitruro de boro tanto uni- y bi-dimensionales, este estudio se divide en tres partes. Por un lado, se estudia el efecto del dopaje de nitruro de boro en nanotubos de carbono armchair (6, 6) (hetero-nanotubos de nitruro de boro y carbono) sobre las propiedades del transporte cuántico de fonones y electrones, variando patrones de distribución y concentración de dopaje. Por otro lado, se estudia la forma como afecta la temperatura del sustrato sobre las propiedades del transporte térmico clásico en hetero-nanocintas de grafeno/nitruro de boro hexagonal y nitruro de boro hexagonal/grafeno, e influenciados por la asimetría estructural. Análogamente, también se estudia el efecto de la temperatura media y bias en hetero-nanocintas de grafeno/X (X=nitruro de boro hexagonal, carburo de silicio hexagonal, y grafeno hidrogenado), afectados por dos tipos de interfaces (zigzag y armchair). Por último, se estudia las propiedades electrónicas del proceso de adsorción de la molécula del Formaldehído (CH2O) sobre el sustrato de nitruro de boro hexagonal hidrogenado. Para ello, la metodología empleada en estos trabajos consistió en el uso de tres métodos computacionales; las funciones de Green fuera del equilibrio (NEGF) en combinación con el método de la teoría del funcional de la densidad basado en tight-binding (DFTB), dinámica molecular fuera del equilibrio (NEMD), y cálculos de la teoría del funcional de la densidad, respectivamente. Por un lado, los resultados respecto al efecto del dopaje sobre el transporte cuántico muestran que al aumentar la concentración del dopaje en los hetero-nanotubos de nitruro de boro y carbono armchair (6, 6), los coeficiente de transmisión fonónico y electrónico se reducen, este comportamiento ocurre a frecuencias altas y cerca de los bordes de la banda de valencia y conducción, respectivamente. Los patrones de distribución de dopaje helicoidal y horizontal permiten un transporte de fonones adecuados cuantificados en una alta conductancia de fonones a 300K. Todos los hetero-nanotubos de nitruro de boro y carbono armchair (6, 6) poseen un comportamiento semiconductor a diferencia del nanotubo de carbono armchair (6, 6) con un bandgap modulable respecto a la concentración de dopaje de nitruro de boro. Por otra parte, los resultados del efecto de la temperatura del sustrato sobre el transporte térmico clásico en las hetero-nanocintas muestran que existe una influencia que incrementa la rectificación térmica (>30 %) a temperaturas bajas (>300K) para el sustrato de Si(100), esto debido al acople fuerte tipo Van der waals entre el sustrato y la hetero-nanocinta de nitruro de boro hexagonal/grafeno. Además, la asimetría estructural tipo-T genera una rectificación térmica en sistemas puros y hetero-nanocintas de grafeno/nitruro de boro hexagonal y nitruro de boro hexagonal/grafeno, este efecto está relacionado con los cambios en el grado de localización de los modos vibracionales de frecuencias altas . Sin embargo, la resistencia térmica interfacial y la rectificación térmica se reducen con la temperatura media en las XIV hetero-nanocintas de grafeno/X, independientemente de la interface armchair o zigzag, debido a que el flujo térmico en direcciones opuestas llegan a ser similares a altas temperaturas. El efecto de la temperatura bias, en la curva J − α, determina que el fujo térmico tiene una preferencia marcada en la dirección de X al grafeno, observando el fenomeno de la resistencia térmica diferencial negativa en los sistemas de grafeno/nitruro de boro hexagonal y grafeno/grafano. Por último, la trayectoria de mínima energía, la densidad de estados total y la densidad de estados proyectada del proceso de adsorción muestran que la molécula formaldehído, CH2O, se quimisorbe sobre el sustrato de hBN hidrogenado con una energía de adsorción de 1.42eV mediante vacancias de hidrógeno en el átomo de B, donde también la distribución de densidad de espín y la transferencia de carga presentan evidencias de la existencia de una reacción en cadena de moléculas CH2O sobre el sustrato de nitruro de boro hexagonal.<br>Consejo Nacional de Ciencia y Tecnología (Perú). Fondo Nacional de Desarrollo Científico y Tecnológico (Fondecyt)<br>Tesis

Ce travail concerne les transistors bipolaires à hétérogène TBH SiGe. En particulier, l'auto-échauffement des transistors unitaires et le couplage thermique avec leurs plus proches voisins périphériques sont caractérisés et modélisés. La rétroaction électrothermique intra- et inter-transistor est largement étudiée. En outre, l’impact des effets thermiques sur la performance de deux circuits analogiques est évalué. L'effet d'autoéchauffement est évalué par des mesures à basse fréquence et des mesures impulsionnelles DC et AC. L'auto-échauffement est diminué de manière significative en utilisant des petites largeurs d'impulsion. Ainsi la dépendance fréquentielle de l’autoéchauffementa été étudiée en utilisant les paramètres H et Y. De nouvelles structures de test ont été fabriqués pour mesurer l'effet de couplage. Les facteurs de couplage thermique ont été extraits à partir de mesures ainsi que par simulations thermiques 3D. Les résultats montrent que le couplage des dispositifs intra est très prononcé. Un nouvel élément du modèle de résistance thermique récursive ainsi que le modèle de couplage thermique a été inclus dans un simulateur de circuit commercial. Une simulation transitoire entièrement couplée d'un oscillateur en anneau de 218 transistors a été effectuée. Ainsi, un retard de porte record de 1.65ps est démontré. À la connaissance des auteurs, c'est le résultat le plus rapide pour une technologie bipolaire. Le rendement thermique d'un amplificateur de puissance à 60GHz réalisé avec un réseau multi-transistor ou avec un transistor à plusieurs doigts est évalué. La performance électrique du transistor multidoigt est dégradée en raison de l'effet de couplage thermique important entre les doigts de l'émetteur. Un bon accord est constaté entre les mesures et les simulations des circuits en utilisant des modèles de transistors avec le réseau de couplage thermique. Enfin, les perspectives sur l'utilisation des résultats sont données<br>The power generate by modern silicon germanium (SiGe) heterojunction bipolar transistors (HBTs) can produce large thermal gradients across the silicon substrate. The device opering temperature modifies model parameters and can significantly affect circuit operation. This work characterizes and models self-heating and thermal coupling in SiGe HBTs. The self-heating effect is evaluated with low frequency and pulsed measurements. A novel pulse measurement system is presented that allows isothermal DC and RF measurements with 100ns pulses. Electrothermal intra- and inter-device feedback is extensively studied and the impact on the performance of two analog circuits is evaluated. Novel test structures are designed and fabricated to measure thermal coupling between single transistors (inter-device) as well as between the emitter stripes of a multi-finger transistor (intra-device). Thermal coupling factors are extracted from measurements and from 3D thermal simulations. Thermally coupled simulations of a ring oscillator (RO) with 218 transistors and of a 60GHz power amplifier (PA) are carried out. Current mode logic (CML) ROs are designed and measured. Layout optimizations lead to record gate delay of 1.65ps. The thermal performance of a 60GHz power amplifier is compared when realized with a multi-transistor array (MTA) and with a multi-finger trasistor (MFT). Finally, perspectives of this work within a CAD based circuit design environment are discussed

Ce travail de thèse présente une évaluation approfondie des différentes techniques de mesure transitoire et dynamique pour l’évaluation du comportement électro-thermique des transistors bipolaires à hétérojonctions HBT SiGe:C de la technologie BiCMOS et des transistors Métal-Oxyde-Semiconducteur à effet de champ (MOSFET) de la technologie CMOS 45nm. En particulier, je propose une nouvelle approche pour caractériser avec précision le régime transitoire d'auto-échauffement, basée sur des mesures impulsionelles. La méthodologie a été vérifiée par des mesures statiques à différentes températures ambiantes, des mesures de paramètres S à basses fréquences et des simulations thermiques transitoires. Des simulations thermiques par éléments finis (TCAD) en trois dimensions ont été réalisées sur les transistors HBTs de la technologie submicroniques SiGe: C BiCMOS. Cette technologie est caractérisée par une fréquence de transition fT de 230 GHz et une fréquence maximum d’oscillation fMAX de 290 GHz. Par ailleurs, cette étude a été réalisée sur les différentes géométries de transistor. Une évaluation complète des mécanismes d'auto-échauffement dans les domaines temporels et fréquentiels a été réalisée. Une expression généralisée de l'impédance thermique dans le domaine fréquentiel a été formulée et a été utilisé pour extraire cette impédance en deçà de la fréquence de coupure thermique. Les paramètres thermiques ont été extraits par des simulations compactes grâce au modèle compact de transistors auquel un modèle électro-thermique a été ajouté via le nœud de température. Les travaux théoriques développés à ce jour pour la modélisation d'impédance thermique ont été vérifiés avec nos résultats expérimentaux. Il a été montré que, le réseau thermique classique utilisant un pôle unique n'est pas suffisant pour modéliser avec précision le comportement thermique transitoire et donc qu’un réseau plus complexe doit être utilisé. Ainsi, nous validons expérimentalement pour la première fois, le modèle distribué électrothermique de l'impédance thermique utilisant un réseau nodal récursif. Le réseau récursif a été vérifié par des simulations TCAD, ainsi que par des mesures et celles ci se sont révélées en excellent accord. Par conséquent, un modèle électro-thermique multi-géométries basé sur le réseau récursif a été développé. Le modèle a été vérifié par des simulations numériques ainsi que par des mesures de paramètre S à basse fréquence et finalement la conformité est excellente quelque soit la géométrie des dispositifs<br>An extensive evaluation of different techniques for transient and dynamic electro-thermal behavior of microwave SiGe:C BiCMOS hetero-junction bipolar transistors (HBT) and nano-scale metal-oxide-semiconductor field-effect transistors (MOSFETs) have been presented. In particular, new and simple approach to accurately characterize the transient self-heating effect, based on pulse measurements, is demonstrated. The methodology is verified by static measurements at different ambient temperatures, s-parameter measurements at low frequency region and transient thermal simulations. Three dimensional thermal TCAD simulations are performed on different geometries of the submicron SiGe:C BiCMOS HBTs with fT and fmax of 230 GHz and 290 GHz, respectively. A comprehensive evaluation of device self-heating in time and frequency domain has been investigated. A generalized expression for the frequency-domain thermal impedance has been formulated and that is used to extract device thermal impedance below thermal cut-off frequency. The thermal parameters are extracted through transistor compact model simulations connecting electro-thermal network at temperature node. Theoretical works for thermal impedance modeling using different networks, developed until date, have been verified with our experimental results. We report for the first time the experimental verification of the distributed electrothermal model for thermal impedance using a nodal and recursive network. It has been shown that, the conventional single pole thermal network is not sufficient to accurately model the transient thermal spreading behavior and therefore a recursive network needs to be used. Recursive network is verified with device simulations as well as measurements and found to be in excellent agreement. Therefore, finally a scalable electro-thermal model using this recursive network is developed. The scalability has been verified through numerical simulations as well as by low frequency measurements and excellent conformity has been found in for various device geometries

Submitted by PPG Engenharia e Tecnologia de Materiais (engenharia.pg.materiais@pucrs.br) on 2018-12-03T15:52:09Z No. of bitstreams: 1 Dissertacao DANIELA MAFFI RODRIGUES.pdf: 1641622 bytes, checksum: 7201a3a551a602c84d3776d0f02f7399 (MD5)<br>Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-12-05T13:35:48Z (GMT) No. of bitstreams: 1 Dissertacao DANIELA MAFFI RODRIGUES.pdf: 1641622 bytes, checksum: 7201a3a551a602c84d3776d0f02f7399 (MD5)<br>Made available in DSpace on 2018-12-05T13:50:46Z (GMT). No. of bitstreams: 1 Dissertacao DANIELA MAFFI RODRIGUES.pdf: 1641622 bytes, checksum: 7201a3a551a602c84d3776d0f02f7399 (MD5) Previous issue date: 2018-07-27<br>Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES<br>Carbon capture and storage (CCS) and carbon capture and utilisation(CCU) technologies has been pointed out as possibilities to mitigate the effects caused by the massiverelease of CO2 into the atmosphere. The use of CO2 in cycloaddition reactions toepoxides obtaining cyclic carbonates is an interesting possibility to reduce CO2emission. Cyclic carbonates are products of great applicability as solvents in the chemical industry and are used as raw material for a wide range of products. Meanwhile, the use of CO2 for a cycloaddition reaction requires a large amount of energy being necessary the use of catalysts in order to optimize such reactions. Poly (ionic liquids) (PIL) are alternative catalysts due its selective, recyclability and conversion. In this work, cellulosic poly(ionic liquids) (CPIL) obtained from rice husk were testedas heterogeneous catalyst. Cellulose extraction was carried out by chemical method. A chemical modification of the cellulose fibers was carried out by the treatment with citric acid and functionalization with 3 mmol of the ionic liquids BMIM Cl, TBAB, TBPB and BMPYRR to form CPIL-BMIM, CPIL-TBA, CPIL-TBP and CPIL-BMPYRR), without addition of solvents. Cyclo addition reactions were carried out with propylene and styrene oxides with different CO2 pressures(25, 30 and 40 bar) and temperatures (90, 110 and 120 ?C) for 6 h. The obtained product was characterized by gas chromatography (GC), Fourier Transform Infrared Spectroscopy(FTIR)and Nuclear Magnetic Resonance (1H NMR). The results showed better yields for CPIL-TBP and CPIL-TBA, whose values were 53.2% and 39% respectively for propylene carbonate and 67.3% for styrene carbonate using CPIL-TBP. When the ZnBr2 cocatalyst was used, there was an increase in the catalytic activity of these catalysts. For CPIL-TBP the yields were 71.4% for propylene carbonate and 78.7% for styrene carbonate. For CPIL-TBA the increase in propylene carbonate yield was 67.7%.<br>O uso das tecnologias de captura e armazenamento de carbono (CCS) e de captura utiliza??o do carbono (CCU) v?m sendo apontadas como possibilidades para mitigar os efeitos causados pela libera??o deliberada de elevadas concentra??es de CO2 na atmosfera. A utiliza??o do CO2 em rea??es de cicloadi??o em ep?xidos para obter carbonatos c?clicos vem se mostrando uma possibilidade interessante para reduzir a emiss?o de CO2 na atmosfera. Os carbonatos c?clicos s?o produtos de grande aplicabilidade como solventes na ind?stria qu?mica e s?o utilizados como mat?ria prima para uma vasta gama de produtos. Entretanto, o CO2 apresenta baixa reatividade tornando-se necess?rio o uso de catalisadores para otimizar tais rea??es. Os poli(l?quidos i?nicos) (PILs) v?m se mostrando como poss?veis catalisadores alternativos, demonstrando-se seletivos, recicl?veis e gerando consider?vel convers?o. Neste trabalho foram testados poli(l?quidos i?nicos) celul?sicos (CPIL), obtidos a partir da casca do arroz, como catalisador heterog?neo. A extra??o da celulose foi realizada por um m?todo qu?mico. A modifica??o qu?mica das fibras de celulose foi realizada a partir do tratamento com ?cido c?trico e a funcionaliza??o desta com 3 mmol dos l?quidos i?nicos BMIM Cl, TBAB, TBPB e BMPYRR formando CPIL-BMIM, CPIL-TBA, CPIL-TBP e CPIL-BMPYRR. As rea??es de cicloadi??o foram realizadas com os ?xidos de propileno e estireno com diferentes press?es de CO2 (15, 25, 30 e 40 bar) e temperaturas (90, 110, 120 e 130?C) durante (3, 6 e 9h), todas as rea??es foram realizadas sem a utiliza??o de solventes. O produto obtido foi caracterizado por cromatografia gasosa (CG) , espectroscopia de infravermelho com transformada de Fourier (FTIR) e Resson?ncia Magn?tica Nuclear (1H RMN). Os resultados demonstraram melhores rendimentos para CPIL-TBP e CPIL-TBA, cujos valores foram de 53,2% e 39% respectivamente para o carbonato de propileno e de 67,3% para o carbonato de estireno utilizando o CPIL-TBP. Quando o cocatalisador ZnBr2 foi utilizado, houve um aumento na a atividade catal?tica destes catalisadores, para o CPIL-TBP os rendimentos foram de 71,4 % para o carbonato de propileno e 78,7% para o carbonato de estireno. Para o CPIL-TBA o aumento no rendimento de carbonato de propileno foi de 67,7%.

The thesis presents an unconventional approach for the molecular construction using carbenes as the carbon source, and water as the hydrogen source. A gold-catalyst has been used to transfer the carbenes to effect [2,3]-sigmatropic rearrangement; and tris(pentafluorophenyl)borane-catalyst for the C-C bond functionalization and carbonate transfer reactions. On the other hand, a Pd-catalyzed stereodivergent reduction of alkynes has been achieved using water. The mechanistic details of the hydrogenation have also been probed, and the derived understanding has been applied to construct C-C bonds

Chapter 1: Metal carbenoids in organic synthesis The chapter describes the phenomena of metal carbenoid insertion reactions in two parts: Part A, and Part B. The study of N-tosylhydrazones as diazo precursor was commenced by Jose Barluenga in 2007,1 which demonstrated an in-situ generation of diazo species and trapping of that with low valent palladium catalyst (Scheme 1). Later, this palladium-carbenoid assumption was supported by few reports. Some of these discoveries were by D. F. Taber in 1986 followed by van Vranken in 1999 & 2001.2 These studies of palladium carbenes were supplemented by several groups in subsequent years. The consequent developments with N-tosylhydrazones as diazo source were very fruitful and produced exceptional chemical transformations in recent years. Though the precursor is also vastly customary for other metals such as Cu, Ni, Rh and Co, the primary focus has been given to Pd catalysis due to its wide utility and applicability. 1) Barluenga, J.; Moriel, P.; Valdes, C.; Aznar, F. Angew. Chem., Int. Ed. 2007, 46, 5587. 2) (a) Taber, D. F.; Amedio, J. C., Jr.; Sherrill, R. G. J. Org. Chem. 1986, 51, 3382. (b) Hoye, T. R.; Dinsmore, C. J.; Johnson, D. S.; Korkowski, P. F. J. Org. Chem. 1990, 55, 4518. (c) Greenman, K. L.; Carter, D. S.; Van Vranken, D. L Tetrahedron 2001, 57, 5219. 3) Palladium catalysed coupling of tosylhydrazones with aryl and heteroaryl halides in the absence of external ligands: synthesis of substituted olefins, Ojha, D. P.; Prabhu, K. R. J. Org. Chem., 2013, 78, 12136. Modes of reactivity of a metal-carbene Scheme 1 Cascade carbene migratory insertion process Part A: Ligand-free coupling of tosylhydrazones with aryl & heteroaryl halides In this part, Palladium catalysed cross-coupling reaction of hydrazones with aryl halides in absence of an external ligand is reported. The versatility of this coupling reaction has been demonstrated by showcasing the selectivity of coupling reaction in presence of hydroxyl and amine functional groups. This method allows synthesizing a variety of heterocyclic compounds, which are otherwise difficult to access from traditional methods. Application of the present methodology is validated in tandem reaction of ketones to the corresponding substituted olefins in a single pot experiment. Few examples are illustrated below in Scheme 2.3 Scheme 2: Scope of aryl halide coupling with tosylhydrazones Part B: Pd-catalysed Synthesis of Highly Branched Dienes The regioselective formation of highly branched dienes is a challenging task. Design and exploration of alternative working models to achieve such a regioselectivity to accomplish highly branched dienes is considered to be a historical advancement of Heck reaction to construct branched dienes. On the basis of the utility of carbene transfer reactions, in the reaction of hydrazones with Pd(II) under oxidative conditions, we envisioned obtaining a Pd-bis-carbene complex with α-hydrogens, which can lead to branched dienes. Herein, we report a novel Pd catalyzed selective coupling reaction of hydrazones in presence of tert-BuOLi and benzoquinone oxidant to form corresponding branched dienes (Scheme 3).4 The utility of the Pd catalyst for cross-coupling reactions for synthesizing branched conjugated dienes are rare. The reaction is very versatile and compatible with a variety of functional groups and is useful in synthesizing heterocyclic molecules. We anticipate that this Pd-catalyzed cross-coupling reaction will open new avenues for synthesizing useful compounds. 4) Pd-catalyzed cross-coupling reactions of hydrazones: regioselective synthesis of highly branched dienes, Ojha, D. P.; Prabhu, K. R. J. Org. Chem., 2012, 77, 11027. 5) Furrow, M. E.; Myers, A. G. J. Am. Chem. Soc. 2004, 126, 5436. 6) Taber, D. F.; Guo, P.; Guo, N. J. Am. Chem. Soc. 2010, 132, 11179. Scheme 3: diene synthesis via bis-carbene insertion process Chapter 2: Tosylhydrazones: Role in modern day organic synthesis In recent days, hydrazone based reactions are focused on the donor-acceptor ability of the hydrazones or the in-situ generated diazo species (Scheme 4). This commenced with the Myers’s report in 2004,5 which simplifies the Barton vinyl halide preparation with a remarkable revision on synthesis of alkyl-silyl-hydrazones and its applications. Improved methods of using tosylhydrazones were demonstrated by Aggarwal in successive years. Cycloadditions were implemented by Douglass F. Taber. 6 This study was enriched in a quite fascinating way by several groups such as Jose Barluenga, with many reductive coupling reactions and 1, 3-dipolar reactions. Thomson, in a very interesting report shows the traceless petasis reaction with hydrazones and also worked in many other prospects such as three component reactions and the acid catalysed [3+3] sigmatropic reactions of hydrazones. 7 Wang has also impressed with very attractive transformations in the past decade. 8 7) Thomson, R. J. et al. Nat. Chem. 2009, 1, 494. 8) Xiao, Q.; Zhang, Y.; Wang, J. Acc. Chem. Res. 2012, 46, 236. 9) Regioselective Synthesis of vinyl halides, vinyl sulfones, and alkynes: A tandem intermolecular nucleophilic and electrophilic vinylation of tosylhydrazones, Ojha, D. P.; Prabhu, K. R. Org. Lett. 2015, 17, 18. Scheme 4: Trapping diazo species in intermolecular fashion Part A: Synthesis of vinyl halides Trapping diazo species in an intermolecular fashion by attack of two independent ions (a cation followed by an anion) in tandem at the carbene center is unprecedented. As part of our efforts on the utility of tosylhydrazones, herein we report a novel approach of using ambiphilic diazo species to perform a tandem attack of a nucleophile followed by an electrophile in an intermolecular fashion for synthesizing various types of vinyl halides. A few representative examples are shown in Scheme 5.9 Scheme5: Synthesis if vinyl halides Part B: Synthesis of vinyl sulfones Vinyl sulfones are potential synthetic targets due to their presence in biologically and pharmaceutically important molecules ranging from small natural metabolites to proteins, and have found widespread applications in biological research as covalent protease inhibitors. Vinyl sulfones represent one of the important sulfur containing functional groups in organic chemistry, which are generally synthesized through elimination reactions, oxidation of vinyl sulfides or witting reactions using multistep sequence. Following this technique, we were able to synthesize a variety of vinyl sulfones with rich mechanistic features in a single step. A few such examples are documented in Scheme 6.9 Scheme 6: synthesis of vinyl sulfones Part C: Synthesis of alkynes The functional group conversion to achieve alkyne frameworks are generally a difficult transformation. There are very few limited and tedious processes are available in literature, mainly containing multi-step procedures. Additionally these reactions are require harsh conditions. Considering all these factors, there is a need for developing methods to synthesize alkynes from common functional groups under mild reactions conditions. In a similar way, to introduce different halogens at the same carbon, we expected the eliminations of the leaving groups in tandem formed alkynes. After extensive screening studies, it was pleasing to find that the reaction of tosylhydrazones with NCS−BTEAC, NBS−TBAB, or NIS−TBAI combination in presence of K2CO3 in dioxane as solvent at 110 °C can furnish corresponding acetylene derivatives in good yields. Few examples are shown in Scheme 7.9 Scheme 7: Trapping diazo species in intermolecular fashion Chapter 3: Pd catalysed hydroboration This chapter shows a hydroboration study of terminal alkynes in a highly regioselective manner (Scheme 8). Organoboron derivatives have become essential intermediates in organic and medicinal chemistry. Pioneering contributions are made by Brown and Akira Suzuki, who both instigated the development of new synthetic tools for the introduction of boron atoms onto organic molecules. 10 10) (a) Barbeyron, R.; Benedetti, E.; Cossy, J.; Vasseur, J.-J.; Arseniyadis, S.; Smietana, M. Tetrahedron 2014, 70, 8431. (b) Miyaura, N.; Suzuki, A. Chem. Rev. 1995, 95, 2457. 11) Pd-Catalysed regioselective borylation of alkynes: A ligand controlled synthesis of α- and β vinyl boronates (manuscript submitted). Scheme 8: possibility of site selectivity in hydroboration Part A: Pd-catalysed regioselective borylation of alkynes: A ligand controlled synthesis of α and β – vinyl boronates The metal catalyzed borylations of alkynes proceeds in a two-step process. Initially M-Bpin species undergo an addition onto the alkynes to generate organometallic species followed by quenching of the organometallic species with electrophiles. The addition M-Bpin species is regioselective governed by the steric and electronics factors of both metal complex as well as alkyne substituents. In this direction, a palladium catalysed α-selective borylation was achieved for terminal alkynes. A broad range of substrates were successfully borylated under optimized reaction conditions with very high selectivity. Interestingly, the selectivity was reversed to terminal site by using a NHC ligand. A few examples are shown in Scheme 9.11 Scheme 9: α & β-vinyl boronates Chapter 4: Pd/borane unit: Behavior towards isomerization vs reduction of alkenes This study presents a unique behaviour of palladium-boronate unit responsible for olefin chain walking and olefin reduction reactions (Scheme 10). The catalytic system stands efficient against both functionalized and unfunctionalized olefin isomerization as well as reductions. This study has been presented in two parts. Scheme 10: isomerization vs reduction Part A: Pd/ boronates or borane unit as efficient catalytic systems for olefin chain walk This study presents the behaviour of palladium-boronate unit responsible for olefin chain walking. The catalytic system is efficient for both functionalized and unfunctionalized olefin isomerizations (Scheme 11). Cycloisomerization of transient conjugated alkenes to synthesize heterocycles are prominent applications of this technique. The system describes a concept of olefin activation by coordination with Pd-borane complex, this complex assists in a facile [1,3]-hydride shift. This technique allows us to facilitate an isomerization in functionalized as well as unfunctionalized olefinic systems. Considering the substrates scope, the catalytic cycle tolerates various sensitive functional groups and shows good selectivity. In the following Scheme 11 few examples are depicted.12 12) Palladium/boron catalytic unit for olefin chain-walk (manuscript under preparation). Scheme 11: chain-walking of olefins. Part B: Palladium catalysed boronate promoted alkene reduction in water In this work, water has been employed as a source of hydrogen. The reduction of alkenes was achieved using Pd catalyst in presence of bis(pinacolato)diboron and H2O. In this aspect, the utility of water as hydrogen equivalent is the pertinent as well as beneficial with many advantages. Few representative examples are shown in Scheme 12.13 13) Pd-Catalysed homogeneous hydrogenation of olefins by using water as hydrogen source (manuscript under preparation). Scheme 12: synthesis of alkenes reduced products.

Chapter 1: Metal carbenoids in organic synthesis The chapter describes the phenomena of metal carbenoid insertion reactions in two parts: Part A, and Part B. The study of N-tosylhydrazones as diazo precursor was commenced by Jose Barluenga in 2007,1 which demonstrated an in-situ generation of diazo species and trapping of that with low valent palladium catalyst (Scheme 1). Later, this palladium-carbenoid assumption was supported by few reports. Some of these discoveries were by D. F. Taber in 1986 followed by van Vranken in 1999 & 2001.2 These studies of palladium carbenes were supplemented by several groups in subsequent years. The consequent developments with N-tosylhydrazones as diazo source were very fruitful and produced exceptional chemical transformations in recent years. Though the precursor is also vastly customary for other metals such as Cu, Ni, Rh and Co, the primary focus has been given to Pd catalysis due to its wide utility and applicability. 1) Barluenga, J.; Moriel, P.; Valdes, C.; Aznar, F. Angew. Chem., Int. Ed. 2007, 46, 5587. 2) (a) Taber, D. F.; Amedio, J. C., Jr.; Sherrill, R. G. J. Org. Chem. 1986, 51, 3382. (b) Hoye, T. R.; Dinsmore, C. J.; Johnson, D. S.; Korkowski, P. F. J. Org. Chem. 1990, 55, 4518. (c) Greenman, K. L.; Carter, D. S.; Van Vranken, D. L Tetrahedron 2001, 57, 5219. 3) Palladium catalysed coupling of tosylhydrazones with aryl and heteroaryl halides in the absence of external ligands: synthesis of substituted olefins, Ojha, D. P.; Prabhu, K. R. J. Org. Chem., 2013, 78, 12136. Modes of reactivity of a metal-carbene Scheme 1 Cascade carbene migratory insertion process Part A: Ligand-free coupling of tosylhydrazones with aryl & heteroaryl halides In this part, Palladium catalysed cross-coupling reaction of hydrazones with aryl halides in absence of an external ligand is reported. The versatility of this coupling reaction has been demonstrated by showcasing the selectivity of coupling reaction in presence of hydroxyl and amine functional groups. This method allows synthesizing a variety of heterocyclic compounds, which are otherwise difficult to access from traditional methods. Application of the present methodology is validated in tandem reaction of ketones to the corresponding substituted olefins in a single pot experiment. Few examples are illustrated below in Scheme 2.3 Scheme 2: Scope of aryl halide coupling with tosylhydrazones Part B: Pd-catalysed Synthesis of Highly Branched Dienes The regioselective formation of highly branched dienes is a challenging task. Design and exploration of alternative working models to achieve such a regioselectivity to accomplish highly branched dienes is considered to be a historical advancement of Heck reaction to construct branched dienes. On the basis of the utility of carbene transfer reactions, in the reaction of hydrazones with Pd(II) under oxidative conditions, we envisioned obtaining a Pd-bis-carbene complex with α-hydrogens, which can lead to branched dienes. Herein, we report a novel Pd catalyzed selective coupling reaction of hydrazones in presence of tert-BuOLi and benzoquinone oxidant to form corresponding branched dienes (Scheme 3).4 The utility of the Pd catalyst for cross-coupling reactions for synthesizing branched conjugated dienes are rare. The reaction is very versatile and compatible with a variety of functional groups and is useful in synthesizing heterocyclic molecules. We anticipate that this Pd-catalyzed cross-coupling reaction will open new avenues for synthesizing useful compounds. 4) Pd-catalyzed cross-coupling reactions of hydrazones: regioselective synthesis of highly branched dienes, Ojha, D. P.; Prabhu, K. R. J. Org. Chem., 2012, 77, 11027. 5) Furrow, M. E.; Myers, A. G. J. Am. Chem. Soc. 2004, 126, 5436. 6) Taber, D. F.; Guo, P.; Guo, N. J. Am. Chem. Soc. 2010, 132, 11179. Scheme 3: diene synthesis via bis-carbene insertion process Chapter 2: Tosylhydrazones: Role in modern day organic synthesis In recent days, hydrazone based reactions are focused on the donor-acceptor ability of the hydrazones or the in-situ generated diazo species (Scheme 4). This commenced with the Myers’s report in 2004,5 which simplifies the Barton vinyl halide preparation with a remarkable revision on synthesis of alkyl-silyl-hydrazones and its applications. Improved methods of using tosylhydrazones were demonstrated by Aggarwal in successive years. Cycloadditions were implemented by Douglass F. Taber. 6 This study was enriched in a quite fascinating way by several groups such as Jose Barluenga, with many reductive coupling reactions and 1, 3-dipolar reactions. Thomson, in a very interesting report shows the traceless petasis reaction with hydrazones and also worked in many other prospects such as three component reactions and the acid catalysed [3+3] sigmatropic reactions of hydrazones. 7 Wang has also impressed with very attractive transformations in the past decade. 8 7) Thomson, R. J. et al. Nat. Chem. 2009, 1, 494. 8) Xiao, Q.; Zhang, Y.; Wang, J. Acc. Chem. Res. 2012, 46, 236. 9) Regioselective Synthesis of vinyl halides, vinyl sulfones, and alkynes: A tandem intermolecular nucleophilic and electrophilic vinylation of tosylhydrazones, Ojha, D. P.; Prabhu, K. R. Org. Lett. 2015, 17, 18. Scheme 4: Trapping diazo species in intermolecular fashion Part A: Synthesis of vinyl halides Trapping diazo species in an intermolecular fashion by attack of two independent ions (a cation followed by an anion) in tandem at the carbene center is unprecedented. As part of our efforts on the utility of tosylhydrazones, herein we report a novel approach of using ambiphilic diazo species to perform a tandem attack of a nucleophile followed by an electrophile in an intermolecular fashion for synthesizing various types of vinyl halides. A few representative examples are shown in Scheme 5.9 Scheme5: Synthesis if vinyl halides Part B: Synthesis of vinyl sulfones Vinyl sulfones are potential synthetic targets due to their presence in biologically and pharmaceutically important molecules ranging from small natural metabolites to proteins, and have found widespread applications in biological research as covalent protease inhibitors. Vinyl sulfones represent one of the important sulfur containing functional groups in organic chemistry, which are generally synthesized through elimination reactions, oxidation of vinyl sulfides or witting reactions using multistep sequence. Following this technique, we were able to synthesize a variety of vinyl sulfones with rich mechanistic features in a single step. A few such examples are documented in Scheme 6.9 Scheme 6: synthesis of vinyl sulfones Part C: Synthesis of alkynes The functional group conversion to achieve alkyne frameworks are generally a difficult transformation. There are very few limited and tedious processes are available in literature, mainly containing multi-step procedures. Additionally these reactions are require harsh conditions. Considering all these factors, there is a need for developing methods to synthesize alkynes from common functional groups under mild reactions conditions. In a similar way, to introduce different halogens at the same carbon, we expected the eliminations of the leaving groups in tandem formed alkynes. After extensive screening studies, it was pleasing to find that the reaction of tosylhydrazones with NCS−BTEAC, NBS−TBAB, or NIS−TBAI combination in presence of K2CO3 in dioxane as solvent at 110 °C can furnish corresponding acetylene derivatives in good yields. Few examples are shown in Scheme 7.9 Scheme 7: Trapping diazo species in intermolecular fashion Chapter 3: Pd catalysed hydroboration This chapter shows a hydroboration study of terminal alkynes in a highly regioselective manner (Scheme 8). Organoboron derivatives have become essential intermediates in organic and medicinal chemistry. Pioneering contributions are made by Brown and Akira Suzuki, who both instigated the development of new synthetic tools for the introduction of boron atoms onto organic molecules. 10 10) (a) Barbeyron, R.; Benedetti, E.; Cossy, J.; Vasseur, J.-J.; Arseniyadis, S.; Smietana, M. Tetrahedron 2014, 70, 8431. (b) Miyaura, N.; Suzuki, A. Chem. Rev. 1995, 95, 2457. 11) Pd-Catalysed regioselective borylation of alkynes: A ligand controlled synthesis of α- and β vinyl boronates (manuscript submitted). Scheme 8: possibility of site selectivity in hydroboration Part A: Pd-catalysed regioselective borylation of alkynes: A ligand controlled synthesis of α and β – vinyl boronates The metal catalyzed borylations of alkynes proceeds in a two-step process. Initially M-Bpin species undergo an addition onto the alkynes to generate organometallic species followed by quenching of the organometallic species with electrophiles. The addition M-Bpin species is regioselective governed by the steric and electronics factors of both metal complex as well as alkyne substituents. In this direction, a palladium catalysed α-selective borylation was achieved for terminal alkynes. A broad range of substrates were successfully borylated under optimized reaction conditions with very high selectivity. Interestingly, the selectivity was reversed to terminal site by using a NHC ligand. A few examples are shown in Scheme 9.11 Scheme 9: α & β-vinyl boronates Chapter 4: Pd/borane unit: Behavior towards isomerization vs reduction of alkenes This study presents a unique behaviour of palladium-boronate unit responsible for olefin chain walking and olefin reduction reactions (Scheme 10). The catalytic system stands efficient against both functionalized and unfunctionalized olefin isomerization as well as reductions. This study has been presented in two parts. Scheme 10: isomerization vs reduction Part A: Pd/ boronates or borane unit as efficient catalytic systems for olefin chain walk This study presents the behaviour of palladium-boronate unit responsible for olefin chain walking. The catalytic system is efficient for both functionalized and unfunctionalized olefin isomerizations (Scheme 11). Cycloisomerization of transient conjugated alkenes to synthesize heterocycles are prominent applications of this technique. The system describes a concept of olefin activation by coordination with Pd-borane complex, this complex assists in a facile [1,3]-hydride shift. This technique allows us to facilitate an isomerization in functionalized as well as unfunctionalized olefinic systems. Considering the substrates scope, the catalytic cycle tolerates various sensitive functional groups and shows good selectivity. In the following Scheme 11 few examples are depicted.12 12) Palladium/boron catalytic unit for olefin chain-walk (manuscript under preparation). Scheme 11: chain-walking of olefins. Part B: Palladium catalysed boronate promoted alkene reduction in water In this work, water has been employed as a source of hydrogen. The reduction of alkenes was achieved using Pd catalyst in presence of bis(pinacolato)diboron and H2O. In this aspect, the utility of water as hydrogen equivalent is the pertinent as well as beneficial with many advantages. Few representative examples are shown in Scheme 12.13 13) Pd-Catalysed homogeneous hydrogenation of olefins by using water as hydrogen source (manuscript under preparation). Scheme 12: synthesis of alkenes reduced products.

The thesis entitled “Copper-Catalyzed Novel Oxidative Transformations: Construction of Carbon-Hetero Bonds” is divided into two main sections. Section A deals with the utility of azide as a nitrogen source for C-N bond formation, which is further divided into 4 chapters, and section B presents decarboxylative radical coupling reaction for C-heteroatom bond formation which is further divided in to two chapters. Section A Chapter 1 describes an approach for the direct synthesis of nitrile from the corresponding alcohols using azide as a nitrogen source. Nitrile functionality is a versatile and ubiquitous which occurs in a variety of natural products. Nitrile functionality can be easily transformed into a variety of functional groups and products such as aldehydes, ketones, acids, amines, amides and nitrogen-containing heterocycles, such as tetrazoles and oxazoles. In this chapter a successful attempt for developing a novel methodology to oxidize benzylic and cinnamyl alcohols to their corresponding nitriles in excellent yields has been described. This strategy uses DDQ as an oxidant and TMSN3 as a source of nitrogen in the presence of a catalytic amount of Cu(ClO4)2·6H2O. A few representative examples are highlighted in Scheme 1.1 Scheme 1. Oxidative conversion of alcohols to nitriles Second chapter represents a protocol for the synthesis of 1,5-disubstituted tetrazoles from the corresponding secondary alcohols. Among heterocyles, tetrazole and its derivatives are important class of nitrogen containing molecules. Due to their well-known biological activities as well as vast applications in pharmaceuticals and material science, they are potential targets for synthetic organic chemists. Therefore, a simple and user-friendly method for the synthesis of tetrazole is desirable. In this chapter, a mild and convenient method to synthesize 1,5-disubstituted tetrazoles using easily accessible secondary alcohols by employing TMSN3 as a nitrogen source is developed. This reaction is performed in the presence of a catalytic amount of Cu(ClO4)2·6H2O using DDQ as an oxidant under ambient conditions (Scheme 2).2 Scheme 2. Oxidative conversion of secondary alcohols to tetrazoles Third chapter presents a method for synthesizing amides from their corresponding secondary alcohols. Amide functionality is a crucial backbone in peptide chemistry, it also serve as an important precursor or intermediate for variety of organic transformations. In this contention, a mild and convenient method to synthesize amides using easily accessible secondary alcohols by employing TMSN3 as a nitrogen source is developed. This reaction is performed in the presence of a catalytic amount of Cu(ClO4)2·6H2O using DDQ as an oxidant under ambient conditions (Scheme 3).3 Scheme 3. Oxidative conversion of secondary alcohols to amides Additionally, the application of this methodology has also been revealed for the synthesis azides directly from their alcohols. Some of the representative examples are shown in the Scheme 4.3 Scheme 4. Direct conversion of alcohols to their azides. Fourth chapter describes highly chemoselective Schmidt reaction. The classical Schmidt reaction involves the formation of new carbon-nitrogen bonds in a reaction of a carbon-centred electrophile with hydrazoic acid followed by loss of nitrogen, which usually occurs via a rearrangement. It is well known that under the Schmidt reaction conditions, ketones and carboxylic acids are converted into their corresponding amides and amines respectively, whereas aldehydes furnish a mixture of formanilides and nitriles. In this chapter, Schmidt reaction of aldehydes to obtain their nitriles without formation of the corresponding formanilide is presented (Scheme 5).4 It was also observed that aromatic ketones and acids functionalities were intact under the reaction condition, unlike the conventional Schmidt reaction. Scheme 5. Highly chemoselective Schmidt reaction Section B It is divided into two chapters, describes a copper catalyzed decarboxylative radical coupling for the synthesis of vinyl sulfones and nitroolefins (Scheme 6). Scheme 6. General strategy for the second part First chapter narrates a strategy for synthesizing nitroolefins from the α,β-unsaturated carboxylic acids. Nitroolefins represent a unique class of nitro compounds, which have multifaceted utility in organic synthesis. They possess antibacterial, rodent-repelling, and antitumor activities. They serve as important intermediates in organic synthesis. Nitroolefins also react with a variety of nucleophiles, and their electron-deficient character renders them as a powerful dienophiles in Diels-Alder reactions. In our attempt to use the decarboxylative strategy, this chapter describes a method for the nitrodecarboxylation of substituted cinnamic acid derivatives to their corresponding nitroolefins. This nitrodecarboxylation reaction is performed using catalytic amount of CuCl in the presence of air using TBN as a nitrating source (Scheme 7).5 Besides, the reaction provides a useful method for the synthesis of β,β-disubstituted nitroolefin derivatives which are generally difficult to access from other conventional methods. Scheme 7. Decarboxylative nitration Second chapter presents a new protocol for the synthesis of vinyl sulfones from the α,β-unsaturated carboxylic acid. Vinyl sulfones are versatile building blocks, which find their utility as Michael acceptors and used in cycloaddition reactions. This functional group has also been shown to potently inhibit a variety of enzymatic processes, and thus provides unique properties for drug design and medicinal chemistry. Vinyl sulfones are prominent in medicinal chemistry owing to their wide presence in pharmaceutically active molecules, such as enzyme inhibitors and biological activity. In this chapter, we report a method for the construction of C-S bonds via ligand promoted decarboxylative radical sulfonylation of ,-unsaturated carboxylic acids to synthesize vinyl sulfones using Cu catalysis (Scheme 8).6 This is the first report for this particular conversion. Scheme 8. Decarboxylative sulfonation

The thesis entitled “Copper-Catalyzed Novel Oxidative Transformations: Construction of Carbon-Hetero Bonds” is divided into two main sections. Section A deals with the utility of azide as a nitrogen source for C-N bond formation, which is further divided into 4 chapters, and section B presents decarboxylative radical coupling reaction for C-heteroatom bond formation which is further divided in to two chapters. Section A Chapter 1 describes an approach for the direct synthesis of nitrile from the corresponding alcohols using azide as a nitrogen source. Nitrile functionality is a versatile and ubiquitous which occurs in a variety of natural products. Nitrile functionality can be easily transformed into a variety of functional groups and products such as aldehydes, ketones, acids, amines, amides and nitrogen-containing heterocycles, such as tetrazoles and oxazoles. In this chapter a successful attempt for developing a novel methodology to oxidize benzylic and cinnamyl alcohols to their corresponding nitriles in excellent yields has been described. This strategy uses DDQ as an oxidant and TMSN3 as a source of nitrogen in the presence of a catalytic amount of Cu(ClO4)2·6H2O. A few representative examples are highlighted in Scheme 1.1 Scheme 1. Oxidative conversion of alcohols to nitriles Second chapter represents a protocol for the synthesis of 1,5-disubstituted tetrazoles from the corresponding secondary alcohols. Among heterocyles, tetrazole and its derivatives are important class of nitrogen containing molecules. Due to their well-known biological activities as well as vast applications in pharmaceuticals and material science, they are potential targets for synthetic organic chemists. Therefore, a simple and user-friendly method for the synthesis of tetrazole is desirable. In this chapter, a mild and convenient method to synthesize 1,5-disubstituted tetrazoles using easily accessible secondary alcohols by employing TMSN3 as a nitrogen source is developed. This reaction is performed in the presence of a catalytic amount of Cu(ClO4)2·6H2O using DDQ as an oxidant under ambient conditions (Scheme 2).2 Scheme 2. Oxidative conversion of secondary alcohols to tetrazoles Third chapter presents a method for synthesizing amides from their corresponding secondary alcohols. Amide functionality is a crucial backbone in peptide chemistry, it also serve as an important precursor or intermediate for variety of organic transformations. In this contention, a mild and convenient method to synthesize amides using easily accessible secondary alcohols by employing TMSN3 as a nitrogen source is developed. This reaction is performed in the presence of a catalytic amount of Cu(ClO4)2·6H2O using DDQ as an oxidant under ambient conditions (Scheme 3).3 Scheme 3. Oxidative conversion of secondary alcohols to amides Additionally, the application of this methodology has also been revealed for the synthesis azides directly from their alcohols. Some of the representative examples are shown in the Scheme 4.3 Scheme 4. Direct conversion of alcohols to their azides. Fourth chapter describes highly chemoselective Schmidt reaction. The classical Schmidt reaction involves the formation of new carbon-nitrogen bonds in a reaction of a carbon-centred electrophile with hydrazoic acid followed by loss of nitrogen, which usually occurs via a rearrangement. It is well known that under the Schmidt reaction conditions, ketones and carboxylic acids are converted into their corresponding amides and amines respectively, whereas aldehydes furnish a mixture of formanilides and nitriles. In this chapter, Schmidt reaction of aldehydes to obtain their nitriles without formation of the corresponding formanilide is presented (Scheme 5).4 It was also observed that aromatic ketones and acids functionalities were intact under the reaction condition, unlike the conventional Schmidt reaction. Scheme 5. Highly chemoselective Schmidt reaction Section B It is divided into two chapters, describes a copper catalyzed decarboxylative radical coupling for the synthesis of vinyl sulfones and nitroolefins (Scheme 6). Scheme 6. General strategy for the second part First chapter narrates a strategy for synthesizing nitroolefins from the α,β-unsaturated carboxylic acids. Nitroolefins represent a unique class of nitro compounds, which have multifaceted utility in organic synthesis. They possess antibacterial, rodent-repelling, and antitumor activities. They serve as important intermediates in organic synthesis. Nitroolefins also react with a variety of nucleophiles, and their electron-deficient character renders them as a powerful dienophiles in Diels-Alder reactions. In our attempt to use the decarboxylative strategy, this chapter describes a method for the nitrodecarboxylation of substituted cinnamic acid derivatives to their corresponding nitroolefins. This nitrodecarboxylation reaction is performed using catalytic amount of CuCl in the presence of air using TBN as a nitrating source (Scheme 7).5 Besides, the reaction provides a useful method for the synthesis of β,β-disubstituted nitroolefin derivatives which are generally difficult to access from other conventional methods. Scheme 7. Decarboxylative nitration Second chapter presents a new protocol for the synthesis of vinyl sulfones from the α,β-unsaturated carboxylic acid. Vinyl sulfones are versatile building blocks, which find their utility as Michael acceptors and used in cycloaddition reactions. This functional group has also been shown to potently inhibit a variety of enzymatic processes, and thus provides unique properties for drug design and medicinal chemistry. Vinyl sulfones are prominent in medicinal chemistry owing to their wide presence in pharmaceutically active molecules, such as enzyme inhibitors and biological activity. In this chapter, we report a method for the construction of C-S bonds via ligand promoted decarboxylative radical sulfonylation of ,-unsaturated carboxylic acids to synthesize vinyl sulfones using Cu catalysis (Scheme 8).6 This is the first report for this particular conversion. Scheme 8. Decarboxylative sulfonation

碩士<br>國立中央大學<br>光電科學與工程學系<br>102<br>In this study, ECR-CVD was used for the deposition of high doping silicon thin films and passivation layers, PE-CVD was used for the deposition of passivation layers. These thin films were deposited on single-crystalline silicon substrate to fabricate the homo-junction and hetero-junction c-Si solar cells. The electrical properties and solar cell performance of homo-junction and hetero-junction c-Si solar cells were investigated. ECR-CVD has advantages about high deposition rate, low working pressure, low ion bombardment and no electrode pollution. But high deposition rate makes the thin films more defects and loose structure, these feature are not good for passivation layers in HIT solar cells. Therefore, the better quality passivation layers were deposited by PE-CVD to improve the HIT solar cells. In the first part, we will modulate the experimental parameters of doping concentration of BSF, oxygen content of emitter, single-crystalline silicon substrate types, and the electrode in homo-junction solar cells to investigate the electrical properties and conversion efficiency. The emitter and back surface field layers of homo-junction solar cell were deposited by ECR-CVD. The characteristics of homo-junction solar cell with screen printing electrode on p-type planar substrate were shown as follow: VOC = 0.616 V, JSC = 37.397 mA/cm2, F.F. = 75 %, efficiency = 17.29 % in the area of 1 cm2. In second part, the passivation quality on different wafer and the variation of the passivation layer thickness were performed in hetero-junction solar cells. The passivation layer were deposited by ECR-CVD or PE-CVD, the emitter and back surface field layers were deposited by ECR-CVD. In addition, the characteristics of hetero-junction solar cell on p-type planar substrate with 3 nm passivation layer deposited by PE-CVD that were shown as follow: VOC = 0.644 V, JSC = 34.04 mA/cm2, F.F. = 68 %, efficiency = 14.8 % in the area of 1 cm2.

The inverse demand hetero Diels-Alder reaction has been shown to be catalysed by commercially available, air-sensitive lanthanide Lewis acid catalysts such as Yb(fod)₃ . To date there have been no reported examples of enantioselective Lewis acid catalysed reactions of ethyl vinyl ether with crotonaldehyde, the inverse demand hetero Diels-Alder reaction of interest. This Diels-Alder reaction yields dihydropyran products which are key in the synthesis of a number of natural product target molecules, such as carbohydrates. The lack of stereospecificity achieved with known chiral NMR shift reagents has been attributed to fluxional behaviour of the ligands on the large lanthanide metal centre. This lack of conformational rigidity was proposed to be controlled by the use of sterically demanding C₃ symmetric multidentate ligand systems. By carefully designing a suitable ligand system the binding of the crotonaldehyde moiety in the Lewis acid catalysed reaction was anticipated to occur with facial selectivity, resulting in enantioselective Diels-Alder products. It was determined that lanthanide complexes of fluorinated β-diketone ligands with sufficiently low pKa's effectively catalyse the desired inverse demand hetero Diels-Alder reaction. These functionalities were incorporated into a multidentate, achiral, C₃ symmetric ligand system which upon lanthanide complexation was shown to be an air-stable efficient Lewis acid catalyst. Two chiral C₃ symmetric ligand systems were also prepared but were shown to be unsuitable for lanthanide substitution due to insufficiently robust functionalities present in the ligand framework, such as sulfonyl esters and Molecular modelling studies of these ligand systems reveal that the incorporation of camphor into the ligand framework is ideal for the preparation of a facially selective aldehyde binding site. Thus, concluding work probed other possible ligand functionalities which would result in camphor containing, chiral, C₃ symmetric ligand systems that are stable as lanthanide Lewis acid complexes. This new class of ligand promises to be of interest as chiral C₃ symmetric ligand systems for substitution on lanthanide metal centres have yet to be reported. This work summarises several useful synthetic strategies for the preparation of chiral C₃ symmetric ligands in multigram quantities. The application of these higher order symmetry ligands in enantioselective catalysis remains an important area of research.<br>Graduate

Les glucides constituent la classe de molécules organiques la plus abondante et ceux-ci jouent des rôles cruciaux dans divers processus biologiques. De part leur importance médicinale, la préparation des désoxy-sucres, des C-glycosides et des C-disaccharides est devenue un sujet de pointe en synthèse organique. De façon générale, cette thèse décrit une nouvelle synthèse de novo des 4-désoxy hexopyrannoses en plus de la préparation de C-glycosides biologiquement actifs. De plus, une attention particulière a été portée à la préparation de novo de 4-désoxy-C-disaccharides. Dans un premier temps, le catalyseur de Cr(III) de Jacobsen et un complexe binaphtol/titane ont été utilisés pour réaliser des hétéro-Diels-Alder énantiosélectives. Les dihydropyrannes ainsi générés ont été transformés en 4-désoxy hexopyrannoses présents dans la nature. De cette façon, un dérivé de l’acide ézoaminuroïque, un précurseur de la désosamine et de la néosidomycine, a été préparé suivant cette approche de novo. De plus, à titre comparatif, la néosidomycine a également été fabriquée selon une approche chiron, à partir du méthyl alpha-D-mannopyrannoside. Finalement, une évaluation biologique préliminaire de la néosidomycine a été effectuée sur une la concanavaline-A (Chapitre 2). Dans un deuxième temps, une allylation stéréosélective sur un aldéhyde lié via des liens C-C à une unité mannoside a permis de générer un alcool homoallylique. Cette dernière fonctionnalité a été transformée en 4-désoxy hexopyrannose de configuration D ou L. De cette façon, la préparation de pseudo 4-désoxy-C-disaccharides, de 4-désoxy-C-disaccharides et de pseudo 4-désoxy aza-C-disaccharides a facilement été réalisée. Les rapports diastéréoisomériques de la réaction d’allylation ont été déterminés en plus de la configuration absolue des nouveaux centres stéréogéniques formés. La transformation des alcools homoallyliques en pyrannes poly hydroxylés ou en lactames poly hydroxylés a été réalisée, en plus de la déprotection de certains membres de cette famille pour une évaluation biologique préliminaire sur la concanavaline-A (Chapitre 3). Finalement, la synthèse de C-glycosides biologiquement actifs a été réalisée selon deux volets: i) préparation de 3-C-mannopyrannosyl coumarines et ii) synthèse de C-galactosides, inhibiteurs de la lectine PA-IL. Pour ce faire, le couplage de Heck a été utilisé à partir d’un ester alpha,bêta-insaturé, attaché à une unité glycosidique via des liens C-C, pour générer un dérivé glycosyl cinnamate de méthyle. Cependant, lorsque le 2-iodophénol est utilisé comme partenaire de Heck, la coumarine correspondante a été isolée. Les dérivés C-galactopyrannosyl cinnamates de méthyle représentent de bons inhibiteurs monovalents de la PA-IL avec un Kd aussi bas que 37 micro M (Chapitre 4).<br>Carbohydrates represent a large family of organic molecules that play key roles in various biological processes. Due to their medicinal importance, preparation of deoxy-sugars, C-glycosides and C-disaccharides have become an important topic in organic synthesis. Mostly, this thesis presents a new de novo synthesis of 4-deoxy hexopyranoses, along with the preparation of biologically relevant C-glycosides. Moreover, a special attention has been focussed on the de novo synthesis of 4-deoxy-C-disaccharides. Firstly, Jacobsen Cr(III) catalyst and a binaphthol/titanium complex have been used to catalyze enantioselective hetero-Diels-Alder reactions. The dihydropyran thus formed has been transformed into naturally occurring 4-deoxy hexopyranoses. Therefore, the ezoaminuroic acid core, a desosamine precursor and neosidomycin have been prepared following a de novo approach. Moreover, as a comparative study, neosidomycin has also been synthesized using a chiron approach from methyl alpha-D-mannopyranoside. Finally, a preliminary biological evaluation of neosidomycin has been applied on concanavalin-A (Chapter 2). Secondly, homoallylic alcohols have been generated from a stereoselective allylation on aldehyde linked via C-C bonds to a mannoside residue. Then, the homoallylic alcohols have been transformed into 4-deoxy hexopyranoses in various configurations (D or L). Thereby, the syntheses of pseudo 4-deoxy-C-disaccharides, 4-deoxy-C-disaccharides and pseudo 4-deoxy aza-C-disaccharides have been easily performed. Determinations of the diastereoisomeric ratio of the allylation reactions along with the absolute configuration of the newly formed chiral center have been easily achieved. Various members of this new family have been deprotected for a preliminary biological evaluation on concanavalin-A (Chapter 3). Finally, the syntheses of relevant C-glycosides have been realized regarding two aspects: i) 3-C-mannopyranosyl coumarin synthesis and ii) synthesis of C-galactosides as PA-IL inhibitors. Methyl glycosyl cinnamates have been isolated using a Heck coupling on alpha,beta-insaturated ester linked to glycosidic moieties via C-C bonds. However, when 2-iodophenol is used as a Heck partner, the corresponding coumarins have been isolated. C-Galactosyl methyl cinnamate derivatives represent good monovalent inhibitors with Kd as low as 37 micro M against PA-IL (Chapter 4).

博士<br>國立中正大學<br>化學研究所<br>91<br>1.Studies in Synthesis of C-1 Substituted Fulvenes via the Oxidation-Addition of Haloacetyl Halide to fulveneketene Acetal. In contrast to the [2+2] cycloaddition of fulvenes and ketenes, fulveneketene acetal, 2-chloropentadienylidene-1,3-dioxalane, reacts with a-halo acyl halides to give various C-1 substituted fulvene. Herein we describe the first example of oxidative-addition of a-chloroacetyl chloride to fulveneketene acetal, and formal synthesis of the carbocyclic analogs of Captopril. 2.Traceless Solid-Phase Synthesis of Cyclopenta[c]quinolines and Cyclopenta[c]chromenes via Hetero [6+3] Cycloadditions of Fulvene. A Facile Approach to the 11-Heterosteroids Framework. The hetero [6+3] cycloaddition of fulvenes to benzoquinones and indoanilines provides an efficient route to the synthesis cyclopenta[c]-4H-chromen-8-ol, benzo[d]cyclopenta[e]-3H-3-azin-8-ol and other 11-hetero steroids. The structure of the cyclopenta[c]chromene skeleton was confirmed by the X-ray structure analysis of the p-bromobenzoate of 109. The traceless solid-phase synthesis of the scaldfold, consist of 110 examples, was achieved by the reaction of benzoquinones or iodoanilines with aminofulvene resin 140 which was prepared from polystyrene amino resin 138 via 2-step reaction.

Tese de doutoramento em Ciências Farmacêuticas, na especialidade de Farmacognosia e Fitoquímica, apresentada à Faculdade de Farmácia da Universidade de Coimbra<br>Cymbopogon citratus (DC). Stapf (Poaceae), commonly known as lemongrass, is a tropical perennial shrub originated from the Southeast Asia. This plant is reported to possess antifungal, insecticidal, anti-diabetic, anti-septic, anti-mutagenic, anti-carcinogenic activities as well as anti-inflammatory. In fact, aqueous extracts of dried leaves are used all over the year in folk medicine for the treatment of peptic ulcers and inflammatory conditions. Recently, some phenolic compounds, such as luteolin and apigenin glycosides and condensed tannins, were identified and related to both antioxidant and anti-inflammatory properties. The purposes of this work were to i) validate an analytical method for quantification of phenolic compounds of C. citratus; ii) study the influence of harvest time and plant quality on the phenolic composition and antioxidant activity; iii) characterize the tannins; iv) validate the traditional uses of lemongrass infusion as anti-inflammatory in vivo; v) obtain a topical formulation to evaluate the phenolic compounds permeation and their anti-inflammatory activity; vi) trace the pharmacokinetic profile of the main phenolic compounds in rats. Three different extracts: infusion (CcI), 50% aqueous ethanol (CcM50%) and ethanol (CcM100%) extracts were prepared and a simple and efficient RP-HPLC-PDA method was successfully validated for simultaneous identification and quantification of phenolic acids and flavonoids. Infusions were also obtained from different harvest dates (April, June, July, August and September) and quality grades (High, Medium and Low). It was verified that the content on polyphenols and the antioxidant capacity of CcI is strongly related with the quality of the plant. The total phenols assay showed a substantial loss from August to September. It was possible to find out the best month to harvest the plant to get the most of each phenolic group: April and June for hydroxycinnamic acids; June and September for flavonoids; June, July and August for tannins. Regardless the group of phenolic compound addressed, its content was always inversely proportional to the degree of leaves ageing. For all tested oxidant species, the high-quality samples exhibited the best antioxidant results. CcI was fractionated by column chromatography and polyphenol-rich fractions, namely phenolic acids (CcPA), flavonoids (CcF) and tannins (CcT) were obtained. CcT was characterized by HPLC-PDA-ESI/MSn, revealing the presence of proanthocyanidin hetero-dimers, along with some common procyanidin dimers. These hetero-dimeric flavan structures have been described for the first time in lemongrass and consist of apigeniflavan or luteoliflavan units linked to a flavanone, either naringenin or eriodictyol, and may occur as aglycone or glycosylated forms. For the in vivo assays, CcI, CcF and CcT were tested. CcI administered before and after ethanol stimulus, significantly reduced the incidence and severity of gastric lesions and, consequently, the ulcer index, corroborating the traditional medicinal use of this plant to ameliorate gastritis and/or peptic ulcers symptoms. On the other hand, CcI, CcF and CcT were orally administered to rats, in order to evaluate the anti-inflammatory effect at the carrageenan-induced paw edema assay. The observed effect by CcI (68.24 mg/kg), 82.30% of edema inhibition, was very similar to that obtained by the reference NSAID used (diclofenac, 10 mg/kg), 84.00%. On the other hand, flavonoid (7.42 mg/kg) and the tannin-rich (5.96 mg/kg) fractions significantly contributed for the anti-inflammatory activity on the edema volume (59 and 61%, respectively). The topical anti-inflammatory activity of CcI was also addressed. The results suggest that flavonoids, mainly, luteolin 7-O-neohesperidoside, cassiaoccidentalin B, carlinoside and cynaroside, may contribute to the topical anti-inflammatory effect. CcF (0.6%), CcT (0.3%) and CcF+CcT (0.66%+0.34%) topical formulations were also tested, and the results obtained suggest that tannins and flavonoids also possess a significant activity and that a synergistic mechanism of action may occur. In fact, edema inhibitions of 43%, 47% and 59% were respectively verified, being CcF+CcT effect very close to that of 1% diclofenac (65.9%). Pharmacokinetic analysis was performed in plasma, liver and kidney and showed that the compounds present in CcI are not detected in vivo after a single-dose oral administration. In contrary, the metabolites, luteolin 7-O-glucuronide and luteolin 3’-O-sulfate, present at the highest bioavailability, are probably the main responsible for the anti-inflammatory activity previously reported. In conclusion, this work has developed a method to quantify the phenolic compounds contained in C. citratus; pointed the importance of harvesting and storing the plant material, in order to take the maximum advantages from the phenolic compounds use; and demonstrated, in safe doses, its anti-inflammatory activity, using an in vivo approach, which supports the traditional use of lemongrass infusion. Furthermore, C. citratus leaves flavonoids and tannins were highlighted as bioactive compounds, encouraging the development of new anti-inflammatory drugs or nutraceuticals.