Academic literature on the topic 'BRET'

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Journal articles on the topic "BRET"

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Kocan, Martina, Heng B. See, Ruth M. Seeber, Karin A. Eidne, and Kevin D. G. Pfleger. "Demonstration of Improvements to the Bioluminescence Resonance Energy Transfer (BRET) Technology for the Monitoring of G Protein–Coupled Receptors in Live Cells." Journal of Biomolecular Screening 13, no. 9 (September 23, 2008): 888–98. http://dx.doi.org/10.1177/1087057108324032.

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The bioluminescence resonance energy transfer (BRET) technique has become extremely popular for studying protein-protein interactions in living cells and real time. Of particular interest is the ability to monitor interactions between G protein–coupled receptors, such as the thyrotropin-releasing hormone receptor (TRHR), and proteins critical for regulating their function, such as β-arrestin. Using TRHR/β-arrestin interactions, we have demonstrated improvements to all 3 generations of BRET (BRET1, BRET2, and eBRET) by using the novel forms of luciferase, Rluc2 and Rluc8, developed by the Gambhir laboratory. Furthermore, for the 1st time it was possible to use the BRET2 system to detect ligand-induced G protein–coupled receptor/β-arrestin interactions over prolonged periods (on the scale of hours rather than seconds) with a very stable signal. As demonstrated by our Z′-factor data, these luciferases increase the sensitivity of BRET to such an extent that they substantially increase the potential applicability of this technology for effective drug discovery high-throughput screening. ( Journal of Biomolecular Screening 2008:888-898)
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Bae Kim, Sung, Rika Fujii, Arutselvan Natarajan, Tarik F. Massoud, and Ramasamy Paulmurugan. "Ligand-activated BRET9 imaging for measuring protein–protein interactions in living mice." Chemical Communications 56, no. 2 (2020): 281–84. http://dx.doi.org/10.1039/c9cc07634d.

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We engineered a conceptually unique ligand-activatable BRET system (termed BRET9). This system simultaneously enhanced both the total bioluminescence spectrum and the BRET signal in the far-red region as a robust optical platform for animal imaging.
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RAMSAY, Douglas, Elaine KELLETT, Mary McVEY, Stephen REES, and Graeme MILLIGAN. "Homo- and hetero-oligomeric interactions between G-protein-coupled receptors in living cells monitored by two variants of bioluminescence resonance energy transfer (BRET): hetero-oligomers between receptor subtypes form more efficiently than between less closely related sequences." Biochemical Journal 365, no. 2 (July 15, 2002): 429–40. http://dx.doi.org/10.1042/bj20020251.

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Homo- and hetero-oligomerization of G-protein-coupled receptors (GPCRs) were examined in HEK-293 cells using two variants of bioluminescence resonance energy transfer (BRET). BRET2 (a variant of BRET) offers greatly improved separation of the emission spectra of the donor and acceptor moieties compared with traditional BRET. Previously recorded homo-oligomerization of the human δ-opioid receptor was confirmed using BRET2. Homo-oligomerization of the κ-opioid receptor was observed using both BRET techniques. Both homo- and hetero-oligomers, containing both δ- and κ-opioid receptors, were unaffected by the presence of receptor ligands. BRET detection of opioid receptor homo- and hetero-oligomers required expression of 50000–100000 copies of the receptor energy acceptor construct per cell. The effectiveness of δ—κ-opioid receptor hetero-oligomer formation was as great as for homomeric interactions. The capacity of the two opioid receptors to form oligomeric complexes with the β2-adrenoceptor was also assessed. Although such interactions were detected, at least 250000 copies per cell of the energy acceptor were required. Requirement for high levels of receptor expression was equally pronounced in attempts to measure hetero-oligomer formation between the κ-opioid receptor and the thyrotropin-releasing hormone receptor-1. These studies indicate that constitutively formed homo- and hetero-oligomers of opioid receptor subtypes can be detected in living cells containing less than 100000 copies of the receptors. However, although hetero-oligomeric interactions between certain less closely related GPCRs can be detected, they appear to be of lower affinity than homo- or hetero-oligomers containing closely related sequences. Interactions recorded between certain GPCR family members in heterologous expression systems are likely to be artefacts of extreme levels of overexpression.
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Parkes. "Reply to Bret Davis." Journal of Nietzsche Studies 46, no. 1 (2015): 82. http://dx.doi.org/10.5325/jnietstud.46.1.0082.

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Scharnhorst, Gary. "Byron and Bret Harte." Byron Journal 32, no. 1 (January 2004): 45–50. http://dx.doi.org/10.3828/bj.32.1.6.

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Scharnhorst, Gary. "Browning and Bret Harte." ANQ: A Quarterly Journal of Short Articles, Notes and Reviews 12, no. 3 (January 1999): 41–43. http://dx.doi.org/10.1080/08957699909598065.

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Feugang, J. M., R. C. Youngblood, A. Fahad, J. M. Greene, S. T. Willard, and P. L. Ryan. "73 APPLICATION OF QUANTUM DOT CONJUGATES FOR INVESTIGATING MAMMALIAN SPERMATOZOA." Reproduction, Fertility and Development 24, no. 1 (2012): 149. http://dx.doi.org/10.1071/rdv24n1ab73.

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Self-illuminating quantum dots are nanoparticles that are less than 100 nm in diameter. Their coating with the light-emitting protein Renilla luciferase forms complexes that have promising applications in in vivo imaging. These complexes can be further combined to specific tags such as antibodies or peptides for various in vitro studies. Especially in reproduction, these conjugates may contribute to a better comprehension of molecular events associated with fertilization and beyond. To this end, we evaluated the ability of mammalian spermatozoa to harmlessly incorporate nanoparticles. Motile spermatozoa of freshly collected boar and stallion semen were purified, washed in PBS/polyvinylpyrrolidone (PVP) (1 mg mL–1) and adjusted at desired concentrations according to experiments. Spermatozoa were fixed at 107 in Experiment 1 and incubated for 30 min at 37°C with 0, 1, or 5 nM quantum dots conjugated with the bioluminescence resonance energy transfer and R9 cell internalization peptide (BRET-Qdot-R9). In Experiment 2, different amounts of spermatozoa (25 × 106, 50 × 106 and 100 × 106) were incubated as in Experiment 1 with 0 or 1 nM BRET-Qdot-R9. After incubation, aliquots of BRET-Qdot-R9-loaded spermatozoa in both experiments were set aside for motility analysis using the computer-assisted sperm analyzer (only boar data shown). Remaining spermatozoa were centrifuged to eliminate the excess of BRET-Qdot-R9 and washed twice with PBS/PVP at 22°C. Resulting pellets were re-suspended with 50 μL of PBS/PVP and aliquots were mounted on slides for confocal fluorescence microscopic evaluation. Remaining cells and supernatants were mixed with 2 μg of coelenterazine and immediately imaged for bioluminescence using the IVIS-100 Imaging System. Experiments were repeated 3 times and analysed (Student's t-test; P < 0.05 for threshold of significance). Higher light emissions were detected in tubes containing both spermatozoa and BRET-Qdot-R9 as compared to the control (0 nM BRET-Qdot-R9) and last wash-derived supernatants. Low background signals of coelenterazine were observed in tubes (± sperm) without BRET-Qdot-R9. Experiment 1 revealed a dose-dependent response of BRET-Qdot-R9, whereas experiment 2 indicated a potential decrease of light emission with the higher sperm quantity. Interestingly, the presence of BRET-Qdot-R9 did not compromise sperm motility; however, the sperm/BRET-Qdot-R9 ratio appears essential to maintain comparable proportions of motile and rapid spermatozoa to the control group (86 ± 6 and 74 ± 5% for control vs 63 ± 17 and 46 ± 15%, 81 ± 8 and 68 ± 11% and 93 ± 1 and 85 ± 1% for 25 × 106, 50 × 106 and 100 × 106 sperm/1 nM ratios, respectively; P ≥ 0.05; mean ± standard error of the means). The BRET-Qdot-R9 fluorescence signal was mostly detected in the sperm head and intermediate piece with 1 nM BRET-Qdot-R9, whereas the entire spermatozoa fluoresced with 5 nM BRET-Qdot-R9. This study indicates that the incorporation of BRET-Qdot-R9 by boar and stallion spermatozoa does not impair their motility. Further investigations are needed to evaluate the proportion of cells incorporating BRET-Qdot-R9. Supported by the USDA-ARS Biophotonics Initiative #58-6402-3-0120.
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Cooray, Sadani N., Teng-Teng Chung, Khansa Mazhar, Laszlo Szidonya, and Adrian J. L. Clark. "Bioluminescence Resonance Energy Transfer Reveals the Adrenocorticotropin (ACTH)-Induced Conformational Change of the Activated ACTH Receptor Complex in Living Cells." Endocrinology 152, no. 2 (February 1, 2011): 495–502. http://dx.doi.org/10.1210/en.2010-1053.

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Abstract The melanocortin 2 receptor (MC2R) accessory protein (MRAP) is a small single-transmembrane domain protein that plays a pivotal role in the function of the MC2R. The pituitary hormone, ACTH, acts via this receptor complex to stimulate adrenal steroidogenesis. Using both coimmunoprecipitation and bioluminescence resonance energy transfer (BRET), we show that the MC2R is constitutively homodimerized in cells. Furthermore, consistent with previous data, we also show that MRAP exists as an antiparallel homodimer. ACTH enhanced the BRET signal between MC2R homodimers as well as MC2R-MRAP heterodimers. However, ACTH did not enhance the physical interaction between these dimers as determined by coimmunoprecipitation. Real-time BRET analysis of the MRAP-MC2R interaction revealed two distinct phases of the ACTH-dependent BRET increase, an initial complex series of changes occurring over the first 2 min and a later persistent increase in BRET signal. The slower ACTH-dependent phase was inhibited by the protein kinase A inhibitor KT5720, suggesting that signal transduction was a prerequisite for this later conformational change. The MRAP-MC2R BRET approach provides a unique tool with which to analyze the activation of this receptor.
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Wang, Lufei, Dong Joon Lee, Han Han, Lixing Zhao, Hiroshi Tsukamoto, Yong-IL Kim, Adele M. Musicant, et al. "Application of bioluminescence resonance energy transfer-based cell tracking approach in bone tissue engineering." Journal of Tissue Engineering 12 (January 2021): 204173142199546. http://dx.doi.org/10.1177/2041731421995465.

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Bioluminescent imaging (BLI) has emerged as a popular in vivo tracking modality in bone regeneration studies stemming from its clear advantages: non-invasive, real-time, and inexpensive. We recently adopted bioluminescence resonance energy transfer (BRET) principle to improve BLI cell tracking and generated the brightest bioluminescent signal known to date, which thus enables more sensitive real-time cell tracking at deep tissue level. In the present study, we brought BRET-based cell tracking strategy into the field of bone tissue engineering for the first time. We labeled rat mesenchymal stem cells (rMSCs) with our in-house BRET-based GpNLuc reporter and evaluated the cell tracking efficacy both in vitro and in vivo. In scaffold-free spheroid 3D culture system, using BRET-based GpNLuc labeling resulted in significantly better correlation to cell numbers than a fluorescence based approach. In scaffold-based 3D culture system, GpNLuc-rMSCs displayed robust bioluminescence signals with minimal background noise. Furthermore, a tight correlation between BLI signal and cell number highlighted the robust reliability of using BRET-based BLI. In calvarial critical sized defect model, robust signal and the consistency in cell survival evaluation collectively supported BRET-based GpNLuc labeling as a reliable approach for non-invasively tracking MSC. In summary, BRET-based GpNLuc labeling is a robust, reliable, and inexpensive real-time cell tracking method, which offers a promising direction for the technological innovation of BLI and even non-invasive tracking systems, in the field of bone tissue engineering.
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Hwang, Eugene, Jisu Song, and Jin Zhang. "Integration of Nanomaterials and Bioluminescence Resonance Energy Transfer Techniques for Sensing Biomolecules." Biosensors 9, no. 1 (March 16, 2019): 42. http://dx.doi.org/10.3390/bios9010042.

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Bioluminescence resonance energy transfer (BRET) techniques offer a high degree of sensitivity, reliability and ease of use for their application to sensing biomolecules. BRET is a distance dependent, non-radiative energy transfer, which uses a bioluminescent protein to excite an acceptor through the resonance energy transfer. A BRET sensor can quickly detect the change of a target biomolecule quantitatively without an external electromagnetic field, e.g., UV light, which normally can damage tissue. Having been developed quite recently, this technique has evolved rapidly. Here, different bioluminescent proteins have been reviewed. In addition to a multitude of bioluminescent proteins, this manuscript focuses on the recent development of BRET sensors by utilizing quantum dots. The special size-dependent properties of quantum dots have made the BRET sensing technique attractive for the real-time monitoring of the changes of target molecules and bioimaging in vivo. This review offers a look into the basis of the technique, donor/acceptor pairs, experimental applications and prospects.
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Dissertations / Theses on the topic "BRET"

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Eustace, Natalie Margaret. "Biological Realistic Education Technology (BRET)." Thesis, University of Canterbury. HIT Lab NZ, 2014. http://hdl.handle.net/10092/9242.

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The aim of this project was to develop and evaluate an interactive Augmented Reality interface for teaching children aged 8 to 15 about biological systems present in the human body. The interface was de- signed as one component of a “human body scanner” exhibit, which is to be featured at the ScienceAlive! Science Centre. In the exhibit, the interface allows visualization and interaction with the body systems while being moved across a human male mannequin named BRET. Prior research has shown that Augmented Reality, Visualization applications, and games are viable methods to teach biology to university aged users, and Augmented Reality and interactive systems have been used with children and learning biology as well. BRET went through three iteration phases, in the first phase, prototypes were evaluated by ScienceAlive! and designs and interactions were implemented, while the use of Augmented Reality through a transparent display was rejected. Iteration two included integration of the non-transparent touch display screen and observational evaluation of six children from 9 to 15 years old. This evaluation resulted in design and interaction changes. Iteration three was the last iteration where final interface and interaction modifications were made and re- search was conducted with 48 children from the ages 8 to 15. This was to determine whether learning, fun, and retention rates were higher for children who interacted with BRET versus those who watched video clips, or read text. Each child used one learning method to learn the three different body systems: skeletal, circulatory, and digestion. The results of the final evaluation showed that overall there was no significant difference in the children’s rating of fun or the amount of information they retained between the different learning methods. There was a positive significant difference between some of the expected fun scores and the actual fun scores. It was also found that learning with text was higher than the interactive condition but there was no differences between learning with video and interaction, or with text and video.
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Borghei, Golnaz. "Design of a BRET fluorescent protein." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607666.

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Young, Joshua B. "Bret & Vince Get Framed for Murder." Ohio University Honors Tutorial College / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1339258933.

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Weissenberg, Clare. "This is not an exit : reading Bret Easton Ellis." Thesis, University of Essex, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361020.

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Nystrand, Alexander. "Patrick Bateman, Violence and Consumption: Bret Easton Ellis’s American Psycho." Thesis, Södertörns högskola, Institutionen för kultur och kommunikation, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-7875.

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This essay investigates how Bret Easton Ellis portrays Patrick Bateman as a projection of American society, in order to criticize consumerism and capitalism in his novel American Psycho. By applying Marxist theory, this essay examines Bateman's consumption patterns and class-consciousness using key Marxist terms. This essay investigates the relationship between Bateman and his commodities, through the Marxist concept of value. Furthermore, this essay suggests that Bateman's consumption pattern creates his identity and that Bateman's lust for consumption has no boundaries. Bateman quenches his thirst for consumption by consuming humans of low status on the social hierarchy, by acts of violence, rape or cannibalism.
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Silva, Luciano Cabral da. "The fourfold serial killer in Bret Easton Elliss American Psycho." Universidade do Estado do Rio de Janeiro, 2015. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=8749.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico
Patrick Bateman, o protagonista narrador do romance American Psycho (1991), de Bret Easton Ellis, confunde por ser rico, bonito e educado e, ao mesmo tempo, torturador, assassino e canibal. Mas esta personalidade antagônica não o torna singular. O que o particulariza são as quatro faces que ele apresenta ao longo de sua narrativa: (1) ele consome mercadorias e humanos, (2) compete para ter reconhecimento, (3) provoca horror por suas ações, e (4) não é um narrador confiável. Sendo um yuppie (termo popular usado nos Estados Unidos na década de 1980 para denominar jovens e bem sucedidos profissionais urbanos), Bateman é materialista e hedonista. Ele está imerso em uma sociedade de consumo, fato que o impossibilita de perceber diferenças entre produtos e pessoas. Sendo um narcisista, ele se torna um competidor em busca de admiração. No entanto, Bateman também é um serial killer e suas descrições detalhadas de torturas e assassinatos horrorizam. Por fim, nós leitores duvidamos de sua narrativa ao notarmos inconsistências e ambiguidades. Zygmunt Bauman (2009) afirma que uma sociedade extremamente capitalista transforma tudo que nela existe em algo consumível. Christopher Lasch (1991) afirma que o lendário Narciso deu lugar a um novo, controverso, dependente e menos confiante. A maioria das vítimas de Bateman são membros de grupos socialmente marginalizados, como mendigos, homossexuais, imigrantes e prostitutas, o que o torna uma identidade predatória, segundo Arjun Appadurai (2006). A voz autodiegética e a narrativa incongruente do protagonista, contudo, impedem que confiemos em suas palavras. Estas são as quatro faces que pretendo apresentar deste serial killer
The autodiegetic protagonist Patrick Bateman, in Bret Easton Elliss American Psycho (1991), is a troubling character, for he is highly-educated, wealthy and handsome as well as a torturer, a killer and a cannibal. This antagonistic behavior, nonetheless, does not make him a singular character. The four sides he presents throughout the novel are singular, though: (1) he consumes humans and commodities equally; (2) he competes for recognition and admiration; (3) his acts are horrific; and (4) his narration is unreliable. As a yuppie (a popular term from the 1980s used to define young urban U.S. professionals), Bateman is materialistic and hedonistic. As he lives off the excesses of a consumer society, he is incapable of distinguishing people from products. As a self-absorbed, narcissistic protagonist, he becomes a competitor struggling to get approval from his peers. Nevertheless, Bateman is a serial killer, and his detailed descriptions of tortures and murders are horrifying. Finally, we readers cannot rely on his narrative once we notice ambiguities and divergences. Zygmunt Bauman (2009) posits that an extremely capitalist society forces people to be commodified. Christopher Lasch (1991) asseverates that the old legendary Narcissus gave birth to a new one, paradoxical, dependent and less confident. Most of Batemans victims are socially-marginalized characters, members of minority groups, such as homeless people, homosexuals, immigrants, and prostitutes. As a matter of fact, Bateman may be regarded as having a predatory identity, as defined by Arjun Appadurai (2006). However, this autodiegetic narrator, together with his inconsistent narrative, cannot be entirely trusted. These are the points I want to debate regarding this fourfold serial killer
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Helm, Kimberly Anne. "Is everything disposable? Bret Easton Ellis, abortion, and consumer culture." [Gainesville, Fla.] : University of Florida, 2004. http://purl.fcla.edu/fcla/etd/UFE0004643.

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Issafras, Hassan. "Étude de l'oligomérisation du récepteur CCR5 par la technique de BRET." Paris 7, 2002. http://www.theses.fr/2002PA077211.

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Lacaze, Annie. "Hysteroplastie selon Bret-Palmer : à propos de 43 cas d'utérus cloisonnés." Université Louis Pasteur (Strasbourg) (1971-2008), 1986. http://www.theses.fr/1986STR1M059.

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Armando, Sylvain. "Structure quaternaire des récepteurs de chimiokines CXCR4 et CCR2 et interaction avec leur effecteurs." Thesis, Montpellier 2, 2010. http://www.theses.fr/2010MON20208/document.

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Les récepteurs couplés aux protéines G (RCPG) sont la famille de récepteurs membranaires la plus représentée chez les vertébrés, et la plus grande cible thérapeutique chez l'Homme. L'évolution du paradigme initial qui énonçait une stœchiométrie récepteur : protéine G : effecteur de 1 :1 :1 sera présentée sur le modèle des récepteurs aux chimiokines CXCR4 et CCR2. Grâce à la technique de transfert d'énergie par bioluminescence (BRET), les travaux réalisés durant cette thèse montrent (1) que c'est par un couplage alternatif de CXCR4 à Gα13 au lieu de la voie classique Gαi que les cellules de cancer du sein migrent pour former des métastases, (2) que la désensibilisation de CXCR4 implique le recrutement d'une combinaison définie de protéines (GRK et arrestines) permettant l'arrêt sélectif des multiples voies engagées en réponse à l'agoniste, et (3) que le protomère CXCR4 a un rôle déterminant dans l'engagement de la protéine Gαi et le recrutement de la β-arrestine par l'hétéro-oligomère CXCR4/CCR2 lorsque CCR2 est activé. Dans cette dernière et principale étude, les résultats montrent également que le dimère CCR2 peut s' assembler au dimère CXCR4 pour former un tétramère, et que l'activation de CCR2 influence la conformation du dimère CXCR4. Les phénomènes de coopérativité et d'activation asymétrique déjà rapportés pour cet hétérodimère pourraient donc impliquer l'interaction de quatre protomères. En conclusion les travaux effectués durant cette thèse démontrent une régulation supplémentaire de l'activité des récepteurs chimiokines au niveau de leur structure quaternaire, de leur signalisation, et de l'arrêt de cette signalisation
G protein coupled receptors (GPCR) are the most represented cell surface receptors among vertebrates, and the major therapeutic target in humans. The initial paradigm stating a 1 :1 :1 stoichiometry for receptor :G protein :effector has evolved to a more complex model, as illustrated here with the example of the chemokine receptors CXCR4 and CCR2. Bioluminescence resonance energy transfer (BRET) was used to demonstrate that (1) CXCR4 is able to couple Gα13 instead of Gαi to promote breast cancer metastasis, (2) the multiple pathways engaged by stimulation of CXCR4 are selectively desensitized by the specific recruitment of a defined combination of proteins (GRKs and arrestins) and (3) the CXCR4 protomer plays a crucial role during Gαi engagement and β-arrestin recruitment by the CXCR4/CCR2 heterodimer upon CCR2 activation. In this last and main study, the results shown also demonstrate that CCR2 dimers could assemble with CX CR4 dimers into hetero-tetramers, and that CCR2 activation leads to a conformational change in the CXCR4 dimer. Former results showing cooperativity and asymmetric activation of a simple CXCR4/CCR2 heterodimer could then be applied to a tetramer. To conclude, the work done during this thesis demonstrates a more sophisticated regulation of chemokine receptors than previously suspected at 3 different levels: quaternary structure of the protomers, G protein signalling, and signalling termination
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Books on the topic "BRET"

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Scharnhorst, Gary. Bret Harte. New York: Twayne, 1992.

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Colby, Georgina. Bret Easton Ellis. New York: Palgrave Macmillan US, 2011. http://dx.doi.org/10.1057/9780230339163.

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Scharnhorst, Gary. Bret Harte: A bibliography. Lanham, Md: Scarecrow Press, 1995.

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Gary, Scharnhorst, ed. Selected letters of Bret Harte. Norman: University of Oklahoma Press, 1997.

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Nissen, Axel. Bret Harte: Prince and pauper. Jackson: University Press of Mississippi, 2000.

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1981-, Varga Péter L., ed. Az eltűnés könyvei: Bret Easton Ellis. Budapest: Palimpszeszt, 2012.

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1836-1902, Harte Bret, ed. Apo ton Bret Chart ston Papadiamantē. Athēna: Ekdoseis Domos, 1992.

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Nissen, Axel. Calendar of the letters of Bret Harte. [Oslo]: Dept. of British and American Studies, University of Oslo, 1997.

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Boynton, Henry Walcott. Bret Harte. Curzon Press, 2007.

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Brenner, Richard. Bret Favre. East End Pub, 1999.

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Book chapters on the topic "BRET"

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Ensslen, Klaus. "Harte, Bret." In Kindlers Literatur Lexikon (KLL), 1. Stuttgart: J.B. Metzler, 2020. http://dx.doi.org/10.1007/978-3-476-05728-0_5433-1.

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Annesley, James. "Bret Easton Ellis." In A Companion to Twentieth-Century United States Fiction, 514–21. Oxford, UK: Wiley-Blackwell, 2009. http://dx.doi.org/10.1002/9781444310108.ch50.

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Leypoldt, Günter. "Ellis, Bret Easton." In Kindlers Literatur Lexikon (KLL), 1. Stuttgart: J.B. Metzler, 2020. http://dx.doi.org/10.1007/978-3-476-05728-0_5235-1.

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Kalkert, Bernadette. "Bret Easton Ellis." In Kindler Kompakt Amerikanische Literatur 20. Jahrhundert, 198–200. Stuttgart: J.B. Metzler, 2015. http://dx.doi.org/10.1007/978-3-476-05528-6_44.

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Colby, Georgina. "INTRODUCTION: Underwriting the Contemporary." In Bret Easton Ellis, 1–22. New York: Palgrave Macmillan US, 2011. http://dx.doi.org/10.1057/9780230339163_1.

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Colby, Georgina. "Missing Persons: Melancholy as Symptom in Less Than Zero, The Rules of Attraction and The Informers." In Bret Easton Ellis, 23–57. New York: Palgrave Macmillan US, 2011. http://dx.doi.org/10.1057/9780230339163_2.

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Colby, Georgina. "An Inner Critique: Commodity Fetishism, Systemic Violence, and the Abstract Mutilated Subject in American Psycho." In Bret Easton Ellis, 59–94. New York: Palgrave Macmillan US, 2011. http://dx.doi.org/10.1057/9780230339163_3.

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Colby, Georgina. "Cloning the Nineties: Cultural Amnesia, Terrorism, and Contemporary Iconoclasm in Glamorama." In Bret Easton Ellis, 95–129. New York: Palgrave Macmillan US, 2011. http://dx.doi.org/10.1057/9780230339163_4.

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Colby, Georgina. "Twenty-First-Century Gothic (or post-9/11 Fatalism): Self-Parody, Reification, and the Becoming Real of Cultural and Authorial Fictions in Lunar Park." In Bret Easton Ellis, 131–63. New York: Palgrave Macmillan US, 2011. http://dx.doi.org/10.1057/9780230339163_5.

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Colby, Georgina. "CODA: The Politics of Exposure: Unsafe Lines and Narratives of Conflict in Imperial Bedrooms." In Bret Easton Ellis, 165–88. New York: Palgrave Macmillan US, 2011. http://dx.doi.org/10.1057/9780230339163_6.

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Conference papers on the topic "BRET"

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Otsuji, Tomomi, Emiko Okuda-Ashitaka, Satoshi Kojima, Hidehumi Akiyama, Seiji Ito, and Yoshihiro Ohmiya. "Application to processing system using intra-molecular BRET." In Biomedical Optics 2003, edited by Alexander P. Savitsky, Darryl J. Bornhop, Ramesh Raghavachari, and Samuel I. Achilefu. SPIE, 2003. http://dx.doi.org/10.1117/12.477879.

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Xu, ErZhuo, Donghong Qin, Jun Huang, and Jinbo Zhang. "Multi Text Classification Model Based on BRET-CNN-BiLSTM." In 2022 IEEE 5th International Conference on Big Data and Artificial Intelligence (BDAI). IEEE, 2022. http://dx.doi.org/10.1109/bdai56143.2022.9862653.

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Ivanova, Lyubov A. "Benjamin Disraeli’S Literary Heritage In Bret Harte’S Parody "Lothaw"." In International Scientific Conference «PERISHABLE AND ETERNAL: Mythologies and Social Technologies of Digital Civilization-2021». European Publisher, 2021. http://dx.doi.org/10.15405/epsbs.2021.12.03.64.

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Ivanova, Lyubov. "Romantic Aesthetics In The Parody «Miss Mix» By Bret Harte." In International Scientific and Practical Conference «MAN. SOCIETY. COMMUNICATION». European Publisher, 2021. http://dx.doi.org/10.15405/epsbs.2021.05.02.52.

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Zhan, Denghuang, Longyi Chen, Wai Hei Tse, Longyan Chen, and Jin Zhang. "Bioluminescence Resonance Energy Transfer (BRET) Based Nanostructured Biosensor for Tear Glucose Detection." In International Conference of Theoretical and Applied Nanoscience and Nanotechnology. Avestia Publishing, 2017. http://dx.doi.org/10.11159/tann17.122.

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BERTHOLD, F., C. H. JOHNSON, A. KANAUCHI, A. HEDING, M. PEUKERT, M. HENNECKE, and B. HUTTER. "DEVELOPMENT OF A SENSITIVE INSTRUMENT FOR BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET) APPLICATIONS." In Bioluminescence and Chemiluminescence - Progress and Current Applications - 12th International Symposium on Bioluminescence (BL) and Chemiluminescence (CL). WORLD SCIENTIFIC, 2002. http://dx.doi.org/10.1142/9789812776624_0042.

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Garden, Padric M., and Allison M. Dennis. "Oriented conjugation of luciferase to infrared quantum dots enables BRET-based enzyme sensing." In Colloidal Nanoparticles for Biomedical Applications XVII, edited by Marek Osiński and Antonios G. Kanaras. SPIE, 2022. http://dx.doi.org/10.1117/12.2608887.

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Michelini, Elisa, Mara Mirasoli, Matti Karp, Marko Virta, and Aldo Roda. "Development of a bioluminescence resonance energy transfer (BRET) for monitoring estrogen receptor alpha activation." In Biomedical Optics 2004, edited by Alexander P. Savitsky, Lubov Y. Brovko, Darryl J. Bornhop, Ramesh Raghavachari, and Samuel I. Achilefu. SPIE, 2004. http://dx.doi.org/10.1117/12.529246.

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Robers, Matthew B., Thomas Machleidt, Thomas Kirkland, Carolyn Woodroofe, Frank Fan, Mei Cong, and Keith Wood. "Abstract 4272: NanoLucTMas an improved BRET donor to monitor EGFR interactions within living cells." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-4272.

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MICHELINI, E., M. MAGLIULO, M. BARALDINI, and A. RODA. "NOVEL BRET-BASED BIOSENSORS WITH A NEW BIOLUMINESCENT DONOR, GAUSSIA LUCIFERASE, FOR ESTROGEN RECEPTOR LIGANDS." In Chemistry, Biology and Applications. WORLD SCIENTIFIC, 2007. http://dx.doi.org/10.1142/9789812770196_0030.

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Reports on the topic "BRET"

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Shellenbarger, Zane, and Joseph H. Abeles. Bistable Reflective Etalon (BRET). Fort Belvoir, VA: Defense Technical Information Center, September 2003. http://dx.doi.org/10.21236/ada418753.

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Yang, Kaiyue. Brew Culture. Ames (Iowa): Iowa State University, January 2019. http://dx.doi.org/10.31274/cc-20240624-319.

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Mira, Joan F. Assaig breu sobre Fuster. Universitat de València, February 2023. http://dx.doi.org/10.7203/puv-esp67-17-mira.

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Evan B. Douple. Final Report - BRER Core Support. Office of Scientific and Technical Information (OSTI), January 2007. http://dx.doi.org/10.2172/897000.

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Ellis, Christopher D., Byoung-Suk Kweon, and Mark Storie. Brent Elementary Schoolyard Greening: Phase 1. Landscape Architecture Foundation, 2012. http://dx.doi.org/10.31353/cs0310.

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Ibe, M. Breit-Wigner Enhancement of Dark Matter Annihilation. Office of Scientific and Technical Information (OSTI), June 2009. http://dx.doi.org/10.2172/957441.

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Pessaro, Brian. Evaluation of HART MetroRapid BRT. Tampa, FL: University of South Florida, August 2015. http://dx.doi.org/10.5038/cutr-nctr-rr-2015-09.

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Nelson, Arthur. National Study of BRT Development Outcomes. Portland State University Library, November 2015. http://dx.doi.org/10.15760/trec.28.

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Al Sadoon, Hamid, Colin Ward, Jennifer Considine, and Abdullah Al Dayel. A Short-Term Forecasting Model for Brent Oil Prices. King Abdullah Petroleum Studies and Research Center, October 2019. http://dx.doi.org/10.30573/ks--2019-mp08.

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Corsetto, Lisa, César P. Bouillon, Daniel Oviedo, Lynn Scholl, and Cheryl Gray. Approach Paper: An Evaluation of the Effects of IDB Supported BRT Systems on Mobility and Access for the Poor in Cali and Lima. Inter-American Development Bank, March 2016. http://dx.doi.org/10.18235/0010640.

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Abstract:
Bus rapid transit (BRT) systems have become an increasingly popular approach to addressing mobility and environmental problems in urban areas in Latin America and around the world. Building on OVE's recent evaluation, this analysis of BRT Systems and Poverty in Cali and Lima aims to expand the aforementioned evaluation of the BRT project results with respect to their objectives of improving mobility and access for the poor.
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