Relevant bibliographies by topics / Bioluminescence Vibrio fischeri

Academic literature on the topic 'Bioluminescence Vibrio fischeri'

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Published: 14 March 2023

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Journal articles on the topic "Bioluminescence Vibrio fischeri"

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Sarter, S., I. Metayer, and N. Zakhia. "Effects of mycotoxins, aflatoxin B1 and deoxynivalenol, on the bioluminescence of Vibrio fischeri." World Mycotoxin Journal 1, no. 2 (May 1, 2008): 189–93. http://dx.doi.org/10.3920/wmj2008.1011.

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The effects of aflatoxin B1 and deoxynivalenol on the luminescence of Vibrio fischeri were investigated to determine the conditions of using the bioluminescence as an indirect means for mycotoxin detection. The culture of Vibrio fischeri showed that bioluminescence reached a peak after 12 hours of incubation at 25 °C and then decreased drastically. During the lag phase which lasted 6 hours, light emission decreased drastically for both the mycotoxin assays – aflatoxin B1 10 µg/ml and deoxynivalenol 20 µg/ml – and the corresponding controls. Distinct bioluminescence inhibition appeared after this period of minimal bioluminescence of the controls and started with the exponential phase of growth. The percentage of bioluminescence inhibition for both mycotoxins was determined after 3.5, 10, 15 and 25 hours of incubation. The bioluminescence of Vibrio fischeri was inhibited with aflatoxin B1 and enhanced with deoxynivalenol. Both effects were delayed and required a long-term incubation over 10 hours, which may help to investigate bioassays for mycotoxin detection.
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Walker, Emma L., Jeffrey L. Bose, and Eric V. Stabb. "Photolyase Confers Resistance to UV Light but Does Not Contribute to the Symbiotic Benefit of Bioluminescence in Vibrio fischeri ES114." Applied and Environmental Microbiology 72, no. 10 (August 21, 2006): 6600–6606. http://dx.doi.org/10.1128/aem.01272-06.

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ABSTRACT Recent reports suggest that the selective advantage of bioluminescence for bacteria is mediated by light-dependent stimulation of photolyase to repair DNA lesions. Despite evidence for this model, photolyase mutants have not been characterized in a naturally bioluminescent bacterium, nor has this hypothesis been tested in bioluminescent bacteria under natural conditions. We have now characterized the photolyase encoded by phr in the bioluminescent bacterium Vibrio fischeri ES114. Consistent with Phr possessing photolyase activity, phr conferred light-dependent resistance to UV light. However, upon comparing ES114 to a phr mutant and a dark ΔluxCDABEG mutant, we found that bioluminescence did not detectably affect photolyase-mediated resistance to UV light. Addition of the light-stimulating autoinducer N-3-oxo-hexanoyl homoserine lactone appeared to increase UV resistance, but this was independent of photolyase or bioluminescence. Moreover, although bioluminescence confers an advantage for V. fischeri during colonization of its natural host, Euprymna scolopes, the phr mutant colonized this host to the same level as the wild type. Taken together, our results indicate that at least in V. fischeri strain ES114, the benefits of bioluminescence during symbiotic colonization are not mediated by photolyase, and although some UV resistance mechanism may be coregulated with bioluminescence, we found no evidence that light production benefits cells by stimulating photolyase in this strain.
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Yang, Xuepeng, Yan Ji, Fangfang Wang, Jia Xu, Xiangzhen Liu, Ke Ma, Xiangmei Hu, and Jianbin Ye. "Comparison of organics and heavy metals acute toxicities to Vibrio fischeri." Journal of the Serbian Chemical Society 81, no. 6 (2016): 697–705. http://dx.doi.org/10.2298/jsc151124011y.

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Vibrio fischeri bioluminescence inhibition has been widely used to test acute toxicities of metals and organics contaminants. However, the differences of metals and organics acute toxicities to V. fischeri have not been compared. Here, four heavy metals (Zn2+, Cu2+, Cd2+, Cr6+) and five organics (phenol, benzoic acid, p-hydroxy benzoic acid, nitro-benzene and benzene) acute toxicities to V. fischeri were investigated. Heavy metals toxicities to V. fischeri were increased along with the reaction time, while the organics toxicities kept the same level in different reaction times. In order to explain the difference, the relative cell death rate of V. fischeri was detected. In metals toxicities tests, the bioluminescence inhibition rate of V. fischeri was found to be significantly higher than the relative cell death rate (P<0.05), while for the organics toxicities tests, the cell death rate was similar to the bioluminescence inhibition rate. These results indicated that organics acute toxicities to V. fischeri could reflect the death of cell, but metals acute toxicities to V. fischeri may not lead to the death of cell, just represent the bioluminescence inhibition.
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Perry, Lynda L., Nathan G. Bright, Richard J. Carroll, Jr., M. Cathy Scott, Michael S. Allen, and Bruce M. Applegate. "Molecular characterization of autoinduction of bioluminescence in the Microtox® indicator strain Vibrio fischeri ATCC 49387." Canadian Journal of Microbiology 51, no. 7 (July 1, 2005): 549–57. http://dx.doi.org/10.1139/w05-019.

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Repeated attempts to clone the luxI from Vibrio fischeri ATCC 49387 failed to produce a clone carrying a functional LuxI. Sequence data from the clones revealed the presence of a polymorphism when compared with previously published luxI sequences, prompting further characterization of bioluminescence regulation in V. fischeri ATCC 49387. Further investigation of V. fischeri ATCC 49387 revealed that its LuxI protein lacks detectable LuxI activity due to the presence of a glutamine residue at position 125 in the deduced amino acid sequence. Specific bioluminescence in V. fischeri ATCC 49387 increases with increasing cell density, indicative of a typical autoinduction response. However, conditioned medium from this strain does not induce bioluminescence in an ATCC 49387 luxR-plux-based acyl homoserine lactone reporter strain, but does induce bioluminescence in ATCC 49387. It has been previously shown that a V. fischeri MJ-1 luxI mutant exhibits autoinduction of bioluminescence through N-octanoyl-L-homoserine lactone, the product of the AinS autoinducer synthase. However, a bioreporter based on luxR-plux from V. fischeri ATCC 49387 responded poorly to conditioned medium from V. fischeri ATCC 49387 and also responded poorly to authentic N-octanoyl-DL-homoserine lactone. A similar MJ-1-based bioreporter showed significant induction under the same conditions. A putative ainS gene cloned from ATCC 49387, unlike luxI from ATCC 49387, expresses V. fischeri autoinducer synthase activity in Escherichia coli. This study suggests that a regulatory mechanism independent of LuxR and LuxI but possibly involving AinS is responsible for the control of autoinduction of bioluminescence in V. fischeri ATCC 49387.Key words: quorum sensing, bioluminescence, Vibrio fischeri.
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Lee, John. "Fluorescent Antenna Proteins from the Bioluminescent Bacteria." Microscopy and Microanalysis 4, S2 (July 1998): 1002–3. http://dx.doi.org/10.1017/s1431927600025137.

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The emission spectrum from bioluminescent bacteria has been observed to depend on the type of bacteria. Photobacterium phosphoreum species usually show bioluminescence maxima around 472 nm and Photobacterium leiognathi species to slightly longer wavelength. A certain strain (Yl) of Vibrio fischeri, has a yellow bioluminescence with maximum at 542 nm. These differences have been explained as due to the bioluminescence originating from the fluorescence transition of an “antenna” protein, participating in the bioluminescence reaction along with the enzyme bacterial luciferase. The bioluminescence from a number of coelenterates involves a similar participation of an antenna protein, the famous “Green-Fluorescent Protein” being the origin of the bioluminescence emission from these organisms.
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Petrun, Branden, and C. Phoebe Lostroh. "Vibrio fischeriexhibit the growth advantage in stationary-phase phenotype." Canadian Journal of Microbiology 59, no. 2 (February 2013): 130–35. http://dx.doi.org/10.1139/cjm-2012-0439.

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Vibrio fischeri are bioluminescent marine bacteria that can be isolated from their symbiotic animal partners or from ocean water. A V. fischeri population increases exponentially inside the light organ of the Hawaiian bobtail squid (Euprymna scolopes) while the host is quiescent during the day. This bacterial light organ population reaches stationary phase and then remains high during the night, when the squid use bacterial bioluminescence as a counter-predation strategy. At dawn, host squid release 90%–95% of the light organ contents into the ocean water prior to burying in the sand for the day. As the squid sleeps, the cycle of bacterial population growth in the light organ begins again. These V. fischeri cells that are vented into the ocean must persist under typical marine low nutrient conditions until they encounter another opportunity to colonize a host. We hypothesized that because V. fischeri regularly encounter cycles of feast and famine in nature, they would exhibit the growth advantage in stationary phase (GASP) phenotype. We found that older V. fischeri cells exhibit a Class 2 GASP response in which old cells increase dramatically in frequency while the population of young V. fischeri cells remains almost constant during co-incubation.
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Silva, Ana Rita, Cláudia Sousa, Daniela Exner, Ruth Schwaiger, Maria Madalena Alves, Dmitri Y. Petrovykh, and Luciana Pereira. "pH-Induced Modulation of Vibrio fischeri Population Life Cycle." Chemosensors 9, no. 10 (October 5, 2021): 283. http://dx.doi.org/10.3390/chemosensors9100283.

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Commonly used as biological chemosensors in toxicity assays, Vibrio fischeri bacteria were systematically characterized using complementary physicochemical and biological techniques to elucidate the evolution of their properties under varying environmental conditions. Changing the pH above or below the optimal pH 7 was used to model the long-term stress that would be experienced by V. fischeri in environmental toxicology assays. The spectral shape of bioluminescence and cell-surface charge during the exponential growth phase were largely unaffected by pH changes. The pH-induced modulation of V. fischeri growth, monitored via the optical density (OD), was moderate. In contrast, the concomitant changes in the time-profiles of their bioluminescence, which is used as the readout in assays, were more significant. Imaging at discrete timepoints by scanning electron microscopy (SEM) and helium-ion microscopy (HIM) revealed that mature V. fischeri cells maintained a rod-shaped morphology with the average length of 2.2 ± 1 µm and diameter of 0.6 ± 0.1 µm. Detailed morphological analysis revealed subpopulations of rods having aspect ratios significantly larger than those of average individuals, suggesting the use of such elongated rods as an indicator of the multigenerational environmental stress. The observed modulation of bioluminescence and morphology supports the suitability of V. fischeri as biological chemosensors for both rapid and long-term assays, including under environmental conditions that can modify the physicochemical properties of novel anthropogenic pollutants, such as nanomaterials and especially stimulus-responsive nanomaterials.
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Wolfe, Alan J., Deborah S. Millikan, Joy M. Campbell, and Karen L. Visick. "Vibrio fischeri σ54 Controls Motility, Biofilm Formation, Luminescence, and Colonization." Applied and Environmental Microbiology 70, no. 4 (April 2004): 2520–24. http://dx.doi.org/10.1128/aem.70.4.2520-2524.2004.

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ABSTRACT In this study, we demonstrated that the putative Vibrio fischeri rpoN gene, which encodes σ54, controls flagellar biogenesis, biofilm development, and bioluminescence. We also show that rpoN plays a requisite role initiating the symbiotic association of V. fischeri with juveniles of the squid Euprymna scolopes.
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Pipes, Brian L., and Michele K. Nishiguchi. "Nocturnal Acidification: A Coordinating Cue in the Euprymna scolopes–Vibrio fischeri Symbiosis." International Journal of Molecular Sciences 23, no. 7 (March 29, 2022): 3743. http://dx.doi.org/10.3390/ijms23073743.

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The Vibrio fischeri–Euprymna scolopes symbiosis has become a powerful model for the study of specificity, initiation, and maintenance between beneficial bacteria and their eukaryotic partner. In this invertebrate model system, the bacterial symbionts are acquired every generation from the surrounding seawater by newly hatched squid. These symbionts colonize a specialized internal structure called the light organ, which they inhabit for the remainder of the host’s lifetime. The V. fischeri population grows and ebbs following a diel cycle, with high cell densities at night producing bioluminescence that helps the host avoid predation during its nocturnal activities. Rhythmic timing of the growth of the symbionts and their production of bioluminescence only at night is critical for maintaining the symbiosis. V. fischeri symbionts detect their population densities through a behavior termed quorum-sensing, where they secrete and detect concentrations of autoinducer molecules at high cell density when nocturnal production of bioluminescence begins. In this review, we discuss events that lead up to the nocturnal acidification of the light organ and the cues used for pre-adaptive behaviors that both host and symbiont have evolved. This host–bacterium cross talk is used to coordinate networks of regulatory signals (such as quorum-sensing and bioluminescence) that eventually provide a unique yet stable environment for V. fischeri to thrive and be maintained throughout its life history as a successful partner in this dynamic symbiosis.
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Halmi, Mohd Izuan Effendi, R. K. I. Phang, W. L. W. Johari, and M. Y. Shukor. "Toxicity Assessment of Bioluminescent Rapid Bioassays (Vibrio fischeri) on Selected DBPs." Journal of Environmental Microbiology and Toxicology 2, no. 2 (December 30, 2014): 47–52. http://dx.doi.org/10.54987/jemat.v2i2.169.

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For the past 30 years more than 600 different disinfection by-products (DBPs) have been reported with many unknown ones yet to be discovered. Bioluminescence rapid toxicity tests are suitable toxicity screening for DBPs that caused adverse health effects. Previously, IC50 study on specific DBPs have not been conducted. This study aims to characteristically identify the sensitivity of bioluminescent rapid bioassays on selected DBPs (chloroacetic acid, trichloroacetic acid, bromoacetic acid and iodoacetic acid) by measuring IC50 of Vibrio fischeri on these compounds. IC50 are determined through luminescence that was measured using a Beckman Counter DTX 800 multimode detector. The 30-minute IC50 of selected DBPs are as followed: CAA (865.4 mg/L), TCAA (1119 mg/L), BAA (59.67 mg/L) and IAA (15.6 mg/L). It was found that Bioluminescent Rapid Bioassays based on Vibrio fischeri showed promising sensitivity on the selected DBPs and are suitable as a screening tools for DBPs.
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Dissertations / Theses on the topic "Bioluminescence Vibrio fischeri"

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Ster, Ian M. "BinK Domain Functional Characterization in the Regulation of Bioluminescence in Vibrio Fischeri." Thesis, University of New Hampshire, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10688468.

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Prokaryotes encode a remarkable ability to adapt to niches by sensing environmental cues through signal transduction systems (STSs). Typical STS proteins interact through a phosphorylation relay between histidine (His) and aspartate (Asp) residues within modular domains on sensory kinase and response regulator (RR) proteins to elicit cellular responses. A single point mutation in the sensor kinase BinK (BinK1 R537C) conferred an outstanding ability for the non-native V. fischeri strain MJ11 to successfully colonize Euprymna scolopes by affecting multiple symbiotic phenotypes including luminescence activation. However, the role of BinK in luminescence, the interacting partners, and functional mechanism are unknown. We hypothesized that BinK interacts upstream of an orphaned RR and acts as a canonical sensor kinase using a C-terminal receiver (REC) domain to activate luminescence. Heterologous multi-copy expression of BinK in native V. fischeri strain ES114 demonstrated that BinK does not utilize an orphan RR, but instead interfaces with the LuxU-LuxO node to activate luminescence. Additionally, BinK with a truncated REC domain and a REC domain with an aspartate – alanine substitution abolished luminescence activation where the level of light emitted matched the level of light emitted by a strain harboring the empty vector plasmid, suggesting BinK activates luminescence in a REC-dependent manner using the conserved Asp residue for suspected phosphatase / dephosphorylation activity. Elimination of the kinase / auto-phosphorylation activity of the HisKA domain by incorporating a histidine–glutamine substitution did not alter BinK luminescence activation. Though these findings demonstrate one mechanism by which BinK activates luminescence, it is still not clear how the evolved binK1 R537C mutation in the HATPase catalytic domain, a domain important in kinase function, influences REC-dependent dephosphorylation. By using multi-copy expression, BinK1 reduces luminescence and increases qrr1 expression, and like BinK, works in a REC-dependent manner. These data suggest that one way BinK1 conferred the jump to symbiosis was through reduced or altered function. Furthermore, this mutation unveiled BinK as another potential regulator in bioluminescence where it is poised to work in a manner similar to quorum sensing activators AinR and LuxQ to activate luminescence.

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Silva, Gabriela Helena da. "Chronic effects of silica nanoparticles in Vibrio fischeri, Raphidocelis subcaptata, Danio rerio and Allium cepa." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-12122014-100850/.

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Scientific research using nanotechnology is a relatively recent development with a variety of potential applications in many fields of science. Within this field of research, many new products, with improved performances, have been developed. Despite increased research on its toxicity to ecosystem, the knowledge about this area is still limited. To evaluate the toxicity and genotoxicity of different sizes silica nanoparticles (SiNP)to the environment, different species, on different trophic levels (Vibrio fisheri, Raphidocelissubcapitata, Daniorerio and Allium cepa) were exposed to TM40 (22 nm), HS30 (12 nm), SM30 (7 nm) with concentrations ranging from0.19 to 163.8 g/L (TM40) and 0.29 to 122.85 g/L (HS30 and SM30), and the following parameters were monitored during exposure: production of bioluminescence (V. fischeri), growth rate (R. subcapitata), embryonic development and DNA damage (D. rerio) and germination rate, growth and DNA damage (A. cepa). Within each test SiNPpresent a size dependent chronic toxicity. The bioluminescence test present a EC50 of 29.11, 32.34 and 4.58 g/L for TM40, HS30 and SM30, respectively. For the growth rate assay the EC50 was 9.32, 9.07 and 7.93 g/L for TM40, HS30 and SM30, respectively. And for the zebra fish embryonic development test for TM40, HS30 and SM30, the EC50 was 5.85, 1.13 and 2.68 g/L respectively. All particles also induce phytotoxicity in A.cepa, growth and germination reduce significatively when expose to SiNP. Futhermoregenotoxic effects were also induced by the particles for both A.cepaand D. rerio. Therefore, SiNP can cause toxicity to the environment and size can strongly influence this toxicity
Com uma variedade de aplicações potenciais, em diversos campos da ciência, as pesquisas científicas utilizando nanotecnologia são de desenvolvimento relativamente recente. Dentro deste campo de pesquisa, vários novos produtos, com desempenhos melhorados têm sido desenvolvidos. Apesar do aumento de pesquisas sobre a toxicidade dessas tecnologias à biota, o conhecimento sobre esta área ainda é limitado. Visando avaliar a toxicidade e genotoxicidade denanopartículasde sílica (SiNP) no meio ambiente diferentes espécies pertencentes a diversos níveis tróficos (Vibriofisheri, Raphidocelissubcapitata, DaniorerioandAllium cepa) foram expostos a Ludox TM40 (22 nm), Ludox HS30 (12 nm) e Ludox SM30 (7 nm). As espécies de teste foram expostas a concentrações de nanopartículas (NP) variando de 0.29 a 163.8 g/L (TM40) e 0.19 a 122.85 g/L (HS30 e SM30) e os seguintes parâmetros monitorizados durante a exposição: a produção de bioluminescência (V. fischeri), o crescimento taxa (R. subcapitata), inibição de alimentação (D. magna), desenvolvimento embrionário e dano ao DNA (D. rerio) e taxa de germinação, crescimento e danos ao DNA (A. cepa). Nos testes feitos com as SiNPfoi observado que a toxicidade é dependente do tamanho da partícula. O ensaio de bioluminescência apresentou um EC50 de 29.11, 32.34 e 4.58 g/L para TM40, HS30 e SM30, respectivamente. Para o ensaio de taxa de crescimento o EC50 foi 9.32, 9.07 e 7.93 g/Lpara TM40, HS30 e SM30, respectivamente. E para o teste de desenvolvimento embrionário com peixe zebra, para o TM40, HS30 e SM30 o EC50 foi de 5.85, 1.13 e 2.68g/L, respectivamente. Todas as partículas também induziram fitotoxicidade em A. cepa, crescimento e germinação reduziram significativamente quando o organismo foi exposto a SiNP. Efeitos genotóxicos também foram induzir pelas partículas, tanto para A. cepa quanto paraD. rerio. Portanto, as SiNP podem causar toxicidade ao ambiente e o tamanho pode influenciar fortemente a essa toxicidade
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Hoffmann, Caroline C. "Investigation into the use of the Vibrio fischeri bioluminescence assay as a direct toxicity assessment (DTA) tool in the activated sludge environment." Thesis, Edinburgh Napier University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.326639.

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Alves, Eliana Sousa Cruz Ferreira. "Photodynamic inactivation of bacteria by cationic porphyrins : their cellular targets and potential environmental applications." Doctoral thesis, Universidade de Aveiro, 2013. http://hdl.handle.net/10773/12435.

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Doutoramento em Biologia
Photodynamic inactivation (PDI) is defined as the process of cell destruction by oxidative stress resulting from the interaction between light and a photosensitizer (PS), in the presence of molecular oxygen. PDI of bacteria has been extensively studied in recent years, proving to be a promising alternative to conventional antimicrobial agents for the treatment of superficial and localized infections. Moreover, the applicability of PDI goes far beyond the clinical field, as its potential use in water disinfection, using PS immobilized on solid supports, is currently under study. The aim of the first part of this work was to study the oxidative modifications in phospholipids, nucleic acids and proteins of Escherichia coli and Staphylococcus warneri, subjected to photodynamic treatment with cationic porphyrins. The aims of the second part of the work were to study the efficiency of PDI in aquaculture water and the influence of different physicalchemical parameters in this process, using the Gram-negative bioluminescent bacterium Vibrio fischeri, and to evaluate the possibility of recycling cationic PS immobilized on magnetic nanoparticles. To study the oxidative changes in membrane phospholipids, a lipidomic approach has been used, combining chromatographic techniques and mass spectrometry. The FOX2 assay was used to determine the concentration of lipid hydroperoxides generated after treatment. The oxidative modifications in the proteins were analyzed by one-dimensional polyacrylamide gel electrophoresis (SDS-PAGE). Changes in the intracellular nucleic acids were analyzed by agarose gel electrophoresis and the concentration of doublestranded DNA was determined by fluorimetry. The oxidative changes of bacterial PDI at the molecular level were analyzed by infrared spectroscopy. In laboratory tests, bacteria (108 CFU mL-1) were irradiated with white light (4.0 mW cm-2) after incubation with the PS (Tri-Py+-Me-PF or Tetra-Py+-Me) at concentrations of 0.5 and 5.0 μM for S. warneri and E. coli, respectively. Bacteria were irradiated with different light doses (up to 9.6 J cm-2 for S. warneri and up to 64.8 J cm-2 for E. coli) and the changes were evaluated throughout the irradiation time. In the study of phospholipids, only the porphyrin Tri-Py+-Me-PF and a light dose of 64.8 J cm-2 were tested. The efficiency of PDI in aquaculture has been evaluated in two different conditions: in buffer solution, varying temperature, pH, salinity and oxygen concentration, and in aquaculture water samples, to reproduce the conditions of PDI in situ. The kinetics of the process was determined in realtime during the experiments by measuring the bioluminescence of V. fischeri (107 CFU mL-1, corresponding to a level of bioluminescence of 105 relative light units). A concentration of 5.0 μM of Tri-Py+-Me-PF was used in the experiments with buffer solution, and 10 to 50 μM in the experiments with aquaculture water. Artificial white light (4.0 mW cm-2) and solar irradiation (40 mW cm-2) were used as light sources.
Os resultados deste trabalho mostraram que E. coli foi totalmente inativada com ambas as porfirinas, enquanto S. warneri foi completamente inativado apenas com a Tri-Py+-Me-PF, ao fim do tempo de irradiação previamente estabelecido. A IF induziu alterações no perfil fosfolipídico bacteriano, com aumento da abundância relativa de algumas das classes maioritárias de fosfolípidos, decréscimo de ácidos gordos insaturados, formação de espécies moleculares oxidadas a partir de ácidos gordos insaturados, nomeadamente nas cardiolipinas de S. warneri e nas fosfatidiletanolaminas de E. coli. Estas espécies oxidadas foram identificadas como derivados hidroxi e hidroperoxi (observados em E. coli) e também grupos carbonilo (em S. warneri). A formação de hidroperóxidos lipídicos confirmou os danos oxidativos nos fosfolípidos. A IF causou redução do conteúdo intracelular dos ácidos nucleicos bacterianos. Em E. coli observou-se a seguinte hierarquia de modificações: rRNA 23S > rRNA 16S > DNA genómico. Os ácidos nucleicos de S. warneri foram extensivamente reduzidos com a Tri-Py+-Me-PF após 5 min de irradiação, mas menos reduzidos com a Tetra-Py+-Me, após 40 min de irradiação. Esta degradação dos ácidos nucleicos ocorreu paralelamente à inativação e quando as células já estavam inativadas mais do que 99.9%. A IF induziu uma diminuição geral do conteúdo proteico de ambas as bactérias, sugerindo degradação em larga escala, ocorrendo as alterações de forma mais rápida e evidente com a porfirina Tri-Py+-Me-PF. Observou-se o aumento da expressão de algumas proteínas, alterações no peso molecular, desaparecimento após tratamento e formação de novas proteínas. As alterações foram associadas a mecanismos de resposta ao stress oxidativo. A espetroscopia de infravermelho mostrou ser um método rápido e económico de avaliar as alterações induzidas pela IF ao nível molecular. Evidenciou os resultados obtidos pelos métodos convencionais com maior detalhe, nomeadamente ao nível das ligações, grupos funcionais e conformações moleculares. As variações de pH (6.5 - 8.5), temperatura (10 - 25 ºC), salinidade (20 - 40 g L-1) e concentração de oxigénio não afetaram significativamente a IF de V. fischeri, uma vez que em todas as condições testadas o sinal bioluminescente diminuiu até ao limite de deteção do método (redução ≈ 7 log10). Os ensaios com água de aquacultura mostraram que a eficiência do processo é afetada pela presença de matéria em suspensão. A IF total de V. fischeri em água de aquacultura foi conseguida com luz solar na presença de 20 μM de Tri-Py+-Me-PF. Os híbridos nanomagnete-porfirina puderam ser reutilizados em 6 ciclos de IF e reciclados em 3 ciclos. Na reciclagem, houve perda de atividade de ciclo para ciclo, atribuída à perda de nanopartículas durante a recuperação. A acumulação de matéria orgânica causou uma redução da eficiência do processo durante a reutilização, contudo foi observada a eliminação de 38 a 42 log10 de bactérias ao fim de 21h30 a 27h de tratamento. O FS não foi fotodegradado e a magnetite das nanopartículas não foi afetada pela irradiação ou pela oxidação inerente ao processo fotodinâmico. O presente trabalho demonstrou o caráter multi-alvo da inativação fotodinâmica, pela elucidação dos mecanismos oxidativos que ocorrem ao nível dos principais constituintes moleculares das bactérias. Também demonstrou que a inativação fotodinâmica é uma metodologia com potencial para ser implementada na desinfeção de águas de aquacultura utilizando fotossensibilizadores imobilizados, permitindo a sua reutilização e reciclagem, com a possibilidade de reduzir os custos associados a este tipo de tratamento.
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CHIADO', ALESSANDRO. "Evaluation of new biorecognition elements for environmental monitoring." Doctoral thesis, Politecnico di Torino, 2013. http://hdl.handle.net/11583/2511708.

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To date, environmental monitoring is mainly focused on traditional chemical techniques, or on the assessment of specific biomarkers. However, these analyses are affected by several limitations: mainly, they are expensive, spot-sampling and time-consuming. In order to overcome these drawbacks, new biological monitoring methods, such as biosensors and biological early warning system (BEWS) are under development. These kinds of devices, built around whole cells, enzymes and antibodies, are well-suited to cooperatively and continuously monitor the environmental conditions. The key-factor of this very promising approach is the biological sensing element. Whole cell systems and enzymes are well suited for environmental monitoring: they are able to determine the bioavailable and toxic concentration of xenobiotics, especially if the source and nature of the compound cannot be predicted. Microorganisms usually detect a broad spectrum of chemicals, and represent a good opportunity for low cost, long shelf-life, and wide range of conditions in which they can be applied. Besides, enzymes are effective when a particular kind of pollutant would be detected because is possible to fine tune their metabolic behaviour by means of protein engineering. In this work, three biological sensing elements, related to three different index of toxicity were evaluated, in order to develop new biosensors for environmental monitoring: a broad toxicity index associated to the decrease of light emission (EC50 or half effective concentration) of a bioluminescent bacterium, Vibrio fischeri, a metal toxicity connected to the metal-regulated production of a siderophore (pyoverdine) by the soil and water microorganism Pseudomonas fluorescens, and finally an index of toxicity given by PAHs, was related to the metabolization of these compounds by laccase of Trametes versicolor. One of the first step during the assessment of a new biological sensing element is the study of the effect of physical-chemical parameters. The tested physical-chemical parameters (temperature, pH, inoculum percentage (v/v) and carbon source) influenced both microbial sensible elements (V. fischeri and P. fluorescens), therefore, these sensible elements can be used in a whole-cell biosensor for in-situ application, even if the response is affected by the environmental variables. Furthermore, the light emission of V. fischeri was highly variable, although a more stable bioluminescence was obtained by means of a glucose fed-batch: this is one step towards the direct application of this system, usually tailored for laboratory assays, to estimate the broad acute toxicity directly in situ in a portable device. Regarding the interaction between P. fluorescens and Fe3+, Cu2+, and Zn2+, the minimum inhibitory concentration (MIC) and the pyoverdine critical concentration (PCC) obtained values were compared to those indicated in the WHO Guidelines for drinking water quality and in European directive 98/83/EC: MICs of Fe3+, Cu2+ and Zn2+ are always above the threshold specified, whilst PCCs are very near to the recommended thresholds for iron and copper. The PCC was not determined for zinc in the tested range of concentration and conditions. These results highlighted that this sensible element should be further investigated for the development of a biosensor able to monitor metals in the environment. The last and most promising sensing element assessed in this work was the lccβ laccase of T. versicolor. A combination of computational docking (SwissDock) and molecular biology techniques was used to generate rationally engineered laccases with increased ability to process large and persistent PAHs. These mutated isoforms were produced by heterologous expression in P. pastoris, successfully purified, and characterized by means of biochemical assays. The activity of the enzymes was initially tested and characterized with phenolic and non phenolic substrates at different pH (3.0-8.0): the best mutated enzyme F162A/L164A (M1) showed an increased specific activity (UI/mg) in comparison with the wild type, in every tested condition. This result was in agreement with those obtained by computational docking simulations (estimated free binding energy), validating the rational design approach. Moreover, decolourization assays of large aromatic dyes, used as model compounds, have shown that the mutated enzymes are reactive towards molecules with chemical structure resembling that of aromatic organic pollutants. By means of example, enzyme mutants with a larger binding pocket (e.g. M1) showed higher activity against triphenylmethane dyes (e.g. Methyl Green), especially without a mediator of the reaction (HBT), and high stability under a variety of temperature conditions (4, 22 °C, room temperature). Therefore, the best enzyme should be integrated on an appropriate transducer (e.g. electrode), and coupled to a wireless platform generating a BEWS for environmental monitoring.
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Bose, Jeffrey L. "ArcA and FNR regulate bioluminescence in the light-organ symbiont Vibrio fischeri." 2007. http://purl.galileo.usg.edu/uga%5Fetd/bose%5Fjeffrey%5Fl%5F200708%5Fphd.

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Lupp, Claudia. "Quorum sensing in the Vibrio fisheri - Euprymna scolopes symbiosis /." 2003. http://hdl.handle.net/10125/1260.

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Chen, Hui-Yi, and 陳惠. "Gene Expression and Regulation of Bioluminescence: Functional Analysis of the Regulatory Gene of the lux Regulon form Vibrio fischeri." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/54920807387796787431.

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碩士
國立中興大學
分子生物研究所
81
For the purpose of simplicity, only the regulatory genes, luxR and luxI, were used for studying in this work. A series of gene order rearrangement constructions and in trans comple- mentary tests were designed to investigate the requirement of the sequences of the luxR and luxI genes, and functional analy- sis of the LuxR protein and autoinducer (AI). The luciferase genes, luxA-luxB, from V. harveyi were used as reporter gene to monitor the expression of the R- and L-promoter. The results show that the sequences of the luxR and luxI genes were the ne- gative control elements for the expression of the R- and L-pro- moter. The LuxR-AI complex is the positive control element for both the R- and L-promoter, and directly bound to the R&R se- quence. The sequence of the luxR gene repressed the expression of the L-promoter much stronger than the effect of the sequence of the luxI gene to the R-promoter. Nucleotide sequence analysis showed that an reversed repeat sequence, termed R3- reversed re- peat, was found on the sequence of the luxR gene, and it might be the LuxR motif-binding locus. The site-directed mutagenesis was used to inspect the function of this site. The results of the luxR mutations show that the premature translation of the LuxR protein repressed the expression of the following reporter genes. It suggested that the sequence of the luxR gene might re- gulate the lux regulon by attenuation-like termination. Further modification in this site was be done to define the function of the R3-reversed repeat, but the result can't confirm R3-reversed repeat is LuxR protein motif-binding locus for negative feedback control. The LuxR protein might bind on one specific site of the luxR sequence to enhance the attenuation-like transcriptional termination and negative feedback control the expression of L- operon.
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Books on the topic "Bioluminescence Vibrio fischeri"

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Callahan, Sean M. The quorum-sensing regulon of Vibrio fischeri: Novel components of the autoinducer/LuxR regulatory circuit. Cambridge, Mass: Massachusetts Institute of Technology, 1999.

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Callahan, Sean M. The quorum-sensing regulon of Vibrio fischeri: Novel components of the autoinducer/LuxR regulatory circuit. Cambridge, Mass: Massachusetts Institute of Technology, 1999.

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Wild, Ailsa, Aviva Reed, Briony Barr, Gregory Crocetti, and Linda Blackall. Squid, the Vibrio and the Moon. CSIRO Publishing, 2019. http://dx.doi.org/10.1071/9781486309900.

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When he hatches from his egg, Sepio, a baby bobtail squid, is not able to glow. His dark shape is too obvious in the moonlit water and all kinds of predators lurk nearby. Ali, an intrepid Vibrio fischeri bacterium, is determined to reach safety too. Can Ali and Sepio help each other? The Squid, the Vibrio and the Moon is a beautifully illustrated storybook about the symbiotic relationship between the Hawaiian bobtail squid and the bioluminescent bacteria that help it glow in the moonlight. Originally published in 2014, this book has been extensively re-written to delight and captivate primary-school aged readers.
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Book chapters on the topic "Bioluminescence Vibrio fischeri"

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Thomsen, Mette Ramsgaard, Martin Tamke, Aurelie Mosse, Jakob Sieder-Semlitsch, Hanae Bradshaw, Emil Fabritius Buchwald, and Maria Mosshammer. "Imprimer La Lumiere – 3D Printing Bioluminescence for Architectural Materiality." In Proceedings of the 2021 DigitalFUTURES, 305–15. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-5983-6_28.

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Abstract‘Imprimer la Lumière’ examines the making of a bioluminescent micro architecture. The project positions itself inside a sustainability agenda. By exploring the use of light-emitting bacteria as a material for architecture it asks what are the concepts, methods and technologies needed for designing with living materials. The project devises new means by which to design with the luminescent vibrio fischeri bacteria in a 3D printing manufacturing process based on extrusion principles. By combining the study of these living organisms and their appropriation through advanced robot-controlled 3D printing technologies, we establish a conceptual, material and technological framework for a bio-controlled bacteria growth and 3D extrusion process and a printable material based on agarose and gelatine.
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Pallaval Veera Bramhachari and G. Mohana Sheela. "Vibrio fischeri Symbiotically Synchronizes Bioluminescence in Marine Animals via Quorum Sensing Mechanism." In Implication of Quorum Sensing System in Biofilm Formation and Virulence, 207–19. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-2429-1_13.

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Thomas, Michael D., and Anita Van Tilburg. "Overexpression of foreign proteins using the Vibrio fischeri lux control system." In Bioluminescence and Chemiluminescence Part C, 315–29. Elsevier, 2000. http://dx.doi.org/10.1016/s0076-6879(00)05497-5.

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Devine, Jerry H., and Gerald S. Shadel. "Assay of autoinducer activity with luminescent Escherichia coli sensor strains harboring a modified Vibrio fischeri lux regulon." In Bioluminescence and Chemiluminescence Part C, 279–87. Elsevier, 2000. http://dx.doi.org/10.1016/s0076-6879(00)05494-x.

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Schaefer, Amy L., Brian L. Hanzelka, Matthew R. Parsek, and E. Peter Greenberg. "Detection, purification, and structural elucidation of the acylhomoserine lactone inducer of Vibrio fischeri luminescence and other related molecules." In Bioluminescence and Chemiluminescence Part C, 288–301. Elsevier, 2000. http://dx.doi.org/10.1016/s0076-6879(00)05495-1.

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Wakabayashi, Kenichi, and Masayuki Yamamura. "The Enterococcus faecalis Information Gate." In Cellular Computing. Oxford University Press, 2004. http://dx.doi.org/10.1093/oso/9780195155396.003.0011.

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Information exchange between cellular compartments allows us to engineer systems based around cooperative principles. In this chapter we consider a unique bacterial communication system, the conjugative plasmid transfer of Enterococcus faecalis. Using these bacteria, we describe how to engineer a logically controlled information gate and build a logical inverter based upon it. Cellular computing is an alternative computing paradigm based on living cells. Microscale organisms, especially bacteria, are well suited for computing for several reasons. A small culture provides an almost limitless supply of bacterial “hardware.” Bacteria can be stored and easily modified by gene recombination. In addition, and important for our purposes, bacteria can produce various signal molecules that are useful for computation. DNA-binding proteins recognize specific regulatory regions of DNA, bind them, and regulate their genetic expression. These proteins are available for use as computing signals inside the cell. Weiss et al. have shown, for example, how to construct logic circuits based on gene expression regulated by DNA-binding proteins. Some signal molecules are associated with intercellular communications between individuals. Intercellular communication is one of the fundamental characteristics of multicellular organisms, but it is also found in single-celled microorganisms, including bacteria. Communication mediated by homoserine lactones can widely be seen in various Gram-negative bacteria. The mechanism of this behavior was well characterized in Vibrio fischeri, due to their bioluminescent activity mediated by homoserine lactones. It has been shown that bacterial information transfer can be engineered as an extension of Escherichia coli into which the lux genes of Vibrio fischeri are transformed. The communication abilities of bacteria therefore allow us to build microbial information processors for cellular computing. Communication mechanisms in Gram-positive bacteria are not yet well understood. One of the exceptions to this is the conjugative plasmid transfer system in Enterococcus faecalis. E. faecalis conjugate in response to a pheromone is released by other cells. Pheromones are seven- or eight-residue amino peptides produced in E. faecalis. In the case of cPD1, the pheromone is produced by truncation of a 22-residue precursor that is the signal peptide of a lipoprotein.
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"Erratum: Vibrio fischeri: A Bioluminescent Light-Organ Symbiont of the Bobtail Squid Euprymna scolopes." In The Prokaryotes, E1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-30194-0_118.

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Conference papers on the topic "Bioluminescence Vibrio fischeri"

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ZRIMEC, M. BERDEN, and A. ZRIMEC. "ECOTOXICOLOGICAL SCREENING OF LJUBLJANA SURFACE WATERS WITH BIOLUMINESCENT BACTERIA VIBRIO FISCHERI (MICROTOX®)." In Bioluminescence and Chemiluminescence - Progress and Current Applications - 12th International Symposium on Bioluminescence (BL) and Chemiluminescence (CL). WORLD SCIENTIFIC, 2002. http://dx.doi.org/10.1142/9789812776624_0086.

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KARATANI, H., T. CHIBA, and S. HIRAYAMA. "RELATIONSHIP BETWEEN SPECTRAL DISTRIBUTION OF VIBRIO FISCHERI STRAIN Y1 BIOLUMINESCENCE AND INTRACELLULAR LEVEL OF ITS FLUORESCENT PROTEINS." In Bioluminescence and Chemiluminescence - Progress and Current Applications - 12th International Symposium on Bioluminescence (BL) and Chemiluminescence (CL). WORLD SCIENTIFIC, 2002. http://dx.doi.org/10.1142/9789812776624_0017.

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Novac, Laura, Catalina Stoica, Stefania Gheorghe, Irina Eugenia Lucaciu, and Mihai Nita-Lazar. "VIBRIO FISCHERI BIOLUMINESCENCE INHIBITION ASSAY FOR ACUTE TOXICITY PREDICTION OF PHARMACEUTICAL FROM WASTEWATERS AND SURFACE WATER: A REVIEW." In International Symposium "The Environment and the Industry". National Research and Development institute for Industrial Ecology, 2022. http://dx.doi.org/10.21698/simi.2022.ab17.

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