Relevant bibliographies by topics / Biological fluid analysis

Academic literature on the topic 'Biological fluid analysis'

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Published: 14 March 2023

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Journal articles on the topic "Biological fluid analysis"

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Terekhina, N. A., S. E. Reuk, and T. I. Atamanova. "Comparative analysis of ceruloplasmin level in biological fluids at herpes infection." Kazan medical journal 94, no. 5 (October 15, 2013): 752–54. http://dx.doi.org/10.17816/kmj1936.

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Aim. To compare the levels of ceruloplasmin in tears, saliva and blood serum of patients with herpetic stomatitis and eye herpes to evaluate the effectiveness of treatment. Methods. Ceruloplasmin levels were determined in tears, saliva and blood serum of 30 children, 22 adult patients with herpetic keratitis and 27 children with acute herpetic stomatitis. Biological fluids of 62 healthy individuals were used as the control group. Results. In patients with eye herpes infection, сeruloplasmin levels increased in oral fluid and blood serum and markedly decreased in tears of both affected and intact eye. Ceruloplasmin levels in biological fluids normalized only among children with light forms of eye herpes at discharge. In the case of acute herpetic stomatitis, ceruloplasmin levels increased in oral fluid and blood serum, depending on the severity of the disease. After the treatment, ceruloplasmin levels in tears, oral fluid and blood plasma normalized only in children with dendritic ulcer (herpes epithelial keratitis), while in adult patients with chronic relapsing eye herpes and in children with highly invasive eye herpes ceruloplasmin levels did not normalize. Conclusion. In the case of infection detected multidirectional ceruloplasmin levels in tears, oral fluids and blood serum changes were found in patients with herpes. Ceruloplasmin level decreased in tears, and increased in blood serum and oral fluid.
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Chaulin, A. M., L. S. Karslyan, E. V. Bazyuk, D. A. Nurbaltaeva, and D. V. Duplyakov. "Clinical and Diagnostic Value of Cardiac Markers in Human Biological Fluids." Kardiologiia 59, no. 11 (December 15, 2019): 66–75. http://dx.doi.org/10.18087/cardio.2019.11.n414.

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The article is devoted to problems of clinical-diagnostic value of determination of cardio-specific troponins in human biological fluids. Improvement of laboratory instrumentation and emergence of high sensitivity methods of analysis have allowed to identify troponins in urine, dialysate, and oral fluid. In the review we present actual information related to measurement of troponins in blood serum, data on testing of cardio-specific troponins in urine, dialysate, and oral fluid. Special attention is paid to determination of some cardiomarkers in oral fluid with thorough analysis of diagnostic value and effectiveness of the conducted studies.
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Kalíková, Květa, Denisa Folprechtová, and Zuzana Kadlecová. "Sub/supercritical Fluid Chromatography for Chiral Compounds Analysis." Chemické listy 116, no. 3 (March 15, 2022): 146–51. http://dx.doi.org/10.54779/chl20220146.

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Chirality is an essential feature of nature as it is common for many biologically active compounds. The different biological effects of individual enantiomers in a chiral environment are generally known. Therefore, there is a need for fast, efficient, and robust methods for their separation, quantification, and purification, too. The easiest way is to use chromatographic methods utilizing chiral stationary phases. Sub/supercritical fluid chromatography has become popular in the field of enantioselective separations in various scopes and, in some cases, has become a method of the first choice. Therefore, this review article covers actual trends and possibilities of sub/supercritical fluid chromatography in enantioseparations. Ways to influence enantioselectivity of the separation system by column coupling, screening approaches, and processes of methodical development for fast and efficient analyses are discussed. Sub/supercritical fluid chromatography under suitable experimental conditions provides fast and highly efficient separation of chiral compounds.
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NG, E. Y. K., DHANJOO N. GHISTA, and R. C. JEGATHESE. "NUMERICAL APPROACH TO FLUID-STRUCTURE ANALYSIS OF SOFT BIOLOGICAL TISSUE." Journal of Mechanics in Medicine and Biology 05, no. 01 (March 2005): 11–27. http://dx.doi.org/10.1142/s0219519405001278.

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Elasticity problems can be formulated into partial differential equations while analyzing the behavior of fluid-structure coupled problems. The current focus is to study the perfusion and instantaneous material property change in the soft tissues such as myocardium, which is analyzed due to the varying stiffness conditions. The analysis performed has considered physiological range loading conditions.
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Hoffmann, G., S. Aramaki, E. Blum-Hoffmann, W. L. Nyhan, and L. Sweetman. "Quantitative analysis for organic acids in biological samples: batch isolation followed by gas chromatographic-mass spectrometric analysis." Clinical Chemistry 35, no. 4 (April 1, 1989): 587–95. http://dx.doi.org/10.1093/clinchem/35.4.587.

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Abstract This new method for qualitative and quantitative determination of organic acids, aldehydes, and ketones in biological samples is effective for use with urine, plasma, and amniotic fluid, and it requires no deproteinization. Isolation by batch-wise liquid partition chromatography on silicic acid follows formation of the O-(2,3,4,5,6-pentafluorobenzyl)oximes of oxoacids, aldehydes, and ketones. The total organic acid content of the sample provides a rapid screening test for metabolic abnormality. A wide-bore, bonded-phase capillary column was used for quantitative gas chromatographic-mass spectrometric analysis, followed by automated identification and quantification. Analytical recoveries were quantitative for a wide variety of metabolites. Gas-chromatographic retention indices, discriminating ions, and control ranges in amniotic fluid, plasma, and urine of adult subjects were determined for 61 biologically important compounds.
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Garcia, Daisy C., Al Romero, Gerardo C. Garcia, and Enrique M. Ostrea. "Gastric Fluid Analysis for Determining Gestational Cocaine Exposure." Pediatrics 98, no. 2 (August 1, 1996): 291–93. http://dx.doi.org/10.1542/peds.98.2.291.

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Concerns for the acute and long-term complications associated with substance abuse during pregnancy have led to various ways of detecting gestational drug exposure in newborn infants. These have ranged from maternal history to the toxicologic analysis of biological samples. Maternal history is often unreliable because of maternal denial of drug use.1 Drug analysis of biological samples have included the analysis of hair, urine, amniotic fluid, and meconium. Hair analysis can provide information on the time and amount of drug use; however, technical problems inherent to the test have limited its use in the newborn period.2 Urine is often tested, but the incidence of false-negative tests is high.3
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Niu, Xize, and Andrew J. deMello. "Building droplet-based microfluidic systems for biological analysis." Biochemical Society Transactions 40, no. 4 (July 20, 2012): 615–23. http://dx.doi.org/10.1042/bst20120005.

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In the present paper, we review and discuss current developments and challenges in the field of droplet-based microfluidics. This discussion includes an assessment of the basic fluid dynamics of segmented flows, material requirements, fundamental unit operations and how integration of functional components can be applied to specific biological problems.
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Macêdo, Jéssica K. A., Joseph K. Joseph, Jaideep Menon, Teresa Escalante, Alexandra Rucavado, José María Gutiérrez, and Jay W. Fox. "Proteomic Analysis of Human Blister Fluids Following Envenomation by Three Snake Species in India: Differential Markers for Venom Mechanisms of Action." Toxins 11, no. 5 (April 30, 2019): 246. http://dx.doi.org/10.3390/toxins11050246.

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Skin blistering as a result of snakebite envenomation is characteristic of some bites, however little is known regarding the mechanism of blister formation or the composition of the blister fluid. In order to investigate if blister fluid proteomes from humans suffering snakebite envenomation could provide insights on the pathophysiology of these skin alterations, blister fluid was collected from six patients upon presentation at a clinic in India bitten by three species of snakes, Daboia russelii (3), Hypnale hypnale (2), or Naja naja (1). Standard clinical data were recorded throughout the treatment. Approximately 805 proteins were identified in blister fluids using proteomic analyses. Informatics analyses of the proteomes identified the top biological response categories as: platelet degranulation, innate immune response, receptor-mediated endocytosis, complement activation, and blood coagulation. Hierarchical clustering did not show a clear segregation of patients’ proteomes being associated with the species of snake involved, suggesting that either the proteomic profiles described reflect a general response to venom-induced tissue damage or more patient data sets will be required to observe significant differences. Finally, it is of interest that venom proteins were also identified in the blister fluids suggesting that this fluid may serve as a reservoir of venom biologically active proteins/toxins, and as such, may indicate the clinical value of removing blister fluid to attenuate further tissue damage.
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Hanson, Erin K., and Jack Ballantyne. "Rapid and inexpensive body fluid identification by RNA profiling-based multiplex High Resolution Melt (HRM) analysis." F1000Research 2 (December 20, 2013): 281. http://dx.doi.org/10.12688/f1000research.2-281.v1.

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Positive identification of the nature of biological material present on evidentiary items can be crucial for understanding the circumstances surrounding a crime. However, traditional protein-based methods do not permit the identification of all body fluids and tissues, and thus molecular based strategies for the conclusive identification of all forensically relevant biological fluids and tissues need to be developed. Messenger RNA (mRNA) profiling is an example of such a molecular-based approach. Current mRNA body fluid identification assays involve capillary electrophoresis (CE) or quantitative RT-PCR (qRT-PCR) platforms, each with its own limitations. Both platforms require the use of expensive fluorescently labeled primers or probes. CE-based assays require separate amplification and detection steps thus increasing the analysis time. For qRT-PCR assays, only 3-4 markers can be included in a single reaction since each requires a different fluorescent dye.To simplify mRNA profiling assays, and reduce the time and cost of analysis, we have developed single- and multiplex body fluid High Resolution Melt (HRM) assays for the identification of common forensically relevant biological fluids and tissues. The incorporated biomarkers include IL19 (vaginal secretions), IL1F7 (skin), ALAS2 (blood), MMP10 (menstrual blood), HTN3 (saliva) and TGM4 (semen). The HRM assays require only unlabeled PCR primers and a single saturating intercalating fluorescent dye (Eva Green). Each body-fluid-specific marker can easily be identified by the presence of a distinct melt peak. Usually, HRM assays are used to detect variants or isoforms for a single gene target. However, we have uniquely developed duplex and triplex HRM assays to permit the simultaneous detection of multiple targets per reaction. Here we describe the development and initial performance evaluation of the developed HRM assays. The results demonstrate the potential use of HRM assays for rapid, and relatively inexpensive, screening of biological evidence.
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Hanson, Erin K., and Jack Ballantyne. "Rapid and inexpensive body fluid identification by RNA profiling-based multiplex High Resolution Melt (HRM) analysis." F1000Research 2 (February 26, 2014): 281. http://dx.doi.org/10.12688/f1000research.2-281.v2.

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Positive identification of the nature of biological material present on evidentiary items can be crucial for understanding the circumstances surrounding a crime. However, traditional protein-based methods do not permit the identification of all body fluids and tissues, and thus molecular based strategies for the conclusive identification of all forensically relevant biological fluids and tissues need to be developed. Messenger RNA (mRNA) profiling is an example of such a molecular-based approach. Current mRNA body fluid identification assays involve capillary electrophoresis (CE) or quantitative RT-PCR (qRT-PCR) platforms, each with its own limitations. Both platforms require the use of expensive fluorescently labeled primers or probes. CE-based assays require separate amplification and detection steps thus increasing the analysis time. For qRT-PCR assays, only 3-4 markers can be included in a single reaction since each requires a different fluorescent dye.To simplify mRNA profiling assays, and reduce the time and cost of analysis, we have developed single- and multiplex body fluid High Resolution Melt (HRM) assays for the identification of common forensically relevant biological fluids and tissues. The incorporated biomarkers include IL19 (vaginal secretions), IL1F7 (skin), ALAS2 (blood), MMP10 (menstrual blood), HTN3 (saliva) and TGM4 (semen). The HRM assays require only unlabeled PCR primers and a single saturating intercalating fluorescent dye (Eva Green). Each body-fluid-specific marker can easily be identified by the presence of a distinct melt peak. Usually, HRM assays are used to detect variants or isoforms for a single gene target. However, we have uniquely developed duplex and triplex HRM assays to permit the simultaneous detection of multiple targets per reaction. Here we describe the development and initial performance evaluation of the developed HRM assays. The results demonstrate the potential use of HRM assays for rapid, and relatively inexpensive, screening of biological evidence.
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Dissertations / Theses on the topic "Biological fluid analysis"

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Gurekian, Christine N. "Amniotic fluid amino acids as biological indicators of fetal growth in human and rat models." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=98718.

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Amniotic fluid (AF) is a protective pool and a resource of amino acids for the growing fetus. In study 1, we investigated if any of these AF amino acids at mid gestation were associated with fetal development in humans. Nineteen amino acids differed across birth weight percentiles. Arginine, 3-methyl histidine and tryptophan were positive predictors of birth weight, while ornithine was a negative predictor. In study 2, we used a diet induced model of IUGR to see if specific AF amino acids were predictive of fetal weight near term. Methionine and phenylalanine were modified by diet, and 12 amino acids were independently modified by gestational age, respectively. Cysteine, lysine, methionine and tyrosine were predictors of fetal weight. Thus, the AF amino acid pool is associated in animals and humans with fetal growth.
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Damon, Deidre Erin. "Development of Functionalized Paper-Based Sample Collection and Direct Mass Spectrometry Analysis Platforms." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1550776934984565.

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Ramström, Margareta. "Analysis of Complex Biological Samples using Liquid Chromatography-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry." Doctoral thesis, Uppsala University, Analytical Chemistry, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5729.

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Studies of protein and peptide expression are vital in order to understand complex biological systems. As demonstrated in this thesis, on-line packed capillary liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR MS) is a useful analytical tool for such studies.

A proteomics method, based on global tryptic digestion and subsequent separation and detection of the peptides by LC-FTICR MS, was developed for qualitative analysis of body fluids. Initial experiments on cerebrospinal fluid (CSF) provided results that were comparable or superior to those achieved by more time- and sample-consuming techniques. The method was also successfully applied on plasma and amniotic fluid. One of the major challenges in proteomics is the broad dynamic range of proteins in biological matrices. The advantages of removing high-abundant components from CSF and plasma prior to MS were demonstrated.

In order to search for potential biomarkers, mass chromatograms of CSF from patients suffering from amyotrophic lateral sclerosis (ALS) and controls were compared using an in-house constructed pattern recognition program. ALS-specific patterns were observed, and four out of five unknown samples were correctly assigned. Alternative strategies to quantitatively compare two pools of samples rely on differential chemical labeling. The performance of one such method, quantification-using-enhanced-signal-tags, was investigated in complex sample analysis. The experimental intensity ratios were proven to be consistent with the prepared concentration ratios of abundant proteins in CSF.

Finally, the thesis reports on the first experiments where electron capture dissociation (ECD) was successfully incorporated in on-line LC-MS experiments. ECD and nozzle-skimmer fragmentation were applied to a sample of endocrine peptides extracted from mouse pancreatic islets. The two fragmentation methods provided complementary information. However, the method needs further optimization before it can be applied in the analysis of more complex samples, such as body fluids.

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Bergseije, Victor. "Effects of Heat Transfer Fluid from District Heating Networks on Activated Sludge : A respirometric analysis using a dilution series to assess disruption of biological treatment processes in wastewater treatment facilities." Thesis, Linnéuniversitetet, Institutionen för biologi och miljö (BOM), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-34038.

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District heating has a long standing tradition in Sweden and today it is the most common way of producing and transporting heat. A District heating system (DH system) is divided into three parts: a production facility, distribution network (DH network) and one more heat stations. The heat produced in the facilities is distributed to the customers via a heat transfer medium, usually water (DH water), in piping networks that make up the DH network. The heat is transferred to the customers via the heat exchanger at which point they can use it as heated tap water or for heating purposes. The DH networks are often constructed in steel as it is cheap and a relatively resistant material. However it has the disadvantages of corrosion and expansions when it is exposed high temperatures which lead to damages in the DH network resulting in loss of the DH water, this is an unavoidable occurrence in any DH network. This results in addition of pollutants by leakages into the DH network or with the water that is used to compensate for the losses. The pollutants cause further corrosion, leading to metal contamination, and more damages on the DH network meaning there is a continuous degradation. Therefore various treatments are used to clean and ascertain an acceptable chemical environment in the DH systems. These treatments are effective but not at a level which is required so many chemicals are used to enhance the treatment of the water. Some of these are known to be toxic to humans and water ecosystems. As leakages are abundant and often end up in the WWTPs of the concerned municipality, which often have troubles with disturbances of the biological treatment, it was decided that an assessment of the toxic effects that DH water pose on activated sludge was to be investigated. This was done by testing water from two DH networks, Växjö and Kalmar, on the same activated sludge obtained from Tegelviken WWTP in Kalmar. A respirometric bioassay approach established by the Organization for Economic Co-operation and Development (OECD), OECD standard 209; OECD Guidelines for the Testing of Chemicals was used with changes made to exposure and measuring time as this decrease the risk of misinterpretation of the results. A dilution series using different concentrations (6.25%, 25% and 100%) of DH water was tested and compered to a blank control samples containing only activated sludge. Assessment of toxicity on total oxidation, oxidation carbon and oxidation of nitrogen was made. To get some idea of what might cause toxic effect samples of the waters was sent to outside laboratories for analyses of metals. The result from the bioassay and metal analysis was used to formulate risk factors associated with a DH water spill and exposure to WWTPs. It was found that both DH waters have a significant inhibition on nitrification in WWTPs. The DH water from Kalmar exhibited similar toxicity dynamics, roughly 20% inhibition, despite large differences in concentration. The DH water from Växjö showed a negative correlation between an increase in concentration of DH water and toxicity, 74% for the lowest concentration and 11% for the highest. The metal analysis concluded that there was no abundance of metal contamination which led to the inference that toxicity is probably caused by the chemicals used for treatment. This poses a great risk for the Baltic Ocean as many WWTPs release their treated water directly into water courses with a short detention time before reaching the sea.
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Заболотна, Наталія Іванівна. "Багатопараметричні поляризаційно-фазові методи і засоби відтворення та аналізу структури полікристалічних біологічних шарів при оцінюванні патологічних станів." Thesis, Вінницький національний технічний університет, 2018. http://repository.kpi.kharkov.ua/handle/KhPI-Press/38209.

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Дисертація на здобуття наукового ступеня доктора технічних наук за спеціальністю 05.11.17 – біологічні та медичні прилади i системи. – Національний технічний університет "Харківський політехнічний інститут", Харків, 2018. Розв'язано комплекс задач, які вирішили науково-прикладну проблему створення теоретичних засад, методів і засобів багатопараметричного поляризаційного відтворення та об'єктивного аналізу структури фазово-неоднорідних біологічних об'єктів з підвищенням достовірності оцінювання патологічних станів в системах діагностики гістологічних зрізів парціальних і двошарових біологічних тканин і плівок біологічних рідин. Запропоновано модель відтворення та аналізу оптичної анізотропії багатошарових біологічних тканин і рідин із виділенням груп їх мюллер-матричних зображень при оцінюванні патологічних станів. Розроблено методи і системи з підвищеною достовірністю диференціації станів "норма – патологія" на основі прямого відтворення та аналізу координатних розподілів орієнтаційних та фазових параметрів оптично тонких біологічних шарів та мюллер-матричного відтворення "екранованих" зовні шарів двошарової біологічної тканини. Розроблена та апробована архітектура багатопараметричної системи поляризаційно-фазового відтворення та аналізу параметрів анізотропії біологічних шарів з розширеними функціональними можливостями та підвищеною достовірністю діагностування патологічних станів. Оцінено метрологічні характеристики запропонованих систем на основі статистичного, кореляційного та фрактального аналізу двомірних розподілів похибок вимірювання.
Dissertation for a Doctor`s of Science (Engineering) Degree on Specialty 05.11.17 – Biological and Medical Devices and Systems. – Vinnytsia National Technical University, National Technical University "Kharkiv Polytechnic Institute", Kharkiv, 2018. Dissertation is dedicated to the solution of the scientific-applied problem, aimed at elaboration of theoretical fundamentals, methods and means of multiparameter polarization reconstruction and unbiased analysis of the phaseheterogeneous biological objects structure that enabled to enhance the validity of pathological states assessment in the diagnostic systems of the histologic sections of fractional and multilayered biological tissues (BT) and films of biological fluids (BF). The model of reconstruction and analysis of optical anisotropy of multilayer BT and BF with the allocation of groups of their mueller-matrix images in the evaluation of pathological states is improved. Methods and systems with increased reliability of the differentiation of states "norm – pathology" on the basis of direct reproduction and analysis of coordinate distributions of orientation and phase parameters of optically thin biological layers (BL) and mueller-matrix reproduction of "shielded" exterior layers of two-layer BT are developed. The architecture of the multiparameter system of polarization-phase reproduction and analysis of parameters of anisotropy of BL with advanced functional capabilities and high reliability of diagnostics of pathological states was developed and tested. The metrological characteristics of the offered systems based on statistical, correlation and fractal analysis of two-dimensional distributions of measurement errors are estimated.
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Kapita, Patrick Mvemba. "Development of Measurement Systems for Biosensing Applications." Doctoral thesis, Università di Siena, 2020. http://hdl.handle.net/11365/1111250.

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A health condition called “Oxidative Stress” (OS), resulting from an excessive level of Reactive Oxygen Species (ROS) is a “state harmful to the body, which arises when oxidative reactions exceed antioxidant reactions because the balance between them has been lost”[1] OS appears to be associated with and might be a cause of, many serious diseases such as cardio-vascular accidents, cancer, Parkinson’s and Alzheimer’s[2]. This is not surprising as ROS are free oxygen radicals that can attack lipids, proteins, cellular membranes, enzymes and even modify DNA. Extensive correlation studies have shown that the complex impedance spectrum of blood samples from patients diagnosed with an OS syndrome differs significantly from the spectra obtained from the blood of healthy people, which is quite normal as the presence of an excessive amount of ROS should affect the physico-chemical properties of a blood sample. Measuring the complex impedance spectrum of a blood sample can be done quickly by means of low-cost electronic devices, making possible and affordable the early detection of OS among a large population. In order to quantitatively evaluate the OS, the impedance spectra being insufficient, the concentration of oxidative stress markers such as hydrogen peroxyde, malondialdehyde or F2 isoprostanes needs to be measured. Such measurements can, for instance, be used for monitoring the severity of a disease during a treatment. These concentration measurements are traditionally based upon analytical techniques but recently biosensors acting as transducers transforming directly a specific biochemical reaction into a measurable signal have been developed. They are essentially obtained by modifying the surface of metal or carbon electrodes using biomaterials such as enzymes antibodies or DNA that allow bindings or catalytic reactions with other specific biomaterials to occur on the surface of the electrodes. The resulting modifications of the electrical properties of the medium separating the electrodes can be analyzed through ad-hoc electronic and signal processing systems to yield the desired concentration. Biosensors have the advantages of rapid analysis, low-ost and high-precision. They are widely used in various fields, such as medical care, disease diagnosis and food analysis [3]. Hydrogen peroxide (H2O2) generated by cellular processes directly via two-electron reduction of molecular oxygen or indirectly via dismutation of superoxide, is the most widely studied ROS and its overproduction results in OS. Therefore, an ability to quantify the level of hydrogen peroxide and by ricochet the assessment of oxidative stress can be useful in order to assess certain health conditions occurring inside the body and as a result, an integrated electrochemical biosensor coupled with the hydrogen peroxide quantification can become a practical solution as a point of care device at home[4] Most of the time, H2O2 biosensors are based on HRP (Horseradish peroxidase) which is the most commonly used enzyme in the design of biosensors that can supervise the activity of oxidases and determine in terms of concentration, oxidase substrate such as lactate oxidase, cholesterol oxidase, or glucose oxidase, which all induce the production of hydrogen peroxide (HRP’s substrate). In the first part of this research, we explore the development of low-cost and compact measurement systems aiming to determining the impedance of biological samples as they grant access to information from electrical cellular characteristics. It is indeed possible to measure capacitance or conductance that are dependent on the health state of cells. The development of such measurement systems allowing the portability of biological essays requires sensitive electronics. Afterward, in the second part of our work, we explore the design of an electrochemical biosensor by immobilizing an enzyme (HRP) onto the surface of golden electrodes in order to detect and assess the analyte, hydrogen peroxide (H2O2). We also discuss the design of a potentiostat readout circuit to measure and convert the biosensor’s current. The combined results of the two parts of this work can be considered as a first prototype of a low cost and robust instrument easy to use in the field, away from a biological laboratory, with the goal of reaching the so called “point of care diagnostic” [5] The present thesis is organized as follows: Chapter I, introduces the present thesis. In Chapter II, we provide an overview in the field of biosensing technology. Chapter III deals with the design of a portable EIS measurement system to investigate reactive oxygen species in blood. Chapter IV presents an improved version of the previously designed instrument. Moreover, it points out the significance of EIS-based blood analysis through relevant medical diagnosis parameters such as hematocrit and erythrocyte sedimentation rate, extracted from the measured impedance spectra. In Chapter V we discuss on one hand the design of the H2O2 biosensor, and on the other hand the realization of the front-end circuit of the amperometric sensor. Finally, in Chapter VI, a conclusion is drawn..
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Заболотна, Наталія Іванівна. "Багатопараметричні поляризаційно-фазові методи і засоби відтворення та аналізу структури полікристалічних біологічних шарів при оцінюванні патологічних станів." Thesis, НТУ "ХПІ", 2018. http://repository.kpi.kharkov.ua/handle/KhPI-Press/38206.

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Дисертація на здобуття наукового ступеня доктора технічних наук за спеціальністю 05.11.17 – біологічні та медичні прилади i системи. – Національний технічний університет "Харківський політехнічний інститут", Харків, 2018. Розв'язано комплекс задач, які вирішили науково-прикладну проблему створення теоретичних засад, методів і засобів багатопараметричного поляризаційного відтворення та об'єктивного аналізу структури фазово-неоднорідних біологічних об'єктів з підвищенням достовірності оцінювання патологічних станів в системах діагностики гістологічних зрізів парціальних і двошарових біологічних тканин і плівок біологічних рідин. Запропоновано модель відтворення та аналізу оптичної анізотропії багатошарових біологічних тканин і рідин із виділенням груп їх мюллер-матричних зображень при оцінюванні патологічних станів. Розроблено методи і системи з підвищеною достовірністю диференціації станів "норма – патологія" на основі прямого відтворення та аналізу координатних розподілів орієнтаційних та фазових параметрів оптично тонких біологічних шарів та мюллер-матричного відтворення "екранованих" зовні шарів двошарової біологічної тканини. Розроблена та апробована архітектура багатопараметричної системи поляризаційно-фазового відтворення та аналізу параметрів анізотропії біологічних шарів з розширеними функціональними можливостями та підвищеною достовірністю діагностування патологічних станів. Оцінено метрологічні характеристики запропонованих систем на основі статистичного, кореляційного та фрактального аналізу двомірних розподілів похибок вимірювання.
Dissertation for a Doctor`s of Science (Engineering) Degree on Specialty 05.11.17 – Biological and Medical Devices and Systems. – Vinnytsia National Technical University, National Technical University "Kharkiv Polytechnic Institute", Kharkiv, 2018. Dissertation is dedicated to the solution of the scientific-applied problem, aimed at elaboration of theoretical fundamentals, methods and means of multiparameter polarization reconstruction and unbiased analysis of the phaseheterogeneous biological objects structure that enabled to enhance the validity of pathological states assessment in the diagnostic systems of the histologic sections of fractional and multilayered biological tissues (BT) and films of biological fluids (BF). The model of reconstruction and analysis of optical anisotropy of multilayer BT and BF with the allocation of groups of their mueller-matrix images in the evaluation of pathological states is improved. Methods and systems with increased reliability of the differentiation of states "norm – pathology" on the basis of direct reproduction and analysis of coordinate distributions of orientation and phase parameters of optically thin biological layers (BL) and mueller-matrix reproduction of "shielded" exterior layers of two-layer BT are developed. The architecture of the multiparameter system of polarization-phase reproduction and analysis of parameters of anisotropy of BL with advanced functional capabilities and high reliability of diagnostics of pathological states was developed and tested. The metrological characteristics of the offered systems based on statistical, correlation and fractal analysis of two-dimensional distributions of measurement errors are estimated.
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Alford, Lionel Devon Jr. "Aerodynamic Analysis of Natural Flapping Flight Using a Lift Model Based on Spanwise Flow." University of Dayton / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1272639883.

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Kelly, Barbara M. "The analysis of biological fluids for acylcarnitines." Thesis, Open University, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.326566.

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Kaspar, Hannelore. "Amino acid analysis in biological fluids by GC-MS." kostenfrei, 2009. http://www.opus-bayern.de/uni-regensburg/volltexte/2009/1316/.

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Books on the topic "Biological fluid analysis"

1

M, Lazaro Deana, ed. Analysis of synovial fluid. Summit, N.J: CIBA-GEIGY, 1992.

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Vasilʹevich, Priezzhev Aleksandr, Coté Gerard L, and Society of Photo-optical Instrumentation Engineers., eds. Optical diagnostics and sensing of biological fluids and glucose and cholesterol monitoring: 22-23 January 2001, San Jose, USA. Bellingham, Wash., USA: SPIE, 2001.

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Vasilʹevich, Priezzhev Aleksandr, Coté Gerard Laurence, and Society of Photo-optical Instrumentation Engineers., eds. Optical diagnostics and sensing of biological fluids and glucose and cholesterol monitoring II: 23-24 January 2002, San Jose, USA. Bellingham, Wash., USA: SPIE, 2002.

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Fundamentals of microfluidics and lab on a chip for biological analysis and discovery. Boca Raton: Taylor & Francis, 2010.

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Symposium, OO "Materials and Strategies for Lab-on-a.-Chip-Biological Analysis Cell-Material Interfaces and Fluidic Assembly of Nanostructures" (2009 San Francisco Calif ). Materials and strategies for lab-on-a-chip--biological analysis, cell-material interfaces, and fluidic assembly of nanostructures: Symposium held April 14-17, 2009, San Francisco, California, U.S.A. Warrendale, Pa: Materials Research Society, 2009.

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Analysis of drugs in biological fluids. Boca Raton, Fla: CRC Press, 1985.

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Chamberlain, Joseph. The analysis of drugs in biological fluids. 2nd ed. Boca Raton: CRC Press, 1995.

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Hormone assays in biological fluids. New York: Humana Press, 2013.

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J, Wheeler M., and Hutchinson J. S. M, eds. Hormone assays in biological fluids. Totowa, N.J: Humana Press, 2006.

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1942-, Berthon Guy, ed. Handbook of metal-ligand interactions in biological fluids. New York: Marcel Dekker, 1995.

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Book chapters on the topic "Biological fluid analysis"

1

Mazwan Mahat, M., A. Juliawati, and Ishkrizat Taib. "Biomechanical Modeling of Aneurysm Growth and Rupture Using Fluid Structure Interaction." In Analysis and Design of Biological Materials and Structures, 151–61. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-22131-6_12.

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Jackson, Michael, Hans H. Eysel, R. Anthony Shaw, Glen T. D. Thomson, and Henry H. Mantsch. "Non-Subjective Diagnosis of Arthritic Disorders by Multivariate Analysis of IR Spectra of Synovial Fluid." In Spectroscopy of Biological Molecules, 499–500. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0371-8_229.

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Slobozhanina, Ekaterina I., Eugene D. Beloyenko, Nataly M. Kozlova, and Eugene A. Chernitsky. "Spectral luminescence analysis of synovial fluid in diagnostic of chronic joint diseases." In Spectroscopy of Biological Molecules: New Directions, 525–26. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4479-7_236.

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Shukla, Snehal, and Gunamani Deheri. "Effect of Slip Velocity on the Performance of a Magnetic Fluid Based Transversely Rough Porous Narrow Journal Bearing." In Applied Analysis in Biological and Physical Sciences, 243–57. New Delhi: Springer India, 2016. http://dx.doi.org/10.1007/978-81-322-3640-5_15.

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Shiu, R., Y. Myal, D. Tsuyuki, D. Robinson, B. Iwasiow, A. Yarmill, and P. Watson. "The Prolactin-Inducible Protein / Gross Cystic Disease Fluid Protein (PIP/GCDFP-15): Genetic Analysis and Hormonal Regulation of Gene Expression." In Breast Cancer: Biological and Clinical Progress, 93–101. Boston, MA: Springer US, 1992. http://dx.doi.org/10.1007/978-1-4615-3494-5_7.

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Palkovits, Roland, Christian Mayer, and Thomas G. M. Schalkhammer. "Analysis in Complex Biological Fluids." In Analytical Biotechnology, 300–322. Basel: Birkhäuser Basel, 2002. http://dx.doi.org/10.1007/978-3-0348-8101-2_9.

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Dagnino, Sonia. "Analysis of PFASs in Biological Tissues and Fluids." In Toxicological Effects of Perfluoroalkyl and Polyfluoroalkyl Substances, 23–49. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-15518-0_2.

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Hansen, Steen Honoré, and Stig Pedersen-Bjergaard. "Analysis of Small-Molecule Drugs in Biological Fluids." In Bioanalysis of Pharmaceuticals, 207–60. Chichester, UK: John Wiley & Sons, Ltd, 2015. http://dx.doi.org/10.1002/9781118716830.ch9.

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Rakhit, A. "Analysis of Angiotensin-Converting Enzyme Inhibitors in Biological Fluids." In Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular, 135–42. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4757-9424-3_16.

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Reubsaet, Leon, and Trine Grønhaug Halvorsen. "Analysis of Peptide and Protein Drugs in Biological Fluids." In Bioanalysis of Pharmaceuticals, 261–82. Chichester, UK: John Wiley & Sons, Ltd, 2015. http://dx.doi.org/10.1002/9781118716830.ch10.

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Conference papers on the topic "Biological fluid analysis"

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Gao, Jianbo, Jing Hu, and Wen-wen Tung. "Multiscale Analysis of Biological Signals." In ASME 2011 Dynamic Systems and Control Conference and Bath/ASME Symposium on Fluid Power and Motion Control. ASMEDC, 2011. http://dx.doi.org/10.1115/dscc2011-6084.

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Complex systems often generate highly nonstationary and multiscale signals, due to nonlinear and stochastic interactions among their component systems and hierarchical regulations imposed by the operating environments. The further advances in the fields of life sciences, systems biology, nano-sciences, information systems, and physical sciences, have made it increasingly important to develop complexity measures that incorporate the concept of scale explicitly, so that different behaviors of the signals on varying scales can be simultaneously characterized by the same scale-dependent measure. Here, we propose such a measure, the scale-dependent Lyapunov exponent (SDLE), and develop a unified theory of multiscale analysis of complex data. We show that the SDLE can readily characterize low-dimensional chaos and random 1/fα processes, as well as accurately detect epileptic seizures from EEG data and distinguish healthy subjects from patients with congestive heart failure from heart rate variability (HRV) data. More importantly, our analyses of EEG and HRV data illustrate that commonly used complexity measures from information theory, chaos theory, and random fractal theory can be related to the values of the SDLE at specific scales, and useful information on the structured components of the data is also embodied by the SDLE.
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Lubman, David M., Chung Hang Sin, and Ho Ming Pang. "Analytical Applications Of Supercritical Fluid/Supersonic Beam Laser Ionization Mass Spectrometry." In Laser Applications to Chemical Analysis. Washington, D.C.: Optica Publishing Group, 1987. http://dx.doi.org/10.1364/laca.1987.tua4.

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Supercritical fluids of CO2 and N2O are used as a means of solubilizing nonvolatile polynuclear aromatic hydrocarbons and small thermally labile biologicals for expansion into supersonic beams for mass spectrometry. The resulting expansion into vacuum results in internally ultracold molecules with sharp spectral features for unique identification in UV-VIS absorption spectroscopy. In this work laser resonant two-photon ionization is used as a means of selectively producing ions for detection in mass spectrometry where the first photon excites the molecule to a real resonant state and the second photon ionizes the molecule. Although ions are produced for mass spectrometry, the ionization cross section reflects the SO → S1 transition so that ionization spectroscopy can be used as a means of identifying molecules in a mass spectrometer. We have applied this method to detection of several PNAH's where supercritical fluid expansion was used to solubilize these molecules essentially at room temperature, i.e. without heating. In addition, small biological and pharmaceutical analogs have been examined and the effect of the supercritical fluid solvent is studied. Most recently supercritical ammonia has been investigated as a highly polar solvent for dissolving indoleamines, catecholamines and other polar biological species. The key to this experiment is a novel compact molecular beam apparatus for supercritical fluid injection which takes advantage of (a) efficient liquid N2 cryopumping of CO2 and (b) a special high-pressure pulsed valve. This valve is capable of operation up to 400 atm backpressure at 250°C. The combination of pulsed valve injection with cryopumping allows the use of a 150-200 μ orifice for high on-axis density and thus sensitivity. The resulting chamber pressure at 400 atm reservoir pressure is <2×10−5 torr so that the ions produced by R2PI can be detected and mass analyzed in a time-of-flight mass spectrometer. The future potential of this method is discussed and compared to the use of the laser desorption method for volatilizing labile compounds into jet expansions.
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Gajjar, Jitesh S. B. "Preface of the "Symposium on recent advances in theoretical fluid dynamics, hydrodynamic stability theory, and biological fluid mechanics"." In 11TH INTERNATIONAL CONFERENCE OF NUMERICAL ANALYSIS AND APPLIED MATHEMATICS 2013: ICNAAM 2013. AIP, 2013. http://dx.doi.org/10.1063/1.4825470.

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Cimrák, Ivan. "Preface of the “Symposium on modelling of biological cells, fluid flow and microfluidics”." In PROCEEDINGS OF THE INTERNATIONAL CONFERENCE ON NUMERICAL ANALYSIS AND APPLIED MATHEMATICS 2014 (ICNAAM-2014). AIP Publishing LLC, 2015. http://dx.doi.org/10.1063/1.4912486.

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Menon, Prahlad G., William Kowalski, and Kerem Pekkan. "Computational Fluid Dynamics Analysis of Early Embryonic Aortic Arch-Ligation." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14470.

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Congenital heart disease occurs in 8 out of every 1000 live births in the US and more than half of this population is associated with great artery lesions. Selective remodeling of the paired, bilaterally symmetric embryonic aortic arches (AA) is a crucial stage in vascular morphogenesis and has known association with biomechanical forces [1]. Fetal cardiac interventions are currently explored clinically as an alternative repair technique for congenital anomalies, in-utero [2]. Several computational fluid dynamics (CFD) studies have been performed focusing on subject specific embryonic cardiovascular anatomies [3–5]. These developments could benefit fetal interventions that are planned in-silico before execution. To demonstrate this possibility, we computed the hemodynamic variation and wall shear stress (WSS) patterns resulting from systematic in-silico AA ligation intervention performed on normal chick AA models viz. Hamburger Hamilton (HH) stage 18 and 24 (3 and 4 days, respectively). A unique methodology employing CFD-computed WSS for modeling short-term biological growth response on AA morphogenesis is also presented.
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Schultz, Joshua A., and Jun Ueda. "Analysis of Antagonist Stiffness for Nested Compliant Mechanisms in Agonist-Antagonist Arrangements." In ASME 2011 Dynamic Systems and Control Conference and Bath/ASME Symposium on Fluid Power and Motion Control. ASMEDC, 2011. http://dx.doi.org/10.1115/dscc2011-5953.

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Members of the animal kingdom produce motion by muscle contraction. Biological muscle can be viewed as a unidirectional actuator. To achieve bidirectional motion, each muscle has a corresponding antagonist muscle whose contraction produces motion in the opposite direction. This gives biological systems the unique ability to modulate the stiffness of a joint, which is important when interacting with the environment. Certain bio-inspired robotic systems incorporate antagonistic pairs in an attempt to produce similar desirable properties. The cellular actuator employs nested compliant mechanisms to produce human-scale motion from piezoelectric stack actuators, which on their own have a small displacement. The expression for the stiffness of the actuator composed of these mechanisms takes the form of a continued fraction, which results from the nested structure. In this way, the stiffness can be easily approximated to a desired degree of accuracy by considering only the outermost mechanisms.
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Bel'skaya, L. "CORRELATION INTERCONNECTIONS OF BIOCHEMICAL COMPOSITION OF SALIVA AND CHARACTERISTICS OF INFRARED ABSORPTION SPECTRA." In XIV International Scientific Conference "System Analysis in Medicine". Far Eastern Scientific Center of Physiology and Pathology of Respiration, 2020. http://dx.doi.org/10.12737/conferencearticle_5fe01d9c216c81.02726336.

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The comparison of the characteristics of the infrared spectra (height, area of absorption bands) with the biochemical composition was carried out using the example of human saliva. Correlations of both individual absorption bands and their combinations with a number of biochemical parameters of saliva have been established. The substantiation of the revealed regularities based on the metabolic characteristics of this biological fluid is proposed.
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Almeida, Henrique A., and Paulo J. Ba´rtolo. "Computer Simulation and Optimisation of Tissue Engineering Scaffolds: Mechanical and Vascular Behaviour." In ASME 2008 9th Biennial Conference on Engineering Systems Design and Analysis. ASMEDC, 2008. http://dx.doi.org/10.1115/esda2008-59460.

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Additive biomanufacturing processes are increasingly recognised as ideal techniques to produce scaffolds for tissue engineering applications. These scaffolds must be biocompatible, biodegradable, with appropriate porosity, pore structure and pore distribution and optimal vascularisation, with both surface and structural compatibility. Surface compatibility means a chemical, biological and physical suitability to the host tissue. Structural compatibility corresponds to an optimal adaptation to the mechanical behaviour of the host tissue. Recent advances in tissue engineering field are increasingly relying on modelling and simulation. This paper proposes a novel computational tool combining structural, computational fluid dynamics and topological optimisation schemes, to predict and optimise both mechanical and vascular behaviour of scaffolds for soft and hard tissue applications, with different topological architectures and levels of porosity. This tool is particularly important to quantify the structural heterogeneity and scaffold mechanical properties with a designed microstructure subjected to either a single or a multiple load distribution. This computational tool enables the simulation of biological flows in vascular passages of scaffolds. The blood flow considered in this study is a complex fluid comprising a suspension of red blood cells, white blood cells and platelets within a newtonian plasma. A topological optimisation scheme is being developed to obtain the ideal scaffold topological architectures.
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Darabi, Jeff. "Numerical Analysis of Dielectrophoretic-Based DNA Separation and Trapping." In ASME 2022 Fluids Engineering Division Summer Meeting. American Society of Mechanical Engineers, 2022. http://dx.doi.org/10.1115/fedsm2022-87076.

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Abstract In this study, dielectrophoresis (DEP) has been coupled with field-flow fractionation (FFF) for the sorting and trapping of the biological particles. A numerical simulation is performed to compute particle trajectories under the influence of DEP, drag, gravitational, and buoyancy forces, as well as Brownian motion. The simulation was performed using OpenFOAM CFD software. Both positive and negative DEP methods are examined as possible separation techniques for DNA fragments. Positive DEP forces are used to attract the particles to the electrodes and trap them in groups of similar particles while a combination of negative DEP forces and field flow fractionation (FFF) are used to levitate the particles within the fluid flow to certain flow trajectories. The results obtained from this study, including electric field simulations, particle trajectories, elution times, and trapping lengths are presented.
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Gao, Yandong, Y. F. Yap, T. N. Wong, J. C. Chai, C. Yang, and K. T. Ooi. "Numerical Solution of Two-Fluid Electroosmotic Flow." In ASME 3rd International Conference on Microchannels and Minichannels. ASMEDC, 2005. http://dx.doi.org/10.1115/icmm2005-75005.

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Two-fluid flows in microchannel are often found in biological analysis, such as during ion exchange or solvent extraction from one phase to another. In this article, a numerical scheme is presented to describe a two-fluid flow in microchannel with electroosmotic (EO) effects. In this two-fluid system, the interfacial viscous force of a high EO mobility fluid drags a low EO mobility fluid; the high EO mobility fluid is driven by electroosmosis. We particularly analyze the electric double layer (EDL) regions close to the wall and the interface in the high EO mobility fluid. As the governing equation of the electrical potential is singularly perturbed, finer meshes are adopted to capture these EDL regions. In simulation, the interface between the two fluids evolves along the flow direction as the flow develops. Level set method is used to capture the interface implicitly. A localized mass preservation scheme is used to ensure mass conservation. A finite-volume method is used to solve the coupled electric potential equation, level set equations and Navier-Stokes equation. The validity of the numerical scheme is evaluated by comparing its predictions with the results of the analytical solutions in the fully developed regions. The interface positions; pressure gradients; mass flow rates and velocity profiles of the two fluids along the channels are obtained numerically.
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Reports on the topic "Biological fluid analysis"

1

Lin, Emil T., Leslie Z. Benet, Robert A. Upton, and Winnie L. Gee. Analysis of Investigational Drugs in Biological Fluids - Method Development and Routine Assay. Fort Belvoir, VA: Defense Technical Information Center, June 1991. http://dx.doi.org/10.21236/ada238981.

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Lin, Emil T. Analysis of Investigational Drugs in Biological Fluids - Method Development and Analysis of Pre-Clinical Samples. Fort Belvoir, VA: Defense Technical Information Center, September 2001. http://dx.doi.org/10.21236/ada399915.

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Lin, Emil T. Analysis of Investigational Drugs in Biological Fluids - Method Development and Analysis of Pre-Clinical and Clinical Samples. Fort Belvoir, VA: Defense Technical Information Center, September 2000. http://dx.doi.org/10.21236/ada391522.

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