Academic literature on the topic 'Biofilms marins'
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Journal articles on the topic "Biofilms marins"
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Sousa-Cardoso, Francisca, Rita Teixeira-Santos, Ana Francisca Campos, Marta Lima, Luciana C. Gomes, Olívia S. G. P. Soares, and Filipe J. Mergulhão. "Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments." Nanomaterials 13, no. 3 (January 18, 2023): 381. http://dx.doi.org/10.3390/nano13030381.
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Due to its several economic and ecological consequences, biofouling is a widely recognized concern in the marine sector. The search for non-biocide-release antifouling coatings has been on the rise, with carbon-nanocoated surfaces showing promising activity. This work aimed to study the impact of pristine graphene nanoplatelets (GNP) on biofilm development through the representative marine bacteria Cobetia marina and to investigate the antibacterial mechanisms of action of this material. For this purpose, a flow cytometric analysis was performed and a GNP/polydimethylsiloxane (PDMS) surface containing 5 wt% GNP (G5/PDMS) was produced, characterized, and assessed regarding its biofilm mitigation potential over 42 days in controlled hydrodynamic conditions that mimic marine environments. Flow cytometry revealed membrane damage, greater metabolic activity, and endogenous reactive oxygen species (ROS) production by C. marina when exposed to GNP 5% (w/v) for 24 h. In addition, C. marina biofilms formed on G5/PDMS showed consistently lower cell count and thickness (up to 43% reductions) than PDMS. Biofilm architecture analysis indicated that mature biofilms developed on the graphene-based surface had fewer empty spaces (34% reduction) and reduced biovolume (25% reduction) compared to PDMS. Overall, the GNP-based surface inhibited C. marina biofilm development, showing promising potential as a marine antifouling coating.
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Liang, Xiao, Xiu-Kun Zhang, Li-Hua Peng, You-Ting Zhu, Asami Yoshida, Kiyoshi Osatomi, and Jin-Long Yang. "The Flagellar Gene Regulates Biofilm Formation and Mussel Larval Settlement and Metamorphosis." International Journal of Molecular Sciences 21, no. 3 (January 21, 2020): 710. http://dx.doi.org/10.3390/ijms21030710.
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Biofilms are critical components of most marine systems and provide biochemical cues that can significantly impact overall community composition. Although progress has been made in the bacteria–animal interaction, the molecular basis of modulation of settlement and metamorphosis in most marine animals by bacteria is poorly understood. Here, Pseudoalteromonas marina showing inducing activity on mussel settlement and metamorphosis was chosen as a model to clarify the mechanism that regulates the bacteria–mussel interaction. We constructed a flagellin synthetic protein gene fliP deletion mutant of P. marina and checked whether deficiency of fliP gene will impact inducing activity, motility, and extracellular polymeric substances of biofilms. Furthermore, we examined the effect of flagellar proteins extracted from bacteria on larval settlement and metamorphosis. The deletion of the fliP gene caused the loss of the flagella structure and motility of the ΔfliP strain. Deficiency of the fliP gene promoted the biofilm formation and changed biofilm matrix by reducing β-polysaccharides and increasing extracellular proteins and finally reduced biofilm-inducing activities. Flagellar protein extract promoted mussel metamorphosis, and ΔfliP biofilms combined with additional flagellar proteins induced similar settlement and metamorphosis rate compared to that of the wild-type strain. These findings provide novel insight on the molecular interactions between bacteria and mussels.
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Long, Lexin, Ruojun Wang, Ho Yin Chiang, Wei Ding, Yong-Xin Li, Feng Chen, and Pei-Yuan Qian. "Discovery of Antibiofilm Activity of Elasnin against Marine Biofilms and Its Application in the Marine Antifouling Coatings." Marine Drugs 19, no. 1 (January 5, 2021): 19. http://dx.doi.org/10.3390/md19010019.
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Biofilms are surface-attached multicellular communities that play critical roles in inducing biofouling and biocorrosion in the marine environment. Given the serious economic losses and problems caused by biofouling and biocorrosion, effective biofilm control strategies are highly sought after. In a screening program of antibiofilm compounds against marine biofilms, we discovered the potent biofilm inhibitory activity of elasnin. Elasnin effectively inhibited the biofilm formation of seven strains of bacteria isolated from marine biofilms. With high productivity, elasnin-based coatings were prepared in an easy and cost-effective way, which exhibited great performance in inhibiting the formation of multi-species biofilms and the attachment of large biofouling organisms in the marine environment. The 16S amplicon analysis and anti-larvae assay revealed that elasnin could prevent biofouling by the indirect impact of changed microbial composition of biofilms and direct inhibitory effect on larval settlement with low toxic effects. These findings indicated the potential application of elasnin in biofilm and biofouling control in the marine environment.
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Tuck, Benjamin, Silvia J. Salgar-Chaparro, Elizabeth Watkin, Anthony Somers, Maria Forsyth, and Laura L. Machuca. "Extracellular DNA: A Critical Aspect of Marine Biofilms." Microorganisms 10, no. 7 (June 24, 2022): 1285. http://dx.doi.org/10.3390/microorganisms10071285.
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Multispecies biofilms represent a pervasive threat to marine-based industry, resulting in USD billions in annual losses through biofouling and microbiologically influenced corrosion (MIC). Biocides, the primary line of defence against marine biofilms, now face efficacy and toxicity challenges as chemical tolerance by microorganisms increases. A lack of fundamental understanding of species and EPS composition in marine biofilms remains a bottleneck for the development of effective, target-specific biocides with lower environmental impact. In the present study, marine biofilms are developed on steel with three bacterial isolates to evaluate the composition of the EPSs (extracellular polymeric substances) and population dynamics. Confocal laser scanning microscopy, scanning electron microscopy, and fluorimetry revealed that extracellular DNA (eDNA) was a critical structural component of the biofilms. Parallel population analysis indicated that all three strains were active members of the biofilm community. However, eDNA composition did not correlate with strain abundance or activity. The results of the EPS composition analysis and population analysis reveal that biofilms in marine conditions can be stable, well-defined communities, with enabling populations that shape the EPSs. Under marine conditions, eDNA is a critical EPS component of the biofilm and represents a promising target for the enhancement of biocide specificity against these populations.
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Faria, Sara I., Rita Teixeira-Santos, Maria J. Romeu, João Morais, Vitor Vasconcelos, and Filipe J. Mergulhão. "The Relative Importance of Shear Forces and Surface Hydrophobicity on Biofilm Formation by Coccoid Cyanobacteria." Polymers 12, no. 3 (March 12, 2020): 653. http://dx.doi.org/10.3390/polym12030653.
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Understanding the conditions affecting cyanobacterial biofilm development is crucial to develop new antibiofouling strategies and decrease the economic and environmental impact of biofilms in marine settings. In this study, we investigated the relative importance of shear forces and surface hydrophobicity on biofilm development by two coccoid cyanobacteria with different biofilm formation capacities. The strong biofilm-forming Synechocystis salina was used along with the weaker biofilm-forming Cyanobium sp. Biofilms were developed in defined hydrodynamic conditions using glass (a model hydrophilic surface) and a polymeric epoxy coating (a hydrophobic surface) as substrates. Biofilms developed in both surfaces at lower shear conditions contained a higher number of cells and presented higher values for wet weight, thickness, and chlorophyll a content. The impact of hydrodynamics on biofilm development was generally stronger than the impact of surface hydrophobicity, but a combined effect of these two parameters strongly affected biofilm formation for the weaker biofilm-producing organism. The antibiofilm performance of the polymeric coating was confirmed at the hydrodynamic conditions prevailing in ports. Shear forces were shown to have a profound impact on biofilm development in marine settings regardless of the fouling capacity of the existing flora and the hydrophobicity of the surface.
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Dheilly, Alexandra, Emmanuelle Soum-Sout�ra, G�raldine L. Klein, Alexis Bazire, Chantal Comp�re, Dominique Haras, and Alain Dufour. "Antibiofilm Activity of the Marine Bacterium Pseudoalteromonas sp. Strain 3J6." Applied and Environmental Microbiology 76, no. 11 (April 2, 2010): 3452–61. http://dx.doi.org/10.1128/aem.02632-09.
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ABSTRACT Biofilm formation results in medical threats or economic losses and is therefore a major concern in a variety of domains. In two-species biofilms of marine bacteria grown under dynamic conditions, Pseudoalteromonas sp. strain 3J6 formed mixed biofilms with Bacillus sp. strain 4J6 but was largely predominant over Paracoccus sp. strain 4M6 and Vibrio sp. strain D01. The supernatant of Pseudoalteromonas sp. 3J6 liquid culture (SN3J6) was devoid of antibacterial activity against free-living Paracoccus sp. 4M6 and Vibrio sp. D01 cells, but it impaired their ability to grow as single-species biofilms and led to higher percentages of nonviable cells in 48-h biofilms. Antibiofilm molecules of SN3J6 were able to coat the glass surfaces used to grow biofilms and reduced bacterial attachment about 2-fold, which might partly explain the biofilm formation defect but not the loss of cell viability. SN3J6 had a wide spectrum of activity since it affected all Gram-negative marine strains tested except other Pseudoalteromonas strains. Biofilm biovolumes of the sensitive strains were reduced 3- to 530-fold, and the percentages of nonviable cells were increased 3- to 225-fold. Interestingly, SN3J6 also impaired biofilm formation by three strains belonging to the human-pathogenic species Pseudomonas aeruginosa, Salmonella enterica, and Escherichia coli. Such an antibiofilm activity is original and opens up a variety of applications for Pseudoalteromonas sp. 3J6 and/or its active exoproducts in biofilm prevention strategies.
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Mai-Prochnow, Anne, Flavia Evans, Doralyn Dalisay-Saludes, Sacha Stelzer, Suhelen Egan, Sally James, Jeremy S. Webb, and Staffan Kjelleberg. "Biofilm Development and Cell Death in the Marine Bacterium Pseudoalteromonas tunicata." Applied and Environmental Microbiology 70, no. 6 (June 2004): 3232–38. http://dx.doi.org/10.1128/aem.70.6.3232-3238.2004.
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ABSTRACT The newly described green-pigmented bacterium Pseudoalteromonas tunicata (D2) produces target-specific inhibitory compounds against bacteria, algae, fungi, and invertebrate larvae and is frequently found in association with living surfaces in the marine environment. As part of our studies on the ecology of P. tunicata and its interaction with marine surfaces, we examined the ability of P. tunicata to form biofilms under continuous culture conditions within the laboratory. P. tunicata biofilms exhibited a characteristic architecture consisting of differentiated microcolonies surrounded by water channels. Remarkably, we observed a repeatable pattern of cell death during biofilm development of P. tunicata, similar to that recently reported for biofilms of Pseudomonas aeruginosa (J. S. Webb et al., J. Bacteriol. 185:4585-4595, 2003). Killing and lysis occurred inside microcolonies, apparently resulting in the formation of voids within these structures. A subpopulation of viable cells was always observed within the regions of killing in the biofilm. Moreover, extensive killing in mature biofilms appeared to result in detachment of the biofilm from the substratum. A novel 190-kDa autotoxic protein produced by P. tunicata, designated AlpP, was found to be involved in this biofilm killing and detachment. A ΔalpP mutant derivative of P. tunicata was generated, and this mutant did not show cell death during biofilm development. We propose that AlpP-mediated cell death plays an important role in the multicellular biofilm development of P. tunicata and subsequent dispersal of surviving cells within the marine environment.
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Gill, Stephanie P., Louise Kregting, Ibrahim M. Banat, Joerg Arnscheidt, and William R. Hunter. "Rhamnolipids Mediate the Effects of a Gastropod Grazer in Regards to Carbon–Nitrogen Stoichiometry of Intertidal Microbial Biofilms." Applied Sciences 12, no. 24 (December 12, 2022): 12729. http://dx.doi.org/10.3390/app122412729.
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Microbial biofilms have co-evolved with grazing animals, such as gastropods, to develop mutually beneficial relationships. Although microbial biofilms demonstrate resilience and resistance to chemical exposure, pre-existing relationships can be negatively affected by chemical input. In this study, we determined how the grazer, Littorina littorea (common periwinkle sea snail), and a biological surfactant (rhamnolipid) interact on a phototrophic marine biofilm. Biofilms were cultured in 32 twenty-liter buckets at the Queen’s University Marine Laboratory in Portaferry, Northern Ireland on clay tiles that were either exposed to 150 ppm of a rhamnolipid solution or that had no chemical exposure. L. littorea were added into half of the buckets, and biofilms were developed over 14 days. Biofilms exposed to grazing alone demonstrated high tolerance to the disturbance, while those growing on rhamnolipid-exposed substrate demonstrated resistance but experienced slight declines in carbon and stoichiometric ratios. However, when exposed to both, biofilms had significant decreases in stoichiometry and declined in productivity and respiration. This is problematic, as continuing marine pollution increases the likelihood that biofilms will be exposed to combinations of stressors and disturbances. Loss of biofilm productivity within these areas could lead to the loss of an important food source and nutrient cycler within the marine ecosystem.
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Faria, Sara I., Rita Teixeira-Santos, Maria J. Romeu, João Morais, Ed de Jong, Jelmer Sjollema, Vítor Vasconcelos, and Filipe J. Mergulhão. "Unveiling the Antifouling Performance of Different Marine Surfaces and Their Effect on the Development and Structure of Cyanobacterial Biofilms." Microorganisms 9, no. 5 (May 20, 2021): 1102. http://dx.doi.org/10.3390/microorganisms9051102.
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Since biofilm formation by microfoulers significantly contributes to the fouling process, it is important to evaluate the performance of marine surfaces to prevent biofilm formation, as well as understand their interactions with microfoulers and how these affect biofilm development and structure. In this study, the long-term performance of five surface materials—glass, perspex, polystyrene, epoxy-coated glass, and a silicone hydrogel coating—in inhibiting biofilm formation by cyanobacteria was evaluated. For this purpose, cyanobacterial biofilms were developed under controlled hydrodynamic conditions typically found in marine environments, and the biofilm cell number, wet weight, chlorophyll a content, and biofilm thickness and structure were assessed after 49 days. In order to obtain more insight into the effect of surface properties on biofilm formation, they were characterized concerning their hydrophobicity and roughness. Results demonstrated that silicone hydrogel surfaces were effective in inhibiting cyanobacterial biofilm formation. In fact, biofilms formed on these surfaces showed a lower number of biofilm cells, chlorophyll a content, biofilm thickness, and percentage and size of biofilm empty spaces compared to remaining surfaces. Additionally, our results demonstrated that the surface properties, together with the features of the fouling microorganisms, have a considerable impact on marine biofouling potential.
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Dusane, D. H., Y. V. Nancharaiah, V. P. Venugopalan, A. R. Kumar, and S. S. Zinjarde. "Biofilm formation by a biotechnologically important tropical marine yeast isolate, Yarrowia lipolytica NCIM 3589." Water Science and Technology 58, no. 6 (October 1, 2008): 1221–29. http://dx.doi.org/10.2166/wst.2008.479.
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Biofilm formation by Yarrowia lipolytica, a biotechnologically important fungus in microtitre plates, on glass slide surfaces and in flow cell was investigated. In microtitre plates, there was a short lag phase of adhesion followed by a period of rapid biofilm growth. The fungus formed extensive biofilms on glass slides, whereas in flow-cells a multicellular, three-dimensional microcolony structure was observed. The isolate formed biofilms in seawater and in fresh water media at neutral pH when grown in microtitre plates. The carbon sources differentially affected formation of biofilms in microtitre plates. Lactic acid, erythritol, glycerol, glucose and edible oils supported the formation of biofilms, while alkanes resulted in sub-optimal biofilm development. A variation in the morphology of the fungus was observed with different carbon sources. The results point to the possible existence of highly structured biofilms in varied ecological niches from where the yeast is isolated.
Dissertations / Theses on the topic "Biofilms marins"
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Passarelli, Claire. "Composition, rôles et devenir des exopolymères dans les biofilms des sédiments marins côtiers." Paris, Muséum national d'histoire naturelle, 2013. http://www.theses.fr/2013MNHN0012.
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Carriot, Nathan. "Caractérisation de la production métabolique de biofilms marins. : Vers une application à l'étude de biofilms complexes in situ." Electronic Thesis or Diss., Toulon, 2022. http://www.theses.fr/2022TOUL0001.
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Le phénomène de biofouling est un processus naturel qui impacte toutes les surfaces immergées en milieu marin engendrant des problèmes économiques et écologiques majeurs à l’échelle planétaire. Il est notamment induit par la formation de biofilms marins correspondant à la colonisation des surfaces immergées par des bactéries s’organisant en communautés en s’entourant d’une matrice de substances polymériques extracellulaires (EPS). L’objectif de ce travail est l’utilisation et le développement de méthodologies permettant l’étude et la compréhension de l’étape précurseur de ce phénomène. La corrélation des données récoltées à partir des méthodes appliquées (métabolomique et réseau moléculaire, protéomique, dosages colorimétriques, microscopies, spectroscopies) permet une approche multi-échelles pour la caractérisation des biofilms. Ces développements visent, en premier lieu, à caractériser la production biochimique globale de biofilms in vitro pour ensuite analyser des biofilms naturels formés in situ. L’utilisation de ce large panel de techniques a permis de répondre à certaines questions scientifiques comme l’impact des nutriments (phosphates), d’une enzyme (quorum sensing) ou de l’hydrodynamisme sur la nature de biofilms formésThe phenomenon of biofouling is a natural process that impacts all the surfaces submerged in the marine environment, generating major economic and ecological problems on a global scale. It is induced by the formation of marine biofilms corresponding to the colonization of submerged surfaces by bacteria organizing in communities by surrounding themselves with a matrix of extracellular polymeric substances (EPS). The objective of this work is the use and development of methodologies to study and understand the precursor stage of this phenomenon. The correlation of the data collected from the applied methods (metabolomics and molecular network, proteomics, colorimetric assays, microscopies, spectroscopy) allows a multi-scale approach for the characterization of biofilms. These developments aim, first of all, to characterize the overall biochemical production of in vitro biofilms and then analyse natural biofilms formed in situ. The use of this wide range of techniques has made it possible to answer certain scientific questions such as the impact of nutrients (phosphates), an enzyme (quorum sensing) or hydrodynamics on the nature of formed biofilms
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Dheilly, Alexandra. "Biofilms bactériens marins multi-espèces : mise en évidence d'un effet antagoniste." Lorient, 2007. http://www.theses.fr/2007LORIS091.
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Les salissures marines ou biofouling sont à l’origine de problèmes écologiques et de pertes économiques. L’une des premières étapes intervenant dans le biofouling est la formation de biofilms bactériens, c'est-à-dire de communautés de bactéries adhérées irréversiblement à un support et enveloppées dans une matrice de polymères extracellulaires. De plus en plus de travaux ont pour but d’étudier ces communautés bactériennes dans la perspective de contrôler, d’éviter ou de limiter leur formation. Ce travail a été consacré à l’étude in vitro du développement de biofilms marins pluri-espèces dans un système dynamique, et à la relation existant entre la formation du biofilm et les propriétés phénotypiques des différentes souches bactériennes utilisées. Deux étapes clés de la formation d’un biofilm bactérien ont été investiguées : l’adhésion des bactéries et la croissance du biofilm. L’étude des biofilms mono-espèces suggère que la mobilité et la production d’exoproduits par les bactéries induisent l’établissement de biofilms plus épais formant des agrégats bactériens. En revanche, nos résultats n’ont pas permis d’établir un lien entre la structure des biofilms et la production ou non de molécules de communication de type N-acyl-L-homoserine lactone ou furanosyl borate diester. Des biofilms pluri-espèces ont été réalisés à partir de trois couples bactériens, tous contenant Pseudoalteromonas 3J6. Les résultats montrent que le couple Pseudoalteromonas 3J6-Bacillus 4J6 produit un biofilm mixte (Bacillus 4J6 : 38 %, Pseudoalteromonas 3J6 : 62 %), dont les cellules de Bacillus 4J6 sont localisées tout autour des microcolonies de Pseudoalteromonas 3J6. En revanche, la bactérie Pseudoalteromonas 3J6 est très largement majoritaire (90-95 %) dans les biofilms résultant de son inoculation avec Vibrio D01 ou Paracoccus 4M6. Ceci met en évidence un effet antagoniste de Pseudoalteromonas 3J6 vis-à-vis de ces deux souches bactériennes. Le mécanisme d’inhibition de Pseudoalteromonas 3J6 a ensuite été analysé sur les cellules bactériennes planctoniques et sessiles. Les résultats suggèrent un effet spécifiquement anti-biofilm dû aux composés extracellulaires produits par cette souche. Ces composés, probablement de nature protéique, agissent dès la première étape du biofilm en diminuant l’adhésion des cellules au support. Enfin, les effets de ces composés extracellulaires montrent des similarités à ceux obtenus avec deux biocides commerciaux, molécules utilisées dans les peintures antifouling. En effet, l’ajout de biocides diminue le pouvoir d’adhésion des bactéries et inhibe la croissance du biofilm. En outre, les biocides augmentent la mortalité des cellules au sein du biofilmMarine fouling or biofouling leads to ecologic damages and economic losses. One of the earliest stages of biofouling is the formation of a bacterial biofilm, consisting of an assemblage of irreversibly adhered microbial cells on a surface, and enclosed in a hydrated matrix of extra-cellular polymeric substances. Many works currently aim at studying biofilm development in order to control, prevent or limit their formation. In this work, we investigated the in vitro development of multi-species biofilms of marine bacteria in a dynamic system, and the relationship between biofilm formation and phenotypic properties of each bacterial strain used. Biofilm formation was analysed at two levels: cell adhesion and biofilm growth. Study of mono-species biofilms suggested that motility and exopolymer production by bacteria induce thick biofilms contains microcolonies. However, our results did not allow to establish a link between biofilm structure and ability of the bacteria to produce or not communication molecules such as N-acyl-L-homoserine lactone or furanosyl borate diester. Mixed biofilms were produced from three strain couples, all of them including Pseudoalteromonas 3J6. The results show that in Pseudoalteromonas-Bacillus biofilms, Bacillus 4J6 cells were located in between Pseudoalteromonas 3J6 microcolonies (Bacillus 4J6: 38 %, Pseudoalteromonas 3J6: 62 %). By contrast, development of Vibrio D01 and Paracoccus 4M6 strains was strongly impaired in Pseudoalteromonas-containing biofilms (Pseudoalteromonas 3J6: 90/95 %). Theses results revealed an inhibitory effect of Pseudoalteromonas 3J6 towards Vibrio D01 and Paracoccus 4M6 strains. The inhibition mechanism of Pseudoalteromonas 3J6 was then studied on planktonic and sessile cells. The results suggest a biofilm-specific effect relying on extracellular compounds produced by this bacterium. Also, we observed that theses compounds are probably of proteic nature and that they affect the first step of biofilm formation by impairing the cell adhesion onto the surface. Finally, there was a similarity between effects of Pseudoalteromonas 3J6 exoproducts and commercials biocides, which are used in antifouling paints. Indeed, addition of biocides led to diminution of cell adhesion power and inhibition of biofilm growth. Besides, biocides increased mortality inside biofilms
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Favre, Laurie. "Caractérisation par analyse métabolomique de biomarqueurs bactériens au sein de biofilms marins." Thesis, Toulon, 2017. http://www.theses.fr/2017TOUL0005/document.
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En milieu marin, toute surface immergée est soumise à une colonisation par de nombreux organismes (biofouling). Le développement de biofilms est une étape clé du phénomène. Les systèmes de communication y sont contrôlés par le biais de signaux chimiques. Dans ce travail, l’étude de la signature métabolique de biofilms naturels formé in situ a été réalisée selon un gradient de pollution en contaminants métalliques dans la rade de Toulon et selon la nature du revêtement de la surface immergée. De nettes variations chimiques des biofilms prélevés sont observées et sont corrélées avec des variations en termes de communauté microbiennes. L’étude in vitro de 4 souches bactériennes issues de biofilms naturels a permis, après optimisation des méthodologies d’analyse, une discrimination selon leur profil métabolique. Des biomarqueurs ont été mis en évidence, avec notamment la production de lipides ornithine par la souche Pseudoalteromonas lipolytica. La réponse biologique de cette souche en fonction de son phénotype et face à un stress cuprique a été étudiée par métabolomique et protéomique révélant d’importantes modulations de certaines voies biosynthétiquesIn the marine environment, any immersed surface is subjected to colonization by many organisms (biofouling). The biofilms development is a key stage of this phenomenon. Communication systems are controlled in these structures by chemical signals. In this work, the study of the chemical signature of natural biofilms formed in situ was carried out among a gradient of contamination of metal contaminants in the bay of Toulon and according to the nature of the coating on the immersed surface. Clear chemical variations of the biofilms collected were observed and were correlated with variations in microbial community. The in vitro study of 4 bacterial strains harvested from natural biofilms allowed, after optimization of the analysis methodologies, their discrimination according to their metabolic profile. Biomarkers were highlited, particularly ornithine lipids production by the Pseudoalteromonas lipolytica strain. The biological response of this strain depending on its phenotype and face to copper stres was studied by metabolomics and proteomics revealing important modulations of certain biosynthetic patways
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Favre, Laurie. "Caractérisation par analyse métabolomique de biomarqueurs bactériens au sein de biofilms marins." Electronic Thesis or Diss., Toulon, 2017. http://www.theses.fr/2017TOUL0005.
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En milieu marin, toute surface immergée est soumise à une colonisation par de nombreux organismes (biofouling). Le développement de biofilms est une étape clé du phénomène. Les systèmes de communication y sont contrôlés par le biais de signaux chimiques. Dans ce travail, l’étude de la signature métabolique de biofilms naturels formé in situ a été réalisée selon un gradient de pollution en contaminants métalliques dans la rade de Toulon et selon la nature du revêtement de la surface immergée. De nettes variations chimiques des biofilms prélevés sont observées et sont corrélées avec des variations en termes de communauté microbiennes. L’étude in vitro de 4 souches bactériennes issues de biofilms naturels a permis, après optimisation des méthodologies d’analyse, une discrimination selon leur profil métabolique. Des biomarqueurs ont été mis en évidence, avec notamment la production de lipides ornithine par la souche Pseudoalteromonas lipolytica. La réponse biologique de cette souche en fonction de son phénotype et face à un stress cuprique a été étudiée par métabolomique et protéomique révélant d’importantes modulations de certaines voies biosynthétiquesIn the marine environment, any immersed surface is subjected to colonization by many organisms (biofouling). The biofilms development is a key stage of this phenomenon. Communication systems are controlled in these structures by chemical signals. In this work, the study of the chemical signature of natural biofilms formed in situ was carried out among a gradient of contamination of metal contaminants in the bay of Toulon and according to the nature of the coating on the immersed surface. Clear chemical variations of the biofilms collected were observed and were correlated with variations in microbial community. The in vitro study of 4 bacterial strains harvested from natural biofilms allowed, after optimization of the analysis methodologies, their discrimination according to their metabolic profile. Biomarkers were highlited, particularly ornithine lipids production by the Pseudoalteromonas lipolytica strain. The biological response of this strain depending on its phenotype and face to copper stres was studied by metabolomics and proteomics revealing important modulations of certain biosynthetic patways
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Doiron, Kim. "Approches dynamiques et fondamentales de la formation des biofilms marins mono et multi espèces." Lorient, 2011. http://www.theses.fr/2011LORIS234.
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Les biofilms se retrouvent dans de nombreux environnements où ils sont représentés par une ou plusieurs espèces. Pour mieux comprendre la formation des biofilms marins, quatre modèles d’études ont été sélectionnés: la bactérie Pseudoalteromonas 3J6, les Diatomées Amphora coffeaeformis et Cylindrotheca closterium et le dinoflagellé Prorocentrum lima. Les biofilms ont été cultivés dans un bioréacteur contenant 2 litres d’eau de mer artificielle durant 22 jours. Des analyses en CMF, CPG, FTIR, MALDI-TOF, MCBL, MEB et CL-SM/SM ont été effectuées pour interpréter les biofilms. Les résultats démontrent une libération de substances polymériques extracellulaires différente selon la nature des micro-organismes et le nombre d’espèces présent dans les biofilms. Les images en MCBL montrent un arrangement cellulaire distinct entre la niche écologique occupée par les bactéries et celles par les microalgues. Les espèces P. 3J6 et A. Coffeaeformis occuperaient le même espace conduisant à une compétition pour la ressource nutritive. À l’inverse, une coopération est observée pour les espèces de P. 3J6 et C. Closterium. Aussi, la production d’acide okadaïque par P. Lima ne semble pas être affectée par la présence de P. 3J6 et une diminution de l’adhésion de P. Lima au contact de P. 3J6 semble favorisée. Ainsi, les biofilms ne se forment pas de façon identique selon les espèces et leur contribution relative varie suivant leur degré d’occupation sur les supports. Ces nouvelles approches expérimentales pourront trouver une application dans les grands domaines comme l’aquaculture, la santé humaine, l’agroalimentaire, mais également dans la recherche et dans la structuration des biofilmsBiofilms are found in many environments where they are represented by one or more species. To better understand the formation of marine biofilms four study models were selected: the bacterium Pseudoalteromonas 3J6, diatoms Amphora coffeaeformis and Cylindrotheca closterium, the dinoflagellate Prorocentrum lima. Biofilms were grown in a bioreactor containing 2 L of artificial sea water for 22 days. Analysis by MFC, GC, FTIR, MALDI-TOF, CLSM, SEM AND LC-SM/SM were performed to interpret the biofilms. The results demonstrate a release of extracellular polymeric substances differently depending on the nature of microorganisms and the number of species present in biofilm. CLSM imqges show a distinct cellular arrangement between the ecological niche occupied by bacteria those from microalgae. The species P. 3J6 and A. Coffeaeformis occupy the same space, leading to competition for nutrient resources. Conversely, cooperation is observed for the species P. 3J6 and C. Closterium. Thus, okadaic acid production by P. Lima does not seem to be affected by the presence of P. 3J6 and decrease adherence of P. Lima in contact P. 3J6 seems favored. Thus, biofilms are not formed in identical among species and their relative contribution varies according to their degree of occupancy on the supports. These new experimental approaches may find application in large areas such as aquaculture, human health, food, but also in research and in the structuring of biofilms
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Abi, Nassif Léa. "Elaboration et caractérisation de biomatériaux antimicrobiens à base d'alginate pour des applications dans les domaines médicaux et marins." Thesis, Brest, 2019. http://www.theses.fr/2019BRES0007/document.
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Le phénomène de biofouling affecte toute surface immergée dans un milieu aqueux, le recouvrant par un biofilm que ce soit dans le milieu médical ou marin. Afin de lutter contre ce phénomène, lors de ce travail, des matériaux biosourcés à base d’alginate ont été élaborés. Pour des applications dans le milieu médical, des films d’hydrogel de surface lisse ou poreuse et des films déposés sur acier par électrophorèse ont été élaborés. La présence de cuivre et zinc sous leur forme ionique Cu2+ et Zn2+ a été démontrée. Tous les films à base de cuivre et de zinc ont montré des propriétés antibactériennes contre des souches de bactéries pathogènes. Des volumes d’hydrogel d’alginate à base de calcium, cuivre et zinc ont été aussi élaborés pour des applications dans le monde marin. La stabilité des différents matériaux a été étudiée dans différents milieux et à différentes températures. La propriété antimicrobienne de ces hydrogels a été démontrée sur deux souches de microalgues ainsi que sur quatre souches de bactéries marines sans toxicité. Finalement, le retardement de la formation de biofilms sur la surface d’acier inoxydable a été étudié par la méthode d’OCP en présence de matériaux à base de zincBiofouling is a phenomenon that affects every surface that is immersed in an aquatic medium covering it by a biofilm either in the medical or marine field. In order to prevent this phenomenon, in this work, biosourced alginate materials were elaborated. For applications in the medical field, hydrogel films of smooth or porous surface and films coating stainless steel by electrophoresis were elaborated. The presence of copper and zinc in their ionic form Cu2+ and Zn2+ was demonstrated.All the copper and zinc based films showed antibacterial properties on pathogenic bacterial strains.Alginate hydrogels in bulk based on calcium, copper and zinc were also elaborated for applications in the marine field. Stability of the different materials was studied in different mediums and at different temperatures. The antimicrobial property of these hydrogels based on copper and zinc was demonstrated on two microalgae stains as well as on four marine bacteria strains with no sign of toxicity. Finally, the delay in the formation of biofilms on stainless steel surfaces was studied by the OCP method in the presence of zinc based materials
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Dussud, Claire. "Caractérisation des communautés microbiennes associées à la colonisation des déchets plastiques en mer." Thesis, Paris 6, 2017. http://www.theses.fr/2017PA066254/document.
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La prise de conscience récente de la menace qui pèse sur les océans, réceptacle final de la pollution plastique, a donné lieu à une effervescence dans le domaine scientifique. On estime que plus de 5,25 milliards de particules plastiques flottent dans les océans. Ces travaux de thèse s’inscrivent dans le cadre de cette préoccupation environnementale de premier ordre, en apportant de nouvelles connaissances sur le compartiment bactérien qui se développe sur les débris plastiques en mer, appelé « plastisphère ». L’analyse des prélèvements effectués pendant l’expédition Tara-Méditerranée a permis de caractériser, pour la première fois dans cette zone, un biofilm abondant et spécifique des plastiques par comparaison aux communautés bactériennes attachées aux particules organiques ou libres dans l’eau de mer. Ensuite, la cinétique de colonisation bactérienne sur différents polymères a été étudiée grâce à la mise en place de microcosmes en circulation ouverte sur le milieu naturel. Le couplage original de données biologiques et physico-chimiques des surfaces plastiques a permis de constater un développement bactérien plus important sur des plastiques « biodégradables » (notamment des espèces hydrocarbonoclastes) par rapport aux polymères conventionnels. Enfin, de fortes activités hétérotrophes et ectoenzymatiques ont été constatées sur les polymères par rapport à l’eau de mer. Encore une fois, des différences en fonction des types de plastiques et du stade de formation du biofilm ont été observées. Les travaux menés pendant cette thèse mettent en lumière l’existence d’une nouvelle niche écologique sur les plastiques, distincte de celle de l’eau de mer environnanteThe increasing awareness on the impact of plastic pollution within the marine environment has stimulated countless of scientific studies. For the past decade, researchers have quantified plastic waste and assessed its fate at sea. It is estimated that more than 5.25 billion plastic particles float within the world’s oceans today. This PhD work is a result in part of this major environmental concern. It brings with it new knowledge about the marine bacterial communities that develop on plastic debris, also termed as the "plastisphere". The analysis of samples taken from the Tara-Mediterranean expedition allowed us, for the first time, to characterize, and quantify communities specific towards plastic biofilms in comparison to the communities attached to organic matter in surrounding seawater. Bacterial colonization and its evolution on different types of polymers was studied using microcosm experiments with open seawater circulation. The unusual coupling of biological and physicochemical data of plastic surfaces revealed a greater bacterial development on "biodegradable" polymers compared to conventional polymer types (especially hydrocarbonoclastic species). We showed that the composition of the polymer, together with its hydrophobicity and roughness, influences the diversity of bacterial communities during the early colonization steps. Finally, a greater bacterial biofilm activity (e.g. heterotrophic productions) was observed on polymer surfaces compared to seawater. Once again, differences according to plastic types have been observed. This present work highlights the existence of a new ecological niche on plastics that are distinct from the surrounding seawater
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Viano, Yannick. "Recherche de molécules non-toxiques actives en antifouling à partir d'organismes marins de Méditerranée." Phd thesis, Université du Sud Toulon Var, 2010. http://tel.archives-ouvertes.fr/tel-00595753.
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Alors que les premières formes de vie sont apparues dans les océans il y a près de 4 milliards d'années, les premières espèces terrestres remontent seulement à 400 millions d'années. Malgré cela, les substances naturelles d'origine marine ne représentent que 10% de l'ensemble des molécules isolées à ce jour à partir d'organismes vivants. Ces composés chimiques sont pourtant très spécifiques du fait des particularités du Monde Marin. Au sein des organismes qui les biosynthétisent, ils peuvent notamment intervenir dans la défense chimique contre les parasites et les autres compétiteurs. Ces molécules apparaissent donc comme des alternatives potentielles aux oxydes du tributylétain (TBTO) présents dans les revêtements antifouling et interdits depuis 2008 en raison de leur toxicité. Dans ce contexte et dans le cadre d'un partenariat avec le Parc national de Port-Cros, l'étude de la composition chimique de plusieurs organismes marins méditerranéens, des algues vertes (Caulerpa taxifolia) et brunes (Dictyota sp., Dictyota dichotoma, Cystoseira foeniculacea) ainsi que des bryozoaires, a été entreprise. Ces travaux ont permis l'isolement et la caractérisation structurale d'une vingtaine de métabolites secondaires (dérivés terpéniques et lipidiques, stérols), parmi lesquels huit sont originaux. L'identification de l'ensemble de ces molécules a été réalisée par le biais de l'utilisation de différentes méthodes spectroscopiques (RMN 1D et 2D, SM-HR) et la stéréochimie de certaines d'entre-elles a été établie par RMN (expérience NOESY 1H-1H) et modélisation moléculaire. Des corrections de données spectrales ont été également proposées pour cinq composés connus. Par ailleurs, l'activité anti-adhésion de plusieurs métabolites issus d'algues a été évaluée vis-à-vis d'un biofilm bactérien marin (Pseudoalteromonas sp.) afin de déterminer leur potentiel en tant qu'agents antifouling : plusieurs de ces composés présente une activité intéressante (CE50 = 30 μM), certes inférieure à celle du TBTO (CE50 = 10 μM), mais largement supérieure à celles de co-biocides utilisés actuellement. Parallèlement à ces travaux, l'analyse des variations temporelles et spatiales de l'expression métabolique de certaines des espèces étudiées a été réalisée à des fins écologiques. D'une part, les extraits mensuels de C. taxifolia présentent généralement une activité anti-adhésion importante quand le taux de caulerpényne dans l'extrait est élevé. D'autre part, d'importantes fluctuations ont notamment été observées dans la composition chimique des bryozoaires Myriapora truncata et Pentapora fascialis en fonction de leur lieu de récolte.
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Viano, Yannick. "Recherche de molécules non-toxiques actives en antifouling à partir d'organismes marins de Méditerranée." Toulon, 2010. https://theses.hal.science/tel-00595753/fr/.
Full textAbstract:
Alors que les premières formes de vie sont apparues dans les océans il y a près de 4 milliards d'années, les premières espèces terrestres remontent seulement à 400 millions d'années. Malgré cela, les substances naturelles d'origine marine ne représentent que 10% de l'ensemble des molécules isolées à ce jour à partir d'organismes vivants. Ces composés chimiques sont pourtant très spécifiques du fait des particularités du Monde Marin. Au sein des organismes qui les biosynthétisent, ils peuvent notamment intervenir dans la défense chimique contre les parasites et les autres compétiteurs. Ces molécules apparaissent donc comme des alternatives potentielles aux oxydes du tributylétain (TBTO) présents dans les revêtements antifouling et interdits depuis 2008 en raison de leur toxicité. Dans ce contexte et dans le cadre d'un partenariat avec le Parc national de Port-Cros, l'étude de la composition chimique de plusieurs organismes marins méditerranéens, des algues vertes (Caulerpa taxifolia) et brunes (Dictyota sp. , Dictyota dichotoma, Cystoseira foeniculacea) ainsi que des bryozoaires, a été entreprise. Ces travaux ont permis l'isolement et la caractérisation structurale d'une vingtaine de métabolites secondaires (dérivés terpéniques et lipidiques, stérols), parmi lesquels huit sont originaux. L'identification de l'ensemble de ces molécules a été réalisée par le biais de l'utilisation de différentes méthodes spectroscopiques (RMN 1D et 2D, SM-HR) et la stéréochimie de certaines d'entre-elles a été établie par RMN (expérience NOESY 1H-1H) et modélisation moléculaire. Des corrections de données spectrales ont été également proposées pour cinq composés connus. Par ailleurs, l'activité anti-adhésion de plusieurs métabolites issus d'algues a été évaluée vis-à-vis d'un biofilm bactérien marin (Pseudoalteromonas sp. ) afin de déterminer leur potentiel en tant qu'agents antifouling : plusieurs de ces composés présente une activité intéressante (CE50 = 30 μM), certes inférieure à celle du TBTO (CE50 = 10 μM), mais largement supérieure à celles de co-biocides utilisés actuellement. Parallèlement à ces travaux, l'analyse des variations temporelles et spatiales de l'expression métabolique de certaines des espèces étudiées a été réalisée à des fins écologiques. D'une part, les extraits mensuels de C. Taxifolia présentent généralement une activité anti-adhésion importante quand le taux de caulerpényne dans l'extrait est élevé. D'autre part, d'importantes fluctuations ont notamment été observées dans la composition chimique des bryozoaires Myriapora truncata et Pentapora fascialis en fonction de leur lieu de récolteLess than 10% of all natural products allready described in literature have a marine origin. However, these metabolites are often very specific due to the physicochemical and ecological properties of a such environment. In an ecological point of view, marine secondary metabolites are knowmn to be implicate in the defense against competitors. Thus, these compounds could be considered as potential alternatives to tributyltin derivatives (TBTO) outlawed in 2008 in antifouling coatings due to their high toxicity against non-targeted organisms. In this contexte, the phytochemical investigation of green (Caulerpa taxifolia) and brown algae (Dictyota sp. , D. Dichotoma, Cystoseira foeniculacea) was performed in partnership with the National Park of Port-Cros. This work led to the isolation of 23 metabolites (terpenes, sterols and lipids), from which 8 were found to be new.
Books on the topic "Biofilms marins"
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1928-, Fingerman Milton, Nagabhushanam Rachakonda, and Thompson Mary-Frances, eds. Biofilms, bioadhesion, corrosion, and biofouling. Enfield, NH: Science Publishers, 1999.
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Cheung, Chin Wa Sunny. Biofilms of marine sulphate-reducing bacteria on mild steel. Portsmouth: University of Portsmouth, Division of Chemistry, 1995.
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Patel, Pratixa. Interaction between marine biofilms and the zoospores of the green macrofouling alga Enteromorpha. Birmingham: University of Birmingham, 2003.
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Tapper, Rudi. The use of biocides for the control of marine biofilms on stainless steel surfaces. Portsmouth: University of Portsmouth, School of Pharmacy and Biomedical Sciences, 1998.
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1943-, Fusetani Nobuhiro, and Clare Anthony S, eds. Antifouling compounds. Berlin: Springer, 2006.
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(Editor), Milton Fingerman, Rachakonda Nagabhushanam (Editor), and Mary-Frances Thompson (Editor), eds. Recent Advances in Marine Biotechnology: Biofilms, Bioadhesion, Corrosion, and Biofouling (Recent Advances in Marine Biotechnology). Science Publishers, 1999.
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Fingerman, Milton. Recent Advances in Marine Biotechnology: Biofilms, Bioadhesion, Corrosion, and Biofouling. Taylor & Francis Group, 2020.
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Fingerman, Milton. Recent Advances in Marine Biotechnology: Biofilms, Bioadhesion, Corrosion, and Biofouling. Taylor & Francis Group, 2020.
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Fingerman, Milton. Recent Advances in Marine Biotechnology: Biofilms, Bioadhesion, Corrosion, and Biofouling. Taylor & Francis Group, 2020.
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Fingerman, Milton. Recent Advances in Marine Biotechnology: Biofilms, Bioadhesion, Corrosion, and Biofouling. Taylor & Francis Group, 2020.
Find full textBook chapters on the topic "Biofilms marins"
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Hossain, Md Foysul, Jakir Hossain, and Roksana Jahan. "Application of Marine Biofilms." In Microbial Biofilms, 219–51. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003184942-14.
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Stolz, John F. "Structure of Marine Biofilms." In Fossil and Recent Biofilms, 65–76. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0193-8_4.
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Mitra, Sayani, Barindra Sana, and Joydeep Mukherjee. "Ecological Roles and Biotechnological Applications of Marine and Intertidal Microbial Biofilms." In Productive Biofilms, 163–205. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/10_2014_271.
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Reid, Pamela, C. D. Dupraz, P. T. Visscher, and D. Y. Sumner. "Microbial Processes Forming Marine Stromatolites." In Fossil and Recent Biofilms, 103–18. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0193-8_6.
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Stal, Lucas J. "Nitrogen Cycling in Marine Cyanobacterial Mats." In Fossil and Recent Biofilms, 119–40. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0193-8_7.
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Kuroda, Daisuke. "Ships and Marine Structures." In Biofilm and Materials Science, 85–91. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-14565-5_11.
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Reimers, Clare E. "Applications of Bioelectrochemical Energy Harvesting in the Marine Environment." In Biofilms in Bioelectrochemical Systems, 345–66. Hoboken, NJ, USA: John Wiley & Sons, Inc, 2015. http://dx.doi.org/10.1002/9781119097426.ch10.
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Decho, Alan W., and Tomohiro Kawaguchi. "Extracellular Polymers (EPS) and Calcification within Modern Marine Stromatolites." In Fossil and Recent Biofilms, 227–40. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0193-8_14.
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Gillan, David C. "The Study of a Recent Iron-Encrusted Biofilm in the Marine Environment." In Fossil and Recent Biofilms, 241–48. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0193-8_15.
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Wiencek, K. M., and Madilyn Fletcher. "Effects of Substratum Hydrophobicity and Steric Hindrance on Adhesion of a Marine Pseudomonas SP." In Biofilms — Science and Technology, 99–104. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-1824-8_10.
Full textConference papers on the topic "Biofilms marins"
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Mahmoud, Mohamed A., and Ahmed H. Al-Salman. "Prediction of Microbial Induced Bacteria Using Innovative Semisolid Growth Media for General Aerobic Bacteria at Specific PH." In International Petroleum Technology Conference. IPTC, 2024. http://dx.doi.org/10.2523/iptc-24637-ms.
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ABSTRACT Structural steel is widely used in marine environments because it is strong, readily available, easy to fabricate, and cost-effective. But steel is also subject to corrosion. Microbiologically influenced corrosion (MIC) plays a critical role in the pipeline corrosion process, caused by electrochemical reactions created by microorganisms that form ‘biofilms’ on immersed steel structures. The close monitoring of microbial growth is an essential process to protect the structural steel from biofilm formation. A semisolid growth media for quantitative and qualitative analysis of general aerobic bacteria (GAB) was studied and counterchecked with commercially available ready-made media Paddle tester double-sided slides, to prove the suitability to use the Total Coliform agar media with a two-day incubation at 35°C, for quantitative determination of GAB colonies at a specific pH range from 5.5 to 7.5. Several trials were conducted, including water streams at upstream facilities, rich with GAB and Coliform sources, and all the obtained results from both media were matched.
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Abdoli, Leila, Yi Liu, Xiaoyan He, and Hua Li. "Bacillus sp.–Triggered Biocorrosion of Arc Sprayed Aluminum Coatings in Artificial Seawater." In ITSC2018, edited by F. Azarmi, K. Balani, H. Li, T. Eden, K. Shinoda, T. Hussain, F. L. Toma, Y. C. Lau, and J. Veilleux. ASM International, 2018. http://dx.doi.org/10.31399/asm.cp.itsc2018p0716.
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Abstract Thermal sprayed marine coatings in the marine environment usually encounter chloride-induced corrosion and microbiologically induced corrosion. Formation of microbial biofilm is crucial for subsequent attachment of large fouler and understanding the initiation and growth of the biofilm is essential for possibly controlling the occurring of biofouling. This paper reports the formation of Bacillus sp. bacterial biofilm on arc sprayed aluminum coatings and its effect on the corrosion behaviors of the coatings. Results show fast and pronounced attachment and colonization of the bacteria on aluminum coatings. The bacterial biofilm was systematically examined by CLSM, FESEM, and Raman spectroscopy. Electrochemical assessment revealed that the aluminum coating immersed in the bacteria-containing media showed higher corrosion resistance than the sterile samples. A model was proposed to explain how the microorganisms and their metabolic by-products protect the coatings against penetration of corrosive media. The results would give insight into design and fabrication of thermal sprayed coatings for enhanced anti-biocorrosion performances in the marine environment.
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Kanematsu, H., T. Kogo, D. Kuroda, H. Itoh, and S. Kirihara. "Biofilm Formation and Evaluation for Spray Coated Metal Films on Laboratory Scale." In ITSC2013, edited by R. S. Lima, A. Agarwal, M. M. Hyland, Y. C. Lau, G. Mauer, A. McDonald, and F. L. Toma. ASM International, 2013. http://dx.doi.org/10.31399/asm.cp.itsc2013p0520.
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Abstract The aim of this study is to determine what types of spray coated metals are desirable for inhibiting iron dissolution in marine environments as a countermeasure against biofilm formation. Experiments were conducted in a closed-loop system in which water continually circulates from a basal water tank through a transparent column where test specimens are immersed. The water flows through an open-air channel on its return to the tank where it is exposed to ambient bacteria. Steel and stainless steel substrates were prepared by plasma and HVOF spraying and, in some cases, a silicon-based sealant was applied. Test specimens were placed in the biofilm reactor and taken out and examined after 5 to 10 days. Substrate surfaces and corrosion products were analyzed by optical microscopy, x-ray diffraction, 3D surface profiling, and low-pressure SEM. Detailed results are presented and discussed in the paper.
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Liu, Yi, Xiaoqi Shao, Jing Huang, and Hua Li. "Flame Sprayed Environmentally Friendly High Density Polyethylene (PE) and Capsaicin Composite Coatings for Marine Antifouling Applications." In ITSC2018, edited by F. Azarmi, K. Balani, H. Li, T. Eden, K. Shinoda, T. Hussain, F. L. Toma, Y. C. Lau, and J. Veilleux. ASM International, 2018. http://dx.doi.org/10.31399/asm.cp.itsc2018p0732.
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Abstract Marine biofouling has emerged as worldwide serious problems for artificial marine infrastructures. Among the measures taken so far to solve such problems, construction of an antifouling layer has been proven to be effective in offering long-term antifouling performances. Antifouling based on the use of biocides is the most important method in modern maritime industries. While tributyltin (TBT)-based self-polishing coatings are being replaced by other biocide-releasing coatings, the environmental toxicity of these compounds is also under scrutiny. Therefore, there is a significant interest in developing non-toxic technologies. Green biocides can also be extracted from many types of organisms including terrestrial plants, sea creatures and bacteria. In this study, flame sprayed polyethylene (PE)-capsaicin composite coatings were developed for marine antifouling applications. Capsaicin powder were fixed by polymer-based substrate and distributed evenly. Antifouling test indicated excellent antibacterial properties of PE-capsaicin composite coatings against adhesion of marine Bacillus sp. bacteria. Prohibited formation of biofilm on the surfaces of the thermal sprayed composite coatings gives clear insight into their potential applications as antifouling layers in the marine environment.
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Yang, Jia-Dong, and Feng-Ling Xu. "MARINE CORROSION BEHAVIOR OF THE COPPER ALLOY B10 UNDER SULFATE-REDUCING BACTERIA BIOFILM." In 2015 International Conference on Material Engineering and Mechanical Engineering (MEME2015). WORLD SCIENTIFIC, 2016. http://dx.doi.org/10.1142/9789814759687_0143.
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Droumpali, Ariadni, Jörg Hübner, Lone Gram, and Rafael Taboryski. "Fabrication of micro-structured surface topologies for the promotion of marine bacteria biofilm." In Micromachines 2021 — 1st International Conference on Micromachines and Applications (ICMA2021). Basel, Switzerland: MDPI, 2021. http://dx.doi.org/10.3390/micromachines2021-09579.
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Serrano-Aroca, Ángel, Belén Frígols, Miguel Martí, Sofía Ingresa-Capaccioni, and Victoria Moreno-Manzano. "Prácticas de laboratorio interdisciplinares de alto nivel científico con alumnos de diferentes grados universitarios guiados por WebQuest AICLE." In IN-RED 2019: V Congreso de Innovación Educativa y Docencia en Red. València: Editorial Universitat Politècnica de València, 2019. http://dx.doi.org/10.4995/inred2019.2019.10365.
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Cada vez resulta más importante la colaboración entre expertos de diferentes áreas científicas multidisciplinares. En este trabajo, se han realizado prácticas de laboratorio agrupando alumnos de cuatro grados universitarios del área de biomedicina: Biotecnología, Ciencias del Mar, Veterinaria, Odontología y un grado impartido en inglés: Dentistry. Las asignaturas, que participaron en el estudio fueron: Biorreactores, Cultivos Celulares, Microbiología Marina, Microbiología Veterinaria, Microbiología de Odontología y Microbiology de Dentistry. Se abordó el tema de las síntesis química y por impresión 3D de biomateriales, su caracterización antimicrobiana por tres métodos complementarios (difusión en agar, contacto y formación de biofilm en biorreactor) y repoblación por cultivo con células madre adultas. Se diseñó una WebQuest con las instrucciones, laboratorio virtual y guías de prácticas en formato digital. Con motivo de llevar a cabo un Aprendizaje Integrado de Contenido y de Lenguas Extranjeras (AICLE), la WebQuest fue diseñada en inglés y los participantes realizaron una exposición en inglés al finalizar la experiencia. Las prácticas fueron realizadas en los laboratorios de la Universidad Católica de Valencia y en el Centro de Investigación Príncipe Felipe. Este procedimiento fue evaluado mediante un cuestionario de 14 preguntas, y mediante dos rúbricas para las memorias y exposiciones.
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Pogorzelski, S. J., and A. Szczepanska. "On-line and in-situ kinetics studies of biofilm formation on solid marine submerged substrata by contact angle wettability and microscopic techniques." In 2014 IEEE/OES Baltic International Symposium (BALTIC). IEEE, 2014. http://dx.doi.org/10.1109/baltic.2014.6887866.
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Oliveira, Vitoria Argento Huelsen de, ANA CLARA DE QUEIRÓZ, CAROLAYNE SANTINO, INGRID GABRIELA DE ALMEIDA, and ISABELA RODRIGUES ESCOBAR. "COMPARATIVO DA RIQUEZA DE ALGAS DO CAMPUS LAGOA DO SINO, BURI, SÃO PAULO." In II Congresso Brasileiro de Biodiversidade Virtual. Revista Multidisciplinar de Educação e meio ambiente, 2022. http://dx.doi.org/10.51189/ii-conbiv/7154.
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Introdução: Quando se tratando de macroalgas e algas de biofilme, ainda sabe-se pouco e é um tema pouco estudado. Objetivo: Visando a caracterização das espécies de macroalgas presentes no campus Lagoa do Sino, da universidade federal de São Carlos, esse trabalho teve por objetivo analisar quais algas se desenvolviam na região, levando em consideração a teoria do rio contínuo e fatores ambientais. Material e métodos: Dessa forma, para padronização experimental utilizou-se uma superfície de garrafa PET lixada com lixas de construção, de forma a aumentar a aderência e melhorar a fixação vegetal. Colocou-se 24 pedaços de garrafa, e foram distribuídos os pedaços em três trechos de dois riachos (cabeceira, meio e desembocadura), com dois fragmentos em cada trecho, sendo que foram colocados um pedaço próximo a cada uma das margens, os riachos foram chamados de riacho 1 e riacho 2, para facilitar a identificação. Após a coleta utilizou-se o laboratório da universidade, e com o auxílio de lupas, pinças e uma chave de identificação de macroalgas foram feitas as identificações das espécies. Resultados: O que se esperava segundo a literatura era encontrar uma média de sete a oito espécies, ou seja, pelo menos quatorze em cada riacho, mas obteve-se uma média de três espécies de macroalgas. Era esperado também que o riacho 1 tivesse maior riqueza, uma vez que o ambiente é mais iluminado e não sofre interferência de agrotóxicos. Conclusão: Ao final viu-se que, contrariando a teoria do rio contínuo, de modo à encontrar as mesmas espécies ao longo do rio todo, quando comparando os três trechos escolhidos, e também viu-se que o riacho 2 que possuía mais sombra e influência de agrotóxicos possuía maior riqueza de espécies (cinco espécies de macroalgas) quando comparado com o riacho 1 (três espécies de macroalgas).
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Pompermaier, Carolina, Willian Ely Pin, Mateus Xavier Schenato, Tales Antunes Franzini, and Guilherme Roloff Cardoso. "BREAST IMPLANT-ASSOCIATED ANAPLASTIC LARGE CELL LYMPHOMA: A LITERATURE REVIEW." In XXIV Congresso Brasileiro de Mastologia. Mastology, 2022. http://dx.doi.org/10.29289/259453942022v32s1012.
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Objective: This review aims to bring updates about the relationship between the silicone implant and the breast implantassociated anaplastic large cell lymphoma (BIA-LCL), in order to have a better knowledge about this disease. Despite the low risk of its development, a better understanding of BIA-ALCL is of interest to women, oncologists, breast specialists, plastic surgeons, regulatory agencies, and the general public, as the number of women with breast implants is increasing worldwide. Methods: This article is based on a review of publications on the topic. A search for articles was carried out through the SciELO databases, at the interface of the U.S. National Library of Medicine and National Center for Biotechnology Information (PubMed) and Latin American and Caribbean Literature on Health Sciences (LILACS). Results: BIA-ALCL is a very rare disease (1 case per 1–3 million women with implants), accounting for 2–3% of these lymphomas in adults and 0.5% of breast cancers and occurs between 8 and 10 years after breast cancer and implantation of a breast prosthesis. Textured implants are the most associated because they have a greater contact surface, so more biofilm is formed, causing bacterial adhesion. Most patients have peri-implant effusion and less often have a mass. Other described symptoms included breast enlargement, skin rash, capsular contracture, and lymphadenopathy. Lymphoma may be located in the seroma cavity or may involve pericapsular fibrous tissue. To make the diagnosis, imaging tests and cytological analysis must be performed. The fluid must be aspirated and is usually cloudy and thick, with large pleomorphic epithelioid lymphocytes, abundant cytoplasm, eccentric reniform nucleus and prominent nucleolus, and anaplastic lymphoma (ALK). Morphological and immunophenotypic features are indistinguishable from those of ALK-negative ALCL. Conclusion: The treatment of BIA-LCL includes implant removal, complete capsulectomy, excision of suspected adenopathy, and excision of lymphoma margins. Surgeons may consider removal of the contralateral implant as approximately 4.6% of cases have demonstrated incidental lymphoma in the contralateral breast. There are no data to recommend a mastectomy, sentinel lymph node biopsy, axillary lymphadenectomy, or breast reconstruction. The best prognosis is with complete capsule elimination surgery. Follow-up is done every 3–6 months for 2 years, in addition to imaging tests and the segment will depend on the patient’s clinical manifestations.
Reports on the topic "Biofilms marins"
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Wurl, Oliver. Biofilm-like habitat at the sea-surface: A mesocosm study, Cruise No. POS537, 14.09.2019 – 04.10.2019, Malaga (Spain) – Cartagena (Spain) - BIOFILM. University of Oldenburg, November 2020. http://dx.doi.org/10.3289/cr_pos537.
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OceanRep OceanRep Startseite Kontakt Schnellsuche Einfache Suche Erweiterte Suche Blättern Autor Forschungsbereich Publikationsart Jahr Studiengang Neuzugänge Artikel – begutachtet Alle Über uns GEOMAR Bibliothek Open Access Policies Grundsätze Hilfe FAQs Statistik Impressum Biofilm-like habitat at the sea-surface: A mesocosm study, Cruise No. POS537, 14.09.2019 – 04.10.2019, Malaga (Spain) – Cartagena (Spain) - BIOFILM . Logged in as Heidi Düpow Einträge verwaltenManage recordsManage shelvesProfilGespeicherte SuchenBegutachtungAdminLogout - Tools Wurl, Oliver, Mustaffa, Nur Ili Hamizah, Robinson, Tiera-Brandy, Hoppe, Jennifer, Jaeger, Leonie, Striebel, Maren, Heinrichs, Anna-Lena, Hennings, Laura Margarethe, Goncalves, Rodrigo, Ruiz Gazulla, Carlota und Ferrera, Isabel (2020) Biofilm-like habitat at the sea-surface: A mesocosm study, Cruise No. POS537, 14.09.2019 – 04.10.2019, Malaga (Spain) – Cartagena (Spain) - BIOFILM . Open Access . POSEIDON Berichte . University of Oldenburg, Oldenburg, 35 pp. [img] Text Cruise_Reports_POS537_final.pdf - publizierte Version Available under License Creative Commons: Attribution 4.0. Download (2417Kb) | Vorschau Abstract Biofilm-like properties can form on sea surfaces, but an understanding of the underlying processes leading to the development of these biofilms is not available. We used approaches to study the development of biofilm-like properties at the sea surface, i.e. the number, abundance and diversity of bacterial communities and phytoplankton, the accumulation of gel-like particles and dissolved tracers. During the expedition POS537 we used newly developed and free drifting mesocosms and performed incubation experiments. With these approaches we aim to investigate the role of light and UV radiation as well as the microbes themselves, which lead to the formation of biofilms. With unique microbial interactions and photochemical reactions, sea surface biofilms could be biochemical reactors with significant implications for ocean and climate research, e.g. with respect to the marine carbon cycle, diversity of organisms and oceanatmosphere interactions.