Journal articles on the topic 'Bromine Physiological effect'
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1
Leedham Elvidge, E. C., S. M. Phang, W. T. Sturges, and G. Malin. "The effect of desiccation on the emission of volatile bromocarbons from two common temperate macroalgae." Biogeosciences 12, no. 2 (January 21, 2015): 387–98. http://dx.doi.org/10.5194/bg-12-387-2015.
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Abstract. Exposure of intertidal macroalgae during low tide has been linked to the emission of a variety of atmospherically-important trace gases into the coastal atmosphere. In recent years, several studies have investigated the role of inorganic iodine and organoiodides as antioxidants and their emission during exposure to combat oxidative stress, yet the role of organic bromine species during desiccation is less well understood. In this study the emission of dibromomethane (CH2Br2) and bromoform (CHBr3) during exposure and desiccation of two common temperate macroalgae, Fucus vesiculosus and Ulva intestinalis, is reported. Determination of the impact exposure may have on algal physiological processes is difficult as intertidal species are adapted to desiccation and may undergo varying degrees of desiccation before their physiology is affected. For this reason we include comparisons between photosynthetic capacity (Fv/Fm) and halocarbon emissions during a desiccation time series. In addition, the role of rewetting with freshwater to simulate exposure to rain was also investigated. Our results show that an immediate flux of bromocarbons occurs upon exposure, followed by a decline in bromocarbon emissions. We suggest that this immediate bromocarbon pulse may be linked to volatilisation or emissions of existing bromocarbon stores from the algal surface rather than the production of bromocarbons as an antioxidant response.
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Leedham Elvidge, E. C., S. M. Phang, W. T. Sturges, and G. Malin. "The effect of desiccation on the emission of volatile bromocarbons from two common temperate macroalgae." Biogeosciences Discussions 11, no. 7 (July 11, 2014): 10673–701. http://dx.doi.org/10.5194/bgd-11-10673-2014.
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Abstract. Exposure of intertidal macroalgae during low tide has been linked to the emission of a variety of atmospherically-important trace gases into the coastal atmosphere. In recent years, several studies have investigated the role of inorganic iodine and organoiodides as antioxidants and their emission during exposure to combat oxidative stress, yet the role of organic bromine species during desiccation is less well understood. In this study the emission of dibromomethane (CH2Br2) and bromoform (CHBr3) during exposure and desiccation of two common temperate macroalgae, Fucus vesiculosus and Ulva intestinalis, is reported. Determination of the impact exposure may have on algal physiological processes is difficult as intertidal species are adapted to desiccation and may undergo varying degrees of desiccation before their physiology is affected. For this reason we include comparisons between photosynthetic capacity (Fv / Fm) and halocarbon emissions during a desiccation time series. In addition, the role of rewetting with freshwater to simulate exposure to rain was also investigated. Our results show that an immediate flux of bromocarbons occurs upon exposure, followed by a decline in bromocarbon emissions. We suggest that this immediate bromocarbon pulse may be linked to volatilisation or emissions of existing bromocarbon stores from the algal surface rather than the production of bromocarbons as an antioxidant response.
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Solanki, Ravindra, and R. B. Patel. "Study of the Effects of Lateral Substitution (Bromo) on Mesomorphic Behaviours of Chalconyl Ester Derivatives." International Letters of Chemistry, Physics and Astronomy 64 (February 15, 2016): 135–43. http://dx.doi.org/10.56431/p-d09598.
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Novel homologous series: RO-C6H4-COO-C6H3-Br-CO-CH=CH-C6H4-OC18H3 (n) (ortho bromo to –COO-) is synthesized and studied with a view to understand and establish the relation between mesomorphic properties and the molecular structure with reference to lateral substitution of bromine (-Br) at middle phenyl ring. Chalconyl homologous series consists of thirteen between (C1 to C18) homologues. Nematogenic mesomorphism commences from C5 homologue and continue up to C18 homologue in enantiotropic manner. Transition and melting points were determined by an optical polarizing microscopy (POM) equipped with a heating stage. Textures of nematic phase are threaded or schlieren. Transition curve Cr-N/I behaved in normal manner. N-I transition curve exhibited odd-even effect up to C8 homologue. It (N-I) rises and fall with negligible deviations from its normal descending tendency which appears in case of homologues C10 to C18. Thermal stability for nematic is 142.89 °C and the mesophase lengths vary minimum 8.0 °C to maximum 26.0 °C at the C7 and C8 homologue respectively. Liquid Crystal properties of present series are evaluated and compared with structurally analogous series and relative group efficiency order derived. Thus ,present series is predominantly nematogenic with absence of Smectic property. Analytical and spectral data supported moleculars of homologues.
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Mohammed, M. J., M. S. Mahdi, A. H. Jameel, and K. M. Thalj. "THE ROLE OF LACTOBACILLUS CASEI AND LACTOBACILLUS ACIDOPHILLUS TO DECREASE THE BIOLOGICAL EFFECTS OF POTASSIUM BROMATE IN RATS." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 52, no. 1 (February 24, 2021): 70–78. http://dx.doi.org/10.36103/ijas.v52i1.1237.
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This study was conducted to investigate the ameliorative effect of lactic acid bacteria Lactobacillus casei and Lactobacillus acidophilus against Potassium bromate (25, 50) mg / kg toxicity by some physiological indicators in 35 of female rats after 21 days. The animals were divided into 7 groups within each group 5 animals weighted 140 – 155 g. The results showed a significant decrease (P<0.05) in value of Red blood cells (RBC), hemoglobin (Hb), White blood cells (WBC), Lymphocyte (LYM) and Platelets (PLT), While increasing the values of Granules (GRN). Also found that the addition of Potassium bromate Potassium bromate led to increase in cholesterol, triglyceride (TG), Low Density Lipoprotein (LDL) and blood glucose, while decreased the values of High Density Lipoprotein (HDL) for rats groups with increasing the concentration of Potassium bromate compared with control group. The addition of two types of lactic acid bacteria L. casei and L. acidophilus with Potassium bromate showed a positive effect to reducing the negative effect of Potassium bromate on blood and lipid profile parameters compared with the control group and Potassium bromate group. It is concluded that the lactic acid bacteria has protective effects and reduces the effects that Potassium bromate.
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Ghorbani, A., R. Shafiee-Nick, SA Zojaji, and MT Rajabi-Mashhadi. "Physiological effects of proinsulin-connecting peptide in human subcutaneous adipose tissue." Physiology International 104, no. 2 (June 2017): 193–205. http://dx.doi.org/10.1556/2060.104.2017.2.2.
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Recent studies suggest that proinsulin-connecting peptide (C-peptide) may exhibit characteristics of a hormone and show physiological functions in various tissues. This study was aimed to determine whether C-peptide could be involved in the regulation of lipolysis, adiponectin release, and function of mesenchymal stem cells (MSCs) in adipose tissue. Human subcutaneous adipose tissue was cultured in the presence of C-peptide. The level of lipolysis was determined by glycerol measurement in the conditioned media. Effect of C-peptide on adiponectin secretion was evaluated in differentiated adipocytes. The adipogenic and osteogenic abilities of adipose MSCs were evaluated using oil red and alizarin red staining, respectively. The tetrazolium bromide test was conducted for evaluating the effect of C-peptide on MSCs proliferation. C-peptide induced a significant decrease in basal lipolysis at concentrations of 8 and 16 nM (p < 0.05). It had no significant effects on isoproterenol-stimulated lipolysis, adiponectin secretion, and adipogenic or osteogenic differentiation of MSCs. At a concentration of 4 nM, this peptide significantly increased the proliferative capability of MSCs (p < 0.05). These results suggest that C-peptide has some physiological effects in human subcutaneous adipose tissue and contributes to the regulation of basal lipolysis and pool of MSCs.
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Kasilingam, Thavan, and C. Thangavelu. "Synergistic Effect of Cationic Surfactants in Adsorption." International Letters of Chemistry, Physics and Astronomy 83 (August 14, 2019): 31–40. http://dx.doi.org/10.56431/p-4i67c5.
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The corrosion behaviour of cationic surfactants, namely: cetyl trimethyl ammonium bromide (CTAB) and dodecyl trimethyl ammonium bromide (DTAB), have been used as corrosion inhibitors for carbon steel in well water. Protection efficiencies of the studied surfactants were depended on the hydrophobic chain length and concentration of the surfactants. The results shows that the order of protection efficiency is CTAB > DTAB. Tafel curves showed these surfactants are acting as an anodic inhibitors. Nyquist curves revealed that a protective film is formed on the metal surface. FTIR spectra suggest that the tested surfactants protective film consists of Fe2+-CTAB, Fe2+-DTAB and Zn(OH)2. Protection efficiency values and synergism parameters obtained weight loss; potentiodynamic polarization and electrochemical impedance spectroscopy are consistent.
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Hubčík, L., P. Pullmannová, S. S. Funari, F. Devínsky, and D. Uhríková. "DNA – DOPC – gemini surfactants complexes: effect of ionic strength." Acta Facultatis Pharmaceuticae Universitatis Comenianae 61, no. 2 (December 30, 2014): 26–34. http://dx.doi.org/10.2478/afpuc-2014-0013.
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AbstractThe effect of ionic strength on DNA condensation by cationic liposomes prepared as a mixture of ethane-1,2-diylbis(dodecyl-dimethylammonium bromide) (C2GS12) and dioleoylphosphatidylcholine (DOPC) was studied using fluorescence spectroscopy. The DNA condensation followed by changes in emission intensity of ethidium bromide shows a strong dependence on the ionic strength of the solution. At physiologically relevant ionic strength (0.15 mol/l NaCl), the amount of DNA condensed between lipid bilayers is approximately 40% lower compared to 0.005 mol/l NaCl. The structure of formed complexes was studied using small angle X-ray diffraction (SAXD). DNA–C2GS12–DOPC complexes form a condensed lamellar phase organisation, which is partially disrupted by the increase of ionic strength. Both the lamellar repeat distance and DNA–DNA distance show dependence on C2GS12/DOPC molar ratio, temperature and also on ionic strength. We found that the method of preparation significantly affects both the quality of organisation and the structural parameters of complexes as discussed in the paper.
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Cook, Mary R., Charles Graham, Antonio Sastre, and Mary M. Gerkovich. "Physiological and performance effects of pyridostigmine bromide in healthy volunteers: a dose-response study." Psychopharmacology 162, no. 2 (May 1, 2002): 186–92. http://dx.doi.org/10.1007/s00213-002-1074-6.
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Nampoothiri, Madhavan, Neetinkumar D. Reddy, Jessy John, Nitesh Kumar, Gopalan Kutty Nampurath, and Mallikarjuna Rao Chamallamudi. "Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells." Behavioural Neurology 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/674164.
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Insulin is a cytokine which promotes cell growth. Recently, a few published reports on insulin in different cell lines support the antiapoptotic effect of insulin. But the reports fail to explain the role of insulin in modulating glutamate-mediated neuronal cell death through excitotoxicity. Thus, we examined the neuroprotective effect of insulin on glutamate-induced toxicity on differentiated SH-SY5Y neuronal cells. Changes in cell viability were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) based assay, while apoptotic damage was detected by acridine orange/ethidium bromide and Hoechst staining. Intracellular reactive oxygen species (ROS) accumulation and morphological alterations were also measured. Treatment with glutamate induced apoptosis, elevated ROS levels and caused damage to neurons. Insulin was able to attenuate the glutamate-induced excitotoxic damage to neuronal cells.
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Chen, Qinghua, and Song Lin. "Targeting Fas-associated protein with death domain gene with chitosan-lipid nanoparticles inhibits coronary heart disease progression in mice." Materials Express 12, no. 1 (January 1, 2022): 57–63. http://dx.doi.org/10.1166/mex.2022.2119.
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Cell apoptosis or programmed cell death is a physiological phenomenon during the development of the body; however, under certain pathological conditions, insufficient, or excessive apoptosis can cause tissue damage and physiological dysfunction. This study investigated the inhibition of oxidized low-density lipoprotein (oxLDL)-induced macrophage apoptosis in mice with coronary heart disease by regulating the target Fasassociated protein with death domain (FADD) gene. We used Western blotting to measure the effect of oxLDL on mouse macrophages and observe the effects of adding simvastatin, 3-methyladenine (3-MA), self-blocking, and other drugs. The results indicated that the target FADD gene played an important role in inhibiting lactate dehydrogenase release by 13%, in the process of oxLDL-induced lipid aggregation in macrophages. The EC50 observed by dual acridine orange/ethidium bromide fluorescence-staining accounts for the proportion of FADD being reduced by 32%, indicating that the target FADD gene exhibited a significant inhibitory effect on oxLDL-induced macrophage apoptosis in coronary heart disease. This study used a novel chitosan-lipid nanoparticle as a gene carrier to significantly improve efficacy. Therefore, targeting the FADD gene using novel chitosan-lipid nanoparticles to deliver siRNA could be a potential clinical treatment in coronary heart disease.
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Kierat, Oliwia, Agata Dudek, and Lidia Adamczyk. "The Effect of the Corrosion Medium on Silane Coatings Deposited on Titanium Grade 2 and Titanium Alloy Ti13Nb13Zr." Materials 14, no. 21 (October 24, 2021): 6350. http://dx.doi.org/10.3390/ma14216350.
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The present paper focuses on the fabrication of coatings based on vinyltrimethoxysilane and the influence of various corrosion media on the coatings produced. Coatings were deposited on two substrate materials, namely, titanium Grade 2 and titanium alloy Ti13Nb13Zr, by immersion in a solution containing vinyltrimethoxysilane, anhydrous ethyl alcohol, acetic acid and distilled water. The obtained coatings were characterized in terms of surface morphology, adhesion to the substrate and corrosion resistance. As corrosion solutions, four different simulated physiological fluids, which differed in the contents of individual ions, and a 1 mol dm−3 NaBr solution were used. The chloride ions contained in the simulated physiological fluids did not lead to pitting corrosion of titanium Grade 2 and titanium alloy Ti13Nb13Zr. This investigation shows that titanium undergoes pitting corrosion in a bromide ion medium. It is demonstrated that the investigated coatings slow down corrosion processes in all corrosion media examined.
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Kim, Park, Kang, Park, Yoo, Han, Cho, Kim, and Heo. "Green Tea Seed Oil Suppressed Aβ1–42-Induced Behavioral and Cognitive Deficit via the Aβ-Related Akt Pathway." International Journal of Molecular Sciences 20, no. 8 (April 15, 2019): 1865. http://dx.doi.org/10.3390/ijms20081865.
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The aim of this study was to investigate the availability of seeds, one of the byproducts of green tea, and evaluate the physiological activity of seed oil. The ameliorating effect of green tea seed oil (GTO) was evaluated on H2O2-induced PC12 cells and amyloid beta (Aβ)1–42-induced ICR mice. GTO showed improvement of cell viability and reduced reactive oxygen species (ROS) production in H2O2-induced PC12 cells by conducting the 2′,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and 2′,7′-dichlorofluorescein diacetate (DCF-DA) analysis. Also, administration of GTO (50 and 100 mg/kg body weight) presented protective effects on behavioral and memory dysfunction by conducting Y-maze, passive avoidance, and Morris water maze tests in Aβ-induced ICR mice. GTO protected the antioxidant system by reducing malondialdehyde (MDA) levels, and by increasing superoxide dismutase (SOD) and reducing glutathione (GSH) contents. It significantly regulated the cholinergic system of acetylcholine (ACh) contents, acetylcholinesterase (AChE) activities, and AChE expression. Also, mitochondrial function was improved through the reduced production of ROS and damage of mitochondrial membrane potential (MMP) by regulating the Aβ-related c-Jun N-terminal kinase (JNK)/protein kinase B (Akt) and Akt/apoptosis pathways. This study suggested that GTO may have an ameliorating effect on cognitive dysfunction and neurotoxicity through various physiological activities.
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Wu, Xiaoliang, Lu Ju, Yafang Song, Lijun Bai, Menqian Yuan, Wanli Xu, Jing Li, Tiancheng Xu, Lixia Pei, and Jianhua Sun. "Mechanism of Colonic Slow Wave Rhythm Regulated by Electro-acupuncture Determined using Calcium-Sensitive Receptor." Acupuncture & Electro-Therapeutics Research 45, no. 2 (February 8, 2021): 47–58. http://dx.doi.org/10.3727/036012921x16112663844842.
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The calcium-sensitive receptor (CaSR) plays a role in several biological processes. However, its role in intestinal motility remains unclear. In this study, we aimed to determine the effect of electro-acupuncture (EA) at Shangjuxu (ST37) on CaSR in colonic dysplasia mice, and to explore the possible mechanism of EA regulating colonic movement. The mice were injected with nicardipine or hexamethonium bromide to induce colonic dysplasia. Intestinal transit function was assessed by twelve hours fecal granules and fecal water content percentage, while colonic slow wave was assessed by multi-channel physiological signal acquisition system, immunofluorescence and laser confocal microscopy were used to examine CaSR expression in the gastrointestinal (GI) tract of the mice. We found that the number of fecal particles, the frequency and amplitude of colonic slow wave were disrupted after nicardipine or hexamethonium bromide injection. In addition, CaSR expression in control group was mainly distributed in intestinal epithelial cells, and the morphological structure of mucosal layer was regular. Compared with control group, the structure of mucosal layer in nicardipine group and hexamethonium bromide group were all disorderly, the expression and fluorescence intensity of CaSR in nicardipine group were visible, but in hexamethonium bromide group were weakened. After EA intervention, these disorders were ameliorated, which suggested that EA at ST37 could therefore regulate colonic motility disorders via the involvement of CaSR.
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Prusaczyk, W. K., and M. N. Sawka. "Effects of pyridostigmine bromide on human thermoregulation during cold water immersion." Journal of Applied Physiology 71, no. 2 (August 1, 1991): 432–37. http://dx.doi.org/10.1152/jappl.1991.71.2.432.
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This study examined the effects of an oral 30-mg dose of pyridostigmine bromide (PYR) on thermoregulatory and physiological responses of men undergoing cold stress. Six men were immersed in cold water (20 degrees C) for up to 180 min on two occasions, once each 2 h after ingestion of PYR and 2 h after ingestion of a placebo. With PRY, erythrocyte cholinesterase inhibition was 33 +/- 12% (SD) 110 min postingestion (10 min preimmersion) and 30 +/- 7% at termination of exposure (mean 117 min). Percent cholinesterase inhibition was significantly related to lean body mass (r = -0.91, P less than 0.01). Abdominal discomfort caused termination in three of six PYR experiments but in none of the control experiments (mean exposure time 142 min). During immersion, metabolic rate, ventilatory volume, and respiratory rate increased significantly (P less than 0.05) over preimmersion levels and metabolic rate increased with duration of immersion (P less than 0.01) in both treatment but did not differ between conditions. PYR had no significant effect on rectal temperature, mean body temperature, thermal sensations, heart rate, plasma cortisol, or change in plasma volume. It was concluded that a 30-mg dose of PYR does not increase an individual's susceptibility to hypothermia during cold water immersion; however, in combination with cold stress, PYR may result in marked abdominal cramping and limit cold tolerance.
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Ono, Masaya, Yoichi Sunagawa, Saho Mochizuki, Takahiro Katagiri, Hidemichi Takai, Sonoka Iwashimizu, Kyoko Inai, et al. "Chrysanthemum morifolium Extract Ameliorates Doxorubicin-Induced Cardiotoxicity by Decreasing Apoptosis." Cancers 14, no. 3 (January 28, 2022): 683. http://dx.doi.org/10.3390/cancers14030683.
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It is well known that the anthracycline anticancer drug doxorubicin (DOX) induces cardiotoxicity. Recently, Chrysanthemum morifolium extract (CME), an extract of the purple chrysanthemum flower, has been reported to possess various physiological activities such as antioxidant and anti-inflammatory effects. However, its effect on DOX-induced cardiotoxicity is still unknown. An 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT)assay revealed that 1 mg/mL of CME reduced DOX-induced cytotoxicity in H9C2 cells but not in MDA-MB-231 cells. A TUNEL assay indicated that CME treatment improved DOX-induced apoptosis in H9C2 cells. Moreover, DOX-induced increases in the expression levels of p53, phosphorylated p53, and cleaved caspase-3,9 were significantly suppressed by CME treatment. Next, we investigated the effect of CME in vivo. The results showed that CME treatment substantially reversed the DOX-induced decrease in survival rate. Echocardiography indicated that CME treatment also reduced DOX-induced left ventricular systolic dysfunction, and a TUNEL assay showed that CME treatment also suppressed apoptosis in the mouse heart. These results reveal that CME treatment ameliorated DOX-induced cardiotoxicity by suppressing apoptosis. Further study is needed to clarify the effect of CME on DOX-induced heart failure in humans.
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Barberio, Claudia, Lucia Bianchi, Francesca Pinzauti, Tiziana Lodi, Iliana Ferrero, Mario Polsinelli, and Enrico Casalone. "Induction and characterization of morphologic mutants in a natural Saccharomyces cerevisiae strain." Canadian Journal of Microbiology 53, no. 2 (February 2007): 223–30. http://dx.doi.org/10.1139/w06-132.
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Saccharomyces cerevisiae is a good model with which to study the effects of morphologic differentiation on the ecological behaviour of fungi. In this work, 33 morphologic mutants of a natural strain of S. cerevisiae, obtained with UV mutagenesis, were selected for their streak shape and cell shape on rich medium. Two of them, showing both high sporulation proficiency and constitutive pseudohyphal growth, were analysed from a genetic and physiologic point of view. Each mutant carries a recessive monogenic mutation, and the two mutations reside in unlinked genes. Flocculation ability and responsiveness to different stimuli distinguished the two mutants. Growth at 37 °C affected the cell but not the colony morphology, suggesting that these two phenotypes are regulated differently. The effect of ethidium bromide, which affects mitochondrial DNA replication, suggested a possible “retrograde action” of mitochondria in pseudohyphal growth.
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Oršolić, Nada, Dyana Odeh, Maja Jazvinšćak Jembrek, Jelena Knežević, and Darko Kučan. "Interactions between Cisplatin and Quercetin at Physiological and Hyperthermic Conditions on Cancer Cells In Vitro and In Vivo." Molecules 25, no. 14 (July 17, 2020): 3271. http://dx.doi.org/10.3390/molecules25143271.
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Quercetin (QU), a hyperthermic sensitizer, when combined with cisplatin (CP) affects tumor growth. To determine the effects of QU and CP and their interactions, multimodal treatment in vitro and in vivo models under physiological and hyperthermic conditions was performed. In vitro, different sensitivity of T24 and UMUC human bladder cancer cells was observed after short-term exposure to QU (2 h) and CP (1 h). Effects of both compounds were investigated at low and high micromolar concentrations (1 and 50 µM, respectively) under both thermal conditions. QU acted in additive or synergistic manner in combination with CP between physiological condition and hyperthermia. As determined by 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, short-term application of QU and CP reduced cell viability. Clonal assay also indicated that combined treatment with QU and CP is lethal to bladder cancer cells in both conditions. In vivo, CP (5 or 10 mg kg−1) and QU (50 mg kg−1) acted synergistically with hyperthermia (43 °C) and inhibited tumor growth, activated immune effectors and increased mice survival. Our results demonstrate that combined treatment with CP and QU may increase death of tumor cells in physiological and hyperthermic conditions which could be clinically relevant in locoregional chemotherapy.
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Abdullah, Wael. "Effect of Halogen Doping on Optical Properties for ZnO Thin Film Prepared by Thermal Oxidation." International Letters of Chemistry, Physics and Astronomy 61 (November 3, 2015): 149–61. http://dx.doi.org/10.56431/p-hs42z8.
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Undoped and halogen-doped zinc oxide thin films are prepared by the thermal oxidation process. Zinc acetate dihydrate, ethanol, and Diethanolamine are used as precursor, solvent, and stabilizer, respectively. In the case of ZnO:Hal. dopant Ammonium chloride NH4Cl 99%, Benzene Bromide C6H5Br, or Benzene Iodide C6H5I for making dopant ZnO thin film with Cl, Br, I respectively is added to the precursor solution with an atomic percentage equal to 2-10.% hal. The transparent solution sprayed onto glass substrates, and are transformed into ZnO upon annealing at 500°C. XRD spectra of ZnO thin films, and optical properties of them as a function of halogen content have been investigated using U.V spectroscopy ( transmittance , refractive index, extinction coefficient and energy band gap ) for undoped and halogen-doped zinc oxide thin films.
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Liu, Bo, Meiling Xue, Jiao Zhou, Hongxia Zhang, Lili Ren, and Jianting Fan. "Hot Air Treatment Elicits Disease Resistance against Colletotrichum gloeosporioides and Improves the Quality of Papaya by Metabolomic Profiling." BioMed Research International 2022 (August 4, 2022): 1–13. http://dx.doi.org/10.1155/2022/5162845.
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Forced air heat treatment could induce defenses to protect fruit from pathogen attacks and has been applied as an alternative to methyl bromide for phytosanitary treatment before exportation. However, few studies were reported on the regulation mechanism of antifungal effect and delayed physiological disorders of papaya by heat treatment. Therefore, we aim to explore the fruit’s resistance to pathogens and the inhibition of physiological disorders by metabolomic profiling. In our study, papaya fruits were treated with 47.2°C for 30, 60, and 90 min by forced hot air treatment. The disease resistance against Colletotrichum gloeosporioides, quality parameters, and metabolites of papaya fruits were measured during 10 days of storage after heat treatment by metabolomic profiling. Papaya fruits after 30 and 60 min heat treatment had higher firmness, a delayed degreening and yellowing (lower a value) process, and a higher lightness (L) and hue angle (h) during storage. Heat treatment also delayed ripening, inhibiting the growth of C. gloeosporioides and softening of papaya. Metabolites and enzymes inhibited ROS scavenging, depressed ABA-regulated respiratory, and activated phenylpropanoid metabolism. Our study provides a broad picture of fruit resistance to pathogens and the inhibition of physiological disorders by metabolomic profiling, which is induced by heat treatment.
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Kaboré, A. F., M. Denis, and M. G. Bergeron. "Association of nitric oxide production by kidney proximal tubular cells in response to lipopolysaccharide and cytokines with cellular damage." Antimicrobial Agents and Chemotherapy 41, no. 3 (March 1997): 557–62. http://dx.doi.org/10.1128/aac.41.3.557.
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Recent findings suggest that nitric oxide (NO) is an important biologic mediator which exerts a wide variety of effects on numerous physiological and pathophysiological processes. L-Arginine is oxidized to L-citrulline with concomitant NO production; as a result, nitrate and nitrite accumulates. This study was conducted to determine the potential NO production by proximal tubular cells (PTC) in response to bacterial lipopolysac-charides (LPS) and cytokines and to evaluate the cytotoxic effect associated with NO release. After a 7-day stimulation with LPS (100 micrograms/ml), interleukin-1 beta (IL-1 beta) (10 ng/ml), and tumor necrosis factor alpha (TNF-alpha) (10 ng/ml), the nitrate and nitrite levels were determined by a spectrophotometric method based on the Griess reaction. Moreover, alpha-methylglucopyranoside phosphate and lactate dehydrogenase release and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay served as indicators of sodium-dependent hexose transport integrity and cell death, respectively. IL-1 beta and TNF-alpha used alone or together or combined with LPS led to a significant generation of NO by PTC. Our results also demonstrate that NO induced by LPS and cytokines could inhibit sodium-dependent transport and could induce PTC damage.
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Hargreaves, K. M., C. M. Flores, R. A. Dionne, and G. P. Mueller. "The role of pituitary beta-endorphin in mediating corticotropin-releasing factor-induced antinociception." American Journal of Physiology-Endocrinology and Metabolism 258, no. 2 (February 1, 1990): E235—E242. http://dx.doi.org/10.1152/ajpendo.1990.258.2.e235.
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The hypothesis that blood-borne beta-endorphin modulates nociception was examined with corticotropin-releasing factor (CRF) as a potent and selective agent to stimulate its release from the pituitary gland. Intravenously administered CRF produced a dose-related antinociception in rats as determined by measuring paw-lick latencies on a 50 degrees C hot plate. A dose of 25 nmol/kg of CRF was comparable in both magnitude and duration of antinociception to a 7,500 nmol/kg (= 2.5 mg/kg) dose of morphine sulfate. The antinociceptive effect of CRF was blocked by both hypophysectomy and dexamethasone pretreatment, suggesting that it was mediated by hormone release from the anterior pituitary corticotrophs. Furthermore, the effect of CRF was antagonized by 1) naltrexone, 2) naltrexone methyl bromide, and 3) passive immunization with anti-beta-endorphin antiserum. Together, these data support the hypothesis that opiate-active, beta-endorphin, released by pituitary corticotrophs, participates in the physiological modulation of nociception in rats.
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Tian, Xiang-qin, Ke-tao Ma, Xian-wei Wang, Yang Wang, Zhi-kun Guo, and Jun-qiang Si. "Effects of the Calcium-Activated Chloride Channel Inhibitors T16Ainh-A01 and CaCCinh-A01 on Cardiac Fibroblast Function." Cellular Physiology and Biochemistry 49, no. 2 (2018): 706–16. http://dx.doi.org/10.1159/000493036.
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Background/Aims: Calcium-activated chloride channels (CaCCs) regulate numerous physiological processes including cell proliferation, migration, and extracellular matrix secretion. T16Ainh-A01 and CaCCinh-A01 are selective inhibitors of CaCCs. But it is unknown whether these two compounds have functional effects on cardiac fibroblasts (CFs). Methods: Primary CFs were obtained by enzymatic dissociation of cardiomyocytes from neonatal rat hearts. Intracellular Ca2+ ([Ca2+]i) and Cl- ([Cl-]i) were measured using the fluorescent calcium indicators (Fluo-4 AM) and N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide respectively. The expression of anoctamin-1 (ANO1) and α-smooth muscle actin (α-SMA) was detected by quantitative RT-PCR, immunofluorescence, and western blotting. A hydroxyproline assay was used to examine collagen secretion. Cell proliferation, cell cycle distribution, and cell migration were assessed by Cell Counting Kit-8, flow cytometry, and Transwell assays, respectively. Results: ANO1 was preferentially expressed on the nuclear membrane and partially within intracellular compartments around the nucleus. T16Ainh-A01 and CaCCinh-A01 displayed different inhibitory effects on [Cl-]i in CFs. T16Ainh-A01 considerably decreased [Cl-]i in the nucleus, whereas CaCCinh-A01 reduced [Cl-]i in intracellular compartments around the nucleus, and both inhibitors exhibited a minimal effect on [Ca2+]i in CFs. ANO1 and α-SMA expression levels were significantly repressed by CaCCinh-A01. T16Ainh-A01 showed a marked inhibitory effect on the mRNA levels of ANO1 and α-SMA, but had a negligible effect on ANO1 at the protein level. T16Ainh-A01 and CaCCinh-A01 led to the significant repression of cell proliferation, cell migration, and collagen secretion in CFs. Conclusion: Our findings indicate that T16Ainh-A01 and CaCCinh-A01 have the potential to inhibit the proliferation and collagen secretion of CFs and may serve as novel anti-fibrotic therapeutic drugs in the future.
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Biba, Renata, Petra Cvjetko, Mirta Tkalec, Karla Košpić, Petra Peharec Štefanić, Sandra Šikić, Ana-Marija Domijan, and Biljana Balen. "Effects of Silver Nanoparticles on Physiological and Proteomic Responses of Tobacco (Nicotiana tabacum) Seedlings Are Coating-Dependent." International Journal of Molecular Sciences 23, no. 24 (December 14, 2022): 15923. http://dx.doi.org/10.3390/ijms232415923.
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The harmful effects of silver nanoparticles (AgNPs) have been confirmed in many organisms, but the mechanism of their toxicity is not yet fully understood. In biological systems, AgNPs tend to aggregate and dissolve, so they are often stabilized by coatings that influence their physico-chemical properties. In this study, the effects of AgNPs with different coatings [polyvinylpyrrolidone (PVP) and cetyltrimethylammonium bromide (CTAB)] on oxidative stress appearance and proteome changes in tobacco (Nicotiana tabacum) seedlings have been examined. To discriminate between the nanoparticulate Ag form from the ionic one, the treatments with AgNO3, a source of Ag+ ions, were also included. Ag uptake and accumulation were found to be similarly effective upon exposure to all treatment types, although positively charged AgNP-CTAB showed less stability and a generally stronger impact on the investigated parameters in comparison with more stable and negatively charged AgNP-PVP and ionic silver (AgNO3). Both AgNP treatments induced reactive oxygen species (ROS) formation and increased the expression of proteins involved in antioxidant defense, confirming oxidative stress as an important mechanism of AgNP phytotoxicity. However, the mechanism of seedling responses differed depending on the type of AgNP used. The highest AgNP-CTAB concentration and CTAB coating resulted in increased H2O2 content and significant damage to lipids, proteins and DNA molecules, as well as a strong activation of antioxidant enzymes, especially CAT and APX. On the other hand, AgNP-PVP and AgNO3 treatments induced the nonenzymatic antioxidants by significantly increasing the proline and GSH content. Exposure to AgNP-CTAB also resulted in more noticeable changes in the expression of proteins belonging to the defense and stress response, carbohydrate and energy metabolism and storage protein categories in comparison to AgNP-PVP and AgNO3. Cysteine addition significantly reduced the effects of AgNP-PVP and AgNO3 for the majority of investigated parameters, indicating that AgNP-PVP toxicity mostly derives from released Ag+ ions. AgNP-CTAB effects, however, were not alleviated by cysteine addition, suggesting that their toxicity derives from the intrinsic properties of the nanoparticles and the coating itself.
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Hantz, Holly L., Leeanne F. Young, and Keith R. Martin. "Physiologically Attainable Concentrations of Lycopene Induce Mitochondrial Apoptosis in LNCaP Human Prostate Cancer Cells." Experimental Biology and Medicine 230, no. 3 (March 2005): 171–79. http://dx.doi.org/10.1177/153537020523000303.
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Prostate cancer is the second leading cause of cancer deaths among men in the United States. Studies show that people with diets rich in tomato-based foods have reduced risks of cancer, viz., prostate cancer. This is attributed, in part, to lycopene, the most abundant carotenoid in tomatoes. Thus, we studied the effect of lycopene at physiologically attainable concentrations on apoptosis, cellular proliferation, and necrosis in LNCaP human prostate cancer cells. Cells at 37°C and >80% confluency were treated with media alone (0.32% tetrahydrofuran vehicle) or with increasing concentrations (0.3–3.0 μM) of lycopene overnight. After washing monolayers, analyses by high-performance liquid chromatography (HPLC) showed that cellular accumulation of lycopene was 5.5 ± 0.8, 14.0 ± 3.2, and 36.7 ± 12.3 pmole/106 cells for 0.3, 1.0, and 3.0 μM, respectively, and not detected in control cells. Lycopene did not alter cellular proliferation because bromodeoxyuridine (BrdU) incorporation and cell numbers were identical among groups. However, results of a 3[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay showed that mitochondrial function decreased 61%–83% with increasing concentrations of lycopene (P < 0.001). Cytotoxicity and necrosis did not contribute to this effect because lactate dehydrogenase (LDH) release (1.5%–1.8%) and trypan blue exclusion (89%–93%) were similar. Subsequently, we demonstrated that increasing concentrations of lycopene significantly (P < 0.05) reduced mitochondrial transmembrane potential, induced the release of mitochondrial cytochrome c, and increased annexin V binding, confirming induction of apoptosis. Thus, lycopene at physiologically relevant concentrations did not affect cellular proliferation or promote necrosis but clearly altered mitochondrial function and induced apoptosis in LNCaP human prostate cancer cells.
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Leiva-Salcedo, Elias, Claudio Coddou, Felipe E. Rodríguez, Antonello Penna, Ximena Lopez, Tanya Neira, Ricardo Fernández, et al. "Lipopolysaccharide Inhibits the Channel Activity of the P2X7 Receptor." Mediators of Inflammation 2011 (2011): 1–12. http://dx.doi.org/10.1155/2011/152625.
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The purinergic P2X7 receptor (P2X7R) plays an important role during the immune response, participating in several events such as cytokine release, apoptosis, and necrosis. The bacterial endotoxin lipopolysaccharide (LPS) is one of the strongest stimuli of the immune response, and it has been shown that P2X7R activation can modulate LPS-induced responses. Moreover, a C-terminal binding site for LPS has been proposed. In order to evaluate if LPS can directly modulate the activity of the P2X7R, we tested several signaling pathways associated with P2X7R activation in HEK293 cells that do not express the TLR-4 receptor. We found that LPS alone was unable to induce any P2X7R-related activity, suggesting that the P2X7R is not directly activated by the endotoxin. On the other hand, preapplication of LPS inhibited ATP-induced currents, intracellular calcium increase, and ethidium bromide uptake and had no effect on ERK activation in HEK293 cells. In splenocytes-derived T-regulatory cells, in which ATP-induced apoptosis is driven by the P2X7R, LPS inhibited ATP-induced apoptosis. Altogether, these results demonstrate that LPS modulates the activity of the P2X7R and suggest that this effect could be of physiological relevance.
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Worthen, G. S., R. S. Gumbay, D. T. Tanaka, and M. M. Grunstein. "Opposing hemodynamic effects of substance P on pulmonary vasculature in rabbits." Journal of Applied Physiology 59, no. 4 (October 1, 1985): 1098–103. http://dx.doi.org/10.1152/jappl.1985.59.4.1098.
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Substance P is a peptide implicated in the control of a variety of physiological processes. Although substance P-containing neurons impinge on the pulmonary vasculature, the effects of substance P on the pulmonary circulation have not been systematically investigated. Rabbits were anesthetized with methohexital sodium and paralyzed with pancuronium bromide. Injection of substance P (0.002–0.10 microgram/kg) in the vena cava produced dose-dependent pulmonary vasoconstriction and systemic vasodilation. Pulmonary arterial pressure reached a peak within 15–20 s and declined toward base line over 10 min. Aortic pressure fell rapidly, reaching minimum at 5–10 s. At higher doses cardiac output fell transiently, resulting in a 65% fall in pulmonary vascular conductance. If repeat substance P dosages were administered 15 min apart, there was no tachyphylaxis. Pulmonary vasoconstriction was inhibited by the cyclooxygenase blocker meclofenamate (10 mg/kg) and the thromboxane synthase inhibitor Dazmegrel (UK-38,485) (2 mg/kg). In contrast, vasoconstriction was enhanced by atropine (2 mg/kg). In Dazmegrel-treated animals in whom pulmonary vasoconstriction was established by epinephrine infusion, low doses of substance P produced vasodilation. Our findings indicate that substance P produces pulmonary vasoconstriction via prostaglandin (particularly thromboxane) generation and pulmonary vasodilation via activation of cholinergic pathways.
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Pfanzagl, Beatrix, Victor F. Zevallos, Detlef Schuppan, Roswitha Pfragner, and Erika Jensen-Jarolim. "Histamine causes influx via T-type voltage-gated calcium channels in an enterochromaffin tumor cell line: potential therapeutic target in adverse food reactions." American Journal of Physiology-Gastrointestinal and Liver Physiology 316, no. 2 (February 1, 2019): G291—G303. http://dx.doi.org/10.1152/ajpgi.00261.2018.
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The P-STS human ileal neuroendocrine tumor cells, as a model for gut enterochromaffin cells, are strongly and synergistically activated by histamine plus acetylcholine (ACh), presumably via histamine 4 receptors, and weakly activated by histamine alone. Sensing these signals, enterochromaffin cells could participate in intestinal intolerance or allergic reactions to food constituents associated with elevated histamine levels. In this study we aimed to analyze the underlying molecular mechanisms. Inhibition by mepyramine and mibefradil indicated that histamine alone caused a rise in intracellular calcium concentration ([Ca2+]i) via histamine 1 receptors involving T-type voltage-gated calcium channels (VGCCs). Sensitivity to histamine was enhanced by pretreatment with the inflammatory cytokine tumor necrosis factor-α (TNF-α). In accordance with the relief it offers some inflammatory bowel disease patients, otilonium bromide, a gut-impermeable inhibitor of T-type (and L-type) VGCCs and muscarinic ACh receptors, efficiently inhibited the [Ca2+]i responses induced by histamine plus ACh or by histamine alone in P-STS cells. It will take clinical studies to show whether otilonium bromide has promise for the treatment of adverse food reactions. The cells did not react to the nutrient constituents glutamate, capsaicin, cinnamaldehyde, or amylase-trypsin inhibitors and the transient receptor potential channel vanilloid 4 agonist GSK-1016790A. The bacterial product butyrate evoked a rise in [Ca2+]i only when added together with ACh. Lipopolysaccharide had no effect on [Ca2+]i despite the presence of Toll-like receptor 4 protein. Our results indicate that inflammatory conditions with elevated levels of TNF-α might enhance histamine-induced serotonin release from intestinal neuroendocrine cells. NEW & NOTEWORTHY We show that histamine synergistically enhances the intracellular calcium response to the physiological agonist acetylcholine in human ileal enterochromaffin tumor cells. This synergistic activation and cell activation by histamine alone largely depend on T-type voltage-gated calcium channels and are inhibited by the antispasmodic otilonium bromide. The cells showed no response to wheat amylase-trypsin inhibitors, suggesting that enterochromaffin cells are not directly involved in nongluten wheat sensitivity.
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Viana, Altevir Rossato, Angelita Bottega, Marissa Bolson Serafin, Bruno Salles, Rosmari Horner, Alexandre Krause, Luciana Maria Fontanari Krause, and Sergio Roberto Mortari. "In vitro biological activity of liposomal-containing antimony trioxide." Research, Society and Development 10, no. 11 (September 5, 2021): e391101119755. http://dx.doi.org/10.33448/rsd-v10i11.19755.
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Antimonials are used as chemotherapy for leishmaniasis, but have limited results due to their toxicity and broad resistance already acquired by the parasites. Nanotechnology offers an alternative to reduce these effects through the use of biocompatible nanocarriers, which can be vectorized to the target site. In addition, the redirection of molecules, already developed for the treatment of other pathologies, has the advantage of being already approved for therapy by regulatory agencies. The present study addresses the production of liposomal vesicles containing antimony trioxide (LC Sb2O3), as well as the evaluation of activity against tumor and bacterial cells. We produce liposomes in order of nanometric size, polydispersity index (PDI <0.3), pH value close to physiological (7.2), and zeta potential (anionic). Cytotoxicity was evaluated in 24 and 72 hours, in the HepG2, T98G, and U87MG tumor cell lines, by the method (3-4.5 dimethylthiazole-2.5 diphenyltetrazolium bromide) (MTT). The minimum inhibitory concentration (MIC) was tested on three bacterial strains (American Type Culture Collection – ATCC-Escherichia coli ATCC 35218, Staphylococcus aureus ATCC 29213 and Enterococcus faecalis ATCC 29212) and mandatory (Staphylococcus aureus and Klebsiella pneumoniae). The liposomes were more cytotoxic than Sb2O3 in the free form, for all tested cell lines. This effect was stronger after 72 hours incubation. Antimony trioxide in both free and liposomal forms showed low antibacterial activity. Based on our results, we suggest that liposomes containing antimony trioxide have the potential for the repositioning of drugs addressing anticancer therapy.
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Abdelrahman, Mutassim M., Ibrahim A. Alhidary, Riyadh S. Aljumaah, and Bernard Faye. "Blood Trace Element Status in Camels: A Review." Animals 12, no. 16 (August 18, 2022): 2116. http://dx.doi.org/10.3390/ani12162116.
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Trace minerals play an important role in animal health and productivity. They are involved also in many physiological activities, and their deficiency causes a variety of pathological problems and metabolic defects, reducing consequently the animal productivity. The demand for animal products in semi-arid areas is rapidly increasing, and the supply is still below the required level, partially due to low animal productivity. Camels (Camelus dromedarius and Camelus bactrianus) are considered one of the main sources of healthy, high-quality meat and milk for human consumption within most of the countries in the semi-arid regions. Despite their efficient adaptation to their environment, camels can suffer from the growth retardation of newborns, low feed efficiency, anemia, poor fertility, poor reproduction and many other metabolic disorders. It is well known that trace mineral deficiencies and trace mineral toxicities can influence camels’ production and reproductive efficiency, as well as many aspects of their growth and metabolism. Evaluating the trace minerals status of camels and their variability is an obvious step toward improving camels’ productivity and health. Thus, the present article reviews the data regarding the status of trace minerals (copper, zinc, iron, selenium, manganese, cobalt, iodine, fluorine, molybdenum, sulfur, bromide and nickel) in camel blood and their physiological variability, with a focus on their deficiency and toxicity effects.
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Ueda, Hirotaka, Mayu Suga, Takakazu Yagi, Ikue Kusumoto-Yoshida, Hideki Kashiwadani, Tomoyuki Kuwaki, and Shouichi Miyawaki. "Vagal afferent activation induces salivation and swallowing-like events in anesthetized rats." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 311, no. 5 (November 1, 2016): R964—R970. http://dx.doi.org/10.1152/ajpregu.00292.2016.
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The aim of this study was to clarify the effect of vagal afferent activation on salivation and swallowing-like events. Salivation is part of a reflex induced by stimulation of the oral area during feeding or chewing. Recently, we reported that nausea induced by gastroesophageal reflux (GER) activation produced salivation and swallowing in humans. Here, we investigated the ability of visceral sensation to enhance salivation and swallowing in rodents in order to inform the mechanism of GER-mediated stomatognathic activation. First, we administered LiCl to anesthetized male rats to induce nausea. LiCl significantly increased salivation and increased the activity of the vagal afferent nerve. Next, we simultaneously recorded salivation and swallowing using an electrode attached to the mylohyoid muscle during vagal afferent stimulation in a physiological range of frequencies. Vagal afferent stimulation significantly increased salivation and swallowing-like events in a frequency-dependent manner. A muscle relaxant, vecuronium bromide, diminished the swallowing-like response but did not affect salivation. These results indicate that visceral sensation induces salivation and swallowing-like events in anesthetized rodents through vagal afferent activation.
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Hunnicutt, Clinton J., Andrew W. MacRae, and Vance M. Whitaker. "Response of Four Strawberry Cultivars to Clopyralid Applied during Fruiting Stage." HortTechnology 23, no. 3 (June 2013): 301–5. http://dx.doi.org/10.21273/horttech.23.3.301.
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With the reduction in the availability of methyl bromide as a soil fumigant for Florida strawberry (Fragaria ×ananassa) culture, annual broadleaf weeds are expected to become increasingly troublesome to control. Recent studies show that along with the new fumigant systems, separate but complementary herbicide applications throughout the growing season will also be a necessity for acceptable weed control. The purpose of the study reported herein was to evaluate the impacts of multiple rates of the herbicide clopyralid on the growth and fruit production of four annual strawberry cultivars. Two greenhouse trials were conducted, evaluating the application of varying rates of clopyralid as a directed spray to well-established, mature plants of ‘Strawberry Festival’, ‘Florida Radiance’, ‘Treasure’, and Winterstar™ ‘FL 05–107’. Leaf production, leaf malformation, and marketable yield were evaluated to determine negative effects because of the physiological herbicidal effects, phytotoxic herbicidal effects, or both of clopyralid. Results from these studies showed that when clopyralid was applied at the maximum labeled rate of 3 oz/acre, less than 12% leaf malformation was observed among all cultivars, and marketable yield exhibited a linear increase as the rate of clopyralid increased, possibly due to a reduction in canopy coverage leading to more effective pollination.
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Thirunarayanan, Ganesamoorthy. "IR & NMR Spectral Studies of some 7-Substituted 9H-Fluorenacyl Bromides: Assessment of Substituent Effects." International Letters of Chemistry, Physics and Astronomy 14 (May 19, 2013): 152–61. http://dx.doi.org/10.56431/p-99j52n.
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A series of some 2-bromo-1-(2-substituted 9H-fluorene-7-yl)ethanones have been prepared. The purities of these ethanones have been checked by their physical constants and spectroscopic data. The spectral group frequencies of these enones have been assigned and correlated with Hammett substituent constants, F and R parameters. From the results of statistical analyses, the effects of substituent have been discussed.
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ASMUS, GUILHERME L., and LUIZ CARLOS C. B. FERRAZ. "Effect of population densities of Heterodera glycines race 3 on leaf area, photosynthesis and yield of soybean." Fitopatologia Brasileira 27, no. 3 (June 2002): 273–78. http://dx.doi.org/10.1590/s0100-41582002000300006.
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The effect of Heterodera glycines on photosynthesis, leaf area and yield of soybean (Glycine max) was studied in two experiments carried out under greenhouse condition. Soybean seeds were sown in 1.5 l (Experiment 1) or 5.0 l (Experiment 2) clay pots filled with a mixture of field soil + sand (1:1) sterilized with methyl bromide. Eight days after sowing, seedlings were thinned to one per pot, and one day later inoculated with 0; 1.200; 3.600; 10.800; 32.400 or 97.200 J2 juveniles of H. glycines. Experiment 1 was carried out during the first 45 days of the inoculation while Experiment 2 was conducted during the whole cycle of the crop. Measurements of photosynthetic rate, stomatic conductance, chlorophyll fluorescence, leaf color, leaf area, and chlorophyll leaf content were taken at ten-day intervals throughout the experiments. Data on fresh root weight, top dry weight, grain yield, number of eggs/gram of roots, and nematode reproduction factor were obtained at the end of the trials. Each treatment was replicated ten times. There was a marked reduction in both photosynthetic rate and chlorophyll content, as well as an evident yellowing of the leaves of the infected plants. Even at the lowest Pi, the effects of H. glycines on the top dry weight or grain yield were quite severe. Despite the parasitism, soybean yield was highly correlated with the integrated leaf area and, accordingly, the use of this parameter was suggested for the design of potential damage prediction models that include physiological aspects of nematode-diseased plants.
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Nio, D. A., R. N. Moylan, and J. K. Roche. "Modulation of T lymphocyte function by neuropeptides. Evidence for their role as local immunoregulatory elements." Journal of Immunology 150, no. 12 (June 15, 1993): 5281–88. http://dx.doi.org/10.4049/jimmunol.150.12.5281.
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Abstract In mucosa-bearing organs with inherent lymphoid populations, classical modes for control of the immune response may be augmented by products of extrinsic sensory afferent nerve endings which arborize through the lamina propria compartment containing large numbers of T and B lymphocytes. Therefore, we sought to determine the role of neuropeptides (substance P, vasoactive intestinal peptide, and somatostatin) in immune response regulation by using a homogeneous line of T lymphocytes (AO40.1 hybrid), whose activation is driven by a specific Ag (OVA) and where the end point (IL-2 release) could not be contributed to by accessory or other cells. IL-2 was quantitated by the rate of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) metabolism with the use of a murine CD4+ IL-2-dependent T lymphocyte line, and dose-response effects of each neuropeptide were examined over a broad concentration range (10(-14)-10(-6) M) encompassing that regarded as physiologic. Vasoactive intestinal peptide stimulated IL-2 release at low concentrations with a marked effect at 10(-14) M that gradually returned to control levels by 10(-7) M. Somatostatin was associated with a substantial augmentation of AO40.1 T lymphocyte IL-2 release at 10(-10) to 10(-8) M concentrations, whereas substance P demonstrated a stimulatory effect only at high concentrations (10(-9) to 10(-6) M). Concomitant [3H]thymidine uptake studies suggested that changes in cell proliferation or viability did not account for neuropeptide-induced effects in our system. With several exceptions, similar results were found with mitogen (Con A)-stimulated AO40.1 cells and human colonic lamina propria mononuclear cells. It was concluded that the three study neuropeptides, over a broad range of concentrations, have profound stimulatory (and occasionally inhibitory) effects upon the function of a cloned T lymphocyte hybrid cell responding to specific Ag and that these events may reflect those of Ag-driven mucosal T lymphocytes exposed to neuropeptides in vivo.
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Gopi, Indra Kumar, and Suresh I. S. Rattan. "Biphasic Dose–Response and Hormetic Effects of Stress Hormone Hydrocortisone on Telomerase-Immortalized Human Bone Marrow Stem Cells In Vitro." Dose-Response 17, no. 4 (October 1, 2019): 155932581988981. http://dx.doi.org/10.1177/1559325819889819.
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Although high levels of stress hormones are associated with well-known negative health outcomes, their low levels can have health-promoting effects by virtue of the phenomenon of mild stress-induced hormesis. We have studied the effects of a wide range (between 100 nmol/L and 150 μmol/L) of hydrocortisone (HC) on human bone marrow stem cells in vitro. Telomerase-immortalized human mesenchymal stem cells (hTERT-MSCs) were exposed to various doses of HC for different durations (1-6 days) and analyzed for survival and metabolic activity by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, for cell migratory ability by a wound-healing assay and for osteoblastic and adipogenic differentiation abilities in vitro. Our findings indicate that hTERT-MSCs exposed to HC resulted in a biphasic hormetic dose–response in some measures but not all. Although the mitochondrial and metabolic MTT activity assay clearly showed low-level stimulatory (between 0.1 and 1 µmol/L) and high-level inhibitory effects (from about 10 µmol/L onward), the cytostatic and differentiation-inducing effects were mostly linear at concentrations between 1 and 100 µmol/L. Further long-term studies will elucidate whether chronic or intermittent exposure of human cells to stress hormones has physiologically beneficial hormetic effects.
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Safaeian, Leila, Golnaz Vaseghi, Hedieh Jabari, and Nasim Dana. "Evolocumab, a proprotein convertase subtilisin/kexin type 9 inhibitor, promotes angiogenesis in vitro." Canadian Journal of Physiology and Pharmacology 97, no. 5 (May 2019): 352–58. http://dx.doi.org/10.1139/cjpp-2018-0542.
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The proprotein convertases family is involved in several physiological processes such as cell growth, migration, and angiogenesis, and also in different pathological conditions. Evolocumab, an inhibitor of proprotein convertase subtilisin/kexin type 9 (PCSK9), has recently been approved for treatment of hypercholesterolemia. This study aimed to investigate the effect of evolocumab on angiogenesis in human umbilical vein endothelial cells (HUVECs). Cell proliferation and migration were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell methods. In vitro angiogenesis was assessed by tube formation assay. Vascular endothelial growth factor (VEGF) secretion by HUVECs was also determined using an enzyme-linked immunosorbent assay kit. Evolocumab significantly increased HUVECs viability at 100 μg/mL. Significant enhancement in cell migration, and mean tubules length and size was observed at the concentrations of 10 and 100 μg/mL and also in mean number of junctions at the concentration of 100 μg/mL. Administration of evolocumab at the concentration of 10 μg/mL increased VEGF release into supernatants of HUVECs. Findings of this investigation provided in vitro evidence for pro-angiogenic activity of evolocumab through promoting cell proliferation, migration, tubulogenesis, and VEGF secretion in HUVECs.
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Skibinski, Grzegorz, J. Stuart Elborn, and Madeleine Ennis. "Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor." American Journal of Physiology-Lung Cellular and Molecular Physiology 293, no. 1 (July 2007): L69—L76. http://dx.doi.org/10.1152/ajplung.00299.2006.
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Proliferation of bronchial epithelial cells is an important biological process in physiological conditions and various lung diseases. The objective of this study was to determine how bronchial fibroblasts influence bronchial epithelial cell proliferation. The proliferative activity in cocultures was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and direct cells counts. Concentration of cytokines was measured in cell culture supernatants by means of ELISA. In primary cell cocultures, fibroblasts or fibroblast-conditioned medium enhanced 1.85-fold the proliferation of primary bronchial epithelial cells ( P < 0.02) compared with bronchial epithelial cells cultured alone. The proliferative activity in cocultures and in fibroblast-conditioned medium was reduced by neutralizing antibody to hepatocyte growth factor (HGF) and HGF receptor c-met. Neutralizing antibodies to FGF-7 and IGF-1 had no effect. Treatment of fibroblast-epithelial cocultures with anti-IL-6 and anti-TNF-α neutralizing antibodies and with indomethacin decreased production of HGF. These results indicate that cytokines and PGE2may indirectly mediate epithelial cell proliferation via the regulation of HGF in bronchial stromal cells and that HGF plays a crucial role in proinflammatory cytokine-induced proliferation in the experimental system studied.
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Rizzo, Maria Teresa, Elisabeth Nguyen, Marlene Aldo-Benson, and Gerard Lambeau. "Secreted phospholipase A2 induces vascular endothelial cell migration." Blood 96, no. 12 (December 1, 2000): 3809–15. http://dx.doi.org/10.1182/blood.v96.12.3809.
Full textAbstract:
Abstract Secreted phospholipase A2 (sPLA2) regulates a variety of cellular functions. The present investigation was undertaken to elucidate the potential role of sPLA2 in endothelial cell (EC) migration. Bovine aortic endothelial cells (BAECs) exposed to sPLA2 placed in the lower compartment of a modified Boyden chamber displayed increased migration compared to cells exposed to vehicle. The effect of sPLA2 on EC migration was time and dose dependent. Migration of BAECs was observed at 30 minutes, increased over 1 to 2 hours, and declined thereafter. At 2 hours of stimulation, sPLA2 (0.01-2 μmol/L) induced 1.2- to 3-fold increased cell migration compared with media alone. Among the different sPLA2s tested, bee venom, Naja naja, and porcine and human pancreatic PLA2s all evoked a migratory response in ECs. Moreover, human synovial fluid, obtained from patients with arthritis and containing sPLA2 activity, induced EC migration. Migration of ECs was significantly reduced after exposure to a catalytic site mutant of pancreatic sPLA2with decreased lipolytic activity as compared to wild-type sPLA2. Similarly, pretreatment of human synovial fluid withp-bromophenacyl bromide, an irreversible inhibitor of sPLA2, markedly decreased the ability of human synovial fluid to stimulate EC migration. Moreover, migration of ECs was stimulated on exposure to hydrolytic products of sPLA2activity including arachidonic acid, lysophosphatidic acid, and lysophosphatidylcholine. These findings suggest that sPLA2plays a physiologic role in induction of EC migration. Moreover, the effects of sPLA2 on EC migration are mediated, at least in part, by its catalytic activity.
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Rizzo, Maria Teresa, Elisabeth Nguyen, Marlene Aldo-Benson, and Gerard Lambeau. "Secreted phospholipase A2 induces vascular endothelial cell migration." Blood 96, no. 12 (December 1, 2000): 3809–15. http://dx.doi.org/10.1182/blood.v96.12.3809.h8003809_3809_3815.
Full textAbstract:
Secreted phospholipase A2 (sPLA2) regulates a variety of cellular functions. The present investigation was undertaken to elucidate the potential role of sPLA2 in endothelial cell (EC) migration. Bovine aortic endothelial cells (BAECs) exposed to sPLA2 placed in the lower compartment of a modified Boyden chamber displayed increased migration compared to cells exposed to vehicle. The effect of sPLA2 on EC migration was time and dose dependent. Migration of BAECs was observed at 30 minutes, increased over 1 to 2 hours, and declined thereafter. At 2 hours of stimulation, sPLA2 (0.01-2 μmol/L) induced 1.2- to 3-fold increased cell migration compared with media alone. Among the different sPLA2s tested, bee venom, Naja naja, and porcine and human pancreatic PLA2s all evoked a migratory response in ECs. Moreover, human synovial fluid, obtained from patients with arthritis and containing sPLA2 activity, induced EC migration. Migration of ECs was significantly reduced after exposure to a catalytic site mutant of pancreatic sPLA2with decreased lipolytic activity as compared to wild-type sPLA2. Similarly, pretreatment of human synovial fluid withp-bromophenacyl bromide, an irreversible inhibitor of sPLA2, markedly decreased the ability of human synovial fluid to stimulate EC migration. Moreover, migration of ECs was stimulated on exposure to hydrolytic products of sPLA2activity including arachidonic acid, lysophosphatidic acid, and lysophosphatidylcholine. These findings suggest that sPLA2plays a physiologic role in induction of EC migration. Moreover, the effects of sPLA2 on EC migration are mediated, at least in part, by its catalytic activity.
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Guida, Maurizio, Alessia Ligresti, Daniele De Filippis, Alessandra D'Amico, Stefania Petrosino, Mariateresa Cipriano, Giuseppe Bifulco, et al. "The Levels of the Endocannabinoid Receptor CB2 and Its Ligand 2-Arachidonoylglycerol Are Elevated in Endometrial Carcinoma." Endocrinology 151, no. 3 (March 1, 2010): 921–28. http://dx.doi.org/10.1210/en.2009-0883.
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The endocannabinoid system plays protective roles against the growth and the spreading of several types of carcinomas. Because estrogens regulate this system both in physiological states and cancer, in this paper we evaluated its involvement in endometrial carcinoma, a well-known estrogen-dependant tumor. To test whether the endocannabinoid system is expressed in endometrial cancer, tissue samples were collected both from 18 patients undergoing surgical treatment for endometrial adenocarcinoma and 16 healthy age-matched controls, and treated for Western blot and immunohistochemical analysis. Moreover, tissues were dounce homogenized and submitted to endocannabinoid measurement by liquid chromatography-mass spectrometry. To evaluate the physiological role of the endocannabinoid system, a human endometrial cancer cell-line (AN3CA) was used and transiently transfected with a plasmid containing the cDNA for the endocannabinoid receptor CB2. Cells were incubated for 48 h with an agonist (JWH133) (10 μm) or antagonist (SR144528) (1 μm) of CB2 24 h after transfection, and cell proliferation was measured by the 3-[4,5-dimethyltiazol-2yl]-2,5 diphenyltetrazolium bromide formazan assay. In human endometrial carcinoma biopsies the expression of CB2 receptor and the levels of its ligand, 2-arachidonoylglycerol increased, whereas monoacylglyerol lipase, an enzyme responsible for 2-arachidonoylglycerol degradation, was down-regulated. Immunohystochemical analysis revealed that CB2 was overexpressed only in malignant endometrial cells. CB2-overexpressing AN3CA cells showed a significant reduction in cell vitality compared with parental AN3CA cells: incubation with the selective CB2 antagonist SR144128 restored the viability of CB2-overexpressing cells to that of untransfected cells. In conclusion, the endocannabinoid system seems to play an important role in human endometrial carcinoma, and modulation of CB2 activity/expression may account for a tumor-suppressive effect.
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Moon, Jong Hyun, Jong Min Kim, Uk Lee, Jin Yong Kang, Min Ji Kim, Hyo Lim Lee, Hye Rin Jeong, et al. "Walnut Prevents Cognitive Impairment by Regulating the Synaptic and Mitochondrial Dysfunction via JNK Signaling and Apoptosis Pathway in High-Fat Diet-Induced C57BL/6 Mice." Molecules 27, no. 16 (August 20, 2022): 5316. http://dx.doi.org/10.3390/molecules27165316.
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This study was conducted to evaluate the protective effect of Juglans regia (walnut, Gimcheon 1ho cultivar, GC) on high-fat diet (HFD)-induced cognitive dysfunction in C57BL/6 mice. The main physiological compounds of GC were identified as pedunculagin/casuariin isomer, strictinin, tellimagrandin I, ellagic acid-O-pentoside, and ellagic acid were identified using UPLC Q-TOF/MS analysis. To evaluate the neuro-protective effect of GC, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′,7′-dichlorodihydrofluorecein diacetate (DCF-DA) analysis were conducted in H2O2 and high glucose-induced neuronal PC12 cells and hippocampal HT22 cells. GC presented significant cell viability and inhibition of reactive oxygen species (ROS) production. GC ameliorated behavioral and memory dysfunction through Y-maze, passive avoidance, and Morris water maze tests. In addition, GC reduced white adipose tissue (WAT), liver fat mass, and serum dyslipidemia. To assess the inhibitory effect of antioxidant system deficit, lipid peroxidation, ferric reducing antioxidant power (FRAP), and advanced glycation end products (AGEs) were conducted. Administration of GC protected the antioxidant damage against HFD-induced diabetic oxidative stress. To estimate the ameliorating effect of GC, acetylcholine (ACh) level, acetylcholinesterase (AChE) activity, and expression of AChE and choline acetyltransferase (ChAT) were conducted, and the supplements of GC suppressed the cholinergic system impairment. Furthermore, GC restored mitochondrial dysfunction by regulating the mitochondrial ROS production and mitochondrial membrane potential (MMP) levels in cerebral tissues. Finally, GC ameliorated cerebral damage by synergically regulating the protein expression of the JNK signaling and apoptosis pathway. These findings suggest that GC could provide a potential functional food source to improve diabetic cognitive deficits and neuronal impairments.
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Nyczepir, Andrew P., and Bruce W. Wood. "Foliar Nickel Application Can Increase the Incidence of Peach Tree Short Life and Consequent Peach Tree Mortality." HortScience 47, no. 2 (February 2012): 224–27. http://dx.doi.org/10.21273/hortsci.47.2.224.
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Peach tree short life (PTSL) is associated with the presence of ring nematode, Mesocriconema xenoplax, and poor orchard management practices. The ability of postplant nickel (Ni) foliar application to suppress M. xenoplax population density and thereby prolong survival of peach trees on a PTSL site infested with M. xenoplax was investigated from 2004 to 2011. For this study, the site was divided into plots, which received the following treatments: 1) Ni (foliar-applied); 2) methyl bromide fumigation (MBr); and 3) an untreated control. Peach trees were planted into all plots in Mar. 2005 and the foliar Ni treatment was applied three times in 2005 and 2006. Nickel did not detectably suppress M. xenoplax populations as compared with MBr fumigation. The protective effect of MBr fumigation in suppressing M. xenoplax population density persisted for 27 months after orchard establishment. Trees receiving multiple foliar Ni applications at 0.45 g·L−1 over 2 years, while exposed to M. xenoplax, exhibited greater PTSL mortality than trees growing in untreated or MBr-fumigated plots. These results suggest that foliar applications of Ni to peach trees, growing on a PTSL site, should be used with caution in commercial orchards because these treatments can deleteriously disrupt tree metabolic/physiological processes sufficient to increase the incidence of PTSL tree mortality.
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Herrera-Alonso, Alejandra E., María C. Ibarra-Alonso, Sandra C. Esparza-González, Sofía Estrada-Flores, Luis A. García-Cerda, and Antonia Martínez-Luévanos. "Biomimetic Growth of Hydroxyapatite on SiO2 Microspheres to Improve Its Biocompatibility and Gentamicin Loading Capacity." Materials 14, no. 22 (November 17, 2021): 6941. http://dx.doi.org/10.3390/ma14226941.
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The interest in multifunctional biomaterials to be implanted are also able to release drugs that reduce pain and inflammation or prevent a possible infection has increased. Bioactive materials such as silica (SiO2) containing surface silanol groups contribute to the nucleation and growth of hydroxyapatite (HAp) in a physiological environment. Regarding biocompatibility, the spherical shape of particles is the desirable one, since it does not cause mechanical damage to the cell membrane. In this work, the synthesis of SiO2 microspheres was performed by the modified Stöber method and they were used for the biomimetic growth of HAp on their surface. The effect of the type of surfactant (sodium dodecyl sulphate (SDS), cetyltrimethylammonium bromide (CTAB), and polyethylene glycol (PEG)), and heat treatment on the morphology and size of SiO2 particles was investigated. Monodisperse, spherical-shaped SiO2 microparticles with an average particle size of 179 nm, were obtained when using PEG (SiO2-PEG). The biomimetic growth of HAp was performed on this sample to improve its biocompatibility and drug-loading capacity using gentamicin as a model drug. Biomimetic growth of HAp was confirmed by FTIR-ATR, SEM-EDX and TEM techniques. SiO2-PEG/HAp sample had a better biocompatibility in vitro and gentamicin loading capacity than SiO2-PEG sample.
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Kamaev, Andrei V., Olga V. Trusova, Irina V. Makarova, and Dmitrii S. Korostovtsev. "Inhalation therapy for bronchial obstruction in children: traditional approaches and new opportunities." Pediatrics. Consilium Medicum, no. 2 (June 15, 2021): 123–28. http://dx.doi.org/10.26442/26586630.2021.2.200986.
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Acute respiratory infections are widespread in the pediatric population and represent a significant burden to the health care system and families of patients. Anatomic ans physiological features of preschool children and individual predisposition, especially atopic phenotype, determine high risk of complicated course of acute respiratory infections with bronchial obstruction syndrome. The etiological factors of recurrent AR are quite diverse from chronic infections to foreign bodies of the bronchi or genetically determined diseases, but the most common cause of recurrent AR in children remains bronchial asthma. Therapy of acute obstructive episode in real clinical practice is most often similar to therapy of bronchial asthma attack and includes effects on the main components of pathogenesis: contraction of smooth muscles, mucus hypersecretion and inflammatory edema of the bronchial wall. There has been accumulated a great practical experience of using combined preparation of fenoterol and ipratropium bromide, which currently exists also in the form of domestic medicinal product Astmasol-SOLOpharm. In addition to standard bronchodilator and mucoconstrictor therapy, hypertonic solutions, in particular Ingasalin 3%, are an important component of therapy of prolonged obstruction and relapse prevention. The possibilities of this remedy in shortening of terms of obstructive disorders and decrease of risk of antibiotic therapy are demonstrated by clinical example.
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Altin, J. G., P. Dieter, and F. L. Bygrave. "Evidence that Ca2+ fluxes and respiratory, glycogenolytic and vasoconstrictive effects induced by the action of platelet-activating factor and l-α-lysophosphatidylcholine in the perfused rat liver are mediated by products of the cyclo-oxygenase pathway." Biochemical Journal 245, no. 1 (July 1, 1987): 145–50. http://dx.doi.org/10.1042/bj2450145.
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The administration of ‘acetylglyceryl ether phosphorylcholine’ (AGEPC, also known as platelet-activating factor) and L-alpha-lysophosphatidylcholine (LPC) to rat livers perfused with media containing 1.3 mM-Ca2+ was followed by a concentration-dependent efflux of Ca2+ from the liver. Near-maximal response was observed at 100 nM-AGEPC and 50 microM-LPC, and resulted in a net efflux of approx. 130 nmol of Ca2+/g of liver. Onset of Ca2+ efflux occurred about 10 s after AGEPC and LPC administration, reached a maximum after about 50 s (the maximum rate of efflux was approx. 180 nmol/min per g) and thereafter decreased rapidly, and was sometimes followed by a much smaller influx of Ca2+. Sequential infusions of AGEPC or LPC, and phenylephrine, indicate that each of these agents mobilizes Ca2+ from the same intracellular source. The efflux of Ca2+ was not observed in the presence of indomethacin or bromophenacyl bromide, or when the liver was perfused with low-Ca2+-containing (25 microM) media. Other physiological responses, such as changes in respiration, glucose output and portal pressure, were also inhibited under these conditions. The results suggest that the Ca2+-flux changes and other responses are mediated by prostaglandins produced and released within the liver, possibly by cell types other than hepatocytes.
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Danesi, Francesca, Federico Ferioli, Maria Fiorenza Caboni, Elisa Boschetti, Mattia Di Nunzio, Vito Verardo, Veronica Valli, Annalisa Astolfi, Andrea Pession, and Alessandra Bordoni. "Phytosterol supplementation reduces metabolic activity and slows cell growth in cultured rat cardiomyocytes." British Journal of Nutrition 106, no. 4 (April 20, 2011): 540–48. http://dx.doi.org/10.1017/s0007114511000626.
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Besides being cholesterol-lowering agents, phytosterols (PS) can inhibit the growth and development of tumours. The anti-neoplastic activity is accounted for by PS incorporation into cell membranes, resulting in the interference of membrane functionality. The similarity between the PS cholesterol-lowering and anti-neoplastic effective doses deserves attention on the possible adverse effects even in non-neoplastic cells. To date, few studies have addressed the clarification of this important issue. In the present study, we supplemented primary, non-neoplastic neonatal rat cardiomyocytes with two different PS concentrations (3 or 6 μg/ml), both within the range of human plasma concentration. Cardiac cells were chosen as an experimental model since the heart has been reported as the target organ for subchronic toxicity of PS. Following supplementation, a dose-dependent incorporation of PS and a decrease in cholesterol content were clearly evidenced. PS did not induce apoptosis but caused a reduction in metabolic activity (measured as 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) conversion) and a slowing down of cell growth. The lower MTT conversion and the similar lactate dehydrogenase release could suggest that PS more efficiently target mitochondria than plasma membrane integrity. The replacement of cholesterol by PS could also have caused the observed slowing down of cell growth and the reduction in metabolic activity, which could rely on the PS increase, cholesterol decrease, or both. The present study is the first report on the effect of PS in cardiac cells, and although it is difficult to translate the obtained results to the health of heart tissue, it raises concerns about the safety of long-term exposure to physiologically relevant PS concentrations.
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Xue, Meilang, Suat Dervish, Kelly J. McKelvey, Lyn March, Fang Wang, Chris B. Little, and Christopher J. Jackson. "Activated protein C targets immune cells and rheumatoid synovial fibroblasts to prevent inflammatory arthritis in mice." Rheumatology 58, no. 10 (January 10, 2019): 1850–60. http://dx.doi.org/10.1093/rheumatology/key429.
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Abstract Objectives To investigate whether activated protein C (APC), a physiological anticoagulant can inhibit the inflammatory/invasive properties of immune cells and rheumatoid arthritis synovial fibroblasts (RASFs) in vitro and prevent inflammatory arthritis in murine antigen-induced arthritis (AIA) and CIA models. Methods RASFs isolated from synovial tissues of patients with RA, human peripheral blood mononuclear cells (PBMCs) and mouse thymus cells were treated with APC or TNF-α/IL-17 and the following assays were performed: RASF proliferation and invasion by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and cell invasion assays, respectively; cytokines and signalling molecules using ELISA or western blot; Th1 and Th17 phenotypes in human PBMCs or mouse thymus cells by flow cytometry. The in vivo effect of APC was evaluated in AIA and CIA models. Results In vitro, APC inhibited IL-1β, IL-17 and TNF-α production, IL-17-stimulated cell proliferation and invasion and p21 and nuclear factor κB activation in RASFs. In mouse thymus cells and human PBMCs, APC suppressed Th1 and Th17 phenotypes. In vivo, APC inhibited pannus formation, cartilage destruction and arthritis incidence/severity in both CIA and AIA models. In CIA, serum levels of IL-1β, IL-6, IL-17, TNF-α and soluble endothelial protein C receptor were significantly reduced by APC treatment. Blocking endothelial protein C receptor, the specific receptor for APC, abolished the early or preventative effect of APC in AIA. Conclusion APC prevents the onset and development of arthritis in CIA and AIA models via suppressing inflammation, Th1/Th17 phenotypes and RASF invasion, which is likely mediated via endothelial protein C receptor.
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Lee, Sung-Gyu, and Hyun Kang. "Evaluation of antioxidant and anti-inflammatory effects of three different Rubus coreanus Miq. by-products." Tropical Journal of Pharmaceutical Research 20, no. 11 (December 12, 2021): 2317–23. http://dx.doi.org/10.4314/tjpr.v20i11.13.
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Purpose: To investigate the antioxidant and anti-inflammatory properties of three different Rubus coreanus Miq. by-products in stimulated BV-2 microglial cells and explore its underlying physiological efficacy.Methods: Cell viability assessment was performed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Lipopolysaccharide (LPS) was used to activate BV-2microglia. Total polyphenol and total flavonoid contents were determined by the method of Folin-Denis. As three different Rubus coreanus Miq. by-products remaining after extraction of Rubus coreanus, High performance liquid chromatography (HPLC)) finger printing, ABST (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay, and NO (nitric oxide) inhibitory assay were performed.Results: Three different Rubus coreanus by-product extract did not exhibit any signs of cytotoxicity to BV-2 cells up to 100 μg/ml concentration (p < 0.5). The LPS-activated excessive release of NO in BV-2 cells was significantly inhibited by Rubus coreanus by-product extract (p < 0.5) at 500 μg/mL). Total polyphenol and total flavonoid contents were highest in 50 % ethanol wine processing by-product (p < 0.5 at 30, 50, 70 and 100 %, respectively). The by-product of wine processing had the lowest RC50 radical scavenging effect (16.53 μL/ml). The quercetin content of the wine processing by-product was the highest in the 70% ethanol extract at 6.26 mg/g (p < 0.5 at 30, 50, 70 and 100%, respectively).Conclusion: These results reveal that of the three other by-products, wine processing by-product has the highest antioxidant and anti-inflammatory activities. The use of these by-products has high added value for industrial production; furthermore, they are a potential treatment for various inflammatory diseases.
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Lajdova, Ingrid, Dusan Chorvat, Jr., Viera Spustova, and Alzbeta Chorvatova. "4-Aminopyridine activates calcium influx through modulation of the pore-forming purinergic receptor in human peripheral blood mononuclear cells." Canadian Journal of Physiology and Pharmacology 82, no. 1 (January 1, 2004): 50–56. http://dx.doi.org/10.1139/y03-128.
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We investigated whether 4-aminopyridine (4AP), a drug recently linked to calcium influx and apoptosis, also affected purinergic receptor channels that are known to play an important role in the activation of T lymphocytes. The application of 4AP induced a rise in [Ca2+]i that was sensitive to nickel. This action was also observed in cells in which calcium reserves were emptied using thapsigargin (Tg). However, it was not present in the absence of extracellular Ca2+, despite full internal reserves. Adenosine trisphosphate (ATP), a partial agonist and a physiological activator of purinergic receptors, also stimulated Ca2+ entry independently of the calcium release from internal compartments. The effects of 4AP and ATP were not additive when studied on the same population of cells. KN-62 inhibited an increase in calcium entry induced by 4AP, while brilliant blue G (BBG) prevented it, supporting the hypothesis that purinergic P2X7 receptors are involved in this action. Furthermore, 4AP allowed entry of ethidium bromide (314 Da) but not propidium iodide (415 Da) into the cell, also corroborating the involvement of P2X7 pores. The presented results demonstrate, for the first time in human mononuclear cells isolated from healthy volunteers, that the P2X7 channel pore is involved in the action of 4AP and intervenes in the sustained calcium entry induced in response to 4AP.Key words: calcium, human lymphocytes, 4-aminopyridine, purinergic receptors.
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Koncz, C., and J. T. Daugirdas. "Use of MQAE for measurement of intracellular [Cl-] in cultured aortic smooth muscle cells." American Journal of Physiology-Heart and Circulatory Physiology 267, no. 6 (December 1, 1994): H2114—H2123. http://dx.doi.org/10.1152/ajpheart.1994.267.6.h2114.
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A novel fluorescent indicator, N-[ethoxycarbonylmethyl]-6-methoxy-quinolinium bromide (MQAE), was used to measure intracellular chloride concentration ([Cl-]i) in primary cultures of rat aortic smooth muscle cells (VSMC). The hydrolytic and fluorescent properties of the dye were characterized. The intracellular Stern-Volmer constant was calculated to be 25 M-1. Cl- efflux curves were characteristic of saturation-type kinetics, with an apparent Michaelis-Menten constant value of 11 +/- 4.8 (SD) mM, a maximum velocity of 0.038 +/- 0.021 mM/s, and a half time (t1/2) of 9.0 +/- 3.7 min. The average efflux rate in the first 10 min (0.023 +/- 0.004 mM/s) was reduced in the presence of either 130 microM 4,4'-diisothiocyanato-dihydrostilbene-2,2'-disulfonic acid (H2DIDS) (0.014 +/- 0.006, P = 0.02) or 40 microM furosemide (0.017 +/- 0.004, P = 0.04). Restoration of physiological extracellular chloride concentration ([Cl-]o) after zero Cl- resulted in net Cl- influx with a t1/2 of 3.6 +/- 1.0 min. The initial Cl- influx rate was reduced after exposure to furosemide, from 0.069 +/- 0.006 to 0.046 +/- 0.008 mM/s, P < 0.002, and was reduced after exposure to H2DIDS from 0.102 +/- 0.013 to 0.033 +/- 0.003 mM/s, P < 0.001. Furosemide reduced the steady-state [Cl-]i from 31.6 +/- 3.2 to 26.1 +/- 2.4 mM, P < 0.01, whereas H2DIDS had little effect on [Cl-]i. Our results demonstrate that MQAE can be used to measure [Cl-]i in primary cultures of VSMC.