Relevant bibliographies by topics / Bombyx mori silkworm

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Academic literature on the topic 'Bombyx mori silkworm'

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Published: 4 June 2021
Last updated: 10 February 2022

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Journal articles on the topic "Bombyx mori silkworm"

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G. SAVITHRI, G. SAVITHRI, P. SUJATHAMMA P. SUJATHAMMA, and V. Asha Krishna. "Silkworm Bombyx Mori – An Economic Insect." International Journal of Scientific Research 2, no. 7 (June 1, 2012): 535–37. http://dx.doi.org/10.15373/22778179/july2013/187.

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Micheal, Ann Sandhya, and Muthangi Subramanyam. "Stressors Induced Antioxidant System in Silkworm Bombyx Mori." International Journal of Scientific Research 3, no. 7 (June 1, 2012): 1–2. http://dx.doi.org/10.15373/22778179/july2014/174.

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Song, Wenting, Yixuan Fan, Feifei Zhu, Rehab Hosny Taha, and Keping Chen. "The Expression of UGT46A1 Gene and Its Effect on Silkworm Feeding." Processes 9, no. 8 (August 23, 2021): 1473. http://dx.doi.org/10.3390/pr9081473.

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The silkworm, Bombyx mori, uses a complex olfactory system to determine whether the food is edible. As an odor degrading enzyme, UDP-glycosyltransferase (UGT) participates in the degradation of odor molecules in the olfactory system of the silkworm. By sequencing the whole genome of the silkworm NB and using comparative genomics methods, we found that UGT46A1 is unique in species that eat mulberry leaves. Bioinformatics shows that its function may be related to the feeding habits of the silkworm. In this study, it was found through quantitative real-time polymerase chain reaction (qRT-PCR) that UGT46A1 was highly expressed in the heads of silkworms, which was consistent with the conjecture that UGT46A1 was involved in silkworm olfactory recognition. RNA interference (RNAi) was used to knock down the expression of UGT46A1. By observing the silkworm’s tendency toward mulberry leaves and food selectivity, it was found that the silkworms that successfully knocked down the UGT46A1 gene altered their feeding habits and that their ability to find food was weakened, but they could eat more leaves of plants other than mulberry leaves. This evidence indicates that UGT46A1 may affect the silkworm’s feeding by influencing the olfactory system of the silkworm.
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Qian, Heying, Gang Li, Guodong Zhao, Mingzhu Liu, and Anying Xu. "Metabolic Characterisation of the Midgut of Bombyx mori Varieties after BmNPV Infection Using GC-MS-Based Metabolite Profiling." International Journal of Molecular Sciences 21, no. 13 (July 1, 2020): 4707. http://dx.doi.org/10.3390/ijms21134707.

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Bombyx mori nucleopolyhedrovirus (BmNPV) is a silkworm disease that is especially harmful to cocoon production and seriously restricts sericultural development. Our laboratory successfully cultivated a new highly BmNPV-resistant silkworm variety, Huakang 2; however, its mechanism of BmNPV resistance remains unclear. To understand its resistance mechanism, we conducted a metabolomic and transcriptomic study of the midgut of silkworm varieties, Baiyu N and Baiyu after BmNPV infection. We identified 451 differential metabolites, which were mostly comprised of small molecules, such as saccharides, acids, amines, alcohols, and glycosides. We found that the primary differences in disease resistance between the silkworm varieties are metabolic-pathways, tryptophan metabolism, oxidative phosphorylation, ABC-transporters, beta-alanine metabolism, and phenylalanine metabolism. Combined analysis with transcriptomic data suggested that tryptophan metabolism and oxidative phosphorylation are closely related to the silkworms’ BmNPV resistance. We hypothesize that the roles of the two metabolic pathways in the BmNPV resistance mechanism might be the following: Oxidative phosphorylation generates a large amount of adenosine triphosphate (ATP) in response to BmNPV infection to provide silkworms the energy required for establishing BmNPV resistance. Tryptophan metabolism then activates the aryl hydrocarbon receptor (AhR) through the exogenous virus BmNPV, which activates the silkworm’s immune system to defeat BmNPV infections.
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Zhao, Erhu, Xiaolan Jiang, and Hongjuan Cui. "Bombyx mori Dihydroorotate Dehydrogenase: Knockdown Inhibits Cell Growth and Proliferation via Inducing Cell Cycle Arrest." International Journal of Molecular Sciences 19, no. 9 (August 30, 2018): 2581. http://dx.doi.org/10.3390/ijms19092581.

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Dihydroorotate dehydrogenase (DHODH), in the de novo pyrimidine biosynthetic pathway, is the fourth enzyme of pyrimidine synthesis and is used to oxidize dihydroorotate and hence to orotat. We cloned and characterized here the dhod of silkworms, Bombyx mori. The full-length cDNA sequence of dhod is 1339 bp, including an open reading frame (ORF) of 1173 bp that encoded a 390 amino acid protein. And two domains were involved in the Dihydroorotate dehydrogenase amino acid sequence of silkworms, Bombyx mori (BmDHODH), namely a DHO_dh domain and a transmembrane domain in N-termina. The silkworm dhod is expressed throughout development and in nine tissues. Moreover, knockdown of the silkworm dhod gene reduced cell growth and proliferation through G2/M phase cell cycle arrest. Similarly, DHODH inhibitor (leflunomide) also reduced cell growth and proliferation, with a significant decrease of cyclin B and cdk2. DHODH is the fourth enzyme of pyrimidine synthesis, so we also found that leflunomide can inhibit, at least in part, the endomitotic DNA replication in silk glands cells. These findings demonstrate that downregulation of BmDHODH inhibits cell growth and proliferation in silkworm cells, and the endomitotic DNA replication in silk gland cells.
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Xu, Jun, Qinglin Dong, Ye Yu, Baolong Niu, Dongfeng Ji, Muwang Li, Yongping Huang, Xin Chen, and Anjiang Tan. "Mass spider silk production through targeted gene replacement in Bombyx mori." Proceedings of the National Academy of Sciences 115, no. 35 (August 6, 2018): 8757–62. http://dx.doi.org/10.1073/pnas.1806805115.

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Spider silk is one of the best natural fibers and has superior mechanical properties. However, the large-scale harvesting of spider silk by rearing spiders is not feasible, due to their territorial and cannibalistic behaviors. The silkworm, Bombyx mori, has been the most well known silk producer for thousands of years and has been considered an ideal bioreactor for producing exogenous proteins, including spider silk. Previous attempts using transposon-mediated transgenic silkworms to produce spider silk could not achieve efficient yields, due to variable promoter activities and endogenous silk fibroin protein expression. Here, we report a massive spider silk production system in B. mori by using transcription activator-like effector nuclease-mediated homology-directed repair to replace the silkworm fibroin heavy chain gene (FibH) with the major ampullate spidroin-1 gene (MaSp1) in the spider Nephila clavipes. We successfully replaced the ∼16-kb endogenous FibH gene with a 1.6-kb MaSp1 gene fused with a 1.1-kb partial FibH sequence and achieved up to 35.2% chimeric MaSp1 protein amounts in transformed cocoon shells. The presence of the MaSp1 peptide significantly changed the mechanical characteristics of the silk fiber, especially the extensibility. Our study provides a native promoter-driven, highly efficient system for expressing the heterologous spider silk gene instead of the transposon-based, random insertion of the spider gene into the silkworm genome. Targeted MaSp1 integration into silkworm silk glands provides a paradigm for the large-scale production of spider silk protein with genetically modified silkworms, and this approach will shed light on developing new biomaterials.
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Yu, Lin-Lin, Ying-Jun Cui, Guo-Jun Lang, Ming-Yan Zhang, and Chuan-Xi Zhang. "The ionotropic γ-aminobutyric acid receptor gene family of the silkworm, Bombyx mori." Genome 53, no. 9 (September 2010): 688–97. http://dx.doi.org/10.1139/g10-056.

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γ-Aminobutyric acid (GABA) is a very important inhibitory neurotransmitter in both vertebrate and invertebrate nervous systems. GABA receptors (GABARs) are known to be the molecular targets of a class of insecticides. Members of the GABAR gene family of the silkworm, Bombyx mori , a model insect of Lepidoptera, have been identified and characterized in this study. All putative silkworm GABAR cDNAs were cloned using the reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Bombyx mori appears to have the largest insect GABAR gene family known to date, including three RDL, one LCCH3, and one GRD subunit. The silkworm RDL1 gene has RNA-editing sites, and the RDL1 and RDL3 genes possess alternative splicing. These mRNA modifications enhance the diversity of the silkworm’s GABAR gene family. In addition, truncated transcripts were found for the RDL1 and LCCH3 genes. In particular, the three RDL subunits may have arisen from two duplication events.
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Tang, Linmeng, Dehong Yang, Yaohui Wang, Xu Yang, Kai Chen, Xingyu Luo, Jun Xu, et al. "5′-Nucleotidase Plays a Key Role in Uric Acid Metabolism of Bombyx mori." Cells 10, no. 9 (August 30, 2021): 2243. http://dx.doi.org/10.3390/cells10092243.

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Uric acid (UA) is the end-product in the human purine metabolism pathway. The UA that accumulates in silkworm tissues is excreted as a nitrogen waste product. Here, we first validated that Bombyx mori has a homolog of the human gene that encodes the 5′-nucleotidase (5′N) involved in purine metabolism. The B. mori gene, Bm5′N, is located upstream of other genes involved in UA metabolism in the silkworm. Disruption of Bm5′N via the CRISPR/Cas9 system resulted in decreased UA levels in the silkworm epidermis and caused a translucent skin phenotype. When Bm5′N mutant silkworms were fed with the uric acid precursor inosine, the UA levels in the epidermis increased significantly. Furthermore, the metabolomic and transcriptomic analyses of Bm5′N mutants indicated that loss of the Bm5′N affected purine metabolism and the ABC transport pathway. Taken together, these results suggest that the UA pathway is conserved between the silkworm and humans and that the Bm5′N gene plays a crucial role in the uric acid metabolism of the silkworm. Thus, the silkworm may be a suitable model for the study of UA metabolism pathways relevant to human disease.
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Rudramuni, Kiran, Shiv Kumar, Bharath K. Neelaboina, Mir N. Ahmad, and Sukhen R. Chowdhury. "Atypical voltinism in mulberry silkworm Bombyx mori L., 1758 (Lepidoptera: Bombycidae) races Barapat and Barapolu." Entomological Communications 3 (January 4, 2021): ec03001. http://dx.doi.org/10.37486/2675-1305.ec03001.

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The mulberry silkworm, Bombyx mori L., 1758 (Lepidoptera: Bombycidae) is classified as univoltine, bivoltine, and polyvoltine based on the annual brood frequency. Voltinism in B. mori is characterized by the occurrence of embryonic diapause (facultative and obligatory diapause). Univoltine silkworms suited to cold and temperate regions lay diapausing eggs and polyvoltine silkworms suited to tropical regions lay non-diapausing eggs. In contrast, the occurrence of diapause in bivoltine silkworms is influenced by the environment. In addition to the difference in the occurrence of embryonic diapause, larval duration, and cocoon characters show distinct variations. In the present correspondence, we are presenting an interesting observation from two silkworm races Barapath and Barapolu originating from the North-East region of India that display divergence in voltinism. The unique characteristic features of univoltine, bivoltine, and polyvoltine observed in the races are discussed explaining the occurrence.
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Ito, Katsuhiko, Kangayam M. Ponnuvel, and Keiko Kadono-Okuda. "Host Response against Virus Infection in an Insect: Bidensovirus Infection Effect on Silkworm (Bombyx mori)." Antioxidants 10, no. 4 (March 27, 2021): 522. http://dx.doi.org/10.3390/antiox10040522.

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Silk cocoons obtained from silkworms are the primary source of commercial silk, making the silkworm an economically important insect. However, the silk industry suffers significant losses due to various virus infections. Bombyx mori bidensovirus (BmBDV) is one of the pathogens that cause flacherie disease in silkworms. Most silkworm strains die after BmBDV infection. However, certain silkworm strains show resistance to the virus, which is determined by a single recessive gene, nsd-2. The +nsd-2 gene (allele of nsd-2; the susceptibility gene) encodes a putative amino acid transporter expressed only in the insect’s midgut, where BmBDV can infect, suggesting that this membrane protein may function as a receptor for BmBDV. Interestingly, the expression analysis revealed no changes in the +nsd-2 gene expression levels in virus-uninfected silkworms, whereas the gene expression drastically decreased in the virus-infected silkworm. This condition indicates that the host factor’s expression, the putative virus receptor, is affected by BmBDV infection. It has recently been reported that the expression levels of some host genes encoding cuticle, antioxidant, and immune response-related proteins were significantly regulated by BmBDV infection. In this review, we discuss the host response against virus infection based on our knowledge and long-term research experience in this field.
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Dissertations / Theses on the topic "Bombyx mori silkworm"

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Åberg, Gabriel. "Intrinsically Functionalized Silk (Bombyx Mori)." Thesis, Linköpings universitet, Institutionen för fysik, kemi och biologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-97636.

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The goal of the thesis is to incorporate materials with either fluorescent or conductive properties in silk fibers, by feeding silkworms with a diet containing these materials. To achieve this, one would have to breed (rear) silkworm from eggs into larvaes, then to feed the silkworms with this special diet containing fluorescent or conductive materials. Samples of silk were then collected either from spun cocoons or via removing the silk producing organs (silk glands) from the silkworms via dissection. The samples were then analyzed with absorbance spectrometer, spectrofluorometer or via photoluminesecent measurement to determine if any materials had been incorporated into the silk fibers.   Silkworms were successfully reared from eggs up to moths, once the silkworm larvae had grown enough in size their diet were switches from their regular food (silkworm chow) to food containing conjugated molecules or polymers with fluorescent or conductive properties. A total of 14 materials were tested. One material gave a clear positive result and that was from the fluorescent compound Rhodamine B. Other fluorescent materials, Nile red and POWT yielded some results indicating their presence in the silk but the results were not conclusive. The rest of the materials all failed with being incorporated within the silk fibers; this was due to their lethality, size, lack of zwitterionic properties and such.  The properties of the materials are of great importance for the uptake process, where a small zwitterionic molecule has a great change of being taken up and incorporated in the silk fibers. Whereas a big materials such as a polymer without any zwitterionic will in most cases just follow through the food in the digestive track without any uptake.
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Trancik, Jessika. "Silk microstructures." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249173.

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Blossman-Myer, Bonnie. "A Contravention of Established Principles of Interspecific Allometric Metabolic Scaling in Developing Silkworms, Bombyx Mori." Thesis, University of North Texas, 2007. https://digital.library.unt.edu/ark:/67531/metadc3704/.

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Established interspecific metabolic allometric relationships do not adequately describe the complexity and variable physiological states of developing animals. Consequently, intraspecific allometric relationships of oxygen consumption and carbon dioxide production as a function of body mass; the respiratory quotient; the function of the silk cocoon; and body composition were investigated for each distinct developmental stage of the silkworm, Bombyx mori. Whole animal O2 consumption in Bombyx ranged from 0.00064 + 0.000047 ml O2 .hr-1 at larval instar I to 0.77 + 0.06 ml O2 .hr-1 in pre-pupal, falling to 0.21+ 0.01 ml O2 .hr-1 in the pupae. Those instars having a significant relationship between O2 consumption as a function of body mass, the slope of the line relating O2 consumption to body mass varied between 0.99 and 1.02, while across all instars the slope was 0.82. Developmental allometry should be presented for individual developmental stages because the individual allometric exponents of the stages can be significantly different from the overall allometric exponent throughout development and in some cases, the overall allometric exponent can be a statistical artifact. The first larval instar of Bombyx mori has the lowest cross sectional area of high metabolic tissue of the midgut (27%) and had one of the highest percentages of some metabolically inert tissues (i.e. lipid, 7.5%). Body composition of the first instar does not support the idea that smaller mass animals having the highest O2 consumption are composed of a greater percentage of metabolically active organs when compared to larger animals. However, this developmental stage has the highest percentage of the mitochondrial marker cytochrome oxidase, which correlates well with the high O2 consumption rate of the smaller mass. Therefore, established interspecific principles should not be assumed to function as valid models for intraspecific developmental relationships of metabolism as a function of body mass. Developmental allometry should include an analysis of individual stages of development as well as an analysis of development as a whole to gain a comprehensive understanding of the complexity of allometry of the developing animal such as the silkworm.
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Begum, Hosne Ara. "Hybridization of mulberry silkworm (Bombyx Mori L.) for higher silk productivity and disease resistance /." [St. Lucia, Qld.], 2006. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe19789.pdf.

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Santorum, Marilucia. "Avaliação da toxicidade do inseticida novaluron em Bombyx mori (Lepidoptera Bombycidae) /." Botucatu, 2019. http://hdl.handle.net/11449/182490.

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Orientador: Daniela Carvalho dos Santos
Resumo: O bicho-da-seda, Bombyx mori (Lepidoptera: Bombycidae), é o inseto de maior importância econômica na produção de seda. A lagarta se alimenta de folhas de amoreira e é altamente sensível a agrotóxicos, assim o uso destes em culturas agrícolas circunvizinhas às plantações de amoreira pode afetar o desenvolvimento de B. mori, acarretando em desequilíbrio nas suas funções metabólicas e, consequentemente, comprometendo a produção de casulos. Entre estes agrotóxicos, destaca-se o Novaluron, inseticida inibidor da síntese de quitina nos insetos e empregado no controle de insetos pragas de culturas agrícolas próximas as plantações de amoreira. Assim, investigamos os efeitos tóxicos de Novaluron no desenvolvimento de B. mori. Lagartas de B. mori, foram separadas em dois grupos experimentais: grupo controle (GC) e grupo tratamento (GT: tratado com 0, 15 mL/L de Novaluron). Após ecdise do 2° para o 3° instar, lagartas do GT foram alimentadas por 24 horas com folhas de amoreira tratadas com o inseticida. Paralelamente foi realizada uma nova exposição, porém em lagartas que realizavam a ecdise do 4° para o 5° instar. Lagartas, pupas e adultos de B. mori foram anestesiadas e segmentos do intestino médio, glândula da seda e órgãos reprodutores retirados e processados convencionalmente para técnicas de microscopias de luz, eletrônica e imunohistoquímica. Além disso, os efeitos no desenvolvimento, reprodução e qualidade do casulo também foram avaliados. O Novaluron provocou efeitos citotóxico... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The silkworm, Bombyx mori (Lepidoptera: Bombycidae is the insect of major economic importance in the production of silk. The larvae feeds on mulberry leaves and is highly sensitive to agrochemicals, thus the use of these in agricultural crops surrounding the mulberry plantations can affect the development of B. mori, causing an imbalance in its metabolic functions and, consequently, compromising the production of cocoons. Among these agrochemicals, stands out the Novaluron, an insecticide inhibitor of the synthesis of chitin in insects and used in the control of insect pests of crops near mulberry plantations. Thus, we investigated the lethal and sublethal effects of Novaluron on the development of B. mori. Larvae were selected into two experimental groups: control group (CG) and treatment group (TG: treated with 0, 15 mL/L Novaluron). After ecdysis from the 2nd to the 3rd instar, the TG larvae were fed for 24 hours with mulberry leaves treated with the insecticide. In parallel, a new exposition was carried out, however in larvae that carry out the ecdysis from the 4th to the 5th instar. B. mori larvae, pupae and adults were anesthetized and segments of the midgut, silk gland and reproductive organs were removed and processed conventionally for light microscopy, electron microscopy and immunohistochemistry. In addition, the effects on the development, reproduction and quality of the cocoon were also evaluated. Novaluron caused cytotoxic effects on the midgut and the silk glan... (Complete abstract click electronic access below)
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Krasnov, Igor [Verfasser]. "Mechanical properties of Bombyx mori silkworm silk: viscoelasticity, structural and molecular origin / Igor Krasnov." Kiel : Universitätsbibliothek Kiel, 2013. http://d-nb.info/1045604046/34.

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Lunke, Martine Dominique. "Molecular characterization of BmGATA-beta, a gene involved in choriogenesis in the silkworm Bombyx mori." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0027/NQ49515.pdf.

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Rao, Sudhakara P. "Studies on the evolution of adaptive bivoltine breeds of silkworm bombyx mori L. for tropical climates." Thesis, Studies on the evolution of adaptive bivoltine breeds of silkworm bombyx mori L. for tropical climates, 2003. http://hdl.handle.net/2009/3159.

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Diao, Yupu, Anrui Lu, Bing Yang, Wenli Hu, Qing Peng, Qing-Zhi Ling, Brenda T. Beerntsen, Kenneth Söderhäll, and Erjun Ling. "Existence of Prophenoloxidase in Wing Discs : A Source of Plasma Prophenoloxidase in the Silkworm, Bombyx mori." Uppsala universitet, Jämförande fysiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-184482.

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In insects, hemocytes are considered as the only source of plasma prophenoloxidase (PPO). PPO also exists in the hemocytes of the hematopoietic organ that is connected to the wing disc of Bombyx mori. It is unknown whether there are other cells or tissues that can produce PPO and release it into the hemolymph besides circulating hemocytes. In this study, we use the silkworm as a model to explore this possibility. Through tissue staining and biochemical assays, we found that wing discs contain PPO that can be released into the culture medium in vitro. An in situ assay showed that some cells in the cavity of wing discs have PPO1 and PPO2 mRNA. We conclude that the hematopoietic organ may wrongly release hemocytes into wing discs since they are connected through many tubes as repost in previous paper. In wing discs, the infiltrating hemocytes produce and release PPO probably through cell lysis and the PPO is later transported into hemolymph. Therefore, this might be another source of plasma PPO in the silkworm: some infiltrated hemocytes sourced from the hematopoietic organ release PPO via wing discs.
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Santorum, Marilucia. "Infecção de células da ampola dos túbulos de MALPIGHI de Bombyx Mori pelo Alphabaculovirus." Universidade Estadual do Oeste do Parana, 2015. http://tede.unioeste.br:8080/tede/handle/tede/650.

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Made available in DSpace on 2017-07-10T14:17:10Z (GMT). No. of bitstreams: 1 AMPOLA DOS _ MALPIGHI DE Bombyx mori PELO ALPHABACULOVIRUS.pdf: 2176663 bytes, checksum: 866566cf54573cb621db0597bbb30516 (MD5) Previous issue date: 2015-02-24
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Bombyx mori nucleopolyhedrovirus (BmNPV) is an entomopathogenic virus Baculoviridae family, Alphabaculovirus genre, which infects the silkworm, Bombyx mori and causes nuclear polyhedrosis disease. A viral geographic isolated from BmNPV was identified in the state of Paraná, Brazil, and a number of organs and target tissues were identified, but there is no information about the infection of Malpighian tubules (MT). MT make up the excretory system of B. mori, acting in the elimination of toxic substances and electrolyte homeostasis. This study aimed to analyze the behavior on the susceptibility and cytopathology of MT B. mori to BmNPV. Therefore, hybrid B.mori caterpillars at 5º instar, were inoculated experimentally with a viral suspension of BmNPV. On different days post-inoculation (dpi), the 4° to 9° dpi, MT segments were collected, divided in regions of the ampullae, proximal, medial and distal; following the processing for light and electron microscopy transmission. Material control followed the same process that the inoculated. The results showed that B. mori MT showed variations in their morphology and susceptibility to BmNPV according to the analyzed region. Thus, the region of the ampullae in its transition area to the proximal, appeared susceptible to BmNPV, from 6° dpi, while other regions showed no evidence of infection by the pathogen. The transition area is a small region which is located at the end of the ampullae and the beginning of the proximal region of MT. The cellular morphology of simple lining epithelium showed continuous variation of pavement, characteristic of the ampullae, the cylindrical, in the transition area; already continuous with the proximal region of MT, this morphological change was abruptly. The cytopathology revealed in hypertrophic nucleus, viroplasm containing the nucleocapsid and viral polyhedra. At the end of the infectious cycle was observed cytolysis of infected cells, releasing polyhedra in the extracellular medium, both in hemocele as in MT lumen, a factor that may favor the transmission of the disease. Thus, infection of the ampullae in its transition area to the proximal region, will contribute to greater scientific knowledge of cytopathology aspects and targets of infection from this virus isolated geographical, for the establishment of its infectious cycle of the insect's body BmNPV
Bombyx mori nucleopolyhedrovirus (BmNPV) é um vírus entomopatogênico da família Baculoviridae, gênero Alphabaculovirus, que infecta o bicho-da-seda, Bombyx mori (Lepidoptera: Bombycidae) e causa a doença poliedrose nuclear. Um isolado geográfico viral do BmNPV foi identificado no estado do Paraná, Brasil, e uma série de órgãos e tecidos alvos foram identificados, porém não há informações sobre a infecção dos túbulos de Malpighi (TM). Os TM compõem o sistema excretor de B. mori, atuando na eliminação de substâncias tóxicas e na homeostase hidroeletrolítica. Assim, este estudo objetivou analisar o comportamento referente à susceptibilidade e citopatologia dos TM de B. mori ao BmNPV. Para tanto, lagartas híbridas de B. mori, 5° instar, foram inoculadas experimentalmente com uma suspensão viral de BmNPV. Em diferentes dias pós-inoculação (dpi), do 4° ao 9° dpi, segmentos dos TM foram coletados, subdivididos nas regiões da ampola, proximal, média e distal; seguindo o processamento para as microscopias de luz e eletrônica de transmissão. Material controle seguiu o mesmo processamento que o inoculado. Os resultados demonstraram que os TM de B. mori, apresentaram variação na sua morfologia e susceptibilidade ao BmNPV de acordo com a região analisada. Assim, a região da ampola, na sua área de transição com a proximal, se apresentou susceptível ao BmNPV, a partir do 6° dpi, enquanto as demais regiões não evidenciaram indícios de infecção pelo patógeno. A área de transição é uma pequena região que se localiza ao término da ampola e inicio da região proximal do TM. A morfologia celular do epitélio de revestimento simples apresentou variação continua de pavimentoso, característico da ampola, a cilíndrico, na sua área de transição; já na continuidade com a região proximal do TM, esta variação morfológica foi abrupta. A citopatologia revelou no núcleo hipertrófico, o viroplasma, contendo os nucleocapsídeos e os poliedros virais. Ao final do ciclo infeccioso foi verificado a citólise de células infectadas, liberando poliedros no meio extracelular, tanto na hemocele como no lúmen do TM, fator que pode favorecer a transmissão da doença. Assim, a infecção da ampola, na sua área de transição com a região proximal, irá contribuir com um maior conhecimento científico dos aspectos citopatológicos e dos alvos de infecção deste isolado geográfico viral, visando o estabelecimento do seu ciclo infeccioso do BmNPV no corpo do inseto.
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Books on the topic "Bombyx mori silkworm"

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Inc, Game Counsellor, ed. The Game Counsellor's answer book for Nintendo Game players: Hundredsof questions -and answers - about more than 250 popular Nintendo Games. Redmond, Washington: Microsoft Press, 1991.

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Book chapters on the topic "Bombyx mori silkworm"

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Klimenko, V. V. "The Silkworm Bombyx mori." In Animal Species for Developmental Studies, 231–51. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0503-3_9.

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Mundkur, Rajendra, and E. Muniraju. "Molecular Marker-Assisted Selection Breeding in Silkworm, Bombyx mori." In Trends in Insect Molecular Biology and Biotechnology, 3–34. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-61343-7_1.

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Kato, Tatsuya, and Enoch Y. Park. "Advanced Protein Expression Using Bombyx mori Nucleopolyhedrovirus (BmNPV) Bacmid in Silkworm." In Short Views on Insect Genomics and Proteomics, 165–84. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-24244-6_7.

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Chen, Q., L. Li, Z. Yu, and J. Peng. "Establishment of Cell Line from Embryos of the Silkworm, Bombyx Mori." In Invertebrate and Fish Tissue Culture, 259–61. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73626-1_61.

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Zuluaga-Velez, A., R. Buitrago-Sierra, J. F. Santa, F. A. Tabares-Villa, E. Aguilar, and J. C. Sepúlveda-Arias. "Synthesis and characterization of nanofibroin hydrogels from Colombian silkworm Bombyx Mori L." In VII Latin American Congress on Biomedical Engineering CLAIB 2016, Bucaramanga, Santander, Colombia, October 26th -28th, 2016, 732–36. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-4086-3_184.

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Murakami, A., Y. Ohtsuki, and T. Kitazawa. "Whole Embryonic Culture of Spontaneous Parthenogenetic Embryos in the Silkworm, Bombyx Mori." In Invertebrate and Fish Tissue Culture, 64–67. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73626-1_17.

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Imanishi, S., S. Sato, and T. Mori. "Characteristics of Cell Lines Derived from Embryos in The Silkworm, Bombyx Mori." In Invertebrate and Fish Tissue Culture, 255–58. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73626-1_60.

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Ponnuvel, Kangayam M., Sirigineedi Sasibhushan, N. Murthy Geetha, and CGP Rao. "Diapause-Related Gene Expression in Eggs of Multivoltine Bombyx mori L. Silkworm Races." In New Horizons in Insect Science: Towards Sustainable Pest Management, 187–98. New Delhi: Springer India, 2015. http://dx.doi.org/10.1007/978-81-322-2089-3_18.

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Muniraju, E., and Rajendra Mundkur. "Tracing of Evolution in Silkworm, Bombyx mori L., on the Basis of Molecular Studies." In Trends in Insect Molecular Biology and Biotechnology, 67–84. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-61343-7_4.

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Daimon, Takaaki. "Highly Efficient Targeted Gene Disruption in the Silkworm, Bombyx mori, Using Genome Editing Tools." In Targeted Genome Editing Using Site-Specific Nucleases, 81–96. Tokyo: Springer Japan, 2014. http://dx.doi.org/10.1007/978-4-431-55227-7_5.

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Conference papers on the topic "Bombyx mori silkworm"

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Nwibo, Daniel Don. "Infectious disease ofEnterococcus mundtiiin silkworm,Bombyx mori." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.109886.

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Wyman, Aaron J., and Mary Alice Webb. "Calcium Oxalate Accumulation in Malpighian Tubules of Silkworm (Bombyx mori)." In RENAL STONE DISEASE: 1st Annual International Urolithiasis Research Symposium. AIP, 2007. http://dx.doi.org/10.1063/1.2723606.

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Kiuchi, Takashi. "Genome editing in the masculinizing gene of the silkworm Bombyx mori." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.94493.

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Roller, Ladislav, Yoshiaki Tanaka, Ivana Valachová-Spálovská, Ladislav Šimo, and Dušan Žitňan. "The analysis of neuropeptides encoded in the silkworm (Bombyx mori) genome." In Xth Conference Biologically Active Peptides. Prague: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2007. http://dx.doi.org/10.1135/css200709083.

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Tabunoki, Hiroko. "Can the silkworm (Bombyx mori) be used as a human disease model?" In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.112440.

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Lyu, Peng. "Effects of caloric restriction on immune responses againstStaphylocuccus aureusin silkworm,Bombyx mori." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.113454.

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Deng, Xiao-Juan. "Regulation of antimicrobial genes via insulin-like signaling pathway in silkworm,Bombyx mori." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.108150.

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Sezutsu, Hideki. "Development of genome editing tools and their applications in the silkworm,Bombyx mori." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.94026.

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Fujii, Tsuguru. "Practical use of a long term preservation method in the silkworm,Bombyx mori." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.111717.

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Wu, Jinmei. "Integument constitutes an active component of innate immunity in the silkworm,Bombyx mori." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.113582.

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