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1
Sharfi, Hadar, and Hagit Eldar-Finkelman. "Sequential phosphorylation of insulin receptor substrate-2 by glycogen synthase kinase-3 and c-Jun NH2-terminal kinase plays a role in hepatic insulin signaling." American Journal of Physiology-Endocrinology and Metabolism 294, no. 2 (February 2008): E307—E315. http://dx.doi.org/10.1152/ajpendo.00534.2007.
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Serine phosphorylation of insulin receptor substrate (IRS) proteins is a potential inhibitory mechanism in insulin signaling. Here we show that IRS-2 is phosphorylated by glycogen synthase kinase (GSK)-3. Phosphorylation by GSK-3 requires prior phosphorylation of its substrates, prompting us to identify the “priming kinase.” It was found that the stress activator anisomycin enhanced the ability of GSK-3 to phosphorylate IRS-2. Use of a selective c-Jun NH2-terminal kinase (JNK) inhibitor and cells overexpressing JNK implicated JNK as the priming kinase. This allowed us to narrow down the number of potential GSK-3 phosphorylation sites within IRS-2 to four regions that follow the motif SXXXSP. IRS-2 deletion mutants enabled us to localize the GSK-3 and JNK phosphorylation sites to serines 484 and 488, respectively. Mutation at serine 488 reduced JNK phosphorylation of IRS-2, and mutation of each site separately abolished GSK-3 phosphorylation of IRS-2. Treatment of H4IIE liver cells with anisomycin inhibited insulin-induced tyrosine phosphorylation of IRS-2; inhibition was reversed by pretreatment with the JNK and GSK-3 inhibitors. Moreover, overexpression of JNK and GSK-3 in H4IIE cells reduced insulin-induced tyrosine phosphorylation of IRS-2 and its association with the p85 regulatory subunit of phosphatidylinositol 3-kinase. Finally, both GSK-3 and JNK are abnormally upregulated in the diabetic livers of ob/obmice. Together, our data indicate that IRS-2 is sequentially phosphorylated by JNK and GSK-3 at serines 484/488 and provide evidence for their inhibitory role in hepatic insulin signaling.
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Baird, T. Regan, Margaret Jacobs, Adam Tinklepaugh, Peter Gross, Barbara C. Furie, and Bruce Furie. "A Novel Thrombin Fluorogenic Substrate of High Affinity, Catalytic Efficiency and Selectivity." Blood 106, no. 11 (November 16, 2005): 1953. http://dx.doi.org/10.1182/blood.v106.11.1953.1953.
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Abstract Thrombin is a serine protease with multiple functions, including the conversion of fibrinogen to fibrin, platelet activation, activation of Factor VIII and Factor V. Although many low molecular weight substrates have been developed for the study of thrombin catalytic activity, our interest in analyzing thrombin activity in the blood of a living mouse required development of a new class of thrombin substrates of high affinity and high selectivity whose product upon hydrolysis could be visualized by intravital fluorescence microscopy. We have developed a novel substrate for thrombin using the fluorochrome Alexa 488 and the quencher QSY35. Alexa 488 is conjugated to the N-terminus of a 12 amino acid peptide based upon the thrombin cleavage site in the α-chain of fibrinogen and the quencher is coupled to the C-terminus of the peptide, yielding Alexa 488-KGGVR-GPRVVEA-QSY35. We term this substrate FBG-12. Through fluorescence energy resonance transfer, the emission from Alexa 488 is absorbed by the quencher QSY 35, thus minimizing fluorescence, since the two moieties have overlapping spectral properties and are separated by a Förster radius less than 44 Å. Thrombin hydrolyzes the peptide yielding the N-terminal fragment, Alexa 488-KGGVR-COOH, which is highly fluorescent, and the C-terminal fragment NH2-GPRVVEA-QSY35 peptide which, being physically separated from the Alexa 488, no longer quenches the fluorochrome. The peptide was synthesized by solid phase peptide synthesis, its N-terminus and C-terminus were modified with Alexa 488 and QSY35 respectively, and its identity confirmed by mass spectroscopy and protein sequencing. The kinetic properties of FBG-12 were determined in vitro. Hydrolysis of the substrate by thrombin resulted in a linear increase in fluorescence at 525 nm over time and was dependent on enzyme concentration. The fluorescence of the product of thrombin hydrolysis of FBG-12 was 120-fold greater than that of the substrate. Michaelis-Menten kinetic analysis of thrombin hydrolysis of the fluorogenic substrate FBG-12 revealed a Km of 2 μM, a kcat of 759 s−1, and a kcat/Km of 3795 x 106 M−1 s−1. To determine the selectivity of this substrate, other plasma serine proteases were analyzed for their ability to hydrolyze FBG-12. Factor Xa, Factor VIIa, and activated protein C did not hydrolyze FBG-12. Factor XIa (Km 11 μM, kcat 20s−1, kcat/Km = 20 x 106 M−1 s−1) did hydrolyze FBG-12, although the reaction was inefficient compared to thrombin. Our characterization of FBG-12 suggests that this substrate is hydrolyzed efficiently and selectively by thrombin. Its spectral properties and solubility in a physiologic environment make FBG-12 a suitable substrate for the detection of thrombin activity via intravital imaging during thrombus formation in vivo.
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García, Javier, Sergio José Ibáñez, Sebastian Feu, María Cañadas, and Isabel Parejo. "Estudio de la gestoforma del lanzamiento a canasta en la liga E.B.A. (Study of shot technique in basketball in E.B.A. league)." Retos, no. 14 (March 28, 2015): 17–21. http://dx.doi.org/10.47197/retos.v0i14.35005.
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El objetivo fue analizar la relación existente entre la gestoforma de los lanzamientos en liga E.B.A. (liga española de baloncesto amateur) y una serie de variables recogidas durante la ejecución del mismo, i) periodo ii) cuarto, ii) eficacia, iv) presión defensiva, v) zona del campo, vi) rol del jugador que lanza, y vii) la acción previa que realiza. Se registraron un total de 8869 lanzamientos (167,40±21,45). Se encontraron relaciones estadísticamente significativas entre gestoforma y: i) eficacia del lanzamiento (c2(33, N=6598) = 365.287, p< .01;C= .229,p< .01), ii) presión defensiva (c2 (77, N=6598) = 365.287, p<.01; C = .229, p< .01), iii) gestoforma y zona del campo (c2(165, N=6598) = 2060.092,p< .01; C= .488, p< .01), iv) valor del lanzamiento (c2(11, N=6598) = 1148.784 p< .01, C= .385, p< .01), v) rol del jugador que lanza (c2(22, N=5394) = 226.979 p< .01;C= .201, p< .01), y vi) acción previa (c2(55, N=4331) = 1307.575 p< .01; C= .482, p< .01). Estos resultados contribuyen a mejorar el diseño de las sesiones de entrenamiento, ajustándolas a la situación real de juego.Abstract: The purpose of this study was to analyze shot technique in basketball in E.B.A. league (Spanish Basketball Amateur league), examining the relationship that exists between shot technique and a series of variables registered during this action, i) period, ii) quarter, iii) efficacy, iv) defensive pressure, v) zone of court, vi) position of player that took the shot, and vii) the previous action. A total of 8869 shots were registered (167,40±21,45). A significant relationship existed only between shot technique and: i) efficacy of the throw (c2(33, N=6598) = 365.287, p< .01;C= .229, p< .01), ii) defensive pressure (c2 (77, N=6598) = 365.287, p<.01; C = .229, p< .01), iii) zone of court (c2(165, N=6598) = 2060.092, p< .01; C= .488, p< .01), iv) value of the throw (c2(11, N=6598) = 1148.784 p< .01, C= .385, p< .01), v) position of player that took the shot (c2(22, N=5394) = 226.979 p< .01;C= .201, p< .01), and vi) the previous action (c2(55, N=4331) = 1307.575 p< .01; C= .482, p< .01). These results contribute to improve the design of the sessions of training, adjusting them to the real situation of game.
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Knight, K. S., C. N. W. Darlington, and I. G. Wood. "The crystal structure of KCaF3 at 4.2 and 300 K: A re-evaluation using high-resolution powder neutron diffraction." Powder Diffraction 20, no. 1 (March 2005): 7–13. http://dx.doi.org/10.1154/1.1835959.
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The crystal structure of the perovskite phase KCaF3 has been redetermined at 4.2 and 300 K using powder neutron diffraction collected at the highest resolution. At both temperatures the phase was found to be orthorhombic in space group Pnma, with lattice parameters a=0.622 879(5) nm, b=0.870 031(7) nm, c=0.611 210(5) nm at 4.2 K, and a=0.621 488(6) nm, b=0.876 360(8) nm, c=0.616 481(6) nm at 300 K. The CaF6 octahedron is regular at both temperatures with octahedral rotations of 9.6° and 13.2° for the in-phase and anti-phase tilts, respectively, at 4.2 K. No evidence was found to support the recent revision of the space group from Pnma to the monoclinic space group B21∕m.
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YANAGAWA, Shusuke, Ryota SHIMIZU, Mototsugu HAMADA, Toru SHIMIZU, and Tadahiro KURODA. "Optimization of Resonant Capacitance in Wireless Power Transfer System with 3-D Stacked Two Receivers." IEICE Transactions on Electronics E101.C, no. 7 (July 1, 2018): 488–92. http://dx.doi.org/10.1587/transele.e101.c.488.
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Duboise, S. Monroe, Jie Guo, Sue Czajak, Ronald C. Desrosiers, and Jae U. Jung. "STP and Tip Are Essential for Herpesvirus Saimiri Oncogenicity." Journal of Virology 72, no. 2 (February 1, 1998): 1308–13. http://dx.doi.org/10.1128/jvi.72.2.1308-1313.1998.
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ABSTRACT Mutant forms of herpesvirus saimiri (HVS) subgroup C strain 488 with deletions in either STP-C488 or Tip were constructed. The transforming potentials of the HVS mutants were tested in cell culture and in common marmosets. Parental HVS subgroup C strain 488 immortalized common marmoset T lymphocytes in vitro to interleukin-2-independent growth, but neither of the deletion mutants produced such growth transformation. Wild-type HVS produced fatal lymphoma within 19 to 20 days of experimental infection of common marmosets, while HVS ΔSTP-C488 and HVS ΔTip were nononcogenic. Virus was repeatedly isolated from the peripheral blood of marmosets infected with mutant virus for more than 5 months. These results demonstrate that STP-C488 and Tip are not required for replication or persistence, but each is essential for transformation in cell culture and for lymphoma induction in common marmosets.
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Miyoshi, Takashi, Shingo Masui, Takeshi Okada, Tomoya Yanamoto, Tokuya Kozaki, Shin-ichi Nagahama, and Takashi Mukai. "InGaN-based 518 and 488 nm laser diodes on c-plane GaN substrate." physica status solidi (a) 207, no. 6 (May 21, 2010): 1389–92. http://dx.doi.org/10.1002/pssa.200983446.
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Lal, Rattan. "E.R. Landa and C. Feller, Editors, Soil and Culture, Springer, Dordrecht (2010) ISBN 978-90-481-2959-1 488 p." Soil and Tillage Research 110, no. 2 (November 2010): 257–58. http://dx.doi.org/10.1016/j.still.2010.08.001.
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Latta, Steven C. "Birds of Aruba, Bonaire, and Curaçao: A Site and Field Guide." Journal of Caribbean Ornithology 30, no. 2 (May 13, 2018): 154–55. http://dx.doi.org/10.55431/jco.2017.30(2).154-155.
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BIRDS OF ARUBA, BONAIRE, AND CURAÇAO: A SITE AND FIELD GUIDE.—Jeffrey V. Wells and Allison Childs Wells. 2017. Cornell University Press, Ithaca, NY. 488 pp. ISBN: 978-1-5017-0107-8. $39.95.
Review by: Steven C. Latta
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Wang, Lixiu, Lu Zhao, Yan Zhang, Zhipeng Yao, Tao Li, Muhua Cao, Ruishuang Ma, et al. "Phosphatidylserine Exposing-Blood Cells and Microparticles Induce Procoagulant Activity in Non-Valvular Atrial Fibrillation." Blood 128, no. 22 (December 2, 2016): 715. http://dx.doi.org/10.1182/blood.v128.22.715.715.
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Abstract Introduction: Atrial fibrillation (AF) is the most common sustained arrhythmia in clinical practice, contributing directly to morbidity and mortality largely through thromboembolism events such as stroke. The risk of stroke in patients with AF is five times higher than in patients without AF. The pathophysiological of thromboembolism in AF is multifactorial and is not only related to stasis in a poorly contractile left atrium. Indeed, there is an increasing body of evidence to support that Virchow triad, including abnormal blood stasis, endothelial damage, and changes in blood constituents, is the main cause of thromboembolism or prothrombotic state in AF patients. However, relatively little is known about the precise role of phosphatidylserine (PS), one of the blood constituents, in the pro-thrombotic state or hypercoagulability of non-valvular AF (NVAF). Our objectives were to study the increased PS exposure on microparticles (MPs) and the outer membrane of MP-origin blood cells in NVAF patients, and to evaluate their procoagulant activity (PCA). Methods: Our study included NVAF patients without (n = 60) and with left atrial thrombosis (n = 30) and healthy controls (n = 30). PS exposure on MPs and blood cells was analyzed with flow cytometry and confocal microscopy. PCA was evaluated using clotting time, extrinsic/intrinsic FXa and prothrombinase production, and fibrin formation assays. Inhibition assays of PCA of PS+ MPs and blood cells were performed using lactadherin. Results: The number of PS+ MPs was significantly higher in NVAF patients (1.8-fold, P < 0.001) and NVAF patients with left atrial thrombosis (2.7-fold, P < 0.001) compared with healthy subjects, respectively. Furthermore, the number of PS+ MPs in NVAF patients with left atrial thrombosis was significantly higher than that in NVAF patients without (1.4-fold, P < 0.01, Figure 1A). Moreover, the PS+ MPs were originated from platelets (CD41a+, PMPs), neutrophils (CD66b+, NMPs), mononuclear cells (CD14+, MMPs), lymphocytes (CD3/19+, LMPs), erythrocytes (CD235a+, ErMPs), and endothelial cells (CD31+ CD41a-, EMPs) (Figure 1B). The levels of PS+ platelets, neutrophils, mononuclear cells, lymphocytes, and erythrocytes were significantly higher (all P < 0.001) in each NVAF group than those in healthy controls. And the levels of PS+ platelets, neutrophils, and erythrocytes in NVAF patients with left atrial thrombosis were significantly higher than those in NVAF patients (all P < 0.01, Figure 1C). In addition, circulating PS+ MPs cooperated with PS+ blood cells, contributing to markedly shortened coagulation time and dramatically increased FXa/thrombin generation and fibrin formation in each NVAF group (all P < 0.01). Moreover, blockade of exposed PS on MPs and blood cells with lactadherin inhibited PCA by approximately 80%. Conclusions: Our results suggest that increased PS exposure on MPs and blood cells play a procoagulant role in NVAF patients with or without left atrial thrombosis. Blockade of PS prior to left atrial thrombosis could become a novel therapeutic strategy to prevent thrombosis in these patients. Figure 1 Flow cytometry analyses of PS+ MPs and blood cells in each NVAF group and control subjects. (A and B) Events were selected for lactadherin-Alexa Fluor 488 binding. Lactadherin-positive MPs were further examined for expression of other antigens by co-labeling with Alexa Fluor 488- and Alexa Fluor 647-labeled antibodies as follows: PMPs (Alexa Fluro 647-CD41a+), NMPs (Alexa Fluro 647-CD66b+), MMPs (Alexa Fluro 488-CD14+), LMPs (Alexa Fluor 647-CD3+/Alexa Fluor 488-CD19+), ErMPs (Alexa Fluor 488-CD235a+), and EMPs (Alexa Fluro 488-CD31+/Alexa Fluor 647-CD41a-). (C) Lactadherin-binding counts of PLT/PMN/MNC/LYM/RBC from controls (n = 30), NVAF patients without (n = 60) and with left atrial thrombosis (n = 30) were measured. MPs, microparticles; NVAF, non-valvular atrial fibrillation; Thr, thrombosis; PMPs, platelet-derived MPs; NMPs, neutrophil-derived MPs; MMPs, mononuclear cell-derived MPs; LMPs, lymphocyte-derived MPs; ErMPs, erythrocyte-derived MPs; EMPs, endothelial-derived MPs; PLT, platelet; PMN, polymorphomuclear cells; MNC, mononuclear cell; LYM, lymphocyte; RBC, red blood cell. *P <0.001 vs. controls. #P< 0.01 vs. NVAF with Thr. Figure 1. Flow cytometry analyses of PS+ MPs and blood cells in each NVAF group and control subjects. (A and B) Events were selected for lactadherin-Alexa Fluor 488 binding. Lactadherin-positive MPs were further examined for expression of other antigens by co-labeling with Alexa Fluor 488- and Alexa Fluor 647-labeled antibodies as follows: PMPs (Alexa Fluro 647-CD41a+), NMPs (Alexa Fluro 647-CD66b+), MMPs (Alexa Fluro 488-CD14+), LMPs (Alexa Fluor 647-CD3+/Alexa Fluor 488-CD19+), ErMPs (Alexa Fluor 488-CD235a+), and EMPs (Alexa Fluro 488-CD31+/Alexa Fluor 647-CD41a-). (C) Lactadherin-binding counts of PLT/PMN/MNC/LYM/RBC from controls (n = 30), NVAF patients without (n = 60) and with left atrial thrombosis (n = 30) were measured. MPs, microparticles; NVAF, non-valvular atrial fibrillation; Thr, thrombosis; PMPs, platelet-derived MPs; NMPs, neutrophil-derived MPs; MMPs, mononuclear cell-derived MPs; LMPs, lymphocyte-derived MPs; ErMPs, erythrocyte-derived MPs; EMPs, endothelial-derived MPs; PLT, platelet; PMN, polymorphomuclear cells; MNC, mononuclear cell; LYM, lymphocyte; RBC, red blood cell. *P <0.001 vs. controls. #P< 0.01 vs. NVAF with Thr. Disclosures No relevant conflicts of interest to declare.
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Li, Wei, Shan You Li, and Zhen Zhao. "Investigation on Seismic Structural Damages in Anchang County in Wenchuan Earthquake." Advanced Materials Research 671-674 (March 2013): 1351–55. http://dx.doi.org/10.4028/www.scientific.net/amr.671-674.1351.
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The seismic damage investigation on 488 buildings in Anchang County during Wenchuan earthquake was performed and the investigation data were analyzed, especially for the school buildings. The results indicate that the seismic damages of buildings with seismic design are obviously lower than those of buildings without seismic design, and the damages of the Type-A buildings are most severe, those of the Type-B buildings are second while those of the Type-C buildings are the slightest. Moreover, the damages of Type-B and Type-C school buildings are a little higher than the average level of Type-B and C buildings in Anchang County.
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Mason, PJ, MF Sonati, D. MacDonald, C. Lanza, D. Busutil, M. Town, CM Corcoran, JS Kaeda, DJ Stevens, and S. al-Ismail. "New glucose-6-phosphate dehydrogenase mutations associated with chronic anemia." Blood 85, no. 5 (March 1, 1995): 1377–80. http://dx.doi.org/10.1182/blood.v85.5.1377.bloodjournal8551377.
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We have identified the glucose-6-phosphate dehydrogenase mutations responsible for enzyme deficiency in nine individuals with chronic nonspherocytic hemolytic anemia. We found the variants Tokyo, Iowa, Shinshu, and Guadalajara in British subjects and Kobe in an Italian. In addition we have determined the variant Corum has the mutation 820 G-->A and have found in British subjects the mis-sense mutations 224 T-->C, 488 G-->A and 833 C-->T which have not been described before. Some, but not all, of the mutations involve amino acids located near putative substrate binding sites.
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Kopyrulina, M. E., E. N. Zakharova, T. N. Zabotina, D. Yu Blokhin, and P. K. Ivanov. "Тhe technology of creation and quality testing of immunofluorescent probes with dye alexa-488 for analysis of celular populations by flow cytometry." Russian Journal of Biotherapy 17, no. 1 (November 27, 2018): 70–75. http://dx.doi.org/10.17650/1726-9784-2018-17-1-70-75.
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Fluorescent probes based on monoclonal antibodies (MAb) are widely used in scientific and clinical research in the field of oncology, hematology, immunology, epidemiology. Objective: to create of fluorescent probes based on the MAb and the fluorescent dye Alexa-488 for the analysis of cellular populations by flow cytometry. Materials and methods. MAb to B lymphocyte antigen (clone ICO-180), fluorescent dye Alexa-488 were used in the work. MAb was isolated from ascitic fluid by combined purification of the immunoglobulin fraction with caprylic acid and salting out with ammonium sulfate. Gel filtration on a PD-10 column was used to purify the conjugates (immunofluorescent probes, IFP), the concentration and labeling density of the IFP were determined spectrophotometrically. The determination of the working titer of the IFP was performed using the antibody titration method proposed by C.C. Stewart. Results. The optimal time of incubation of MAb with a fluorophore was experimentally determined. The optimal conditions for labeling MAb of the IСO series with the dye are: a carbonate buffer with pH 8,3, the concentration of antibodies in the reaction mixture is 1 mg/ml, molar ratio of active dye – 10–100 mmol per 1 mmol of protein, the incubation time is 90 minutes, the temperature is 18–25 °C. We obtained a panel of conjugates of MAb with Alexa-488, differing in their different labeling densities. Evaluation of the biological activity of the resulting conjugates was carried out on peripheral blood cells of donors in the concentration range of MAb 0,5–100 μg/ml. Conclusion. The optimal conditions for labeling MAb of the IСO series with the dye are: a carbonate buffer with pH 8,3, the concentration of antibodies in the reaction mixture is 1 mg/ml, the incubation time is 90 minutes, the temperature is 18–25 °C. The optimum density of labeling is in the range 5–13,5 M:M, the optimal concentration of antibodies is in the range of 5–25 μg/ml.
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Gazdová, Veronika, Petr Humpolíček, Vanda Déduchová, Jitka Filkuková, and Josef Dvořák. "Effect of C-CSN and B-CSN genotypes on milk production traits in Czech Flekvieh and Holstein breed." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 55, no. 1 (2007): 55–58. http://dx.doi.org/10.11118/actaun200755010055.
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The effect of the χ-casein (C-CSN) and β –casein (B-CSN) loci on the milk production traits (milk yield, fat, protein and lactose content) was estimated for 807 and 488 Czech Fleckvieh and 402 and 244 Holstein cows, respectively. Genotypes of C-CNS and B-CNS were determined by the use of PCR–RFLP method. The genotypes were detected by use of electrophoresis on agarose gel. The associations of studied polymorphisms with milk production traits were estimated using the mixed linear model procedure REML in SAS for Windows 9.1.3. Results indicated that protein content is significantly affected (P ≤ 0.01) by C-CSN genotype (genotype BB > AB > AA). Fat and lactose content were not affected by C-CSN locus. The B-CNS locus had no significant effect on any milk production traits.
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Dubert-Ferrandon, Alix, Keshaven Niranjan, and Alistair S. Grandison. "A novel technique for differentiation of proteins in the development of acid gel structure from control and heat treated milk using confocal scanning laser microscopy." Journal of Dairy Research 73, no. 4 (July 12, 2006): 423–30. http://dx.doi.org/10.1017/s0022029906001907.
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The incorporation of caseins and whey proteins into acid gels produced from unheated and heat treated skimmed milk was studied by confocal scanning laser microscopy (CSLM) using fluorescent labelled proteins. Bovine casein micelles were labelled using Alexa Fluor 594, while whey proteins were labelled using Alexa Fluor 488. Samples of the labelled protein solutions were introduced into aliquots of pasteurised skim milk, and skim milk heated to 90 °C for 2 min and 95 °C for 8 min. The milk was acidified at 40 °C to a final pH of 4·4 using 20 g glucono-delta-lactone/l (GDL). The formation of gels was observed with CSLM at two wavelengths (488 nm and 594 nm), and also by visual and rheological methods. In the control milk, as pH decreased distinct casein aggregates appeared, and as further pH reduction occurred, the whey proteins could be seen to coat the casein aggregates. With the heated milks, the gel structure was formed of continuous strands consisting of both casein and whey protein. The formation of the gel network was correlated with an increase in the elastic modulus for all three treatments, in relation to the severity of heat treatment. This model system allows the separate observation of the caseins and whey proteins, and the study of the interactions between the two protein fractions during the formation of the acid gel structure, on a real-time basis. The system could therefore be a valuable tool in the study of structure formation in yoghurt and other dairy protein systems.
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Sid Ahmed, Mazen A., Faisal Ahmad Khan, Hamad Abdel Hadi, Sini Skariah, Ali A. Sultan, Abdul Salam, Abdul Latif Al Khal, et al. "Association of blaVIM-2, blaPDC-35, blaOXA-10, blaOXA-488 and blaVEB-9 β-Lactamase Genes with Resistance to Ceftazidime–Avibactam and Ceftolozane–Tazobactam in Multidrug-Resistant Pseudomonas aeruginosa." Antibiotics 11, no. 2 (January 19, 2022): 130. http://dx.doi.org/10.3390/antibiotics11020130.
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Ceftazidime–avibactam and ceftolozane–tazobactam are approved for the treatment of complicated Gram-negative bacterial infections including multidrug-resistant (MDR) Pseudomonas aeruginosa. Resistance to both agents has been reported, but the underlying mechanisms have not been fully explored. This study aimed to correlate β-lactamases with phenotypic resistance to ceftazidime–avibactam and/or ceftolozane–tazobactam in MDR-P. aeruginosa from Qatar. A total of 525 MDR-P. aeruginosa isolates were collected from clinical specimens between 2014 and 2017. Identification and antimicrobial susceptibility were performed by the BD PhoenixTM system and gradient MIC test strips. Of the 75 sequenced MDR isolates, 35 (47%) were considered as having difficult-to-treat resistance, and 42 were resistant to ceftazidime–avibactam (37, 49.3%), and/or ceftolozane–tazobactam (40, 53.3%). They belonged to 12 sequence types, with ST235 being predominant (38%). Most isolates (97.6%) carried one or more β-lactamase genes, with blaOXA-488 (19%) and blaVEB-9 (45.2%) being predominant. A strong association was detected between class B β-lactamase genes and both ceftazidime–avibactam and ceftolozane–tazobactam resistance, while class A genes were associated with ceftolozane–tazobactam resistance. Co-resistance to ceftazidime–avibactam and ceftolozane–tazobactam correlated with the presence of blaVEB-9, blaPDC-35, blaVIM-2, blaOXA-10 and blaOXA-488. MDR-P. aeruginosa isolates resistant to both combination drugs were associated with class B β-lactamases (blaVIM-2) and class D β-lactamases (blaOXA-10), while ceftolozane–tazobactam resistance was associated with class A (blaVEB-9), class C (blaVPDC-35), and class D β-lactamases (blaOXA-488).
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Turner, D. G., G. I. Mandushev, and D. Forbes. "Erratum - Galactic Clusters with Associated Cepheid Variables - Part Four - C:2128+488 Anon Platais and V1726-CYGNI." Astronomical Journal 108 (December 1994): 2373. http://dx.doi.org/10.1086/117250.
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Spyratos, F., K. Hacène, V. Le Doussal, L. Durcos, M. Ferrero-Poüs, C. Bouchet, and J. Rouëssé. "PP-9-5 Expression of uPA, c-erbB2, EGF-R and p53 in 488 primary breast cancer." European Journal of Cancer 32 (January 1996): 56. http://dx.doi.org/10.1016/0959-8049(96)84274-2.
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DONDERS, JACOBUS, and MICHAEL T. MINNEMA. "Performance discrepancies on the California Verbal Learning Test–Children's Version (CVLT–C) in children with traumatic brain injury." Journal of the International Neuropsychological Society 10, no. 4 (July 2004): 482–88. http://dx.doi.org/10.1017/s1355617704104025.
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One hundred sixty-seven children with traumatic brain injury (TBI), selected from an 8-year series of consecutive referrals to a Midwestern rehabilitation hospital, completed the California Verbal Learning Test–Children's Version (CVLT–C) and the Wechsler Intelligence Scale for Children–Third Edition (WISC–III) within 1 year after injury. A large proactive interference (PI) effect, defined as performance on the second list that was at least 1.5 standard deviations below that on the 1st one, was statistically significantly more common in this clinical sample (21%) than in the CVLT–C standardization sample (11%). Other performance discrepancies, including retroactive interference, rapid forgetting, and retrieval problems, occurred at approximately the same rate in the clinical and standardization samples. Children with anterior cerebral lesions were about 3 times less likely to have a large PI effect than children without such lesions, but the former group performed worse on the first CVLT–C list. The impact of pediatric TBI on a wide range of CVLT–C quantitative variables was mediated by speed of information processing, as assessed by the WISC–III Processing Speed factor index. It is concluded that failure to release from PI is somewhat common, although certainly not universal, in children with TBI. Unlike with adults, anterior cerebral lesions are not associated selectively with an increased risk for PI after pediatric TBI but rather with a reduced efficiency of allocation of cognitive resources. Deficits in speed of information processing appear to be primarily responsible for the learning deficits on the CVLT–C after pediatric TBI. (JINS, 2004, 10, 482–488.)
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Takeyama, Masahiro, Evgueni L. Saenko, Katsumi Nishiya, Kenichi Ogiwara, Midori Shima, and Keiji Nogami. "Identification of a protein S-interactive site within the A2 domain of the factor VIII heavy chain." Thrombosis and Haemostasis 102, no. 10 (2009): 645–55. http://dx.doi.org/10.1160/th09-03-0152.
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SummaryWe have recently demonstrated that protein S impairs the intrinsic tenase complex, independent of activated protein C, in competitive interactions between the A2 and A3 domains of factor VIIIa and factor IXa. In the present study, we have identified a protein S-interactive site in the A2 domain of factor VIIIa. Anti-A2 monoclonal antibody recognising a factor IXa-functional region (residues 484–509) on A2, and synthetic peptide inhibited the A2 binding to protein S by ∼60% and ∼70%, respectively, in solid-phase binding assays. The 484–509 peptide directly bound to protein S dose-dependently. Covalent cross-linking was observed between the 484–509 peptide and protein S following reaction with EDC (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide). The cross-linked adduct was consistent with 1:1 stoichiometry of reactants. Cross-linking formation was blocked by addition of the 484–497 peptide, but not by the 498–509 peptide. Furthermore, N-terminal sequence analysis of the 484–509 peptide-protein S adduct showed that three sequential residues (S488, R489, and R490) in A2 were not identified, suggesting that these residues participate in cross-link formation. Mutant A2 molecules where these residues were converted to alanine were evaluated for the binding of protein S. The S488A, R489A, and R490A mutants demonstrated ∼four-fold lower affinity than wild-type A2.These results indicate that the 484–509 region in the A2 domain of factor VIIIa, in particular sequential residues at positions 488–490, contributes to a unique protein S-interactive site.
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Lovell, Gillian F., Nicola Bevan, Hinnah Campwala, Vincent Blancheteau, Tim Dale, Nicholas Dana, Nevine Holtz, Eric Endlsey, and D. J. Trezise. "Use of fluorescent Fab/Ab complexes and IncuCyte live-cell analysis to dynamically track cell surface markers and cell populations in mixed cultures." Journal of Immunology 200, no. 1_Supplement (May 1, 2018): 46.11. http://dx.doi.org/10.4049/jimmunol.200.supp.46.11.
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Abstract Fluorescently-labeled antibodies are widely used for visualising cellular protein expression/distribution (e.g. immunocytochemistry) and immuno-phenotyping (e.g. flow cytometry). However, their applications are largely confined to end-point or short term (min-h) detection, and the cell processing and labeling steps that are required often perturb the biology of interest. To enable longer-term, fully kinetic applications of cell surface protein markers in living cells, we have developed a novel strategy based on fluorescently-labeled antibody fragments (Fabs) and IncuCyte live-cell analysis. An Fc-targeted anti-mouse Fab fragment conjugated to a green-emitting fluoroprobe (IncuCyte FabFluor-488) was used to tag Abs to surface markers (e.g. CD4, CD20, Her2) via a simple one-step, no-wash protocol. Addition of the corresponding FabFluor-488-Ab complex to living cells (e.g. Jurkats, RAMOS, SKOV-3) produced long lasting (up to 5d), specific and stable cellular fluorescence at relatively low Ab concentrations (1mg ml−1) that did not perturb cell morphology or growth (IncuCyte S3). To illustrate the application of this approach the method was used to (a) quantify upregulation of the checkpoint protein PDL-1 over 48h in IFN-gamma-treated MDA-MB231 and SKOV-3 and tumor cells, (b) identify cellular subsets in PBMCs, and (c) observe proximity and engagement of A549 target cells by CD-8 positive PBMCs in immune-cell killing experimentsThis FabFluor-488/IncuCyte method enables long-term tracking and quantification of cell surface protein expression and the ability to identify cell subsets in living cultures over time. This method should prove powerful in analyses on complex and advanced heterogeneous cell models.
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Dreyer, Kirsten. "Om og af H. C. Andersen. H.C. Andersens samlede værker. Eventyr og Historier I-III. Udgivet af Det Danske Sprog- og Litteraturselskab." Fund og Forskning i Det Kongelige Biblioteks Samlinger 43 (January 1, 2004): 442. http://dx.doi.org/10.7146/fof.v43i1.40660.
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OM OG AF H. C. ANDERSEN ANDERSEN. H.C. Andersens samlede værker. Eventyr og Historier I-111. Udgivet af Det Danske Sprog- og Litteraturselskab under redaktion af Klaus P. Mortensen. Udgivere: Laurids Kristian Fahl, Esther Kielberg, Klaus P. Mortensen, Jesper Gehlert Nielsen under medvirken af Finn Gredal Jensen. Det Danske Sprog- og Litteraturselskab/ Gyldendal 2003. 560 s. + 528 s + 488 s. Jackie Wullschlager: H.C. Andersen. En biografi. På dansk ved Pia Juul. Hans Reitzels Forlag. 2002. 504 s. Jens Andersen: Andersen. En biografi 1-2. Gyldendal 2003. 528 s. + 440 s
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Stoldt, Volker R., Matthias Abtmeier, Khon C. Huynh, and Rudiger E. Scharf. "Platelets Can Attack Candida Albicans Hypha Opsonized by Fibronectin." Blood 118, no. 21 (November 18, 2011): 2210. http://dx.doi.org/10.1182/blood.v118.21.2210.2210.
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Abstract Abstract 2210 Background and objectives: Candida albicans is a dimorphic fungus that can change between its yeast and hypha form. The ability to germinate and grow in hypha is a relevant factor of virulence. Platelets can bridge innate and adaptive immunity. To attack microorganisms, platelets store microbicidal and immune stimulatory proteins in their α-granules. Platelet integrins and their plasma ligands can govern both binding to the pathogen and activation of platelets with subsequent secretion of their granular constituents. Fibronectin (Fn), a large dimeric glycoprotein, with specific binding sites for integrins and collagens, circulates in plasma and is part of extracellular matrix of the host. Both platelets and C. albicans are known to interact with Fn. Platelet can bind Fn specifically by their integrins α5β1, αIIbβ3, and αvβ3. Several protein species have been identified on the surface of C. albicans also interacting with Fn. Here, we examined whether or not Fn can support the platelet-mediated host defense against C. albicans. Material and methods: Platelet-rich plasma from citrated anticoagulated human blood was washed with phosphate-buffered saline (PBS), pH 6.5, containing 2 U/ml apyrase. The platelet pellet was carefully resuspended in Tyrode buffer, pH 7.3, containing 2 mM MgCl2, 2 mM CaCl2, and 10 μM of the cell tracker dye CMFDA (5-chloromethylfluorescein diacetate, Invitrogen). CMFDA did not affect platelet function, as documented by intact activation and normal adhesion, aggregation, secretion, and thromboxyne A2 formation. Washed and stained platelets were adjusted at a concentration of 2.5 × 108 plt/ml. Platelet aggregation was induced by 40 nM phorbol 12-myristate 13-acetate (PMA) or 10 μg/ml collagen and recorded by changes in light transmission. Fn was purified from human fresh-frozen plasma by gelatine sepharose affinity chromatography and subsequent elution with 3 M urea. The isolated adhesive protein was conjugated with alexa fluor 488 according the manufacturer's instruction (Invitrogen). C. albicans, cultured over night in yeast extract pepton glucose medium at 30°C, was washed twice in PBS, pH 7.3, and starved for 1 h at room temperature in PBS. C. albicans was diluted in PBS supplemented with fetal calf serum (10 %) to adjust a final optical density of 0.4 as a measure of hypha formation and to induce germination at 37°C. Samples were taken at various times of germination. For flow cytometric binding studies, serum was washed away and C. albicans yeast and hypha forms were incubated for 1h with 40 μg/ml Fn-alexa fluor 488 or 1×107 fluorescently CMFDA-stained platelets were added under static conditions. Results: C. albicans germination (mean + SD) at 30 min, 60 min, and 120 min of incubation revealed 5+4 %, 40+9 %, and 90+12 % hypha, as determined by light microscopy and confirmed by flow cytometry, as quantified by their characteristic forward and side scatter profiles. C. albicans hypha induction increased binding of Fn-alexa fluor 488 (40 μg/ml) from 1.2+0.3 % positive yeast cells (baseline), to 9.4+2.9 % at 30 min, 23.0+3.4 % at 60 min, or 45.0+5.1 % at 120 min, respectively (p<0.05, each). As observed by microscopy, C. albicans germination tubes promoted the binding Fn. Upon incubation, germation of C. albicans, obtained after 120 min, increased its binding to washed platelets 5-fold, as compared to the yeast form (p<0.05). Moreover, pretreatment of C. albicans hypha with Fn (40 μg/ml) enhanced their specific binding to platelet integrin αIIbβ3, as abciximab (2 μg/ml) blocked Fn binding to C. albicans completely. Conclusion: Hypha of C. albicans, a relevant factor of virulence, can be attacked by platelets. Fibronectin, a ligand of the β1 and β3 platelet integrins, opsonizes the hypha forms of C. albicans and supports platelet binding. The multiple binding sites in this adhesive protein for collagen and integrins can support the platelet-mediated host defense and may modify adaptive immune responses against C. albicans. Disclosures: No relevant conflicts of interest to declare.
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Dumitru, Alina Iulia, Rodica Mariana Ion, Florentina Marilena Clicinschi, Beatrice Gabriela Sbarcea, Alina Ruxandra Caramitu, and Georgeta Velciu. "The Influence of the Dopants on the Properties of Lead Zirconate Titanate System." Scientific Bulletin of Valahia University - Materials and Mechanics 19, no. 21 (October 1, 2023): 11–14. http://dx.doi.org/10.2478/bsmm-2023-0012.
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Abstract The doped Pb(Zr0.52Ti0.48)O3 system with Nb5+ and Sr2+ were obtained by solid state reactions. The influence of nature of dopants on structural and microstructural properties were analyzed by XRD and SEM techniques and were also investigated the obtained dielectric and piezoelectric properties. The XRD analyzes and the SEM images highlighted the obtaining of homogeneous tetragonal structures, of the perovskite type. The physical (ρa), dielectric constant (ɛr) and piezoelectric (kp) properties of the doped PZT systems were investigated. The Nb5+ doped Pb(Zr0.52Ti0.48)O3 system presented the ɛr = 488 and kp = 0,52. Sr2+ substitution for Nb5+ doped Pb(Zr0.52Ti0.48)O3 reduced the Curie temperature (from 420°C to 360°C), increased the dielectric constant at 1180, and also decreasing the kp (0,39).
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Shin, Hwangyu, Hyeonmi Kang, Jong-Hyung Kim, Yun-Fan Wang, Seungho Kim, Kwang-Yol Kay, and Jongwook Park. "Synthesis and Electroluminescence Property of New Hexaphenyl Benzene Derivatives Including Emitting Core for OLED." Journal of Nanoscience and Nanotechnology 15, no. 10 (October 1, 2015): 8289–94. http://dx.doi.org/10.1166/jnn.2015.11260.
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Three new emitting compounds of 5P-2TPA, 5P-2An and 5P-2Py for OLED based on hexaphenyl benzene moiety were synthesized. Physical properties were systematically examined by the change of the substitution groups of the synthesized materials. Photoluminescence (PL) spectrum of the synthesized materials showed maximum emitting wavelengths of about 437∼488 nm in solution state and 457∼516 nm in film state, indicating blue emission color. OLED devices were fabricated by the synthesized compounds using vacuum deposition process as an emitting layer. Device structure was ITO/2-TNATA 60 nm/NPB 15 nm/EML 35 nm/TPBi 20 nm/LiF 1 nm/Al 200 nm. External quantum efficiencies and CIE values of 5P-2TPA, 5P-2An and 5P-2Py were 3.34, 1.06 and 2.06% and (0.14, 0.12), (0.23, 0.45) and (0.24, 0.45), respectively. The three compounds exhibited thermal stablility with high Td of 426 °C, 449 °C and 467 °C.
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Meledin, V. G., S. V. Dvoinishnikov, I. K. Kabardin, A. S. Chubov, G. V. Bakakin, and A. K. Kabardin. "Development of the method of laser Doppler spectroscopy of nanoparticles in liquids and implementation of the method in the LAD-079 spectrometer." Journal of Physics: Conference Series 2057, no. 1 (October 1, 2021): 012094. http://dx.doi.org/10.1088/1742-6596/2057/1/012094.
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Abstract In this work, a method for physicochemical and biological optical studies of the nanoparticles size distribution is developed. Its implementation in the LAD-079 spectrometer is described. The distinctive features of the LAD-079 spectrometer include the following. Multi-angle parallel measurements of static and dynamic light scattering, scattering angle from 0–180 degrees. Probing at three wavelengths with the ability to analyze the polarization activity of the sample (488 nm, 532 nm, 650 nm). Programmable precision thermostat with an error less than 0.1 °C in the range 0 ÷ +80°C with the possibility of building and software implementation of the experiment plan. Robust monobloc design of the spectrometer that does not require adjustments and a special optical table. The ability to measure the size of dispersed nanoparticles in low-transparent liquids.
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KOLANOWSKA, MARTA, ALEXANDER HIRTZ, and DIEGO FRANCISCO TOBAR SUÁREZ. "Transfer of the Ecuadorian species Oncidium hernandezii to the genus Caucaea (Orchidaceae)." Phytotaxa 309, no. 3 (June 16, 2017): 299. http://dx.doi.org/10.11646/phytotaxa.309.3.16.
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The Neotropical genus Caucaea Schlechter (1920: 190) was established based on a small-flowered Colombian species, C. obscura (Lehm. & Krenzl. in Kränzlin 1899: 488) Schlechter (1920: 190) originally described as representative of Rodriguezia Ruiz López & Pavón (1794: 115, t. 25). In 1934, Mansfeld revealed that previously discovered Abola radiata Lindley (1853: 1) is a synonym of Schlechter’s C. obscura. Since the generic name Abola was used earlier by Adanson (1763) for a genus of Poaceae, he proposed to synonymize C. obscura with A. radiata under name Caucaea radiata (Lindl.) Mansfeld (1934: 343). Since this publication the genus had been considered monospecific until Williams et al. (2001) published surprising results of molecular research. According to their study, C. radiata is embedded in clade composed of representatives of Oncidium section Cucullata Kränzlin (1922: 128), which are large-flowered plants. In both C. radiata and Oncidium section Cucullata the lateral sepals are partially connate, the lip callus is rather simple and the gynostemium morphology is similar. The composition of Caucaea was later confirmed by Neubig et al. (2012).
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Hendrick, Erin, Margaret Frey, Erik Herz, and Ulrich Wiesner. "Cellulose Acetate Fibers with Fluorescing Nanoparticles for Anti-counterfeiting and pH-sensing Applications." Journal of Engineered Fibers and Fabrics 5, no. 1 (March 2010): 155892501000500. http://dx.doi.org/10.1177/155892501000500103.
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Fluorescent silica nanoparticles, Cornell dots (C dots), were incorporated into electrospun cellulose acetate (CA) fibers. Two types of C dots were used in this study. The first type was comprised of a fluorescent dye-containing silica core surrounded by a silica shell. These nanoparticles fluoresce at 572 nm when exposed to 541 nm light. Increasing C dot loading in the spinning dope above 10% w/w did not result in an increase in C dot content within the final fibers. Scanning electron microscopy indicated that the nanoparticle incorporation had very little effect on the fiber morphology. The mechanical properties of the electrospun fabrics were not negatively affected by C dot addition, even though final loading constituted nearly one-third of the weight of the fibers. A second type of C dots, with both a fluorescent core and a pH-sensitive shell, were also incorporated in CA fibers. These C dots fluoresce at both 572 nm as described above, and at 518 nm, when exposed to 488 nm light. Fluorescence intensity at 541 nm increased with increasing pH. For both nanoparticle-incorporated fabrics, the resulting fibers are white under ambient lighting, and fluoresce at their given wavelengths of light.
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Finney, Hazel, David J. Newman, Walter Gruber, Peter Merle, and Christopher P. Price. "Initial evaluation of cystatin C measurement by particle-enhanced immunonephelometry on the Behring nephelometer systems (BNA, BN II)." Clinical Chemistry 43, no. 6 (June 1, 1997): 1016–22. http://dx.doi.org/10.1093/clinchem/43.6.1016.
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Abstract Serum cystatin C has been suggested as a new marker of glomerular filtration rate (GFR). We describe a fully automated and rapid particle-enhanced nephelometric immunoassay (PENIA) for measuring serum cystatin C on the Behring nephelometer systems (BNA, BN II). Each sample is analyzed in 6 min with as many as 75 samples per batch. The assay covers the range 0.23–7.25 mg/L, up to seven times the upper limit of normal. The intra- and interassay imprecision are <3.3% and <4.5%, respectively. There is absolute linearity across the assay range (r2 = 0.997), with analytical recovery by cystatin C addition between 95% and 109% (mean 102%). Hemoglobin (≤8.0 g/L), bilirubin (≤488 μL), triglycerides (≤23 mmol/L), rheumatoid factor (≤2000 kIU/L), and myeloma paraprotein (≤41 g/L) do not interfere with the assay. This assay agreed well with an in-house particle-enhanced turbidimetric immunoassay (PETIA) (mean difference = 1.73 ± 2.10) and a commercial PETIA (mean difference = 1.13 ± 0.86). This is a new assay by which cystatin C may be effectively used as a marker of GFR estimation.
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Guo, Li Na, and Sha Ren. "Preparation and Luminescent Properties of Tb3+ Doped Bi2WO6 Phosphor." Applied Mechanics and Materials 851 (August 2016): 72–77. http://dx.doi.org/10.4028/www.scientific.net/amm.851.72.
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The Tb3+doped Bi2WO6 luminescent materials are synthesized by a co-precipitation method. The structure and composition of the samples were characterized by DTA-TG and XRD. The results showed that the formula of the sample is Bi2WO6 at the temperature of 600°C~900°C, which belongs to the needed and stable orthorhombic ctystal. The luminescence properties were characterized by UV-vis and fluorescence spectrophotometer. The results showed that, the phosphor can be effectively stimulated by blue light of 488 nm, we can get the intense green-emission, which corresponds to the 5D4→7F5 transitions of Tb3+. It indicates that the samples match the light-emitting wavelength of the common blue-emitting InGaN chips and are expected to be applicable to a new type of green-emission light conversion materials which are applicable to the blue LED chips.
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Kovacevic, Nadica, Radoslava Doder, Tomislav Preveden, and Maria Pete. "Risk factors for recurrent Clostridium difficile infection among patients in the Clinical Centre of Vojvodina, Serbia: A retrospective clinical trial." Vojnosanitetski pregled 76, no. 4 (2019): 392–97. http://dx.doi.org/10.2298/vsp170321119k.
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Background/Aim. In the last two decades the incidence of recurrent Clostridium difficile infection (CDI) has risen. The aim of this study was to determine the risk factors for the recurrent CDI among patients hospitalized with the initial CDI. Methods. We conducted a retrospective clinical trial at the Clinic for Infectious Diseases, Clinical Center of Vojvodina, Serbia, between January 2010 and January 2016. We enrolled 488 patients with the initial CDI who were treated with oral vancomycin (125 mg, 4 times per day) or oral metronidazole (400 mg, 3 times per day) for 10 days. After the completion of therapy, there was 60 days of the follow-up period for the assessment of the rates of relapse. To determine the risk factors for the CDI relapse, we compared the demographics, clinical and laboratory characteristics of the patients who had a relapse with the patients who had a stable clinical response. Results. Of the 488 cases, 29.09% recurred. The relapse occured in 22.72% patients who received vancomycin and in 36.60% patients treated with metronidazole (p = 0.038). A statistically significant effect on the CDI relapse had the comorbidities such as a malignancies (19.52% vs 8.82%, p = 0.023) and the postoperative CDI (25.67% vs 10.29%, p = 0.035), hipoalbuminemia (< 25 g/L) (70.27% vs 41.94%; p = 0.034) and the concomitant antibiotic therapy (50.67% vs 20.29%; p = 0.031). The persistence of C. difficile toxin in the stool at the end of treatment was registered in 22.32% of patients treated with metronidazole vs 9.09% of patients given vancomycin (p = 0.03). Conclusion. Our data suggest that the important risk factors for the CDI relapse are comorbidities (surgery within a month before developing CDI and malignancy), hipoalbuminemia (< 25g/L) and concomitant non-CDI antibiotics therapy. Vancomycin is more effective than metronidazole in the elimination of C. difficile toxins. The presence of C. difficile toxins in the stool after the successful completion of the initial CDI therapy does not affect significantly the occurrence of relapse.
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Herault, Olivier, and Christine Vignon. "Flow Cytometric Quantification of All Cell Cycle Phases and Apoptosis in a Two-Color Fluorescence Plot." Blood 120, no. 21 (November 16, 2012): 4720. http://dx.doi.org/10.1182/blood.v120.21.4720.4720.
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Abstract Abstract 4720 An optimal technology for cell cycle analysis would allow measuring concomitantly apoptosis, G0, G1, S, G2 and M phases in combination with cell surface phenotyping. We propose an easy method in flow cytometry allowing this discrimination in an only two-color fluorescent plot. It is based on the concomitant use of 7-amino-actinomycin D and antibodies anti-Ki67 and anti-phospho(Ser10)-histone H3 conjugated to Alexa Fluor.. 488 to discriminate G0 et M phases, respectively. The proposed method is particularly valuable in a clinical setting as verified by analyzing human leukemic cells from marrow samples or exposed to cell cycle modifiers. The method was established using the human KG1a cell line and was applied to charcterize the cell cycle and apoptosis of fresh human marrow leukemic cells. The cells were permeabilized with 1 mL of ice cold ethanol (1 h, 4°C). Following two washes with PBS, 1% FBS and 0.25% Triton X-100 (PFT), the cells were stained in 200 μL of PFT for 30 min at room temperature in the dark with 1 μg 7-AAD (Sigma-Aldrich), 5 μL Alexa Fluor.. 488-conjugated anti-human Ki67 mAb (B56, Becton-Dickinson) and 3 μL Alexa Fluor.. 488-conjugated anti-phospho(ser10)-histone H3 polyclonal antibody (Cell Signaling Technology). A control tube was prepared with 1 μg 7-AAD and 5 μL of mouse IgG1 Alexa Fluor.. 488 (Becton-Dickinson). After 2 washes with PFT, the cells were stained with 10 μL of APC-Cy7-conjugated anti-CD45 (A20, Becton-Dickinson) followed by incubation for 20 min at 4°C. Cells were then washed twice with PBS, centrifuged for 5 min at 500 g and resuspended in 300 μL of PBS. This flow cytometric method allows for a precise analysis of the impact on the cell cycle of various functional modulators. As an example, it was applied to analyze the pro-quiescent effects of contact with bone marrow MSCs as well as and the apoptosis induction and mitosis inhibition of the human KG1a leukemic cell line by camptothecin. The cell cycle characteristics of untreated KG1a cells were clearly quantified as follows: 0.4% sub-G1, 0.8% G0, 67.9% G1, 14.9% S, 14.2% G2 and 1.8% M phase. The contact with marrow MSCs during 72 h induced an increase in G0 phase and a decrease in M phase (5.3% and 0.4%, respectively). We verified the anti-proliferative and pro-apoptotic effects of 24 h exposure to camptothecin, which induced a decrease in S, G2 and M phases (6.1%, 6.2% and 0.4%, respectively) and an increase in sub-G1 phase (1.7%). Moreover, it is interesting to note that the staining protocol preserved the integrity of the plasma membrane and allows for the analysis of heterogeneous cell populations. We document here the successful utilization of this method to discriminate concomitantly apoptosis and the cell cycle phases in a model of leukemic cells exposed to inducers of cell cycle perturbations. The value of this method to analyze heterogeneous populations was shown by discriminating the marrow cells from acute myeloid leukemia. Disclosures: No relevant conflicts of interest to declare.
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Carlsen, Toke Meier, and Eun Ji Kang. "Corrigendum to “Gauge-invariant ideals of C⁎-algebras of Boolean dynamical systems” [J. Math. Anal. Appl. 488 (1) (2020) 124037]." Journal of Mathematical Analysis and Applications 495, no. 2 (March 2021): 124754. http://dx.doi.org/10.1016/j.jmaa.2020.124754.
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Yi, Chao Jue, Peng Cheng Zhai, Li Zhou Dong, and Qi Hao Fu. "Research on the Strength Improvement of 7A04 Aluminum Alloy." Advanced Materials Research 488-489 (March 2012): 19–21. http://dx.doi.org/10.4028/www.scientific.net/amr.488-489.19.
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By using cryogenic treatment on 7A04 aluminum alloy tested with micrographic analysis and mechanical properties test, we studied the impact on mechanical properties of 7A04 aluminum alloy The results showed that the strength of 7A04 aluminum alloy could be highly increased and the grain size would be reduced in the process through being treated in 480°C/80min + aging in 120°C/4h + cryogenic treatment + aging at 120°C/16h.7A04 aluminum alloy are more fully recrystallized to grain refinement and the tensile strength of it can be increased to 720Mpa after the treatment.
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Kurbanov, Yu K. "First data on the occurence and ecology of Giant hookear sculpin <i>Artediellus ingens</i> (Cottidae) off the Kuril Islands." Researches of the aquatic biological resources of Kamchatka and the North-West Part of the Pacific Ocean, no. 70 (January 13, 2024): 63–69. http://dx.doi.org/10.15853/2072-8212.2023.70.63-69.
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Based on materials collected during monitoring of bottom trawl fishing, the occurrence, some aspects of ecology and size composition of giant hookear sculpin Artediellus ingens were examined for the first time off the Middle Kuril Islands. It has been established that this species is a regular bycatch at the depths 250–488 m at the temperature of the near-bottom water layer of 1.8–3.9 °C. Most likely A. ingens belongs to mesobenthal ichthyocene. In the trawl catches, this species is represented by individuals of 10–19 cm TL. Comparison of the maximum body length with that of the other species of the genera Artediellus indicated this species is one of the largest.
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Permanasari, Ayu Ratna. "The Pyrolysis Reactor Design and The Effect of Liquid Smoke from Coconut Shell on Microbial Contamination of Tofu." Current Journal: International Journal Applied Technology Research 1, no. 2 (October 1, 2020): 128–39. http://dx.doi.org/10.35313/ijatr.v1i2.28.
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Liquid smoke is a natural food preservative which can be made of coconut shells through the pyrolysis process. This study aimed to design a pyrolysis reactor and utilize the coconut shell waste to produce liquid smoke as a natural preservative of tofu. 1.5 kg of chopped coconut shell was pyrolyzed at 400C for 5 hours and produced 488 mL of grade 3 liquid smoke with a yield of 34.23%. The liquid smoke was then purified by extraction using ethyl acetate (1: 1 ratio) solvent and 70C temperature for 2 hours. The extract was then distilled at 80C and produced grade 1 liquid smoke. This liquid smoke had an acid content of 12.26% and a phenol content of 0.73%. This liquid smoke was then applied to tofu for 3 days and analyzed the microbial contamination. The smallest amount of microbial contamination was found in the samples of yellow tofu and white tofu coated with liquid smoke and stored in the refrigerator for 1.4 × 105 CFU / mL and 8 × 103 CFU/ml.
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Maiwald, Martin, Heinar Schmidt, Bernd Sumpf, Reiner Güther, Götz Erbert, Heinz-Detlef Kronfeldt, and Günther Tränkle. "Microsystem Light Source at 488 nm for Shifted Excitation Resonance Raman Difference Spectroscopy." Applied Spectroscopy 63, no. 11 (November 2009): 1283–87. http://dx.doi.org/10.1366/000370209789806803.
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A microsystem light source emitting at 488 nm was tested and applied as a light source for shifted excitation resonance Raman difference spectroscopy (SERRDS). A nonlinear frequency conversion using a distributed feedback (DFB) diode laser emission at 976 nm and a periodically poled lithium niobate (PPLN) waveguide crystal was realized on a micro-optical bench with a footprint of 25 mm × 5 mm. Joint temperature management via the microbench is used for wavelength tuning. Two emission lines at 487.61 nm and 487.91 nm are used for the SERRDS experiments. The Raman spectra of the test sample polystyrene demonstrate that a laser bandpass filter did not need to be implemented. Resonance Raman spectra of Tartrazine (FD&C Yellow 5, E 102) in distilled water are presented to demonstrate the suitability of this light source for SERRDS in, e.g., food safety control.
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Hassan, Naveed, Manickam Minakshi, Willey Yun Hsien Liew, Amun Amri, and Zhong-Tao Jiang. "Thermal Characterization of Binary Calcium-Lithium Chloride Salts for Thermal Energy Storage at High Temperature." Energies 16, no. 12 (June 14, 2023): 4715. http://dx.doi.org/10.3390/en16124715.
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Due to their excellent thermophysical properties and high stability, inorganic salts and Forsalt mixtures are considered promising thermal energy storage materials for applications operating at high temperatures. A mixture of binary salts, such as CaCl2 (58 wt.%)-LiCl (42 wt.%), was investigated in this work to understand their thermal properties and stability for use in TES systems. Thermophysical properties, such as onset melting and crystallization temperature, enthalpy of fusion, and crystallization enthalpy, were all investigated experimentally via the use of a simultaneous thermal analyzer. The experimental findings demonstrated a suitable onset melting temperature of 488 °C and a solidification temperature of 480 °C. The heat of fusion was observed as 206 J/g, whereas the heat of crystallization was recorded as 180 J/g. Thermal repeatability tests indicated little variations in melting temperature; however, fusion enthalpies changed significantly over the course of 30 heating-cooling cycles. Additionally, the results obtained from the thermogravimetric analysis showed relatively weak thermal stability with considerable mass changes. This might be caused by the salts decomposing at elevated temperatures. In order to validate this, a high-temperature in-situ X-ray diffraction technique was used to verify the thermal instability of the binary salt mixture with and without thermal cycling. The thermal decomposition of parent salts and the subsequent formation of new phases with the formation of voids were shown to be the cause of thermal instability. It is concluded that the binary mixture of chloride salt showed suitable thermal properties but relatively weak thermal stability, which may limit its use in practical applications.
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Gao, Lin-Bo, Li-Juan Li, Xin-Min Pan, Zhao-Hui Li, Wei-Bo Liang, Peng Bai, Yin-Hua Zhu, and Lin Zhang. "A genetic variant in the promoter region of miR-34b/c is associated with a reduced risk of colorectal cancer." Biological Chemistry 394, no. 3 (March 1, 2013): 415–20. http://dx.doi.org/10.1515/hsz-2012-0297.
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Abstract The miR-34 family members, described as potential tumor suppressors, were downregulated in colorectal cancer (CRC). Loss of miR-34 impairs TP53-mediated cell death, while overexpression of miR-34 induces apoptosis. A potentially functional polymorphism (i.e., rs4938723T/C) in the promoter region of pri-miR-34b/c was predicted to influence the GATA-X binding sites. We aimed to investigate the association between miR-34b/c rs4938723 and TP53 Arg72Pro polymorphisms and the risk of CRC. We genotyped the two polymorphisms in 347 CRC patients and 488 healthy controls using polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing assay. We found that the CC genotype and C allele of the miR-34b/c rs4938723 were associated with a significantly decreased risk of CRC compared with the TT genotype and T allele (CC vs. TT: adjusted OR=0.56; 95% CI, 0.34–0.91; C vs. T: adjusted OR=0.78; 95% CI, 0.64–0.97). In combined analysis, a borderline significance was also observed in subjects carrying the rs4938723 CT/CC and TP53 GG genotypes (adjusted OR=0.66; 95% CI, 0.43–0.99). These findings indicate that the rs4938723 in the promoter region of pri-miR-34b/c was a protective factor for the development of CRC. As the significance is marginal, further replication studies are warranted to confirm these results.
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Chamberlain, Alanna M., Aaron R. Folsom, Susan R. Heckbert, Wayne D. Rosamond, and Mary Cushman. "High-density lipoprotein cholesterol and venous thromboembolism in the Longitudinal Investigation of Thromboembolism Etiology (LITE)." Blood 112, no. 7 (October 1, 2008): 2675–80. http://dx.doi.org/10.1182/blood-2008-05-157412.
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AbstractWe determined prospectively the risk of venous thromboembolism (VTE) in relation to baseline high-density lipoprotein cholesterol (HDL-c) in 19 049 participants of the Longitudinal Investigation of Thromboembolism Etiology (LITE), which was composed of 14 490 participants of the Atherosclerosis Risk in Communities (ARIC) study and 4559 participants of the Cardiovascular Health Study (CHS). In addition, we determined the risk of VTE in relation to baseline subfractions of HDL (HDL2 and HDL3) and apolipoprotein A-I (apoA-I) in 14 488 participants of the ARIC study. Age-adjusted incidence rates of VTE by HDL-c quartile ranged from 1.64 to 1.91 per 1000 person-years in men and 1.40 to 1.94 per 1000 person-years in women; however, there was no apparent trend of VTE incidence across HDL-c quartiles for either sex. The multivariate adjusted hazard ratios of VTE by HDL-c quartiles (with quartile 4 as the reference) were nonsignificant for both sexes and ranged between 0.91 and 0.99 for men and 0.78 and 1.22 for women. Results did not differ in separate evaluations of idiopathic and secondary VTE. In the ARIC study, there was no trend of VTE hazard ratios across quartiles of HDL2, HDL3, or apoA-I. Low HDL-c does not appear to be an important VTE risk factor.
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Jia, Xianbo, Jichen Chen, Chenqiang Lin, and Xinjian Lin. "Cloning, Expression, and Characterization of a Novel Thermophilic Monofunctional Catalase fromGeobacillussp. CHB1." BioMed Research International 2016 (2016): 1–8. http://dx.doi.org/10.1155/2016/7535604.
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Catalases are widely used in many scientific areas. A catalase gene (Kat) fromGeobacillussp. CHB1 encoding a monofunctional catalase was cloned and recombinant expressed inEscherichia coli(E. coli), which was the first time to clone and express this type of catalase ofgenus Geobacillusstrains as far as we know. ThisKatgene was 1,467 bp in length and encoded a catalase with 488 amino acid residuals, which is only 81% similar to the previously studiedBacillussp. catalase in terms of amino acid sequence. Recombinant catalase was highly soluble inE. coliand made up 30% of the totalE. coliprotein. Fermentation broth of the recombinantE. colishowed a high catalase activity level up to 35,831 U/mL which was only lower than recombinantBacillussp. WSHDZ-01 among the reported catalase production strains. The purified recombinant catalase had a specific activity of 40,526 U/mg andKmof 51.1 mM. The optimal reaction temperature of this recombinant enzyme was 60°C to 70°C, and it exhibited high activity over a wide range of reaction temperatures, ranging from 10°C to 90°C. The enzyme retained 94.7% of its residual activity after incubation at 60°C for 1 hour. High yield and excellent thermophilic properties are valuable features for this catalase in industrial applications.
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Fu, M., P. W. Crous, Q. Bai, P. F. Zhang, J. Xiang, Y. S. Guo, F. F. Zhao, et al. "Colletotrichum species associated with anthracnose of Pyrus spp. in China." Persoonia - Molecular Phylogeny and Evolution of Fungi 42, no. 1 (July 19, 2019): 1–35. http://dx.doi.org/10.3767/persoonia.2019.42.01.
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Colletotrichum species are plant pathogens, saprobes, and endophytes on a range of economically important hosts. However, the species occurring on pear remain largely unresolved. To determine the morphology, phylogeny and biology of Colletotrichum species associated with Pyrus plants, a total of 295 samples were collected from cultivated pear species (including P. pyrifolia, P. bretschneideri, and P. communis) from seven major pear-cultivation provinces in China. The pear leaves and fruits affected by anthracnose were sampled and subjected to fungus isolation, resulting in a total of 488 Colletotrichum isolates. Phylogenetic analyses based on six loci (ACT, TUB2, CAL, CHS-1, GAPDH, and ITS) coupled with morphology of 90 representative isolates revealed that they belong to 10 known Colletotrichum species, including C. aenigma, C. citricola, C. conoides, C. fioriniae, C. fructicola, C. gloeosporioides, C. karstii, C. plurivorum, C. siamense, C. wuxiense, and two novel species, described here as C. jinshuiense and C. pyrifoliae. Of these, C. fructicola was the most dominant, occurring on P. pyrifolia and P. bretschneideri in all surveyed provinces except in Shandong, where C. siamense was dominant. In contrast, only C. siamense and C. fioriniae were isolated from P. communis, with the former being dominant. In order to prove Koch's postulates, pathogenicity tests on pear leaves and fruits revealed a broad diversity in pathogenicity and aggressiveness among the species and isolates, of which C. citricola, C. jinshuiense, C. pyrifoliae, and C. conoides appeared to be organ-specific on either leaves or fruits. This study also represents the first reports of C. citricola, C. conoides, C. karstii, C. plurivorum, C. siamense, and C. wuxiense causing anthracnose on pear.
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Tian, Qing Chao, Xian Ping Dong, Hai Chao Cui, and Ke Xu. "Characterization of the Welded-Joint of High-Strength High-Toughness Seamless Steel Pipe under High-Cycle Fatigue Condition." Materials Science Forum 896 (March 2017): 202–8. http://dx.doi.org/10.4028/www.scientific.net/msf.896.202.
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The welded joint of a S890QL grade steel pipes containing 1.2% Ni have been prepared to characterize the use performance under high cycle fatigue test. It has been found that the fatigue strength of the welded joint is 290MPa with a fatigue life of more than 10 million cycles, and the obtained Basquin equation is σa=488*(2N)-0.02758 . It is found that the steel exhibits the whole bainite microstructure when the cooling rate is less than 1°C/s. The welded joint is divided into the weld zone, the coarse grain zone, the fine grain zone, the softening zone and the matrix. The fine grain characteristic in the welded area determines the good anti fatigue performance of the investigated steel.
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Chen, Sinn-wen, Hsiu-feng Hsu, and Chih-wei Lin. "Liquidus projection of the ternary Ag–Sn–Ni system." Journal of Materials Research 19, no. 8 (August 2004): 2262–67. http://dx.doi.org/10.1557/jmr.2004.0295.
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The liquidus projection of the ternary Sn–Ag–Ni system at the Sn-rich side was determined experimentally. No ternary compound was found, and the ζ–Ag4Sn, Ag3Sn, and Sn existed as the primary solidification phases only in very small compositional portions of the ternary Sn–Ag–Ni system. In more than half of the compositional regime of the ternary system, the Ni3Sn2 phase was the primary solidification phase. The differential thermal analysis technique was used to determine the reaction temperatures and solidification sequences of various Sn-rich Sn–Ag–Ni alloys. Three invariant reactions were found: L = Sn + Ni3Sn4 + Ag3Sn, L + ζ–Ag4Sn = Ni3Sn4 + Ag3Sn and L + Ni3Sn2 = ζ–Ag4Sn + Ni3Sn4. Their reaction temperatures have been determined to be 219, 488, and 516.5 °C, respectively.
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Price, D. A., M. L. Schmid, K. Agarwal, M. Hewett, W. L. Craig, A. D. Burt, and M. F. Bassendine. "P.488 Chronic hepatitis C in injecting drug users: a comparison with non-injecting drug users in the same geographic area." Journal of Clinical Virology 36 (January 2006): S212. http://dx.doi.org/10.1016/s1386-6532(06)80661-x.
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Rossi, Davide, Silvia Rasi, Valeria Spina, Alessio Bruscaggin, Sara Monti, Carmela Ciardullo, Hossein Khiabanian, et al. "Integrated Mutational and Cytogenetic Analysis Identifies New Prognostic Subgroups in Chronic Lymphocytic Leukemia." Blood 120, no. 21 (November 16, 2012): 712. http://dx.doi.org/10.1182/blood.v120.21.712.712.
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Abstract Abstract 712 The identification of NOTCH1, SF3B1, MYD88 and BIRC3 genetic lesions in chronic lymphocytic leukemia (CLL) prompts a comprehensive and dynamic prognostic algorithm including gene mutations, chromosomal abnormalities, and their changes during clonal evolution. The study utilized both time-fixed (637 newly diagnosed CLL) and time-dependent (257 CLL provided with 524 sequential samples) approaches. Each sample was investigated for TP53, NOTCH1, SF3B1, MYD88, and BIRC3 mutations by Sanger sequencing and for 17p13, 11q22-q23, 13q14 and BIRC3 deletions and +12 by FISH. Del13q14 and +12 distributed in a mutually exclusive fashion (p<0.0001), and identified three main genetic subgroups: cases harboring del13q14, cases harboring +12 and cases lacking both del13q14 and +12. With the sole exception of the expected association between NOTCH1 mutations and +12 CLL (p=0.0014), the prevalence of the other genetic lesions did not differ among molecular subgroups. FISH abnormalities segregated patients in distinct prognostic groups according to Döhner (Fig 1A). Among new genetic lesions, survival analysis confirmed the independent prognostic value of NOTCH1, SF3B1 and BIRC3 lesions in this study cohort. MYD88 mutations had no prognostic effect (p=0.1728). Recursive partitioning analysis followed by random survival forest validation established the hierarchical order of relevance of the genetic lesions, and created an integrated mutational and cytogenetic (MUCY) model that classified newly diagnosed CLL into four prognostic subgroups (Fig 1B). High risk patients harbored TP53 disruption and/or BIRC3 disruption independent of co-occurring lesions (10-year survival: 29.1%). When the demographic effects of age, sex and year of diagnosis were compensated, the 10-year life expectancy of high risk patients was only 37.7% of that expected in the matched general population (p<0.0001). Intermediate risk patients harbored NOTCH1 and/or SF3B1 mutations and/or del11q22-q23 in the absence of TP53 and BIRC3 abnormalities (10-year OS: 37.1%). The 10-year life expectancy of intermediate risk patients was reduced to 48.5% compared to the matched general population (p<0.0001). The low risk category comprised both patients harboring +12 and patients wild type for all genetic lesions (i.e. normal) (10-year OS: 57.3%), with a 10-year life expectancy of 70.7% compared to the matched general population (p<0.0001). Very low risk patients harbored del13q14 as the sole genetic lesion (10-year OS: 69.3%), with a 10-year life expectancy only slightly (84.2%) and not significantly (p=0.1455) lower than that expected in the matched general population. Multivariate analysis selected the MUCY model as one of the most important independent risk factor of CLL OS (HR: 1.38; 95% CI: 1.18–1.60; p<0.0001; 99% bootstrap selection), along with age (HR: 1.06; 95% CI: 1.04–1.07; p<0.0001; 100% bootstrap selection), Rai stage (HR: 1.36; 95% CI: 1.23-1-51; p<0.0001; 100% bootstrap selection) and unmutated IGHV genes (HR: 1.63; 95% CI: 1.17–2.26; p=0.0039; 92% bootstrap selection). Overall, 21.5% (105/488) low risk patients according to the FISH model (del13q14, normal and +12) were reclassified into high risk genetic subgroups by the MUCY model because of the co-occurrence of NOTCH1 (64/488, 13.1%), SF3B1 (35/488, 7.1%), and TP53 (17/488, 3.4%) mutations or BIRC3 disruption (14/488, 2.8%). Consistently, the inclusion of NOTCH1, SF3B1 and BIRC3 lesions in addition to FISH abnormalities significantly improved the model accuracy of OS prediction (c-index: 0.617 vs c-index: 0.642 p<0.0001). At 10 years from diagnosis, 24.5% CLL of the very low and low risk genetic subgroups developed new TP53, NOTCH1, SF3B1, BIRC3 or del11q22-q23 lesions due to clonal evolution, and therefore switched to a higher risk category of the MUCY model. By time-dependent and landmark analysis, the MUCY model retained a statistically significant impact on CLL OS (HR: 1.52; 95% CI: 1.21–1.90; p=0.0003) at any time from diagnosis and independent of its dynamic changes due to clonal evolution. The MUCY model classifies CLL patients into more precise subgroups, advances our understanding of CLL biology, and improves current prognostic algorithms. These findings have relevant implications for the design of clinical trials aimed at assessing the use of mutational profiling to inform therapeutic decisions based on risk stratification. Disclosures: No relevant conflicts of interest to declare.
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Liu, Szu-Heng, Shih-Fang Huang, Yuan-Ling Hsu, Szu-Hua Pan, Yen-Ju Chen, and Yi-Hung Lin. "Structure of human collapsin response mediator protein 1: a possible role of its C-terminal tail." Acta Crystallographica Section F Structural Biology Communications 71, no. 8 (July 28, 2015): 938–45. http://dx.doi.org/10.1107/s2053230x15009243.
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Collapsin response mediator protein 1 (CRMP-1) is the first identified member of the CRMP family and is crucial for both the mediation of neuronal differentiation and in suppressing the invasion of lung cancer. The crystal structure of full-length human CRMP-1 was determined at a resolution of 3 Å. Human CRMP-1 comprises a tetrameric assembly; its overall structure is similar to that of mouse CRMP-1, but the measured electron density of the C-terminal residues 488–496 show a randomly coiled link that connects the protomers to each other, within which residues 497–572 are proteolytically susceptiblein vivo. Deletion of residues 472–572 by thrombinin vitronot only releases a randomly coiled tail but also transduces observable structural changes of CRMP-1, as revealed by analytical size-exclusive chromatography and circular dichroism spectra. These results indicate a possible alternative role in CRMP dynamics and function.
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O'Connell, T. D., J. E. Berry, A. K. Jarvis, M. J. Somerman, and R. U. Simpson. "1,25-Dihydroxyvitamin D3 regulation of cardiac myocyte proliferation and hypertrophy." American Journal of Physiology-Heart and Circulatory Physiology 272, no. 4 (April 1, 1997): H1751—H1758. http://dx.doi.org/10.1152/ajpheart.1997.272.4.h1751.
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We previously demonstrated that 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] inhibits myocyte maturation (T. D. O'Connell, D. A. Giacherio, A. K. Jarvis, and R. U. Simpson. Endocrinology 136: 482-488, 1995). To define further the role of 1,25(OH)2D3 in regulating myocardial development, we examined the effects of 1,25(OH)2D3 on proliferation and growth of primary cultures of ventricular myocytes isolated from neonatal rat hearts. When neonatal myocytes were grown in a serum-supplemented medium, cell number approximately doubled, and treating these myocytes with 1,25(OH)2D3 inhibited their proliferation by 56.56% after 4 days. Flow cytometry revealed that 1,25(OH)2D3 reduced the percentage of cells in the S phase of the cell cycle by 31.39% after 4 days. We show for the first time that proliferating cell nuclear antigen protein levels were specifically reduced by 1,25(OH)2D3. Protooncogene c-myc protein levels were also reduced by this hormone. Interestingly, a phorbol ester had a similar effect on myocyte proliferation. Furthermore, 1,25(OH)2D3 increased myocyte protein levels and increased cell size, suggesting that it induces cardiac myocyte hypertrophy. Our findings indicate that 1,25(OH)2D3 and phorbol esters directly regulate myocyte proliferation and induce myocyte hypertrophy. Finally, the data demonstrate that the mechanism by which 1,25(OH)2D3 regulates myocyte proliferation involves blocking entry into the S phase of the cell cycle.
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Yang, Junqiang, Zhongmei Shen, Pengyan Qu, Rui Yang, Anping Shao, Hao Li, Ailing Zhao, and Chunzhen Cheng. "Influences of Jujube Witches’ Broom (JWB) Phytoplasma Infection and Oxytetracycline Hydrochloride Treatment on the Gene Expression Profiling in Jujube." International Journal of Molecular Sciences 24, no. 12 (June 18, 2023): 10313. http://dx.doi.org/10.3390/ijms241210313.
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Jujube witches’ broom disease (JWB), caused by Candidatus Phytoplasma ziziphi, is the most destructive phytoplasma disease threatening the jujube industry. Tetracycline derivatives treatments have been validated to be capable of recovering jujube trees from phytoplasma infection. In this study, we reported that oxytetracycline hydrochloride (OTC-HCl) trunk injection treatment could recover more than 86% of mild JWB-diseased trees. In order to explore the underlying molecular mechanism, comparative transcriptomic analysis of healthy control (C group), JWB-diseased (D group) and OTC-HCl treated JWB-diseased (T group) jujube leaves was performed. In total, 755 differentially expressed genes (DEGs), including 488 in ‘C vs. D’, 345 in ‘D vs. T’ and 94 in ‘C vs. T’, were identified. Gene enrichment analysis revealed that these DEGs were mainly involved in DNA and RNA metabolisms, signaling, photosynthesis, plant hormone metabolism and transduction, primary and secondary metabolisms, their transportations, etc. Notably, most of the DEGs identified in ‘C vs. D’ displayed adverse change patterns in ‘D vs. T’, suggesting that the expression of these genes was restored after OTC-HCl treatment. Our study revealed the influences of JWB phytoplasma infection and OTC-HCl treatment on gene expression profiling in jujube and would be helpful for understanding the chemotherapy effects of OTC-HCl on JWB-diseased jujube.
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Mohr, Jeffrey R. "Movements of the timber rattlesnake (Crotalus horridus) in the South Carolina mountains." Bulletin of the Florida Museum of Natural History 51, no. 4 (August 10, 2012): 269–78. http://dx.doi.org/10.58782/flmnh.hznt3295.
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Although the timber rattlesnake (Crotalus horridus) is the most common rattlesnake in the eastern United States, populations have declined and only scattered metapopulations remain in what was once a large and extensive North American range. Whereas some C. horridus populations in forest communities of the northeastern and western US have been studied, information on those occurring along the southern part of its range is virtually non-existent. In South Carolina there has been relatively little research done on this species and there has been no formal study on C. horridus in the mountainous regions of the state. From 2006 to 2009, I radio-tracked several C. horridus in Table Rock State Park, South Carolina and documented their movement patterns. For the duration of the study, males moved a mean annual distance (± SE) of 3,047 ± 488 m, non-gravid females moved a mean (± SE) of 1,688 ± 517 m, and gravid females moved a mean (± SE) of 2,248 ± 597 m. Although mean distances moved were not statistically significant among groups in this study, mean distances travelled for all sexes were much shorter than observed in other populations. I hypothesize that forest management involving natural regeneration and fire suppression, and prey availability may influence C. horridus movements in Table Rock State Park.