Relevant bibliographies by topics / Biofilms – Research

Academic literature on the topic 'Biofilms – Research'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Biofilms – Research"

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Rittmann, B. E. "Where are we with biofilms now? Where are we going?" Water Science and Technology 55, no. 8-9 (April 1, 2007): 1–7. http://dx.doi.org/10.2166/wst.2007.235.

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The IWA's BiofilmVI conference presented a wide range of research on biofilm systems. Particularly popular themes were nitrogen removal, mathematical modelling and microbial ecology. Emerging themes included biofilms with membranes, pathogens in biofilms, biofouling and detachment. Within microbial ecology and mathematical modelling, emphasis was given to N-removal systems, particularly involving nitrifiers and Anammox bacteria. Both themes also recognised the importance of biofilm detachment. Although biofilms on membranes gained attention, little interest was exhibited towards linking biofilms with other advanced materials, such as ceramics, conductors, semi-conductors or nano-materials. Research presented at BiofilmVI marked major advances in improving water sustainability towards removing BOD and N, but did not address many emerging contaminants, such as oxidised contaminants and endocrine disruptors. Attention to energy sustainability, such as with bio-hydrogen or microbial fuel cells, was minimal. Thus, research reported at BiofilmVI was strong towards “improving the expected” with regard to BOD and N removal, but not yet focused on “exploiting the unexpected” to deal with emerging pollutants and bio-energy.
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Jiang, Yu, Mengxin Geng, and Liping Bai. "Targeting Biofilms Therapy: Current Research Strategies and Development Hurdles." Microorganisms 8, no. 8 (August 11, 2020): 1222. http://dx.doi.org/10.3390/microorganisms8081222.

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Biofilms are aggregate of microorganisms in which cells are frequently embedded within a self-produced matrix of extracellular polymeric substance (EPS) and adhere to each other and/or to a surface. The development of biofilm affords pathogens significantly increased tolerances to antibiotics and antimicrobials. Up to 80% of human bacterial infections are biofilm-associated. Dispersal of biofilms can turn microbial cells into their more vulnerable planktonic phenotype and improve the therapeutic effect of antimicrobials. In this review, we focus on multiple therapeutic strategies that are currently being developed to target important structural and functional characteristics and drug resistance mechanisms of biofilms. We thoroughly discuss the current biofilm targeting strategies from four major aspects—targeting EPS, dispersal molecules, targeting quorum sensing, and targeting dormant cells. We explain each aspect with examples and discuss the main hurdles in the development of biofilm dispersal agents in order to provide a rationale for multi-targeted therapy strategies that target the complicated biofilms. Biofilm dispersal is a promising research direction to treat biofilm-associated infections in the future, and more in vivo experiments should be performed to ensure the efficacy of these therapeutic agents before being used in clinic.
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Keir, J., L. Pedelty, and A. C. Swift. "Biofilms in chronic rhinosinusitis: systematic review and suggestions for future research." Journal of Laryngology & Otology 125, no. 4 (February 11, 2011): 331–37. http://dx.doi.org/10.1017/s0022215111000016.

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AbstractBackground:A biofilm is a community of micro-organisms encased within a self-produced, extracellular, polymeric substance. The role of biofilms as a major pathological aetiology in chronic rhinosinusitis would help explain the clinical manifestation of the disease.Objectives:To examine the current evidence, and to discuss possible future research directions, in relation to biofilms and chronic rhinosinusitis.Study design:Systematic literature review.Evaluation method:Two assessors independently undertook critical appraisal of the studies identified by the literature search. Significant findings were incorporated into this review. The primary outcome assessed was the presence of biofilm in human mucosal biopsy samples taken from patients with chronic rhinosinusitis, and from healthy controls.Results:We identified 11 studies examining biofilm formation in human mucosal biopsy samples taken from patients with chronic rhinosinusitis.Conclusion:It is unlikely that biofilms occur in every case of chronic rhinosinusitis; consequently, the significance of ‘biofilm detection’ in some series should be considered carefully. Several authors have argued strongly for the use of confocal scanning laser microscopy with fluorescent in situ hybridisation probes as the ‘gold standard’ for biofilm imaging. This imaging modality should be combined with further investigation of the microbiology of chronic rhinosinusitis, and of the efficacy of traditional culture techniques used for pathogen identification.
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Rocco, Christopher J., Lauren O. Bakaletz, and Steven D. Goodman. "Targeting the HUβ Protein PreventsPorphyromonas gingivalisfrom Entering into Preexisting Biofilms." Journal of Bacteriology 200, no. 11 (February 5, 2018): e00790-17. http://dx.doi.org/10.1128/jb.00790-17.

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ABSTRACTThe oral cavity is home to a wide variety of bacterial species, both commensal, such as various streptococcal species, and pathogenic, such asPorphyromonas gingivalis, one of the main etiological agents of periodontal disease. Our understanding of how these bacteria ultimately cause disease is highly dependent upon understanding how they coexist and interact with one another in biofilm communities and the mechanisms by which biofilms are formed. Our research has demonstrated that the DNABII family of DNA-binding proteins are important components of the extracellular DNA (eDNA)-dependent matrix of bacterial biofilms and that sequestering these proteins via protein-specific antibodies results in the collapse of the biofilm structure and release of the resident bacteria. While the high degree of similarity among the DNABII family of proteins has allowed antibodies derived against specific DNABII proteins to disrupt biofilms formed by a wide range of bacterial pathogens, the DNABII proteins ofP. gingivalishave proven to be antigenically distinct, allowing us to determine if we can use anti-P. gingivalisHUβ antibodies to specifically target this species for removal from a mixed-species biofilm. Importantly, despite forming homotypic biofilmsin vitro,P. gingivalismust enter preexisting biofilmsin vivoin order to persist within the oral cavity. The data presented here indicate that antibodies derived against theP. gingivalisDNABII protein, HUβ, reduce by half the amount ofP. gingivalisorganisms entering into preexisting biofilm formed by four oral streptococcal species. These results support our efforts to develop methods for preventing and treating periodontal disease.IMPORTANCEPeriodontitis is one of the most prevalent chronic infections, affecting 40 to 50% of the population of the United States. The root cause of periodontitis is the presence of bacterial biofilms within the gingival space, withPorphyromonas gingivalisbeing strongly associated with the development of the disease. Periodontitis also increases the risk of secondary conditions and infections such as atherosclerosis and infective endocarditis caused by oral streptococci. To induce periodontitis,P. gingivalisneeds to incorporate into preformed biofilms, with oral streptococci being important binding partners. Our research demonstrates that targeting DNABII proteins with an antibody disperses oral streptococcus biofilm and preventsP. gingivalisentry into oral streptococcus biofilm. These results suggest potential therapeutic treatments for endocarditis caused by streptococci as well as periodontitis.
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Ise, Kotaro, Tomofumi Sato, Yoshito Sasaki, and Hideki Yoshikawa. "Development of simplified biofilm sorption and diffusion experiment method using Bacillus sp. isolated from Horonobe Underground Research Laboratory." MRS Proceedings 1665 (2014): 171–77. http://dx.doi.org/10.1557/opl.2014.643.

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ABSTRACTWe developed a simplified biofilm sorption and diffusion experiment method. The biofilms of the Bacillus cereus were incubated on cellulose acetate membrane filters (pore size 0.2 µm, diameter 47 mm) placed on thick NB broth agar medium (thickness was about 30 mm) to support sufficient biofilm growth of the Bacillus cereus. The thickness of the formed biofilms was about 1 mm. The formed biofilms were applied to through-diffusion method, which has been used to measure diffusion coefficient of crystalline and sedimentary rocks and clay minerals. The obtained copper sorption coefficient by batch experiments was about 100 ml/g (wet weight) at the case of the concentration of cupper ion was over 0.074mmol/L. And diffusion coefficients by through diffusion experiment was De=1.1 x 10-10 (m2/s). From these results, this simplified biofilm sorption and diffusion experiment may make possible to obtain these parameters with ease.
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Yin, Wen, Yiting Wang, Lu Liu, and Jin He. "Biofilms: The Microbial “Protective Clothing” in Extreme Environments." International Journal of Molecular Sciences 20, no. 14 (July 12, 2019): 3423. http://dx.doi.org/10.3390/ijms20143423.

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Microbial biofilms are communities of aggregated microbial cells embedded in a self-produced matrix of extracellular polymeric substances (EPS). Biofilms are recalcitrant to extreme environments, and can protect microorganisms from ultraviolet (UV) radiation, extreme temperature, extreme pH, high salinity, high pressure, poor nutrients, antibiotics, etc., by acting as “protective clothing”. In recent years, research works on biofilms have been mainly focused on biofilm-associated infections and strategies for combating microbial biofilms. In this review, we focus instead on the contemporary perspectives of biofilm formation in extreme environments, and describe the fundamental roles of biofilm in protecting microbial exposure to extreme environmental stresses and the regulatory factors involved in biofilm formation. Understanding the mechanisms of biofilm formation in extreme environments is essential for the employment of beneficial microorganisms and prevention of harmful microorganisms.
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FOLSOM, JAMES P., and JOSEPH F. FRANK. "Chlorine Resistance of Listeria monocytogenes Biofilms and Relationship to Subtype, Cell Density, and Planktonic Cell Chlorine Resistance." Journal of Food Protection 69, no. 6 (June 1, 2006): 1292–96. http://dx.doi.org/10.4315/0362-028x-69.6.1292.

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Strains of Listeria monocytogenes vary in their ability to produce biofilms. This research determined if cell density, planktonic chlorine resistance, or subtype are associated with the resistance of L. monocytogenes biofilms to chlorine. Thirteen strains of L. monocytogenes were selected for this research based on biofilm accumulation on stainless steel and rep-PCR subtyping. These strains were challenged with chlorine to determine the resistance of individual strains of L. monocytogenes. Planktonic cells were exposed to 20 to 80 ppm sodium hypochlorite in 20 ppm increments for 5 min in triplicate per replication, and the experiment was replicated three times. The number of tubes with surviving L. monocytogenes was recorded for each isolate at each level of chlorine. Biofilms of each strain were grown on stainless steel coupons. The biofilms were exposed 60 ppm of sodium hypochlorite. When in planktonic culture, four strains were able to survive exposure to 40 ppm of chlorine, whereas four strains were able to survive 80 ppm of chlorine in at least one of three tubes. The remaining five strains survived exposure to 60 ppm of chlorine. Biofilms of 11 strains survived exposure to 60 ppm of chlorine. No association of biofilm chlorine resistance and planktonic chlorine resistance was observed; however, biofilm chorine resistance was similar for strains of the same subtype. Biofilm cell density was not associated with chlorine resistance. In addition, biofilms that survived chlorine treatment exhibited different biofilm morphologies. These data suggest that chlorine resistance mechanisms of planktonic cells and biofilms differ, with planktonic chlorine resistance being more affected by inducible traits, and biofilm chlorine resistance being more affected by traits not determined in this study.
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Xu, Tao, Yue Xiao, Hongchao Wang, Jinlin Zhu, Yuankun Lee, Jianxin Zhao, Wenwei Lu, and Hao Zhang. "Characterization of Mixed-Species Biofilms Formed by Four Gut Microbiota." Microorganisms 10, no. 12 (November 25, 2022): 2332. http://dx.doi.org/10.3390/microorganisms10122332.

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In natural settings, approximately 40–80% of bacteria exist as biofilms, most of which are mixed-species biofilms. Previous studies have typically focused on single- or dual-species biofilms. To expand the field of study on gut biofilms, we found a group of gut microbiota that can form biofilms well in vitro: Bifidobacterium longum subsp. infantis, Enterococcus faecalis, Bacteroides ovatus, and Lactobacillus gasseri. The increase in biomass and bio-volume of the mixed-species biofilm was confirmed via crystal violet staining, field emission scanning electron microscopy, and confocal laser scanning microscopy, revealing a strong synergistic relationship in these communities, with B. longum being the key biofilm-contributing species. This interaction may be related to changes in the cell number, biofilm-related genes, and metabolic activities. After quantifying the cell number using quantitative polymerase chain reaction, B. longum and L. gasseri were found to be the dominant flora in the mixed-species biofilm. In addition, this study analyzed biological properties of mixed-species biofilms, such as antibiotic resistance, cell metabolic activity, and concentration of water-insoluble polysaccharides. Compared with single-species biofilms, mixed-species biofilms had higher metabolic activity, more extracellular matrix, and greater antibiotic resistance. From these results, we can see that the formation of biofilms is a self-protection mechanism of gut microbiota, and the formation of mixed-species biofilms can greatly improve the survival rate of different strains. Finally, this study is a preliminary exploration of the biological characteristics of gut biofilms, and the molecular mechanisms underlying the formation of biofilms warrant further research.
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van Loosdrecht, M. C. M., D. Eikelboom, A. Gjaltema, A. Mulder, L. Tijhuis, and J. J. Heijnen. "Biofilm structures." Water Science and Technology 32, no. 8 (October 1, 1995): 35–43. http://dx.doi.org/10.2166/wst.1995.0258.

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The influences of reactor conditions (substrate loading rate and shear) and microbial characteristics (yield and growth rate) on the structure of biofilms is discussed. Based on research on the formation of biofilms in Biofilm Airlift Suspension (BAS) reactors a hypothesis is postulated that the ratio between biofilm surface loading and shear rate determines the biofilm structure. When shear forces are relatively high only a patchy biofilm will develop, whereas at low shear rates the biofilm becomes highly heterogeneous with many pores and protuberances. In case of a right balance smooth and stable biofilms can be obtained. A hypothesis for the evolution of biofilm structures as a function of process conditions is formulated.
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Lewandowski, Z., H. Beyenal, and D. Stookey. "Reproducibility of biofilm processes and the meaning of steady state in biofilm reactors." Water Science and Technology 49, no. 11-12 (June 1, 2004): 359–64. http://dx.doi.org/10.2166/wst.2004.0880.

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The need for reproducing biofilm processes is undisputable - the quality of biofilm research depends on this reproducibility. However, as many biofilm researchers know, long-term biofilm processes are notoriously difficult to reproduce. To avoid problems related to biofilm reproducibility two strategies are used: (1) to study very young biofilms that have accumulated for a few hours to a few days only, and (2) to run biofilm experiments only once. The first approach trades reproducibility for relevance because natural biofilms are usually older, often much older than a few days. This approach can be applied to answer questions relevant to initial events of biofilm formation but not questions relevant to long-term biofilm accumulation. The second approach conceals the problem of biofilm reproducibility. To assure reproducibility of biofilm processes, we methodically followed a procedure for growing biofilms in terms of microbial makeup, media composition, temperature, surface preparation, etc. Despite all this effort the reproducibility of our results for long term growth is unimpressive. Consequently, the question had to be asked: Are biofilm processes reproducible? The experiments described in this paper address this question. Biofilms grown in two identical and identically operated biofilm reactors had comparable structure only until the first sloughing event. After that, biofilms had different patterns of accumulation.
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Dissertations / Theses on the topic "Biofilms – Research"

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Crawford, Robert William. "Chronic Carriage of Salmonella: Biofilms and Gallstones." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1243956689.

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Kurth, Jonah C. "Mitigating biofilm growth through the modification of concrete design and practice." Thesis, Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/22694.

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Thesis (M. S.)--Civil and Environmental Engineering, Georgia Institute of Technology, 2008.
Committee Chair: Kurtis, Kimberly; Committee Member: Kahn, Lawrence; Committee Member: Sobecky, Patricia.
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Menicucci, Joseph Anthony Jr. "Algal biofilms, microbial fuel cells, and implementation of state-of-the-art research into chemical and biological engineering laboratories." Thesis, Montana State University, 2010. http://etd.lib.montana.edu/etd/2010/menicucci/MenicucciJ0510.pdf.

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Alternative energy technologies become more attractive as the price of energy from fossil fuels becomes more expensive and the environmental concerns from their use mount. While a number of biological alternative energy technologies currently exist, a complete understanding of these technologies has yet to be developed. This dissertation characterizes an aspect of biological alternative energy technologies: the production of algal biofuels and energy conversion in microbial fuel cells. Specifically, this dissertation addresses the characterization of microalgae as a biofilm and the characterization of the power limitations of microbial fuel cells. The attachment and detachment of algae were observed using temporal microscopic imaging in a flow-cell with autofluorescence and staining techniques as part of a collaborative Montana State University and Idaho National Laboratory project. Colonies of algae exhibit many characteristics seen in bacterial biofilms: adherence; detachment and sloughing; difference in structure of an attached colony; varying strength of attachment on different surfaces; association of other organisms in an EPS matrix; and the heterogeneous nature of attached colonies. The characterization of a microbial fuel cell was completed in less than 30 minutes using an empirical procedure to predict the maximum sustainable power that can be generated by a microbial fuel cell over a short period of time. In this procedure, the external resistance was changed incrementally, in steps of 500 ohms every 60 seconds, and the anode potential, the cathode potential, and the cell current were measured. This procedure highlights the inherent limitations of energy conversion in a microbial fuel cell. A voltage/current characterization of the microbial fuel was also completed from the data collected. This dissertation also includes the evaluation of A Hands-On Introduction to Microbial Fuel Cells, a laboratory developed for an introductory chemical and biological engineering course. The experiment has been updated to include a voltage/current characterization of the microbial fuel cell. Learning objectives have been identified and pre- and post-laboratory activities have been developed for further implementation into a chemical and biological engineering curriculum.
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Mathias, Elizabeth. "Exopolysaccharides of the Pseudomonas aeruginosa Biofilm Matrix." Ohio University Honors Tutorial College / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1400069245.

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ALBOSLEMY, TALIB. "Kruppel-like factor 2: A regulator of macrophage-mediated innate immune response against Staphylococcus aureus biofilm." Kent State University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=kent1523544938448141.

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Peng, Chao. "Cationic Polyurethanes for Antimicrobial Applications." University of Akron / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=akron1542383983590224.

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Gupta, Tripti Thapa. "Characterization and Optimization of Non-thermal Plasma for Biofilm Sterilization." University of Toledo / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=toledo152547566313079.

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Almutairi, Layla Awad. "Effects of Mild Magnetic Nanoparticle Hyperthermia on the Susceptibility of Biofilm Infections to Antibiotics." Kent State University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=kent1586725182180555.

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Grose, William E. "Characterization of a genetic locus in Burkholderia pseudomallei encoding a putative biofilm-associated protein." University of Toledo Health Science Campus / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=mco1301585276.

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Robison, Pamula J. "Mathematical Modelling of Biofilm Growth and Decay Through Various Deliveries of Antimicrobial." University of Akron / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=akron1258769688.

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Books on the topic "Biofilms – Research"

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Williams, Dustin L., ed. Targeting Biofilms in Translational Research, Device Development, and Industrial Sectors. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-30667-0.

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Jakubovics, Nicholas S., and Palmer Robert J. Oral microbial ecology: Current research and new perspectives. Norfolk, UK: Caister Academic Press, 2013.

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British Biofilm Club. Meeting Gregynog Hall, Wales. Biofilms: The good, the bad and the ugly ; contributions made at the Fourth Meeting of the British Biofilm Club held at Gregynog Hall, Powys 18-20 September, 1999. Cardiff: BioLine for the British Biofilm Club, 1999.

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Lewandowski, Zbigniew. Fundamentals of biofilm research. Boca Raton, FL: CRC Press, 2007.

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Nag, Moupriya, and Dibyajit Lahiri, eds. Analytical Methodologies for Biofilm Research. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1378-8.

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Kuspradini, Harlinda. Investigation of biofilm inhibition by several medicinal and aromatic plants from East Kalimantan: Final report international collaborative research and international publication. Samarinda]: Mulawarman University, 2012.

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(Editor), Hilary M. Lappin-Scott, and J. William Costerton (Editor), eds. Microbial Biofilms (Biotechnology Research). Cambridge University Press, 2003.

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Fundamentals of Biofilm Research. CRC, 2007.

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Lewandowski, Zbigniew, and Haluk Beyenal. Fundamentals of Biofilm Research. Taylor & Francis Group, 2013.

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Lewandowski, Zbigniew, and Haluk Beyenal. Fundamentals of Biofilm Research. Taylor & Francis Group, 2007.

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Book chapters on the topic "Biofilms – Research"

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Aransiola, S. A., M. O. Victor-Ekwebelem, Olusegun Julius Oyedele, and Naga Raju Maddela. "Futurity of Microbial Biofilm Research." In Microbial Biofilms, 319–26. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003184942-19.

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Ashton, Nicholas N., and Dustin L. Williams. "Targeting Biofilms in Translational Research." In Targeting Biofilms in Translational Research, Device Development, and Industrial Sectors, 131–55. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-30667-0_9.

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Heuvel, J. C., D. Beer, and C. C. H. Cronenberg. "Microelectrodes: A Versatile Tool in Biofilm Research." In Biofilms — Science and Technology, 631–44. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-1824-8_56.

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Cooksey, K. E. "Practical Needs for Basic Research: A Basis for Interaction between Microbiologists and Engineers." In Biofilms — Science and Technology, 677–81. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-1824-8_60.

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Facal Marina, Paula, Laurine Kaul, Nils Mischer, and Katharina Richter. "Metal-Based Nanoparticles for Biofilm Treatment and Infection Control: From Basic Research to Clinical Translation." In Springer Series on Biofilms, 467–500. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-10992-8_18.

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Goswami, Karan, and Javad Parvizi. "Targeting Biofilms in Orthopedic Infection." In Targeting Biofilms in Translational Research, Device Development, and Industrial Sectors, 71–83. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-30667-0_5.

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Bergel, Alain. "Recent Advances in Electron Transfer Between Biofilms and Metals." In Advanced Materials Research, 329–34. Stafa: Trans Tech Publications Ltd., 2007. http://dx.doi.org/10.4028/0-87849-452-9.329.

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Drewniak, Lukasz, Aleksandra Styczek, and Aleksandra Sklodowska. "Arsenic Hypertolerant Bacteria Isolated from Gold Mine Rocks Biofilms." In Advanced Materials Research, 576. Stafa: Trans Tech Publications Ltd., 2007. http://dx.doi.org/10.4028/0-87849-452-9.576.

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Akers, Kevin S., Joseph C. Wenke, and Clinton K. Murray. "Biofilms and Wound Infection Research in the US Military." In Targeting Biofilms in Translational Research, Device Development, and Industrial Sectors, 55–69. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-30667-0_4.

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Hoyle, Brian D., and J. William Costerton. "Bacterial resistance to antibiotics: The role of biofilms." In Progress in Drug Research / Fortschritte der Arzneimittelforschung / Progrès des recherches pharmaceutiques, 91–105. Basel: Birkhäuser Basel, 1991. http://dx.doi.org/10.1007/978-3-0348-7139-6_2.

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Conference papers on the topic "Biofilms – Research"

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Arrieta, M. P., M. A. Peltzer, M. C. Garrigos, and A. Jiménez. "Antibacterial biofilms based on calcium caseinate incorporated with carvacrol." In MICROBES IN APPLIED RESEARCH - Current Advances and Challenges. WORLD SCIENTIFIC, 2012. http://dx.doi.org/10.1142/9789814405041_0095.

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Ünal, Emel, Selin Kalkan, and Zerrin Erginkaya. "Use of lactic acid bacteria biofilms as biocontrol agents." In Proceedings of the International Conference on Antimicrobial Research (ICAR2010). WORLD SCIENTIFIC, 2011. http://dx.doi.org/10.1142/9789814354868_0040.

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Natalia, Angel Villegas, Arce Miranda Julio Eduardo, Becerra María Cecilia, Baronetti José Luis, Ravetti Soledad, Sotomayor Claudia Elena, Albesa Inés, and Paraje María Gabriela. "How different culture conditions affect cellular stresses in the biofilms." In MICROBES IN APPLIED RESEARCH - Current Advances and Challenges. WORLD SCIENTIFIC, 2012. http://dx.doi.org/10.1142/9789814405041_0128.

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Tkachuk, Nataliia. "Biofilms of Some Anaerobic Bacteria on the Polyethylene Terephthalate Surface." In IBRAS 2021 INTERNATIONAL CONFERENCE ON BIOLOGICAL RESEARCH AND APPLIED SCIENCE. Juw, 2021. http://dx.doi.org/10.37962/ibras/2021/188-90.

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Humbert, F., S. Saadi, F. Quilès, and K. Hani. "In situ assessment of antibacterial activity of dermaseptine S4 derivatives against Pseudomonas fluorescens nascent biofilms by using ATR-FTIR spectroscopy." In MICROBES IN APPLIED RESEARCH - Current Advances and Challenges. WORLD SCIENTIFIC, 2012. http://dx.doi.org/10.1142/9789814405041_0111.

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Retnaningrum, Endah, and Wahyu Wilopo. "Performance and bacterial composition of anodic biofilms in microbial fuel cell using dairy wastewater." In TOWARDS THE SUSTAINABLE USE OF BIODIVERSITY IN A CHANGING ENVIRONMENT: FROM BASIC TO APPLIED RESEARCH: Proceeding of the 4th International Conference on Biological Science. Author(s), 2016. http://dx.doi.org/10.1063/1.4953492.

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Saeed, Ahmed Yas, Sabah Ahmed Naif Aljubori, Mahmood Khalaf Saleh, Mostafa Qahtan Mostafa, Nedaa Wasmi Shehab, and Abdullah Dhahir Abdullah Mohammed. "Adhesion and quantitative role of biofilms in multiple antibiotic resistance of Pseudomonas aeruginosa isolated from different clinical infections." In PROCEEDING OF THE 1ST INTERNATIONAL CONFERENCE ON ADVANCED RESEARCH IN PURE AND APPLIED SCIENCE (ICARPAS2021): Third Annual Conference of Al-Muthanna University/College of Science. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0095327.

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Chou, Fong-In, Chia-Chin Li, Tzung-Yuang Chen, and Hsiao-Wei Wen. "Microbial Occurrence in Bentonite-Based Buffer Materials of a Final Disposal Site for Low Level Radioactive Waste in Taiwan." In ASME 2010 13th International Conference on Environmental Remediation and Radioactive Waste Management. ASMEDC, 2010. http://dx.doi.org/10.1115/icem2010-40284.

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This research addresses the potential of microbial implications in bentonite for use as a buffer and backfill material in final disposal site for low-level radioactive waste (LLRW) in Taiwan, where has a special island-type climate. Microbe activities naturally present in this site were analyzed, and buffer materials (BM) consisted of 100%, 70% or 50% bentonite were prepared for laboratory studies. A total of 39 microbial strains were isolated, and the predominant strains included four bacterial, one yeast and four fungal strains. Growth inhibition was not detected in any tested strain cultured in a radiation field with a dose rate of 0.2 Gy/h. Most of the isolated strains grew under a dose rate of 1.4 Gy/h. The D10 values of the tested strains ranged from 0.16 to 2.05 kGy. The mycelia of tested fungal strains could spread over 5 cm during six months of inoculation in BM. The spreading activity of the tested bacteria was less than that of the fungi. Moreover, biofilms were observed on the surfaces of the BM. Since a large and diverse population of microbes is present in Taiwan, microbes may contribute to the mobilization of radionuclides in the disposal site.
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Ferreira, A. A., R. C. S. Mendonça, H. M. Hungaro, M. M. Carvalho, and J. A. M. Pereira. "Bacteriophages actions on Salmonella Enteritidis biofilm." In Proceedings of the International Conference on Antimicrobial Research (ICAR2010). WORLD SCIENTIFIC, 2011. http://dx.doi.org/10.1142/9789814354868_0026.

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A. Rashid, Sirwan, Sawsan Muhammed Sorchee, Mustafa D. Yonus, and Omar F. Bahjat. "Identification Biofilm Producers s. Aureus Isolates and Detect their Biofilm Genes from Gingivitis Cases." In 4th International Conference on Biological & Health Sciences (CIC-BIOHS’2022). Cihan University, 2022. http://dx.doi.org/10.24086/biohs2022/paper.586.

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Gingivitis is one of the most common oral disorders and is caused by the accumulation of plaque in people with poor oral hygiene. The purpose of this study is to detect Staphylococcus aureus biofilm producers in cases of gingivitis and detect their biofilm genes. During the months of November 2018 and February 2019, a sample of fifty patients diagnosed clinically with gingivitis were collected from the laboratory of the Faculty of Dentistry at Hawler Medical University. Using the traditional culture method and vitek 2, eleven S. aureus bacteria isolates were identified. Using the Congo red agar method, biofilm detection was performed to test the ability to form biofilm. The S. aureus isolates were put through a series of tests to see if the genes responsible for the biofilm could be found (PCR). Biofilm formation method of detection using Congo red agar showed that 10 (90.1%) of S. aureus were biofilm positive and one 1 (9.1%) were biofilm negative. As well as the results of molecular analysis by using PCR showed that isolated S. aureus were carried biofilm genes icaC (100%), icaD (100%), cna (90.9%) and fnba (100%). Gingivitis is one of the most common oral diseases in our area, and this research found that the majority of S. aureus isolates carry the icaC, icaD, cna, and fnba Biofilm genes.
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Reports on the topic "Biofilms – Research"

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Wurl, Oliver. Biofilm-like habitat at the sea-surface: A mesocosm study, Cruise No. POS537, 14.09.2019 – 04.10.2019, Malaga (Spain) – Cartagena (Spain) - BIOFILM. University of Oldenburg, November 2020. http://dx.doi.org/10.3289/cr_pos537.

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OceanRep OceanRep Startseite Kontakt Schnellsuche Einfache Suche Erweiterte Suche Blättern Autor Forschungsbereich Publikationsart Jahr Studiengang Neuzugänge Artikel – begutachtet Alle Über uns GEOMAR Bibliothek Open Access Policies Grundsätze Hilfe FAQs Statistik Impressum Biofilm-like habitat at the sea-surface: A mesocosm study, Cruise No. POS537, 14.09.2019 – 04.10.2019, Malaga (Spain) – Cartagena (Spain) - BIOFILM . Logged in as Heidi Düpow Einträge verwaltenManage recordsManage shelvesProfilGespeicherte SuchenBegutachtungAdminLogout - Tools Wurl, Oliver, Mustaffa, Nur Ili Hamizah, Robinson, Tiera-Brandy, Hoppe, Jennifer, Jaeger, Leonie, Striebel, Maren, Heinrichs, Anna-Lena, Hennings, Laura Margarethe, Goncalves, Rodrigo, Ruiz Gazulla, Carlota und Ferrera, Isabel (2020) Biofilm-like habitat at the sea-surface: A mesocosm study, Cruise No. POS537, 14.09.2019 – 04.10.2019, Malaga (Spain) – Cartagena (Spain) - BIOFILM . Open Access . POSEIDON Berichte . University of Oldenburg, Oldenburg, 35 pp. [img] Text Cruise_Reports_POS537_final.pdf - publizierte Version Available under License Creative Commons: Attribution 4.0. Download (2417Kb) | Vorschau Abstract Biofilm-like properties can form on sea surfaces, but an understanding of the underlying processes leading to the development of these biofilms is not available. We used approaches to study the development of biofilm-like properties at the sea surface, i.e. the number, abundance and diversity of bacterial communities and phytoplankton, the accumulation of gel-like particles and dissolved tracers. During the expedition POS537 we used newly developed and free drifting mesocosms and performed incubation experiments. With these approaches we aim to investigate the role of light and UV radiation as well as the microbes themselves, which lead to the formation of biofilms. With unique microbial interactions and photochemical reactions, sea surface biofilms could be biochemical reactors with significant implications for ocean and climate research, e.g. with respect to the marine carbon cycle, diversity of organisms and oceanatmosphere interactions.
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Stanley-Wall, Nicola, and Joana Carneiro. Life of Bacteria over 200 degrees centigrade: Teachers' Guide. University of Dundee, 2022. http://dx.doi.org/10.20933/100001272.

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The “Life of bacteria over 200 degrees centigrade” video was created by the Public Engagement team at the University of Dundee’s School of Life Sciences, in collaboration with the Nicola-Stanley Wall Lab. This video follows a microbiologist performing an experiment in the laboratory and explains how scientists can study bacteria and biofilms. The video can be used by teachers to show their pupils how some microbial research is done in a professional laboratory environment. This guide helps teachers in this process.
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New, Mary. Annual progress Report on research related to our research project ?Stabilization of Plutonium in Subsurface Environments via Microbial Reduction and Biofilm Formation? funded by the Environmental Remediation Sciences Division (ERSD). Office of Scientific and Technical Information (OSTI), June 2006. http://dx.doi.org/10.2172/896425.

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Husson, Scott M., Viatcheslav Freger, and Moshe Herzberg. Antimicrobial and fouling-resistant membranes for treatment of agricultural and municipal wastewater. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7598151.bard.

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This research project introduced a novel membrane coating strategy to combat biofouling, which is a major problem for the membrane-based treatment of agricultural and municipal wastewaters. The novelty of the strategy is that the membrane coatings have the unique ability to switch reversibly between passive (antifouling) and active (antimicrobial) fouling control mechanisms. This dual-mode approach differs fundamentally from other coating strategies that rely solely on one mode of fouling control. The research project had two complementary objectives: (1) preparation, characterization, and testing of dual-mode polymer nanolayers on planar surfaces and (2) evaluation of these nanolayers as membrane modifiers. The first objective was designed to provide a fundamental understanding of how polymer nanolayer chemistry and structure affect bacterial deposition and to demonstrate the reversibility of chemical switching. The second objective, which focused on membrane development, characterization, and testing, was designed to demonstrate methods for the production of water treatment membranes that couple passive and active biofouling control mechanisms. Both objectives were attained through synergistic collaboration among the three research groups. Using planar silicon and glass surfaces, we demonstrated using infrared spectroscopy that this new polymer coating can switch reversibly between the anti-fouling, zwitterion mode and an anti-microbial, quaternary amine mode. We showed that switching could be done more than 50 times without loss of activity and that the kinetics for switching from a low fouling zwitterion surface to an antimicrobial quaternary amine surface is practical for use. While a low pH was required for switching in the original polymer, we illustrated that by slightly altering the chemistry, it is possible to adjust the pH at which the switching occurs. A method was developed for applying the new zwitterionic surface chemistry onto polyethersulfone (PES) ultrafiltration membranes. Bacteria deposition studies showed that the new chemistry performed better than other common anti-fouling chemistries. Biofilm studies showed that PESultrafiltration membranes coated with the new chemistry accumulated half the biomass volume as unmodified membranes. Biofilm studies also showed that PES membranes coated with the new chemistry in the anti-microbial mode attained higher biofilm mortality than PES membranes coated with a common, non-switchablezwitterionic polymer. Results from our research are expected to improve membrane performance for the purification of wastewaters prior to use in irrigation. Since reduction in flux due to biofouling is one of the largest costs associated with membrane processes in water treatment, using dual-mode nanolayer coatings that switch between passive and active control of biofouling and enable detachment of attached biofoulants would have significant economic and societal impacts. Specifically, this research program developed and tested advanced ultrafiltration membranes for the treatment of wastewaters. Such membranes could find use in membrane bioreactors treating municipal wastewater, a slightly upgraded version of what presently is used in Israel for irrigation. They also may find use for pretreatment of agricultural wastewaters, e.g., rendering facility wastewater, prior to reverse osmosis for desalination. The need to desalinate such impaired waters water for unlimited agricultural use is likely in the near future.
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Choudhary, Ruplal, Victor Rodov, Punit Kohli, Elena Poverenov, John Haddock, and Moshe Shemesh. Antimicrobial functionalized nanoparticles for enhancing food safety and quality. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7598156.bard.

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Original objectives The general goal of the project was to utilize the bactericidal potential of curcumin- functionalizednanostructures (CFN) for reinforcement of food safety by developing active antimicrobial food-contact surfaces. In order to reach the goal, the following secondary tasks were pursued: (a) further enhancement of the CFN activity based on understanding their mode of action; (b) preparing efficient antimicrobial surfaces, investigating and optimizing their performance; (c) testing the efficacy of the antimicrobial surfaces in real food trials. Background to the topic The project dealt with reducing microbial food spoilage and safety hazards. Cross-contamination through food-contact surfaces is one of the major safety concerns, aggravated by bacterial biofilm formation. The project implemented nanotech methods to develop novel antimicrobial food-contact materials based on natural compounds. Food-grade phenylpropanoidcurcumin was chosen as the most promising active principle for this research. Major conclusions, solutions, achievements In agreement with the original plan, the following research tasks were performed. Optimization of particles structure and composition. Three types of curcumin-functionalizednanostructures were developed and tested: liposome-type polydiacetylenenanovesicles, surface- stabilized nanoparticles and methyl-β-cyclodextrin inclusion complexes (MBCD). The three types had similar minimal inhibitory concentration but different mode of action. Nanovesicles and inclusion complexes were bactericidal while the nanoparticlesbacteriostatic. The difference might be due to different paths of curcumin penetration into bacterial cell. Enhancing the antimicrobial efficacy of CFN by photosensitization. Light exposure strengthened the bactericidal efficacy of curcumin-MBCD inclusion complexes approximately three-fold and enhanced the bacterial death on curcumin-coated plastic surfaces. Investigating the mode of action of CFN. Toxicoproteomic study revealed oxidative stress in curcumin-treated cells of E. coli. In the dark, this effect was alleviated by cellular adaptive responses. Under light, the enhanced ROS burst overrode the cellular adaptive mechanisms, disrupted the iron metabolism and synthesis of Fe-S clusters, eventually leading to cell death. Developing industrially-feasible methods of binding CFN to food-contact surfaces. CFN binding methods were developed for various substrates: covalent binding (binding nanovesicles to glass, plastic and metal), sonochemical impregnation (binding nanoparticles to plastics) and electrostatic layer-by-layer coating (binding inclusion complexes to glass and plastics). Investigating the performance of CFN-coated surfaces. Flexible and rigid plastic materials and glass coated with CFN demonstrated bactericidal activity towards Gram-negative (E. coli) and Gram-positive (Bac. cereus) bacteria. In addition, CFN-impregnated plastic material inhibited bacterial attachment and biofilm development. Testing the efficacy of CFN in food preservation trials. Efficient cold pasteurization of tender coconut water inoculated with E. coli and Listeriamonocytogeneswas performed by circulation through a column filled with CFN-coated glass beads. Combination of curcumin coating with blue light prevented bacterial cross contamination of fresh-cut melons through plastic surfaces contaminated with E. coli or Bac. licheniformis. Furthermore, coating of strawberries with CFN reduced fruit spoilage during simulated transportation extending the shelf life by 2-3 days. Implications, both scientific and agricultural BARD Report - Project4680 Page 2 of 17 Antimicrobial food-contact nanomaterials based on natural active principles will preserve food quality and ensure safety. Understanding mode of antimicrobial action of curcumin will allow enhancing its dark efficacy, e.g. by targeting the microbial cellular adaptation mechanisms.
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Brandl, Maria T., Shlomo Sela, Craig T. Parker, and Victor Rodov. Salmonella enterica Interactions with Fresh Produce. United States Department of Agriculture, September 2010. http://dx.doi.org/10.32747/2010.7592642.bard.

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The emergence of food-borne illness outbreaks linked to the contamination of fruits and vegetables is a great concern in industrialized countries. The current lack of control measures and effective sanitization methods prompt the need for new strategies to reduce contamination of produce. Our ability to assess the risk associated with produce contamination and to devise innovative control strategies depends on the identification of critical determinants that affect the growth and the persistence of human pathogens on plants. Salmonella enterica, a common causal agent of illness linked to produce, has the ability to colonize and persist on plants. Thus, our main objective was to identify plant-inducible genes that have a role in the growth and/or persistence of S. enterica on postharvest lettuce. Our findings suggest that in-vitro biofilm formation tests may provide a suitable model to predict the initial attachment of Salmonella to cut-romaine lettuce leaves and confirm that Salmonella could persist on lettuce during shelf-life storage. Importantly, we found that Salmonella association with lettuce increases its acid-tolerance, a trait which might be correlated with an enhanced ability of the pathogen to pass through the acidic barrier of the stomach. We have demonstrated that Salmonella can internalize leaves of iceberg lettuce through open stomata. We found for the first time that internalization is an active bacterial process mediated by chemotaxis and motility toward nutrient produced in the leaf by photosynthesis. These findings may provide a partial explanation for the failure of sanitizers to efficiently eradicate foodborne pathogens in leafy greens and may point to a novel mechanism utilized by foodborne and perhaps plant pathogens to colonize leaves. Using resolvase in vivo expression technology (RIVET) we have managed to identify multiple Salmonella genes, some of which with no assigned function, which are involved in attachment to and persistence of Salmonella on lettuce leaves. The precise function of these genes in Salmonella-leaf interactions is yet to be elucidated. Taken together, our findings have advanced the understanding of how Salmonella persist in the plant environment, as well as the potential consequences upon ingestion by human. The emerging knowledge opens new research directions which should ultimately be useful in developing new strategies and approaches to reduce leaf contamination and enhance the safety of fresh produce.
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Manulis-Sasson, Shulamit, Christine D. Smart, Isaac Barash, Laura Chalupowicz, Guido Sessa, and Thomas J. Burr. Clavibacter michiganensis subsp. michiganensis-tomato interactions: expression and function of virulence factors, plant defense responses and pathogen movement. United States Department of Agriculture, February 2015. http://dx.doi.org/10.32747/2015.7594405.bard.

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Clavibactermichiganensissubsp. michiganensis(Cmm), the causal agent of bacterial wilt and canker of tomato, is the most destructive bacterial disease of tomato causing substantial economic losses in Israel, the U.S.A. and worldwide. The goal of the project was to unravel the molecular strategies that allow Cmm, a Gram-positive bacterium, to develop a successful infection in tomato. The genome of Cmm contains numerous genes encoding for extracellular serine proteases and cell wall degrading enzymes. The first objective was to elucidate the role of secreted serine proteases in Cmm virulence. Mutants of nine genes encoding serine proteases of 3 different families were tested for their ability to induce wilting, when tomato stems were puncture-inoculated, as compared to blisters formation on leaves, when plants were spray-inoculated. All the mutants showed reduction in wilting and blister formation as compared to the wild type. The chpCmutant displayed the highest reduction, implicating its major role in symptom development. Five mutants of cell wall degrading enzymes and additional genes (i.e. perforin and sortase) caused wilting but were impaired in their ability to form blisters on leaves. These results suggest that Cmm differentially expressed virulence genes according to the site of penetration. Furthermore, we isolated and characterized two Cmmtranscriptional activators, Vatr1 and Vatr2 that regulate the expression of virulence factors, membrane and secreted proteins. The second objective was to determine the effect of bacterial virulence genes on movement of Cmm in tomato plants and identify the routes by which the pathogen contaminates seeds. Using a GFP-labeledCmm we could demonstrate that Cmm extensively colonizes the lumen of xylem vessels and preferentially attaches to spiral secondary wall thickening of the protoxylem and formed biofilm-like structures composed of large bacterial aggregates. Our findings suggest that virulence factors located on the chp/tomAPAI or the plasmids are required for effective movement of the pathogen in tomato and for the formation of cellular aggregates. We constructed a transposon plasmid that can be stably integrated into Cmm chromosome and express GFP, in order to follow movement to the seeds. Field strains from New York that were stably transformed with this construct, could not only access seeds systemically through the xylem, but also externally through tomato fruit lesions, which harbored high intra-and intercellular populations. Active movement and expansion of bacteria into the fruit mesocarp and nearby xylem vessels followed, once the fruit began to ripen. These results highlight the ability of Cmm to invade tomato fruit and seed through multiple entry routes. The third objective was to assess correlation between disease severity and expression levels of Cmm virulence genes and tomato defense genes. The effect of plant age on expression of tomato defense related proteins during Cmm infection was analyzed by qRT-PCR. Five genes out of eleven showed high induction at early stages of infection of plants with 19/20 leaves compared to young plants bearing 7/8 leaves. Previous results showed that Cmm virulence genes were expressed at early stages of infection in young plants compared to older plants. Results of this study suggest that Cmm virulence genes may suppress expression of tomato defense-related genes in young plants allowing effective disease development. The possibility that chpCis involved in suppression of tomato defense genes is currently under investigation by measuring the transcript level of several PR proteins, detected previously in our proteomics study. The fourth objective was to define genome location and stability of virulence genes in Cmm strains. New York isolates were compared to Israeli, Serbian, and NCPPB382 strains. The plasmid profiles of New York isolates were diverse and differed from both Israeli and Serbian strains. PCR analysis indicated that the presence of putative pathogenicity genes varied between isolates and highlighted the ephemeral nature of pathogenicity genes in field populations of Cmm. Results of this project significantly contributed to the understanding of Cmm virulence, its movement within tomato xylem or externally into the seeds, the role of serine proteases in disease development and initiated research on global regulation of Cmm virulence. These results form a basis for developing new strategies to combat wilt and canker disease of tomato.
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