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Author: Grafiati

Published: 11 March 2023

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1

Louzil, Jan, Jana Stikarova, Dana Provaznikova, Ingrid Hrachovinova, Tereza Fenclova, Jan Musil, Martin Radek, et al. "Diagnosing Czech Patients with Inherited Platelet Disorders." International Journal of Molecular Sciences 23, no. 22 (November 19, 2022): 14386. http://dx.doi.org/10.3390/ijms232214386.

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A single-center study was conducted on 120 patients with inherited disorders of primary hemostasis followed at our hematological center. These patients presented a variety of bleeding symptoms; however, they had no definitive diagnosis. Establishing a diagnosis has consequences for the investigation of probands in families and for treatment management; therefore, we aimed to improve the diagnosis rate in these patients by implementing advanced diagnostic methods. According to the accepted international guidelines at the time of study, we investigated platelet morphology, platelet function assay, light-transmission aggregometry, and flow cytometry. Using only these methods, we were unable to make a definitive diagnosis for most of our patients. However, next-generation sequencing (NGS), which was applied in 31 patients, allowed us to establish definitive diagnoses in six cases (variants in ANKRD26, ITGA2B, and F8) and helped us to identify suspected variants (NBEAL2, F2, BLOC1S6, AP3D1, GP1BB, ANO6, CD36, and ITGB3) and new suspected variants (GFI1B, FGA, GP1BA, and ITGA2B) in 11 patients. The role of NGS in patients with suspicious bleeding symptoms is growing and it changes the diagnostic algorithm. The greatest disadvantage of NGS, aside from the cost, is the occurrence of gene variants of uncertain significance.

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Rivera, Candido E., Prakash Vishnu, Gretchen Schaef Johns, Rajiv K. Pruthi, and Dong Chen. "Identification of a Novel Heterozygous Mutation (c.2213T>G;p.Leu738Arg) in Platelet Glycoprotein ITGB3 gene in a Patient with Glanzmann's Thrombasthenia." Blood 132, Supplement 1 (November 29, 2018): 1158. http://dx.doi.org/10.1182/blood-2018-99-117995.

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Abstract BACKGROUND: Glanzmann's thrombasthenia (GT) is an inherited platelet disorder (IPD) that leads to clinically significant bleeding. Platelets from patients with GT can show qualitative or quantitative defects of platelet membrane glycoprotein (GP) IIb/IIIa complex. Most GT are caused by autosomal recessive genetic defects in ITGA2B and ITGB3 (genes for GPIIb and GPIIIa, respectively) with rare cases showing an autosomal dominant pattern. Accurate diagnosis of GT requires a constellation of both phenotypic and genetic studies. Here we report a unique case of autosomal dominant GT resulted from thorough phenotypic and genetic studies. PATIENTS AND METHODS: A 19 year-old woman was recently evaluated for life-long history of easy bruising and severe menorrhagia that was only partially responsive to medroxyprogesterone. She has severe thrombocytopenia first recognized shortly after birth with a platelet count around 20x109/L. Platelet size was normal. She was presumed to have immune-mediated thrombocytopenia (ITP) since early infancy, but lacking responsiveness to the conventional treatments of ITP including immunomodulation, splenectomy and thrombopoietin receptor agonists. Family history was negative for a bleeding diathesis. Both von Willebrand factor antigen and activity were within normal range. Bone marrow aspirate and biopsy with associated chromosome studies were all normal. Platelet surface glycoprotein assessment by flow cytometry using antibodies to GP IIb, IIIa, Ia, Ib-a, VI, IX, and whole exome sequencing (WES) utilizing a predefined list of 53 clinically significant genes* related to genetically IPDs were performed. Due to thrombocytopenia platelet aggregation studies could not be performed. METHODS/RESULTS: Platelet surface expression of GPIIb (CD41) and GPIIIa (CD61) were markedly decreased suggestive of a variant of GT (Figure). WES performed with Illumina HiSeq 2500 sequencing system by using Agilent SureSelelct CRE kit V1 to capture and target the exonic regions showed presence of a heterozygous mutation in ITGB3 gene (c2213T>G; p.Leu738Arg). By in silico prediction modeling, this mutation is likely to be pathogenic and results in the substitution of hydrophobic leucine with hydrophilic arginine in the transmembrane domain of the β3 subunit of alpha IIb/beta 3 integrin (αIIbβ3), the platelet receptor that binds to fibrinogen. Interestingly, GPVI is also decreased which may be associated with GPIIb and GPIIIa deficiency or due to accelerated shedding since no GPVI mutation was identified. CONCLUSION: We describe a patient with GT associated with a novel heterozygous autosomal dominant mutation in ITGB3 gene with substitution of leucine with arginine (c2213T>G; p.Leu738Arg). This deletion caused a low expression of αIIbβ3 integrin on her platelets surface and severe thrombocytopenia. This case underscores the importance of an integrated phenotyping and genotyping approach to render a definitive diagnosis of an IPD. *Platelet exome gene list: ABCG5, ABCG8, ACBD5, ACTN1, ANKRD26, ANO6, AP3B1, BLOC1S3, BLOC1S6, CYCS, DTNBP1, ETV6, FLI1, FLNA, GATA1, GFI1B, GP1BA, GP1BB, GP6, GP9, HOXA11, HPS1, HPS3, HPS4, HPS5, HPS6, ITGA2B, ITGB3, LYST, MASTL, MPL, MYH9, NBEAL2, ORAI1, PSRX1, P2RY12, PLA2G4A, PRKACG, RASGRP2, RBM8A, RUNX1, STIM1, STX11, STXBP2, TBXA2R, TBXAS1, THPO, TUBB1, UNC13D, VIPAS39, VPS33B, VPS45, WAS Figure Figure. Disclosures No relevant conflicts of interest to declare.

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Bastida, Jose Maria, Mónica del Rey, Rocío Benito, Isabel Sanchez-Guiu, Susana Riesco, Maria Jesús Peñarrubia, Rosa Fisac, et al. "Design and Validate of Next-Generation Sequencing Panel for Inherited Platelet Disorders." Blood 124, no. 21 (December 6, 2014): 4210. http://dx.doi.org/10.1182/blood.v124.21.4210.4210.

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Abstract Introduction The inherited platelet disorders (IPD) are a heterogeneous group of rare diseases including quantitative and/or qualitative platelet defects. Classically, patients with IPD are first functionally tested to know the possible defect before sequencing a single or a few genes. Phenotipyc diagnostic of IPD often requires light transmission aggregometry, quantitative analysis of receptors by flow cytometry and fluorescence and electron microscopy. This diagnostic strategy is complex, poorly standardised and time consuming. In addition, the phenotype can seldom guide the singles candidates genes for conventional Sanger squencing. Therefore, many patients remain without a accurate diagnosis of their IPD. Next generation sequencing (NGS) enables the simultaneous analysis of large groups of candidate genes in IPD and may be useful for rapid genetic diagnosis. The aim of this study was to design and validate a NGS panel for IPD. Patients & Methods We describe a strategy for rapid genetic diagnosis of IPD with Illumina sequencing of 60 candidates genes previously associated with IPD (table1). The baits were designed to tile 400 kb of gDNA sequence corresponding to the exons and splice sites in all known transcripts of the candidate genes identified. The bait library was tested by enriching the candidate IPD genes from 50 ng DNA obtained and sequencing by Nextera Rapid Custom Enrichment system. Results were analysed by Variant Studio system and Sequencing Analysis Viewer. A total of 21 patients were studied. For the validation process, DNA samples of 9 unrelated patients with IPD and their mutation known were used: two patients with Glanzmann Thrombasthenia (ITGA2B, p.Ala989Thr, p.Val982Met and p.Glu538Stop; ITGB3, p.Leu222Pro and p.Tyr216Cys), one Hermansky-Pudlak Sd. (HPS1, p.Glu204 Stop), another with Bernard-Soulier Sd. (GPIX, p.Phe71Stop), a case of Congenital Amegakaryocytic Thrombocytopenia (MPL, p.Arg102Cys), and 2 patients with Chediak Higashi Sd. (LYST, p.Gly3725Arg and p.Cys258Arg). Once validated, the NGS panel was used for genetic diagnostic of 8 patients with suspected IPD. Results Eleven mutations, previously identified in another center by conventional sequencing, were detected by our panel NGS (100% success in the validation process). We then tested this strategy for patients with suspected of IPD without diagnosis: I. a 13 years old girl with agenesis of the corpus callosum, facial dysmorphia, renal agenesis and thrombocytopenia was diagnosed of Thrombocytopenia FLNA-related and Periventricular Nodular Heterotopia (PNHV)[mutation in the FLNA was detected (p.Thr1232Ile)]. II. A two years old patient with severe thrombocytopenia and recurrent infections was diagnosed of Wiskott-Aldrich Sd (WAS, p.Arg268Gly fs Stop40). III. A patient with deafness, macrothrombocytopenia, and Döhle bodies was diagnosed by MYH9 deletion (MYH9; p.Asp1925Thr fs Stop23). IV. Six members of a family (2 of them with symptoms of mucocutaneous bleeding, and macrothrombocytopenia), in which an insertion in NBAL2 (p.Gly1142Arg fs Stop49) gene was found. Therefore, Gray Platelet Sd was diagnosed. Moreover, one patient with aspirin-like syndrome showed a P2RY12 mutation (p.Val279Met). Finally, mother and son with mild Hemophilia A (F8; p.Gln2208Arg) were detected. Conclusions This NGS panel enables a rapid genetic diagnostic of IPD. The use of NGS-based strategy is a feasible tool for the diagnosis of IPD that could be added to the screening of these disorders. Five mutations have not previously been described in the literature. Table 1: Sixty candidates' genes previously associated with IPD: Inherited Platelet Disorders Genes = 60 Cytoskeletal Assembly and Structural Proteins GP1BA, GP1BB, GP5, A2M, GP9, VWF, ITGA2, ITGA2B, ITGB3, ABCA1, ANO6,FERMT3, ACTN1, MASTL Disorders of agonist platelet receptors P2RX1, P2RY1, P2RY12, TBXA2R, TBXAS1, ADRA2A, GP6, CD36 o GP4, DTNBP1 Disorders signal transduction GNAI3, GNAQ, GNAS, PLA2G7, PLCB2PTS, GGCX, DPAGT1, DHCR24 Disorders of platelet granules NBEAL2, GFI1B, PLAU, HPS1, HPS3, HPS4, HPS5, HPS6, LYST, MLPH, BLOC1S3, BLOC1S6, AP3B1, VIPAS39, VPS33B, RAB27A, MYO5A, USF1 Thrombocytopenias and syndromes WAS, MYH9, FLNA, FLI1, STIM1, HOXA11, ANKRD26, MPL, RBM8A RUNX1, GATA1 Disclosures No relevant conflicts of interest to declare.

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Lopes, Emerson Delano, Cláudio Lúcio Fernandes Amaral, and Adalberto Brito Novaes. "DESEMPENHO NO CAMPO DE MUDAS DE Eucalyptus urophylla, Eucalyptus camaldulensis E Corymbia citriodora PRODUZIDAS EM DIFERENTES RECIPIENTES." FLORESTA 44, no. 4 (December 31, 2014): 589. http://dx.doi.org/10.5380/rf.v44i4.26480.

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Avaliou-se a produção de mudas de Eucalyptus urophylla, E. camaldulensis e Corymbia citriodora em blocos prensados e em dois modelos de tubetes por meio da avaliação da sobrevivência e do crescimento inicial das mudas. A sobrevivência foi avaliada aos sessenta dias e o crescimento inicial em altura e diâmetro ao nível do solo ao décimo mês pós-plantio. Foi utilizado o delineamento em blocos casualizados com cinco repetições. Mudas de E. urophylla e E. camaldulensis produzidas nos blocos e nos dois modelos de tubetes e mudas de C. citriodora produzidas nos dois modelos de tubetes obtiveram altas taxas de sobrevivência. Já as mudas de C. citriodora produzidas nos blocos apresentaram a menor média de sobrevivência no estudo. As médias de crescimento inicial das mudas de E. urophylla produzidas nos blocos e tubetes de 50 cm3 foram superiores às encontradas para mudas produzidas em tubetes de 35 cm3. Para E. camaldulensis e C. citriodora, as médias de crescimento inicial foram semelhantes nos três recipientes. Recomenda-se a produção de mudas de E. urophylla em tubetes de 50 cm3 ou em blocos prensados. As mudas de E. camaldulensis podem ser produzidas nos três recipientes, e C. citriodora nos tubetes de 50 e 35 cm3.Palavras-chave: Qualidade de mudas; desempenho pós-plantio. AbstractPerformance in the field of Eucalyptus urophylla, Eucalyptus camaldulensis and Corymbia citriodora produced in different containers. We evaluated the production of Eucalyptus urophylla, E. camaldulensis and Corymbia citriodora in pressed blocks and two tubes models by the evaluation of survival and early growth of seedlings. We assessed survival at sixty days and initial growth in height and diameter at ground level to the tenth month after planting. We used a randomized block design with five replications. Seedlings of E. urophylla and E. camaldulensis produced in blocks, and in two tube models, and seedlings of C. citriodora produced in two tube models had high survival rates. On the other hand, seedlings of C. citriodora produced in blocks presented the lowest survival mean. The initial seedling growth mean of E. urophylla produced in blocks and tubes of 50 cm3 were higher than for seedlings grown in tubes of 35 cm3. In relation to E. camaldulensis and C. citriodora the average initial growth were similar in the three containers. Production of seedlings of E. urophylla is recommended in tubes of 50 cm3 or in compressed blocks, E. camaldulensis seedlings can be produced in three containers, and C. citriodora in tubes of 50 and 35 cm3.Keywords: Seedling quality; post-planting performance.

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De Araújo, Gabriel Rios, Maria Carolina Ricciardi Sbizera, Marcela Lucas De Lima, José Victor Pronievicz Barreto, Michele Monteiro Sudak, Manuela Venturelli Finco, Diego Fagner Michelassi de Souza, Daiene Locoman, Dienifer Kely De Oliveira Ribeiro, and Luiz Fernando Coelho Da Cunha Filho. "Avaliação da Palatabilidade e da Resistência de Diferentes Rodenticidas Disponíveis para Uso por Empresas Especializadas, em Aviários no Município de Rolândia, Paraná." UNICIÊNCIAS 21, no. 1 (August 24, 2017): 2. http://dx.doi.org/10.17921/1415-5141.2017v21n1p2-6.

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A presença de roedores em estabelecimentos gera importantes prejuízos financeiros, além de representar risco à saúde animal e humana, fazendo-se necessário o seu controle. O trabalho teve como objetivo testar produtos rodenticidas disponíveis para o uso profissional quanto à aceitação pelos roedores e a sua resistência às ações ambientais. Foram utilizados três rodenticidas anticoagulantes de dose única; Brodifacum Bloco Parafinado (Syngenta®); Difetialona Bloco Extrusado (Bayer®); e Bromadiolone Bloco Extrusado (De Sangosse®). Os rodenticidas foram acondicionados em porta-iscas distribuídos em quatro aviários de uma granja produtora de aves de corte, no município de Rolândia, PR. Semanalmente, foi anotada a quantidade consumida e a deterioração dos rodenticidas. Os resultados obtidos nas quatro semanas mostram que na situação testada, o Brodifacum representa 68,3% do total de blocos consumidos, o Difetialona 26,7% e o Bromadiolone 5%. Em relação à resistência às ações ambientais, o Bromadiolone teve 19 blocos afetados (23,2%), o Difetialona 30 blocos (36,6%) e o Brodifacum 33 blocos (40,2%), tanto o consumo quanto a resistência entre os blocos não foram significativas estatisticamente. Os dados evidenciam que a escolha do rodenticida tem influência direta na eficiência, devendo ser um quesito valorizado no planejamento das ações de controle de roedores.Palavras-chave: Controle de Roedores. Rodenticida. Aceitação de Rodenticida.AbstractThe presence of rodents in establishments generates significant financial losses, as well as it poses a risk to animals and human’s health, making its control necessary. The study aimed to test rodenticides products available for professional use regarding the rodents’ acceptance and their resistance to environmental actions. Three single dose anticoagulant rodenticides wereused; Brodifacum Block waxed (Syngenta™); Difethialone Block Extruded (Bayer™); and Bromadiolone Block Extruded (De Sangosse™). Rodenticides were placed in bait holders distributed in four aviaries producing broilers in Rolândia city, PR. Thee amount consumed and the deterioration of rodenticides were weekly noted. The results obtained in four weeks show that in the tested situation, Brodifacum represents 68.3% of total consumed blocks, Difethialone 26,7% and Bromadiolone 5%. For resistance to environmental actions, Bromadiolone had 19 blocks affected (23.2%), Difetialona 30 blocks (36.6%) and Brodifacum 33 blocks (40.2%), both consumption and the resistance amongthe blocks were not statistically significant. The data show that the choice of rodenticide directly influences the efficiency and should be a question valued in the planning of rodent control measures.Keywords: Rodent control. Rodenticide. Rodenticides Acceptance.

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Mulyo, Jangkung Handoyo. "REGIONAL TRAE BLOCS : A CASE STUDY OF THE WELFARE IMPACT OF THE EUROPEAN UNION ON INDIA AND KENYA." Agro Ekonomi 8, no. 2 (November 29, 2016): 17. http://dx.doi.org/10.22146/agroekonomi.16812.

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Some countries, including the big player in the world economy, the USA , believe that free trade liberalization based on principles of non discriminatory and multilateral bases as well as an open market will improve the welfare of many countries. However, other countries do not follow the idea of trade liberalization and hence respond by forming regional trading blocs. Therefore, the existence of such trading blocs will be examined, whether they are a 'building blocks' or a 'stumbling blocks, for sustaining the free trade liberalization. And hence, this paper focuses on three main parts: rationalization of the establishment of trading blocs; identification of the critical factors for the success of these blocs; and presentation of empirical evidence for the welfare implications of the trade diverting effects of the European Union through the analysis of two less developed countries, India and Kenya.

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Férence Matumuini, N. E., V. Arseine Mboko, T. Gilbert Zougou, Fernand Tendonkeng, I. Abdourhamane Toure, Jules Lemoufouet, Emile Miegoue, Mustapha Malem, Benoît Boukila, and Etienne Pamo. "Caractéristiques physiques et ingestion des blocs multinutritionnels (BMN) à base des feuilles d’Albizia lebbeck associés à la paille de Hyparrhenia diplandra chez la brebis Djallonké au sud-est du Gabon." International Journal of Biological and Chemical Sciences 14, no. 1 (April 3, 2020): 143–53. http://dx.doi.org/10.4314/ijbcs.v14i1.12.

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En saison sèche, les compléments alimentaires offerts aux petits ruminants contiennent généralement du son de blé ou du maïs, qui ne sont pas toujours disponibles. Or, les feuilles d’Albizia lebbeck, légumineuse couramment rencontrée dans la région de Franceville, pourrait constituer une alternative à l’utilisation des céréales dans des rations pour ruminants. C’est ainsi que, les caractéristiques physiques et l’ingestion des blocs multinutritionnels (BMN) à base des feuilles d’Albizia lebbeck associées à la paille d’Hyparrhenia diplandra chez la brebis Djallonké, ont été étudiés à Franceville au Gabon en 2017. Trois formules de blocs multinutritionnels à savoir BMN0 (bloc à base de son de blé), BMN50 (son de blé substitué à 50% par les feuilles d’Albizia lebbeck) et BMN100 (son de blé substitué à 100% par les feuilles d’Albizia lebbeck), ont été utilisées. Les caractéristiques physiques des blocs (cohésion, dureté et couleur) ont été évaluées après séchage pendant 21 jours. Un dispositif en carré latin (3x3) a permis d’évaluer l’appétibilité et l’ingestion des blocs multinutritionnels associés à la paille d’Hyparrhenia diplandra chez les brebis Djallonké en saison sèche. Les résultats ont montré que la cohésion s’est dégradée avec le niveau croissant d’inclusion des feuilles d’Albizia lebbeck ; tandis que la dureté était moyenne pour le BMN50 et le BMN100. Les ingestions des BMN étaient de 133,72±27,35 ; 200,90±61,46 et 235,96±106,48 gMS/j/animal, correspondant à des niveaux d’appétibilité de 24,8±2,70 34,17±4,45 et 46,89±8,16%, respectivement pour les blocs BMN0, BMN50 et BMN100. Cette étude a révélé aussi que, la consommation des blocs avec inclusion des feuilles d’Albizia lebbeck a augmenté significativement l’ingestion de la paille d’Hyparrhenia diplandra. Aussi, cette légumineuse pourrait constituer une alternative à l’utilisation du son de blé dans les blocs à nutriments multiples en saison sèche. © 2020 International Formulae Group. All rights reserved. Mots clés: Alimentation, appétibilité, Franceville, légumineuses, petits ruminants English Title: Physical characteristics and intake of multinutrient blocks (BMN) based on Albizia lebbeck leaves associated with Hyparrhenia diplandra’s straw in Djallonké ewes in southeastern of Gabon English Abstract In the dry season, feed supplements for small ruminants usually contain wheat bran or corn, which are not always available. However, the leaves of Albizia lebbeck, legume commonly found in the region of Franceville, could be an alternative to the use of cereals in ruminant rations. Thus, the physical characteristics and intake of Albizia lebbeck leaves based multinutrient blocks (BMN) as supplement to Hyparrhenia diplandra’s straw in Djallonké ewes, were studied at Franceville in 2017. Three types of multinutrient blocks namely BMN0 (block of wheat bran without Albizia lebbeck leaves), BMN50 (wheat bran substituted 50% by Albizia lebbeck leaves) and BMN100 (wheat bran substituted 100% by Albizia lebbeck leaves), were used for this study. The physical characteristics (cohesion, hardness and color) of blocks were evaluated after drying in the shade during 21 days in a well-ventilated area. A Latin square arrangement (3x3) was used to assess the palatability and intake of multinutrient blocks associated with Hyparrhenia diplandra straw for Djallonké ewes at dry season. The results showed that the blocks cohesions of blocks degraded with the increasing level of inclusion of Albizia lebbeck leaves; while hardness was medium for BMN50 and BMN100. Intake of BMN was 133.72 ± 27.35; 200.90 ± 61.46 and 235.96 ± 106.48 gDM/d/animal, corresponding to a palatability of 24.8±2.70, 34.17±4.45 and 46.89±8.16%, respectively for BMN0, BMN50 and BMN100. This study also revealed that consumption of blocks with inclusion of Albizia lebbeck leaves significantly increased Hyparrhenia diplandra’s straw intake. Also, this legume could be an alternative to using wheat bran in multinutrient blocks at dry season. © 2020 International Formulae Group. All rights reserved. Keywords: Feeding, Franceville, legumes, palatability, small ruminants

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Raut, Sandesh S., and Abhijit Warudkar. "Pervious Concrete Paver Blocks." Journal of Advances and Scholarly Researches in Allied Education 15, no. 2 (April 1, 2018): 506–8. http://dx.doi.org/10.29070/15/56878.

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Andraus, Michel De Paula, Aline Assis Cardoso, Eva De Melo Ferreira, and Eliana Paula Fernandes Brasil. "PRODUÇÃO DE MUDAS FLORESTAIS INOCULADAS COM RIZÓBIOS E FUNGOS MICORRÍZICOS EM BLOCOS DE RESÍDUOS AGROINDUSTRIAIS." Nativa 8, no. 2 (April 10, 2020): 269. http://dx.doi.org/10.31413/nativa.v8i2.7843.

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O objetivo deste trabalho foi avaliar os aspectos fitotécnicos de mudas florestais cultivadas em blocos prensados com resíduos agroindustriais e o efeito da inoculação microbiológica sobre as mudas. Confeccionou-se quatro compostos com diferentes proporções de bagaço de cana, torta de filtro, fibra de coco e resíduos aviários. Nos blocos prensados do substrato selecionado foram plantadas sementes de Sesbania virgata, Eucalyptus grandi e Acacia mangium. As sementes de Sesbania virgata e Acacia mangium foram inoculadas com rizóbios e fungos micorrízicos específicos, as de Eucalyptus grandi foram inoculadas apenas com os fungos. Aos 70 dias após a semeadura, foram avaliados parâmetros de colonização micorrízica, nodulação, fitotécnicos e nutrição. O delineamento utilizado foi o de blocos completos casualizados em esquema fatorial 4 x 2. Os dados foram submetidos à análise de variância e teste de comparação de médias. Verificou-se que o sistema de produção de mudas em blocos prensados, confeccionados com resíduos agroindustriais, proporciona melhor desenvolvimento das mudas de Sesbania virgata em relação ao tubete convencional, porém não teve efeito no desenvolvimento de mudas de Acacia mangium em relação ao tubete convencional. A inoculação com rizóbio, isoladamente ou associada com fungos micorrízicos arbusculares na fase de produção de mudas, auxiliou no desenvolvimento da espécie Acacia mangium. A produção de Eucalyptus grandis em blocos prensados de resíduos agroindustriais não proporcionou vantagens sobre o desenvolvimento das mudas em tubetes.Palavras-chave: aproveitamento de resíduos; microrganismos benéficos; produção sustentável. PRODUCTION OF INOCULATED FOREST SHAPES WITH RHIZOBIUM AND MYCORRYZIC FUNGI IN AGRICULTURAL WASTE BLOCKS ABSTRACT: The objective of this work was to evaluate the phytotechnical aspects of plant seedlings in blocks pressed with agroindustrial residues and the effect of the microbiological inoculation on the seedlings. Four doses with different ratios of sugarcane bagasse, filter cake, coconut fiber and avian residues were made. In the pressed blocks of the selected substrate were planted seeds of Sesbania virgata, Eucalyptus grandi and Acacia Mangium. The seeds of Sesbania virgata and Acacia mangium were inoculated with rhizobia and specific mycorrhizal fungi. At 70 days after sowing, mycorrhizal colonization, nodulation, phytotechnical and nutrition parameters were evaluated. The design used was a randomized complete block in a 4 x 2 factorial scheme. It was verified that the system of production of seedlings in pressed blocks, made with agroindustrial residues provides better development of the seedlings of Sesbania virgata in relation to the conventional tube, but did not have effect in the development of seedlings of Acacia mangium in relation to the conventional tube. Inoculation with rhizobia alone or associated with arbuscular mycorrhizal fungi in the seedling production phase assisted the development of the Acacia mangium species. The production of Eucalyptus grandis in pressed blocks of agroindustrial residues did not provide advantages for the development of seedlings in tubes.Keywords: waste use; beneficial microorganisms; sustainable production.

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Barros, Enicléia Nunes de Sousa, and Saymon Martin Boaventura. "ESTUDO EXPERIMENTAL DO DESEMPENHO DE PAVIMENTOS PERMEÁVEIS COMO ALTERNATIVA DE REDUÇÃO DO ESCOAMENTO SUPERFICIAL EM ÁREAS URBANAS." REEC - Revista Eletrônica de Engenharia Civil 15, no. 2 (June 5, 2020): 300–313. http://dx.doi.org/10.5216/reec.v15i2.61831.

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RESUMO: O processo de urbanização das cidades sem o adequado planejamento de uso do solo provoca uma crescente impermeabilização deste, levando a constantes cheias nos centros urbanos. Entende-se que os sistemas tradicionais de drenagem resolvem apenas parte do problema, pois não agem nas causas mas somente nos efeitos. Nesse sentido o presente trabalho tem como objetivo avaliar o desempenho do pavimento permeável em relação à capacidade de infiltração das águas pluviais, visando à redução do escoamento superficial em áreas urbanas; para isso foi realizada a simulação chuvas para obter o escoamento por meio da equação de precipitação de Palmas-TO, onde foi possível aferir parâmetros de infiltração e escoamento; também foram produzidos e ensaiados blocos em concreto poroso para avaliar sua capacidade de infiltração. Na simulação efetuada sobre o pavimento permeável em blocos intertravados observou-se que ocorreu uma absorção da precipitação nos 9 minutos iniciais; já na simulação realizada sobre os blocos vazados percebeu-se que neste praticamente não ocorreu escoamento superficial. Os blocos em concreto poroso demonstraram uma ótima capacidade de infiltração, conseguindo comportar em seu interior 7,2 litros, o que significa uma infiltração instantânea de 7,2mm de chuva. ABSTRACT: The process of urbanization of cities without the adequate planning of land use causes a growing waterproofing of this, leading to constant floods in urban centers. It is understood that traditional drainage systems solve only part of the problem, as they do not act on causes but only on the effects. In this sense, the present work aims to evaluate the performance of the permeable pavement in relation to the infiltration capacity of rainwater, aiming at reducing surface runoff in urban areas; for this, the simulation was performed rains to obtain the flow through the Palmas-TO precipitation equation, where it was possible to measure infiltration and flow parameters; blocks were also produced and tested in porous concrete to assess their ability to infiltrate. In the simulation carried out on the permeable pavement in interlocked blocks it was observed that precipitation was absorbed in the initial 9 minutes; already in the simulation carried out on the leaked blocks it was noticed that in this practically no surface runoff occurred. The porous concrete blocks demonstrated an excellent infiltration capacity, managing to accommodate 7.2 liters inside, which means an instant infiltration of 7.2mm of rain.

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Hunya, Gábor. "Marathon mit Stolpersteinen." osteuropa 69, no. 6-8 (2019): 129–41. http://dx.doi.org/10.35998/oe-2019-0056.

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Niznikiewicz, Margaret. "The building blocks of social communication." Advances in Cognitive Psychology 9, no. 4 (December 31, 2013): 173–83. http://dx.doi.org/10.5709/acp-0145-6.

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Wani, Mohammad Vekas, and Mr Chitranjan Kumar. "Behaviour of Interlocking Hollow Concrete Blocks." International Journal of Trend in Scientific Research and Development Volume-2, Issue-4 (June 30, 2018): 2850–82. http://dx.doi.org/10.31142/ijtsrd14173.

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Wani, Mohammad Vekas, and Mr Chitranjan Kumar. "Behaviour of Interlocking Hollow Concrete Blocks." International Journal of Trend in Scientific Research and Development Volume-2, Issue-5 (August 31, 2018): 1853–63. http://dx.doi.org/10.31142/ijtsrd18203.

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Mukherjee, Anurag, and Suhrit Ghosh. "Core-Substituted Naphthalene-Diimides (cNDI) and Related Derivatives: Versatile Scaffold for Supramolecular Assembly and Functional Materials." Organic Materials 03, no. 03 (June 16, 2021): 281–92. http://dx.doi.org/10.1055/a-1530-0476.

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Naphthalene-diimide (NDI)-derived building blocks have been explored extensively for supramolecular assembly as they exhibit attractive photophysical properties, suitable for applications in organic optoelectronics. Core-substituted derivatives of the NDI chromophore (cNDI) differ significantly from the parent NDI dye in terms of optical and redox properties. Adequate molecular engineering opportunities and substitution-dependent tunable optoelectronic properties make cNDI derivatives highly promising candidates for supramolecular assembly and functional materials. This short review discusses recent development in the area of functional supramolecular assemblies based on cNDIs and related molecules.Table of contents1. Introduction2. General Supramolecular Assemblies of cNDI Derivatives3. cNDI-Based Chiral Supramolecular Assemblies and Functional Materials4. Controlled Supramolecular Polymerization with cNDI Derivatives5. Dimeric Naphthalimide-Based Building Blocks6. Conclusions and Outlook

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Ingole, Aniket Ravindra, Abdul Siddique Abdul Khalik Shaikh, and Rahul Ashokrao Tapke. "Environmental - Friendly Brick Blocks using E-Waste." International Journal of Trend in Scientific Research and Development Volume-2, Issue-2 (February 28, 2018): 1297–301. http://dx.doi.org/10.31142/ijtsrd9687.

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Álvarez Alonso, Marina, and Olga Río Suárez. "Elementos prefabricados de hormigón con cenizas volantes." Materiales de Construcción 41, no. 224 (December 30, 1991): 43–54. http://dx.doi.org/10.3989/mc.1991.v41.i224.730.

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Bahodirovich, Hojiyev Dilmurodjon, Muhammadjonov Akbarshoh Akramjon Og`Li Og`Li, Muzaffarova Dilshoda Botirjon Qizi, Ibrohimjonov Islombek Ilhomjon O`G`Li, and Ahmadjonova Musharrafxon Dilmurod Qizi. "About One Theorem Of 2x2 Jordan Blocks Matrix." American Journal of Applied sciences 03, no. 06 (June 12, 2021): 28–33. http://dx.doi.org/10.37547/tajas/volume03issue06-05.

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In this paper, we have studied one theorem on 2x2 Jordan blocks matrix. There are 4 important statements which is used for proving other theorems such as in the differensial equations. In proving these statements, we have used mathematic induction, norm of matrix, Taylor series of

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Etchevarne, Carlos, Maria Conceição Soares Meneses Lage, Benedito Batista Farias Filho, and Francisco Eroni Paz dos Santos. "CONTEXTOS TEMPORAIS EM SÍTIOS DE PINTURAS RUPESTRES, EM MORRO DO CHAPÉU, BAHIA." CLIO Arqueológica 35, no. 1 (June 1, 2020): 14. http://dx.doi.org/10.20891/clio.v35n1p14-38.

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A determinação de demarcadores cronológicos em sítios de pinturas rupestres tem sido um dos objetivos fundamentais dos arqueólogos que trabalham nesse campo da Arqueologia. Em Morro do Chapéu, Bahia, às características limitantes dos vestígios, impedindo a aplicação de métodos tradicionais de datação, soma-se, a escassa acumulação de solos, que resultam em pouca profundidade e parcos artefatos acumulados. O achado de blocos de pigmentos dentro de fogueiras datadas, no Sítio Toca da Figura, proporciona condições impares para proporcionar um ponto temporal, tanto procurado. O uso de métodos analíticos da área Arqueometria tornou possível um quadro de vinculações entre as figuras representadas no painel do abrigo e os blocos de pigmentos das fogueiras.TEMPORAL CONTEXTS IN ROCK PAINTINGS SITES IN MORRO DO CHAPEU, BAHIAABSTRACTThe definitions of chronological markers of painting on rock art in Archeological sites, has been one of fundamental aiming for all searchers that work in Archeology. In Morro do Chapéu-Ba, limited features of traces are the main reason that make difficult to use traditional methods. In addition, it worst with shallow depth soil, resulting scarce and poor artefacts. However, the finding of pigment blocks inside of campfire in the Toca da Figura, provides exceptional opportunity to determine when it was done. The use of analytical methods from Archeometry became possible to build a frame of links between pictures painted on the panel and the pigment blocks of campfire.Keywords: Rock painting; Chapada Diamantina / BA; Archeometry.

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Guo, Anhong, Ilter Canberk, Hannah Murphy, Andrés Monroy-Hernández, and Rajan Vaish. "Blocks." Proceedings of the ACM on Interactive, Mobile, Wearable and Ubiquitous Technologies 3, no. 3 (September 9, 2019): 1–24. http://dx.doi.org/10.1145/3351241.

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21

Summers, Anthony. "Regional nerve blocks 1: wrist blocks." Journal for Nurse Practitioners 13, no. 7 (July 2017): e328. http://dx.doi.org/10.1016/j.nurpra.2017.05.028.

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Summers, Anthony. "Regional nerve blocks 2: ankle blocks." Journal for Nurse Practitioners 13, no. 7 (July 2017): e328. http://dx.doi.org/10.1016/j.nurpra.2017.05.029.

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23

Giraldelli, Mariana Aparecida, Osvaldo Alves Pereira, Samuel Felipe Dos Santos, Mirela Aguiar Brasil, and Stefani Karoline Teodoro Pinheiro. "Técnica de alvenaria adobe: Reprodução do método construtivo com e sem o uso de fibra vegetal." UNICIÊNCIAS 25, no. 1 (June 14, 2021): 10–13. http://dx.doi.org/10.17921/1415-5141.2021v25n1p10-13.

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A técnica construtiva do adobe é um método de alvenaria em que se utilizam blocos em terra crua que possui como principal matéria prima o solo. De maneira geral, esta técnica proporcionou grandes feitos na historia da humanidade e reproduz com legitimidade um método construtivo simples, de fácil acesso e sustentável. Esta técnica bioconstrutiva é uma proposta de investimento para vários estudos, pois é um método que acompanhou a história da engenharia e da arquitetura e é possível ser aperfeiçoado podendo ser feita de várias formas possíveis com diversos agregados. Este trabalho apresenta a técnica do adobe que é uma alternativa sustentável para construções artesanais, assim como a experiência com a técnica e a mudança de comportamento para a confecção dos blocos com diferentes proporções de fibra vegetal em sua constituição, sendo que esta adição é normalmente feita para auxiliar na capacidade de resistência mecânica do bloco. Este trabalho consiste na mescla de uma revisão narrativa e a reprodução da confecção da técnica, sendo a primeira baseada em livros, artigos, seminários e demais obras cientifica que abordam o tema de construções em terra crua com destaque na técnica adobe, abordando os principais pontos para a confecção dos blocos e comparando os modos de confecção. Palavras-chaves: construção em terra crua; adobe; bloco de terra crua. Abstract The constructive technique of adobe is a masonry method in which blocks are used on raw earth whose main raw material is soil. In general, this technique provided great achievements in the history of humanity and legitimately reproduces a simple construction method, easily accessible and sustainable. This bioconstructive technique is an investment proposal for several studies, as it is a method that has followed the history of engineering and architecture and it is possible to be perfected and it can be done in several possible ways with different aggregates. This work presents the adobe technique, which is a sustainable alternative for artisanal constructions, as well as the experience with the technique and the change in behavior for the making of blocks with different proportions of vegetable fiber in their constitution, and this addition is usually made to assist in the mechanical strength of the block.This work consists of a mixture of a narrative review with empirical preparation, the first being based on books, articles, seminars and other scientific works that address the theme of constructions on raw earth with emphasis on the adobe technique, addressing the main points for the making of the blocks and drawing a comparison about this production. Keywords: raw earth construction; adobe; block of raw earth.

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De Isidro Gordejuela, F. "Estructuras de fábrica de ladrillo y bloque cerámico no armada." Informes de la Construcción 47, no. 440 (December 30, 1995): 33–40. http://dx.doi.org/10.3989/ic.1995.v47.i440.1046.

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25

Mas, J. M., and C. C. Kirschbaum. "Ensayos de resistencia a la compresión de bloques de suelo cemento." Informes de la Construcción 63, no. 524 (December 16, 2011): 43–48. http://dx.doi.org/10.3989/ic.10.048.

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26

SK, Jose. "Building Blocks Using Foamed Concrete with Industrial Waste Materials." Open Access Journal of Waste Management & Xenobiotics 4, no. 4 (2020): 1–11. http://dx.doi.org/10.23880/oajwx-16000167.

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Clay bricks or hollow/solid concrete blocks are used as infills for reinforced concrete framed structures in the present construction scenario. There is substantial depletion of natural resources during the production of conventional bricks, which create environmental pollution due to burning of bricks. Also, for the production of hollow/solid cement concrete blocks, large quantities of cement and natural aggregates are being used. This enforces researchers to develop a more feasible, lighter and greener alternate material for infills. Foamed concrete (FC) is such an innovative and versatile material, which consists of a cement based mortar having minimum 20% of volume filled with air. The effective consumption of industrial by-products for the production of FC lead to preservation of natural resources, solving disposal issues of these wastes. FC is found to be economically viable, light in weight, durable, thermally resistive as well as environmentally sustainable. This research focuses on the feasibility of utilizing the industrial waste materials such as fly ash and GGBFS as partial substitute for cement and quarry dust as substitute for fine aggregate. The influence of these waste materials on foamed concrete and the development of properties like compressive strength, dry density, water absorption and thermal conductivity were studied.

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Anosike, M. N., and A. A. Oyebade. "Sandcrete Blocks and Quality Management in Nigeria Building Industry." Journal of Engineering, Project, and Production Management 2, no. 1 (January 31, 2012): 37–46. http://dx.doi.org/10.32738/jeppm.201201.0005.

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28

RIBEIRO, A. A., C. L. MIOTO, R. MACHADO, M. L. ASSINE, E. GUARALDO, and A. C. PARANHOS. "Structural Blocks as Flood Control in Brazilian Pantanal." Anuário do Instituto de Geociências - UFRJ 41, no. 3 (December 4, 2018): 434–44. http://dx.doi.org/10.11137/2018_3_434_444.

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29

Kiefer, Alexander, and Uli Kazmaier. "Syntheses of Cyclomarins – Interesting Marine Natural Products with Distinct Mode of Action towards Malaria and Tuberculosis." Synthesis 51, no. 01 (November 7, 2018): 107–21. http://dx.doi.org/10.1055/s-0037-1610377.

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The cyclomarins are cyclic heptapeptides from marine streptomycetes containing four rather unusual amino acids. Interestingly, the cyclomarins address two completely different targets: ClpC1, a subunit of the caseinolytic protease of Mycobacterium tuberculosis (MTB), as well as PfAp3Aase of Plasmodium falciparum. Therefore, the cyclomarins are interesting lead structures for the development of drugs targeting tuberculosis and malaria. As a result, several synthetic protocols towards the synthesis of these unusual building blocks as well as the natural products themselves have been developed, which will be discussed in this review.1 Introduction2 Synthesis of the Building Blocks3 Total Synthesis of Cyclomarin C by Yao and Co-workers4 Total Synthesis of Cyclomarin A and C by Barbie and Kazmaier5 Conclusion

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Sousa, J. G. G., E. Bauer, and R. M. Sposto. "Empleo de residuos de la construcción civil como áridos reciclados. Producción de bloques de hormigón." Materiales de Construcción 53, no. 271-272 (December 30, 2003): 59–70. http://dx.doi.org/10.3989/mc.2003.v53.i271-272.289.

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31

高家斌, 高家斌, 簡惠閔 Chia-Pin Kao, 程筑鈺 Hui-Min Chien, and 蘇玲慧 Chu-Yu Cheng. "運用積木輔助影像敘事教育對幼兒創造力影響之研究." 數位學習科技期刊 14, no. 3 (July 2022): 093–120. http://dx.doi.org/10.53106/2071260x2022071403004.

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32

Su Yunzheng, 苏云征, 郝群 Hao Qun, 曹杰 Cao Jie, 闫雷 Yan Lei, and 武帅 Wu Shuai. "合并分割块的点云语义分割方法." Infrared and Laser Engineering 50, no. 10 (2021): 20200482. http://dx.doi.org/10.3788/irla20200482.

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33

Sebastian, Reena, and K. A. Germina K.A Germina. "On Square Sum Chain Graphs whose Blocks are Complete Graphs." International Journal of Scientific Research 3, no. 2 (June 1, 2012): 298–304. http://dx.doi.org/10.15373/22778179/feb2014/95.

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34

Gavhane, Bhagyashree Ashok, and Prashant Vitthalrao Kathole. "Alternative RAM Mapping Algorithm for Embedded Memory Blocks in FPGA." International Journal of Trend in Scientific Research and Development Volume-2, Issue-3 (April 30, 2018): 2542–49. http://dx.doi.org/10.31142/ijtsrd7172.

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35

Prissang, René Hellä, Tomas Lehtimäki, Pauli Saksa, Jorma Nummela, and Aimo Vuento. "Localisation of undisturbed blocks in larger dimension stone rock masses." Zeitschrift der Deutschen Gesellschaft für Geowissenschaften 158, no. 3 (September 1, 2007): 471–82. http://dx.doi.org/10.1127/1860-1804/2007/0158-0471.

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36

Eggener, Keith. "Building Blocks." Journal of the Society of Architectural Historians 79, no. 4 (December 1, 2020): 373–75. http://dx.doi.org/10.1525/jsah.2020.79.4.373.

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37

Adeney, Mark. "Study blocks." Nursing Standard 6, no. 3 (October 9, 1991): 51–52. http://dx.doi.org/10.7748/ns.6.3.51.s62.

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38

Gruszka, Sarah. "Blocus (Blokada)." Témoigner. Entre histoire et mémoire, no. 121 (October 1, 2015): 170–71. http://dx.doi.org/10.4000/temoigner.3469.

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39

Kennedy, Rachel. "Starting blocks." Nursing Standard 16, no. 9 (November 14, 2001): 28. http://dx.doi.org/10.7748/ns.16.9.28.s44.

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40

Shapiro, R. Benjamin, and Matthew Ahrens. "Beyond blocks." Communications of the ACM 59, no. 5 (April 26, 2016): 39–41. http://dx.doi.org/10.1145/2903751.

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41

Adams-Hamoda, Mary G., and Michele M. Pelter. "Heart Blocks." American Journal of Critical Care 12, no. 1 (January 1, 2003): 77–78. http://dx.doi.org/10.4037/ajcc2003.12.1.77.

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42

Greengrass, Roy A., and Reynold Duclas. "Paravertebral Blocks." International Anesthesiology Clinics 50, no. 1 (2012): 56–73. http://dx.doi.org/10.1097/aia.0b013e318216c03e.

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43

Rosenthal, Christina Karlsson. "Building blocks." Nature Reviews Molecular Cell Biology 9, S1 (December 1, 2008): s9. http://dx.doi.org/10.1038/nrm2583.

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44

Robinson, Hilary, and Vinod Hallan. "Stumbling blocks." Education 3-13 19, no. 3 (October 1991): 41–43. http://dx.doi.org/10.1080/03004279185200321.

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45

Holt, Gary A., Kay E. Holt, Ashish Chandra, and Richard Hood. "Uncarved Blocks." Journal of Pharmacy Teaching 6, no. 3 (May 11, 1998): 77–78. http://dx.doi.org/10.1300/j060v06n03_08.

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46

McCreery, Ryan. "Building Blocks." Hearing Journal 65, no. 12 (December 2012): 10. http://dx.doi.org/10.1097/01.hj.0000423569.01092.96.

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McCreery, Ryan. "Building Blocks." Hearing Journal 66, no. 1 (January 2013): 11. http://dx.doi.org/10.1097/01.hj.0000425771.34261.05.

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48

McCreery, Ryan. "Building Blocks." Hearing Journal 66, no. 2 (February 2013): 10. http://dx.doi.org/10.1097/01.hj.0000427118.33431.c6.

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49

McCreery, Ryan. "Building Blocks." Hearing Journal 66, no. 3 (March 2013): 14. http://dx.doi.org/10.1097/01.hj.0000427527.55840.9b.

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McCreery, Ryan. "Building Blocks." Hearing Journal 66, no. 4 (April 2013): 8–10. http://dx.doi.org/10.1097/01.hj.0000429415.23420.97.

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