Dissertations / Theses on the topic 'Blood – Collection and preservation – Australia'

The Australian Red Cross Blood Transfusion Service (ARCBTS) in Western Australian faces a major problem with periodic shortages of blood components. These shortages are expected to become more frequent and severe as demand continues to increase at a faster rate than supply. Given that only five percent of the population is registered as blood donors, clearly, the challenge for the ARCBTS is to encourage more people to become regular blood donors. The current study was undertaken to assist the ARCBTS in achieving this goal, by identifying and investigating the factors that influence people's willingness to donate blood. Based on the findings of a literature review and focus groups, a conceptual model of "willingness to donate blood" was developed. The model included personal values, knowledge about blood donation, perceived risks associated with donating blood, and attitudes towards blood donation, as antecedents to willingness to donate.

The purpose of this study was to determine the knowledge of college students with regard to blood donation in order to be able to create an education program to recruit new donors. After creating a table of specifications, a questionnaire was designed and reviewed by a jury of experts, and then tested in a pilot study. In the final study, 782 usable questionnaires were completed; the majority of students from the convenience sample were female (60.9%), under the age of 21 (93.1%), white (86.2%), non-Hispanic (95.8%), and had earned some college credits (61.4%).The data were analyzed using mean, t-tests, and ANOVA to test five null hypotheses. The overall knowledge (60%) of the subjects was less (M = 13.11 out of a possible 22) than anticipated. Statistically significant differences in knowledge of blood donation was found between college males and females (p < 0 .028), among students by education level (p < 0.047), and among students who were frequent, occasional, and nondonors (p < 0.000). No difference was found in the knowledge of blood donation among students by age.
Department of Physiology and Health Science

Objective: To describe and compare the characteristics of blood donors and non-donors and to examine factors associated with donation, including motivators and barriers of blood donation in Guangzhou, China. Design: Cross-sectional survey using self-administered standardized structured questionnaires on both donors and non-donors. Setting: 12 mobile and 4 permanent blood donation stations in Guangzhou during the whole operation time. Participants: 500 blood donors who donated at the donation sites and 500 non-donors who never donated and passed by the station were asked to complete a self-administered questionnaire during Dec 10, 2013 to Jun 25, 2014. Main outcome measures: Blood donation or no donation. Results: 1080 questionnaires were collected, of which 1034(95.7%) questionnaires were valid. 602(58.2%) participants were donors and 432(41.8%) were non-donors. Older people (OR: 1.46, 95% confidence interval: 1.24 to 1.72, p<0.01), males (1.33, CI: 1.02 to 1.71, p=0.03), non-college-students (1.76, CI: 1.16 to 2.56, p<0.01) and people with higher education level (1.27, CI: 1.11 to 1.45, p<0.01) were more likely to be donors. The main objective of blood donation was helping patients (n=405, 68.2%), and the main reason of not donating was being in poor health (n=138, 33.1%). However, other motives, such as benefiting health and free check for blood type and body, and obstacles, such as failing to meet the requirements and fear, were also important. More male donors would donate again than females (80.5% vs. 68.5%, p<0.01), whereas more female donors showed uncertainty than males (25.9% vs. 16.6%, p<0.01). Usage of blood (n=182, 46.7%) was what non-donors wanted to know the most if they were to donate in the future. The majority of participants (n=730, 71.3%) considered raising the awareness of blood donation among people was one of the most effective ways of blood donation promotion. Television was considered as one of the most effective methods of blood donation promotion and recruitment, and was more acceptable to females. Younger participants preferred the internet. Conclusion: These findings suggest that raising the awareness of blood donation is vital. Campaigns should focus on multiple aspects targeting different groups of people. Television and the internet are useful tools of blood donation promotion and recruitment.
published_or_final_version
Public Health
Master
Master of Public Health

Collecting blood from repeat blood donors is cost effective and safer compared to other donor types. At the end of 2012, 84% of the SANBS donor panel were inactive or lapsed. There is a lack of research available on lapsed donors in the South African context and available research is mostly quantitative with subtle contradictions. Donors who donated blood in 2012 at fixed site donor centres in Port Elizabeth, and did not return in 2013, were studied. A descriptive analysis was done and a random sample of 78 lapsed donors were selected to participate in a face-to-face interview. Interviews were digitally recorded and transcribed. A grounded model was developed from various existing theories to seek out and conceptualise social patterns and structures of lapsed blood donors through a process of comparison. There were 10 062 donors who donated blood in 2012 and 4 923 became lapsed during 2013. Analysis of sub groups showed a higher proportion of donors who became lapsed in the following sub-categories: new donors (95%), re-joined donors (64%), black donors (63%), donors younger than 40 (61%), female donors (52%). The feedback received from the 11 participants highlighted peer pressure as the biggest motivator. Of the six communication theories applied, The Social Penetration Theory highlighted the cost-minus-benefit ratio which played a big role in a donor’s motivation and decision to return. The AIDA Marketing Model application described lapsed donor behaviour most comprehensively and it highlighted a missing step which was created as part of a Grounded Model and is called the AIDAA Model. The role and existence of peer pressure is directly linked to donor motivation and is categorised as an Action Motivator in the AIDAA Model. The new model creates additional recruitment opportunities which has not been explored and applied strategically before.

Dried blood spots is an alternative method to collect blood samples from research subjects. However, little is known about how hemoglobin and hematocrit affect bead-based multiplex assay performance. The purpose of this study was to determine how bead-based multiplex assays perform when analyzing dried blood spot samples. A series of four experiments outline the study each with a specific purpose. A total of 167 subject samples were collected and 92 different biomarkers were measured. Median fluorescence intensity results show a positive correlation between filtered and non-filtered samples. Utilizing a smaller quantity of sample results in a positive correlation to a larger sample. Removal of hemoglobin from the dried blood spot sample does not increase detection or concentration of biomarkers. Of the 92 different biomarkers measured 56 were detectable in 100-75% of the attempted samples. We conclude that blood biomarkers can be detected using bead-based multiplex assays. In addition, it is possible to utilize a smaller quantity of sample while avoiding the use of the entire sample, and maintaining a correlation to the total sample. While our method of hemoglobin was efficient it also removed the biomarkers we wished to analyze. Thus, an alternative method is necessary to determine if removing hemoglobin increases concentration of biomarkers. More research is necessary to determine if the biomarkers measured in this study can be measured over time or within an experimental model.

Thesis (MTech (Biomedical Technology))--Cape Peninsula University of Technology, 2016.
Biochemical and haematological changes occur in red blood cellular products during the recommended storage period of 35 to 42 days at 1°C to 6°C. The restriction of the sodium/potassium pump at specified temperatures result in low intracellular potassium ion levels while an increase in sodium ion levels are observed and acidosis occurs as a result of low pH concentrations due to glucose consumption. Structural and morphological changes occur such as the release of free haemoglobin, lactate dehydrogenase and potassium into the supernatant causing the formation of spheroechinocytes and osmotic fragility. All these factors negatively impact the rheological properties of blood. These changes that transpire in the red cells during the storage period are referred to as “storage lesions”. Transfusion-associated graft versus host disease is an immunological and often fatal adverse transfusion reaction with gamma irradiation of cellular blood products used as a preventative measure. Gamma irradiation exacerbates storage lesions and of particular concern has been the increased potassium levels resulting in neonatal and infant hyperkalaemia. The storage lesions occurring in non-irradiated red blood cellular products are well documented although the literature regarding its irradiated counterparts has been less studied. A study of this nature has not previously been done in Cape Town, South Africa.

Orientador: Regina Kiomi Takahira
Banca: Luiz Henrique de Araújo Machado
Banca: Simone Gonçalves Rodrigues Gomes
Resumo: O fator VIII (FVIII), o fator de von Willebrand (FvW) e o fibrinogênio são de suma importância na coagulação sanguínea, com diferentes funções fisiológicas. Por conter altas concentrações destes fatores a transfusão de crioprecipitado é uma terapia utilizada principalmente em pacientes que apresentam Doença de von Willebrand, Hemofilia A (deficiência do FVIII), ou pacientes que sofrem de hipo ou disfibrinogenemia. Este hemocomponente é um precipitado obtido após o descongelamento parcial (entre 1 e 6°C) do plasma fresco congelado, e também é conhecido como fator anti-hemofílico. Estudos têm demonstrado que o protocolo de congelamento e armazenamento do crioprecipitado afeta a qualidade do produto e a viabilidade destes fatores. Com o objetivo de avaliar a viabilidade do crioprecipitado canino em diferentes protocolos de congelamento e armazenamento foram avaliados dois grupos compostos de 10 unidades de crioprecipitado canino (n=20). Após a centrifugação das bolsas de sangue, o plasma fresco foi congelado a -80ºC (grupo I) e a -20ºC (grupo II). Vinte e quatro horas após o congelamento das bolsas, estas foram submetidas ao procedimento de extração do crioprecipitado. Os crioprecipitados das bolsas dos dois grupos foram submetidos à determinação do TP, TTPA, FVIII, FvW e fibrinogênio, no momento zero e após seis meses de estocagem. Para a realização das coletas, foram utilizadas bolsas sanguíneas triplas de plástico, com anticoagulante CPDA-1, sendo a bolsa principal com capacidade para 450 mL de sangue total (JP Indústria Farmacêutica®). Após o crioprecipitado devidamente pronto, uma alíquota de aproximadamente 5 mL da bolsa de crioprecipitado foi separada em criotubos para análise da amostra pré-estocagem e seis meses pós estocagem. As amostras obtidas em cada momento foram congeladas à -80ºC até o momento do processamento. Os resultados mostraram um decréscimo significativo dos fatores e ...
Abstract: The factor VIII (FVIII), the von Willebrand factor (vWF) and the fibrinogen are extremely important to the blood clotting process, with various physiological functions. Because it contains high concentrations of these factors and fibrinogen, transfusing cryoprecipitate is a therapy mainly used in patients who have von Willebrand disease, Hemophilia A (FVIII deficiency), or who suffer from hypo/dysfibrinogenemia. This hemocomponent is a precipitate obtained after the partial thawing process (between 1 and 6ºC) of fresh frozen plasma, and which is also known as the anti-hemophilic factor. Studies have demonstrated that the cryoprecipitate freezing and storage protocol affects the product quality as well as these factors viability. In order to evaluate the canine cryoprecipitate viability in different freezing and storage protocols, two groups containing 10 units of canine cryoprecipitate (n=20) were evaluated. Following the blood centrifugation, the fresh plasma was frozen at -80ºC (group I) and at -20ºC (group II). Twenty-four hours after freezing the blood bags, they were submitted to the cryoprecipitate extraction procedure. The cryoprecipitate from both groups of blood bags were submitted to the TP, TTPA, FVIII, FvW and fibrinogen determination process, at time zero and after six months of storage. During the collections, triple plastic blood bags were used, along with the anticoagulant CPDA-1, being the main bag capacity of 450 mL of whole blood (JP Indústria Farmacêutica®). After having the cryoprecipitate properly ready, an approximately 5 mL aliquot of cryoprecipitate was separated into cryovials to be analysed pre-storage and six months after storage. However, there was no significant difference between treatments, demonstrating that the difference in initial freezing temperature did not influence the decrease of the factors after six months storage at -20°C
Mestre

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
O fator VIII (FVIII), o fator de von Willebrand (FvW) e o fibrinogênio são de suma importância na coagulação sanguínea, com diferentes funções fisiológicas. Por conter altas concentrações destes fatores a transfusão de crioprecipitado é uma terapia utilizada principalmente em pacientes que apresentam Doença de von Willebrand, Hemofilia A (deficiência do FVIII), ou pacientes que sofrem de hipo ou disfibrinogenemia. Este hemocomponente é um precipitado obtido após o descongelamento parcial (entre 1 e 6°C) do plasma fresco congelado, e também é conhecido como fator anti-hemofílico. Estudos têm demonstrado que o protocolo de congelamento e armazenamento do crioprecipitado afeta a qualidade do produto e a viabilidade destes fatores. Com o objetivo de avaliar a viabilidade do crioprecipitado canino em diferentes protocolos de congelamento e armazenamento foram avaliados dois grupos compostos de 10 unidades de crioprecipitado canino (n=20). Após a centrifugação das bolsas de sangue, o plasma fresco foi congelado a -80ºC (grupo I) e a -20ºC (grupo II). Vinte e quatro horas após o congelamento das bolsas, estas foram submetidas ao procedimento de extração do crioprecipitado. Os crioprecipitados das bolsas dos dois grupos foram submetidos à determinação do TP, TTPA, FVIII, FvW e fibrinogênio, no momento zero e após seis meses de estocagem. Para a realização das coletas, foram utilizadas bolsas sanguíneas triplas de plástico, com anticoagulante CPDA-1, sendo a bolsa principal com capacidade para 450 mL de sangue total (JP Indústria Farmacêutica®). Após o crioprecipitado devidamente pronto, uma alíquota de aproximadamente 5 mL da bolsa de crioprecipitado foi separada em criotubos para análise da amostra pré-estocagem e seis meses pós estocagem. As amostras obtidas em cada momento foram congeladas à -80ºC até o momento do processamento. Os resultados mostraram um decréscimo significativo dos fatores e ...
The factor VIII (FVIII), the von Willebrand factor (vWF) and the fibrinogen are extremely important to the blood clotting process, with various physiological functions. Because it contains high concentrations of these factors and fibrinogen, transfusing cryoprecipitate is a therapy mainly used in patients who have von Willebrand disease, Hemophilia A (FVIII deficiency), or who suffer from hypo/dysfibrinogenemia. This hemocomponent is a precipitate obtained after the partial thawing process (between 1 and 6ºC) of fresh frozen plasma, and which is also known as the anti-hemophilic factor. Studies have demonstrated that the cryoprecipitate freezing and storage protocol affects the product quality as well as these factors viability. In order to evaluate the canine cryoprecipitate viability in different freezing and storage protocols, two groups containing 10 units of canine cryoprecipitate (n=20) were evaluated. Following the blood centrifugation, the fresh plasma was frozen at -80ºC (group I) and at -20ºC (group II). Twenty-four hours after freezing the blood bags, they were submitted to the cryoprecipitate extraction procedure. The cryoprecipitate from both groups of blood bags were submitted to the TP, TTPA, FVIII, FvW and fibrinogen determination process, at time zero and after six months of storage. During the collections, triple plastic blood bags were used, along with the anticoagulant CPDA-1, being the main bag capacity of 450 mL of whole blood (JP Indústria Farmacêutica®). After having the cryoprecipitate properly ready, an approximately 5 mL aliquot of cryoprecipitate was separated into cryovials to be analysed pre-storage and six months after storage. However, there was no significant difference between treatments, demonstrating that the difference in initial freezing temperature did not influence the decrease of the factors after six months storage at -20°C
FAPESP: 2012/13677-6

The Papuan Official Collection is a unique colonial collection assembled between 1907 and 1938 by government officers of the Australian administration of the Territory of Papua. It represents the first instance in the world where a colonial government made ethnographic collecting a requisite duty of its field officers. This unusual turn of events came at the insistence of Papua's first and longest serving Lieutenant-Governor, J.H.P. Murray, who administered the colony for over three decades. The story of how Murray came to establish an official government collection, and its subsequent formation, interpretation, and display over several decades, provides a case study par excellence for examining the complex relationship between colonialism, collecting and anthropology, which emerged over the course of the twentieth century. This study explores the genesis and history of the Papuan Official Collection, and situates it within the wider rubric of Australian colonialism. It establishes Murray as one of the earliest colonial governors in the world to implement, and publically advocate for, anthropology as a tool for colonial administration. It charts the rise of colonial discourses that linked loss of culture to physical demise in Pacific populations, and documents its influence on Australian colonial policy. Its findings suggest that the protection, preservation and management of Indigenous cultural heritage should not be considered a sideline of Australian colonial policy in Papua, but rather one of its most defining features. Over the course of its lifespan the Papuan Official Collection has been displayed in four different museums providing an opportunity to examine how a fixed body of objects (the collection) moved across time and space, to be re-interpreted into different conceptual frameworks: as curios and antiquities; ethnographic artefacts; scientific specimens; artworks; and, finally, as historic objects. My institutional history of the POC cautions against the assumption that colonial collections were always used as uncontested propaganda, which metropolitan museums were content to display on behalf of the imperial mission. While the Murray administration in Papua was able to provide goods and information to the various museums which housed the Collection, each institution had its own competing agendas and the relationship was not always a smooth one.

Beginning with the understanding that several European discourses compete for the right to interpret the physical traces of past human cultures I have examined what seem to be the major of these in the European context. They are the discourses of the divine, namely paganism and early Christianity, and the discourses of the secular and rational, the principal of which are antiquarianism and archaeology. Since the mid-nineteenth century archaeology has secured for itself official recognition as the proper knowledge of the material past. Archaeology is now to be found practised in almost every part of the world. The transfer of the discourses of archaeology and art history from the West to the non- West has, not surprisingly, included the transfer of the conservation ethic. While the conservation ethic has attained a foothold at a government and elite level in the non- West it appears to have little constituency at a local and non-elite level. In Thailand I have looked at Buddhism and animism as systems of knowledge about the material past and have found beliefs and practices which honour the spiritual essence of ancient remains but rarely seek to conserve their material fabric. In Australia the European conception of Aboriginal heritage is implicated in a primitivist longing for a 'traditional', unchanging Aboriginal culture in which authenticity is partly equated within pastness. Archaeology established its primacy in Australia by mixing its discourse with the discourse of heritage. It now finds its position destabilized as Aborigines themselves borrow elements of the same discourse in a counter-appropriation of their 'archaeological' cultural property. The universality of the conservation ethic is manifestly spurious. The West, in its bid to domesticate the past of the Other World, allies itself with the non-Western state. The state draws upon the material past as a resource for nation-building, monumentalizing the past also in the interests of legitimizing present political arrangements. This alliance of interests is fundamentally anti-religious. Its programme of 'conserving' ancient sites cuts across local practices.

Collection of previously published articles
Includes Allozyme electrophoresis / B.J. Richardson, P.R. Baverstock and M. Adams (1986)
Includes bibliographies
2 v. :
Title page, contents and abstract only. The complete thesis in print form is available from the University Library.
Thesis (D. Sc.)--University of Adelaide, 1988

Thesis (MPH.) -- University of Limpopo, 2012
The objectives of the study: To determine the knowledge, attitudes and practices of black employees of The University of Limpopo (Turfloop campus) about blood donation. To determine the degree of willingness by blacks to donate as well as suggesting some solutions to improve the performance by the South African National Blood Services. Design: A descriptive study utilizing a self-reported questionnaire was carried out. Setting: The University of Limpopo Turfloop Campus in the Limpopo Province of South Africa. Material and Methods: A total of 138 employees participated in the study. With the aid of Predictive Analytics Software (PASW), 136 male and 101 female participants were randomly selected from the 40 University departments. The mean ages by gender was 36.91, ±10, 06 years for males, and 41.93, ±9, 35 years for females. The mean ages by donor status was 40.24, ±10, 15 years for donors and 37.94, ±9, 9 years for non-donors. Outcome measures: Subjects demographic variables were determined by the use of a pre-tested self-reported questionnaire, which covered personal characteristics such as gender, age, residence, number of household members, marital status, educational background, faculty or department of vi employment, as well as the monthly income bracket. Knowledge, attitudes and practices regarding blood donation were also assessed using the pre-tested self-developed questionnaire. The following information was included: previous practices, current and lifetime practices of blood donation. Results: There was a general lack of practice of blood donation despite the presence of average knowledge relating to matters of blood donation amongst the black employees of the University of Limpopo, (Turfloop campus). There was no difference between black females and males as far as blood donation was concerned. There was a reflection of similar practices of blood donation by both genders. Blood donation generally started at a very late age due to early lack of exposure, information and awareness. Conclusion: Findings from this study showed that the prevalence of non-donors amongst the blacks is high. The attitudes and practices of non-donors towards donation were generally less favourable, but it can be argued that a high percentage of these findings may likely change with the right interventions.

In this dissertation I provide an ethnographic account of the testimony of four expert witnesses who appeared before the Commission of Inquiry on the Blood System in Canada (the Krever Inquiry) as they described the production of scientific knowledge and the role that knowledge played in the struggle to protect the blood supply from being contaminated by AIDS during the early 1980's. In doing so, I bring together the experts' testimony with contemporary documents gathered by the Commission and interviews I conducted with participants in the proceedings. Using insights drawn from the disciplines of anthropology, sociology, and history, I explore what the witnesses' accounts reveal about their understandings of their professional world and its relationships with other worlds, especially that of public health policy making. The Krever Inquiry offered a valuable opportunity for carrying out such an investigation. It provided a site where science was not only used, it was talked about. The Inquiry invited those involved in the blood system in the early 1980's to reflect upon and explain the beliefs and actions which surrounded one of the worst public health disasters in Canadian history and it asked the witnesses how similar catastrophes could be avoided in the future. As a result, many of the issues addressed at the hearings reflect matters of current concern in public health and medicine. The Inquiry addressed difficult issues surrounding the nature of scientific knowledge and its application in health decision-making and policy formulation. This study, therefore, may be of interest to those dealing with the problems surrounding uncertainty and the management of public health crises. It may also be of interest to those dealing with conflicts rising out of the intersection of different worlds of experience and practice, as well as to those involved in the current initiatives to both make medical and public health institutions more proactive, and inclusive, and public health decision-making more transparent.

Thesis (M.S.)--Indiana University, 2007.
Title from screen (viewed on Apr. 27, 2007) Department of Pharmacology & Toxicology, Indiana University-Purdue University Indianapolis (IUPUI) Includes vita. Includes bibliographical references (leaves 71-84)

The research attempts to evaluate the maintaining of the chain of evidence as a process of effective collection, handling and preservation of evidence. The concept "chain of evidence" refers to the process of collecting, handling and preservation of evidence until its presentation in court, as part of the investigation process. Evidence is anything that tends logically to prove or disprove a fact at issue in a judicial case. Evidence essentially consists of oral evidence, documentary evidence and real evidence. The value of evidence cannot be underestimated as evidence can make or break a case. It is therefore important that evidence is correctly and properly collected, handled and preserved to establish a strong link between an individual and a specific act.
Police Practice
Thesis ((M. Tech. (Forensic Investigation) Police Practice))