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Anwar, Mahdalena Anwar, Siti Nurjanah Nurjanah, and Winiati P. Rahayu. "Aplikasi Basic Local Alignment Search Tool (BLAST) NCBI Pada Penelitian Molekuler Salmonella SPP." Syntax Literate ; Jurnal Ilmiah Indonesia 7, no. 11 (November 15, 2022): 15446. http://dx.doi.org/10.36418/syntax-literate.v7i11.9037.
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Basic Local Alignment Search Tool (BLAST) merupakan tool pada website NCBI yang banyak digunakan pada penelitian mikrobiologi molekuler. Tujuan penelitian ini adalah melakukan review sistematik dari fungsi program BLAST dan menentukan parameter penting yang diaplikasikan pada penelitian Salmonella asal produk pangan. Salmonella spp. menjadi fokus karena bakteri ini terdiri dari banyak serovar yang memiliki variasi dalam mekanisme virulensi, perbedaan patogenitas dan adaptasi Salmonella terhadap inangnya. Pencarian artikel dilakukan dengan menggunakan mesin pencari PUBMED dan Google Scholar, dengan penentuan kriteria artikel dengan menggunakan metode PICO (problem/population, intervention, comparation, outcome), dan penyeleksian artikel dengan menggunakan metode PRISMA. Hasil penelitian terseleksi 30 artikel yang masuk dalam kriteria. Fungsi BLAST diaplikasikan pada 4 fungsi yaitu: (1) mengidentifikasi sekuens, (2) menemukan DNA target dengan efisien, (3) menyimpulkan fungsi gen dan menduga domain architecture dari struktur proteinnya, serta (4) merancang primer. BLAST NCBI yang digunakan adalah BLASTn, BLASTp serta PRIMER BLAST. Aplikasi tersebut dapat digunakan dengan memperhatikan parameter penting pada empat fungsi tersebut
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Smith, Kyle D., Tao Chen, and Rong Z. Gan. "Hearing Damage Induced by Blast Overpressure at Mild TBI Level in a Chinchilla Model." Military Medicine 185, Supplement_1 (January 2020): 248–55. http://dx.doi.org/10.1093/milmed/usz309.
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Abstract Introduction The peripheral auditory system and various structures within the central auditory system are vulnerable to blast injuries, and even blast overpressure is at relatively mild traumatic brain injury (TBI) level. However, the extent of hearing loss in relation to blast number and time course of post-blast is not well understood. This study reports the progressive hearing damage measured in chinchillas after multiple blast exposures at mild TBI levels (103–138 kPa or 15–20 psi). Materials and Methods Sixteen animals (two controls) were exposed to two blasts and three blasts, respectively, in two groups with both ears plugged with foam earplugs to prevent the eardrum from rupturing. Auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) were measured in pre- and post-blasts. Immunohistochemical study of chinchilla brains were performed at the end of experiment. Results Results show that the ABR threshold and DPOAE level shifts in 2-blast animals were recovered after 7 days. In 3-blast animals, the ABR and DPOAE shifts remained at 26 and 23 dB, respectively after 14 days. Variation of auditory cortex damage between 2-blast and 3-blast was also observed in immunofluorescence images. Conclusions This study demonstrates that the number of blasts causing mild TBI critically affects hearing damage.
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Smith, Albert T. "Discrimination of explosions from simultaneous mining blasts." Bulletin of the Seismological Society of America 83, no. 1 (February 1, 1993): 160–79. http://dx.doi.org/10.1785/bssa0830010160.
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Abstract Large mining blasts can complicate the identification and discrimination of small underground nuclear explosions and may offer evasion opportunities. Mining blasts typically show a unique spectral signature: spectral reinforcements associated with time-delayed detonations between adjacent shot holes or rows of shots. Discrimination of a nuclear detonation that is simultaneous with a mining blast must depend upon recognizing significant spectral or waveform abnormalities within seismic signals from the mining blasts. In this investigation, large, simultaneous detonations within mining blasts are simulated for observed explosions from the Mesabi Range in Minnesota and for a series of quarry blasts at the Kaiser Permanente Quarry in Cupertino, California, which included a simultaneous detonation conducted by Lee et al. (1989). The Mesabi explosions are examples of large, ripple-fired blasts with known blast patterns (Smith, 1989). The models suggest that a large, single, deeply buried explosion dominates the waveform signature if it contains more than 5 to 15% of the total explosive in the mining blast. Spectral signatures of these combined explosions still show periodicities characteristic of ripple firing; however, their amplitude is greatly reduced. Inclusion of a deep simultaneous shot accentuates the high-frequency spectrum. If single explosions are sufficiently close to the combined quarry blast, their application as empirical Green's functions can isolate the simultaneous explosion within the blast. If empirical Green's functions are within 0.5 km of quarry blasts, individual explosions can be retrieved if delays are 100 msec between shot holes and signals extend to 40 Hz. Identification of large, simultaneous detonations within a blast may depend upon knowledge of the mine's blasting practices and its variability from blast to blast.
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Nara, N., and E. A. McCulloch. "Membranes replace irradiated blast cells as growth requirement for leukemic blast progenitors in suspension culture." Journal of Experimental Medicine 162, no. 5 (November 1, 1985): 1435–43. http://dx.doi.org/10.1084/jem.162.5.1435.
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The blast cells of acute myeloblastic leukemia (AML) may be considered as a renewal population, maintained by blast stem cells capable of both self-renewal and the generation of progeny with reduced or absent proliferative potential. Blast progenitor renewal is manifested in suspension culture by an exponential increase in clonogenic cells. This growth requires that two conditions be met: first, the cultures must contain growth factors in media conditioned either by phytohemagglutinin (PHA)-stimulated mononuclear leukocytes (PHA-LCM), or by cells of the continuous bladder carcinoma line HTB9 (HTB9-CM). Second, the cell density must be maintained at 10(6) blasts/ml; this may be achieved by adding irradiated cells to smaller numbers of intact blasts. We are concerned with the mechanism of the feeding function. We present evidence that (a) cell-cell contact is required. (b) Blasts are heterogeneous in respect to their capacity to support growth. (c) Fractions containing membranes from blast cells will substitute for intact cells in promoting the generation of new blast progenitors in culture. (d) This membrane function may be specific for AML blasts, since membranes from blasts of lymphoblastic leukemia or normal marrow cells were inactive.
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Morley, Michael G., Jackie K. Nguyen, Jeffrey S. Heier, Bradford J. Shingleton, Joseph F. Pasternak, and Kraig S. Bower. "Blast Eye Injuries: A Review for First Responders." Disaster Medicine and Public Health Preparedness 4, no. 2 (June 2010): 154–60. http://dx.doi.org/10.1001/dmp.v4n2.hra10003.
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ABSTRACTAs the rate of terrorism increases, it is important for health care providers to become familiar with the management of injuries inflicted by blasts and explosions. This article reviews the ocular injuries associated with explosive blasts, providing basic concepts with which to approach the blast-injured patient with eye trauma. We conducted a literature review of relevant articles indexed in PubMed between 1948 and 2007. Two hundred forty-four articles were reviewed. We concluded that ocular injury is a frequent cause of morbidity in blast victims, occurring in up to 28% of blast survivors. Secondary blast injuries, resulting from flying fragments and debris, cause the majority of eye injuries among blast victims. The most common blast eye injuries include corneal abrasions and foreign bodies, eyelid lacerations, open globe injuries, and intraocular foreign bodies. Injuries to the periorbital area can be a source of significant morbidity, and ocular blast injuries have the potential to result in severe vision loss.(Disaster Med Public Health Preparedness. 2010;4:154-160)
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Tan, X. Gary, and Peter Matic. "Simulation of Cumulative Exposure Statistics for Blast Pressure Transmission Into the Brain." Military Medicine 185, Supplement_1 (January 2020): 214–26. http://dx.doi.org/10.1093/milmed/usz308.
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Abstract Introduction This study develops and demonstrates an analysis approach to understand the statistics of cumulative pressure exposure of the brain to repetitive blasts events. Materials and Methods A finite element model of blast loading on the head was used for brain model biomechanical responses. The cumulative pressure exposure fraction (CPEF), ranging from 0.0 to 1.0, was used to characterize the extent and repetition of high pressures. Monte Carlo simulations were performed to generate repetitive blast cumulative exposures. Results The blast orientation effect is as influential as the blast overpressure magnitudes. A 75° (from the side) blast orientation can produce CPEF values exceeding traumatic brain injury pressure thresholds >0.95 while, for the same blast overpressure, a 0° (front) blast orientation results in a CPEF <0.25. Monte Carlo results for different sequences reflecting notional operational and training environments show that both mean values and standard deviations of CPEF reach the statistically equilibrium state at a finite value of n exposures for each sequence. Conclusions Statistical convergence of the brain pressure response metrics versus number of blasts for different exposures characterizes the transitions from “low” to “high” number of blasts and quantitatively highlights the differences between operational and training exposures.
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Fields, David M., Nils Olav Handegard, John Dalen, Christiane Eichner, Ketil Malde, Ørjan Karlsen, Anne Berit Skiftesvik, Caroline M. F. Durif, and Howard I. Browman. "Airgun blasts used in marine seismic surveys have limited effects on mortality, and no sublethal effects on behaviour or gene expression, in the copepod Calanus finmarchicus." ICES Journal of Marine Science 76, no. 7 (August 7, 2019): 2033–44. http://dx.doi.org/10.1093/icesjms/fsz126.
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Abstract Seismic surveys use airguns that emit low frequency high magnitude sound to detect subsea resources and to map seabed geology. The effect of seismic blasts on Calanus spp., a key food source for commercially important fish, was assessed in field experiments. Immediate mortality of copepods was significantly different from controls at distances of 5 m or less from the airguns. Mortality 1 week after the airgun blast was significantly higher—by 9% relative to controls—in the copepods placed 10 m from the airgun blast but was not significantly different from the controls at a distance of 20 m from the airgun blast. The increase in mortality—relative to controls—did not exceed 30% at any distance from the airgun blast. Only two genes changed in response to the airgun blast; however, their function is unknown. There were no sublethal effects of the seismic blasts on the escape performance or the sensory threshold needed to initiate an escape response at any of the distances from the airgun blast that were tested. Results from these experiments suggest that seismic blasts have limited effects on the mortality or escape response of Calanus sp. within 10 m of the blast and no measurable impact at greater distances.
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Iacono, Diego, Erin K. Murphy, Cheryl D. Stimpson, Fabio Leonessa, and Daniel P. Perl. "Double Blast Wave Primary Effect on Synaptic, Glymphatic, Myelin, Neuronal and Neurovascular Markers." Brain Sciences 13, no. 2 (February 8, 2023): 286. http://dx.doi.org/10.3390/brainsci13020286.
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Explosive blasts are associated with neurological consequences as a result of blast waves impact on the brain. Yet, the neuropathologic and molecular consequences due to blast waves vs. blunt-TBI are not fully understood. An explosive-driven blast-generating system was used to reproduce blast wave exposure and examine pathological and molecular changes generated by primary wave effects of blast exposure. We assessed if pre- and post-synaptic (synaptophysin, PSD-95, spinophilin, GAP-43), neuronal (NF-L), glymphatic (LYVE1, podoplanin), myelin (MBP), neurovascular (AQP4, S100β, PDGF) and genomic (DNA polymerase-β, RNA polymerase II) markers could be altered across different brain regions of double blast vs. sham animals. Twelve male rats exposed to two consecutive blasts were compared to 12 control/sham rats. Western blot, ELISA, and immunofluorescence analyses were performed across the frontal cortex, hippocampus, cerebellum, and brainstem. The results showed altered levels of AQP4, S100β, DNA-polymerase-β, PDGF, synaptophysin and PSD-95 in double blast vs. sham animals in most of the examined regions. These data indicate that blast-generated changes are preferentially associated with neurovascular, glymphatic, and DNA repair markers, especially in the brainstem. Moreover, these changes were not accompanied by behavioral changes and corroborate the hypothesis for which an asymptomatic altered status is caused by repeated blast exposures.
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Debnath, Jhuma, Ajay Kumar, and Hrishikesh Sharma. "Numerical investigation of Reinforced-concrete beam-column joints under contact and close-in blast application." Proceedings of the 12th Structural Engineering Convention, SEC 2022: Themes 1-2 1, no. 1 (December 19, 2022): 1203–8. http://dx.doi.org/10.38208/acp.v1.641.
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The behavior of the concrete and the steel material under blast loads are different. They have different mode of failures under blast loads. Also, responses differ according to the blast types concerning the proximity of the charge kept. It causes different failure modes in the structural members. Close-in or contact blast causes the spallation of concrete. In the near-field blasts, it causes bending failure in the structural members. The behavior of the mode of failure of various joint types subjected to contact-blast and close-in blast loads are numerically studied here. Three different joints simulated to carry on blast loads—exterior beam-column joint, interior beam-column joint, and knee joint simulated numerically under the close-in and contact loads. The charge for the contact blasts were applied to the joint is placed in contact with the joint core, and was not put at the beam or the column member of the joint cut section. In the current work, the failure behavior and the response of the RC beam-column joints is concluded.
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Ha, K., MH Freedman, A. Hrincu, D. Petsche, A. Poon, and EW Gelfand. "Separation of lymphoid and myeloid blasts in the mixed blast crisis of chronic myelogenous leukemia: no evidence for Ig gene rearrangement in CALLA-positive blasts." Blood 66, no. 6 (December 1, 1985): 1404–8. http://dx.doi.org/10.1182/blood.v66.6.1404.1404.
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Abstract Recent studies suggest that lymphoid blast crisis cells of chronic myelogenous leukemia (CML) expressing the common acute lymphoblastic leukemia antigen (CALLA) are B precursor cells, based on the demonstration of immunoglobulin (Ig) gene rearrangement similar to common acute lymphocytic leukemia. There is little evidence to suggest whether the cells with similar lymphoid characteristics in the mixed blast crisis of CML are also committed to B cell lineage. A patient in “mixed” blast crisis of CML was studied. On the basis of morphology, cytochemistry, and immunological studies, the blasts were classified as having either lymphoid or myeloid characteristics. A proportion of the leukemic blasts expressed CALLA, whereas others expressed My7 antigen. In order to characterize both populations of cell further, CALLA+ blasts and My7+ (myeloid) blasts were isolated by fluorescence- activated cell sorting. The My7+ cells were highly proliferative in cell culture blast colony assays, retained the Ph1 chromosome, and were indistinguishable from acute myelogenous leukemia blasts. The CALLA+ cells were also Ph1-chromosome positive, but in contrast, were poorly proliferative in vitro. Of particular note was their retention of germline configuration of Ig genes, thus distinguishing them from blasts in the lymphoid crisis of CML. We conclude that the lymphoid component in mixed blast crisis may represent a stage of differentiation prior to commitment to B lineage.
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Ha, K., MH Freedman, A. Hrincu, D. Petsche, A. Poon, and EW Gelfand. "Separation of lymphoid and myeloid blasts in the mixed blast crisis of chronic myelogenous leukemia: no evidence for Ig gene rearrangement in CALLA-positive blasts." Blood 66, no. 6 (December 1, 1985): 1404–8. http://dx.doi.org/10.1182/blood.v66.6.1404.bloodjournal6661404.
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Recent studies suggest that lymphoid blast crisis cells of chronic myelogenous leukemia (CML) expressing the common acute lymphoblastic leukemia antigen (CALLA) are B precursor cells, based on the demonstration of immunoglobulin (Ig) gene rearrangement similar to common acute lymphocytic leukemia. There is little evidence to suggest whether the cells with similar lymphoid characteristics in the mixed blast crisis of CML are also committed to B cell lineage. A patient in “mixed” blast crisis of CML was studied. On the basis of morphology, cytochemistry, and immunological studies, the blasts were classified as having either lymphoid or myeloid characteristics. A proportion of the leukemic blasts expressed CALLA, whereas others expressed My7 antigen. In order to characterize both populations of cell further, CALLA+ blasts and My7+ (myeloid) blasts were isolated by fluorescence- activated cell sorting. The My7+ cells were highly proliferative in cell culture blast colony assays, retained the Ph1 chromosome, and were indistinguishable from acute myelogenous leukemia blasts. The CALLA+ cells were also Ph1-chromosome positive, but in contrast, were poorly proliferative in vitro. Of particular note was their retention of germline configuration of Ig genes, thus distinguishing them from blasts in the lymphoid crisis of CML. We conclude that the lymphoid component in mixed blast crisis may represent a stage of differentiation prior to commitment to B lineage.
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Sainoki, Atsushi, and Hani S. Mitri. "Numerical simulation of rock mass vibrations induced by nearby production blast." Canadian Geotechnical Journal 51, no. 11 (November 2014): 1253–62. http://dx.doi.org/10.1139/cgj-2013-0480.
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It is of importance to understand the effect of production blasts on the surrounding rock formations in underground mines. This study presents a numerical procedure to simulate stress waves resulting from nearby production blasts. First, the damping coefficient and peak borehole pressure are calibrated using a dynamic numerical model of a single blast hole. The resulting time-varying particle velocities in the surrounding rock mass at the specified points are calculated. These are then used as input parameters in a three-dimensional mine-wide model, considering a positional relationship between the blast hole and the specified points on the wall rock. The mine-wide model encompasses a fault running parallel to a steeply dipping, tabular ore deposit. Dynamic analysis simulating the effect of production blasts is conducted after the extraction of mining blocks with static analysis. In this study, variations of stresses along the fault due to blast-induced stress waves are examined. Results demonstrate that the developed methodology can reasonably simulate stress changes induced by stress waves on the fault. The methodology considers blast sequences and time-varying blast loads that vary according to the positional relationship between the blast holes and the specified points on the wall rock.
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Singh, Rakesh Kumar, Chhangte Sawmliana, and Panchanan Hembram. "Damage threat to sensitive structures of a thermal power plant from hard rock blasting operations in track hopper area: A case study." International Journal of Protective Structures 11, no. 1 (April 17, 2019): 3–22. http://dx.doi.org/10.1177/2041419619843633.
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The article deals with the development of controlled blast design patterns and methodologies for excavation of hard rock for foundation work of a track hopper in a running thermal power plant in close proximity of many sensitive structures. Test blasts were conducted with different blast geometries and charge loading patterns at selected locations. The results of the test blasts were analysed to evolve safe controlled blast design patterns and methodologies for completing the total excavation work. The threshold value of vibration for the safety of various structures/foundations present has been taken as 25 mm/s based on the dominant frequency content as well as guidelines from the regulatory authority. The dominant frequency content of the ground vibration waves was in the range of 10–40 Hz. Controlled blast design parameters, namely maximum explosive per delay, size of the blasts and blast geometry were formulated based on established ground vibration predictor equation and nearness of structures concerned from the blasting point. Two blasting zones were classified for safe excavation work. For the first zone within 20 m from the structures, controlled blasting using 32-mm diameter blast holes with small blast geometry was suggested, whereas for the second zone beyond 20 m, controlled blasting with 100-mm diameter holes with lesser hole depth and limited number of holes was suggested. The whole excavation work in the track hopper area was completed safely without causing any damage to the nearby sensitive structures of the running thermal power plant.
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Race, Nicholas, Jesyin Lai, Riyi Shi, and Edward L. Bartlett. "Differences in postinjury auditory system pathophysiology after mild blast and nonblast acute acoustic trauma." Journal of Neurophysiology 118, no. 2 (August 1, 2017): 782–99. http://dx.doi.org/10.1152/jn.00710.2016.
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Blasts exposures often produce hearing difficulties. Although cochlear damage typically occurs, the downstream effects on central auditory processing are less clear. Moreover, outcomes were compared between individuals exposed to the blast pressure wave vs. those who experienced the blast noise without the pressure wave. It was found that a single blast exposure produced changes at all stages of the ascending auditory path at least 4 wk postblast, whereas blast noise alone produced largely transient changes.
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Kent, W. J. "BLAT---The BLAST-Like Alignment Tool." Genome Research 12, no. 4 (March 20, 2002): 656–64. http://dx.doi.org/10.1101/gr.229202.
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Kent, W. J. "BLAT---The BLAST-Like Alignment Tool." Genome Research 12, no. 4 (March 20, 2002): 656–64. http://dx.doi.org/10.1101/gr.229202.
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Westfall, Danielle E., Ling Zhang, Sophie Song, and Stephen Lee. "Concurrent Megakaryocytic and Erythroid Chronic Myelogenous Leukemia Blast Crisis." Archives of Pathology & Laboratory Medicine 132, no. 6 (June 1, 2008): 1021–25. http://dx.doi.org/10.5858/2008-132-1021-cmaecm.
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Abstract Chronic myelogenous leukemia with blast crisis is seen in 15% to 20% of patients with chronic myelogenous leukemia. Chronic myelogenous leukemia with either erythroid or megakaryocytic blast crisis is not uncommon in the clinical setting. The incidence ranges from 0% to 33% in accordance with literature reports. The diagnosis of erythroid or megakaryocytic blast phase is often challenging because the percentage of blasts in the blood or bone marrow required for diagnosis has not been firmly established. Also, some myeloblasts can have aberrant expression of either erythroid or megakaryocytic markers by flow cytometry during clonal evolution. Early recognition of this entity is crucial because either megakaryocytic or erythroid blast crisis predicts an aggressive clinical course. To our knowledge, the coexistence of megakaryocytic and erythroid blasts has not been reported. We report a unique case of chronic myelogenous leukemia with this rare bilineage blast crisis in the background of dysplasia and marked myelofibrosis. Related literature is also reviewed.
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Radford, Peter, Hasu D. L. Patel, Nicholas Hamilton, Mark Collins, and Steven Dryden. "Tympanic Membrane Rupture in the Survivors of the July 7, 2005, London Bombings." Otolaryngology–Head and Neck Surgery 145, no. 5 (June 9, 2011): 806–12. http://dx.doi.org/10.1177/0194599811411143.
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Objective. The goal of this study was to analyze the prevalence of tympanic membrane rupture in the survivors of the London bombings of July 2005 and to assess whether tympanic membrane rupture provides a useful biomarker for underlying primary blast injuries. Study Design. Cross-sectional study. Subjects and Methods. Survivors of the 4 blasts of London bombings on July 7, 2005. Data were gathered from medical records and the London’s Metropolitan Police evidence documenting the injuries sustained by 143 survivors of the blasts. All patients with tympanic membrane rupture or primary blast injury were indentified. Analysis was made of distance against prevalence of tympanic membrane rupture. Correlation between tympanic membrane rupture and other forms of primary blast injury was then assessed. Results. Results from the 143 survivors showed a 48% prevalence of tympanic membrane rupture across all 4 sites. Fifty-one patients had isolated tympanic membrane rupture with no other primary blast injuries. Eleven patients had tympanic membrane rupture and other primary blast injuries, but only one of these was an initially concealed injury (blast lung). Conclusions. Tympanic membrane rupture in survivors of the London bombings on July 7, 2005, had a high prevalence affecting half of patients across a range of distances from the blasts. Tympanic membrane did not act as an effective biomarker of underlying blast lung. In a mass casualty event, patients with isolated tympanic membrane rupture with normal observations and chest radiography can be monitored for a short period and safely discharged with arrangement for ear, nose, and throat follow-up.
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Rauch, Philipp J., Jana M. Ellegast, Corinne C. Widmer, Kristin Fritsch, Jeroen S. Goede, Peter J. M. Valk, Bob Löwenberg, Hitoshi Takizawa, and Markus G. Manz. "MPL expression on AML blasts predicts peripheral blood neutropenia and thrombocytopenia." Blood 128, no. 18 (November 3, 2016): 2253–57. http://dx.doi.org/10.1182/blood-2016-04-711986.
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Key Points AML-associated peripheral blood cytopenia is independent of bone marrow blast content, but strongly predicted by MPL expression on blast cells. MPLhi blasts scavenge TPO from serum, causing insufficient cytokine levels.
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Hadjioannou, Michalis, Aldo E. McKay, and Phillip C. Benshoof. "Full-Scale Blast Tests on a Conventionally Designed Three-Story Steel Braced Frame with Composite Floor Slabs." Vibration 4, no. 4 (November 24, 2021): 865–92. http://dx.doi.org/10.3390/vibration4040049.
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This paper summarizes the findings of two full-scale blasts tests on a steel braced frame structure with composite floor slabs, which are representative of a typical office building. The aim of this research study was to experimentally characterize the behavior of conventionally designed steel braced frames to blast loads when enclosed with conventional and blast-resistant façade. The two tests involved a three-story, steel braced frame with concentrical steel braces, which are designed to resist typical gravity and wind loads without design provisions for blast or earthquake loads. During the first blast test, the structure was enclosed with a typical, non-blast-resistant, curtainwall façade, and the steel frame sustained minimal damage. For the second blast test, the structure was enclosed with a blast-resistant façade, which resulted in higher damage levels with some brace connections rupturing, but the building did not collapse. Observations from the test program indicate the appreciable reserved capacity of steel brace frame structures to resist blast loads.
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Andika, I. Made Prasetia Candra, I. Made Anom Sutrisna Wijaya, and Ida Bagus Putu Gunadnya. "Pendugaan Intensitas Serangan Penyakit Blas pada Tanaman Padi Melalui Pendekatan Citra NDVI (Normalized Difference Vegetation Index)." Jurnal BETA (Biosistem dan Teknik Pertanian) 7, no. 2 (April 5, 2019): 287. http://dx.doi.org/10.24843/jbeta.2019.v07.i02.p09.
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Penyakit blas merupakan salah satu penyakit yang berbahaya bagi tanaman padi. Penyakit ini bisa menyerang di setiap fase pertumbuhan. Perhitungan intensitas serangan penyakit blas saat ini masih dilakukan secara manual. Diperlukan pengembangan teknologi dalam pendugaan intensitas serangan penyakit blas melalui citra NDVI. Penelitian ini bertujuan untuk (1) untuk mendapatkan ketinggian foto udara NDVI terbaik, (2) untuk mendapatkan umur tanaman padi dengan intensitas serangan penyakit blas tertinggi, (3) untuk mendapatkan hubungan antara intensitas serangan penyakit blas dengan nilai NDVI tanaman padi. Penelitian ini menggunakan Drone DJI Phantom 4 dengan lensa NDVI. Pengolahan data menggunakan Web Drone Deploy dan software Arc Gis 10.3. Berdasarkan dari hasil analisis, detail terbaik dari pembesaran 200% mendapatkan akuisisi ketinggian dari citra NDVI adalah 20 meter dengan ukuran piksel 1,4732 cm/pixel. Pertumbuhan intensitas serangan penyakit blas tertinggi terjadi pada umur 98 hari setelah tanam. Hubungan antara intensitas serangan penyakit blas dengan nilai NDVI memiliki koefisien determinasi sebesar 0,986. Persamaan regresi didapatkan dalam penelitian ini adalah y = -23345x3 + 21191x2- 6416,8x + 665,07 dengan akurasi sebesar sebesar 91,74%.
Blast is one of disease that is dangerous for rice plants. This disease can attack in every phase of growth. Calculation of the intensity of blast disease attacks is still done manually. Technology development is needed in estimating the intensity of blast disease attacks through NDVI imagery. This study purpose (1) to get the best NDVI aerial photo altitude, (2) to get the age of rice plants with the highest attack intensity of blast disease, (3) to get a relationship between the intensity of blast disease and the NDVI value of rice plants. This study use Drone DJI Phantom 4 with lens NDVI. Processing data using Web Drone Deploying and Arc Gis 10.3 software. Based on the analysis results, the best detail of 200% zooming results obtained altitude of the NDVI image acquisition that is 20 m with pixel density of 1,4732 cm/pixel. The highest intensity of blast disease attacks occurs at the age of 98 days after planting. The relationship between the intensity of blast disease and NDVI value has a determination coefficient of 0.986. The regression equation obtained in this study is y = -23345x3 + 21191x2- 6416,8x + 665,07 with an estimated accuracy of 91,74%.
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Zhuang, Yu, Aiguo Xing, Perry Bartelt, Muhammad Bilal, and Zhaowei Ding. "Dynamic response and breakage of trees subject to a landslide-induced air blast." Natural Hazards and Earth System Sciences 23, no. 4 (April 4, 2023): 1257–66. http://dx.doi.org/10.5194/nhess-23-1257-2023.
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Abstract. Landslides have been known to generate powerful air blasts capable of causing destruction and casualties far beyond the runout of sliding mass. The extent of tree damage provides valuable information on air blast intensity and impact region. However, little attention has been paid to the air blast–tree interaction. In this study, we proposed a framework to assess the tree destruction caused by powerful air blasts, including the eigenfrequency prediction method, tree motion equations and the breakage conditions. The tree is modeled as a flexible beam with variable cross-sections, and the anchorage stiffness is introduced to describe the tilt of the tree base. Large tree deflection is regarded when calculating the air blast loading, and two failure modes (bending and overturning) and the associated failure criteria are defined. Modeling results indicate that although the anchorage properties are of importance to the tree eigenfrequency, tree eigenfrequency is always close to the air blast frequency, causing a dynamic magnification effect for the tree deformation. This magnification effect is significant in cases with a low air blast velocity, while the large tree deflection caused by strong air blast loading would weaken this effect. Furthermore, failure modes of a specific forest subject to a powerful air blast depend heavily on the trunk bending strength and anchorage characteristics. The large variation in biometric and mechanical properties of trees necessitates the establishment of a regional database of tree parameters. Our work and the proposed method are expected to provide a better understanding of air blast power and to be of great use for air blast risk assessment in mountainous regions worldwide.
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NG, E. Y. K., and L. L. TAY. "STUDY OF BLAST DNA MATCHING TOOLKITS." Journal of Mechanics in Medicine and Biology 04, no. 03 (September 2004): 341–59. http://dx.doi.org/10.1142/s0219519404001090.
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The beginning of bioinformatics saw the development of algorithms that enabled the storage of nucleic acid and protein sequences in the form of annotated databases in a manner that would allow researchers to exchange information about gene and protein sequences easily and quickly. Databases are growing extremely fast, hence it is essential to use the current databases, which are easily available on the Web. This tutorial deals with the concept of DNA matching by using BLAST programs such as BLASTN and MEGABLAST to perform similarity sequence search and to evaluate their relative effectiveness. Interpretation of the BLAST results is done. Comparisons between the two algorithms are included based on varying parameters such as word sizes, query sequences length and gap X drop-off values, etc. It is found that as the word size increases, the computation time for both BLASTN and MEGABLAST algorithms decreases. BLASTN is more sensitive than MEGABLAST since it uses a shorter default word size of 11 as compared to MEGABLAST, which uses a default word size of 28. The search strategy offers a tradeoff between speed and sensitivity. As for BLAST 2 Sequences, MEGABLAST could perform better than BLASTN only for large word sizes greater than or equal to 16 and for longer sequences.
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Hunfalvay, Melissa, Nicholas P. Murray, William T. Creel, and Frederick R. Carrick. "Long-Term Effects of Low-Level Blast Exposure and High-Caliber Weapons Use in Military Special Operators." Brain Sciences 12, no. 5 (May 23, 2022): 679. http://dx.doi.org/10.3390/brainsci12050679.
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Chronic low-level blast exposure has been linked with neurological alterations and traumatic brain injury (TBI) biomarkers. Impaired smooth-pursuit eye movements (SPEM) are often associated with TBI. The purpose of this study was to determine whether long-term operators of low-level blast exposure or high-caliber weapons use displayed oculomotor behaviors that differed from controls. Twenty-six members of an elite military unit performed a computerized oculomotor testing task using an eye tracker and completed a concussion assessment questionnaire. The participants were split into a blast exposure group and control group. The blast exposure group had a history of exposure to low-level blasts or high-caliber weapon use. The results revealed significant differences in SPEM, saccades, and fixations between the blast exposure group and control group. The blast exposure group’s eye movements were slower, stopped at more frequent points when following a target, traveled further from the target in terms of both speed and direction, and showed higher rates of variation and inefficiency. Poor oculomotor behavior correlated with a higher symptom severity on the concussion assessment questionnaire. Military special operators exposed to long-term low-level blasts or high-caliber weapons usage displayed an impaired oculomotor behavior in comparison to controls. These findings further our understanding of the impact of long-term low-level blast exposure on the oculomotor behavior of military special operators and may inform practical implications for military training.
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Jiang, Shangyuan, Ariana N. Gannon, Kyle D. Smith, Marcus Brown, Junfeng Liang, and Rong Z. Gan. "Prevention of Blast-induced Auditory Injury Using 3D Printed Helmet and Hearing Protection Device – A Preliminary Study on Biomechanical Modeling and Animal." Military Medicine 186, Supplement_1 (January 1, 2021): 537–45. http://dx.doi.org/10.1093/milmed/usaa317.
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ABSTRACT Introduction Repeated blast exposures result in structural damage to the peripheral auditory system (PAS) and the central auditory system (CAS). However, it is difficult to differentiate injuries between two distinct pathways: the mechanical damage in the PAS caused by blast pressure waves transmitted through the ear and the damage in the CAS caused by blast wave impacts on the head or traumatic brain injury. This article reports a preliminary study using a 3D printed chinchilla “helmet” as a head protection device associated with the hearing protection devices (e.g., earplugs) to isolate the CAS damage from the PAS injuries under repeated blast exposures. Materials and Methods A finite element (FE) model of the chinchilla helmet was created based on micro-computed tomography images of a chinchilla skull and inputted into ANSYS for FE analysis on the helmet’s protection against blast over pressure. The helmet was then 3D printed and used for animal experiments. Chinchillas were divided into four cases (ears open, with earplug only, with both earplug and helmet, and with helmet only) and exposed to three blasts at blast over pressure of 15 to 20 psi. Hearing function tests (e.g., auditory brainstem response) were performed before and after blast on Day 1 and Days 4 and 7 after blasts. Results The FE model simulation showed a significant reduction in intracranial stress with the helmet, and the animal results indicated that both earplug and helmet reduced the severity of blast-induced auditory injuries by approximately 20 dB but with different mechanisms. Conclusions The biomechanical modeling and animal experiments demonstrated that this four-case study in chinchillas with helmet and hearing protection devices provides a novel methodology to investigate the blast-induced damage in the PAS and CAS.
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Agus, D. B., C. D. Surh, and J. Sprent. "Reentry of T cells to the adult thymus is restricted to activated T cells." Journal of Experimental Medicine 173, no. 5 (May 1, 1991): 1039–46. http://dx.doi.org/10.1084/jem.173.5.1039.
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To seek information on the capacity of mature T cells to migrate to the thymus, mice were injected with Thy-1-marked populations enriched for resting T cells or T blast cells; localization of the donor cells in the host thymus was assessed by staining cryostat sections of thymus and by FACS analysis of cell suspensions. With injection of purified resting T cells, thymic homing was extremely limited, even with injection of large doses of cells. By contrast, in vivo generated T blast cells migrated to the thymus in substantial numbers. Thymic homing by T blasts was greater than 50-fold more efficient than with resting T cells. Blast cells localized largely in the medulla and remained in the thymus for at least 1 mo post-transfer. Interestingly, localization of T blasts in the thymus was 10-fold higher in irradiated hosts than normal hosts. Thymic homing was especially prominent in mice injected with T blasts incubated in vitro with the DNA precursor, 125I-5-iodo-2'deoxyuridine (125IDUR); with transfer of 125IDUR-labeled blasts to irradiated hosts, up to 5% of the injected counts localized in the host thymus. These data suggest that thymic homing by T blasts might be largely restricted to cells in S phase. The physiological significance of blast cell entry to the thymus is unclear. The possibility that these cells participate in intrathymic tolerance induction is discussed.
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Sutherland, HJ, A. Blair, and RW Zapf. "Characterization of a hierarchy in human acute myeloid leukemia progenitor cells." Blood 87, no. 11 (June 1, 1996): 4754–61. http://dx.doi.org/10.1182/blood.v87.11.4754.bloodjournal87114754.
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Despite the usual uniform and primitive appearance of cells derived from the leukemic clone in most patients with acute myeloid leukemia (AML), there is considerable heterogeneity among leukemic blasts, particularly with respect to their capacity to proliferate and/or self renew. We have assessed whether these differences in proliferative potential are correlated with the phenotypic changes that characterize normal hematopoiesis, which might suggest an analogous hierarchy of AML progenitors. We have used the ability of primitive AML cells to persist or produce blast colony forming cells (CFU-blast) detected after 2 to 8 weeks in the presence of growth factors in suspension cultures (SC) termed SC-initiating cells (IC), or with stroma in long-term cultures (LTC-IC) as a quantitative assay for a cell that may have primitive characteristics. This SC assay is linear, cell concentration independent, and the frequency of SC-IC by limiting dilution analysis is lower than primary CFU-blast. The average output of CFU-blast after 2 to 8 weeks by individual SC-IC varied between 2 and more than 100 in individual patients. Leukemic blasts were sorted based on their expression of antigens previously found useful to characterize normal progenitor differentiation, and analyzed for the percentage of CFU- blast SC-IC, and leukemic LTC-IC within each fraction. All of these progenitor types were heterogeneous in their expression of CD45RA and CD33, but expressed uniformly low levels of CD15 and differed from normal primitive progenitors in their high expression of HLA-DR. CFU- blast had a significantly higher expression of CD71 and CD38 as compared with SC-IC or leukemic LTC-IC. In patients with CD34+ blasts, the majority of their SC-IC at 4 weeks were CD34+/CD38-; however, patients with CD34- blasts had at least some CD34- progenitors. These results show that while heterogeneity exists between patients, it is possible to physically separate subpopulations of AML cells with different proliferative potentials. It also provides some support for the concept that quantitation of leukemic cells capable of producing CFU-blast for 4 weeks or more in vitro measures a less frequent leukemic progenitor with higher proliferative potential that may be the only relevant cell for maintaining the leukemic clone in vivo.
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Yogarajah, Meera, Phuong L. Nguyen, Rong He, Hassan Alkhateeb, Mithun Vinod Shah, Mrinal Mahesh Patnaik, Naseema Gangat, et al. "Discrepancy of blast percentage between the bone marrow aspirate and biopsy and its impact on survival outcomes in patients with myelodysplastic syndromes excess blast (MDS-EB)." Journal of Clinical Oncology 37, no. 15_suppl (May 20, 2019): 7053. http://dx.doi.org/10.1200/jco.2019.37.15_suppl.7053.
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7053 Background: The revised International Prognostic Scoring System (IPSS-R) aids in prognosticating MDS. The percentage (%) of blasts in the bone marrow is one of the major determinants of the scoring system. The aspirate blast % is utilized as the standard of care, but there could be discrepancies in the blast % reported by the aspirate and the biopsy. We aim to study the possible use of bone marrow biopsy blasts in MDS-EB in calculating IPSS-R. Methods: The MDS database was reviewed for cases of MDS-EB after due IRB approval. We calculated IPSS-R scores based on the aspirate blast % (IPSS-RAsp) and biopsy blast % (IPSS-RBx). The biopsy blast % was reported morphologically or by the CD34 stain. Whenever a range was reported the highest value was utilized as the blast %. Suboptimal aspirates were excluded from the study. The overall survival (OS) was determined by IPSS-RAsp, IPSS-RBx and IPSS-R highest blast (IPSS-RHi). OS estimates were calculated by Kaplan-Meier curves and log-rank testing using JMP v.13. Uno’s concordance statistic was used to compare all 3 risk scoring systems. Results: Of 1322 patients, 431 (33%) cases were identified with MDS-EB; out of which 173 cases had both blasts reported in the biopsy and the aspirate. Out of 173 cases, 35 (20%) had MDS-EB1, and 61 (35%) had MDS EB-2 based on both biopsy and aspirate (concordant cases). Seventy seven (45%) patients changed from EB-1 to EB2 or vice versa based on the biopsy blast (44/77 (57%) cases were upstaged). The OS outcomes based on the IPSS-RBx biopsy showed a clear and meaningful separation with median OS decreasing with increased risk but IPSS-RAsp and IPSS-RHi did not (Table). We compared the 3 models for observed OS differences using the Uno model and there was no statistically significant difference. Conclusions: IPSS-RBx (but not IPSS-RAsp and IPSS-RHi) identified prognostic groups for OS with median OS decreasing with increased risk. The small sample size may have led to an insignificant effect on model power by Uno model. This finding needs to be validated by other centers. [Table: see text]
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Kim, Jung H., James A. Goodrich, Robert Situ, Amedeo Rapuano, Hoby Hetherington, Fu Du, Steve Parks, et al. "Periventricular White Matter Alterations From Explosive Blast in a Large Animal Model: Mild Traumatic Brain Injury or “Subconcussive” Injury?" Journal of Neuropathology & Experimental Neurology 79, no. 6 (April 7, 2020): 605–17. http://dx.doi.org/10.1093/jnen/nlaa026.
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Abstract The neuropathology of mild traumatic brain injury in humans resulting from exposure to explosive blast is poorly understood as this condition is rarely fatal. A large animal model may better reflect the injury patterns in humans. We investigated the effect of explosive blasts on the constrained head minimizing the effects of whole head motion. Anesthetized Yucatan minipigs, with body and head restrained, were placed in a 3-walled test structure and exposed to 1, 2, or 3 explosive blast shock waves of the same intensity. Axonal injury was studied 3 weeks to 8 months postblast using β-amyloid precursor protein immunohistochemistry. Injury was confined to the periventricular white matter as early as 3–5 weeks after exposure to a single blast. The pattern was also present at 8 months postblast. Animals exposed to 2 and 3 blasts had more axonal injury than those exposed to a single blast. Although such increases in axonal injury may relate to the longer postblast survival time, it may also be due to the increased number of blast exposures. It is possible that the injury observed is due to a condition akin to mild traumatic brain injury or subconcussive injury in humans, and that periventricular injury may have neuropsychiatric implications.
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Elliott, Michelle A., Mark R. Litzow, Louis Letendre, Robert C. Wolf, Curtis A. Hanson, Ayalew Tefferi, and Martin S. Tallman. "Early Peripheral Blood Blast Clearance during Induction Chemotherapy for Acute Myeloid Leukemia (AML) Predicts Superior Relapse-Free Survival." Blood 110, no. 11 (November 16, 2007): 1848. http://dx.doi.org/10.1182/blood.v110.11.1848.1848.
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Abstract Background: In childhood acute lymphoblastic leukemia (ALL), a rapid decline of circulating blasts in response to induction chemotherapy or prednisone is one of the most important prognostic factors for achieving remission and for relapse-free survival (RFS). However, in AML parameters of chemosensitivity have been restricted to the assessment of residual bone marrow (BM) blasts during aplasia. We hypothesized that time to peripheral blood (PB) blast clearance would have prognostic relevance with respect to RFS in AML. Methods: From 1994 to 2006 outcomes of 86 adults (47 males; median age 52 y) with previously untreated AML (non- acute promyelocytic leukemia) achieving CR and receiving high-dose cytarabine (HDAC)-based consolidation have been included in this retrospective analysis. The median and range of white blood cell count (WBC), platelet count and PB blast percent were 5.3 × 109/L (0.9–267), 59 ×109/L (5–361) 22.5% (0–97), respectively. A 100 cell manual slide differential count was done in all with PB blasts unless the WBC was less than 0.5 ×109/L. Karyotype was classified as favorable, intermediate and unfavorable in 10, 63 and 12 cases, respectively and failed in one. Induction consisted of idarubicin at 12 mg/m2/ d (n=70) or daunorubicin at 45 mg/m2/d (n=16) on days 1–3 with infusional cytarabine at 100 mg/m2/ d on days 1–7 and repeated in those with persistent BM blasts on day 14 (n=17). The first consolidation was the same as that used to achieve CR. Thereafter, 3 courses of HDAC at 3g/m2 (1.5 g/m2 if ≥ 60 y) q12 h on days 1, 3 and 5 were planned. Results: At the time of analyses, 43% had died, primarily of relapse (97%). Median overall survival (OS) and RFS were 30.2 m (5.5–133) and 14 m (2–131.5), respectively. The median time to PB blast clearance was five days (range: 2–10). For this analysis, only those with PB blasts at initiation of induction (n=73) were included. We defined the day of PB blast clearance as the first day after commencing induction that PB blasts were absent. Separation according to blast clearance on or before day 5 resulted in the most balanced distribution and strongest significant difference between each subgroup of 45 and 28 patients, with significantly different rates of relapse of 33% and 79%, respectively (p<0.0001). We also defined three “blast risk groups” as good, intermediate and poor, according to PB blast clearance on or before day 3, on days 4 or 5, or on day 6 or beyond, respectively. This provided 3 well-balanced groups (good, intermediate and poor) of 16, 30 and 27 patients with significantly different and escalating relapse rates of 12.5%, 47% and 78%, respectively (Fig 1). Univariate analysis of several variables identified PB blast clearance day (p<0.0001), number of inductions to CR (p =0.0043) and cytogenetic risk group (p=0.028) as being significantly associated with RFS. On multivariable analysis, only the first sustained its significance. The results were similar for OS. Conclusion: In adults who achieve CR after induction chemotherapy for non-APL AML, early PB blast clearance predicts superior RFS and OS. Figure Figure
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Fernández, Pablo R., Rafael Rodríguez, and Marc Bascompta. "Holistic Approach to Define the Blast Design in Quarrying." Minerals 12, no. 2 (January 31, 2022): 191. http://dx.doi.org/10.3390/min12020191.
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A new approach to determine the most efficient blast design was proposed, taking the peak particle velocity (PPV) as the main constraint, while including costs, production, granulometry, air blast and carbon footprint as relevant characteristics. The research was based on a case study that registered and analyzed 548 blasts over more than 10 years, while the vibration of the blast was recorded in 32% of them. A general attenuation law and several local laws were determined based on the geological conditions of the quarry. The blast design improvement allowed one to reduce the specific consumption by almost 30%, 26% of the blast cost in terms of explosive consumption and 18% of the CO2 emissions. This allowed for the achievement of a more environmentally friendly extraction system while complying with the legal standards, the quality requirements and the production and productivity conditions established.
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Gasic, Vladimir, Branka Zukic, Biljana Stankovic, Dragana Janic, Lidija Dokmanovic, Jelena Lazic, Nada Krstovski, et al. "Pharmacogenomic markers of glucocorticoid response in the initial phase of remission induction therapy in childhood acute lymphoblastic leukemia." Radiology and Oncology 52, no. 3 (September 11, 2018): 296–306. http://dx.doi.org/10.2478/raon-2018-0034.
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AbstractBackgroundResponse to glucocorticoid (GC) monotherapy in the initial phase of remission induction treatment in childhood acute lymphoblastic leukemia (ALL) represents important biomarker of prognosis and outcome. We aimed to study variants in several pharmacogenes (NR3C1,GSTsandABCB1) that could contribute to improvement of GC response through personalization of GC therapy.MethodsRetrospective study enrolling 122 ALL patients was carried out to analyze variants ofNR3C1(rs33389, rs33388 and rs6198),GSTT1(null genotype),GSTM1(null genotype),GSTP1(rs1695 and rs1138272) andABCB1(rs1128503, rs2032582 and rs1045642) genes using PCR-based methodology. The marker of GC response was blast count per microliter of peripheral blood on treatment day 8. We carried out analysis in which cut-off value for GC response was 1000 (according to Berlin-Frankfurt-Munster [BFM] protocol), as well as 100 or 0 blasts per microliter.ResultsCarriers of rareNR3C1rs6198 GG genotype were more likely to have blast count over 1000, than the non-carriers (p = 0.030).NR3C1CAA (rs33389-rs33388-rs6198) haplotype was associated with blast number below 1000 (p = 0.030).GSTP1GC haplotype carriers were more likely to have blast number below 1000 (p = 0.036), below 100 (p = 0.028) and to be blast negative (p = 0.054), whileGSTP1GT haplotype and rs1138272 T allele carriers were more likely to be blasts positive (p = 0.034 and p = 0.024, respectively).ABCB1CGT (rs1128503-rs2032582-rs1045642) haplotype carriers were more likely to be blast positive (p = 0.018).ConclusionsOur results have shown thatNR3C1rs6198 variant andGSTP1rs1695-rs1138272 haplotype are the most promising pharmacogenomic markers of GC response in ALL patients.
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Devoe, Craig E., Delong Bao, Christina Trotta, Chung-Shien Lee, and Joanna Stein Fishbein. "Evaluation of prognostic factors after induction therapy in AML." Journal of Clinical Oncology 37, no. 15_suppl (May 20, 2019): e18528-e18528. http://dx.doi.org/10.1200/jco.2019.37.15_suppl.e18528.
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e18528 Background: Acute myeloid leukemia (AML) is a hematologic myeloid malignancy that carries a poor prognosis. Standard of care is induction chemotherapy with daunorubicin and cytarabine also known as 7 + 3. Bone marrow aspirates are assessed on day 14 (D14) and day 28 (D28). Achievement of CR (complete remission) is predicative of overall survival (OS ).Morphological review of BM (bone marrow) on D14 remains the standard evaluation for any indication for CR. Methods: Our primary objective was to determine if blast % on the D14 bone marrow aspirate is a prognostic indicator for remission in acute leukemia patients who underwent induction chemotherapy. Institutional review board was approved. A retrospective chart review was conducted at North Shore University Hospital. Adult patients with AML who received standard induction chemotherapy from 2010 to 2015 were included. 150 patients were reviewed and their D14 blast %, initial marrow blast %, gender, age, cytogenetics and initial WBC were assessed. The patients’ D14 blast % biopsies were divided into blast percentiles: < 1%, 1-10%, 10-30%, 30-60% and > 60%. CR was defined as patients having < 5% blasts on their D28 bone marrow or day 63 and corresponding neutrophil count > 1,000 and platelet count > 100,000. Results: Fisher’s exact test was used to compare the proportion of patients who reached CR among the D14 blast % categories. The Wilcoxon test was utilized to compare CR on continuous variables. Results were considered statistically significant if p < 0.05. D14 hemoglobin and D14 platelets were not significantly associated with CR status (p = 0.67, p = 0.33, and p = 0.13, respectively). D14 WBC was significantly associated with CR (p = 0.02), but when both D14 WBC and initial blasts % were included in a multiple logistic model, WBC was no longer a significant predictor of outcome. Only initial blast percent at time of diagnosis was deemed predictive of CR response at day D63 (p = 0.0142). Conclusions: Initial blast > 60% was found to be significantly associated with the greatest CR rate. This study provides evidence of initial blast % being a prognostic factor for CR.
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Schomer, Paul. "Attention to rattles and a non-equal-energy model are required for proper sonic boom assessment." Journal of the Acoustical Society of America 153, no. 3_supplement (March 1, 2023): A275. http://dx.doi.org/10.1121/10.0018829.
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This paper is concerned with the assessment of community response to sonic booms or blasts. It summarizes and analyzes the totality of results from studies in the English language that used real booms or blasts, with subjects in real buildings. In acoustics, we are accustomed to noise sources operating in accordance with the equal-energy principle (a 1 dB increase in amplitude is equivalent to a 1 dB increase in duration). The results show that rattles are the most important attribute contributing to the annoyance engendered by sonic booms/blasts, and that the process is not equal-energy. Rather, the equivalent annoyance generated by a change of 1 dB in the C-weighted boom or blast amplitude is equal to about a 1.5 to 2 dB change in the boom or blast duration where the exchange rate is defined to be 1 over these changes in duration, 0.67 and 0.5, respectively. The exchange rates found in several sonic boom/blast noise studies are given, and as an example, the exchange rate for the historical Oklahoma City study is calculated. The conclusions from the Long-Term Sonic Boom Noise Environments study are examined in relation to the range of exchange rates found in other boom/blast studies.
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Malekian, Maral, Moe Momayez, Pat Bellett, Stefania Mancino, and Fernanda Carrea. "WALL CONTROL AND BLAST PATTERN ANALYSIS USING BLAST VISION® TECHNOLOGY." American Journal of Interdisciplinary Innovations and Research 05, no. 04 (April 17, 2023): 31–37. http://dx.doi.org/10.37547/tajiir/volume05issue04-03.
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Wall control and blasting are two major activities that significantly impact the productivity and safety of open-pit mining operations. Although many monitoring equipment are used for improving slope stability, there is still a need for understanding slope behaviour during a blast. This paper describes a new technology called Blast Vision®, developed by Ground Probe, for monitoring the interaction between blasting and slope stability with a temporal resolution in the range of milliseconds. This technology represents an innovative departure from standard blast vibration sensors by measuring ground luminance changes on the ground surface through drone-based computer vision. Blast Vision application for slope stability, misfires and fly rocks is investigated in this publication.
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Bao, Delong, Chung-Shien Lee, Christina E. Trotta, and Craig Devoe. "Evaluation of Prognostic Factors after Induction Chemotherapy for Acute Myeloid Leukemia." Blood 132, Supplement 1 (November 29, 2018): 5269. http://dx.doi.org/10.1182/blood-2018-99-119830.
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Abstract Purpose: Recent studies have shown that dose escalation of daunorubicin from 45mg/m2 to 90mg/m2 during acute myeloid leukemia (AML) induction showed response benefit and overall survival in patients <=65 years. Yet, the further management of patients with persistent disease based on the evaluation of D14 bone marrow has often been questioned due to lack of data. The primary purpose of this study is to evaluate the overall remission rates of patients with a positive D14 bone marrow compared with those who had a negative D14 results, and to determine if blast % on the D14 bone marrow aspirate is a prognostic indicator for remission and survival in acute leukemia patients who underwent induction chemotherapy. We aim to investigate the relevance of the D14 bone marrow result by dividing the result into blast percent categories and investigate if the there is a correlation between D14 blast % and CR. Patients/Methods: This was an IRB approved retrospective chart review conducted at North Shore University Hospital. Adult patients with AML who received standard induction 7 + 3 Daunorubicin and Cytarabine from 2010 to 2015 were included. 150 patients were reviewed and those that were eligible were evaluated for various factors (D14 blast %, initial marrow blast %, gender, age, cytogenetic risk profile, initial WBC, initial hemoglobin, initial platelets and initial LDH levels, along with those lab values at D14) for tests of association with CR. These patients' D14 blast % biopsies were divided into blast percent categories as followed: (Chemotherapeutic/<1%, 1-10%, 10-30%, 30-60% and > 60%). Complete remission (CR) was defined as patients having <5% blasts on their day 28 bone marrow or day 63 and corresponding neutrophil count >1,000 and platelet count >100 k. Fisher's exact test was used to compare the proportion of patients who reached CR among the D14 blast % categories, and on other categorical data. The Wilcoxon test was utilized to compare CR on continuous variables. Results were considered statistically significant if p < 0.05. Results: 115 patients were analyzed and we found no significant association between D14 blast % and CR status. However, initial blast % was found to be significantly associated with CR status (p=0.009), specifically, those with >60% initially had the greatest CR rate. D14 hemoglobin, D14 platelets or D14 LDH levels were not significantly associated with CR status (p=0.67, p=0.33, and p=0.13, respectively). Similarly, initial WBC, hemoglobin, platelets and LDH levels were each not significantly associated with CR (p=0.99, p=0.51, p=0.47 and p=0.36, respectively). Results did provide evidence to suggest that D14 WBC was significantly associated with CR (p=0.02), but when both D14 WBC and initial blasts % were included in a multiple logistic model, WBC was no longer a significant predictor of outcome. As such, only initial blast percent (at time of diagnosis or initial visit) was deemed predictive of CR response day at D63 (p=0.0142); patients with initial blast >60% had the greatest rate of CR compared with <20% blasts initially. Conclusion: The data suggests that there is no statistical difference between the blast % on the D14 bone marrow and the achievement of CR during induction chemotherapy for AML. Secondary outcomes showed that the initial blast % was associated with CR especially in patients with an initial blast % >60%. Also, D14 WBC might be associated with remission. Prospective studies are required to confirm these findings. Disclosures No relevant conflicts of interest to declare.
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Loken, Michael R., Sung-Chao Chu, Wayne K. Fritschle, Dian-Kun Li, and Denise A. Wells. "Correcting Flow Cytometric Blast Counts for Blood Contamination in Bone Marrow Aspirates." Blood 110, no. 11 (November 16, 2007): 4613. http://dx.doi.org/10.1182/blood.v110.11.4613.4613.
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Abstract An accurate blast count is pivotal in the diagnosis, classification and prognosis of patients with myelodysplasia. Blast counts in all previous classification schemes are based on morphologic assessment of marrow aspirates with a poor correlation to blast counts determined by flow cytometry. A significant problem in blast enumeration by flow cytometry is the variable hemodilution of the marrow during collection for flow cytometric analysis. Blast counts can vary depending on which aspirate tube is used for flow analysis, e.g., 2.4%, 1st 5ml tube; 0.62%, 2nd 5ml tube; 0.58% 3rd 5ml tube. Morphologists circumvent this problem by selecting a region for assessment close to a spicule with minimal blood dilution. Cell surface antigens can be used to distinguish mature cells found in blood as distinct from immature cells identified in marrow. CD16 intensity on neutrophils reaches a maximum at the band/segmented stage of development with a low coefficient of variation, thereby becoming a marker for mature myeloid forms. A simple method to distinguish immature from mature myeloid cells was developed to assess extent of blood contamination in marrow aspirates using a combination of CD16, CD13, and CD45. The average mature neutrophil content of a marrow was determined from phenotypically normal bone marrow biopsy specimens, assumed to have minimal blood contamination. The proportion of dimCD16 cells gated on the myeloid cells based on CD45 and right angle light scatter in 31 biopsy specimens was 82% (range 69–93, SD=6.2) (Figure 1). A value of 80% (rather than 82%) was used for the subsequent calculations to correct for the excess mature neutrophils found in an aspirate as compared to the biopsies (Corrected Blasts = [80 / % dim CD16 myeloid] x determined blast count). To test this hypothesis bone marrow aspirates were diluted with blood at different ratios to mimic blood marrow hemodilution. Blasts (defined as CD45 dim, low right angle light scatter, HLA-DR positive, CD11b negative) were determined for the various dilutions, then corrected based solely on the proportion of dim CD16 myeloid cells (Figure 2). A marrow from an MDS case was also diluted (1:5 v/v) with blood for comparison. The original marrow contained 80% dim CD16 myeloid cells with a blast count of 9.2%. After dilution, only 12% dim CD16 cells were detected with 1.1% blasts, however upon correction (6.67), the blast count was 7.3%, close to the original determination. This approach may provide for more standardization and consistency in the determination of blast counts in MDS marrow specimens using flow cytometric analysis. Figure 1, CD16 of marrow myeloid cells. Figure 1,. CD16 of marrow myeloid cells. Figure 2, Uncorrected/Corrected Blast Count Figure 2,. Uncorrected/Corrected Blast Count
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Murohashi, I., S. Tohda, T. Suzuki, K. Nagata, Y. Yamashita, and N. Nara. "Autocrine growth mechanisms of the progenitors of blast cells in acute myeloblastic leukemia." Blood 74, no. 1 (July 1, 1989): 35–41. http://dx.doi.org/10.1182/blood.v74.1.35.35.
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Abstract Autocrine growth mechanisms of leukemic blast progenitors in acute myeloblastic leukemia (AML) were investigated. Colony formation of leukemic blast progenitors was observed in 14 of 14 patients tested when purified blast cell fraction depleted of both T cells and monocytes was plated in methylcellulose without any colony-stimulating factor (CSF). However, there existed a minimal cell density required to initiate blast progenitor growth with marked patient-to-patient variation. To clarify the role of cell density on the spontaneous growth of blast progenitors, we tested whether leukemic cells produced and secreted some stimulatory humoral factor(s). Production of colony- stimulating activity (CSA) by blast cells was observed in 17 of 18 patients tested. Following further depletion of monocytes, the CSA levels decreased markedly in 14 patients, indicating that blast cells with monocytoid differentiation were responsible for CSA production. We also confirmed granulocyte colony-stimulating factor (G-CSF) and/or granulocyte macrophage-colony-stimulating factor (GM-CSF) production by leukemic blasts using specific immunologic assays. When leukemic cells were divided into nonadherent nonphagocytic cell fraction and adherent cell fraction, only nonadherent nonphagocytic cells showed clonogenecity and adherent blast cells lacked the colony-forming capacity. The results indicate that there are at least two blast cell subpopulations in AML: one is proliferating subpopulation with self- renewal capacity and the other is supporting subpopulation with functions such as CSF production. The quite intimate relationship between these two blast cell subpopulations in AML may play an important role on the growth of leukemic blast progenitors in vitro.
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Murohashi, I., S. Tohda, T. Suzuki, K. Nagata, Y. Yamashita, and N. Nara. "Autocrine growth mechanisms of the progenitors of blast cells in acute myeloblastic leukemia." Blood 74, no. 1 (July 1, 1989): 35–41. http://dx.doi.org/10.1182/blood.v74.1.35.bloodjournal74135.
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Autocrine growth mechanisms of leukemic blast progenitors in acute myeloblastic leukemia (AML) were investigated. Colony formation of leukemic blast progenitors was observed in 14 of 14 patients tested when purified blast cell fraction depleted of both T cells and monocytes was plated in methylcellulose without any colony-stimulating factor (CSF). However, there existed a minimal cell density required to initiate blast progenitor growth with marked patient-to-patient variation. To clarify the role of cell density on the spontaneous growth of blast progenitors, we tested whether leukemic cells produced and secreted some stimulatory humoral factor(s). Production of colony- stimulating activity (CSA) by blast cells was observed in 17 of 18 patients tested. Following further depletion of monocytes, the CSA levels decreased markedly in 14 patients, indicating that blast cells with monocytoid differentiation were responsible for CSA production. We also confirmed granulocyte colony-stimulating factor (G-CSF) and/or granulocyte macrophage-colony-stimulating factor (GM-CSF) production by leukemic blasts using specific immunologic assays. When leukemic cells were divided into nonadherent nonphagocytic cell fraction and adherent cell fraction, only nonadherent nonphagocytic cells showed clonogenecity and adherent blast cells lacked the colony-forming capacity. The results indicate that there are at least two blast cell subpopulations in AML: one is proliferating subpopulation with self- renewal capacity and the other is supporting subpopulation with functions such as CSF production. The quite intimate relationship between these two blast cell subpopulations in AML may play an important role on the growth of leukemic blast progenitors in vitro.
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Slingerland, JM, MD Minden, and S. Benchimol. "Mutation of the p53 gene in human acute myelogenous leukemia." Blood 77, no. 7 (April 1, 1991): 1500–1507. http://dx.doi.org/10.1182/blood.v77.7.1500.1500.
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Abstract Heterogeneity of p53 protein expression is seen in blast cells of patients with acute myelogenous leukemia (AML). p53 protein is detected in the blasts of certain AML patients but not in others. We have identified p53 protein variants with abnormal mobility on gel electrophoresis and/or prolonged half-life (t 1/2). We have sequenced the p53 coding sequence from primary blast cells of five AML patients and from the AML cell line (OCIM2). In OCIM2, a point mutation in codon 274 was identified that changes a valine residue to aspartic acid. A wild type p53 allele was not detected in these cells. Two point mutations (codon 135, cysteine to serine; codon 246, methionine to valine) were identified in cDNA from blasts of one AML patient. Both mutations were present in blast colonies grown from single blast progenitor cells, indicating that individual leukemia cells had sustained mutation of both p53 alleles. The cDNAs sequenced from blast samples of four other patients, including one with prolonged p53 protein t 1/2 and one with no detectable p53 protein, were fully wild type. Thus, the heterogeneity of p53 expression cannot be explained in all cases by genetic change in the p53 coding sequence. The prolonged t 1/2 of p53 protein seen in some AML blasts may therefore reflect changes not inherent to p53. A model is proposed in which mutational inactivation of p53, although not required for the evolution of neoplasia, would confer a selective advantage, favoring clonal outgrowth during disease progression.
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Slingerland, JM, MD Minden, and S. Benchimol. "Mutation of the p53 gene in human acute myelogenous leukemia." Blood 77, no. 7 (April 1, 1991): 1500–1507. http://dx.doi.org/10.1182/blood.v77.7.1500.bloodjournal7771500.
Full textAbstract:
Heterogeneity of p53 protein expression is seen in blast cells of patients with acute myelogenous leukemia (AML). p53 protein is detected in the blasts of certain AML patients but not in others. We have identified p53 protein variants with abnormal mobility on gel electrophoresis and/or prolonged half-life (t 1/2). We have sequenced the p53 coding sequence from primary blast cells of five AML patients and from the AML cell line (OCIM2). In OCIM2, a point mutation in codon 274 was identified that changes a valine residue to aspartic acid. A wild type p53 allele was not detected in these cells. Two point mutations (codon 135, cysteine to serine; codon 246, methionine to valine) were identified in cDNA from blasts of one AML patient. Both mutations were present in blast colonies grown from single blast progenitor cells, indicating that individual leukemia cells had sustained mutation of both p53 alleles. The cDNAs sequenced from blast samples of four other patients, including one with prolonged p53 protein t 1/2 and one with no detectable p53 protein, were fully wild type. Thus, the heterogeneity of p53 expression cannot be explained in all cases by genetic change in the p53 coding sequence. The prolonged t 1/2 of p53 protein seen in some AML blasts may therefore reflect changes not inherent to p53. A model is proposed in which mutational inactivation of p53, although not required for the evolution of neoplasia, would confer a selective advantage, favoring clonal outgrowth during disease progression.
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Shponka, V., S. Kroft, A. M. Harrington, K. Monahan, and E. Atallah. "Non-Acute Myeloid Neoplasms With CD34(-) Blasts: Immunophenotypic And Clinical Analysis Of 5 Cases." American Journal of Clinical Pathology 154, Supplement_1 (October 2020): S105—S106. http://dx.doi.org/10.1093/ajcp/aqaa161.231.
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Abstract Introduction/Objective Non-acute myeloid neoplasms (NAMNs) comprise clonal hematopoietic disorders, characterized by ≤20% myeloblasts in the peripheral blood (PB) and/or bone marrow (BM). The blasts of NAMN are almost exclusively CD34(+), contrasting with blasts in some acute myeloid leukemias (AML). We detail the clinicopathologic features of NAMN with CD34(-) blasts. Methods We searched 981 NAMN pathology reports for CD34(-) blasts by flow cytometry (FC). 8-color FC data was analyzed using cluster analysis to identify all relevant cell populations with a comprehensive antibody panel. CD34(-) blast populations were defined as reproducible clusters with similar light scatter properties across antibody tubes, after exclusion of other cell populations (e.g. basophils, neutrophils, mast cells). Blast aberrancies were defined as differences from established normal antigen expression patterns. PB and BM morphology was reviewed and chart reviews were performed. Results We identified 5 NAMN patients with CD34(-) blasts (2M, 3F; 57-84y/o) including 3 myelodysplastic syndrome (MDSs) with excess blasts, 1 therapy-related MDS, and 1 chronic myelomonocytic leukemia-1 (CMML). Blasts ranged from 0-1.5% in PB and 2.8-8% in BM. CD34(-) blasts accounted for 0.85-8.2% of BM events by FC, with all showing other aberrancies. The CMML patient had CD34(-) blasts with monocytic differentiation; all other CD34(-) blast populations showed expression of CD13, CD33, and CD117 and aberrant under-expression of CD38. Aberrancies were also present in the CD34(+) blasts in all patients (1-4/patient), accounting for 0.01-0.55% of events; 3/5 pts had under-expression of CD33, CD38, CD45, and/or HLA-DR. No cases had adverse cytogenetics. Patient outcomes included: indolent disease (1); died from disease 3 years post-diagnosis and chemotherapy (1); transformed to AML (2; 1-alive in complete remission; 1-died); and alive following allogeneic stem cell transplant (1). Conclusion Our data confirm that CD34(-) blasts are rarely observed in NAMN, and these patients have variable outcomes. Interestingly, all cases showed a concomitant aberrant CD34(+) blast population.
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Collier, Zachary J., Katherine J. Choi, Ian F. Hulsebos, Christopher H. Pham, Haig A. Yenikomshian, and Justin Gillenwater. "123 A Novel Way of Thinking About Blast Injury Classification." Journal of Burn Care & Research 42, Supplement_1 (April 1, 2021): S82—S83. http://dx.doi.org/10.1093/jbcr/irab032.127.
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Abstract Introduction Blast injuries present unique challenges to civilian and military healthcare providers because of the complex and often severe nature of injuries spanning numerous anatomical regions, tissue types, and organ systems. Due to these factors, we devised a novel wound-focused classification system for implementation during triage and management of blast injuries to optimize outcomes and applied this system to patients treated at an ABA-certified burn center over 5 years. Methods A retrospective analysis of patients treated by an ABA-certified burn center for blast-related injuries from September 1, 2014 to October 31, 2019 was performed. Demographics, mechanism and distribution of injuries, interventions, and outcomes were evaluated. Injuries were classified using a wound-focused classification comprised of four zones: 1) areas closest to blast epicenter that had total or near-total tissue loss from the blast; 2) adjacent areas with thermal and chemical burns; 3) distant sites with shrapnel-related wounds; 4) injuries arising from barotrauma. Results We identified 64 patients who were mostly male (84%), averaging 38 ± 14 years old. Injury mechanisms included fireworks (19%), industrial accidents (16%), volatile fuels and drug labs (45%), and others including can, battery, lighter explosions (20%). All mechanisms had equivalent frequency of Zone 2 injuries with an average TBSA of 17 ± 18%. Drug-related blasts caused the highest TBSA (34 ± 23%) and the most full-thickness burns (33% vs average 23%). Fireworks had over five times (17% vs. 3%) more Zone 3 and three times (25% vs 8%) more Zone 4 injuries compared to the other mechanisms. Upper extremities were involved at twice the rate of other body regions (43% vs 19%). Patients presenting to our burn team over 24 hours after initial injury had infections in 50% of cases – a four-fold increase compared to non-delayed presentations (50% vs 13%). Overall, 45% required surgery (32% grafting, 3% flaps) but 100% of the drug-related blasts needed surgical intervention. Some patients (58%) required ICU admission with the highest rate (83%) in the drug-related group. Conclusions Blast injuries most often required admission for management of the Zone 2 component. Each blast mechanism resulted in distinct distributions of injury although fireworks had the greatest number of Zone 1, 3, and 4 injuries. Firework blasts were often less severe and more likely to present delayed with infectious complications. Larger blast mechanisms including drug-related lab explosions as well as industrial blasts had the highest rates of ICU admission, TBSA, full thickness depth, upper extremity involvement, and need for surgical intervention.
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Hu, Zhijian, Jian Q. Fang, and Lizhi Z. Sun. "Blast effect zones and damage mechanisms of concrete bridges under above-deck car-bomb attacks." International Journal of Damage Mechanics 27, no. 8 (May 10, 2017): 1156–72. http://dx.doi.org/10.1177/1056789517708827.
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Above-deck car-bomb attacks cause significant effects on engineering structures from those of explosive blasts in the free air. In this paper, a post-disaster investigation for an existing bridge under above-deck car-bomb blast loading is presented. The crack distribution and damage deformation of the blast loaded girders are systematically investigated, and the blast effect zone is proposed to describe the blast loading distributions on girders and to characterize the girder structural behavior. The girder failure is dominated by one of the three typical damage mechanisms: shear, flexural-shear, and flexural, depending on the blast effect zone where the girder is located. The study also indicates that the isolation effect of the vehicle can significantly affect the blast loading distribution on structures, even though the isolation effect is localized in a region with a small standoff distance. It is gradually attenuated by the increase of the standoff distance. Numerical analysis is conducted to verify the findings by simulating the truck isolation effect with a steel plate.
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Ratcliff, Adam, Sam Rigby, Sam Clarke, and Stephen Fay. "A Review of Blast Loading in the Urban Environment." Applied Sciences 13, no. 9 (April 25, 2023): 5349. http://dx.doi.org/10.3390/app13095349.
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Urban blasts have become a significant concern in recent years. Whilst free-field blasts are well understood, the introduction of an urban setting (or any complex geometry) gives rise to multiple blast wave interactions and unique flow complexities, significantly increasing the difficulty of loading predictions. This review identifies commonly agreed-upon concepts or behaviours that are utilised to describe urban shock wave propagation, such as channelling and shielding, in conjunction with exploring urban characterisation metrics that aim to predict the effects on global blast loading for an urban blast. Likewise, discrepancies and contradictions are highlighted to promote key areas that require further work and clarification. Multiple numerical modelling programmes are acknowledged to showcase their ability to act as a means of validation and a preliminary testing tool. The findings contained within this review aim to inform future research decisions and topics better.
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Richmond, Donald R., E. Royce Fletcher, John T. Yelverton, and Yancy Y. Phillips. "Physical Correlates of Eardrum Rupture." Annals of Otology, Rhinology & Laryngology 98, no. 5_suppl (May 1989): 35–41. http://dx.doi.org/10.1177/00034894890980s507.
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Eardrum (tympanic membrane) rupture in humans and animals in relation to various blast pressure-time patterns was reviewed. There were few systematic studies on eardrum rupture as a consequence of blast overpressure. Most reports did not describe the area of the eardrum destroyed. The peak overpressures required to produce a 50% incidence of eardrum rupture (P50) were summarized. Most of the animal data pertained to dogs. The highest P50 for dogs, 296 kPa, was associated with smooth-rising overpressure. For complex wave patterns occurring inside open shelters subjected to nuclear blasts, the P50 was 205 kPa. For fast-rising blasts in a shock tube it was 78 kPa, and 105 kPa for statically applied pressures. The duration of the overpressure was not a factor unless it was very short. The influence of the orientation of the head to the oncoming blast was demonstrated. An ear facing the blast may receive reflected overpressures several times that for one side-on to the blast. An ear on the downstream side of the head was exposed to about the same overpressure as the side-on ear. A P50 for humans of 100 kPa and a threshold of 35 kPa has been used widely in blast criteria. A recent study suggests a threshold (P1) of about 20 kPa, and gives the overpressures required to produce minor, moderate, and major eardrum ruptures. These data were presented in the form of curves showing the overpressures as a function of duration required to inflict a P1 and a P50 of eardrum rupture of the three levels of severity.
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Kuendgen, Andrea, Corinna Strupp, Kathrin Nachtkamp, Barbara Hildebrandt, Rainer Haas, Norbert Gattermann, and Ulrich Germing. "Varying Importance of Prognostic Factors in Subgroups of Myelodysplastic Syndromes Defined by Blast Count, Cytogenetic Risk Group, or WHO Classification." Blood 114, no. 22 (November 20, 2009): 2776. http://dx.doi.org/10.1182/blood.v114.22.2776.2776.
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Abstract Abstract 2776 Poster Board II-752 Introduction: We wondered whether prognostic factors have similar relevance in different subpopulations of MDS patients. Methods: Our analysis was based on patients with primary, untreated MDS, including 181 RA, 169 RARS, 649 RCMD, 322 RSCMD, 79 5q-syndromes, 290 RAEB I, 324 RAEB II, 266 CMML I, 64 CMML II, and 209 RAEB-T. The impact of prognostic variables in univariate analysis was compared in subpopulations of patients defined by medullary blast count, namely <5%, ≥5% (table), ≥10%, and ≥20% (not shown), as well as 3 subpopulations defined by the cytogenetic risk groups according to IPSS (table). Multivariate analysis of prognostic factors was performed for cytogenetically defined subgroups and WHO-subtypes. Results: Strong prognostic factors in all blast-defined subgroups were hemoglobin, transfusion dependency, increased WBC, age, and LDH. However, all variables became less important in patients with ≥20% blasts (RAEB-T) and increased WBC was rare. Platelet count and cytogenetic risk groups were relevant in patients with <5%, ≥5%, and ≥10% marrow blasts, but not in RAEB-T. Marrow fibrosis was important in patients with <5% or ≥5% blasts, but not ≥10%. Gender and ANC <1000/μl were significant only in patients with a normal blast count. Furthermore, we looked for the effect of the karyotypes, relevant for IPSS scoring (-Y, del5q, del20q, others, del7q/-7, complex), and found a comparable influence on survival, irrespective whether patients had < or ≥5% marrow blasts. In subpopulations defined by cytogenetic risk groups, several prognostic factors were highly significant in univariate analysis, if patients had a good risk karyotype. These included hemoglobin, sex, age, LDH, increased WBC, transfusion need, and blast count (cut-offs 5%, 10%, and 20%). In the intermediate risk group only LDH, platelets, WBC, and blasts were significant prognostic factors, while in the high risk group only platelets and blast count remained significant. Multivariate analysis was performed for the cytogenetic risk groups and for subgroups defined by WHO subtypes. The analysis considered blast count (</≥5%), hemoglobin, platelets, ANC, cytogenetic risk group, transfusion need, sex, and age. In the subgroup including RA, RARS, and 5q-syndrome, LDH, transfusion, and age in descending order were independent prognostic parameters. In the RCMD+RSCMD group, karyotype, age, transfusion, and platelets were relevant factors. In the RAEB I+II subgroup, the order was hemoglobin, karyotype, age, and platelets, while in CMML I+II only hemoglobin had independent influence. In RAEB-T none of the factors examined was of independent significance. Looking at cytogenetic risk groups, in the favorable group, several variables independently influenced survival, namely transfusion, blasts, age, sex, and LDH (in this order). Interestingly, in the intermediate and high risk group, only blast count and platelets retained a significant impact. Conclusion: Univariate analysis showed prognostic factors (except ANC) included in IPSS and WPSS are relevant in most subgroups defined by marrow blast percentage. However, they all lose their impact if the blast count exceeds 20%. Regarding cytogenetic risk groups, several prognostic factors lose their influence already in the intermediate risk group. This underscores the prognostic importance of MDS cytogenetics. Multivariate analysis showed MDS subpopulations defined by WHO types also differ with regard to prognostic factors. In particular, CMML and RAEB-T stand out against the other MDS types. Disclosures: Kuendgen: Celgene: Honoraria. Hildebrandt:Celgene: Research Funding. Gattermann:Novartis: Honoraria, Participation in Advisory Boards on deferasirox clinical trials. Germing:Novartis, Celgene: Honoraria, Research Funding.
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Rafliansyah1, Muhammad, Irvani Irvani, and Haslen Oktarianty. "Analisis Penggunaan Powder Factor Terhadap Fragmentasi pada Lubang Ledak Vertikal dan Inclined di PT Aditya Buana Inter." MINERAL 4, no. 2 (October 31, 2019): 65–71. http://dx.doi.org/10.33019/mineral.v4i2.1597.
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Based on the observations in PT Aditya Buana Inter it is known that the vertical blast hole blasting geometry is an average of burden of 2.5 m, spacing 2.413 m, height 6 m, stemming 2.3 m, primary charge 4 m and diameter of 3 inch or 0.076 m blast hole, the amount of explosives used is in average 667 kg each blasts and the result of the volume rocks in average 2090,25 m3 or 5225,63 tons and has average powder factor value is 0.318kg/m3 with the average of fragmentation is 72.05 cm. Forthe inclined blast hole blasting geometry is an average of burden of 2.5 m, spacing 2.413 m, height 6.21 m, stemming 2.3 m, primary charge 4.21 m and diameter of 3 inch or 0.076 m blast hole, the amount of explosives used is in average 231 kg each blasts and the result of the volume rocks in average 718.44 m3 or 1796,1 tons and has average powder factor value is 0.321 kg/m3 with the average of fragmentation is 26,82 cm. The right of proposal design the blasting geometry in the field by calculating the inclined blast holes with 15° slope, using the evaluation blasting geometry actualobtained has modification blasting geometry with the good calculating which is burden 2.5 m, spacing 2.5 m, stemming 2.56 m, height level9.21 m, subdrilling 0.6 m, and primary charge length7.25 m.The result for a Powder Factor was more efficient which is 0.260 kg/m3 compared with the blasting geometry actual the vertical blast hole is 0.318 kg/m3 and the blasting geometry actual the inclined blast hole is 0.321 kg/m3, and the distribution of rock fragmentation resulting from blasting which corresponds to the target where rocks measuring> 100 cm to 0% compared to the actual geometry of vertical explosive holes of 29.6%.
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Pirooznia, Mehdi, Edward J. Perkins, and Youping Deng. "Batch Blast Extractor: an automated blastx parser application." BMC Genomics 9, Suppl 2 (2008): S10. http://dx.doi.org/10.1186/1471-2164-9-s2-s10.
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Depreter, Barbara, Mattias Hofmans, Eva Terras, Anne Uyttebroeck, An Van Damme, Alina Ferster, Jutte Van der Werff Ten Bosch, et al. "Deciphering Molecular Heterogeneity in Pediatric AML Using a Cancer Vs Normal Transcriptomic Approach." Blood 134, Supplement_1 (November 13, 2019): 1457. http://dx.doi.org/10.1182/blood-2019-125538.
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Introduction and Aim Although cytogenetics and response-guided therapy have considerably improved prognostication of pediatric AML (pedAML) patients, still 30-40% of the good responders relapse. Further delineation of the transcriptome of AML subpopulations, e.g. leukemic stem cells (LSCs), might result in a better understanding of pedAML biology and provide novel biomarkers for diagnostics, risk stratification, follow-up and targeted therapy. Methods Fluorescence-activated cell sorting (FACS) was used to isolate CD34+CD38- and CD34+CD38+ cells from pedAML patients/healthy controls (cord blood (CB), normal bone marrow (NBM)), defined as LSC/hematopoietic stem cell (HSC) and leukemic blast (L-blast)/control blast (C-blast), respectively. Sorting multiple phenotypes, both BM and blood, yielded 42 LSC and 35 L-blast fractions. Gene expression profiles (GEP) of LSCs and L-blasts were identified by a Cancer vs Normal (CvN) approach, whereas paired analysis of LSC vs L-blast aimed to identify LSC-specific aberrations. Micro-array analysis (4 pedAML, 3 CB) was followed by targeted quantitative PCR (qPCR) validation of the highest differentially expressed genes (DEGs) in LSC (n=52), L-blast (n=42) and between LSC and L-blast (n=15) (25 pedAML, 11 CB/9 NBM). DEGs were functionally analysed by protein association (STRING) and by gene set enrichment analysis (GSEA-Cytoscape). An overview of the workflow is shown in Fig. 1A. Results LSC vs HSC micro-array analysis revealed 83 up- and 212 downregulated targets (Fig. 1B). qPCR confirmed 8 and 11 of the 52 tested targets to be highly significantly up- and downregulated, respectively, in LSC (n=42) compared to HSC (n=20) (P<.001). Overexpressed targets contained well-known oncogenes (e.g. CFD, ANXA2, EMP1), next to genes with undefined roles in AML (e.g. PLIN2, CRIP1). Six out of the top 30 downregulated DEGs were described as tumor suppressor genes (TSGs) in solid tumors. Functional protein associations showed enriched cancer pathways, osteoclast differentiation, apoptosis and breast cancer, whereas Th17 cell differentiation and Rap1/MAPK signaling were suppressed in LSCs. LSC-enriched gene sets included inflammation, apoptosis, immune suppression and adipogenesis, while HSC-related signatures were anti-correlated. L-blasts showed 157 and 332 up- and downregulated DEGs (Fig. 1C), and 8/42 were confirmed as significantly upregulated by qPCR (L-blast=35 vs C-blast=19, P<.001) e.g. DUSP6, HOMER3 and EMP1. Functional analysis showed L-blasts to be associated with increased apoptosis and oxidative stress (FoxO signaling, cytokine-cytokine receptor interactions) compared to their normal counterparts. Gene sets enriched in LSCs vs L-blasts addressed inflammatory responses, adipogenesis, TNF signaling and response. Pathway analysis showed repression of cell cycle genes, consistent with LSC quiescence. qPCR validation of 15 out of the 117 upregulated targets (Fig. 1D), of which 5/15 had a neural link (PCDHB2, GPRIN3, SLC22A23, CDR1, RPGRIP1L), did not confirm significant dysregulated expression (P>.05). Interestingly, the set of DEGs between LSCs and L-blasts shared only few genes with those differentially expressed between HSC and C-blast (28/306; 4 up- and 24 downregulated). This low intersection (7.7%) is in strong contrast to the previously reported 34% in adult AML (Gal et al. 2006). Moreover, none of those genes were detected in adult AML (except for CD38 downregulation). Remarkably, 3/4 mutual upregulated genes (IGF2, GPRIN3, PROS1, CYTH4) are involved in neural crossroads and have no relation to stemness nor AML. Conclusion Combining FACS with CvN transcriptomic profiling, followed by qPCR validation, identified novel DEGs in pedAML subpopulations. The majority of the most significant upregulated targets in LSCs (n=8) and L-blasts (n=8) showed no previous link to pedAML. Identification of 11 novel downregulated targets, often described as TSGs in solid tumors, warrants further studies whether hypomethylating therapy could result into LSC eradication in pedAML. LSCs reflected low cell cycle activity and elevated inflammatory response, hypoxia, metabolic dysregulation and signaling compared to HSC. GEP of LSCs vs L-blasts revealed a distinct molecular landscape compared to adult AML, and suggested a possible link between distortion of neural and hematopoietic systems in leukemogenesis. Figure Disclosures No relevant conflicts of interest to declare.