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Dissertations / Theses on the topic 'Bombyx mori silkworm'

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Published: 4 June 2021

Last updated: 10 February 2022

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1

Åberg, Gabriel. "Intrinsically Functionalized Silk (Bombyx Mori)." Thesis, Linköpings universitet, Institutionen för fysik, kemi och biologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-97636.

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The goal of the thesis is to incorporate materials with either fluorescent or conductive properties in silk fibers, by feeding silkworms with a diet containing these materials. To achieve this, one would have to breed (rear) silkworm from eggs into larvaes, then to feed the silkworms with this special diet containing fluorescent or conductive materials. Samples of silk were then collected either from spun cocoons or via removing the silk producing organs (silk glands) from the silkworms via dissection. The samples were then analyzed with absorbance spectrometer, spectrofluorometer or via photoluminesecent measurement to determine if any materials had been incorporated into the silk fibers. Silkworms were successfully reared from eggs up to moths, once the silkworm larvae had grown enough in size their diet were switches from their regular food (silkworm chow) to food containing conjugated molecules or polymers with fluorescent or conductive properties. A total of 14 materials were tested. One material gave a clear positive result and that was from the fluorescent compound Rhodamine B. Other fluorescent materials, Nile red and POWT yielded some results indicating their presence in the silk but the results were not conclusive. The rest of the materials all failed with being incorporated within the silk fibers; this was due to their lethality, size, lack of zwitterionic properties and such. The properties of the materials are of great importance for the uptake process, where a small zwitterionic molecule has a great change of being taken up and incorporated in the silk fibers. Whereas a big materials such as a polymer without any zwitterionic will in most cases just follow through the food in the digestive track without any uptake.

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2

Trancik, Jessika. "Silk microstructures." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249173.

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3

Blossman-Myer, Bonnie. "A Contravention of Established Principles of Interspecific Allometric Metabolic Scaling in Developing Silkworms, Bombyx Mori." Thesis, University of North Texas, 2007. https://digital.library.unt.edu/ark:/67531/metadc3704/.

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Established interspecific metabolic allometric relationships do not adequately describe the complexity and variable physiological states of developing animals. Consequently, intraspecific allometric relationships of oxygen consumption and carbon dioxide production as a function of body mass; the respiratory quotient; the function of the silk cocoon; and body composition were investigated for each distinct developmental stage of the silkworm, Bombyx mori. Whole animal O2 consumption in Bombyx ranged from 0.00064 + 0.000047 ml O2 .hr-1 at larval instar I to 0.77 + 0.06 ml O2 .hr-1 in pre-pupal, falling to 0.21+ 0.01 ml O2 .hr-1 in the pupae. Those instars having a significant relationship between O2 consumption as a function of body mass, the slope of the line relating O2 consumption to body mass varied between 0.99 and 1.02, while across all instars the slope was 0.82. Developmental allometry should be presented for individual developmental stages because the individual allometric exponents of the stages can be significantly different from the overall allometric exponent throughout development and in some cases, the overall allometric exponent can be a statistical artifact. The first larval instar of Bombyx mori has the lowest cross sectional area of high metabolic tissue of the midgut (27%) and had one of the highest percentages of some metabolically inert tissues (i.e. lipid, 7.5%). Body composition of the first instar does not support the idea that smaller mass animals having the highest O2 consumption are composed of a greater percentage of metabolically active organs when compared to larger animals. However, this developmental stage has the highest percentage of the mitochondrial marker cytochrome oxidase, which correlates well with the high O2 consumption rate of the smaller mass. Therefore, established interspecific principles should not be assumed to function as valid models for intraspecific developmental relationships of metabolism as a function of body mass. Developmental allometry should include an analysis of individual stages of development as well as an analysis of development as a whole to gain a comprehensive understanding of the complexity of allometry of the developing animal such as the silkworm.

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4

Begum, Hosne Ara. "Hybridization of mulberry silkworm (Bombyx Mori L.) for higher silk productivity and disease resistance /." [St. Lucia, Qld.], 2006. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe19789.pdf.

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5

Santorum, Marilucia. "Avaliação da toxicidade do inseticida novaluron em Bombyx mori (Lepidoptera Bombycidae) /." Botucatu, 2019. http://hdl.handle.net/11449/182490.

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Orientador: Daniela Carvalho dos Santos
Resumo: O bicho-da-seda, Bombyx mori (Lepidoptera: Bombycidae), é o inseto de maior importância econômica na produção de seda. A lagarta se alimenta de folhas de amoreira e é altamente sensível a agrotóxicos, assim o uso destes em culturas agrícolas circunvizinhas às plantações de amoreira pode afetar o desenvolvimento de B. mori, acarretando em desequilíbrio nas suas funções metabólicas e, consequentemente, comprometendo a produção de casulos. Entre estes agrotóxicos, destaca-se o Novaluron, inseticida inibidor da síntese de quitina nos insetos e empregado no controle de insetos pragas de culturas agrícolas próximas as plantações de amoreira. Assim, investigamos os efeitos tóxicos de Novaluron no desenvolvimento de B. mori. Lagartas de B. mori, foram separadas em dois grupos experimentais: grupo controle (GC) e grupo tratamento (GT: tratado com 0, 15 mL/L de Novaluron). Após ecdise do 2° para o 3° instar, lagartas do GT foram alimentadas por 24 horas com folhas de amoreira tratadas com o inseticida. Paralelamente foi realizada uma nova exposição, porém em lagartas que realizavam a ecdise do 4° para o 5° instar. Lagartas, pupas e adultos de B. mori foram anestesiadas e segmentos do intestino médio, glândula da seda e órgãos reprodutores retirados e processados convencionalmente para técnicas de microscopias de luz, eletrônica e imunohistoquímica. Além disso, os efeitos no desenvolvimento, reprodução e qualidade do casulo também foram avaliados. O Novaluron provocou efeitos citotóxico... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The silkworm, Bombyx mori (Lepidoptera: Bombycidae is the insect of major economic importance in the production of silk. The larvae feeds on mulberry leaves and is highly sensitive to agrochemicals, thus the use of these in agricultural crops surrounding the mulberry plantations can affect the development of B. mori, causing an imbalance in its metabolic functions and, consequently, compromising the production of cocoons. Among these agrochemicals, stands out the Novaluron, an insecticide inhibitor of the synthesis of chitin in insects and used in the control of insect pests of crops near mulberry plantations. Thus, we investigated the lethal and sublethal effects of Novaluron on the development of B. mori. Larvae were selected into two experimental groups: control group (CG) and treatment group (TG: treated with 0, 15 mL/L Novaluron). After ecdysis from the 2nd to the 3rd instar, the TG larvae were fed for 24 hours with mulberry leaves treated with the insecticide. In parallel, a new exposition was carried out, however in larvae that carry out the ecdysis from the 4th to the 5th instar. B. mori larvae, pupae and adults were anesthetized and segments of the midgut, silk gland and reproductive organs were removed and processed conventionally for light microscopy, electron microscopy and immunohistochemistry. In addition, the effects on the development, reproduction and quality of the cocoon were also evaluated. Novaluron caused cytotoxic effects on the midgut and the silk glan... (Complete abstract click electronic access below)
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6

Krasnov, Igor [Verfasser]. "Mechanical properties of Bombyx mori silkworm silk: viscoelasticity, structural and molecular origin / Igor Krasnov." Kiel : Universitätsbibliothek Kiel, 2013. http://d-nb.info/1045604046/34.

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7

Lunke, Martine Dominique. "Molecular characterization of BmGATA-beta, a gene involved in choriogenesis in the silkworm Bombyx mori." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0027/NQ49515.pdf.

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8

Rao, Sudhakara P. "Studies on the evolution of adaptive bivoltine breeds of silkworm bombyx mori L. for tropical climates." Thesis, Studies on the evolution of adaptive bivoltine breeds of silkworm bombyx mori L. for tropical climates, 2003. http://hdl.handle.net/2009/3159.

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9

Diao, Yupu, Anrui Lu, Bing Yang, Wenli Hu, Qing Peng, Qing-Zhi Ling, Brenda T. Beerntsen, Kenneth Söderhäll, and Erjun Ling. "Existence of Prophenoloxidase in Wing Discs : A Source of Plasma Prophenoloxidase in the Silkworm, Bombyx mori." Uppsala universitet, Jämförande fysiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-184482.

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In insects, hemocytes are considered as the only source of plasma prophenoloxidase (PPO). PPO also exists in the hemocytes of the hematopoietic organ that is connected to the wing disc of Bombyx mori. It is unknown whether there are other cells or tissues that can produce PPO and release it into the hemolymph besides circulating hemocytes. In this study, we use the silkworm as a model to explore this possibility. Through tissue staining and biochemical assays, we found that wing discs contain PPO that can be released into the culture medium in vitro. An in situ assay showed that some cells in the cavity of wing discs have PPO1 and PPO2 mRNA. We conclude that the hematopoietic organ may wrongly release hemocytes into wing discs since they are connected through many tubes as repost in previous paper. In wing discs, the infiltrating hemocytes produce and release PPO probably through cell lysis and the PPO is later transported into hemolymph. Therefore, this might be another source of plasma PPO in the silkworm: some infiltrated hemocytes sourced from the hematopoietic organ release PPO via wing discs.

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10

Santorum, Marilucia. "Infecção de células da ampola dos túbulos de MALPIGHI de Bombyx Mori pelo Alphabaculovirus." Universidade Estadual do Oeste do Parana, 2015. http://tede.unioeste.br:8080/tede/handle/tede/650.

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Made available in DSpace on 2017-07-10T14:17:10Z (GMT). No. of bitstreams: 1 AMPOLA DOS _ MALPIGHI DE Bombyx mori PELO ALPHABACULOVIRUS.pdf: 2176663 bytes, checksum: 866566cf54573cb621db0597bbb30516 (MD5) Previous issue date: 2015-02-24
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Bombyx mori nucleopolyhedrovirus (BmNPV) is an entomopathogenic virus Baculoviridae family, Alphabaculovirus genre, which infects the silkworm, Bombyx mori and causes nuclear polyhedrosis disease. A viral geographic isolated from BmNPV was identified in the state of Paraná, Brazil, and a number of organs and target tissues were identified, but there is no information about the infection of Malpighian tubules (MT). MT make up the excretory system of B. mori, acting in the elimination of toxic substances and electrolyte homeostasis. This study aimed to analyze the behavior on the susceptibility and cytopathology of MT B. mori to BmNPV. Therefore, hybrid B.mori caterpillars at 5º instar, were inoculated experimentally with a viral suspension of BmNPV. On different days post-inoculation (dpi), the 4° to 9° dpi, MT segments were collected, divided in regions of the ampullae, proximal, medial and distal; following the processing for light and electron microscopy transmission. Material control followed the same process that the inoculated. The results showed that B. mori MT showed variations in their morphology and susceptibility to BmNPV according to the analyzed region. Thus, the region of the ampullae in its transition area to the proximal, appeared susceptible to BmNPV, from 6° dpi, while other regions showed no evidence of infection by the pathogen. The transition area is a small region which is located at the end of the ampullae and the beginning of the proximal region of MT. The cellular morphology of simple lining epithelium showed continuous variation of pavement, characteristic of the ampullae, the cylindrical, in the transition area; already continuous with the proximal region of MT, this morphological change was abruptly. The cytopathology revealed in hypertrophic nucleus, viroplasm containing the nucleocapsid and viral polyhedra. At the end of the infectious cycle was observed cytolysis of infected cells, releasing polyhedra in the extracellular medium, both in hemocele as in MT lumen, a factor that may favor the transmission of the disease. Thus, infection of the ampullae in its transition area to the proximal region, will contribute to greater scientific knowledge of cytopathology aspects and targets of infection from this virus isolated geographical, for the establishment of its infectious cycle of the insect's body BmNPV
Bombyx mori nucleopolyhedrovirus (BmNPV) é um vírus entomopatogênico da família Baculoviridae, gênero Alphabaculovirus, que infecta o bicho-da-seda, Bombyx mori (Lepidoptera: Bombycidae) e causa a doença poliedrose nuclear. Um isolado geográfico viral do BmNPV foi identificado no estado do Paraná, Brasil, e uma série de órgãos e tecidos alvos foram identificados, porém não há informações sobre a infecção dos túbulos de Malpighi (TM). Os TM compõem o sistema excretor de B. mori, atuando na eliminação de substâncias tóxicas e na homeostase hidroeletrolítica. Assim, este estudo objetivou analisar o comportamento referente à susceptibilidade e citopatologia dos TM de B. mori ao BmNPV. Para tanto, lagartas híbridas de B. mori, 5° instar, foram inoculadas experimentalmente com uma suspensão viral de BmNPV. Em diferentes dias pós-inoculação (dpi), do 4° ao 9° dpi, segmentos dos TM foram coletados, subdivididos nas regiões da ampola, proximal, média e distal; seguindo o processamento para as microscopias de luz e eletrônica de transmissão. Material controle seguiu o mesmo processamento que o inoculado. Os resultados demonstraram que os TM de B. mori, apresentaram variação na sua morfologia e susceptibilidade ao BmNPV de acordo com a região analisada. Assim, a região da ampola, na sua área de transição com a proximal, se apresentou susceptível ao BmNPV, a partir do 6° dpi, enquanto as demais regiões não evidenciaram indícios de infecção pelo patógeno. A área de transição é uma pequena região que se localiza ao término da ampola e inicio da região proximal do TM. A morfologia celular do epitélio de revestimento simples apresentou variação continua de pavimentoso, característico da ampola, a cilíndrico, na sua área de transição; já na continuidade com a região proximal do TM, esta variação morfológica foi abrupta. A citopatologia revelou no núcleo hipertrófico, o viroplasma, contendo os nucleocapsídeos e os poliedros virais. Ao final do ciclo infeccioso foi verificado a citólise de células infectadas, liberando poliedros no meio extracelular, tanto na hemocele como no lúmen do TM, fator que pode favorecer a transmissão da doença. Assim, a infecção da ampola, na sua área de transição com a região proximal, irá contribuir com um maior conhecimento científico dos aspectos citopatológicos e dos alvos de infecção deste isolado geográfico viral, visando o estabelecimento do seu ciclo infeccioso do BmNPV no corpo do inseto.

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11

Giacomin, Alessandra Maria. "Dinâmica da inovação da sericicultura no Brasil." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/100/100133/tde-14102018-180657/.

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O Brasil destaca-se como maior produtor de seda em escala comercial no Ocidente e o quinto maior no mundo, devido ao eficiente sistema integrado de produção, que qualifica os fios de seda brasileiros como de alta qualidade. A sericicultura é a criação do Bombyx mori L. (bicho-da-seda) para obtenção dos casulos de seda para a fiação. A atividade emprega, no Brasil, aproximadamente, 2.500 famílias no meio rural, gerando renda por cerca de 8 a 9 meses do ano, contribuindo para a fixação delas ao campo. Além disso, cerca de 2.000 funcionários trabalham nas empresas de fiação no meio urbano, e o total de empregos, contando com os empregos indiretos, associados à atividade, somam, aproximadamente, 20.000. Esta pesquisa teve como objetivo apresentar a dinâmica da inovação da sericicultura no Brasil e as medidas que vêm sendo tomadas nesse sentido. Os dados, para elaboração deste estudo, foram coletados por meio de análise bibliográfica, assim como, visitas técnicas às empresas e aos produtores ligados à sericicultura nos estados do Paraná e São Paulo. Foram realizadas entrevistas com quatro sericicultores, juntamente com representantes das empresas Fiação de Seda BRATAC S.A., Fiação de Seda Artesanal O Casulo Feliz, Instituto Vale da Seda e com Enéas Neto, estilista residente da marca Vale da Seda. As principais modernizações constatadas visam diminuir o esforço físico do trabalhador rural e aumentar a produtividade, mantendo os padrões de qualidade. Constatou-se, nas visitas e entrevistas aos produtores rurais, que a sericicultura é, na maioria dos casos, a principal fonte de renda familiar, afirmando a importância social e econômica da referida atividade e que, mesmo após anos anteriores de declínio, vem se mostrando promissora no Brasil, com aumentos gradativos nas últimas safras, tornando-se uma atividade bastante rentável. Por meio das entrevistas com os empresários e representantes do setor, relacionados com a produção de seda no Brasil, percebe-se que se sentem otimistas com relação ao futuro da seda, pois existe hoje uma maior preocupação com o uso de produtos provenientes de extração mais sustentável, duráveis e com maior valor agregado e, a seda, se enquadra nesses requisitos. Além disso, estudos preliminares indicam uma correlação positiva entre a produção de seda e a mitigação da Pegada de Carbono. Desse modo, a sericicultura, de forma sustentável, ética e socialmente responsável, poderá apresentar cada vez maior destaque e, por consequência, refletir positivamente na economia brasileira como um todo. Conclui-se que a sericicultura é uma atividade economicamente viável para o Brasil, o qual apresenta condições de solo e clima favoráveis para a criação do bicho-da-seda, em diversas regiões de seu território, com a possibilidade de gerar empregos e renda, tanto na área rural, quanto na urbana. Impulsionada pela mecanização e modernização da atividade agropecuária, a sericicultura nacional vem retomando lugar de destaque
Brazil stands out as the largest commercial scale silk producer in the West and the fifth largest in the world due to its efficient integrated production system, which qualifies Brazilian silk yarns as high quality. The sericulture is the creation of Bombyx mori L. (silkworm) to obtain silk cocoons for spinning. The activity employs approximately 2,500 rural families in Brazil, generating income for about 8 to 9 months of the year, contributing to their setting in the field. In addition, around 2,000 employees work in the spinning companies and the total amount of jobs, added to the indirect jobs by the activity, is, approximately, 20,000. This research aimed to present the dynamic of sericulture innovation in Brazil and the measures that have been taken in this direction. All the data for the preparation of this study were collected through bibliographic analysis, as well as technical visits to companies and producers linked to sericulture in the states of Parana and Sao Paulo. Interviews were conducted with four sericulturists, along with representatives of the spinning companies of Silk BRATAC S.A., spinning of handmade silk O Casulo Feliz, Silk Valley Institute and with Eneas Neto, resident designer of the Silk Valley brand. The main modernizations observed aim to reduce the physical effort of the rural worker and increase productivity, maintaining the renowned Brazilian quality standards. In the visits and interviews with rural producers, sericulture is, in most cases, the main source of income of the family, affirming the social and economic importance of this activity, and that, even after previous years of decline, showing promising in Brazil, with gradual increases in the last harvests, becoming a very profitable activity for producers of other crops. Through interviews with businessmen and industry representatives related to the production of silk in Brazil, it is perceived that they feel optimistic about the future of silk, as there is a greater concern with the use of products from more sustainable extraction, durable and with higher added value, and silk meets these requirements. Furthermore, preliminary studies indicate a positive correlation between silk production and Carbon Footprint mitigation. In this way, sericulture, in a sustainable, ethical and socially responsible manner, may be increasingly prominent, and consequently, positively reflecting on the Brazilian economy as a whole. It is concluded that sericulture is an economically viable activity for Brazil that presents favorable soil and climate conditions for the creation of silkworms, as well as generates jobs and income in the rural area, contributing to a decrease in the rural exodus. Driven by the mechanization and modernization of agricultural activity, the national sericulture has resumed a prominent place

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Ouyang, Ching. "Investigation of the role of TBP-TATA interaction in differential transcription of two alanine tRNA genes in silkworm Bombyx mori /." view abstract or download file of text, 1999. http://wwwlib.umi.com/cr/uoregon/fullcit?p9947978.

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Thesis (Ph. D.)--University of Oregon, 1999.
Typescript. Includes vita and abstract. Includes bibliographical references (leaves 90-101). Also available for download via the World Wide Web; free to University of Oregon users. Address: http://wwwlib.umi.com/cr/uoregon/fullcit?p9947978.

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Blossman-Myer, Bonnie Burggren Warren W. "A contravention of established principles of interspecific allometric metabolic scaling in developing silkworms, Bombyx mori." [Denton, Tex.] : University of North Texas, 2007. http://digital.library.unt.edu/permalink/meta-dc-3704.

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Martinez, Maria Juanita. "Transcription factor IIIB binding to two classes of Alanine tRNA gene promoters of the silkmoth, Bombyx mori /." view abstract or download file of text, 2001. http://wwwlib.umi.com/cr/uoregon/fullcit?p3018382.

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Thesis (Ph. D.)--University of Oregon, 2001.
Typescript. Includes vita and abstract. Includes bibliographical references (leaves 128-143). Also available for download via the World Wide Web; free to University of Oregon users.

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Davies, Gwilym. "Natural and bioinspired silk spinning." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:e7ec14e5-efff-4e19-b1a0-4c9f02ade678.

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This thesis describes an investigation into silk spinning, with the objective of producing high performance silk fibres in the laboratory using a novel spinning device based upon observations on natural spinning glands and processes. After an in-depth literature review the work is reported in two sections: natural and artificial spinning. The literature provides fragmented data on different aspects of natural silk production, and artificial spinning has not yet reproduced fibres with the properties of native silk fibres, despite unfounded claims of biomimetic spinning. The first half of the thesis looks at natural silk spinning. The work started with a general study of the morphology of spider and silkworm spinning ducts: First, how the silk fibre develops as the dope flows through the gland; and second the relationship between silk fibre properties and both gland morphology and spinning speed. More detailed studies using histochemical and spectroscopic investigations showed that the silk ducts of the spider Nephila edulis and the silkworm Bombyx mori both contain β-chitin, despite an evolutionarily distant common ancestor. Finally, observations showed that the duct of N. edulis consists of alternating nanoporous discs, and FEA modelling indicated that the duct is optimised for mechanical integrity and permeability. The second half of the thesis describes the development of a spinning device that uses natural silk dope mainly taken from B. mori as feedstock. It begins with a description of the gradual development of the engineering aspects of the spinning device, to meet challenges raised during the spinning investigation. The development of a centrifugal capillary rheometer, for practical quantitative insights into rheological processes is then presented. Finally the spinning investigation is reported: first, the screening of spinning in glass capillaries based upon natural gland dimensions and flow rates, which have been shown to induce fibrillation in silk dope in a rheometer, and also included initiation of instability through heat applied along the capillary; second, the final spinning evaluation, using lessons learned from all the screening trials throughout the project, but also including a key development of a hydrophobic coating on the capillary tip to inhibit droplet formation and massively increase the process stability and ease of fibre production. The main conclusions from this work are that good silk fibre cannot be spun by flow shear stress alone; and, that heat instability induces indiscriminate gelation of the silk, whose disordered molecular structure gives poor silk fibre properties. The body of work behind these conclusions provides fundamental background information and new insights that will contribute to the next stages of development of artificial silk spinning, from obtaining a better understanding of the biology of natural spinning glands to the engineering difficulties of implementing the bioinspired principles.

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SAJWAN, Suresh Chandra Singh. "Gene targeting in Silkworm (Bombyx mori) by Engineered Endonucleases." Doctoral thesis, 2013. http://www.nusl.cz/ntk/nusl-151839.

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This thesis describes the establishment of a precise gene targeting methodology in the silkworm Bombyx mori by technologies based on engineered endonucleases. Two classes of engineered endonucleases, ZFNs and full length TALENs were used for creating DSBs at specified sites in the colour marker genes (BmBlos2 and Bmwh3). Direct embryo microinjection of engineered nucleases mRNA were performed and let the nuclease proteins to disrupt the functions of these marker genes by creating DSBs and inducing error prone NHEJ mechanism. These experiments showed that both ZFNs and TALENs could be used for targeted gene disruption in silkworms.

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Palhan, Vikas B. "Bombyx Mori Nuclear Polyhedrosis Virus : Molecular Biology And Biotechnology Applications." Thesis, 1995. http://etd.iisc.ernet.in/handle/2005/1651.

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Swamy, Nanjunda L. "Studies on the combining ability in silkworm Bombyx mori L." Thesis, 1997. http://hdl.handle.net/2009/1471.

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Reddy, Dayananda R. "Biochemical toxicology of dimethoate (organophosphorus pesticide) in silkworm bombyx mori." Thesis, 1994. http://hdl.handle.net/2009/2472.

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Ramesh, C. K. "Development of Hardy bivoltine Races of Silkworm Bombyx Mori L." Thesis, 1996. http://hdl.handle.net/2009/5971.

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Zhang, K., M. Xu, J. Su, Z. Sun, Y. Li, W. Zhang, J. Hou, Lijun Shang, and H. Cui. "Characterization and identification of the integrin family in silkworm, Bombyx mori." 2014. http://hdl.handle.net/10454/10480.

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Yes
As an important economic insect, Bombyx mori is also a useful model organism for lepidopteran insect. Integrins are evolutionarily conserved fromsponges to humans, and play vital roles inmany physiological and pathological processes. To explore their diverse functions of integrins in insect, eleven integrins including sixα and five β subunitswere cloned and characterized fromsilkworm. Our results showed that integrins fromsilkwormown more family members compared to other invertebrates. Among those α subunits, integrins α1, α2, and the other four subunits belong to PS1, PS2, and PS3 groups, respectively. The β subunits mainly gather in the insect βν group except the β1 subunit which belongs to the insect β group. Expression profiles demonstrated that the integrins exhibited distinct patterns, but were mainly expressed in hemocytes. α1 and β2 subunits are the predominant ones either in the embryogenesis or larva stages. Interestingly, integrins were significantly up-regulated after stimulated by 20-hydroxyecdysone (20-E) in vivo. These results indicate that integrins performdiverse functions in hemocytes of silkworm. Overall, our results provide a newinsight into the functional and evolutionary features of integrins.
National Basic Research Programof China (No. 2012cb114603), the Research Fund for the Doctoral Program of Higher Education of China (20130182110003), the Natural Science Foundation of Chongqing (cstc2013jcyjys0007), and the Fundamental Research Funds for the Central Universities (SWU111014).

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22

Maribashetty, V. G. "Evolution of superior bivoltine races of silkworm. Bombyx mori. L for tropics." Thesis, 1991. http://hdl.handle.net/2009/1465.

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23

Rajanna, G. S. "Evolution of suitable bivoltine races of silkworm bombyx mori L. for tropics." Thesis, 1989. http://hdl.handle.net/2009/2840.

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24

Udupa, S. Rajesh. "Proliferating Cell Nuclear Antigen From The Mulberry Silkworm Bombyx mori : Cloning And Characterisation." Thesis, 1999. http://etd.iisc.ernet.in/handle/2005/1566.

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25

Himantharaj, M. T. "Studies on the influence of nutrition on fecundity in silkworm Bombyx Mori L." Thesis, 1994. http://hdl.handle.net/2009/1494.

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26

Kumar, Nirmal S. "Studies on the Synthesis of appropriate Silkworm breeds (Bombyx Mori L ) for tropics." Thesis, 1995. http://hdl.handle.net/2009/2349.

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27

Babu, Srinivas G. K. "Studies on some aspects of Industrial seed production in silkworm Bombyx Mori. L." Thesis, 1993. http://hdl.handle.net/2009/5930.

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28

Sung, Hsu-Min, and 宋旭民. "Functional Regulation of Baculovirus Homologous Region by Cellular microRNAs of Silkworm Bombyx mori." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/64873002393493768538.

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碩士
國立陽明大學
生命科學系暨基因體科學研究所
100
Abstract Individual baculovirus homologous region (hr) contains 2 to 8 tandem repeats of imperfect palindrome sequence and has been proved to function as a transcriptional enhancer in both insect and mammalian cells. Besides, it can function as a replication origin in a virus-dependent manner. In this study, we found that when the microRNA (miRNA) pathway was abolished, the replication of AcMNPV could increase significantly in non-host BmN cells; therefore, possibility exist that certain miRNAs in BmN cells might suppress the functions of hrs and block the replication of the virus. With the aid of in silico prediction, we identified 10 Bombyx mori cellular miRNAs with potential binding sites on hr. Reporter assay and qPCR studies showed that the function of hr as a transcriptional enhancer and replication origin were both significantly suppressed by certain Bombyx mori miRNAs. Those miRNAs can significantly suppress the function of hr were predicted to have high binding scores against the palindrome sequence of hrs. Two of these miRNAs, bmo-2808a and bmo-2808b, were both predicted to have multiple binding sites on the viral DNA (hr1, hr2, hr3, hr4, hr5). It was proven that the binding of IE1 on palindrome sequence is important for hr to display its full function; therefore, it is reasonable for us to propose that bmo-2808a and bmo-2808b might suppress the function of hr via occupying the IE1 binding sites on palindrome sequence. This hypothesis is supported by our observation that the number of IE1 foci in the nucleus is decreased when co-transfected with either bmo-2808a or bmo-2808b, indicating these miRNAs function as inhibitors to keep IE1 away from its binding sites on hr. Although further experiments are still needed, our results may have identified a novel role of miRNA to the functional regulation of DNA replication and / or enhancing of transcription.

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29

Chen, Shue-Fang, and 陳淑芳. "Hormonal Control of DNA Synthesis in Corpora Allata of the Silkworm, Bombyx mori." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/06617042012513169579.

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30

Liao, Guang-Zheng, and 廖光正. "Studies on the improvement in artificial diet for the young silkworm, bombyx mori." Thesis, 1986. http://ndltd.ncl.edu.tw/handle/92071266568461052125.

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31

TYLLEROVÁ, Helena. "POLYMORFISMUS GENU PRO SERICIN 2 U BOURCE MORUŠOVÉHO (BOMBYX MORI)." Master's thesis, 2010. http://www.nusl.cz/ntk/nusl-53358.

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In our study, we examined the polymorphism of gene Ser2 from domesticated silkmoth Bombyx mori and its closest wild relative B. mandarina. As a starting material for our work, we used the restriction map of allele C isolated from hybrid lineages 200 and 300 of European silkmoth B. mori (Michaille et al. 1990a). We also used the published sequence of allele D which was isolated from {\clq}qDaizo`` p50 strain of B. mori (Kludkiewicz et al 2009). Based on the published sequence, we designed PCR primers and performed sequence analysis of 4 different alleles of gene Ser2, including the almost complete genomic sequence of allele C (except for two short regions containing repetitive DNA). We then compared the sequences and found that the alleles differ significantly not only in intron lengths and the sizes of exon 9, but also in exon arrangements. While the allele C contains 12 exons (1, 2, 3, 4, 5, 6, 7, 8a, 9, 10, 11, 8b), the alelle D and other alleles analysed have 13 exons (1, 2, 3, 4, 5, 6, 7, 8, 9a, 10a, 9b, 10b, 11). Allele C showed very unique arrangement of exons, which suggested that it may represent an ancestral form of Ser2 gene. We analysed the arrangment of exons located at the 3´end of the Ser2 gene by PCR and electrophoresis in 70 available strains of B. mori and B. mandarina. We also tried to find a similar allele to the allele C and we amplified and sequenced 2 kb region from 55 Bombyx strains. Phyllogenetic analysis of gene Ser2 gene suggested that the C allele of now probably extinct European silkmoth hybrids 200 and 300 does not seem to be an ancestral form of Ser2 gene, but it rather seems to be the result of complex secondary rearrangements.

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32

Umashankara, M. L. "Genetic approach for the synthesis of new bivoltine breeds of Silkworm Bombyx Mori,L." Thesis, 2004. http://hdl.handle.net/2009/3077.

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33

Raju, Jagannatha P. "Studies on the hybridization and synthesis of new breeds of silkworm bombyx mori L." Thesis, 1990. http://hdl.handle.net/2009/2693.

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34

Gowda, Puttaswamy. "Studies on some aspects of egg production in silkworm Bombyx mori L. in tropics." Thesis, 1988. http://hdl.handle.net/2009/2694.

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35

SUN, XIANG-YUN, and 孫祥芸. "Infection of celllines of the silkworm, Bombyx mori, with nuclear polyhedrosis virus in vitro." Thesis, 1988. http://ndltd.ncl.edu.tw/handle/76162397323843217225.

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36

Xu, M., X. Wang, J. Tan, K. Zhang, X. Guan, Laurence H. Patterson, H. Ding, and H. Cui. "A novel Lozenge gene in silkworm, Bombyx mori regulates the melanization response of hemolymph." 2015. http://hdl.handle.net/10454/9422.

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No
Runt-related (RUNX) transcription factors are evolutionarily conserved either in vertebrate or invertebrate. Lozenge (Lz), a members of RUNX family as well as homologue of AML-1, functions as an important transcription factor regulating the hemocytes differentiation. In this paper, we identified and characterized RUNX family especially Lz in silkworm, which is a lepidopteran model insect. The gene expression analysis illustrated that BmLz was highly expressed in hemocytes throughout the whole development period, and reached a peak in glutonous stage. Over-expression of BmLz in silkworm accelerated the melanization process of hemolymph, and led to instantaneously up-regulation of prophenoloxidases (PPOs), which were key enzymes in the melanization process. Further down-regulation of BmLz expression by RNA interference resulted in the significant delay of melanization reaction of hemolymph. These findings suggested that BmLz regulated the melanization process of hemolymph by inducing PPOs expression, and played a critical role in innate immunity defense in silkworm.

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37

吳登楨. "Thermotolerance and analyses of heat stable enzymes in the bivoltine silkworm, bombyx mori l." Thesis, 1991. http://ndltd.ncl.edu.tw/handle/83306050657398081837.

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38

YU, XI-JIN, and 余錫金. "Comparative studies on diapause eggs of different strains of the silkworm, Bombyx mori L." Thesis, 1989. http://ndltd.ncl.edu.tw/handle/21089161459893408596.

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39

Prasad, N. R. "Contributions to pathobiology of silkworm Bombyx Mori (L) infected with endoparasite Uzifly. Exorista sorbillans (Wied)." Thesis, 1990. http://hdl.handle.net/2009/2015.

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40

Begum, Naseema A. "Toxicity, sublethal effects and biochemical mode of action of insecticides in the silkworm, Bombyx Mori L." Thesis, 1993. http://hdl.handle.net/2009/1368.

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41

Bhat, D. V. "Food utilization budget and reproductive performance of the Silkworm, Bombyx Mori L. on different Mulberry varieties." Thesis, 2000. http://hdl.handle.net/2009/1849.

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42

Kalappa, H. K. "Studies on the isolation and evolution of High Yielding multivoltine races of silkworm bombyx mori L." Thesis, 1996. http://hdl.handle.net/2009/1926.

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43

Giridhar, K. "Studies on some improved varieties of mulberry and their influence on the silkworm, Bombyx mori L." Thesis, 1996. http://hdl.handle.net/2009/1335.

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44

HONG, RUI-HONG, and 洪瑞宏. "Physical map of bombyx mori nuclear polyhedrosis virus genome and expression of HBsAg gene in silkworm." Thesis, 1990. http://ndltd.ncl.edu.tw/handle/18373659381634653499.

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45

WA, SHI-WEN, and 馬士雯. "Molecular cloning and restriction mapping of polyhedrin gene from nuclear polyhedrosis virus of the silkworm, bombyx mori." Thesis, 1986. http://ndltd.ncl.edu.tw/handle/01725536127293050412.

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46

Chang, Goang-Miao, and 張廣淼. "Infection of silkworm larvae with the recombinant Bombyx mori nuclear polyhedrosis virus containing hepatitis B surface antigen gene." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/35358834899131450441.

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碩士
國立中興大學
昆蟲學系
82
After infecting with the recombinant Bombyx mori nuclear polyhedrosis virus ( BmNPV ), BmHBs324P, which contains hepatitis B surface antigen (HBsAg) genes drived by polyhedrin promoter , the larvae did not show apparent symptom in the initial stage. Later, the infected larvae ceased feeding at 72 hr post-infection. their intersegments became swollen and climbed around the rearing seat. They died at 96 hr post-infection. The dead larvae vomited digestive fluid. The hemolymph of the infected ones became light brown in color. Production of hepatitis B surface antigen in hemolymph was measured after injection of BmHBs324P into 5th instar larvae. The production curve was going up beginning from 60 hr and reached the maximum at 108 hr post-inoculation. The extracellular HBsAg level in BmN cells was similar to that produced in larvae. But intracellular HBsAg increased from 48 hr, and reached the peak at 96 hr post-inoculation. There were several folds differences in HBsAg level between intracellular and extracellular measurements, revealing that the HBsAg proteins were not released out of the cells completely. The HBsAg production in hemolymph increased with an increase in temperature incubating at 15℃ to 30 ℃, but it was very low at 35℃. Injection of 25,000 plaque forming unit( PFU) BmHBs324P into the 5th instar larvae could produce maximun HBsAg in hemolymph. The 5th instar larvae produced the lowest HBsAg at 1 day, but did not show obvious difference from 2 to 5 days after injecting 50,000 PFU into the 5th instar larvae. The HBsAg production in hemolymph of J207 strain was two folds as that of " hwa 2", while variable productions were found among different silkworm strains. These results could be useful for screening high productive strains for producing HBsAg proteins in silkworm larvae.

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47

ZHANG, JUN-KAI, and 張竣凱. "Studies on cellular defense reaction of the silkworm, bombyx mori l., to the white muscardine fungus, beauveria bassiana." Thesis, 1991. http://ndltd.ncl.edu.tw/handle/07642163171339337905.

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48

"Expression and purification of recombinant grass carp (ctenopharyngodon idellus) growth hormone in BmN cells and silkworm (bombyx mori) larvae." Chinese University of Hong Kong, 1994. http://library.cuhk.edu.hk/record=b5895461.

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Poon, Chi-to, Geoffrey.
Thesis (M.Phil.)--Chinese University of Hong Kong, 1994.
Includes bibliographical references (leaves 115-125).
Acknowledgements --- p.I
Abbreviations --- p.II
Abstract --- p.III
Table of content --- p.IV
Chapter Chapter 1 --- Introduction --- p.1
Chapter 1.1 --- Importance of growth enhancement in aquaculture --- p.1
Chapter 1.2 --- Physiological effect of growth hormone --- p.1
Chapter 1.3 --- Progress in teleost growth hormone research --- p.3
Chapter 1.4 --- Grass carp and its aquaculture --- p.5
Chapter 1.5 --- Route of administration of growth hormone --- p.8
Chapter 1.6 --- Nomenclature of baculovirus --- p.9
Chapter 1.7 --- Biology of baculovirus --- p.10
Chapter 1.8 --- Control of gene expression of virus-infected cells --- p.13
Chapter 1.9 --- Theme of the thesis --- p.14
Chapter Chapter 2 --- Materials and Methods --- p.18
Chapter 2.1 --- Synthesis and purification of primers --- p.18
Chapter 2.2 --- Modification of gcGH cDNA by polymerase chain reaction (PCR) --- p.20
Chapter 2.3 --- TA cloning of PCR product --- p.20
Chapter 2.4 --- Purification ofDNA fragment from agarose gel by GENECLEAN´ёØ --- p.21
Chapter 2.5 --- Recovery of low molecular weight DNA fragment from agarose gel --- p.22
Chapter 2.6 --- Small scale preparation of plasmid DNA --- p.23
Chapter 2.7 --- Large scale plasmid preparation by QIAGEN´ёØ --- p.24
Chapter 2.8 --- Preparation of competent Escherichia coli JM109 for transformation --- p.25
Chapter 2.9 --- Transformation of plasmid into competent Escherichi coli JM109 --- p.26
Chapter 2.10 --- Cell culture of BmN cell line --- p.26
Chapter 2.10.1 --- Preparation of TC-100 insect medium --- p.27
Chapter 2.10.2 --- Preparation of Grace's medium --- p.27
Chapter 2.11 --- Extraction of wild-type Bombyx mori nuclear polyhedrosis virus DNA --- p.28
Chapter 2.12 --- Transfection of BmN cells with Bombyx mori nuclear polyhedrosis virus DNA by DOTAP´ёØ --- p.28
Chapter 2.13 --- Agarose plaque assay --- p.29
Chapter 2.14 --- Lifting of vius plaque onto nitrocellulose filter paper --- p.30
Chapter 2.15 --- Synthesis of radiolabelled DNA probe --- p.31
Chapter 2.16 --- Pre-hybridization and hybridization of recombinant virus DNA on nitrocellulose paper --- p.31
Chapter 2.17 --- Purification of recombinant virus by dot-blot manifold --- p.33
Chapter 2.18 --- Preparation of cell lysate from virus-infected BmN cells --- p.33
Chapter 2.19 --- Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) --- p.34
Chapter 2.19.1 --- Staining of the gel by Coomassie blue method --- p.35
Chapter 2.19.2 --- Staining of the gel by silver staining method --- p.35
Chapter 2.20 --- Determination of protein concentration by Bradford's method --- p.36
Chapter 2.21 --- Determination of total protein concentration by Folin-Lowry method --- p.36
Chapter 2.22 --- Detection of grass carp growth hormone by Western blotting --- p.37
Chapter 2.23 --- Preparation of native recombinant grass carp growth hormone for iodination --- p.38
Chapter 2.24 --- Iodination of recombinat grass carp growth hormone by IODO-GEN´ёØ --- p.38
Chapter 2.25 --- Purification of radiolabelled recombinant grass carp growth hormone --- p.39
Chapter 2.26 --- Radioimmunoassay (RIA) for detection of recombinant grass carp growth hormone --- p.40
Chapter 2.27 --- Ammonium sulphate precipitation --- p.41
Chapter Chapter 3 --- Vector Construction --- p.42
Chapter 3.1 --- Components of parent vector pBM030 --- p.42
Chapter 3.2 --- Construction of pBM-EE --- p.44
Chapter 3.3 --- Constrcution of pBM-EX --- p.47
Chapter Chapter 4 --- Results --- p.51
Chapter 4.1 --- Construction and purfication of recombinant baculovirus --- p.51
Chapter 4.2 --- Expression of recombinant grass carp growth hormone in BmN cells --- p.55
Chapter 4.3 --- Expression of recombinant grass carp growth hormone in Bombyx mori larva --- p.62
Chapter 4.4 --- Putative physical characteristics of the recombinant grass carp growth hormone --- p.67
Chapter 4.5 --- Purification of the grass carp growth hormone in Bombyx mori larva --- p.69
Chapter 4.5.1 --- Ammonium sulphate precipitation --- p.69
Chapter 4.5.2 --- Gel filtration --- p.72
Chapter 4.5.3 --- Hydrophobic interaction chromatography --- p.75
Chapter 4.5.4 --- Anion exchange chromatography --- p.78
Chapter 4.5.5 --- Reverse phase chromatography --- p.90
Chapter Chapter 5 --- Discussions --- p.99
Chapter 5.1 --- Merits of baculovirus expression system against other expression systems --- p.99
Chapter 5.2 --- Basic design of the recombinant baculovirus transfer vector --- p.100
Chapter 5.3 --- Potential for Mutation of the Baculovirus during Homologous Recombination --- p.101
Chapter 5.4 --- Cleavage of Signal Peptide from the Expressed Protein --- p.103
Chapter 5.5 --- Difference in recombinant gcGH expression levelin EE4-7 and EX3-16 --- p.103
Chapter 5.6 --- Purification of recombinant gcGH protein --- p.106
Chapter 5.6.1 --- Chromatographic behaviour of recombinant gcGH in Q-Sepharose column --- p.106
Chapter 5.6.2 --- Problem of aggregation of recombinant gcGH --- p.107
Chapter 5.6.3 --- Solvent system used in recombinant gcGH purification --- p.108
Chapter 5.6.4 --- Protein denaturating effect of the solvent system --- p.109
Chapter 5.6.5 --- Protein yield --- p.110
Chapter 5.7 --- Problems and accuracy of radioimmunoassay --- p.110
Chapter Chapter 6 --- Further study --- p.113
Chapter Chapter 7 --- References --- p.115
Appendix I --- p.126
Appendix II: Construction of the Supervector --- p.127

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49

Chandrashekharaiah. "Studies on the genetics of quantitative traits in a few multivoltine and bivoltine races of silkworm Bombyx. Mori L." Thesis, 1992. http://hdl.handle.net/2009/1408.

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50

Singh, Anju. "Identification and characterization of a highly conserved gene, which is predominantly expressed in the human testis, in silkworm Bombyx mori." Thesis, 2000. http://hdl.handle.net/2009/803.

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