Academic literature on the topic 'Bos Reproduction'
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Journal articles on the topic "Bos Reproduction"
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Kwatra, M. S., and Ajit Singh. "Experimental Reproduction of Gangrenous Syndrome in Buffaloes (Bos bubalus)." Zentralblatt für Veterinärmedizin Reihe B 20, no. 6 (May 13, 2010): 481–89. http://dx.doi.org/10.1111/j.1439-0450.1973.tb01151.x.
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Davis, GP. "Genetic parameters for tropical beef cattle in northern Australia: a review." Australian Journal of Agricultural Research 44, no. 2 (1993): 179. http://dx.doi.org/10.1071/ar9930179.
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This paper reviews published estimates of genetic parameters for traits of growth, reproduction and resistance to environmental stresses for Bos indicus and Zebu derived breeds in northern Australia. Most published estimates of heritabilities for growth and reproduction traits were higher for tropically adapted breeds in northern Australia than for Bos taurus breeds in temperate Australia. Weighted mean estimates of heritabilities for the direct component of weaning weight were 39% for the Brahman breed and 30% for Zebu-derived breeds in northern Australia compared with 13% for Bos taurus breeds in temperate areas of Australia. Mean estimates for the maternal component of weaning weight were 5, 24 and 13% respectively. Mean heritabilities for yearling and 550 day weights for Zebu derived breeds in northern Australia (24 and 25%) were similar to those for Bos taurus breeds in temperate areas, though estimates for Brahmans were higher (39 and 39%). Published estimates of heritabilities of later weights (700 and 900 days), which are most relevant to northern Australian production systems, were rare but averaged between 32 and 45% for Zebu-derived breeds and Brahmans. Weighted mean heritability for female calving success was 14% and for realised bull fertility was 5%. Published estimates of heritabilities of scrota1 circumference averaged 31%, and testosterone response to GNRH stimulation was 52%. Heritabilities of resistance to various environmental stresses were all moderate with weighted means between 20 and 34%. Genetic correlations between growth, reproduction and resistance to environmental stresses are also reviewed. There appears to be predictable variation in estimates of parameters between breeds in different environments which is related to level of resistance to environmental stresses, and this is likely to affect the prediction of breeding values for cattle in northern Australia.
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Zi, Xiang-Dong. "Reproduction in female yaks (Bos grunniens) and opportunities for improvement." Theriogenology 59, no. 5-6 (March 2003): 1303–12. http://dx.doi.org/10.1016/s0093-691x(02)01172-x.
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CHENOWETH, PJ. "Aspects of reproduction in female Bos indicus cattle: a review." Australian Veterinary Journal 71, no. 12 (December 1994): 422–26. http://dx.doi.org/10.1111/j.1751-0813.1994.tb00961.x.
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Suarez, S. S. "Interactions of spermatozoa with the female reproductive tract: inspiration for assisted reproduction." Reproduction, Fertility and Development 19, no. 1 (2007): 103. http://dx.doi.org/10.1071/rd06101.
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Artificial insemination with sexed semen, in vitro fertilisation and intracytoplasmic sperm injection have been used to reproduce animals, but often not as successfully as natural mating. Learning more about how spermatozoa normally interact with the female tract can provide inspiration for developing improvements in assisted reproduction. The present review focuses on Bos taurus, because more is known about this species than others. At coitus, bull spermatozoa are deposited into the anterior vagina, where they rapidly enter the cervix. Cervical mucus quickly filters out seminal plasma from spermatozoa, unlike most assisted reproduction protocols. Spermatozoa that reach the uterus may require certain cell surface proteins to swim through the uterotubal junction. Shortly after passing through the junction, most spermatozoa are trapped in a storage reservoir by binding to oviducal epithelium, in the case of cattle via bovine seminal plasma (BSP) proteins coating the sperm head. As ovulation approaches, spermatozoa capacitate and shed BSP proteins. This reduces sperm binding to the epithelium and releases them from storage. Motility hyperactivation assists spermatozoa in leaving the storage reservoir, swimming through oviducal mucus and the cumulus oophorus, and penetrating the oocyte zona pellucida. Chemotactically regulated switching between asymmetrical (i.e. hyperactivated) and symmetrical flagellar beating may also guide spermatozoa to the oocyte.
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Ali, Sayid, Tamrat Degefa, and Alemayehu Lemma. "Morphometrical study of the reproductive tract in Boran (Bos indicus) heifers in Ethiopia." Ethiopian Veterinary Journal 25, no. 2 (September 6, 2021): 1–13. http://dx.doi.org/10.4314/evj.v25i2.1.
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Boran breed is the most suitable type of cattle breed for arid and semi-arid regions of Ethiopia due to their adaptive characteristics. Understanding their reproductive anatomy is important for successful reproductive management and has many implications for the application of assisted reproductive technology, and interpretation of physiological changes at different stages of their reproductive cycle. In this study, data of the anatomical structures of the reproductive organs of 20 Boran heifers were measured and characterized at different stages of reproduction. The mean (±SE) length (L) of the vagina, cervix, and body of uterus were 13.17±2.98 cm, 7.19±1.28 cm, and 6.70±1.08cm, respectively. The mean (±SE) length of the right uterine horn and oviduct was 18.47 ±2.54 and 18.17 ±1.27cm, respectively and that of the left were 18.05±2.73 and 17.45±1.80 cm, respectively. The mean ±SE weight (W) of right and left ovaries were 2.44±1.51 gm and 2.44±1.51 gm, respectively. The mean ±SE Length x Width x Thickness of the right ovary was 2.10±0.59 x 1.75±0.35 x 0.83±0.36 and that of the left was 1.97±0.41 x 1.58±0.29 x 0.67±0.33 cm. The mean (±SE) weight of the whole reproductive organ (without the vulva) was 301.35±66.34gm. The ovarian size was influenced by the presence of the corpus luteum(CL) usually larger with its presence.
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UTTAM, VISHAKHA, MADHU TIWARI, SATYENDRA PAL SINGH, DEEPAK SHARMA, AVNEESH KUMAR, and ADITYA KUMAR. "Molecular characterization and association studies of CYP19 (aromatase) allelic variants with reproduction and production traits in Sahiwal and Hariana cattle." Indian Journal of Animal Sciences 92, no. 2 (March 10, 2022): 202–7. http://dx.doi.org/10.56093/ijans.v92i2.122094.
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Anestrus is a major reproductive problem in bovines which occurs due to less secretion of estrogen from the ovarian follicles. Enzyme cytochrome P450 aromatase which helps in estrogen biosynthesis is encoded by CYP19 gene. In the current study, characterization of exon 9 and 10 region and polymorphic study in 5' UTR region of CYP19 gene was undertaken in Indian Sahiwal and Hariana cattle. Studied Indian cattle breeds showed 100% homology to Nelore and crossbred cattle, and 99.8% to Bos taurus at nucleotide level while at amino acid level, they showed 100% homology to Nelore and crossbred cattle, and 99.4% to Bos taurus. CYP19/PvuII PCR-RFLP assay conducted in 100 Sahiwal and 100 Hariana cattle revealed three types of genotype namely AA (405 bp), AB (405 bp, 327 bp and 78 bp) and BB (327 bp and 78 bp). The AA genotype was more frequent (58.5%) followed by AB (37.0%) and BB (4.5%) genotypes and Chi square analysis revealed the populations was in Hardy-Weinberg equilibrium. The allelic frequency of A and B allele was observed as 0.77 and 0.23, respectively. Association study of CYP19/PvuII genotypes with reproduction and production traits revealed no significant difference.
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Perumal, P., K. Vupru, K. Khate, M. Veeraselvam, Atul Kumar Verma, A. K. Nahak, and C. Rajkhowa. "Blood profiles of mithun (Bos frontalis) under different stages of reproduction." International Journal of Bio-resource and Stress Management 5, no. 1 (2014): 064. http://dx.doi.org/10.5958/j.0976-4038.5.1.011.
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RAMACHANDRAN, S., M. MALOLE, D. RIFULIADI, and T. SAFRIATI. "EXPERIMENTAL REPRODUCTION OF MALIGNANT CATARRHAL FEVER IN BALI CATTLE (BOS SONDAICUS)." Australian Veterinary Journal 58, no. 4 (March 10, 2008): 169–70. http://dx.doi.org/10.1111/j.1751-0813.1982.tb00642.x.
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Ferraz, J. B. S., X. L. Wu, H. Li, J. Xu, R. Ferretti, B. Simpson, J. Walker, et al. "Development and evaluation of a low-density single-nucleotide polymorphism chip specific to Bos indicus cattle." Animal Production Science 60, no. 15 (2020): 1769. http://dx.doi.org/10.1071/an19396.
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Context Genomic selection has been of increasing interest in the genetic improvement of Zebu cattle, particularly for quantitative traits that are difficult or expensive to measure, such as carcass traits and meat tenderness. The success of genomic selection depends on several factors, and at its core is the availability of single-nucleotide polymorphism (SNP) chips that are appropriately designed for Bos indicus cattle. However, the currently available commercial bovine SNP chips are mostly designed for Bos taurus cattle. There are two commercial Bos indicus SNP chips; namely, GeneSeek genomic profiler high-density Bos indicus (GGP-HDi) SNP chip and a low-density (LD) Bos indicus SNP chip (Z chip), but these two Bos indicus SNP chips were built with mixed contents of SNPs for Bos indicus and Bos taurus cattle, due to limited availability of genotype data from Bos indicus cattle. Aims To develop a new GGP indicus 35000 SNP chip specifically for Bos indicus cattle, which has a low cost, but high accuracy of imputation to Illumina BovineHD chips. Methods The design of the chip consisted of 34000 optimally selected SNPs, plus 1000 SNPs pre-reserved for those on the Y chromosome, ‘causative’ mutations for a variety of economically relevant traits, genetic health conditions and International Society for Animal Genetics globally recognised parentage markers for those breeds of cattle. Key results The present results showed that this new indicus LD SNP chip had considerably increased minor allele frequencies in indicus breeds than the previous Z-chip. It demonstrated with high imputation accuracy to HD SNP genotypes in five indicus breeds, and with considerable predictability on 14 growth and reproduction traits in Nellore cattle. Conclusions This new indicus LD chip represented a successful effort to leverage existing knowledge and genotype resources towards the public release of a cost-effective LD SNP chip specifically for Bos indicus cattle, which is expected to replace the previous GGP indicus LD chip and to supplement the existing GGP-HDi 80000 SNP chip. Implications A new SNP chip specifically designed for Bos indicus, with high power of imputation to Illumina BovineHD technology and with excellent coverage of the whole genome, is now available on the market for Bos indicus cattle, and Bos indicus and Bos taurus crosses.
Dissertations / Theses on the topic "Bos Reproduction"
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Rodrigues, Adnan Darin Pereira 1987. "Desempenho reprodutivo em novilhas Bos indicus e Bos taurus X Bos indicus submetidas a protocolos de sincronização da ovulação." Botucatu, 2016. http://hdl.handle.net/11449/148021.
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Orientador: José Luiz Moraes VasconcelosResumo: O objetivo dessa tese foi avaliar o desempenho reprodutivo de novilhas Bos indicus (BI) e Bos taurus x Bos indicus (CR) submetidas a protocolos de sincronização da ovulação. No Capitulo 2, novilhas BI e CR púberes e pré-púberes foram submetidas a estratégia reprodutiva proposta por Rodrigues et al. (2014) e um grupo de novilhas púberes foram sincronizadas com o protocolo de IATF padrão. No Capítulo 3, novilhas BI e CR foram submetidas a estratégia reprodutiva aos 12 meses de idade. No Capítulo 4, foi avaliado o efeito da concentração circulante de P4 no Dia 9 do protocolo de IATF e tratamento com eCG em novilhas BI e CR. No experimento do Capítulo 2 uma maior porcentagem de novilhas BI estavam pré-púberes no início do experimento comparadas as novilhas do grupo CR. A taxa de indução não foi diferente entre novilhas BI e novilhas CR. A raça interferiu na prenhez à IATF na Fazenda 1, mas não interferiu na Fazenda 2. Novilhas que não responderam ao protocolo de indução tiveram menor taxa de prenhez à IATF em relação aos demais grupos experimentais. No experimento do Capítulo 3, a presença de CL no D0 não diferiu entre os grupos genéticos. Animais mais pesados apresentaram maior presença de CL, independentemente de raça. Houve efeito de CL no D0 na taxa de prenhez à IATF. Não foi detectada interação entre presença de CL e raça na prenhez, sendo que novilhas BI tiveram menor P/IA do que as novilhas CR. Houve interação entre peso e CL no D0 na P/IA, sendo que em novilhas com CL no... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Not available
Doutor
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Gladney, Cody Jack. "Evaluation of udder and teat characteristics, calf growth, and reproduction in young Bos indicus-Bos taurus cows." Texas A&M University, 2008. http://hdl.handle.net/1969.1/85988.
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Sire and family effects were evaluated for calf growth, udder and teat
conformation, and reproduction traits in 2- to 4- yr-old cows from the McGregor
Genomics Project. Cows were produced by embryo transfer (ET) and natural service
(NS) from the same 4 F1 Nellore-Angus sires, and were analyzed separately. Sire of cow
was significant for calf birth weight (P = 0.014) among ET cows, but not NS cows.
Among NS families, calves from cows out of Brahman-Hereford dams were 2.0 kg
heavier (P = 0.064) at birth than calves from cows out of Brahman-Angus dams. Sire of
cow accounted for variation in weaning weight (P = 0.006) and preweaning ADG (P =
0.005) of calves from ET dams, but not NS dams. Family nested within sire also
accounted for variation (P = 0.061) in weaning weights of calves from ET dams. Sire of
cow was significant for average teat length in ET (P < 0.001) and NS (P = 0.013) cows.
Sire of cow was significant for average teat diameter (P = 0.022) among NS cows. Sire
of cow also affected udder support score (P = 0.002), cow disposition at calf birth (P =
0.002), and cow weight at weaning (P = 0.045) in ET cows. Family and cow age also
accounted for variation in cow disposition at calf birth (P = 0.015, P = 0.041, respectively) and cow weight at weaning (P = 0.001, P < .001, respectively) among ET
cows. Calf year of birth also affected (P < .001) cow weight at weaning among ET
cows. For NS dams, calf year of birth (P = 0.012), cow age (P < .001), and parity nested
within cow age (P = 0.005) affected cow weight at weaning. Although reproduction
data were not formally analyzed, there appear to be substantial differences for calving
rate and average calving date among these cow families. Data from this project will be
used for identification of genetic markers for these cow productivity traits.
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Verdurico, Lenita Camargo. "Efeito da suplementação prolongada de ácidos graxos insaturados na alimentação de novilhas Bos taurus sobre a qualidade oocitária e embrionária e perfil metabólico." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/10/10135/tde-14092015-095229/.
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Objetivou-se avaliar os efeitos de diferentes fontes de ácidos graxos essenciais, ômega 3 e ômega 6, e o tempo de suplementação sobre a qualidade oocitária e embrionária em novilhas Bos taurus. Foram selecionadas 24 novilhas da raça Holandesa, divididas em três grupos experimentais os quais receberam as seguintes dietas: 1) Controle (CT) composto por dieta basal de aproximadamente 2,5% de extrato etéreo; 2) Grão de Soja (GS) composto por uma dieta com aproximadamente 4,5% de extrato etéreo, obtido com a inclusão de 11,5% de grão de soja cru e integral na matéria seca (MS) da dieta, sendo fonte de ômega 6; 3) Semente de Linhaça (SL), composto por uma dieta com aproximadamente 4,5% de extrato etéreo, baseada na inclusão de 6,0% de semente de linhaça na MS da dieta, sendo fonte de ômega 3. Os animais foram arraçoados em grupos, de acordo com o consumo de matéria seca do dia anterior de forma a ser mantido porcentual de sobras diárias, entre 5 e 10% do consumo. Foi avaliada, por ultrassonografia, a atividade ovariana de todos os animais durante todo período de coleta. Foram realizadas aspirações foliculares precedidas de sincronização da emergência de onda de crescimento folicular em seis períodos, -30, 0, 30, 60, 90 e 120 dias, os oócitos recuperados foram classificados de acordo com sua morfologia. Foram considerados como viáveis e, consequentemente aproveitados para FIV somente os oócitos classificados como graus I, II e III, os demais foram descartados. Os animais foram pesados para mensuração de ganho médio de peso diário, e amostras de fluido folicular do folículo dominante e sangue foram coletadas concomitante às aspirações foliculares a fim de se avaliar os parâmetros metabólicos como glicose, colesterol total, colesterol HDL, colesterol VLDL, colesterol LDL, triglicerídeos e ureia, Houve efeito de tempo para o número de oócitos classificados em Grau I, número e taxa de embriões. Na avaliação dos contrastes ortogonais, foi observado efeito para o contraste 1 (CT vs GS+SL) quando se analisou a taxa de embriões viáveis, onde a dieta CT apresentou menor desempenho. Não foi observado efeito significativo dos tratamentos para ganho de peso diário e peso corporal das novilhas, onde o ganho de peso médio diário (GPD) entre os tratamentos foi de 1072 gramas para os animais do grupo controle, 972 gramas para o grupo tratado com grão de soja e 840 gramas para suplementação com semente de linhaça. Houve efeito de tempo para a pesagem dos animais alimentados com as dietas experimentais, em que foi observado um aumento significativo de peso dos animais. Para o ganho de peso diário (GPD) foi observado efeito para o contraste 1, em que os animais tratados com a dieta controle apresentaram maiores ganho de peso que os animais alimentados com dietas suplementadas com lipídeos. As duas fontes lipídicas avaliadas não diferiram entre si. Houve efeito de tempo para todas as variáveis do perfil metabólico sanguíneo analisadas ao longo de todo período experimental. Foi observado efeito de interação entre tempo e dieta para a variável glicose. Na análise dos contrastes foi observado efeito para o C1 (controle vs grão de soja + semente de linhaça) onde os animais que consumiram dieta controle apresentaram em média 152,29 mg/dL de glicose em relação aos que consumiram dietas suplementadas com fontes de ácidos graxos (GS e SL) (141,74 + 144,78) respectivamente. Houve efeito estatístico para interação entre tempo e dieta para os níveis de ureia. Na análise do fluído folicular foi observado interação entre tempo e dieta para as variáveis colesterol total e ureia. Foram observados efeitos significativos de dieta, interação tempo dieta e contraste 1 (CT vs GS + SL) quando se analisou a quantidade de triglicerídeos no fluído folicular. Portanto a suplementação prolongada com fontes de ácidos graxos insaturados na alimentação de novilhas não apresenta melhorias consideráveis na qualidade oocitária e embrionária dos animais, aumentando apenas a taxa de embriões viáveis, mas com prejuízos sobre o perfil metabólico e ganho médio de peso vivo.This study aimed to evaluate the effects of different sources of essential fatty acids, omega 3 and omega 6, and the time of supplementation on oocyte and embryo quality in Holstein heifers. Will be selected 30 heifers Bos taurus, evenly divided into three groups which will receive the following diets: 1) Control (CT) basal diet composed of approximately 2,5% ethereal stratum; 2) Soya Bean (GS) consists of a diet with approximately 4,5% of ethereal layer based on inclusion of 12% of raw soybean and complete the concentrate, a source of omega 6; 3) Flax Seed (SL) composed of a diet with approximately 4,5% ethereal stratum based on inclusion of 6,0% of flaxseed in the concentrate, a source of omega 3. The animals are hand fed into groups according to the dry matter intake inside the day in order to be kept remains percentage of the diets on a daily basis between 5 and 10%. Will be evaluated by ultrasound ovarian activity of all animals throughout the collection period. Be preceded by follicular aspiration performed synchronization emergency follicular wave into six periods, - 30, 0, 30, 60, 90 and 120 days, the oocytes retrieved will be ranked according to their morphology, by measuring the amount of layers and compacting cumulus cells and homogeneity of the ooplasm. Are considered viable and therefore only the recovered oocytes for IVF classified as grade I, II and III, the other is discarded. Follicular fluid samples of the dominant follicle and the concomitant blood will be collected follicular aspiration in order to evaluate the metabolic and hormonal parameters such as glucose, total cholesterol, HDL cholesterol, VLDL cholesterol, LDL cholesterol, triglycerides, and urea. As expected outcome of the present study suggest that supplementation with fat has beneficial effects on follicle, the oocyte quality, the quality of in vitro embryo production and metabolic and hormonal profile of dairy heifers. There was a time effect on the number of oocytes classified as Class I, number and rate of embryos. In assessing orthogonal contrasts, effect was observed for the first contrast (CT vs GS + SL) when analyzing the rate of viable embryos, where the CT diet showed lower performance. There was no significant treatment effect on daily weight gain and body weight of heifers, where the average daily gain weight (GPD) between treatments was 1072 grams for the control group, 972 grams for the group treated with grain of soybeans and 840 grams for supplementation with flaxseed. There was a time effect for weighing animals fed experimental diets, it was observed a significant increase in initial weight to the final weight of the treated animals. In assessing orthogonal contrasts, effect was observed for the first contrast (CT vs GS + SL) for the GPD (daily weight gain) in the animals treated with the control diet showed higher values for average found weight gain, compared to diets with added lipid. However, when we analyzed the contrast 2 (GS vs SL) was not observed statistical effect of the tested variables. There was a time effects for all variables examined the metabolic profile of blood throughout the experimental period. There was a significant interaction effect between time and diet to the variable glucose. In the analysis of contrasts effect was observed for C1 (control versus soybean grain, flaxseed) where the animals fed the control diet had an average of 152.29 mg / dL glucose compared to those fed diets supplemented with acid sources fatty (GS and SL) (141.74 + 144.78) respectively. There was no effect for interaction between time and diet for urea. In the analysis of follicular fluid was observed interaction between time and diet for the variables Total cholesterol and urea. Diet Significant effects were observed, time and diet interaction Contrast 1 (CT vs. GS + SL) where it is examined the amount of triglycerides in the follicular fluid. Therefore prolonged supplementation sources of unsaturated fatty acids in the diet of heifers not considerably improvements in oocyte and embryo quality animal, only increasing the rate of viable embryos, but with losses on the metabolic profile and average live weight gain.
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Ticianelli, Janahi Sousa [UNESP]. "Uso de microarray para determinação da expressão gênica diferencial em oócitos Bos Taurus Indicus e Bos Taurus Taurus submetidos ao estresse térmico." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/123297.
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000827001.pdf: 1244164 bytes, checksum: 53be6cbd163a4c6a3a3afa2dbf6acc42 (MD5)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Divergências genéticas de termotolerância foram demonstradas entre animais Bos taurus taurus (Holandesa) e Bos taurus indicus (Nelore). O efeito deletério do estresse térmico sobre o potencial de desenvolvimento do oócito é maior para Bos taurus taurus em comparação aos oócitos Bos taurus indicus. Portanto, o presente estudo determinou o perfil do transcriptoma em oócitos das raças Nelore e Holandesa submetidos ao estresse térmico durante a maturação in vitro (MIV), bem como a abundância de RNAm de genes candidatos em oócitos e células do cumulus submetidos aos mesmos tratamentos. Vacas não lactantes das raças Holandesa e Nelore foram submetidas à aspiração folicular guiada por ultra-som durante a estação fria. Os complexos cumulus-oócitos (CCOs) foram aleatoriamente distribuídos nos tratamentos controle (38,5 °C por 22 horas) e estresse térmico (41 °C por 12 horas, seguido de 38,5 °C por 10 horas) durante a MIV. Oócitos desnudados foram submetidos à análise de microarray bovino (Affymetrix). Genes com fold change de pelo menos 1,5 e P<0,05 foram considerados diferencialmente expressos. A análise de microarray demonstrou 127, 9 e 6 genes diferencialmente expressos entre Raça, Temperatura e interação Raça x Temperatura, respectivamente. Os genes diferencialmente expressos foram avaliados por RT-PCR em oócitos e nas suas respectivas células de cumulus. Houve uma correlação positiva entre o fold change do microarray e do RT-PCR dos oócitos para os genes KIF3A, CLDN11, BIRC3, DAP, MT1E, CCT4, DICER1 e DENND3. Em particular, o gene membro da família cinesina 3A (KIF3A) foi induzido em oócitos de Holandesa, enquanto que os genes da proteína associada à morte (DAP) e de tráfego intracelular de membrana domínio DENN/MADD 3 (DENND3) foram reprimidos em oócitos de Holandesa quando comparados a Nelore. A abundância relativa do RNAm da molécula antioxidante metalotioneína 1E ...
Genetic divergences in thermotolerance have been demonstrated between Bos taurus taurus (Holstein) and Bos taurus indicus (Nelore) animals. The deleterious effect of heat stress on oocyte developmental potential is greater for Bos taurus taurus as compared to Bos taurus indicus oocytes. Therefore, the present study determined transcriptome profile in Nelore and Holstein oocytes subjected heat shock during in vitro maturation (IVM) as well as mRNA abundance of selected candidate genes in Nelore and Holstein heat-shocked oocytes and cumulus cells. Non-lactating Holstein and Nelore cows were subjected to ultrasound-guided follicle aspiration during the cool season. Cumulus-oocyte complexes (COCs) were randomly assigned to control (38.5°C for 22 hours) and heat shock (41°C for 12 hours followed by 38.5°C for 10 hours) treatments during IVM. Denuded oocytes were subjected to Affymetrix bovine microarray. Genes with fold change of at least 1.5 and P< 0.05 were considered differently expressed. Microarray analyses demonstrated 127, 9 and 6 genes differentially expressed between Breed, Temperature and Breed x Temperature interaction, respectively. Selected differentially expressed genes were evaluated by RTPCR in oocytes and respective cumulus cells. There was a positive correlation between oocyte microarray and RT-PCR fold change for the genes KIF3A, CLDN11, BIRC3, DAP, MT1E, CCT4, DICER1 and DENND3. In particular, the member of the kinesin family 3A (KIF3A) gene was up-regulated in Holstein oocytes, while the deathassociated protein (DAP) and the protein trafficking gene DENN/MADD domain containing 3 (DENND3) were down-regulated in Holstein oocytes as compared to Nelore. The mRNA relative abundance of the antioxidant molecule metallothionein 1E (MT1E) was higher in Holstein cumulus cells, whereas, claudin 11 (CLDN11) that participates in cellular tight junctions, was higher in Nelore. Moreover, heat shock down regulated MT1E mRNA abundance ...
FAPESP: 2007/53323-0
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Ticianelli, Janahi Sousa. "Uso de microarray para determinação da expressão gênica diferencial em oócitos Bos Taurus Indicus e Bos Taurus Taurus submetidos ao estresse térmico /." Botucatu, 2014. http://hdl.handle.net/11449/123297.
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Orientador: Fabíola de Paula LopesBanca: Gisele Ziccal Mingoti
Banca: José Antônio Visintin
Banca: Marcelo Fábio Gouveia Nogueira
Banca: Fernanda da Cruz Landim
Resumo: Divergências genéticas de termotolerância foram demonstradas entre animais Bos taurus taurus (Holandesa) e Bos taurus indicus (Nelore). O efeito deletério do estresse térmico sobre o potencial de desenvolvimento do oócito é maior para Bos taurus taurus em comparação aos oócitos Bos taurus indicus. Portanto, o presente estudo determinou o perfil do transcriptoma em oócitos das raças Nelore e Holandesa submetidos ao estresse térmico durante a maturação in vitro (MIV), bem como a abundância de RNAm de genes candidatos em oócitos e células do cumulus submetidos aos mesmos tratamentos. Vacas não lactantes das raças Holandesa e Nelore foram submetidas à aspiração folicular guiada por ultra-som durante a estação fria. Os complexos cumulus-oócitos (CCOs) foram aleatoriamente distribuídos nos tratamentos controle (38,5 °C por 22 horas) e estresse térmico (41 °C por 12 horas, seguido de 38,5 °C por 10 horas) durante a MIV. Oócitos desnudados foram submetidos à análise de microarray bovino (Affymetrix). Genes com fold change de pelo menos 1,5 e P<0,05 foram considerados diferencialmente expressos. A análise de microarray demonstrou 127, 9 e 6 genes diferencialmente expressos entre Raça, Temperatura e interação Raça x Temperatura, respectivamente. Os genes diferencialmente expressos foram avaliados por RT-PCR em oócitos e nas suas respectivas células de cumulus. Houve uma correlação positiva entre o fold change do microarray e do RT-PCR dos oócitos para os genes KIF3A, CLDN11, BIRC3, DAP, MT1E, CCT4, DICER1 e DENND3. Em particular, o gene membro da família cinesina 3A (KIF3A) foi induzido em oócitos de Holandesa, enquanto que os genes da proteína associada à morte (DAP) e de tráfego intracelular de membrana domínio DENN/MADD 3 (DENND3) foram reprimidos em oócitos de Holandesa quando comparados a Nelore. A abundância relativa do RNAm da molécula antioxidante metalotioneína 1E ...
Abstract: Genetic divergences in thermotolerance have been demonstrated between Bos taurus taurus (Holstein) and Bos taurus indicus (Nelore) animals. The deleterious effect of heat stress on oocyte developmental potential is greater for Bos taurus taurus as compared to Bos taurus indicus oocytes. Therefore, the present study determined transcriptome profile in Nelore and Holstein oocytes subjected heat shock during in vitro maturation (IVM) as well as mRNA abundance of selected candidate genes in Nelore and Holstein heat-shocked oocytes and cumulus cells. Non-lactating Holstein and Nelore cows were subjected to ultrasound-guided follicle aspiration during the cool season. Cumulus-oocyte complexes (COCs) were randomly assigned to control (38.5°C for 22 hours) and heat shock (41°C for 12 hours followed by 38.5°C for 10 hours) treatments during IVM. Denuded oocytes were subjected to Affymetrix bovine microarray. Genes with fold change of at least 1.5 and P< 0.05 were considered differently expressed. Microarray analyses demonstrated 127, 9 and 6 genes differentially expressed between Breed, Temperature and Breed x Temperature interaction, respectively. Selected differentially expressed genes were evaluated by RTPCR in oocytes and respective cumulus cells. There was a positive correlation between oocyte microarray and RT-PCR fold change for the genes KIF3A, CLDN11, BIRC3, DAP, MT1E, CCT4, DICER1 and DENND3. In particular, the member of the kinesin family 3A (KIF3A) gene was up-regulated in Holstein oocytes, while the deathassociated protein (DAP) and the protein trafficking gene DENN/MADD domain containing 3 (DENND3) were down-regulated in Holstein oocytes as compared to Nelore. The mRNA relative abundance of the antioxidant molecule metallothionein 1E (MT1E) was higher in Holstein cumulus cells, whereas, claudin 11 (CLDN11) that participates in cellular tight junctions, was higher in Nelore. Moreover, heat shock down regulated MT1E mRNA abundance ...
Doutor
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Risolia, Pedro Henrique Bugallo [UNESP]. "O efeito do fator de crescimento semelhante à insulina-I em oócitos de vacas Bos indicus e Bos taurus expostas ao estresse térmico un vitro." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/108541.
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000753005.pdf: 2398846 bytes, checksum: 88a9bdc09aaab4eb0166b0e572c83bb7 (MD5)As alterações no microambiente do trato reprodutivo comprometem a competência oocitária e o desenvolvimento embrionário pré-implantacional em bovinos. Vacas Bos indicus apresentam adaptações fenotípicas e genotípicas conferindo maior resistência e tolerância à temperatura elevada quando comparadas as vacas Bos taurus, que sofrem redução nos índices de prenhez durante os meses quentes do ano. Esta infertilidade causada pelo estresse térmico é um problema de ordem multifatorial, entretanto já foi demonstrado que o oócito e embrião são extremamente susceptíveis aos efeitos deletérios da temperatura elevada. Os danos celulares desencadeados pela temperatura elevada podem ser manipulados pelo fator de crescimento semelhante à insulina-I (IGF-I), que além de minimizar a morte celular espontânea, resgata células que já iniciaram apoptose induzida por diferentes agentes ou estresses. A primeira etapa deste projeto visou avaliar o efeito dose resposta do IGF-I durante a maturação in vitro (MIV) em meio quimicamente não definido (pré-experimento 1: meio MIV - 10% de soro fetal bovino) e semidefinido (pré-experimento 2: meio MIV - 6 mg/mL de albumina sérica bovina) na competência de desenvolvimento de oócitos bovinos. Para tanto, complexos cumulus-oócito (CCOs) de vacas (mestiças Bos indicus) oriundas de abatedouro foram maturados in vitro em meio MIV não definido ou semidefinido suplementado com 0 (controle veículo), 6,25; 12,5; 25; 50; 100 ou 200 ng/mL de IGF-I por 22-24 h. Em seguida os oócitos foram fecundados (FIV) e cultivados in vitro (CIV). A adição de 25 ng/mL de IGF-I no meio MIV semidefinido ou não definido aumentou a porcentagem de oócitos clivados no dia 3 e a taxa de blastocisto no dia 8 após a fecundação. Dessa forma, a dose de 25 ng/mL de IGF-I foi utilizada na segunda etapa deste projeto, cujo objetivo foi avaliar o papel do IGF-I na competência de desenvolvimento de ...
Microenvironmental alterations on reproductive tract compromise oocyte competence and pre-implicational embryo development in bovine. This fact is well characterized in animals exposed to heat stress. Bos indicus have fenotypic and genotypic adaptations which confers a higher tolerance to high temperatures compared with Bos taurus, suffering a reduction in the pregnancy rates during the hot months. This infertility caused by heat stress is a multifactorial problem, however it has been shown that oocyte and embryo are very susceptible to deleterious effects of high temperature. The cellular damage induced by high temperature could be manipulated by insulin-like growth factor I (IGF-I), which besides minimizing the spontaneous cell death, IGF-I rescue cells which have already initiated the apoptosis induced by different agents or stresses. The first step of this project aimed to evaluate dose-response effect of IGF-I during the maturation in vitro (MIV) in a non-defined medium (pre-experiment 1: MIV - 10% of bovine fetal serum) and semi-defined (preexperiment 2: MIV - 6 mg/mL of bovine albumin serum) in competence of bovine oocytes. Therefore, cumulus-oocyte complexes (CCOs) obtained of cows (Bos indicus crossbreed) from a slaughterhouse were matured in vitro in non-defined and semidefined MIV medium supplemented with 0 (vehicle control), 6,25; 12,5; 25; 50; 100 or 200 ng/mL of IGF-I for 22-24 h. After, oocytes were fertilized (FIV) and cultured in vitro (CIV). The adition of 25 ng/ml of IGF-I on non-defined and semi-defined MIV medium increased porcentage of cleavage on day 3 and blastocysts rate in day 8 after fertilization. In this way, 25 ng/ml IGF-I was used on second step of this experiment, which objective was to evaluate the role of IGF-I on oocyte competence of oocytes collected from Bos indicus – Nelore (NEL; n= 6) and Bos taurus-Holstein (HPB; n= 6) exposed to heat stress. Therefore, cows adapted ...
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Risolia, Pedro Henrique Bugallo. "O efeito do fator de crescimento semelhante à insulina-I em oócitos de vacas Bos indicus e Bos taurus expostas ao estresse térmico un vitro /." Botucatu, 2013. http://hdl.handle.net/11449/108541.
Full textAbstract:
Orientador: Fabíola Freitas de Paula LopesBanca: José Antônio Visintin
Banca: Marcelo Fábio Gouvia Nogueira
Resumo: As alterações no microambiente do trato reprodutivo comprometem a competência oocitária e o desenvolvimento embrionário pré-implantacional em bovinos. Vacas Bos indicus apresentam adaptações fenotípicas e genotípicas conferindo maior resistência e tolerância à temperatura elevada quando comparadas as vacas Bos taurus, que sofrem redução nos índices de prenhez durante os meses quentes do ano. Esta infertilidade causada pelo estresse térmico é um problema de ordem multifatorial, entretanto já foi demonstrado que o oócito e embrião são extremamente susceptíveis aos efeitos deletérios da temperatura elevada. Os danos celulares desencadeados pela temperatura elevada podem ser manipulados pelo fator de crescimento semelhante à insulina-I (IGF-I), que além de minimizar a morte celular espontânea, resgata células que já iniciaram apoptose induzida por diferentes agentes ou estresses. A primeira etapa deste projeto visou avaliar o efeito dose resposta do IGF-I durante a maturação in vitro (MIV) em meio quimicamente não definido (pré-experimento 1: meio MIV - 10% de soro fetal bovino) e semidefinido (pré-experimento 2: meio MIV - 6 mg/mL de albumina sérica bovina) na competência de desenvolvimento de oócitos bovinos. Para tanto, complexos cumulus-oócito (CCOs) de vacas (mestiças Bos indicus) oriundas de abatedouro foram maturados in vitro em meio MIV não definido ou semidefinido suplementado com 0 (controle veículo), 6,25; 12,5; 25; 50; 100 ou 200 ng/mL de IGF-I por 22-24 h. Em seguida os oócitos foram fecundados (FIV) e cultivados in vitro (CIV). A adição de 25 ng/mL de IGF-I no meio MIV semidefinido ou não definido aumentou a porcentagem de oócitos clivados no dia 3 e a taxa de blastocisto no dia 8 após a fecundação. Dessa forma, a dose de 25 ng/mL de IGF-I foi utilizada na segunda etapa deste projeto, cujo objetivo foi avaliar o papel do IGF-I na competência de desenvolvimento de ...
Abstract: Microenvironmental alterations on reproductive tract compromise oocyte competence and pre-implicational embryo development in bovine. This fact is well characterized in animals exposed to heat stress. Bos indicus have fenotypic and genotypic adaptations which confers a higher tolerance to high temperatures compared with Bos taurus, suffering a reduction in the pregnancy rates during the hot months. This infertility caused by heat stress is a multifactorial problem, however it has been shown that oocyte and embryo are very susceptible to deleterious effects of high temperature. The cellular damage induced by high temperature could be manipulated by insulin-like growth factor I (IGF-I), which besides minimizing the spontaneous cell death, IGF-I rescue cells which have already initiated the apoptosis induced by different agents or stresses. The first step of this project aimed to evaluate dose-response effect of IGF-I during the maturation in vitro (MIV) in a non-defined medium (pre-experiment 1: MIV - 10% of bovine fetal serum) and semi-defined (preexperiment 2: MIV - 6 mg/mL of bovine albumin serum) in competence of bovine oocytes. Therefore, cumulus-oocyte complexes (CCOs) obtained of cows (Bos indicus crossbreed) from a slaughterhouse were matured in vitro in non-defined and semidefined MIV medium supplemented with 0 (vehicle control), 6,25; 12,5; 25; 50; 100 or 200 ng/mL of IGF-I for 22-24 h. After, oocytes were fertilized (FIV) and cultured in vitro (CIV). The adition of 25 ng/ml of IGF-I on non-defined and semi-defined MIV medium increased porcentage of cleavage on day 3 and blastocysts rate in day 8 after fertilization. In this way, 25 ng/ml IGF-I was used on second step of this experiment, which objective was to evaluate the role of IGF-I on oocyte competence of oocytes collected from Bos indicus - Nelore (NEL; n= 6) and Bos taurus-Holstein (HPB; n= 6) exposed to heat stress. Therefore, cows adapted ...
Mestre
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Loureiro, Paulo Eduardo Fonseca. "Efeito do fotoperíodo na detecção do estro em fêmeas bovinas Nelore (Bos taurus indicus) e cruzadas Red Angus x Nelore e Limousin x Nelore (Bos taurus taurus x Bos taurus indicus) manejadas em diferentes regiões do Brasil." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/74/74131/tde-18102006-153309/.
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A técnica de Inseminação Artificial (IA) está em franco crescimento em rebanhos de bovinos de corte em todas as regiões do Brasil e é a mais utilizada para obter indivíduos cruzados (Bos t. taurus x Bos t. indicus) pela facilidade de implantação nos grandes rebanhos de fêmeas em idade reprodutiva, aliada ao problema da baixa produção de touros taurinos adaptados ao nosso clima. Um dos grandes entraves para o sucesso em um programa de inseminação é a eficiência na detecção do estro. As dificuldades encontradas em outros países têm sido dirimidas através do estudo comportamental das matrizes. A pecuária de corte está espalhada por todo o território brasileiro, mas possui maiores concentrações nas regiões Sudeste, Centro-Oeste e Norte, com importantes diferenças climáticas, como a variação na duração dos dias (fotoperíodo), a sazonalidade pluviométrica, e a própria temperatura do ar, que podem alterar o comportamento sexual das matrizes. Neste sentido o presente trabalho utilizou a monitoração dos períodos de observação de estro em quatro fazendas localizadas no Norte e no Centro Oeste do Brasil, que fazem o uso da técnica da inseminação artificial, para compreender o efeito do fotoperíodo sobre o estro. Foram detectadas 4202 ocorrências de estro em 3358 fêmeas Nelore, 662 fêmeas cruzadas Limousin x Nelore, e 182 fêmeas cruzadas Red Angus x Nelore, submetidas ao mesmo manejo de detecção. A duração média do dia foi de 730,3, 750,8, 771,0 e 795,6 minutos, respectivamente nas fazendas 1 (Pará), 2 (Tocantins), 3 (Goiás) e 4 (Mato Grosso do Sul). Quanto maior a duração do dia mais estros foram detectados pela manhã. Houve diferenças entre as categorias de fêmeas quanto à ocorrência de estro, ficando concentradas as ocorrências de estro das novilhas no período matinal em todas as fazendas e em todos os grupos genéticos. Não houve diferença estatística entre os grupos genéticos dentro das categorias. As vacas solteiras comportaram-se de forma similar as novilhas não havendo diferenças estatísticas na detecção do estro. As vacas paridas tenderam de forma geral a manifestar estro no período da tarde.The artificial insemination (IA) technique is growing in beef cattle herds from all regions of Brazil, especially to obtain Bos t. taurus x Bos t. indicus crossbreds in large herds were the European bulls are not adapted. One of the difficulties of the IA program is the low efficiency of heat detection. In other countries this was avoided through studies of the sexual behavior of the cows. Brazilian beef cattle herds are distributed in all over the territory, but are concentrated mainly in the Southeast, Middle West and North regions, with large climatic differences and day duration (photoperiod), rain seasonality and temperatures that alter the sexual behavior of the cows and heifers. In this way this study used the heat observation in four farms in the North and Middle West regions to study the photoperiod effect on heat detection. A total of 4202 heats were detected in 3358 Nellore, 662 crossbred Limousin x Nellore and 182 crossbred Red Angus x Nellore females, managed under the same detection procedure. The mean daylight duration was 730,3, 750,8, 771,0 and 795,6 minutes, respectively in the farm 1 (Para State), 2 (Tocantins State), 3 (Goias State) and 4 (Mato Grosso do Sul State). As the daylight increased more estrus were detected in the morning. There were differences in cattle categories in estrus detection, with the heifers heat detected in the morning in all farms and in all genetic groups. There was no significant difference between genetic groups inside categories. Cows without calves behaved in a similar way to the heifers and showed no significant difference in heat detection. Cows with calves had a tendency to show estrus in the afternoon.
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Silva, João Carlos Cardoso da [UNESP]. "Fatores que influenciam a produção de embriões de vacas Nelore (Bos taurus indicus) superovuladas." Universidade Estadual Paulista (UNESP), 2002. http://hdl.handle.net/11449/98210.
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silva_jcc_me_jabo.pdf: 166737 bytes, checksum: 76b0116551aaba2c16119c8f6c46924d (MD5)Fatores como idade, dose e tipo de hormônio utilizado na superovulação, início do tratamento de superovulação, uso de progestágeno, estação do ano, propriedade e superovulação repetida foram relacionados com a produção de embriões de vacas Nelore. Os dados analisados envolveram 884 tentativas de superovulação em 318 doadoras com idade entre dois e 21 anos, manejadas em seis propriedades localizadas no Estado de Mato Grosso do Sul. Os dados foram analisados utilizando o GLM do SAS. Dos fatores analisados, somente a idade das doadoras e a propriedade afetaram a produção e a qualidade dos embriões. Vacas senis (>14 anos) produziram, em média, 5,0 l 0,2 e 3,0 l 0,1 embriões (total e viáveis, respectivamente) a menos que vacas jovens (p<0,001), enquanto que um manejo diferenciado aumentou em aproximadamente 2,0 l 0,4 o número de embriões viáveis. O tipo de hormônio (Plusetä, Super-Ovä, Ovagenä, FSH-Pä) ou dosagem (50, 75 ou 100%) utilizada na superovulação não influenciou a produção de embriões (p>0,05). Não houve diferença da resposta quando o tratamento de superovulação foi iniciado entre os dias 8 a 12 do ciclo (p>0,05). A superovulação associada a um implante com progestágenos, iniciado a qualquer momento do ciclo, foi tão eficiente (p>0,05) quanto o tratamento convencional iniciado no meio do ciclo. Finalmente, constatou-se que a superovulação repetida por 6 ou mais vez não afetou a produção ou qualidade dos embriões. Concluiu-se que o manejo e, a idade da doadora são fatores que devem ser considerados ao implementar um programa de transferência de embriões na raça Nelore, por limitar a produção de embriões nessa sub-espécie.
Factors such as age, dose and kind of superovulatory hormone, starting point of the superovulatory treatment, use of progestagens, season, property and repetition of superovulatory treatments were related to embryo production in Nelore cows. Analysed data comprised 884 superovulation attempts on 318 donor cows, with their ages ranging between two and 21 years, managed by six different property owners, in Mato Grosso do Sul State, Brazil. Data were analysed using the General Linear Model of SAS. Among all the studied factors, only the age of donor cows and the property affected embryo production and quality. Older cows (>14 years old) produced a mean of five and three embryos (total and viable, respectively) less than younger ones (p < 0,001), and a better management (property) was responsible for a mean of two more viable embryos. The kind of superovulatory hormone (PlusetTM, Super-OvTM, OvagenTM, FSH-PTM) and its dose (50, 75 or 100%), did not affect embryo production (p > 0,05). Superovulatory response wasnþt different when treatments started on day 8, 9, 10, 11 or 12 of the cycle (p > 0,05), nor when it began with a progestagen implant at any moment of the cycle, as compared to conventional treatments, beginning at the middle of the cycle (p > 0,05). Finally, it was stated that repetition of six or more superovulatory treatments, didn't affect embryo production or quality. It is concluded that management, and specially age of the donors are factors to be taken into account when an embryo transfer program is to be performed in Nelore females, because they affect superovulatory performance in this breed of cows.
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Silva, João Carlos Cardoso da. "Fatores que influenciam a produção de embriões de vacas Nelore (Bos taurus indicus) superovuladas /." Jaboticabal : [s.n.], 2002. http://hdl.handle.net/11449/98210.
Full textAbstract:
Orientador: Rafael Herrera AlvarezBanca: Cesar Roberto Esper
Banca: Pietro Sampaio Baruselli
Resumo: Fatores como idade, dose e tipo de hormônio utilizado na superovulação, início do tratamento de superovulação, uso de progestágeno, estação do ano, propriedade e superovulação repetida foram relacionados com a produção de embriões de vacas Nelore. Os dados analisados envolveram 884 tentativas de superovulação em 318 doadoras com idade entre dois e 21 anos, manejadas em seis propriedades localizadas no Estado de Mato Grosso do Sul. Os dados foram analisados utilizando o GLM do SAS. Dos fatores analisados, somente a idade das doadoras e a propriedade afetaram a produção e a qualidade dos embriões. Vacas senis (>14 anos) produziram, em média, 5,0 l 0,2 e 3,0 l 0,1 embriões (total e viáveis, respectivamente) a menos que vacas jovens (p<0,001), enquanto que um manejo diferenciado aumentou em aproximadamente 2,0 l 0,4 o número de embriões viáveis. O tipo de hormônio (Plusetä, Super-Ovä, Ovagenä, FSH-Pä) ou dosagem (50, 75 ou 100%) utilizada na superovulação não influenciou a produção de embriões (p>0,05). Não houve diferença da resposta quando o tratamento de superovulação foi iniciado entre os dias 8 a 12 do ciclo (p>0,05). A superovulação associada a um implante com progestágenos, iniciado a qualquer momento do ciclo, foi tão eficiente (p>0,05) quanto o tratamento convencional iniciado no meio do ciclo. Finalmente, constatou-se que a superovulação repetida por 6 ou mais vez não afetou a produção ou qualidade dos embriões. Concluiu-se que o manejo e, a idade da doadora são fatores que devem ser considerados ao implementar um programa de transferência de embriões na raça Nelore, por limitar a produção de embriões nessa sub-espécie.
Abstract: Factors such as age, dose and kind of superovulatory hormone, starting point of the superovulatory treatment, use of progestagens, season, property and repetition of superovulatory treatments were related to embryo production in Nelore cows. Analysed data comprised 884 superovulation attempts on 318 donor cows, with their ages ranging between two and 21 years, managed by six different property owners, in Mato Grosso do Sul State, Brazil. Data were analysed using the General Linear Model of SAS. Among all the studied factors, only the age of donor cows and the property affected embryo production and quality. Older cows (>14 years old) produced a mean of five and three embryos (total and viable, respectively) less than younger ones (p < 0,001), and a better management (property) was responsible for a mean of two more viable embryos. The kind of superovulatory hormone (PlusetTM, Super-OvTM, OvagenTM, FSH-PTM) and its dose (50, 75 or 100%), did not affect embryo production (p > 0,05). Superovulatory response wasnþt different when treatments started on day 8, 9, 10, 11 or 12 of the cycle (p > 0,05), nor when it began with a progestagen implant at any moment of the cycle, as compared to conventional treatments, beginning at the middle of the cycle (p > 0,05). Finally, it was stated that repetition of six or more superovulatory treatments, didn't affect embryo production or quality. It is concluded that management, and specially age of the donors are factors to be taken into account when an embryo transfer program is to be performed in Nelore females, because they affect superovulatory performance in this breed of cows.
Mestre
Books on the topic "Bos Reproduction"
1
Mukasa-Mugerwa, E. A review of reproductive performance of female Bos indicus (zebu) cattle. Addis Ababa, Ethiopia: International Livestock Centre for Africa, 1989.
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2
Moya, Jorge Mario Bolanos. Post-partum reproductive performance of Zebu (Bos indicus) cows in the humid tropics. Uppsala: Sveriges Lantbruksuniversitet, 1997.
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3
The boa constrictor, its captive husbandry and reproduction: A comprehensive guide to the best use of specific equipment and techniques to achieve husbandry and reproductive success by Jon Coote. Nottingham: Practical Python, 1993.
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4
Captive husbandry and propagation of the boa constrictors and related boas. Malabar, Fla: Krieger Publishing, 1997.
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Reproduktion: Techniken und Ideen von der Antike bis heute : eine Einführung. Berlin: Dietrich Reimer Verlag, 2011.
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A, Ross Richard. The reproductive husbandry of pythons and boas. Stanford, Calif. (P.O. Box 2227, Stanford 94305): Institute for Herpetological Research, 1990.
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7
Giuliana, Calcani, and Abdulkarim M, eds. Apollodoro e la Colonna Traiana a Damasco: Dalla tradizione al progetto. Roma: "L'Erma" di Bretschneider, 2003.
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8
Pazzini, Karl-Josef. Bilder und Bildung: Vom Bild zum Abbild bis zum Wiederauftauchen der Bilder. Münster: Lit, 1992.
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9
Irle, Klaus. Der Ruhm der Bienen: Das Nachahmungsprinzip der italienischen Malerei von Raffael bis Rubens. Münster: Waxmann, 1997.
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Bios und Zoë: Die menschliche Natur im Zeitalter ihrer technischen Reproduzierbarkeit. Frankfurt am Main: Suhrkamp, 2009.
Find full textBook chapters on the topic "Bos Reproduction"
1
Sartori, Roberto, Jéssica Nora Drum, and Alexandre Barbieri Prata. "Fertility and Infertility in Bos indicus." In Veterinary Reproduction and Obstetrics, 500–509. Elsevier, 2019. http://dx.doi.org/10.1016/b978-0-7020-7233-8.00028-8.
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"Section L - Reproduction." In BIOS Instant Notes in Human Physiology, 390–93. Taylor & Francis, 2013. http://dx.doi.org/10.4324/9780203002650-80.
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"Section L - Reproduction." In BIOS Instant Notes in Human Physiology, 396–418. Taylor & Francis, 2013. http://dx.doi.org/10.4324/9780203002650-82.
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Harris, Donal. "Printing the Color Line in The Crisis." In On Company Time. Columbia University Press, 2016. http://dx.doi.org/10.7312/columbia/9780231177726.003.0003.
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W.E.B. Du Bois’ and Jessie Fauset’s interest in new printing technologies – multigraphs, linotypes, and halftone reproduction – are crucial for understanding the political and aesthetic missions of The Crisis. They envision the magazine as both constituting and creating an archive of a national African American intellectual community; at the same time Du Bois' essays, Fauset's fiction, and Frank Walt's illustrations thematize the technological and political roadblocks for establishing a recognizably black print culture in the 1910s and 1920s.
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"Foreword by Bob Edwards." In Good Clinical Practice in Assisted Reproduction, xi—xiv. Cambridge University Press, 2004. http://dx.doi.org/10.1017/cbo9780511550768.001.
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"Chapter Five The Sexual Politics of Black Internationalism: W. E. B. Du Bois and the Reproduction of Racial Globality." In Wayward Reproductions, 187–226. Duke University Press, 2020. http://dx.doi.org/10.1515/9780822385820-007.
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"Section D – Reproductive anatomy." In BIOS Instant Notes in Plant Biology, 53–66. Garland Science, 2001. http://dx.doi.org/10.1201/9780429258312-10.
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"Section L – Reproductive ecology." In BIOS Instant Notes in Plant Biology, 195–212. Garland Science, 2001. http://dx.doi.org/10.1201/9780429258312-18.
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Jarrett, Michael. "Rolling Tape." In Pressed for All Time. University of North Carolina Press, 2016. http://dx.doi.org/10.5149/northcarolina/9781469630588.003.0003.
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The A&R man became a record producer with the development of magnetic tape (a spoil of World War II) and the introduction of the vinyl long-playing record by Columbia Records in 1948. Producers could capture on tape—for reproduction and sale on records—jazz that had routinely happened for many years only on various stages. When recording technology caught up with the actual practice of improvising musicians, jazz discovered an ideal form in the "album." George Avakian's visionary work with Duke Ellington, Miles Davis, Erroll Garner, Buck Clayton, and Dave Brubeck realized what could be done with the new format and technology. The productions of Milt Gabler, Bob Weinstock, Esmond Edwards, Don Schlitten Teo Macero, Bob Thiele, Orrin Keepnews, Nesuhi Ertegun, Creed Taylor, Lester Koenig, Nat Hentoff ushered in a golden age for jazz.
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"L2 OVERVIEWOF THE FEMALE REPRODUCTIVE SYSTEM." In BIOS Instant Notes in Human Physiology, 394–95. Taylor & Francis, 2013. http://dx.doi.org/10.4324/9780203002650-81.
Full textConference papers on the topic "Bos Reproduction"
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Ponomarjova, Olga, Ilga Sematovica, Inga Piginka-Vjaceslavova, and Aida Vanaga. "Cattle (Bos Taurus) endometrium morphology on the seventh day of the estrous cycle." In Research for Rural Development 2020. Latvia University of Life Sciences and Technologies, 2020. http://dx.doi.org/10.22616/rrd.26.2020.021.
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The aim of our study was to describe the histopathological and cytological characteristic of the cow endometrium on the seventh day of the estrous cycle. In this study, 11 different breeds’ dairy cows (78.18 ± 37.46 months old, in 3.6 ± 2.17 lactation, the mean body condition score 3.4 ± 0.72 (5 points scale)) from Research and Study farm ‘Vecauce’ were selected. All cows were more than 210 days postpartum. Overall health and reproductive tract examination was performed, progesterone (P4) and estradiol (E2) concentration in blood serum were established and the biopsy and cytology samples of endometrium were taken. Mean E2 concentration was 14.92 ± 7.92 pg mL-1, mean P4 concentration was 13.64 ± 9.44 nmol L-1. The mean percentage in the cytology slides was established: epithelial cells 89 ± 9%, polimorphonuclear leukocytes (PMN) 6 ± 5%. Cytological subclinical endometritis (SE) was confirmed in 5 cows. Histopathological findings (out of 22 samples): endometrium stromal edema in 14, hemosiderin and hemosiderophages in 8, supranuclear vacuolization in 12, pseudodecidual reaction in 12 samples. No subnuclear vacuolization and mitosis in the glandular epithelium were detected. Histopathological examination did not reveal SE. Morphology between the uterine horns with and without corpus luteum (CL) and between cows with serum P4 level higher than 15 nmol L-1 and lower than 15 nmol L-1 were not statistically different (p>0.05). In conclusion, histopatological examination is more reliable diagnostic method for SE. Future investigation should be performed to establish cut-off values for the diagnosis of SE in cows more than 210 days postpartum.
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Haefliger, Juerg, R. Stein, Heinrich Walt, and Peter F. Niederer. "High-resolution multispectral imaging system for improved color acquisition and reproduction." In BiOS 2000 The International Symposium on Biomedical Optics, edited by Gregory H. Bearman, Dario Cabib, and Richard M. Levenson. SPIE, 2000. http://dx.doi.org/10.1117/12.379597.
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Glassman, Wenling S., Qin-Ping Liao, Shao-Qing Shi, Linda Goodrum, Gayle Olson, Elizabeth Martin, George Saade, and Robert E. Garfield. "Fluorescence probe for cervical examination during various reproductive states." In BiOS '97, Part of Photonics West, edited by Richard B. Thompson. SPIE, 1997. http://dx.doi.org/10.1117/12.273513.
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Burton, Jason C., Shang Wang, Richard R. Behringer, and Irina V. Larina. "Three-dimensional imaging of the developing mouse female reproductive organs with optical coherence tomography." In SPIE BiOS, edited by Andrew M. Rollins, Scott E. Fraser, and Michael A. Choma. SPIE, 2016. http://dx.doi.org/10.1117/12.2213202.
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Trottmann, M., C. Homann, R. Leeb, D. Doering, J. Kuznetsova, S. Reese, C. G. Stief, S. Koelle, and R. Sroka. "Investigation of the potential of optical coherence tomography (OCT) as a non-invasive diagnostic tool in reproductive medicine." In SPIE BiOS, edited by Bernard Choi, Nikiforos Kollias, Haishan Zeng, Hyun Wook Kang, Brian J. F. Wong, Justus F. Ilgner, Alfred Nuttal, et al. SPIE, 2015. http://dx.doi.org/10.1117/12.2077688.
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Rohmayanti and Reni Mareta. "An Analysis of Early Marriage Determinant and Its Impact on Women’s Reproduction Health." In 1st Borobudur International Symposium on Humanities, Economics and Social Sciences (BIS-HESS 2019). Paris, France: Atlantis Press, 2020. http://dx.doi.org/10.2991/assehr.k.200529.112.
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Indriyani, Diyan. "Analysis of Educational Efforts Towards Teachers’ Perception on Adolescents’ Reproductive Health and Its Problems as an Optimization of Competencies in Mentoring Teenage Girls at School." In 1st Borobudur International Symposium on Humanities, Economics and Social Sciences (BIS-HESS 2019). Paris, France: Atlantis Press, 2020. http://dx.doi.org/10.2991/assehr.k.200529.079.
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Ding, Wei, and Xinyue Yang. "Field Research of Environment Identity System Based on Corporate Identity System." In 13th International Conference on Applied Human Factors and Ergonomics (AHFE 2022). AHFE International, 2022. http://dx.doi.org/10.54941/ahfe1002253.
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Since the 1980s, CIS has been a methodology for many enterprises to improve their brand image. The full English name of CIS is Corporate Identity System. CIS originated from the United States, then developed and perfected in Japan, and began to rise in China in 1980s. Because CIS developed earlier in Taiwan, it has reference value for the correct introduction of CIS in the mainland.On this basis, through continuous practice, MOMA design team put forward a new analysis of CIS. As one of China's top ten design companies and design innovation demonstration enterprises, MOMA design is committed to providing customers with comprehensive solutions from product prototype definition, concept design, structure design, supply chain integration and brand building. MOMA design in the field of nearly 20 years of groping, has been highly recognized by the industry, and the composition of CIS enterprise identification system has a new interpretation, thinks that CIS should include five subsystems: MIS(Mind Identity System), BIS(Behavior Identity System), VIS(Visual Identity System), PIS (Products Identity) System and Environment Identity System (EIS). CIS has been developing for more than 40 years in China, and countless entrepreneurs, practitioners and scholars have gradually perfected their ideas and continuously incorporated some new ideas. However, relatively few literatures can be retrieved in the research of EI. With the advent of sustainable design and digital economy, MOMA design in the long-term project practice that "environment" for the development of corporate image is a state of crisis and opportunity, to a certain extent, has played a key role, and the enterprise's demand for external environment is also growing. In this paper, EI of CIS five elements is taken as the research object and the concept of "field" is adopted. "Field" is derived from Bourdieu's field theory. Field refers to "network or configuration of objective relations between positions". Field, capital and habitus constitute the core of Bourdieu's sociological theory, which embodies the characteristics of relational thinking. Capital is the quantity and type controlled by actors, including economic capital, cultural capital, social capital and symbolic capital. Habitus is an actor's temperament of perception, judgment and action according to different fields. Bourdieu believed that each field should explore the special practical experience of the local nature, and be used as a general field theoretical analysis method, as the construction principle and reproduction mechanism of field practical space. Therefore, relevant scholars extend "CIS field" and "CIS field effect", considering the transverse field mutual relations among the five elements of CIS. This paper takes EI as the sub-field of CIS, considers the mining of EI vertical field to improve the overall integrity of CIS, uses field theory to analyze the macro field, meso field and micro field in environmental identification, subdivides the environment contained in each field, and sort out the overall logical framework of EIS. Then through the case of MOMA design, using capital and habitus as media to verify the cross relations between the three dimensions of the segmentation of environmental identity system. This paper aims to further improve CIS and put forward the importance of EI, hoping to promote the collaborative evolution of enterprises themselves, enterprises with enterprises and enterprises with the outside world in this field, and also hope to bring certain reference value to some practitioners and academic staff.
Reports on the topic "Bos Reproduction"
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Yuen, Hudson. Reproduction of 'Inside the Box: Safety, Health, and Isolation in Prison'. Social Science Reproduction Platform, February 2022. http://dx.doi.org/10.48152/ssrp-qyrc-cn64.
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Long, James M., Trevor A. Starks, Tyler Farling, and Robert Bastarache. Assessing the potential for rainbow trout reproduction in tributaries of the Mountain Fork River below Broken Bow Dam, southeastern Oklahoma. Asheville, NC: U.S. Department of Agriculture, Forest Service, Southern Research Station, 2016. http://dx.doi.org/10.2737/srs-rp-58.
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Long, James M., Trevor A. Starks, Tyler Farling, and Robert Bastarache. Assessing the potential for rainbow trout reproduction in tributaries of the Mountain Fork River below Broken Bow Dam, southeastern Oklahoma. Asheville, NC: U.S. Department of Agriculture, Forest Service, Southern Research Station, 2016. http://dx.doi.org/10.2737/srs-rp-58.
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Salamanca-Carreño, Arcesio, Jannet Bentez Molano, and René Alejandro Crosby. Aspectos generales del sistema de producción ganadera doble propósito en el municipio de Arauca (Colombia). Ediciones Universidad Cooperativa de Colombia, November 2021. http://dx.doi.org/10.16925/wpri.1.
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Las explotaciones del sistema ganadero doble propósito están constituidas por animales mestizos en diferentes proporciones Bos taurus-Bos indicus; su principal característica es el ordeño con presencia del ternero. Otro aspecto para tener en cuenta es que, en la mayoría de las explotaciones no se cuenta con información y, si existe, no es analizada ni interpretada. El documento tiene como objetivo presentar información de la funcionalidad técnica y estructural del sistema doble propósito en Arauca. Los datos fueron recolectados durante el desarrollo de un proyecto de investigación; se realizó una descripción de las características generales del sistema, su estructuración, aspectos de manejo y alimentación, así como los indicadores productivos y reproductivos, expresados en producción de carne y leche de los grupos raciales. Este es el primer manuscrito que reporta información de tipo productiva de los grupos raciales existentes. Se concluye que el manejo del sistema ganadero doble propósito, aún tradicional, y su mejoramiento genético para aumentar la producción consiste en la introducción de “razas de moda”, por lo que se olvida el efecto de los factores medio ambientales. La falta de registros se asocia al desconocimiento de la productividad y de la estructura, así como la funcionalidad del sistema doble propósito.
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Zeidenstein, Sondra, and Kirsten Moore. Learning About Sexuality: A Practical Beginning. Population Council, 1996. http://dx.doi.org/10.31899/pgy1996.1007.
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“Learning About Sexuality: A Practical Beginning” is divided into three main parts. The first includes approaches that program staff, activists, and researchers are taking to understand people’s experiences of sexuality. The second explores the explicit and implicit links among health-seeking behavior, contraceptive practice, reproductive health, and sexuality. The chapters in part three focus on activities that challenge entrenched attitudes and behavior about sexuality that have real and potentially harmful effects on women’s and men’s reproductive health. The book features program and research work in all regions of the world with women, men, girls, and boys. The chapters are written by authors from over a dozen countries, with over half the contributions coming from developing countries. Collectively, these chapters represent an exploration of the relationship of sexuality to reproductive health, contraceptive practice, and overall well-being. For all their variety of place, approach, and focus, a number of common themes emerge.
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Graft, Auralice, Nicole Haberland, and Rachel E. Goldberg. Married adolescents: A review of programmes. Population Council, 2004. http://dx.doi.org/10.31899/pgy22.1006.
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Historically, major adolescent and reproductive health initiatives have failed to explicitly consider the needs of married adolescents. This paper provides insight into what is being done—or not being done—to support married adolescent girls and boys, how these populations’ needs are being conceptualized, and the extent to which social context is factored into program design. Some early work with adolescent mothers (married and unmarried) is considered. The degree to which selected adolescent programs have been able to reach married girls with their activities is briefly examined. A few basic parameters of potential interventions for married adolescents are presented, including an inventory of current projects, to examine how, when, and at whom efforts typically are directed. Finally, three in-depth examples of recent, ongoing programs for married adolescent girls are presented.
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Reproduction of 'Privatization and business groups: Evidence from the Chicago Boys in Chile'. Social Science Reproduction Platform, December 2021. http://dx.doi.org/10.48152/ssrp-hydw-7a71.
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Bedrock geologic map of the Promontory Mountains, Box Elder County, Utah (GIS reproduction of USGS OF 88-646 [1988]). Utah Geological Survey, 2019. http://dx.doi.org/10.34191/ofr-698dr.
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India: Enhancing girls' life skills requires long-term commitment. Population Council, 2005. http://dx.doi.org/10.31899/rh16.1003.
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While adolescents in India face a rapidly changing economic environment, the choices available to unmarried girls are very different from those available to boys. Girls are much less likely than boys to remain unmarried into their twenties, complete middle school, or generate income. Due to social norms, they have limited control over their life choices, and are less likely than boys to be allowed mobility within or beyond their immediate community. In 2001, the Population Council teamed with CARE India to test a pilot intervention to enhance skills and expand life choices for adolescent girls living in the slums of Allahabad. The 10-month intervention tested the effect of the skills intervention on the girls’ reproductive health knowledge, social contacts and mobility, self-esteem, and perception of gender roles. The impacts were assessed using survey responses from girls who were interviewed in both baseline and endline surveys. As noted in this brief, girls and their parents found the life skills training acceptable, but the intervention had little overall impact.
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GIRL Center Roadmap to 2030. Population Council, 2022. http://dx.doi.org/10.31899/sbsr2022.1031.
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Today’s rising generation of 1.3 billion adolescents are tomorrow’s future. Yet, adolescent girls fare worse than boys in many ways, including education and experiences with violence. Furthermore, the implications of key experiences for girls in adolescence—school dropout, early marriage, motherhood, sexual and gender-based violence—are irreversible. The GIRL Center Roadmap to 2030 outlines the strategy of the Population Council’s GIRL Center—generating and communicating evidence, convening experts, and nurturing talent. Undertaking a girl-centered, evidence-driven, intersectional, and innovative approach that is grounded in low- and middle-income countries, the GIRL Center focuses on: intergenerational poverty and gender inequity; adolescents and the digital space; adolescents in humanitarian settings; adolescent sexual and reproductive health; adolescent economic empowerment; and adolescents and climate. These focus areas aim to advance the GIRL Center’s vision of a gender-equitable world where girls and boys make a healthy and safe transition into adulthood and reach their full potential.