Academic literature on the topic 'Biophysical characterisation'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Biophysical characterisation.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Biophysical characterisation"

1

Nestorow, Stephanie A., Tim R. Dafforn, and Verna Frasca. "Biophysical characterisation of SMALPs." Biochemical Society Transactions 49, no. 5 (October 13, 2021): 2037–50. http://dx.doi.org/10.1042/bst20201088.

Full text
Abstract:
Membrane proteins such as receptors, ion channels and transport proteins are important drug targets. The structure-based study of membrane proteins is challenging, especially when the target protein contains both soluble and insoluble domains. Most membrane proteins are insoluble in aqueous solvent and embedded in the plasma membrane lipid bilayer, which significantly complicates biophysical studies. Poly(styrene-co-maleic acid) (SMA) and other polymer derivatives are increasingly common solubilisation agents, used to isolate membrane proteins stabilised in their native lipid environment in the total absence of detergent. Since the initial report of SMA-mediated solubilisation, and the formation of SMA lipid particles (SMALPs), this technique can directly isolate therapeutic targets from biological membranes, including G-protein coupled receptors (GPCRs). SMA now allows biophysical and structural analyses of membrane proteins in solution that was not previously possible. Here, we critically review several existing biophysical techniques compatible with SMALPs, with a focus on hydrodynamic analysis, microcalorimetric analysis and optical spectroscopic techniques.
APA, Harvard, Vancouver, ISO, and other styles
2

Khalesi, Mohammadreza, Nathalie Mandelings, Zahra Shokribousjein, David Riveros-Galan, Hubert Verachtert, Kurt Gebruers, Frank Delvigne, Ivo Vankelecom, and Guy Derdelinckx. "Biophysical characterisation of hydrophobin enriched foamate." Cerevisia 38, no. 4 (January 2014): 129–34. http://dx.doi.org/10.1016/j.cervis.2014.04.003.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Picciotto, Sabrina, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, et al. "Isolation of extracellular vesicles from microalgae: towards the production of sustainable and natural nanocarriers of bioactive compounds." Biomaterials Science 9, no. 8 (2021): 2917–30. http://dx.doi.org/10.1039/d0bm01696a.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Haar, G. ter, F. Duck, H. Starritt, and S. Daniels. "Biophysical characterisation of diagnostic ultrasound equipment-preliminary results." Physics in Medicine and Biology 34, no. 11 (November 1, 1989): 1533–42. http://dx.doi.org/10.1088/0031-9155/34/11/002.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Plesner, Bitten, Peter Westh, and Anders D. Nielsen. "Biophysical characterisation of GlycoPEGylated recombinant human factor VIIa." International Journal of Pharmaceutics 406, no. 1-2 (March 15, 2011): 62–68. http://dx.doi.org/10.1016/j.ijpharm.2010.12.034.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Castro, Ignacio Hugo, Alejandro Ferrari, María Georgina Herrera, Martín Ezequiel Noguera, Lorenzo Maso, Monica Benini, Alessandra Rufini, Roberto Testi, Paola Costantini, and Javier Santos. "Biophysical characterisation of the recombinant human frataxin precursor." FEBS Open Bio 8, no. 3 (January 25, 2018): 390–405. http://dx.doi.org/10.1002/2211-5463.12376.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Scheidt, Tom, Tadas Kartanas, Quentin Peter, Matthias M. Schneider, Kadi L. Saar, Thomas Müller, Pavan Kumar Challa, Aviad Levin, Sean Devenish, and Tuomas P. J. Knowles. "Multidimensional protein characterisation using microfluidic post-column analysis." Lab on a Chip 20, no. 15 (2020): 2663–73. http://dx.doi.org/10.1039/d0lc00219d.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Kwan, Tristan O. C., Rosana Reis, Giuliano Siligardi, Rohanah Hussain, Harish Cheruvara, and Isabel Moraes. "Selection of Biophysical Methods for Characterisation of Membrane Proteins." International Journal of Molecular Sciences 20, no. 10 (May 27, 2019): 2605. http://dx.doi.org/10.3390/ijms20102605.

Full text
Abstract:
Over the years, there have been many developments and advances in the field of integral membrane protein research. As important pharmaceutical targets, it is paramount to understand the mechanisms of action that govern their structure–function relationships. However, the study of integral membrane proteins is still incredibly challenging, mostly due to their low expression and instability once extracted from the native biological membrane. Nevertheless, milligrams of pure, stable, and functional protein are always required for biochemical and structural studies. Many modern biophysical tools are available today that provide critical information regarding to the characterisation and behaviour of integral membrane proteins in solution. These biophysical approaches play an important role in both basic research and in early-stage drug discovery processes. In this review, it is not our objective to present a comprehensive list of all existing biophysical methods, but a selection of the most useful and easily applied to basic integral membrane protein research.
APA, Harvard, Vancouver, ISO, and other styles
9

Kasim, Johanes K., Iman Kavianinia, Jin Ng, Paul W. R. Harris, Nigel P. Birch, and Margaret A. Brimble. "Efficient synthesis and characterisation of the amyloid beta peptide, Aβ1–42, using a double linker system." Organic & Biomolecular Chemistry 17, no. 1 (2019): 30–34. http://dx.doi.org/10.1039/c8ob02929f.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Reis, Ana, Joana P. F. Teixeira, Ana M. G. Silva, Mariana Ferreira, Paula Gameiro, and Victor de Freitas. "Modelling Hyperglycaemia in an Epithelial Membrane Model: Biophysical Characterisation." Biomolecules 12, no. 10 (October 21, 2022): 1534. http://dx.doi.org/10.3390/biom12101534.

Full text
Abstract:
Biomimetic models are valuable platforms to improve our knowledge on the molecular mechanisms governing membrane-driven processes in (patho)physiological conditions, including membrane permeability, transport, and fusion. However, current membrane models are over simplistic and do not include the membrane’s lipid remodelling in response to extracellular stimuli. Our study describes the synthesis of glycated dimyristoyl-phosphatidylethanolamine (DMPE-glyc), which was structurally characterised by mass spectrometry (ESI-MS) and quantified by NMR spectroscopy to be further incorporated in a complex phospholipid (PL) membrane model enriched in cholesterol (Chol) and (glyco)sphingolipids (GSL) designed to mimic epithelial membranes (PL/Chol/GSL) under hyperglycaemia conditions. Characterisation of synthesised DMPE-glyc adducts by tandem mass spectrometry (ESI-MS/MS) show that synthetic DMPE-glyc adducts correspond to Amadori products and quantification by 1H NMR spectroscopy show that the yield of glycation reaction was 8%. The biophysical characterisation of the epithelial membrane model shows that excess glucose alters the thermotropic behaviour and fluidity of epithelial membrane models likely to impact permeability of solutes. The epithelial membrane models developed to mimic normo- and hyperglycaemic scenarios are the basis to investigate (poly)phenol-lipid and drug–membrane interactions crucial in nutrition, pharmaceutics, structural biochemistry, and medicinal chemistry.
APA, Harvard, Vancouver, ISO, and other styles

Dissertations / Theses on the topic "Biophysical characterisation"

1

Sarkar, Supti. "Biophysical characterisation of plasmid formulation." Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1446862/.

Full text
Abstract:
DNA vaccination and gene therapy offer significant advantages in the treatment of many intractable diseases but many technical challenges must be overcome before the potential of these techniques are realised. A major challenge for non-viral vector particles is their physical instability in physiological conditions. The delivery vector investigated was a Lipid-Peptide-DNA (LPD) complex. The initial system was composed of lipofectin, peptide 6 and a 6.9 kb plasmid DNA. The aim of this work was to study parameters with a view of optimising the biophysical characteristics of the system, namely with regard to storage and transportation. The effects of ionic strength and pH on colloidal stability and structure of the gene delivery vector were investigated using dynamic and static light scattering. Results showed increased levels of aggregation at physiological concentrations (150mM NaCl), although a more stable system was observed in distilled water and at extreme sah conditions of IM. The rates of aggregation were found to be related to the zeta potential of the system and could be predicted using Monte Carlo simulations. Fractal dimension of complexes (where higher values correspond to more compact structures) showed values of 1.6 for 150 mM NaCl and 2.4 for IM NaCl. Lipids, peptides and plasmid DNA were investigated to see the effect of chemistry and physical structure on the aggregation properties of the system. Lipid studies assessing the release of fluorescent calcein from a variety of lipids, over a range of pH found Cl4 Unsaturated + DOPE had the greatest level of release when observed at pH 5.0. The effect of increasing plasmid DNA size (number of base pairs 5.7-72) was also investigated, larger plasmid sizes showed greater stability although further investigation is required to confirm this.
APA, Harvard, Vancouver, ISO, and other styles
2

Damianoglou, Angeliki. "Biophysical characterisation of peptides and proteins." Thesis, University of Warwick, 2010. http://wrap.warwick.ac.uk/3664/.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Venkatesan, Meenakshi. "Biophysical characterisation of PAK/Cdc42 interaction." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620265.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Tsytlonok, Maksym. "Biophysical characterisation of linear repeat proteins." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610764.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Herbert, Andrew Peter. "Biophysical characterisation of measles virus receptors." Thesis, University of Edinburgh, 2002. http://hdl.handle.net/1842/15013.

Full text
Abstract:
To date two human proteins have been identified that can serve as the receptor for measles virus, namely membrane cofactor protein (MCP) and signalling lymphocyte activation molecule (SLAM). It is thought that a more through understanding of the structure and behaviour of the interacting regions of these receptors, will facilitate a more detailed understanding of the pathogenesis of MV. This thesis describes efforts to clone, express and characterise the relevant fragments of these receptors. MCP carries a functionally critical N-glycan on its second module (MCP2) and the crystal structure of MCP12 (expressed in mammalian cells) implies a structural role for this carbohydrate moiety. MCP12 and MCP2 were successfully cloned and expressed as secreted proteins in Pichia pastoris at levels sufficient for biophysical characterisation. Proton and 15N Nuclear magnetic resonance spectroscopy (NMR) studies, differential scanning calorimetry and mass spectrometry were employed to investigate the recombinant proteins. Both the natural sequences of MCP12 and MCP2 fragments expressed in P. pastoris were hyperglycosylated and therefore these recombinant fragments were investigated in both glycosylated and deglycolylated forms in order to study the effect of the glycans on module 2 of MCP, and with a view to detailed structural and dynamic studies using NMR. The data obtained with the P. pastoris-expressed fragments are not inconsistent with the hypothesis that the N-glycan on module 2 of the wild-type MCP is required for this molecule to be properly folded.  After extensive investigations, it was concluded that the inability of P. pastoris to attach the natural glycan is a barrier to more detailed structural studies. In an effort to produce MCP2 with a better defined glycosylation profile, efforts were made to express, this fragment in insect cells which are known to produce a ‘more natural’ glycosylation profile on proteins. Moreover, the MCP2 fragment was also expressed in E. coli, in order to produce a form of MCP2 that had never been glycosylated.
APA, Harvard, Vancouver, ISO, and other styles
6

Tully, Mark David. "Biophysical characterisation of SAP97 and AKAP79." Thesis, University of Liverpool, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.548812.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Clewes, Oliver. "Recombinant proneurotrophins : production, purification and biophysical characterisation." Thesis, University of Bristol, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.443699.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Philippe, Didier. "Biophysical characterisation of 'Staphylococcus aureus' defence proteins." Thesis, University of Bath, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.426285.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Chen, Ho Ann. "Biophysical characterisation of rat CD2 domain 1." Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.392332.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Harvey, Richard Douglas. "Biophysical characterisation of vesicles containing nonionic surfactants." Thesis, King's College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.396320.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Books on the topic "Biophysical characterisation"

1

Streeter, Simon David. Biochemical and biophysical characterisation of the gene regulatory protein C.AhdI. Portsmouth: University of Portsmouth, School of Biological Sciences, 2004.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
2

Fox, Daniel George. Expression and biophysical characterisation of the domains of the Pf1 gene 5 protein. Portsmouth: University of Portsmouth, School of Biological Sciences, 1994.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
3

Smith, Melanie A. Biochemical and biophysical characterisation of the domain structure of the HsdS subunit of EcoR124I. Portsmouth: University of Portsmouth, Institute of Biomedical and Biomolecular Sciences, 2000.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
4

Plyte, Simon Edward. The biochemical and biophysical characterisation of the Pf1 gene 5 protein and its complex with nucleic acids. Portsmouth: Portsmouth Polytechnic, School of Biological Sciences, 1990.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

Limbert, Georges. Skin Biophysics: From Experimental Characterisation to Advanced Modelling. Springer International Publishing AG, 2020.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
6

Limbert, Georges. Skin Biophysics: From Experimental Characterisation to Advanced Modelling. Springer, 2019.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

Maltsev, Valeri P., and Konstantin A. Semyanov. Characterisation of Bio-Particles from Light Scattering. de Gruyter GmbH, Walter, 2013.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
8

Maltsev, V. P., and K. A. Semyanov. Characterisation Of Bio-particles From Light Scattering (Inverse and Ill-Posed Problems). Brill Academic Publishers, 2004.

Find full text
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Biophysical characterisation"

1

Piégay, Hervé, Adrien Alber, J. Wesley Lauer, Anne-Julia Rollet, and Elise Wiederkehr. "Biophysical Characterisation of Fluvial Corridors at Reach to Network Scales." In Fluvial Remote Sensing for Science and Management, 241–69. Chichester, UK: John Wiley & Sons, Ltd, 2012. http://dx.doi.org/10.1002/9781119940791.ch11.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Tinker-Mill, Claire Louisa. "The Application of Biophysical Techniques to the Study of the Inhibition of Aggregation of Aβ Using PINPs Liposomes." In Nanoscale Imaging and Characterisation of Amyloid-β, 121–37. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-39534-0_8.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Onyango, J. W., A. O. Esilaba, and P. K. Kimani. "Stakeholder Characterisation of the Biophysical and Socio-economic Potential of the Desert Margins in Kenya." In Innovations as Key to the Green Revolution in Africa, 935–45. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-90-481-2543-2_94.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Guzik, Malgorzata, E. Tomaszewicz, Yannick Guyot, P. Tomaszewicz, J. Legendziewicz, and George Boulon. "Characterisations of New Nd3+-Doped Scheelite-Type Molybdates for Laser Materials." In NATO Science for Peace and Security Series B: Physics and Biophysics, 503–4. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9133-5_45.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Omar, Hamdan. "Multiple Sensors and Platforms for Biophysical and Biochemical Characterisations of Various Ecosystem Types of Tropical Forests in Malaysia: Advance, Limitation, and Opportunity." In Concepts and Applications of Remote Sensing in Forestry, 27–48. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-4200-6_2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Miles, A. J., and B. A. Wallace. "Biopharmaceutical applications of protein characterisation by circular dichroism spectroscopy." In Biophysical Characterization of Proteins in Developing Biopharmaceuticals, 123–52. Elsevier, 2020. http://dx.doi.org/10.1016/b978-0-444-64173-1.00006-8.

Full text
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography