Academic literature on the topic 'Biomaterials, regenerative medicine, carbohydrates, proteins'
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Journal articles on the topic "Biomaterials, regenerative medicine, carbohydrates, proteins"
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Fernández-Villa, Daniel, Mirta Jiménez Gómez-Lavín, Cristina Abradelo, Julio San Román, and Luis Rojo. "Tissue Engineering Therapies Based on Folic Acid and Other Vitamin B Derivatives. Functional Mechanisms and Current Applications in Regenerative Medicine." International Journal of Molecular Sciences 19, no. 12 (December 16, 2018): 4068. http://dx.doi.org/10.3390/ijms19124068.
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B-vitamins are a group of soluble vitamins which are cofactors of some of the enzymes involved in the metabolic pathways of carbohydrates, fats and proteins. These compounds participate in a number of functions as cardiovascular, brain or nervous systems. Folic acid is described as an accessible and multifunctional niche component that can be used safely, even combined with other compounds, which gives it high versatility. Also, due to its non-toxicity and great stability, folic acid has attracted much attention from researchers in the biomedical and bioengineering area, with an increasing number of works directed at using folic acid and its derivatives in tissue engineering therapies as well as regenerative medicine. Thus, this review provides an updated discussion about the most relevant advances achieved during the last five years, where folic acid and other vitamins B have been used as key bioactive compounds for enhancing the effectiveness of biomaterials’ performance and biological functions for the regeneration of tissues and organs.
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Nag, Kakon, and Toshihiro Akaike. "E-Cadherin – Fc Chimeric Protein-Based Biomaterial: Breaking the Barriers in Stem Cell Technology and Regenerative Medicine." Advanced Materials Research 810 (September 2013): 41–76. http://dx.doi.org/10.4028/www.scientific.net/amr.810.41.
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Chimeric proteins have been used for years for various purposes ranging from biomaterials to candidate drug molecules, and from bench to bulk. Regenerative medicine needs various kinds of proteins for providing essential factors for maintaining starting cells, like induced pluripotent stem cells (iPSC), and renewal, proliferation, targeted differentiation of these cells, and as extracellular matrix for the experimental cells. However, there are several challenges associated with making functional chimeric proteins for effective application as biomaterial in this field. Fc-chimeric protein technology could be an effective solution to overcome many of them. These tailored proteins are recently becoming superior choice of biomaterials in stem cell technology and regenerative medicine due to their specific advantageous biophysical and biochemical properties over other chimeric forms of same proteins. Recent advances in recombinant protein-related science and technology also expedited the popularity of this kind of engineered protein. Over the last decade our lab has been pioneering this field, and we and others have been successfully applied Fc-chimeric proteins to overcome many critical issues in stem cell technologies targeting regenerative medicine and tissue engineering. Fc-chimeric protein-based biomaterials, specifically, E-cad-Fc have been preferentially applied for coating of cell culture plates for establishing xenogeneic-agent free monolayer stem cell culture and their maintenance, enhanced directed differentiation of stem cells to specific lineages, and non-enzymatic on-site one-step purification of target cells. Here the technology, recent discoveries, and future direction related with the E-cad-Fc-chimeric protein in connection with regenerative medicine are described.
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Jahangirian, Azizi, Rafiee-Moghaddam, Baratvand, and Webster. "Status of Plant Protein-Based Green Scaffolds for Regenerative Medicine Applications." Biomolecules 9, no. 10 (October 17, 2019): 619. http://dx.doi.org/10.3390/biom9100619.
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In recent decades, regenerative medicine has merited substantial attention from scientific and research communities. One of the essential requirements for this new strategy in medicine is the production of biocompatible and biodegradable scaffolds with desirable geometric structures and mechanical properties. Despite such promise, it appears that regenerative medicine is the last field to embrace green, or environmentally-friendly, processes, as many traditional tissue engineering materials employ toxic solvents and polymers that are clearly not environmentally friendly. Scaffolds fabricated from plant proteins (for example, zein, soy protein, and wheat gluten), possess proper mechanical properties, remarkable biocompatibility and aqueous stability which make them appropriate green biomaterials for regenerative medicine applications. The use of plant-derived proteins in regenerative medicine has been especially inspired by green medicine, which is the use of environmentally friendly materials in medicine. In the current review paper, the literature is reviewed and summarized for the applicability of plant proteins as biopolymer materials for several green regenerative medicine and tissue engineering applications.
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Torres-Huerta, Ana Laura, Aurora Antonio-Pérez, Yolanda García-Huante, Nayelhi Julieta Alcázar-Ramírez, and Juan Carlos Rueda-Silva. "Biomolecule-Based Optical Metamaterials: Design and Applications." Biosensors 12, no. 11 (November 2, 2022): 962. http://dx.doi.org/10.3390/bios12110962.
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Metamaterials are broadly defined as artificial, electromagnetically homogeneous structures that exhibit unusual physical properties that are not present in nature. They possess extraordinary capabilities to bend electromagnetic waves. Their size, shape and composition can be engineered to modify their characteristics, such as iridescence, color shift, absorbance at different wavelengths, etc., and harness them as biosensors. Metamaterial construction from biological sources such as carbohydrates, proteins and nucleic acids represents a low-cost alternative, rendering high quantities and yields. In addition, the malleability of these biomaterials makes it possible to fabricate an endless number of structured materials such as composited nanoparticles, biofilms, nanofibers, quantum dots, and many others, with very specific, invaluable and tremendously useful optical characteristics. The intrinsic characteristics observed in biomaterials make them suitable for biomedical applications. This review addresses the optical characteristics of metamaterials obtained from the major macromolecules found in nature: carbohydrates, proteins and DNA, highlighting their biosensor field use, and pointing out their physical properties and production paths.
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Filipczak, Nina, Satya Siva Kishan Yalamarty, Xiang Li, Muhammad Muzamil Khan, Farzana Parveen, and Vladimir Torchilin. "Lipid-Based Drug Delivery Systems in Regenerative Medicine." Materials 14, no. 18 (September 17, 2021): 5371. http://dx.doi.org/10.3390/ma14185371.
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The most important goal of regenerative medicine is to repair, restore, and regenerate tissues and organs that have been damaged as a result of an injury, congenital defect or disease, as well as reversing the aging process of the body by utilizing its natural healing potential. Regenerative medicine utilizes products of cell therapy, as well as biomedical or tissue engineering, and is a huge field for development. In regenerative medicine, stem cells and growth factor are mainly used; thus, innovative drug delivery technologies are being studied for improved delivery. Drug delivery systems offer the protection of therapeutic proteins and peptides against proteolytic degradation where controlled delivery is achievable. Similarly, the delivery systems in combination with stem cells offer improvement of cell survival, differentiation, and engraftment. The present review summarizes the significance of biomaterials in tissue engineering and the importance of colloidal drug delivery systems in providing cells with a local environment that enables them to proliferate and differentiate efficiently, resulting in successful tissue regeneration.
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Kishan Shetty, Ashmitha, Serene Joy, Manasa Latha Biligowda, and Siddique Sha Muhammed Hussain. "Biomarkers of Pulpal Regeneration: Overview on Immunohistochemistry Analysis." ECS Transactions 107, no. 1 (April 24, 2022): 17193–99. http://dx.doi.org/10.1149/10701.17193ecst.
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Immunocytochemistry (IHC) is a method that uses monoclonal and polyclonal antibodies to determine the tissue distribution of an antigen of interest in health and disease. The method of recognizing a tissue component in situ utilizing unique antibody-antigen interactions in which the antibody is precisely labelled is referred to as IHC. It can be used to identify and localize well-known cellular structures and also extracellular matrix components. This method similarly provides information on the temporospatial distribution of newly discovered carbohydrates and proteins in development, illness and health. Endodontics can use IHC as a diagnostic tool and a potential marker for odontogenic tissues. Although histopathologic examination of periapical lesions reveals the true nature of regenerative tissue, immunohistochemical markers can be utilized to refine the tissue's composition.
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Khosropanah, Mohammad Hossein, Mahdi Alizadeh Vaghasloo, Mehdi Shakibaei, Anna‐Lena Mueller, Abdol‐Mohammad Kajbafzadeh, Leila Amani, Ismaeil Haririan, Ashkan Azimzadeh, Zahra Hassannejad, and Masoumeh Majidi Zolbin. "Biomedical applications of silkworm ( Bombyx Mori ) proteins in regenerative medicine (a narrative review)." Journal of Tissue Engineering and Regenerative Medicine 16, no. 2 (December 7, 2021): 91–109. http://dx.doi.org/10.1002/term.3267.
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Lee, Jung-Hwan, Ji-Young Yoon, Jun Hee Lee, Hae-Hyoung Lee, Jonathan C. Knowles, and Hae-Won Kim. "Emerging biogenesis technologies of extracellular vesicles for tissue regenerative therapeutics." Journal of Tissue Engineering 12 (January 2021): 204173142110190. http://dx.doi.org/10.1177/20417314211019015.
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Extracellular vesicles (EVs), including exosomes, carry the genetic packages of RNA, DNA, and proteins and are heavily involved in cell-cell communications and intracellular signalings. Therefore, EVs are spotlighted as therapeutic mediators for the treatment of injured and dysfunctional tissues as well as biomarkers for the detection of disease status and progress. Several key issues in EVs, including payload content and bioactivity, targeting and bio-imaging ability, and mass-production, need to be improved to enable effective therapeutics and clinical translation. For this, significant efforts have been made recently, including genetic modification, biomolecular and chemical treatment, application of physical/mechanical cues, and 3D cultures. Here we communicate those recent technological advances made mainly in the biogenesis process of EVs or at post-collection stages, which ultimately aimed to improve the therapeutic efficacy in tissue healing and disease curing and the possibility of clinical translation. This communication will help tissue engineers and biomaterial scientists design and produce EVs optimally for tissue regenerative therapeutics.
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Vacchini, Mattia, Rana Edwards, Roberto Guizzardi, Alessandro Palmioli, Carlotta Ciaramelli, Alice Paiotta, Cristina Airoldi, Barbara La Ferla, and Laura Cipolla. "Glycan Carriers As Glycotools for Medicinal Chemistry Applications." Current Medicinal Chemistry 26, no. 35 (December 13, 2019): 6349–98. http://dx.doi.org/10.2174/0929867326666190104164653.
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Carbohydrates are one of the most powerful and versatile classes of biomolecules that nature uses to regulate organisms’ biochemistry, modulating plenty of signaling events within cells, triggering a plethora of physiological and pathological cellular behaviors. In this framework, glycan carrier systems or carbohydrate-decorated materials constitute interesting and relevant tools for medicinal chemistry applications. In the last few decades, efforts have been focused, among others, on the development of multivalent glycoconjugates, biosensors, glycoarrays, carbohydrate-decorated biomaterials for regenerative medicine, and glyconanoparticles. This review aims to provide the reader with a general overview of the different carbohydrate carrier systems that have been developed as tools in different medicinal chemistry approaches relying on carbohydrate-protein interactions. Given the extent of this topic, the present review will focus on selected examples that highlight the advancements and potentialities offered by this specific area of research, rather than being an exhaustive literature survey of any specific glyco-functionalized system.
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Miyata, T., and K. Kurokawa. "Carbonyl Stress: Increased Carbonyl Modification of Proteins by Autoxidation Products of Carbohydrates and Lipids in Uremia." International Journal of Artificial Organs 22, no. 4 (April 1999): 195–98. http://dx.doi.org/10.1177/039139889902200402.
Full textDissertations / Theses on the topic "Biomaterials, regenerative medicine, carbohydrates, proteins"
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SGAMBATO, ANTONELLA. "New nanostructured biomaterials for regenerative medicine." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2016. http://hdl.handle.net/10281/102470.
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Innovative approaches in tissue engineering and regenerative medicine based on decellularized extracellular matrix (ECM) scaffolds and tissues are quickly growing. ECM proteins are particularly adequate toward tissue regeneration applications, since they are natural biomaterials that can be bio-activated with signalling molecules able to influence cell fate, driving cell responses and tissue regeneration. Indeed, it is well recognized that cells perceive and respond to their microenvironment; the underlying mechanisms are generally complex and sometimes still poorly understood. Carbohydrates, found as complex polysaccharides or conjugated to other structural and functional proteins, are relevant components of the cell environment and cell membrane, contributing to cell interactions at several levels: for example, proteoglycans are a major constituent of the extracellular matrix (ECM) surrounding a cell, and glycosoaminoglycans (GAGs) participate in cell-ECM interactions and mediate cell-cell communications. It is now well recognized that glycans play an essential role in a plethora of biological processes, including cellular adhesion, migration, and differentiation, disease progression, and modulation of immunological responses. Although this relevant role, carbohydrates have been rarely considered as signalling cues for ECM derived scaffold functionalization and activation, due to their complexity in synthetic manipulations. Nevertheless, recent data highlight that they can be promising tools for tissue engineering and regenerative medicine applications.
Collagen and elastin, in form of 2D matrices or in their hydrolized forms have been bioactivated with different glycidic epitopes; characterizations and biological evaluations have been made. In particular this neo-glycosylated biomaterials have been tested for their ability to influence cell fate; we found that glucose-functionalized biomaterials are able to drive neuronal differentiation, and sialic acid, depending on the regiochemistry of its glycosidic bond, drives mesenchymal stem cells to differentiate in osteogenic or chondrogenic direction.
Inspired by the aggrecan, a natural proteoglycans found in cartilaginous tissues, with a bottlebrush structure, another work has been based on the design and production of a synthetic macromolecule, composed of collagen, as core protein, modified with the natural glycosaminoglycan chondroitin sulfate. Due to the high morbidity of some cartilage and bone diseases, and the difficulty, or impossibility, to restore ailing joints, the synthesis of these macromolecules is interesting and could be useful in cartilage tissue regeneration.
The area of hydrogels as biomaterials has also been taken into account. Hydrogels are three-dimensional hydrophilic polymer networks obtained from synthetic and/or natural polymers. They are able to swell and retain a large portion of water when placed in an aqueous solution. We synthesized hydrogels, by using modified gelatin with different functional groups, or gelatin in combination with cross-linking agents. Hydrogels have become increasingly studied as matrices for tissue engineering. This kind of material are able to guarantee a 3D environment for cell culture.
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Leonard, Alex. "Elastin Like Polypeptides as Drug Delivery Vehicles in Regenerative Medicine Applications." Scholar Commons, 2016. http://scholarcommons.usf.edu/etd/5981.
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Elastin like polypeptides (ELPs) are a class of naturally derived biomaterials that are non-immunogenic, genetically encodable, and biocompatible making them ideal for a variety of biomedical applications, ranging from drug delivery to tissue engineering. Also, ELPs undergo temperature-mediated inverse phase transitioning, which allows them to be purified in a relatively simple manner from bacterial expression hosts. Being able to genetically encode ELPs allows for the incorporation of bioactive peptides and functionalization of ELPs. This work utilizes ELPs for regenerative medicine and drug delivery.
The goal of the first study was to synthesize a biologically active epidermal growth factor-ELP (EGF-ELP) fusion protein that could aid in the treatment of chronic wounds. EGF plays a crucial role in wound healing by inducing epithelial cell proliferation and migration, and fibroblast proliferation. The use of exogenous EGF has seen success in the treatment of acute wounds, but has seen relatively minimal success in chronic wounds because the method of delivery does not protect exogenous EGF from degradation, or prevent it from diffusing away from the application site.
We created an EGF-ELP fusion protein to combat these issues. As demonstrated through the proliferation of human skin fibroblasts in vitro, the EGF-ELP may be able to aid in the treatment of chronic wounds. Furthermore, the ability of the EGF-ELP to self-assemble near physiological temperatures could allow for the formation of drug depots at the wound site and minimize diffusion, increasing the bioavailability of EGF and enhancing tissue regeneration.
The objective of the second study was to create an injectable hydrogel platform that does not require conjugation of functional moieties for crosslinking or biological activity. Hydrogels are three-dimensional polymer networks that are able to absorb water and biological fluids without dissolving. Their high water content gives them physical properties similar to soft tissues, making them useful as scaffolds for cell migration and drug delivery vehicles. Injectable hydrogels that crosslink in situ are particularly useful because they can form to the shape of the defect, providing a near perfect fit. However, many hydrogel platforms cannot be crosslinked in situ because cytotoxic crosslinking reagents are required. Additionally, hydrogels typically require the chemical conjugation of crosslinking domains and bioactive peptides to the polymer backbone, adding more steps and time required for hydrogel production.
We devised an injectable hydrogel platform that can be synthesized in a single step using photoreactive ELPs as the polymer backbone. Leucine auxotrophic Eshcherichia coli expressed ELPs containing photoleucine, a leucine analog and photoreactive diazirine crosslinker, which is substituted for leucine periodically throughout the ELP sequence. Upon exposure to ultraviolet radiation (~370 nm), photoleucine is able to form covalent crosslinks with amino acid side chains, forming a polymer network for hydrogel formation. Additionally, recombinant growth factors and morphogens can be encoded into the ELP sequence providing a simple method of hydrogel functionalization for regenerative medicine applications.
The potential for this platform was demonstrated through in vivo crosslinking of photoreactive ELPs in the expression hosts. Though the production of the photoreactive ELP was not as forthright as originally assumed. The substitution of noncanonical amino acids typically requires the auxotrophic expression hosts to be starved of the amino acid that they are auxotrophic for. A noncanonical analog of said amino acid can then be supplemented into expression media, maximizing incorporation. In this investigation, it was found the addition of photoleucine alone inhibited photoreactive ELP expression. ELP expression only occurred in the presence of photoleucine if valine or leucine was also present in the media. Furthermore, valine was found to aid the production of ELPs as much as leucine. It was postulated the bacterial translational machinery might need to be altered for optimal ELP expression.
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Grasman, Jonathan M. "Designing Fibrin Microthread Scaffolds for Skeletal Muscle Regeneration." Digital WPI, 2015. https://digitalcommons.wpi.edu/etd-dissertations/18.
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Volumetric muscle loss (VML) typically results from traumatic incidents; such as those presented from combat missions, where soft-tissue extremity injuries account for approximately 63% of diagnoses. These injuries lead to a devastating loss of function due to the complete destruction of large amounts of tissue and its native basement membrane, removing important biochemical cues such as hepatocyte growth factor (HGF), which initiates endogenous muscle regeneration by recruiting progenitor cells. Clinical strategies to treat these injuries consist of autologous tissue transfer techniques, requiring large amounts of healthy donor tissue and extensive surgical procedures that can result in donor site morbidity and limited functional recovery. As such, there is a clinical need for an off-the-shelf, bioactive scaffold that directs patient’s cells to align and differentiate into muscle tissue in situ. In this thesis, we developed fibrin microthreads, scaffolds composed of aligned fibrin material that direct cell alignment along the longitudinal axis of the microthread structure, with specific structural and biochemical properties to recreate structural cues lost in VML injuries. We hypothesized that fibrin microthreads with an increased resistance to proteolytic degradation and loaded with HGF would enhance the functional, mechanical regeneration of skeletal muscle tissue in a VML injury. We developed a crosslinking strategy to increase fibrin microthread resistance to enzymatic degradation, and increased their tensile strength and stiffness two- to three-fold. This crosslinking strategy enhanced the adsorption of HGF, facilitated its rapid release from microthreads for 2 to 3 days, and increased the chemotactic response of myoblasts twofold in 2D and 3D assays. Finally, we implanted HGF-loaded, crosslinked (EDCn-HGF) microthreads into a mouse model of VML to evaluate tissue regeneration and functional recovery. Fourteen days post-injury, we observed more muscle ingrowth along EDCn-HGF microthreads than untreated controls, suggesting that released HGF recruited additional progenitor cells to the injury site. Sixty days post-injury, EDCn-HGF microthreads guided mature, organized muscle to replace the microthreads in the wound site. Further, EDCn-HGF microthreads restored the contractile mechanical strength of the tissue to pre-injured values. In summary, we designed fibrin microthreads that recapitulate regenerative cues lost in VML injuries and enhance the functional regeneration of skeletal muscle.
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Ravi, Swathi. "Recombinant elastin analogues as cell-adhesive matrices for vascular tissue engineering." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/42728.
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Biomimetic materials that recapitulate the complex mechanical and biochemical cues in load-bearing tissues are of significant interest in regenerative medicine and tissue engineering applications. Several investigators have endeavored to not only emulate the mechanical properties of the vasculature, but to also mimic the biologic responsiveness of the blood vessel in creating vascular substitutes. Previous studies in our lab generated the elastin-like protein polymer LysB10, which was designed with the capability of physical and chemical crosslinks, and was shown to display a range of elastomeric properties that more closely matched those of the native artery. While extensive validation of the mechanical properties of elastin-mimetic polymers has demonstrated their functionality in a number of tissue engineering applications, limited cell growth on the surfaces of the polymers has motivated further optimization for biological interaction. Recent biologically-inspired surface strategies have focused on functionalizing material surfaces with extracellular matrix molecules and bioactive motifs in order to encourage integrin-mediated cellular responses that trigger precise intracellular signaling processes, while limiting nonspecific biomaterial interactions. Consequently, this dissertation addresses three approaches to modulating cellular behavior on elastin-mimetic analogs with the goal of promoting vascular wall healing and tissue regeneration: genetic engineering of elastin-like protein polymers (ELPs) with cell-binding domains, biofunctionalization of elastin-like protein polymers via chemoselective ligation of bioactive ligands, and incorporation of matrix protein fibronectin for engineering of cell-seeded multilamellar collagen-reinforced elastin-like constructs.
The synthesis of recombinant elastin-like protein polymers that integrate biologic functions of the extracellular matrix provides a novel design strategy for generating clinically durable vascular substitutes. Ultimately, the synthesis of model protein networks provides new insights into the relationship between molecular architecture, biomimetic ligand presentation, and associated cellular responses at the cell-material interface. Understanding how each of these design parameters affects cell response will contribute significantly to the rational engineering of bioactive materials. Potential applications for polymer blends with enhanced mechanical and biological properties include surface coatings on vascular grafts and stents, as well as composite materials for tissue engineered scaffolds and vascular substitutes.
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Mhuka, Vimbai. "Characterization of silk proteins from African wild silkworm cocoons and application of fibroin matrices as biomaterials." Thesis, 2014. http://hdl.handle.net/10500/19145.
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Challenges in treating injuries, together with an increased need for repair of damaged tissues and organs, have made regenerative medicine a major research area today. Biomaterials such as silk fibroin (SF) have proven to be excellent tissue scaffolds possessing properties essential in tissue engineering such as biocompatibility, biodegradability and exceptional mechanical properties. SF nanofibres are especially attractive due to their large surface-to-volume ratio and high porosity which is beneficial in regenerative medicine. However, to design biomaterial scaffolds, chemical and physical properties of SF have to be sufficiently known. The thesis aims to contribute to knowledge by characterizing silk fibroin from the African wild silkworm species Gonometa rufobrunnae, Gonometa postica, Argema mimosae, Epiphora bahuniae and Anaphe panda. Moreover, the feasibility of producing nanofibrous biomaterial scaffolds from these fibroins is explored.
The chemical composition of degummed fibres was investigated using Capillary electrophoresis whilst Infrared (IR) and Raman spectroscopic techniques were utilized to determine structural characteristics of the fibroin. In addition, thermal behaviour and mechanical properties of the fibroins were also investigated. Nanofibres were fabricated via electrospinning. The effects of solution concentration, voltage, polymer flow rate and tip to collector distance were studied to give optimum electrospinning conditions. IR spectroscopy was also utilized to observe the conformational structure of the degummed and electrospun fibres whilst scanning electron microscopy (SEM) provided information on the size and morphology of the fibres. The use of the nanofibres as biomaterials was evaluated using cytotoxicity tests.
Results showed that glycine, alanine and serine constituted over 70% of the amino acid composition of all the fibroins. Gonometa fibroin had more glycine than alanine whilst the opposite was true for Argema mimosae, Epiphora bahuniae and Anaphe panda fibroin. The abundance of basic amino acids in Gonometa rufobrunnae, Gonometa postica, Argema mimosae and Epiphora bahuniae fibroin makes them prime candidates for cell and tissue culture. The amino acid composition of the fibroins influenced secondary structure as the β-sheet structure. Anaphe panda, Argema mimosae and Epiphora bahuniae silks was made up of mostly alanine-alanine (Ala-Ala)n polypeptides whilst Gonometa fibroin had an interesting mixture of both glycine-alanine (Gly-Ala)n and (Ala-Ala)n units. The unique structures impacted the mechanical and thermal properties of the fibroins.
Production of Gonometa nanofibres was mainly dependent on fibroin solution concentration. A minimum of 27 % w/v was needed to produce defect free nanofibres. Diameters of the electrospun fibres produced ranged from 300 to 2500 nm. IR spectroscopy data highlighted that the β-sheet conformation of degummed fibroin was degraded during the formation of the nanofibres rendering them water soluble. It was however possible to regenerate the β-sheet structure in the nanofibres by exposing them to various solvents. Cytotoxicity tests using Sulforhodamine B (SRB) assay demonstrated that the nanofibres were not toxic to cells, a major prerequisite for use as a biomaterial.
This thesis successfully provides useful data in an area that has been minimally explored. Results suggest that SF from African silkworm species offers diversity in properties and are therefore attractive for use as biomaterials, especially in cell and tissue engineering. As far as we could determine, we are the first to extend the use of fibroin from African silk species by producing Gonometa SF nanofibres that are of potential use as biomaterial scaffolds.Chemistry
D. Phil. (Chemisty)
Book chapters on the topic "Biomaterials, regenerative medicine, carbohydrates, proteins"
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Olgierd, Batoryna, Aleksandra Sklarek, Paulina Siwek, and Ewa Waluga. "Methods of Biomaterial-Aided Cell or Drug Delivery: Extracellular Matrix Proteins as Biomaterials." In Stem Cells and Biomaterials for Regenerative Medicine, 163–89. Elsevier, 2019. http://dx.doi.org/10.1016/b978-0-12-812258-7.00011-3.
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Banerjee, J., E. Radvar, and H. S. Azevedo. "Self-assembling peptides and their application in tissue engineering and regenerative medicine." In Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair, 245–81. Elsevier, 2018. http://dx.doi.org/10.1016/b978-0-08-100803-4.00010-3.
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