Journal articles: 'Bioguided chemical study'

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Schmitt, Marie, Abdulmagid Alabdul Magid, Jean-Marc Nuzillard, Jane Hubert, Nicolas Etique, Laurent Duca, and Laurence Voutquenne-Nazabadioko. "Investigation of Antioxidant and Elastase Inhibitory Activities of Geum urbanum Aerial Parts, Chemical Characterization of Extracts Guided by Chemical and Biological Assays." Natural Product Communications 15, no. 3 (March 1, 2020): 1934578X2091530. http://dx.doi.org/10.1177/1934578x20915307.

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This study presents the bioguided chemical investigation of the 80% aqueous methanol extract of Geum urbanum aerial parts. Liquid–liquid partitioning of this extract in solvents of increasing polarity combined with biological screening showed that the ethyl acetate (EtOAc) soluble fraction was the most active part of the extract. This fraction was chemically profiled by a 13C nuclear magnetic resonance (NMR)-based dereplication method, resulting in the identification of 14 compounds. The dereplication process was followed by the purification of unknown and minor compounds of the EtOAc fraction. A new glycosylated phenol, namely, 3-(3,4-dihydroxyphenyl)propyl-α-l-rhamnopyranoside, together with 6 known compounds were isolated. Their structures were elucidated by spectroscopic methods including NMR and high-resolution electrospray ionization mass spectrometry. The antioxidant activity of fractions and isolated compounds were evaluated by 2,2,1-diphenyl-1-picrylhydrazyl and hydroxyl radical scavenging, and by cupric ion reducing antioxidant capacity assays. In parallel, their enzyme inhibitory property against human neutrophil elastase was assessed. Four subfractions, essentially containing polyphenols and triterpenes, exhibited a significant elastase inhibitory activity and an ellagitannin showed a very high radical scavenging activity.

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Hamion, Guillaume, Willy Aucher, Charles Tardif, Julie Miranda, Caroline Rouger, Christine Imbert, and Marion Girardot. "Valorization of Invasive Plant Extracts against the Bispecies Biofilm Staphylococcus aureus–Candida albicans by a Bioguided Molecular Networking Screening." Antibiotics 11, no. 11 (November 11, 2022): 1595. http://dx.doi.org/10.3390/antibiotics11111595.

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Invasive plants efficiently colonize non-native territories, suggesting a great production of bioactive metabolites which could be effective antibiofilm weapons. Our study aimed to look for original molecules able to inhibit bispecies biofilm formed by S. aureus and C. albicans. Extracts from five invasive macrophytes (Ludwigia peploides, Ludwigia grandiflora, Myriophyllum aquaticum, Lagarosiphon major and Egeria densa) were prepared and tested in vitro against 24 h old bispecies biofilms using a crystal violet staining (CVS) assay. The activities of the extracts reducing the biofilm total biomass by 50% or more were comparatively analyzed against each microbial species forming the biofilm by flow cytometry (FCM) and scanning electron microscopy. Extracts active against both species were fractionated. Obtained fractions were analyzed by UHPLC-MS/MS and evaluated by the CVS assay. Chemical and biological data were combined into a bioactivity-based molecular networking (BBMN) to identify active compounds. The aerial stem extract of L. grandiflora showed the highest antibiofilm activity (>50% inhibition at 50 µg∙mL−1). The biological, chemical and BBMN investigations of its fractions highlighted nine ions correlated with the antibiofilm activity. The most correlated compound, identified as betulinic acid (BA), inhibited bispecies biofilms regardless of the three tested couples of strains (ATCC strains: >40% inhibition, clinical isolates: ≈27% inhibition), confirming its antibiofilm interest.

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Messaoudi, Radja, Abdelkrim Cheriti, and Younes Bourmita. "BIOASSAY-GUIDED ISOLATION OF THE MAJOR COMPOUND WITH ANTIOXIDANT ACTIVITY FROM THE ALGERIAN MEDICINAL PLANT BUBONIUM GRAVEOLENS." Asian Journal of Pharmaceutical and Clinical Research 11, no. 11 (November 7, 2018): 424. http://dx.doi.org/10.22159/ajpcr.2018.v11i11.28514.

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Objective: The objective of this research was to isolate and identify the major compound from the antioxidant extract obtained from the leaves of Bubonium graveolens.Methods: A bioguided fractionation was done to isolate and identify the major compound responsible of the antioxidant activity. The chemical structure of the isolated natural compound was established by spectroscopic means including UV, Fourier transform infrared, 1H NMR, and13C NMR. The purified compound was accurately tested for its antioxidant activity by the 2, 2-diphenyl-1-picrylhydrazyl assay at 517 nm.Results: The major compound named myricetin 3′-O-rhamnoside was isolated for the 1st time from the leaves of B. graveolens a medicinal specie of Algerian Sahara belonging to the Asteraceae family. The study showed that the myricetin 3ʹ-O-rhamnoside has an antioxidant potential 59% when compared with standard ascorbic acid with an IC 50=0.916 mg/ml.Conclusion: In the present work, it was possible to isolate and identify for the 1st time the major compound from the antioxidant fraction of B. graveolens.

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Joseph, Rhodin C., Matheus Silva da Fonseca Diniz, Viviane Magno do Nascimento, Abraão de Jesus Barbosa Muribeca, Johan Carlos Costa Santiago, Luziane da Cunha Borges, Paulo Roberto da Costa Sá, et al. "Secure and Sustainable Sourcing of Plant Tissues for the Exhaustive Exploration of Their Chemodiversity." Molecules 25, no. 24 (December 18, 2020): 5992. http://dx.doi.org/10.3390/molecules25245992.

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The main challenge of plant chemical diversity exploration is how to develop tools to study exhaustively plant tissues. Their sustainable sourcing is a limitation as bioguided strategies and dereplication need quite large amounts of plant material. We examine if alternative solutions could overcome these difficulties by obtaining a secure, sustainable, and scalable source of tissues able to biosynthesize an array of metabolites. As this approach would be as independent of the botanical origin as possible, we chose eight plant species from different families. We applied a four steps culture establishment procedure, monitoring targeted compounds through mass spectrometry-based analytical methods. We also characterized the capacities of leaf explants in culture to produce diverse secondary metabolites. In vitro cultures were successfully established for six species with leaf explants still producing a diversity of compounds after the culture establishment procedure. Furthermore, explants from leaves of axenic plantlets were also analyzed. The detection of marker compounds was confirmed after six days in culture for all tested species. Our results show that the first stage of this approach aiming at easing exploration of plant chemodiversity was completed, and leaf tissues could offer an interesting alternative providing a constant source of natural compounds.

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Ismail, Amel, Leila Ktari, Yosr Ben Redjem Romdhane, Brahim Aoun, Saloua Sadok, Abdellatif Boudabous, and Monia El Bour. "Antimicrobial Fatty Acids from Green AlgaUlva rigida(Chlorophyta)." BioMed Research International 2018 (November 13, 2018): 1–12. http://dx.doi.org/10.1155/2018/3069595.

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This study deals with the antimicrobial potential assessment ofUlva rigida,in regard to collection period and sampling site. Besides, we assess the chemical composition of bioactive compounds. For this purpose,Ulva rigidawas seasonally collected from two northern sites in Tunisia, Cap Zebib rocky shore (CZ) and Ghar El Melh lagoon (GEM). Crude organic extracts were prepared using dichloromethane and dichloromethane/methanol and tested against 19 indicator microorganisms using the disk diffusion method and microdilution technique to determine the minimum inhibitory concentration (MIC). Silica gel column and thin layer chromatography were used for purification of active compounds. Nuclear magnetic resonance (NMR) and gas chromatography were used for compounds identification. Samples ofUlva rigidacollected from the two sites have uniform antimicrobial activity throughout the year. Algae collected from the lagoon showed the largest spectrum of activity and were used for subsequent analysis. Bioguided purification of extracts fromUlva rigida,collected at GEM, leads to 16 active fractions with antibacterial effect mainly againstStaphylococcus aureusATCC 25923 andEnterococcus faecalisATCC 29212. These fractions were identified as fatty acids, mainly oleic (C18: 1 w9), linoleic (C18: 2 w6), palmitic (C16: 0), and stearic (C14: 0). MICs values ranged from 10 to 250μg/ml.

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Ticona, Luis Apaza, Andreea Madalina Serban, Daly Apaza Ticona, and Karla Slowing. "Anti-inflammatory and Anti-arthritic Activities of Aqueous Extract and Flavonoids from Tripodanthus acutifolius Leaves in Mice Paw Oedema." Planta Medica International Open 8, no. 02 (May 27, 2021): e43-e55. http://dx.doi.org/10.1055/a-1471-8947.

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Abstract Tripodanthus acutifolius, commonly known as Jamillo, is an herbal remedy used in traditional Andean medicine to treat joint problems, such as sprains, dislocations, and rheumatic pain. This study aimed to evaluate the in vitro and in vivo anti-inflammatory and anti-arthritic activity of the aqueous extract and isolated compounds of T. acutifolius. A bioguided phytochemical analysis based on NMR/MS was performed to identify the compounds of the aqueous extract from T. acutifolius. The anti-inflammatory and anti-arthritic activity were evaluated by measuring inflammatory parameters (TNF-α, C-reactive protein, and fibrinogen) in murine models. The chemical structure determination led to the identification of four flavonoids: (E)-2’,4’-dihydroxy-6’-methoxy-chalcone (1), 6,2’,4’-trimethoxyflavone (2), 5,3’,4’-trihydroxy-6,7,8-trimethoxyflavone (3), and 5,4’-dihydroxy-6,7,8-trimethoxyflavone (4). All compounds inhibited the production of TNF-α in the RAW 264.7 cell line, with IC50 values of 0.78, 1.43, 5.73, and 9.71 μM, respectively. In addition, all flavonoids decreased the levels of TNF-α, C-reactive protein, and fibrinogen at a concentration of 5 mg/kg in murine models. Our research shows that these compounds isolated from T. acutifolius have anti-inflammatory and anti-arthritic properties, providing scientific evidence for the traditional use of this plant species.

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Fechine, Ivana Maria, Karolayne da Silva Barbosa Alves, Renam Fellipe da Silveira Muniz, Caroline Leal Rodrigues Soares, Harley da Silva Alves, Teresinha Gonçalves da Silva, Carlos Arthur Gouveia Veloso, Micheline de Azevedo Lima, and Evelise Márcia Locatelli de Souza. "Evaluation of the cytotoxicity of products obtained from Calotropis procera (Apocynaceae)." Research, Society and Development 9, no. 12 (December 13, 2020): e4391210723. http://dx.doi.org/10.33448/rsd-v9i12.10723.

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Calotropis procera belongs to the Apocynaceae family and is found in the Northeast of Brazil. Several activities are attributed to this species, such as anti-inflammatory, antibacterial and anti-cancer activities. This research aimed to conduct a bioguided study of C. procera in order to isolate and identify triterpenes of the species and evaluate the cytotoxic activity of products of the plant species. The crude ethanolic extract (CEE) was obtained and partitioned. The hexanic phase was chromatographed giving the compound Cp-1, which was identified using spectroscopic techniques and thermal analysis. It was possible to identify Cp-1 as a triterpene called calotropenyl acetate, already reported in the literature. The cytotoxicity test was performed using the MTT technique and it was observed a good activity of CEE and hexanic phase against HL-60 and KS62 lines with an IC50 ranging between 26.8 ± 0.4 µg mL-1 and 47.0 ± 0.5 µg mL-1, besides no toxicity in normal lines, while Cp-1 showed no relevant activity against the tumor cells tested. The results indicated that the species contains in its chemical composition products with pharmacological interest, in addition to cytotoxic for leukemic cells, which can be further explored in complementary studies.

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Yemoa, Achille, Joachim Gbenou, Dissou Affolabi, Mansourou Moudachirou, André Bigot, Séverin Anagonou, Françoise Portaels, Anandi Martin, and Joëlle Quetin-Leclercq. "Beninese Medicinal Plants as a Source of Antimycobacterial Agents: Bioguided Fractionation andIn VitroActivity of Alkaloids Isolated fromHolarrhena floribundaUsed in Traditional Treatment of Buruli Ulcer." BioMed Research International 2015 (2015): 1–5. http://dx.doi.org/10.1155/2015/835767.

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Buruli ulcer (BU) imposes a serious economic burden on affected households and on health systems that are involved in diagnosing the disease and treating patients. Research is needed to find cost-effective therapies for this costly disease. Plants have always been an important source of new pharmacologically active molecules. Consequently we decided to undertake the study of plants used in traditional treatment of BU in Benin and investigate their antimycobacterial activity as well as their chemical composition. Extracts from forty-four (44) plant species were selected on account of reported traditional uses for the treatment of BU in Benin and were assayed for antimycobacterial activities. Crude hydroethanolic extract from aerial parts ofHolarrhena floribunda(G. Don) T. Durand and Schinz was found to have significant antimycobacterial activity againstM.ulcerans(MIC = 125 µg/mL). We describe here the identification of four steroidal alkaloids fromMycobacterium ulceransgrowth-inhibiting fractions of the alkaloidal extract of the aerial parts ofHolarrhena floribunda. Holadysamine was purified in sufficient amount to allow the determination of its MCI (=50 µg/mL). These results give some support to the use of this plant in traditional medicine.

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Lima, Sandrine Maria Arruda, Pedro Silvino Pereira, Bruno Iraquitan Miranda da Silva, Natalie Emanuelle Ribeiro, Elizabeth Fernanda de Oliveira Borba, Cícera Datiane de Morais Oliveira Tintino, Karina Perrelli Randau, et al. "Antioxidant, antimicrobial and cytotoxic activities of secondary metabolites from Streptomyces sp. isolated of the Amazon-Brazil region." Research, Society and Development 10, no. 10 (August 14, 2021): e366101018974. http://dx.doi.org/10.33448/rsd-v10i10.18974.

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Bacteria of Streptomyces genus are a promising source of biologically active products, with applications in medicine, industry and agriculture. Therefore, the objective of this work was to evaluate the cytotoxic, antioxidant and antimicrobial activities of fermented rice extract and their semipurified fractions from Streptomyces spp. isolated of the rhizosphere of Paullinia cupana, Amazon-Brazil. For this, a bioguided study was carried out by the cytotoxic activity with methanolic extract of Streptomyces sp. ACTMS-12H UFPEDA 3405 (EMeOH-12H) partitioned with n-hexane, ethyl acetate and 2-butanol. The antioxidant activity was analyzed using the DPPH, ABTS and phosphomolybdenum methods, while the antimicrobial activity was investigated by microdilution method to determine the minimum inhibitory concentration (MIC) against species of bacteria and yeast. In the cytotoxicity test, the butanolic phase (FbuOH-12H) presented IC50 of 1.1 µg/mL against MOLT-4, with cell death probably by apoptosis, but did not cause cytotoxicity on peripheral blood mononuclear cell (PBMC) or human erythrocytes. Chemical prospecting detected the presence of saponins and reducing sugars on 2-butanol fraction (FBuOH-12H), which can be related to cytotoxicity. On the antioxidant activity by ABTS, the partition with ethyl acetate (FAcOEt-12H) showed antioxidant capacity of 1161.7 ± 0.04 µM of Trolox/g of extract, indicating an expressive reactivity of the phase with this radical. The aqueous phases (from hexane, ethyl acetate and methanol extracts) were active in all tested microorganisms, except E. faecalis.

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FRAUSIN, G., R. B. S. LIMA, A. F. HIDALGO, L. C. MING, and A. M. POHLIT. "Plants of the Araceae family for malaria and related diseases: a review." Revista Brasileira de Plantas Medicinais 17, no. 4 (December 2015): 657–66. http://dx.doi.org/10.1590/1983-084x/14_024.

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ABSTRACTIn the current work we performed a review of the Araceae family species traditionally used to treat malaria and its symptoms. The aim is to reveal the large number of antimalarial Araceae species used worldwide and their great unexplored potential as sources of antimalarial natural products. The SciFinder Scholar, Scielo, PubMed, ScienceDirect and Google books search engines were consulted. Forty-three records of 36 species and 23 genera of Araceae used for malaria and symptoms treatment were found. The neotropical genera Philodendron Schott and Anthurium Schott were the best represented for the use in the treatment of malaria, fevers, liver problems and headaches. Leaves and tubers were the most used parts and decoction was the most common preparation method. The extracts of Araceae species inhibit the in vitro growth of the human malaria parasite, the Plasmodium falciparum Welch, and significant median inhibitory concentrations (IC50) for extracts of guaimbê-sulcado (Rhaphidophora decursiva (Roxb.) Schott), aninga (Montrichardia linifera (Arruda) Schott), Culcasia lancifolia N.E. Br. and forest anchomanes (Anchomanes difformis (Blume) Engl.) have been reported demonstrating the antimalarial and cytotoxicity potential of the extracts and sub-fractions. In the only report about the antimalarial components of this family, the neolignan polysyphorin and the benzoperoxide rhaphidecurperoxin presented strong in vitro inhibition of the D6 and W2 strains of Plasmodiumfalciparum (IC50 = 368-540 ng/mL). No live study about antimalarial activity in animal models has been conducted on a species of Araceae. More bioguided chemical composition studies about the in vitro and also thein vivo antimalarial activity of the Araceae are needed in order to enhance the knowledge about the antimalarial potential of this family.

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Jonesusbel, Rainimanantsoa, Tiandreniny Jipaty, Fiatoa Barthelemy, Herindrainy Audiat Miller, Andrianjara Charles, and Fatiany Pierre Ruphin. "Antihypertenseur, Activité Vasorelaxant De Rouppelinia Boivinni (Apocynaceae) Et Etude De Relation Structure-Activité Des Alcaloïdes Indoliques Isolées." International Journal of Progressive Sciences and Technologies 41, no. 2 (November 14, 2023): 221. http://dx.doi.org/10.52155/ijpsat.v41.2.5688.

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In the southern and south-western regions of Madagascar, traditional medicine plays an important role in society. Ethnobotanical surveys carried out in these regions have revealed that the plant known under the vernacular name "Feka (nom Mahafaly, Masikoro); Fekambato (Nom Antandroy); Fekakely (Nom Sakalava)" is used by the local population to treat hypertension. The results of biological tests (in-vivo and in-vitro) carried out on the alkaloid extract show improved vasorelaxant activity. These results justify their therapeutic virtues. Bioguided fractionation of the alkaloid extract from the aerial part of this plant resulted in the isolation of two pure products, PX-1 and PX-2. The chemical structures of these two pure products were isolated using spectral analysis methods. The ultraviolet spectrum of the PX-1 molecule recorded by LHPC-UV-DAD shows an absorption maximum at 240nm and 317nm, indicating the presence of an acylindole chromophore in PX-1. The peak at Tr= 2.9min corresponds to the molecular ion peak at m/z =355.20141 [M+H]+, corresponding to the empirical formula C21H26N2O3. Product PX-2 has a molecular weight of m/z = 352.17896 [M+H]+, corresponding to the molecular formula C21H24N2O3, and its UV spectrum recorded by HPLC-UV-DAD shows absorption maxima at 240nm and 317nm consistent with the 2-acyl indole chromophore. Analysis by nuclear magnetic resonance spectroscopy (1D and 2D NMR) confirms that the basic structures of PX-1 and PX-2 are attributed to indole alkaloids. Analysis of the pharmacological mechanism of this plant's vasorelaxant activity shows that the relaxant activity may result from blockade of calcium influx through vascular smooth muscle cells, as the contraction produced by CaCl2 on the aorta in a calcium-free depolarizing medium was inhibited by the alkaloid extract in a concentration-dependent manner with EC50= 0.061±0.0056mg/ml at n=7. Preliminary acute and chronic toxicity studies carried out on the alkaloid extract of this plant showed no significant toxic signs. In perspective, the study of the plant used in medicine to treat TAH enables the isolation of two indole-type alkaloids and the discovery of their mechanisms of pharmacological activity. It's time for researchers to turn to traditional medicine as an inexhaustible source of scientific discovery.

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Álvarez Valverde, Víctor, Gerardo Rodríguez Rodríguez, and Silvia Argüello Vargas. "Bioguided Phytochemical Study of Ipomoea cairica Extracts with Larvicidal Activity against Aedes aegypti." Molecules 27, no. 4 (February 16, 2022): 1348. http://dx.doi.org/10.3390/molecules27041348.

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Vector-borne diseases, such as those transmitted by Aedes aegypti, are a constant threat to inhabitants of tropical regions of the planet. Synthetic chemicals are commonly used as a strategy to control them; however, these products are known to persist in ecosystems and drive the appearance of resistance genes in arthropod vectors. Thus, the use of natural products has emerged as an environmentally friendly alternative in integrated vector control strategies. The present bioguided study investigated the larvicidal potential of Ipomoea cairica extracts, fractionated using thin-layer and open-column chromatography, because this species has been shown to exert larvicidal effects on the genus Aedes. The objective of this study was to evaluate the nonvolatile components in ethanolic extract of I. cairica stems as a potential natural larvicidal, and coumarins, such as 7-hydroxy-6-methoxychromen-2-one (scopoletin) and 7-hydroxychromen-2-one (umbelliferone), were identified as major compounds; however, they were not shown to be responsible for the larvicidal activity. Based on the results of the larvicidal action tests, these coumarins are not directly responsible for the larvicidal activity, but this activity might be attributed to a synergistic effect of all the compounds present in the most active secondary fraction, called F.DCM, which had an LC50 value of 30.608 mg/L. This type of study has yet not been conducted in the region; therefore, it is an important contribution to recognizing a natural and easy-to-cultivate source of vector control, such I. cairica.

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Tegasne, Catherine, Gilbert Deccaux Wabo Fotso Kapche, Isabelle Kamga Mawabo, Rostan Mangoua Talla, Jean-Bosco Jouda, Gervais Mouthé Happi, Bruno Ndjakou Lenta, Marcel Frese, Berhanu Molah Abegaz, and Norbert Sewald. "Bioguided chemical study of Boswellia dalzielii Hutch. (Burseraceae) for antibacterial agents and a new glucopyranoxylmethoxybenzyle." Natural Product Research, July 30, 2020, 1–10. http://dx.doi.org/10.1080/14786419.2020.1794863.

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Khongkarat, Phanthiwa, Rico Ramadhan, Preecha Phuwapraisirisan, and Chanpen Chanchao. "Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities." Journal of Apicultural Science, March 3, 2021. http://dx.doi.org/10.2478/jas-2021-0001.

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Abstract Bee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species, plant source and biogeography. For this study, bee pollen was collected from Apis mellifera, harvested from the flowers of mimosa (Mimosa pigra L.) in the Chiang Mai province, Thailand. The sample was extracted in methanol (MeOH) and then sequentially partitioned with hexane, dichloromethane (DCM) and MeOH in order to isolate compounds depending on their polarities. The obtained extracts were then tested for their antioxidant and anti-tyrosinase activities through 1,1-diphyenyl-2-picrylhydrazyl (DPPH) assay and for/through inhibition of mushroom tyrosinase extract, respectively. The DCM partitioned extract of mimosa flower bee pollen (DCMMBP) provided the highest antioxidant activity, with an effective concentration at 50% (EC50) of 192.1 μg/mL, and was further fractionated by silica gel 60 column chromatography and Sephadex LH20 size exclusion chromatography. All fractions were tested for their antioxidant activity and analyzed for the chemical structure through nuclear magnetic resonance (NMR). The most active fraction (EC50 of 121.3 μg/mL) was a mixture of compounds, but the isolated flavonoid, naringenin, had a negligible antioxidant activity. In contrast, all three partitioned extracts and pooled fractions after silica gel 60 column chromatography provided no anti-tyrosinase activity (IC50 of > 500 μg/mL) and a very low percentage of tyrosinase inhibition (0–2.99 ± 3.18%), compared to kojic acid (IC50 of 8.58 μg/mL and tyrosinase inhibition of 74.2 ± 1.25%).

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Ticona, Luis Apaza, Ángel Rumbero Sánchez, Cristina Thiebaut Estrada, and Olga M. Palomino. "Identification of TRPV1 Ion Channels Agonists of Tropaeolum tuberosum in Human Skin Keratinocytes." Planta Medica, October 2, 2020. http://dx.doi.org/10.1055/a-1264-0572.

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Abstract Tropaeolum tuberosum, commonly known as Mashua, is an herbal remedy used in traditional Andean medicine for the relief of kidney and bladder pain, as well as contusions. This study aimed to evaluate the fractions and isolated compounds from T. tuberosum with analgesic activity mediated by the transient receptor potential vanilloid-1 receptor. A bioguided phytochemical analysis based on NMR/MS was performed to identify the compounds of the n-heptane fractions from samples of purple tubers of T. tuberosum. The transient receptor potential vanilloid-1 agonist and antagonist activity were assessed through the measurement of intracellular Ca2+ in HEK001 cells. The chemical structure determination led to the identification of two alkamides: N-(2-hydroxyethyl)-7Z,10Z,13Z,16Z-docosatetraenamide (1) and N-oleoyldopamine (2). Both compounds induced increased intracellular calcium flow with IC50 values of 3.2 nM and 7.9 nM, respectively, thus activating the transient receptor potential vanilloid-1 receptor. Our research is the first report to show that these two compounds isolated from T. tuberosum can act as agonists of the transient receptor potential vanilloid-1 receptor, providing scientific evidence for the traditional use of this species in pain relief.

Journal articles on the topic 'Bioguided chemical study'

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