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Journal articles on the topic 'Bioluminescent detection'

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Author: Grafiati

Published: 10 December 2022

Last updated: 28 January 2023

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1

Dostálek, P., and T. Brányik. "Prospects for rapid bioluminescent detection methods in the food industry – a review." Czech Journal of Food Sciences 23, No. 3 (November 15, 2011): 85–92. http://dx.doi.org/10.17221/3376-cjfs.

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This review surveys rapid bioluminescent detection techniques applied in food industry and discusses the historical development of the rapid methods. These techniques are divided into two groups: methods based on bioluminescent adenosine triphosphate (ATP) assay, and on bacterial bioluminescence. The advantages and disadvantages of these methods are described. The article provides the bibliography of fluorescent method applications in food samples.    

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2

Wang, Ke-Yong, Chun Wu, Shohei Shimajiri, Toshiteru Enomoto, Hidehiro Kubota, Hidefumi Akiyama, and Yoshihiro Ohmiya. "Quantitative immunohistochemistry using an antibody-fused bioluminescent protein." BioTechniques 69, no. 4 (October 2020): 302–6. http://dx.doi.org/10.2144/btn-2020-0006.

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We established a quantitative detection method for immunohistochemistry based on a reference standard light-emitting diode, protein microarray and antibody-fused bioluminescent protein. In this procedure, we calibrated the bioluminescence imaging system and prepared the calibration curve between antigen and antibody-fused bioluminescent protein using a protein microarray. Then we converted the detecting light signal to antigen count via absolute photon number in the bioluminescent images; there was a resulting threefold difference in the target antigen number between normal and cancerous tissues. Our technique can easily compare immunohistological images and evaluate tumor progression in quantitative pathological diagnosis.

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3

Goueli, Said A., Kevin Hsiao, and Hicham Zegzouti. "Bioluminescent AMP Detection." Genetic Engineering & Biotechnology News 33, no. 15 (September 2013): 24. http://dx.doi.org/10.1089/gen.33.15.10.

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4

Zajíc, Jakub, Steven Ripp, Josef Trögl, Gabriela Kuncová, and Marie Pospíšilová. "Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements." Sensors 20, no. 11 (June 6, 2020): 3237. http://dx.doi.org/10.3390/s20113237.

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In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with P. putida TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced P. putida TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg/L was detected after <30 min after immersion of the probe in the toluene solution. Additional experiments also immobilized constitutively bioluminescent cells of E. coli 652T7, on OFEs with polyethyleneimine (PEI). These OFEs were repetitively induced with Lauria-Bertani (LB) nutrient medium. Bioluminescence appeared 15 minutes after immersion of the OFE in LB. A change in pH from 7 to 6 resulted in a decrease in bioluminescence that was not restored following additional nutrient inductions at pH 7. The E. coli 652T7 OFE probe was therefore sensitive to negative influences but could not be repetitively used.

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5

Layton, A. C., M. Muccini, M. M. Ghosh, and G. S. Sayler. "Construction of a Bioluminescent Reporter Strain To Detect Polychlorinated Biphenyls." Applied and Environmental Microbiology 64, no. 12 (December 1, 1998): 5023–26. http://dx.doi.org/10.1128/aem.64.12.5023-5026.1998.

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ABSTRACT A bioluminescent reporter strain, Ralstonia eutrophaENV307(pUTK60), was constructed for the detection of polychlorinated biphenyls by inserting the biphenyl promoter upstream of the bioluminescence genes. In the presence of a nonionic surfactant, which enhances the solubility of chlorinated biphenyls, bioluminescence was induced three- to fourfold over background by biphenyl, monochlorinated biphenyls, and Aroclor 1242. The minimum detection limits for these compounds ranged from 0.15 mg/liter for 4-chlorobiphenyl to 1.5 mg/liter for Aroclor 1242.

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6

Hay, Anthony G., James F. Rice, Bruce M. Applegate, Nathan G. Bright, and Gary S. Sayler. "A Bioluminescent Whole-Cell Reporter for Detection of 2,4-Dichlorophenoxyacetic Acid and 2,4-Dichlorophenol in Soil." Applied and Environmental Microbiology 66, no. 10 (October 1, 2000): 4589–94. http://dx.doi.org/10.1128/aem.66.10.4589-4594.2000.

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ABSTRACT A bioreporter was made containing atfdRPDII-luxCDABE fusion in a modified mini-Tn5 construct. When it was introduced into the chromosome of Ralstonia eutropha JMP134, the resulting strain, JMP134-32, produced a sensitive bioluminescent response to 2,4-dichlorophenoxyacetic acid (2,4-D) at concentrations of 2.0 μM to 5.0 mM. This response was linear (R 2 = 0.9825) in the range of 2.0 μM to 1.1 × 102 μM. Saturation occurred at higher concentrations, with maximal bioluminescence occurring in the presence of approximately 1.2 mM 2,4-D. A sensitive response was also recorded in the presence of 2,4-dichlorophenol at concentrations below 1.1 × 102μM; however, only a limited bioluminescent response was recorded in the presence of 3-chlorobenzoic acid at concentrations below 1.0 mM. A significant bioluminescent response was also recorded when strain JMP134-32 was incubated with soils containing aged 2,4-D residues.

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7

Simonyan, Hayk, Chansol Hurr, and Colin N. Young. "A synthetic luciferin improves in vivo bioluminescence imaging of gene expression in cardiovascular brain regions." Physiological Genomics 48, no. 10 (October 1, 2016): 762–70. http://dx.doi.org/10.1152/physiolgenomics.00055.2016.

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Bioluminescence imaging is an effective tool for in vivo investigation of molecular processes. We have demonstrated the applicability of bioluminescence imaging to spatiotemporally monitor gene expression in cardioregulatory brain nuclei during the development of cardiovascular disease, via incorporation of firefly luciferase into living animals, combined with exogenous d-luciferin substrate administration. Nevertheless, d-luciferin uptake into the brain tissue is low, which decreases the sensitivity of bioluminescence detection, particularly when considering small changes in gene expression in tiny central areas. Here, we tested the hypothesis that a synthetic luciferin, cyclic alkylaminoluciferin (CycLuc1), would be superior to d-luciferin for in vivo bioluminescence imaging in cardiovascular brain regions. Male C57B1/6 mice underwent targeted delivery of an adenovirus encoding the luciferase gene downstream of the CMV promoter to the subfornical organ (SFO) or paraventricular nucleus of hypothalamus (PVN), two crucial cardioregulatory neural regions. While bioluminescent signals could be obtained following d-luciferin injection (150 mg/kg), CycLuc1 administration resulted in a three- to fourfold greater bioluminescent emission from the SFO and PVN, at 10- to 20-fold lower substrate concentrations (7.5–15 mg/kg). This CycLuc1-mediated enhancement in bioluminescent emission was evident early following substrate administration (i.e., 6–10 min) and persisted for up to 1 h. When the exposure time was reduced from 60 s to 1,500 ms, minimal signal in the PVN was detectable with d-luciferin, whereas bioluminescent images could be reliably captured with CycLuc1. These findings demonstrate that bioluminescent imaging with the synthetic luciferin CycLuc1 provides an improved physiological genomics tool to investigate molecular events in discrete cardioregulatory brain nuclei.

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8

Wang, W., M. Zhang, J. Fang, L. Zhang, X. Zou, and X. Wang. " Improved detection of Ochratoxin A by marine bioluminescent bacteria V. harveyi BA." Czech Journal of Food Sciences 31, No. 1 (January 10, 2013): 88–93. http://dx.doi.org/10.17221/18/2012-cjfs.

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We applicate the bioluminescent assay system for evaluating the toxicity of Ochratoxin A (OTA). The optimum conditions for the growth and bioluminescence of V. harveyi BA were investigated, including NaCl concentration and pH in the medium, incubation temperature, and OTA action time. The growth and luminescence reached the perfect phase with the NaCl concentration in the range of 1% to 2%, pH 8–9, incubation temperature 25–30°C, and OTA acting for1 hour. Based on these optimum conditions for bioluminescence, the inhibitory effect of OTA on luminosity was pursued. When OTA concentration fell into the range of 0.1–1.0 µg/l, bioluminescence inhibition followed a linear pattern with a good correlation coefficient (R<sup>2</sup> = 0.944). The calculated recovery percentages fell into the range of 81–102% within the spiking range of 20–200 µg/kg. This system provided a screening method for the measurement of toxic OTA by monitoring the changes in luminescence.  

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9

Jung, Insup, Ho Bin Seo, Ji-eun Lee, Byoung Chan Kim, and Man Bock Gu. "A dip-stick type biosensor using bioluminescent bacteria encapsulated in color-coded alginate microbeads for detection of water toxicity." Analyst 139, no. 18 (2014): 4696–701. http://dx.doi.org/10.1039/c4an00308j.

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The use of genetically engineered bioluminescent bacteria, in which bioluminescence is induced by different modes of toxic action, represents an alternative to acute toxicity tests using living aquatic organisms (plants, vertebrates, or invertebrates) in an aqueous environment.

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10

Elena, Ionescu Rodica, Jia Kun, Eltzov Evgeni, and Marks Robert. "Improved bioluminescent detection of pesticides." Current Opinion in Biotechnology 22 (September 2011): S37. http://dx.doi.org/10.1016/j.copbio.2011.05.086.

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11

Hersh, Jessica, Yu-Ping Yang, Evan Roberts, Daniel Bilbao, Wensi Tao, Sylvia Daunert, and Sapna Deo. "Abstract 2471: Targeted dendrimer nanocarrier delivery of bioluminescent proteins for pancreatic cancer detection." Cancer Research 82, no. 12_Supplement (June 15, 2022): 2471. http://dx.doi.org/10.1158/1538-7445.am2022-2471.

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Abstract Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer and has a very low survival rate. This cancer often does not show symptoms until more advanced stages, leading to late-stage diagnosis in up to 80% of cases. Molecular tools for accurate identification of the cancer cells can lead to a timely diagnosis. Currently, blood antigen testing with CA 19-9 and tumor imaging using ultrasound, CT, and MRI are the standards for identification. However, these methods are limited in their ability to discern key attributes such as tumor localization, grading, and metastasis. To overcome this, we can utilize selective recognition of PDAC markers to deliver a bioluminescent protein for targeted imaging. We applied dendrimers as a nanocarrier that can hold both the PDAC-targeting protein and the bioluminescent protein for enhancement of blood-circulation and tumor-retention time, thereby producing a stronger, longer lasting luminescent signal. This cell-specific recognition assembly can locate both localized and metastatic PDAC cells, enabling an accurate diagnosis, localization of tumor margin, and identification of metastases using bioluminescence-based imaging. The targeted construct was formed by fusing an EGFR-specific affibody to Gaussia luciferase, a bioluminescent protein, and binding that to a G5 polyamidoamine dendrimer nanocarrier. Western Blotting and confocal microscopy using PANC 10.05 cells confirmed the expression of EGFR and enhanced uptake of the targeted construct in vitro. For in vivo studies, female NSG mice were used as a xenograft model with PANC 10.05 cells with either subcutaneous, renal capsule, or orthotopic tumors. Tumor-bearing mice were treated with dendrimer-fusion protein constructs and imaged using IVIS. Controls included non-targeted bioluminescent proteins and non-tumor-bearing mice. The presence of EGFR on the tumors of implanted mice was confirmed using Western blotting and histological staining. We showed that the EGFR-targeted construct was capable of facilitating selective uptake into the tumors in all three mouse models, demonstrating that the cell-specific construct could localize to the PDAC cells and emit bioluminescence when imaged with IVIS. Accuracy of the tumor localization was also confirmed with ultrasound. Additionally, we demonstrated the ability of the construct to locate metastases using both live-animal imaging and ex vivo organ analysis. These bioluminescent nanocarriers are capable of PDAC cell-specific recognition and can be used to locate and image PDAC cells. This methodology can then be applied to mice without confirmed tumors to demonstrate the ability to achieve timely diagnosis for early detection of PDAC. Ultimately, this construct can aid in the identification and localization of PDAC. Citation Format: Jessica Hersh, Yu-Ping Yang, Evan Roberts, Daniel Bilbao, Wensi Tao, Sylvia Daunert, Sapna Deo. Targeted dendrimer nanocarrier delivery of bioluminescent proteins for pancreatic cancer detection [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 2471.

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12

Francis, Kevin P., Danny Joh, Carolyn Bellinger-Kawahara, Matthew J. Hawkinson, Tony F. Purchio, and Pamela R. Contag. "Monitoring Bioluminescent Staphylococcus aureusInfections in Living Mice Using a Novel luxABCDEConstruct." Infection and Immunity 68, no. 6 (June 1, 2000): 3594–600. http://dx.doi.org/10.1128/iai.68.6.3594-3600.2000.

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ABSTRACT Strains of Staphylococcus aureus were transformed with plasmid DNA containing a Photorhabdus luminescens luxoperon (luxABCDE) that was genetically modified to be functional in both gram-positive and gram-negative bacteria. S. aureus cells containing this novel lux construct, downstream of an appropriate promoter sequence, are highly bioluminescent, allowing the detection of fewer than 100 CFU in vitro (direct detection of exponentially dividing cells in liquid culture). Furthermore, these bacteria produce light stably at 37°C and do not require exogenous aldehyde substrate, thus allowing S. aureus infections in living animals to be monitored by bioluminescence. Two strains of S. aureus 8325-4 that produce high levels of constitutive bioluminescence were injected into the thigh muscles of mice, and the animals were then either treated with the antibiotic amoxicillin or left untreated. Bioluminescence from bacteria present in the thighs of the mice was monitored in vivo over a period of 24 h. The effectiveness of the antibiotic in the treated animals could be measured by a decrease in the light signal. At 8 h, the infection in both groups of treated animals had begun to clear, as judged by a decrease in bioluminescence, and by 24 h no light signal could be detected. In contrast, both groups of untreated mice had strong bioluminescent signals at 24 h. Quantification of CFU from bacteria extracted from the thigh muscles of the mice correlated well with the bioluminescence data. This paper shows for the first time that bioluminescence offers a method for monitoring S. aureus infections in vivo that is sensitive and noninvasive and requires fewer animals than conventional methodologies.

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13

Shao, C. Y., C. J. Howe, A. J. R. Porter, and L. A. Glover. "Novel Cyanobacterial Biosensor for Detection of Herbicides." Applied and Environmental Microbiology 68, no. 10 (October 2002): 5026–33. http://dx.doi.org/10.1128/aem.68.10.5026-5033.2002.

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ABSTRACT The aim of this work was to generate a cyanobacterial biosensor that could be used to detect herbicides and other environmental pollutants. A representative freshwater cyanobacterium, Synechocystis sp. strain PCC6803, was chromosomally marked with the luciferase gene luc (from the firefly Photinus pyralis) to create a novel bioluminescent cyanobacterial strain. Successful expression of the luc gene during growth of Synechocystis sp. strain PCC6803 cultures was characterized by measuring optical density and bioluminescence. Bioluminescence was optimized with regard to uptake of the luciferase substrate, luciferin, and the physiology of the cyanobacterium. Bioassays demonstrated that a novel luminescent cyanobacterial biosensor has been developed which responded to a range of compounds including different herbicide types and other toxins. This biosensor is expected to provide new opportunities for the rapid screening of environmental samples or for the investigation of potential environmental damage.

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14

Ravindran, J., B. Manikandan, P. V. Shirodkar, K. X. Francis, R. Mani Murali, and P. Vethamony. "Bacterial bioluminescence response to long-term exposure to reverse osmosis treated effluents from dye industries." Canadian Journal of Microbiology 60, no. 10 (October 2014): 661–68. http://dx.doi.org/10.1139/cjm-2014-0378.

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The bacterial bioluminescence assay is one of the novel means for toxicity detection. The bioluminescence response of 2 marine bioluminescent bacteria was tested upon their long-term exposure to 9 different reverse osmosis (RO) rejects with varying chemical composition sampled from various dye industries. Bioluminescent bacteria were cultured in the RO reject samples, at different concentrations, and their growth rate and luminescence was measured for 24 h. The RO reject samples caused sublethal effects upon exposure and retarded the growth of bacteria, confirming their toxic nature. Further, continuation of the exposure showed that the initial luminescence, though reduced, recovered and increased beyond the control cultures irrespective of cell density, and finally decreased once again. The present study emphasizes the need of evolving a long-term exposure assay and shows that the method followed in this study is suitable to evaluate the toxicants that exert delayed toxicity, using lower concentrations of toxicants as well as coloured samples.

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15

BAKER, J. M., M. W. GRIFFITHS, and D. L. COLLINS-THOMPSON. "Bacterial Bioluminescence: Applications in Food Microbiology." Journal of Food Protection 55, no. 1 (January 1, 1992): 62–70. http://dx.doi.org/10.4315/0362-028x-55.1.62.

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Many marine microorganisms (Vibrio, Photobacterium) are capable of emitting light, that is, they are bioluminescent. The light-yielding reaction is catalyzed by a luciferase, and it involves the oxidation of reduced riboflavin phosphate and a long-chain aldehyde in the presence of oxygen to produce a blue green light. The genes responsible for the luciferase production, (lux A and lux B), aldehyde synthesis (lux C, D, and E), and regulation of luminescence (lux I and lux R) have all been identified, and recent research has resulted in the discovery of three new genes (lux F, G, and H). The ability to genetically engineer dark microorganisms to become light emitting by introducing the lux genes into them has opened up a wide range of applications of bioluminescence. Assays using bacterial bioluminescence for the detection and enumeration of microorganisms are rapid, sensitive, accurate, and can be made specific. It is these attributes that are making in vivo bioluminescent assays so attractive to the food industry.

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Frundzhyan, Valery G., Inna M. Parkhomenko, Lubov Y. Brovko, and Natalia N. Ugarova. "Improved bioluminescent assay of somatic cell counts in raw milk." Journal of Dairy Research 75, no. 3 (June 2, 2008): 279–83. http://dx.doi.org/10.1017/s0022029908003282.

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Somatic cell count (SCC) in milk is considered to be a valuable indicator of cow mastitis. For assessment of SCC in milk, the bioluminescent assay based on determination of ATP from somatic cells ([ATPsom]) in milk was proposed earlier. However, this assay is still not widely used in practice owing to lower reliability compared with conventional methods such as direct microscopy and flow cytometry. We revised the bioluminescent SCC assay and developed a simple protocol based on determination of the total non-bacterial ATP concentration in milk. It was shown that the novel ATP-releasing agent Neonol-10 (oxy-ethylated iso-nonyl phenol) has superior performance providing 100% lysis of somatic cells while not disrupting bacterial cells of milk at a concentration of 1·5% w/w. There was high correlation (R2=0·99) between measured bioluminescence and SCC as measured by direct microscopy. The observed detection limit of the bioluminescent milk SCC assay was as low as 900 cell/ml, time of analysis was 2–3 min per sample. The proposed method has high potential for on-site mastitis diagnostics.

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17

Schofield, D. A., and C. Westwater. "Phage-mediated bioluminescent detection ofBacillus anthracis." Journal of Applied Microbiology 107, no. 5 (November 2009): 1468–78. http://dx.doi.org/10.1111/j.1365-2672.2009.04332.x.

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18

Gaspar, Natasa, Giorgia Zambito, Iris J. C. Dautzenberg, Steve J. Cramer, Rob C. Hoeben, Clemens Lowik, Joel R. Walker, et al. "NanoBiT System and Hydrofurimazine for Optimized Detection of Viral Infection in Mice—A Novel in Vivo Imaging Platform." International Journal of Molecular Sciences 21, no. 16 (August 15, 2020): 5863. http://dx.doi.org/10.3390/ijms21165863.

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Reporter genes are used to visualize intracellular biological phenomena, including viral infection. Here we demonstrate bioluminescent imaging of viral infection using the NanoBiT system in combination with intraperitoneal injection of a furimazine analogue, hydrofurimazine. This recently developed substrate has enhanced aqueous solubility allowing delivery of higher doses for in vivo imaging. The small high-affinity peptide tag (HiBiT), which is only 11 amino-acids in length, was engineered into a clinically used oncolytic adenovirus, and the complementary large protein (LgBiT) was constitutively expressed in tumor cells. Infection of the LgBiT expressing cells with the HiBiT oncolytic virus will reconstitute NanoLuc in the cytosol of the cell, providing strong bioluminescence upon treatment with substrate. This new bioluminescent system served as an early stage quantitative viral transduction reporter in vitro and also in vivo in mice, for longitudinal monitoring of oncolytic viral persistence in infected tumor cells. This platform provides novel opportunities for studying the biology of viruses in animal models.

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Briestenská, Katarína, Miriam Mikušová, Karolína Tomčíková, František Kostolanský, and Eva Varečková. "Quantification of bacteria by in vivo bioluminescence imaging in comparison with standard spread plate method and reverse transcription quantitative PCR (RT-qPCR)." Archives of Microbiology 203, no. 7 (June 29, 2021): 4737–42. http://dx.doi.org/10.1007/s00203-021-02458-5.

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AbstractIn vivo bioluminescence imaging (BLI) offers a unique opportunity to analyze ongoing bacterial infections qualitatively and quantitatively in intact animals over time, leading to a reduction in the number of animals needed for a study. Since accurate determination of the bacterial burden plays an essential role in microbiological research, the present study aimed to evaluate the ability to quantify bacteria by non-invasive BLI technique in comparison to standard spread plate method and reverse transcription quantitative PCR (RT-qPCR). For this purpose, BALB/c mice were intranasally infected with 1 × 105 CFU of bioluminescent Streptococcus pneumoniae A66.1. At day 1 post-infection, the presence of S. pneumoniae in lungs was demonstrated by spread plate method and RT-qPCR, but not by in vivo BLI. However, on the second day p.i., the bioluminescent signal was already detectable, and the photon flux values positively correlated with CFU counts and RT-qPCR data within days 2–6. Though in vivo BLI is valuable research tool allowing the continuous monitoring and quantification of pneumococcal infection in living mice, it should be kept in mind that early in the infection, depending on the infective dose, the bioluminescent signal may be below the detection limit.

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20

Kricka, L. J. "Chemiluminescent and bioluminescent techniques." Clinical Chemistry 37, no. 9 (September 1, 1991): 1472–81. http://dx.doi.org/10.1093/clinchem/37.9.1472.

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Abstract Light-emitting chemical reactions (chemiluminescence, CL) and biological reactions (bioluminescence, BL) have a diverse range of analytical applications but relatively few have been adopted by routine clinical laboratories. Advantages of CL and BL assays include sensitivity (attomole and sub-attomole detection limits), speed (signal generated in a few seconds and in some cases stable for several hours), nonhazardous reagents, and simple procedures. The most promising clinical applications are in immunoassay, protein blotting, and DNA probe assays. Chemiluminescent molecules exploited as labels include luminol, isoluminol, acridinium esters, thioesters and sulfonamides, and phenanthridinium esters. Separation and nonseparation assays have been devised, based on isoluminol and acridinium ester labels. The combination of the amplification properties of an enzyme and a CL or BL detection reaction provides a highly sensitive analytical system. Since 1983, CL and BL methods have been developed for many enzyme labels, e.g., alkaline phosphatase, glucose-6-phosphate dehydrogenase, horseradish peroxidase, Renilla luciferase, and xanthine oxidase. Currently, the most successful enzyme assays are the enhanced CL method for a peroxidase label involving a mixture of luminol, hydrogen peroxide, and an enhancer (e.g., p-iodophenol) and the direct CL method for alkaline phosphatase, with an adamantyl 1,2-dioxetane phenyl phosphate as substrate. Both systems are very sensitive (the detection limit for alkaline phosphatase when using the dioxetane reagent is 0.001 amol) and produce long-lived light emission (greater than 30 min), which is ideal for membrane applications in which light emission is detected with photographic film or a charge-coupled device camera.

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Wiles, Siouxsie, Karen M. Pickard, Katian Peng, Thomas T. MacDonald, and Gad Frankel. "In Vivo Bioluminescence Imaging of the Murine Pathogen Citrobacter rodentium." Infection and Immunity 74, no. 9 (September 2006): 5391–96. http://dx.doi.org/10.1128/iai.00848-06.

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ABSTRACT Citrobacter rodentium is a natural mouse pathogen related to enteropathogenic and enterohemorrhagic Escherichia coli. We have previously utilized bioluminescence imaging (BLI) to determine the in vivo colonization dynamics of C. rodentium. However, due to the oxygen requirement of the bioluminescence system and the colonic localization of C. rodentium, in vivo localization studies were performed using harvested organs. Here, we report the detection of bioluminescent C. rodentium and commensal E. coli during colonization of the gastrointestinal tract in intact living animals. Bioluminescence was dependent on intact blood circulation, suggesting that the colonic environment is not anaerobic but nanaerobic. In addition, BLI revealed that C. rodentium colonizes the rectum, a site previously unreported for this pathogen.

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22

Dane, Fenny. "USE OF BIOLUMINESCENCE FOR THE DETECTION OF THE EFFECTIVENESS OF BIOLOGICAL CONTROL AGENTS ON BLACK ROT DISEASE OF CABBAGE." HortScience 31, no. 5 (September 1996): 760f—760. http://dx.doi.org/10.21273/hortsci.31.5.760f.

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A genetically engineered, bioluminescent strain of Xanthomonas campestris pv. campestris (Xcc) was used to study the effectiveness of plant growth-promoting rhizobacteria (PGPR) as disease control agents. Black-rot-susceptible cabbage plants were wound-inoculated with PGPR and wound- or mist-inoculated with bioluminescent Xcc 10 days later. Growth of the bioluminescent strain in the plants was followed over time with a low-light, charge-coupled device camera. Several PGPR strains effectively reduced growth of the bioluminescent pathogen in the plants when bacteria were introduced into the plant by wound. PGPR inoculation was less effective when bioluminescent bacteria were introduced into the plant by mist inoculation. Little effect on symptom reduction was observed.

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23

Xuan, Guanhua, Qilin Xiao, Jingxue Wang, and Hong Lin. "Cloning and expression of the flavin reductase LuxG from Photobacterium leiognathi YL and its improvement for NADH detection." Photochemical & Photobiological Sciences 19, no. 2 (2020): 274–80. http://dx.doi.org/10.1039/c9pp00435a.

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LuxG from P. leiognathi YL was successfully purified and characterized. Based on this, a coupled pure enzyme bioluminescent system was established and used for NADH detection, which showed higher sensitivity than existing bioluminescent systems.

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Sanseverino, John, Rakesh K. Gupta, Alice C. Layton, Stacey S. Patterson, Steven A. Ripp, Leslie Saidak, Michael L. Simpson, T. Wayne Schultz, and Gary S. Sayler. "Use of Saccharomyces cerevisiae BLYES Expressing Bacterial Bioluminescence for Rapid, Sensitive Detection of Estrogenic Compounds." Applied and Environmental Microbiology 71, no. 8 (August 2005): 4455–60. http://dx.doi.org/10.1128/aem.71.8.4455-4460.2005.

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ABSTRACT An estrogen-inducible bacterial lux-based bioluminescent reporter was developed in Saccharomyces cerevisiae for applications in chemical sensing and environmental assessment of estrogen disruptor activity. The strain, designated S. cerevisiae BLYES, was constructed by inserting tandem estrogen response elements between divergent yeast promoters GPD and ADH1 on pUTK401 (formerly pUA12B7) that constitutively express luxA and luxB to create pUTK407. Cotransformation of this plasmid with a second plasmid (pUTK404) containing the genes required for aldehyde synthesis (luxCDE) and FMN reduction (frp) yielded a bioluminescent bioreporter responsive to estrogen-disrupting compounds. For validation purposes, results with strain BLYES were compared to the colorimetric-based estrogenic assay that uses the yeast lacZ reporter strain (YES). Strains BLYES and YES were exposed to 17β-estradiol over the concentration range of 1.2 × 10−8 through 5.6 × 10−12 M. Calculated 50% effective concentration values from the colorimetric and bioluminescence assays (n = 7) were similar at (4.4 ± 1.1) × 10−10 and (2.4 ± 1.0) × 10−10 M, respectively. The lower and upper limits of detection for each assay were also similar and were approximately 4.5 × 10−11 to 2.8 × 10−9 M. Bioluminescence was observed in as little as 1 h and reached its maximum in 6 h. In comparison, the YES assay required a minimum of 3 days for results. Strain BLYES fills the niche for rapid, high-throughput screening of estrogenic compounds and has the ability to be used for remote, near-real-time monitoring of estrogen-disrupting chemicals in the environment.

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MURAMATSU, Tatsuo, Kazuteru FUKAZAWA, and Akihiro NAKAMURA. "Live Detection of Bioluminescent Preimplantation Bovine Embryos." Nihon Chikusan Gakkaiho 70, no. 6 (1999): 437–43. http://dx.doi.org/10.2508/chikusan.70.437.

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Prante, Marc, Christian Ude, Miriam Große, Lukas Raddatz, Ulrich Krings, Gernot John, Shimshon Belkin, and Thomas Scheper. "A Portable Biosensor for 2,4-Dinitrotoluene Vapors." Sensors 18, no. 12 (December 3, 2018): 4247. http://dx.doi.org/10.3390/s18124247.

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Buried explosive material, e.g., landmines, represent a severe issue for human safety all over the world. Most explosives consist of environmentally hazardous chemicals like 2,4,6-trinitrotoluene (TNT), carcinogenic 2,4-dinitrotoluene (2,4-DNT) and related compounds. Vapors leaking from buried landmines offer a detection marker for landmines, presenting an option to detect landmines without relying on metal detection. 2,4-Dinitrotoluene (DNT), an impurity and byproduct of common TNT synthesis, is a feasible detection marker since it is extremely volatile. We report on the construction of a wireless, handy and cost effective 2,4-dinitrotoluene biosensor combining recombinant bioluminescent bacterial cells and a compact, portable optical detection device. This biosensor could serve as a potential alternative to the current detection technique. The influence of temperature, oxygen and different immobilization procedures on bioluminescence were tested. Oxygen penetration depth in agarose gels was investigated, and showed that aeration with molecular oxygen is necessary to maintain bioluminescence activity at higher cell densities. Bioluminescence was low even at high cell densities and 2,4-DNT concentrations, hence optimization of different prototypes was carried out regarding radiation surface of the gels used for immobilization. These findings were applied to sensor construction, and 50 ppb gaseous 2,4-DNT was successfully detected.

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Yakimov, Anton S., Ivan A. Denisov, Anton S. Bukatin, Kirill A. Lukyanenko, Kirill I. Belousov, Igor V. Kukhtevich, Elena N. Esimbekova, Anatoly A. Evstrapov, and Peter I. Belobrov. "Droplet Reactors with Bioluminescent Enzymes for Real-Time Water Pollution Monitoring." Proceedings 60, no. 1 (November 25, 2020): 54. http://dx.doi.org/10.3390/iecb2020-07046.

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Early detection of pollutants in wastewater, water coming out of treatment facilities, drinking water, and water for agricultural needs is a challenging problem. Effective water quality monitoring requires development of new methods for express detection of pollutants. Enzymes from bioluminescent bacteria can be used for the development of new express enzyme-based bioassay systems. This work demonstrates, for the first time, a microfluidic chip to generate emulsion droplets containing two enzymes of the bacterial bioluminescent system (luciferase and NAD(P)H:FMN-oxidoreductase) with reaction substrates. The developed chip generated “water-in-oil” emulsion droplets with a volume of 0.1 μL and a frequency of up to 12 droplets per second. A portable photomultiplier tube (PMT) was used to measure the bioluminescent signal in each individual droplet; the signal-to-noise ratio was 3000/1. The intensity of luminescence in droplets depended on the concentration of copper ions. The limit of detection (LOD) for copper sulfate was 1 mg/L. We showed that bioluminescent enzymatic reactions can be carried out in droplet reactors that can be applied for online monitoring of water quality. Thus, the suggested method of biological measurements has a good perspective for biosensing in general.

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Eldridge, Melanie L., John Sanseverino, Alice C. Layton, James P. Easter, T. Wayne Schultz, and Gary S. Sayler. "Saccharomyces cerevisiae BLYAS, a New Bioluminescent Bioreporter for Detection of Androgenic Compounds." Applied and Environmental Microbiology 73, no. 19 (August 3, 2007): 6012–18. http://dx.doi.org/10.1128/aem.00589-07.

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ABSTRACT A Saccharomyces cerevisiae strain, capable of autonomous bioluminescence, was engineered to respond to androgenic chemicals. The strain, S. cerevisiae BLYAS, contains the human androgen receptor in the chromosome and was constructed by inserting a series of androgen response elements between divergent yeast promoters GPD and ADH1 on pUTK401 that constitutively expressed luxA and luxB to create pUTK420. Cotransformation of this plasmid with a second plasmid (pUTK404), containing the genes required for aldehyde synthesis (luxCDE) and FMN reduction (frp), yielded a bioluminescent bioreporter responsive to androgenic chemicals. Using dihydrotestosterone (DHT) as a standard, the response time and the 50% effective concentration values were 3 to 4 h and (9.7 ± 4.6) × 10−9 M, respectively. The lower limit of detection in response to DHT was 2.5 × 10−9 M, and in response to testosterone it was 2.5 × 10−10 M. This strain is suitable for high-throughput screening of chemicals with potential for remote environmental monitoring systems because of the assay speed, sensitivity, and self-containment.

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Lukyanenko, Kirill, Ivan Denisov, Vladimir Sorokin, Anton Yakimov, Elena Esimbekova, and Peter Belobrov. "Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples." Chemosensors 7, no. 1 (March 26, 2019): 16. http://dx.doi.org/10.3390/chemosensors7010016.

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Enzymatic luminescent systems are a promising tool for rapid detection of heavy metals ions for water quality assessment. Nevertheless, their widespread use is limited by the lack of test procedure automation and available sensitive handheld luminometers. Herein we describe integration of disposable microfluidic chips for bioluminescent enzyme-inhibition based assay with a handheld luminometer, which detection system is based on a thermally stabilized silicon photomultiplier (SiPM). Microfluidic chips were made of poly(methyl methacrylate) by micro-milling method and sealed using a solvent bonding technique. The composition of the bioluminescent system in microfluidic chip was optimized to achieve higher luminescence intensity and storage time. Results indicate that developed device provided comparable sensitivity with bench-scale PMT-based commercial luminometers. Limit of detection for copper (II) sulfate reached 2.5 mg/L for developed biosensor. Hereby we proved the concept of handheld enzymatic optical biosensors with disposable chips for bioassay. The proposed biosensor can be used as an early warning field-deployable system for rapid detection of heavy metals salts and other toxic chemicals, which affect bioluminescent signal of enzymatic reaction.

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Cook, N., D. J. Silcock, R. N. Waterhouse, J. I. Prosser, L. A. Glover, and K. Killham. "Construction and detection of bioluminescent strains ofBacillus subtilis." Journal of Applied Bacteriology 75, no. 4 (October 1993): 350–59. http://dx.doi.org/10.1111/j.1365-2672.1993.tb02787.x.

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31

Kabiersch, Grit, Johanna Rajasärkkä, Marja Tuomela, Annele Hatakka, Marko Virta, and Kari Steffen. "Bioluminescent Yeast Assay for Detection of Organotin Compounds." Analytical Chemistry 85, no. 12 (May 28, 2013): 5740–45. http://dx.doi.org/10.1021/ac4003062.

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32

Girotti, Stefano, Luca Bolelli, Aldo Roda, Giovanna Gentilomi, and Monica Musiani. "Improved detection of toxic chemicals using bioluminescent bacteria." Analytica Chimica Acta 471, no. 1 (October 2002): 113–20. http://dx.doi.org/10.1016/s0003-2670(02)00870-x.

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Miller, S. D., S. H. D. Haddock, C. D. Elvidge, and T. F. Lee. "Detection of a bioluminescent milky sea from space." Proceedings of the National Academy of Sciences 102, no. 40 (September 26, 2005): 14181–84. http://dx.doi.org/10.1073/pnas.0507253102.

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Schofield, D. A., I. J. Molineux, and C. Westwater. "Diagnostic Bioluminescent Phage for Detection of Yersinia pestis." Journal of Clinical Microbiology 47, no. 12 (October 14, 2009): 3887–94. http://dx.doi.org/10.1128/jcm.01533-09.

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35

Ke, Bowen, Hui Chen, Lin Ma, Sarah Zingales, Deying Gong, Die Hu, Lupei Du, and Minyong Li. "Visualization of mercury(ii) accumulationin vivousing bioluminescence imaging with a highly selective probe." Organic & Biomolecular Chemistry 16, no. 14 (2018): 2388–92. http://dx.doi.org/10.1039/c8ob00398j.

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36

Siragusa, Gregory R., Kevin Nawotka, Stanley D. Spilman, Pamela R. Contag, and Christopher H. Contag. "Real-Time Monitoring of Escherichia coliO157:H7 Adherence to Beef Carcass Surface Tissues with a Bioluminescent Reporter." Applied and Environmental Microbiology 65, no. 4 (April 1, 1999): 1738–45. http://dx.doi.org/10.1128/aem.65.4.1738-1745.1999.

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ABSTRACT A method for studying bacteria that are attached to carcass surfaces would eliminate the need for exogenous sampling and would facilitate understanding the interaction of potential human food-borne pathogens with food animal tissue surfaces. We describe such a method in which we used a bioluminescent reporter strain of Escherichia coli O157:H7 that was constructed by transformation with plasmid pCGLS1, an expression vector that contains a complete bacterial luciferase (lux) operon. Beef carcass surface tissues were inoculated with the bioluminescent strain, and adherent bacteria were visualized in real time by using a sensitive photon-counting camera to obtain in situ images. The reporter strain was found to luminesce from the tissue surfaces whether it was inoculated as a suspension in buffer or as a suspension in a bovine fecal slurry. With this method, areas of tissues inoculated with the reporter strain could be studied without obtaining, excising, homogenizing, and culturing multiple samples from the tissue surface. Use of the complete lux operon as the bioluminescent reporter eliminated the need to add exogenous substrate. This allowed detection and quantitation of bacterial inocula and rapid evaluation of adherence of a potential human pathogen to tissue surfaces. Following simple water rinses of inoculated carcass tissues, the attachment duration varied with different carcass surface types. On average, the percent retention of bioluminescent signal from the reporter strain was higher on lean fascia-covered tissue (54%) than on adipose fascia-covered tissue (18%) following water washing of the tissues. Bioluminescence and culture-derived viable bacterial counts were highly correlated (r 2 = 0.98). Real-time assessment of microbial attachment to this complex menstruum should facilitate evaluation of carcass decontamination procedures and mechanistic studies of microbial contamination of beef carcass tissues.

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Joda, Hamdi, Angeliki Moutsiopoulou, Geoffrey Stone, Sylvia Daunert, and Sapna Deo. "Design of Gaussia luciferase-based bioluminescent stem-loop probe for sensitive detection of HIV-1 nucleic acids." Analyst 143, no. 14 (2018): 3374–81. http://dx.doi.org/10.1039/c8an00047f.

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38

Marques, Simone M., and Joaquim C. G. Esteves da Silva. "A nitric oxide quantitative assay by a glyceraldehyde 3-phosphate dehydrogenase/phosphoglycerate kinase/firefly luciferase optimized coupled bioluminescent assay." Anal. Methods 6, no. 11 (2014): 3741–50. http://dx.doi.org/10.1039/c4ay00317a.

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Noda, Natsumi, Tetsuya Ishimoto, Hisashi Mori, and Takeaki Ozawa. "Enhanced bioluminescent sensor for longitudinal detection of CREB activation in living cells." Photochemical & Photobiological Sciences 18, no. 11 (2019): 2740–47. http://dx.doi.org/10.1039/c9pp00249a.

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Yuan, Mingliang, Xiaojie Ma, Tianyu Jiang, Chaochao Zhang, Hui Chen, Yuqi Gao, Xingye Yang, Lupei Du, and Minyong Li. "A novel coelenterate luciferin-based luminescent probe for selective and sensitive detection of thiophenols." Organic & Biomolecular Chemistry 14, no. 43 (2016): 10267–74. http://dx.doi.org/10.1039/c6ob02038k.

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41

Yakimov, Anton S., Ivan A. Denisov, Anton S. Bukatin, Kirill A. Lukyanenko, Kirill I. Belousov, Igor V. Kukhtevich, Elena N. Esimbekova, Anatoly A. Evstrapov, and Peter I. Belobrov. "Droplet Microfluidic Device for Chemoenzymatic Sensing." Micromachines 13, no. 7 (July 20, 2022): 1146. http://dx.doi.org/10.3390/mi13071146.

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The rapid detection of pollutants in water can be performed with enzymatic probes, the catalytic light-emitting activity of which decreases in the presence of many types of pollutants. Herein, we present a microfluidic system for continuous chemoenzymatic biosensing that generates emulsion droplets containing two enzymes of the bacterial bioluminescent system (luciferase and NAD(P)H:FMN–oxidoreductase) with substrates required for the reaction. The developed chip generates “water-in-oil” emulsion droplets with a volume of 0.1 μL and a frequency of up to 12 drops per minute as well as provides the efficient mixing of reagents in droplets and their distancing. The bioluminescent signal from each individual droplet was measured by a photomultiplier tube with a signal-to-noise ratio of up to 3000/1. The intensity of the luminescence depended on the concentration of the copper sulfate with the limit of its detection of 5 μM. It was shown that bioluminescent enzymatic reactions could be carried out in droplet reactors in dispersed streams. The parameters and limitations required for the bioluminescent reaction to proceed were also studied. Hereby, chemoenzymatic sensing capabilities powered by a droplet microfluidics manipulation technique may serve as the basis for early-warning online water pollution systems.

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42

Engelen, Wouter, Kayleigh M. van de Wiel, Lenny H. H. Meijer, Bedabrata Saha, and Maarten Merkx. "Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids." Chemical Communications 53, no. 19 (2017): 2862–65. http://dx.doi.org/10.1039/c6cc10032e.

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Kudryavtsev, A. N., L. P. Burakova, and L. A. Frank. "Bioluminescent detection of tick-borne encephalitis virus in native ticks." Analytical Methods 9, no. 15 (2017): 2252–55. http://dx.doi.org/10.1039/c7ay00535k.

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Easy-to-use and fast bioluminescent immunoassay for tick-borne encephalitis virus in natural ticks based on the hybrid protein 14D5a-Rm7 was developed. The approach holds much promise with a view of practical applicability.

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44

Lopes, Nicholas, Shawn A. Hawkins, Patricia Jegier, Fu-Min Menn, Gary S. Sayler, and Steven Ripp. "Detection of dichloromethane with a bioluminescent (lux) bacterial bioreporter." Journal of Industrial Microbiology & Biotechnology 39, no. 1 (June 19, 2011): 45–53. http://dx.doi.org/10.1007/s10295-011-0997-5.

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Lin, Yuxing, Yuqi Gao, Zhao Ma, Zhenzhen Li, Chunchao Tang, Xiaojun Qin, Zheng Zhang, Guankai Wang, Lupei Du, and Minyong Li. "Bioluminescent Probe for Detection of Starvation-Induced Pantetheinase Upregulation." Analytical Chemistry 90, no. 15 (July 6, 2018): 9545–50. http://dx.doi.org/10.1021/acs.analchem.8b02266.

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Wong, Ling Shing, Simranjeet Kaur Judge, Betty Wan Niu Voon, Lee Jia Tee, Ka Yee Tan, Megalah Murti, and Mee Kin Chai. "Bioluminescent Microalgae-Based Biosensor for Metal Detection in Water." IEEE Sensors Journal 18, no. 5 (March 1, 2018): 2091–96. http://dx.doi.org/10.1109/jsen.2017.2787786.

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47

Fukuda, S., H. Tatsumi, H. Igarashi, and S. Igimi. "Rapid detection of Staphylococcus aureus using bioluminescent enzyme immunoassay." Letters in Applied Microbiology 31, no. 2 (August 2000): 134–38. http://dx.doi.org/10.1046/j.1365-2672.2000.00779.x.

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Akter, Farhima, Masayasu Mie, and Eiry Kobatake. "Aptamer-based protein detection using a bioluminescent fusion protein." Analyst 137, no. 22 (2012): 5297. http://dx.doi.org/10.1039/c2an35596e.

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49

Burakova, Ludmila P., Alexander N. Kudryavtsev, Galina A. Stepanyuk, Ivan K. Baykov, Vera V. Morozova, Nina V. Tikunova, Maria A. Dubova, Victor N. Lyapustin, Valeri V. Yakimenko, and Ludmila A. Frank. "Bioluminescent detection probe for tick-borne encephalitis virus immunoassay." Analytical and Bioanalytical Chemistry 407, no. 18 (April 30, 2015): 5417–23. http://dx.doi.org/10.1007/s00216-015-8710-6.

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50

BROVKO, LUBOV Yu, VALERY G. FROUNDJIAN, VERONIKA S. BABUNOVA, and NATALYA N. UGAROVA. "Quantitative assessment of bacterial contamination of raw milk using bioluminescence." Journal of Dairy Research 66, no. 4 (November 1999): 627–31. http://dx.doi.org/10.1017/s0022029999003817.

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Enumeration of bacteria in raw milk is of public health and economic importance. Among the proposed rapid methods for assessment of bacterial contamination in raw milk, ATP bioluminescence has proved to be one of the most promising (Griffiths, 1991). Several companies produce ATP bioluminescence reagent kits and equipment for analysing raw milk samples for total bacterial count (Sutherland et al. 1994; Reybroeck & Schram, 1995). The principle of ATP bioluminescent bacterial assay is based on the following assumptions (Olsen, 1991). All living organisms contain ATP, ATP is neither associated with dead cells nor absorbed on to surfaces, colloids and so on, and there is a fairly constant ratio of ATP to biomass/number of cells for all microbial taxa independent of metabolic activity or environmental conditions. Of these assumptions, only the first seems to be indisputable. It is not the number of bacterial cells, but rather the colony forming unit (cfu) that is the denomination used when assessing the microbial quality of milk. For Gram-negative rods of the genus Enterobacteriaceae, a cfu is usually derived from a single cell. However, Gram-positive cocci (staphylococci and streptococci) grow in bunches and chains respectively (Gregg, 1991), and estimation of cell numbers may not give good agreement with the colony counts.Several approaches have been investigated to increase the sensitivity of the bioluminescent method (Pahuski et al. 1991; Sutherland et al. 1994; Reybroeck & Schram, 1995; Froundjian et al. 1999). Although the detection limit achieved by these modifications (104 cfu/ml) may be sufficient for practical use (Bautista et al. 1992; Reybroeck & Schram 1995), the accuracy of the analysis was not significantly improved. The reported values for accuracy of the estimate for cfu/ml in raw milk (Syx) by the bioluminescent method were in the range 0·27–0·87 log units (Bautista et al. 1992; Reybroeck & Schram, 1995). The purpose of the present study was to determine the reasons for the lack of accuracy of the bacterial ATP assay in raw milk.

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