Journal articles on the topic 'Bioarchive'
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Giet, O. E., M. A. Barata, K. J. Kryston, J. Araujo, M. Wilke-Douglas, and E. Baudoux. "Management of a disaster plan: particular issue of the bioarchive® cryogenic tank." Cytotherapy 15, no. 4 (April 2013): S29. http://dx.doi.org/10.1016/j.jcyt.2013.01.110.
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Sasayama, N., S. Wada, M. Sugoh, K. Saitoh, Y. Xu, S. Asano, and T. A. Takahashi. "Cryopreservation of placental/umbilical cord blood in an automated controlled-rate freezing and storage system, The "BioArchive System"." Journal of the Japan Society of Blood Transfusion 47, no. 1 (2001): 15–21. http://dx.doi.org/10.3925/jjtc1958.47.15.
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Atik, Nur, S. Putri Pratiwi, and Laniyati Hamijoyo. "Correlation between C-reactive Protein with Malondialdehyde in Systemic Lupus Erythematosus Patients." International Journal of Rheumatology 2020 (July 1, 2020): 1–5. http://dx.doi.org/10.1155/2020/8078412.
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Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by an inflammatory process. One of the inflammation markers that can be measured is C-reactive protein (CRP). Another indicator of inflammation is malondialdehyde (MDA), though it is still uncommon to be analyzed in SLE patients. The study looked for the MDA value and found a correlation with CRP. A cross-sectional study design with a correlative analytical was performed. CRP level data was taken from Hasan Sadikin lupus registry data, and MDA levels were analyzed from a bioarchive patient’s serum. We collected the patients’ data who had CRP level from Hasan Sadikin lupus registry and analysed MDA levels from the serum sample. MDA levels were analyzed using an ELISA method. The data obtained were analyzed using the Pearson correlation and Eta correlation test. The study involved 78 data patients as subjects. It was found that the median of CRP and MDA was 0.85 mg/l and 153.10 ng/ml, respectively. These results indicate that the CRP levels in SLE patients are still within normal limits. Statistical analysis showed no correlation between CRP and MDA level (r=0.2, P>0.05). Additionally, the correlation between CRP and MDA with organ involvement, such as lupus nephritis (LN), lupus cutaneous (LC), and lupus musculoskeletal (LM), showed no correlation (Fh<Ft). There is no correlation between CRP and MDA levels in SLE patients, and specific organ involvement of the disease does not affect the correlation.
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Quéma, Anne. "Bioarchives of Affect: Erín Moure’s The Unmemntioable." Special Issue: Neoliberal Environments 45, no. 2 (August 17, 2021): 25–47. http://dx.doi.org/10.7202/1080272ar.
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Shoulars, Kevin, Tracy Gentry, Pamela Noldner, Kristin M. Page, and Joanne Kurtzberg. "Cord Blood Unit Cryopreservation: Positioning Segments for Potency Assessment." Blood 126, no. 23 (December 3, 2015): 4309. http://dx.doi.org/10.1182/blood.v126.23.4309.4309.
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Abstract Introduction: Banked, unrelated umbilical cord blood units (CBU) are a rich source of hematopoietic stem cells for hematopoietic reconstitution. Potency assays on attached segments have been used for CBU release from the bank to the transplant center. CBUs have historically been manufactured and cryopreserved in bags with three segments (made from the tubing used to fill the bag) arranged across the top of the cassette. Prior to cryopreservation, the CBU is overwrapped, placed in a metal cassette and frozen in a control-rate freezer and submerged under liquid nitrogen for long-term storage. After cryopreservation, segments can be removed from the cassette, without disturbing the bag, for HLA typing, potency assays, or other testing. Last year, in an effort to increase the number of segments available for testing, we changed the configuration of the cryobag to create a fourth segment. To fit in the cassette, the segments were arranged with two segments across the top and two segments bent at 90 degrees to fit between the 20% and 80% portions of the bag. When performing our annual stability testing, we realized that the two distal segments, positioned between the 20% and 80% compartments yielded lower potency results than the segments positioned across the top of the cassette. We investigated this finding as described below. Method: Three large fresh CBUs were processed separately on a Sepax cell processor (Biosafe, Geneva, Switzerland) and evenly divided into two cryopreservation bags. For each CBU, one bag was created with three segments and the other bag with four segments. The cryopreservative, dimethylsulfoxide in dextran, was added at a final concentration of 10% to both bags prior to cryopreservation. Segments were positioned either across the top (3 segments) or in the bent configuration (4 segments) for each of the paired CBUs. All units were cryopreserved and stored in a Thermogenesis Bioarchive. After two weeks in storage, the CBUs were tested. The bags and segments were assayed for ALDHbr, viable CD34, CD45, glycophorin A and viability (7-AAD) along with colony forming units (CFUs), the potency assay used by our bank. Results: The segments configured across the top of the bag correlated with results from the cryobag for ALDHbr (as a percent of viable CD45+, Table 1), CD34+ (as a percent of viable CD45+) and CFU. In contrast, segments placed between the 20% and 80% compartments were significantly lower than the bag for both ALDHbr (as a percent of viable CD45+) and CFU, although CD34+ (as a percent of viable CD45+) was equivalent. Conclusions: Segments attached to CBUs can predict potency of the CBU by various parameters. However, proper placement of the segments within the cryopreservation cassette must be performed to ensure that potency measured on the segments reflects potency measured on the cord blood unit. Disclosures No relevant conflicts of interest to declare.
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Maddens, Stephane, Eric Petitprost, Isabelle Bardey, Christian Lamy, Francine Garnache, Philippe Saas, Jacqueline Chabod, and Valerie Lapierre. "Quality Assurance Improvement during Accreditation Process of a French Cord Blood Bank: FACT No Fiction." Blood 106, no. 11 (November 16, 2005): 5284. http://dx.doi.org/10.1182/blood.v106.11.5284.5284.
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Abstract The Cord Blood Banks (CBB) wishing to be affiliated to NETCORD must demonstrate that they adopted the Fact-Netcord standards for collection, processing, quality control testing, storage, selection and release for their cord blood units (CBU). The best way to provide these assurances is to obtain FACT accreditation label. The Besancon Cord Blood Bank initiated this quality approach in January 2004 and was inspected on-site in June 2004.Initial internal audits revealed major deficiencies in specific Quality Management areas and the lack of an efficient documentation system together with adequate standard operating procedures and documented validation studies for each step of the process. Adequate labelling, conformity with good transport practices and post-transplantation clinical data recovery were also areas to be improved. Moreover, in order to clarify the different pathways of material, cell products, staff, or wastes, several building works modifications had to be undertaken. Several major improvements were performed during those six months of Fact accreditation project. The Assurance Quality Information System was significantly upgraded to control all the major elements of assurance quality (staff formation, material, premises, alarms, cleaning, records, and quality control testing) by implementing a computerised Oracle-based application. Morover, all documentation was implemented on an intranet network and is now easily accessible to all the staff wherever in the facility. In order to improve the volume of CBU, a weigh-and-mix machine used for blood donor harvesting procedure was tailored for specific CBU collection. To improve the conditions of transport we set up special containers with controlled temperature continuously monitored. Finally, major validation study was performed on a short period, aiming at demonstrating efficiency and reproducibility of the overall CBU process. In brief, eligible mothers, who give their informed consent, are accepted for CB donation after a medical interview. Collection is performed with the placenta in utero. The delay between collection and processing must not exceed 24hrs. CBU volumes are reduced using Sepax© (Biosafe, Switzerland) technology for long-term storage in a Bioarchive© (Thermogenesis, CA, USA). Quality controls include testing maternal blood samples for infectious markers (HIV, HCV, HBV, HTLV, syphilis) and the presence of IGg- and IgM-antibodies against toxoplasmosis, EBV and CMV on the day of birth and after a quarantine period of at least 60 days. No active infectious disease is mandatory. A baby’s clinical examination is performed at the same time point. Microbiological tests on the CBU must be negative. In addition, our CBB requires a minimal collection volume of 80 ml and a minimum of 2 x 106 CD34 cells for CBU qualification. We implemented a systematic cord blood haemoglobin analysis and tested systematically cord blood plasma for viral infection by genomic detection. Finally, our bank obtained its FACT accreditation on May 2nd 2005, validating our strategy. This achievement had a great impact on CBB staff, desiring to maintain and improve their know how. On June 30th 2005, 5045 CBU were registered at French marrow registry and 273 (5%) were transplanted, 142 (52%) in France and 131 (48%) in other countries. This high 5% transplantation rate may be due to the stringent initial quality controls requirements.
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de Cesare, Sylvia. "Les « bioarchives » comme modèles pour l’écologie et les sciences de l’environnement : une perspective épistémologique." Bulletin d’histoire et d’épistémologie des sciences de la vie Volume 26, no. 1 (2019): 77. http://dx.doi.org/10.3917/bhesv.261.0077.
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Nielsen, Jan Kresten, and Jesper Kresten Nielsen. "Geoducks (Panopea abrupta) as isotopic bioarchives of seasonality in the climate of British Columbia." Ecological Research 24, no. 5 (January 9, 2009): 987–95. http://dx.doi.org/10.1007/s11284-008-0571-4.
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Scaradavou, Andromachi, Maria S. Albano, Nela-Ludy Dobrila, Katharine Smith, Marissa N. Lubin, Joann Tonon, Dorothy Sung, Cladd Stevens, and Juliet N. Barker. "Analysis of Cord Blood Unit (CBU) Segment for CD34+ Cell Viability and Hematopoietic Progenitor Cell Content Correlates with the Post-Thaw CBU After Albumin-Dextran Reconstitution." Blood 120, no. 21 (November 16, 2012): 3023. http://dx.doi.org/10.1182/blood.v120.21.3023.3023.
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Abstract Abstract 3023 Background: The segment attached to the freezing bag is considered an important source of cells for testing the quality of a cryopreserved CBU prior to release for transplantation. CB banks test cells from the segment for CD34+ cell count, viability and colony forming units (CFU) as surrogates of potency of the frozen products. The NCBP reported results of 384 segments of CBU processed with the AutoExpress (AXP) system (Albano et al, ASH 2011). Average segment CD34+ viability was 96% (SD:+/− 3.3%) and correlation with the CBU pre-cryopreservation viable CD34+ counts (vCD34; R2:0.9) and CFU (R2:0.6) were excellent. Segment vCD34+ and CFU also correlated highly with each other (R2:0.69). As of 08/2012, 1056 segments from NCBP AXP CBU have been evaluated with average CD34+ cell viability of 96% (SD:+/− 3.1). However, how the segment results compare to those obtained from the CBU at the transplant center is not established. Methods: To evaluate whether the segment could predict the post-thaw CBU vCD34+ counts, viability and CFU at MSKCC, we compared the post-thaw results of 37 NCBP CBU, AXP-processed and stored in BioArchive freezers, shipped, and thawed at MSKCC, with the information from their respective segments tested at NCBP prior to CBU release and the pre-cryopreservation data. Segment CD34+ counts and viability were evaluated by flow cytometry and 7-AAD exclusion using a single platform and the ISHAGE gating strategy. Segment CFU were evaluated using the NCBP CFU high resolution digital imaging technology (Albano et al, ASH 2008). The segment viable cells/ul were used to estimate the total vCD34 and CFU for the respective CBU. At MSKCC, CBU underwent thaw and albumin reconstitution with 8-fold dilution (10% Dextran 40; 25% albumin) as previously reported (Barker et al, BBMT 2009;15(12):1596–602). Duplicate samples were evaluated by flow cytometry within two hours. Four color flow cytometry using a dual platform was performed to measure CD45+/CD34+/CD3+ cell counts; CD34+ cell viability was assessed using a modified ISHAGE strategy (Scaradavou et al, BBMT 2010;16(4):560–8). CFU assays were performed using 1×105cells plated in duplicate and growth was evaluated at 14 days. All CBU were part of double unit grafts. Results: Consistent with prior NCBP data, segment vCD34+ cell counts correlated well with segment CFU (R2:0.89, p< 0.01; N=21). Additionally, high correlation of vCD34+ cell counts and CFU were seen between the pre-cryopreservation CBU and the segment (p<0.01, Table). Importantly, despite the differences in testing laboratories and gating strategies, the number of vCD34+ cells in the CBU post-thaw correlated with the pre-cryopreservation vCD34+ counts, as well as those from the segment (Table). Average decrease in post-thaw CBU CD34+ cell viability compared to that of the segment was 1.4% (SD:+/− 3.7%, N: 37). Although statistically significant (p: 0.029), this difference was not clinically relevant: the range of change was -10% to +4.8% and the lowest CBU CD34+ viability was 86% (Figure). Post-thaw CBU total CFU and CFUGM did not correlate with pre-cryopreservation values (Table), probably reflecting high inter-laboratory assay variability. Segment CFU and CFUGM correlation with the post-thaw values was significant although the R2was weak. The ratio of post-thaw to segment vCD34 cells (median: 1.3; SD:+/− 0.47) indicated that the segment calculation may underestimate CBU cell content. The median ratio of post-thaw to segment colony-forming cells was 0.54 for total CFU (SD:+/− 0.63) and 0.5 for CFUGM (SD:+/− 0.96). The lower CFU than vCD34 ratio may be explained, in part, by the fact that 7-AAD detects dead cells but not apoptotic; apoptotic cells are counted as alive by flow cytometry but do not have functionality and do not generate CFU in culture. Conclusions: Our results indicate that testing of CBU segments can measure accurately the potency of the frozen CB products. Moreover, the results demonstrate that CBU quality can be maintained following albumin-dextran reconstitution. These findings reflect the cryopreservation procedures, freezing bags and reconstitution method described; whether they can be applied to other CB banks or transplant center laboratories requires further investigation. Disclosures: No relevant conflicts of interest to declare.
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Ponnurangam, A., M. Bau, M. Brenner, and A. Koschinsky. "Mussel shells of <i>Mytilus edulis</i> as bioarchives of the rare earth elements and yttrium distribution in seawater and the potential impact of pH and temperature on the partitioning behaviour." Biogeosciences Discussions 12, no. 17 (September 9, 2015): 14911–39. http://dx.doi.org/10.5194/bgd-12-14911-2015.
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Abstract. Mussel shells are potential bioarchives of proxies for changes of the physico-chemical conditions in the bivalve's habitat. One such proxy is the distribution of the Rare Earths and Yttrium (REY) in seawater, as REY speciation in seawater is sensitive to pH and temperature variations, due to the impact of these parameters on the activity of CO32− in seawater. We present a new protocol for sample preparation and determination of REY concentrations in bivalve shells, that includes sample treatment with NaOCl followed by REY separation and preconcentration. The data obtained was further used to calculate REY partition coefficients between shells of M. edulis and ambient seawater, and acquired results were then used in the investigation of the potential effects of pH and temperature on REY partitioning. Shells of M. edulis mussels from the North Sea show consistent shale-normalized ("SN") REY patterns that increase from the light REY to the middle REY and decrease from the middle REY to the heavy REY. Despite being different to the general seawater REYSN pattern, the shells still display distinct REY features of seawater such as a negative CeSN anomaly and small positive YSN and GdSN anomalies. Apparent partition coefficients for the REY between the shell and seawater (appDREYshell/seawater) are low and decrease strongly from the light REY (4.04 for La) to the heavy REY (0.34 for Lu). However, assuming that only the free REY3+ are incorporated into the shell, appDREY3&plus;shell/seawater values are higher and rather similar for all REY (102.46 for La; 113.44 for Lu), but show a slight maximum at Tb (199.18). Although the impact of vital effects i.e. REY speciation in a mussel's extrapallial fluid from which the carbonate minerals precipitate, cannot be quantified yet, it appears that M. edulis shells are bioarchives of some REY features of seawater. We modelled the REYSN patterns of a hypothetical mussel shell at pH 8.2 and 7.6 and at temperatures of 25 and 5 °C assuming that only REY3+ are incorporated into the carbonate's crystal lattice. The results suggest that with lower pH, REY concentrations in a shells increase, but with little effect on the shape of the REYSN patterns, while a temperature change has an impact on the REYSN pattern, but only minor effects on REY concentrations.
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Schneider, Heike. "Landscape and vegetation change on the Iberian Peninsula during the Roman Epoch – A reconstruction based on Geo-Bioarchives." Quaternary International 279-280 (November 2012): 436–37. http://dx.doi.org/10.1016/j.quaint.2012.08.1423.
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Ponnurangam, A., M. Bau, M. Brenner, and A. Koschinsky. "Mussel shells of <i>Mytilus edulis</i> as bioarchives of the distribution of rare earth elements and yttrium in seawater and the potential impact of pH and temperature on their partitioning behavior." Biogeosciences 13, no. 3 (February 11, 2016): 751–60. http://dx.doi.org/10.5194/bg-13-751-2016.
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Abstract. Mussel shells are potential bioarchives of proxies for changes in the physicochemical conditions in the bivalve's habitat. One such proxy is the distribution of rare earths and yttrium (REY) in seawater, as REY speciation in seawater is sensitive to pH and temperature variations, due to the impact of these parameters on the activity of CO32− in seawater. We present a new protocol for sample preparation and determination of ultratrace concentrations of REY in bulk bivalve shells (comprised of calcite and aragonite) that includes sample treatment with NaOCl followed by REY separation and preconcentration. The data obtained were used to calculate REY partition coefficients between bulk bimineralic shells of Mytilus edulis (calcite aragonite mix) and ambient seawater, and the results acquired were then used to investigate the potential effects of pH and temperature on REY partitioning.Shells of Mytilus edulis mussels from the North Sea show consistent shale-normalized (SN) REY patterns that increase from the light REY to the middle REY and decrease from the middle REY to the heavy REY. Despite being different from the general seawater REYSN pattern, the shells still display distinct REY features of seawater, such as a negative CeSN anomaly and small positive YSN and GdSN anomalies. Apparent REY partition coefficients between shells and seawater (appDTot.REYshell/seawater) are low and decrease strongly from the light REY (4.04 for La) to the heavy REY (0.34 for Lu). However, assuming that only the free REY3+ are incorporated into the shell, modDFreeREY3+shell/seawater values are higher and comparatively similar for all REY (102.46 for La; 113.44 for Lu) but show a slight maximum at Tb (199.18). Although the impact of vital effects, such as REY speciation in a mussel's extrapallial fluid from which the carbonate minerals precipitate, cannot be quantified yet, it appears that M. edulis shells are bioarchives of some REY features of seawater.We modeled the REYSN patterns of a hypothetical mussel shell at pH 8.2 and 7.6 and at temperatures of 25 and 5 °C, assuming that only free REY3+ are incorporated into the carbonate's crystal lattice and that vital effects do not obliterate the REY signal of the shells. The results suggest that with lower pH, REY concentrations in shells increase, but with little effect on the shape of the REYSN patterns, while a temperature change has an impact on the REYSN pattern but only minor effects on REY concentrations. Hence, after additional calibration studies, the REY systematics in mussel shells may become a valuable proxy for paleo-pH and ocean acidification.
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Pérez-Huerta, Alberto, Sally E. Walker, and Chiara Cappelli. "In Situ Geochemical Analysis of Organics in Growth Lines of Antarctic Scallop Shells: Implications for Sclerochronology." Minerals 10, no. 6 (June 10, 2020): 529. http://dx.doi.org/10.3390/min10060529.
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Bivalve shells are extensively used as bioarchives for paleoclimate and paleoenvironmental reconstructions. Proxy calibrations in recent shells are the basis for sclerochronology and the applications of geochemistry data to fossils. Shell geochemical information, however, could be altered with the disappearance of intercrystalline organic matrix components, including those linked to shell growth increments, during early diagenesis. Thus, an evaluation of the chemistry of such organics is needed for the correct use of sclerochronological records in fossil shells. Here, we use atom probe tomography (APT) for in situ geochemical characterization of the insoluble organic matrix in shell growth increments in the Antarctic scallop, Adamussium colbecki. We confirm the presence of carboxylated S-rich proteoglycans, possibly involved in calcite nucleation and growth in these scallops, with significant concentrations of magnesium and calcium. Diagenetic modification of these organic components could impact proxy data based on Mg/Ca ratios, but more importantly the use of the δ15N proxy, since most of the shell nitrogen is likely bound to the amide groups of proteins. Overall, our findings reinforce the idea that shell organics need to be accounted for in the understanding of geochemical proxies.
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Olivier, Frédéric, Blandine Gaillard, Julien Thébault, Tarik Meziane, Réjean Tremblay, Dany Dumont, Simon Bélanger, et al. "Shells of the bivalve Astarte moerchi give new evidence of a strong pelagic-benthic coupling shift occurring since the late 1970s in the North Water polynya." Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 378, no. 2181 (August 31, 2020): 20190353. http://dx.doi.org/10.1098/rsta.2019.0353.
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Climate changes in the Arctic may weaken the currently tight pelagic-benthic coupling. In response to decreasing sea ice cover, arctic marine systems are expected to shift from a ‘sea-ice algae–benthos' to a ‘phytoplankton-zooplankton’ dominance. We used mollusc shells as bioarchives and fatty acid trophic markers to estimate the effects of the reduction of sea ice cover on the food exported to the seafloor. Bathyal bivalve Astarte moerchi living at 600 m depth in northern Baffin Bay reveals a clear shift in growth variations and Ba/Ca ratios since the late 1970s, which we relate to a change in food availability. Tissue fatty acid compositions show that this species feeds mainly on microalgae exported from the euphotic zone to the seabed. We, therefore, suggest that changes in pelagic-benthic coupling are likely due either to local changes in sea ice dynamics, mediated through bottom-up regulation exerted by sea ice on phytoplankton production, or to a mismatch between phytoplankton bloom and zooplankton grazing due to phenological change. Both possibilities allow a more regular and increased transfer of food to the seabed. This article is part of the theme issue ‘The changing Arctic Ocean: consequences for biological communities, biogeochemical processes and ecosystem functioning'.
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Tavener, Selena K., Dennis E. Jewell, and Kiran S. Panickar. "The Increase in Circulating Levels of Pro-Inflammatory Chemokines, Cytokines, and Complement C5 in Canines with Impaired Kidney Function." Current Issues in Molecular Biology 44, no. 4 (April 11, 2022): 1664–76. http://dx.doi.org/10.3390/cimb44040114.
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Chronic low-grade inflammation is a key contributor to the progression of kidney disease. The release of cytokines and other pro-inflammatory proteins may further contribute to detrimental kidney health by increasing interstitial edema and renal fibrosis. The aim of the present study was to investigate the inflammatory markers in canines who developed renal disease naturally and were diagnosed with renal disease either during life or following necropsy, as assessed by a veterinarian. RNA was isolated from canine blood obtained at necropsy and stored as bioarchived samples from ten canines with renal disease (9.6–14.7 yr) and ten controls (10.1–14.8 yr). At the time of death, the mean blood creatinine concentration and BUN were elevated in dogs with renal disease compared to control (both p < 0.01). Samples were assessed for changes in gene expression using the Canine cytokine RT2 Profiler PCR Array for inflammation. There was a significant increase in C-C Motif Chemokine Ligand 16 (CCL16), C-X-C Motif Chemokine Ligand 5 (CXCL5), Interleukin 16 (IL-16), and Complement Component 5 (C5) (all p < 0.05 vs. con). In addition, there was also a statistically non-significant increase in 49 genes and a down-regulation in 35 genes from a panel of total 84 genes. Pro-inflammatory genes including CCL16, CXCL5, IL-16, and C5 can all contribute to renal inflammation and fibrosis through different signaling pathways and may lead to a progressive impairment of kidney function. Blockade of their activation may be important in ameliorating the initiation and/or the progression of renal disease.
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Bell, Alexander T., Kohei Fujikura, Jacob Stern, Rena Chan, James Chell, Stephen Williams, Ashley Kiemen, et al. "Abstract 637: Spatial transcriptomics for FFPE characterizes the molecular and cellular architecture of malignant changes in pancreatic pre-malignant lesions." Cancer Research 82, no. 12_Supplement (June 15, 2022): 637. http://dx.doi.org/10.1158/1538-7445.am2022-637.
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Abstract We have optimized an experimental and computational pipeline to adapt spatial transcriptomics (ST) approaches based upon the Visium (10x Genomics) technology to infer cellular composition and intercellular interactions of FFPE clinical specimens. We apply this technology to deliver an approach to examine pancreatic intraepithelial neoplasia (PanIN) to identify intrinsic and extrinsic mechanisms that are associated with the progression of these pre-malignant lesions to invasive carcinoma. Currently, most pancreatic cancers are diagnosed at an advanced stage that reflects in dismal survival rates and a better understanding of PanINs biology will provide valuable insights for early therapeutic interventions. Thus, we used PanINs as our model system to implement the FFPE ST workflow. Our workflow for FFPE ST analysis facilitates sectioning of small regions (5mm in diameter) from a paraffin block that are stained and imaged with H&E and concurrently measured for genome-wide transcriptional profiling. Subsequently, the image is used for automated cell annotation using an algorithm, CODA, trained to identify normal and neoplastic pancreatic cell types. CODA identified the normal pancreatic histological regions (ducts, acini, islets of Langerhans, stroma), as well as the neoplastic cells. This automated analysis enables isolation of specific spots for differential expression analysis to pinpoint the transcriptional changes that occur within neoplastic cells along ducts in PanIn and their changes between high-grade and low-grade lesions. The spatial gene expression analysis identified clusters that mapped to the cell types annotated by CODA and the marker genes of each cluster matched known markers for the correspondent cell type. Although PanINs are very small in size (< 1mm), we found specific clusters accurately mapped to these lesions in each sample. Overall, the spatial sequencing data presented enough depth and complexity to allow differential expression and pathway analysis. We observed a significant number of deregulated genes in PanINs compared to normal ducts. Some deregulated genes are known PanIN markers, but potential new markers were also identified. Moreover, the integration of CODA with gene expression changes enables us to verify that unique stromal regions annotated with CODA and associated with PanIns are in fact heterogeneous and formed by distinct cell subtypes. Altogether, our workflow combining automated cell annotation with STA from the same section provides a methodology to precisely examine the sample architecture while measuring heterogeneity at the transcriptional level. This combined approach can be applied to different FFPE tumor types to leverage the use of large bioarchives of samples not previously accessible to genome-wide spatial methods. Citation Format: Alexander T. Bell, Kohei Fujikura, Jacob Stern, Rena Chan, James Chell, Stephen Williams, Ashley Kiemen, Elizabeth M. Jaffee, Denis Wirtz, Laura D. Wood, Elana J. Fertig, Luciane T. Kagohara. Spatial transcriptomics for FFPE characterizes the molecular and cellular architecture of malignant changes in pancreatic pre-malignant lesions [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 637.
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"Company News." Asia-Pacific Biotech News 05, no. 14 (July 9, 2001): 291–301. http://dx.doi.org/10.1142/s0219030301000908.
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Cell Robotics' Medical Devices Well Received in China. US National Nutritional Foods Association and China's Sanjiu Pharmaceutical Establish Historic Partnership. Novacor LVAS Approved for Commercial Use in Japan. NOMOS and Lynmed to Jointly Distribute Medical Products in China and HK. Eli Lilly to Buy Out Ranbaxy's Stake. US-based Dragon Expands Business in Hong Kong and China. Australia's Global Doctor and US MedAire Announce Strategic Alliance. Taiwan's Gene Born Biotech Corp Adopts Thermogenesis's BioArchive System. Cepheid and BioSynTech Sign Distribution Agreement To Enter Life Sciences Research Market in Southeast Asia. Harbin Pharmaceutical Completes China's Largest Antibiotic Wastewater Recycling Project. Eli Lilly to Set Up State-of-the-Art Systems Biology R&D Center in Singapore. Varian Receives First Order for World's Most Powerful NMR Spectrometer from Japan.
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Jewell, Dennis, Regina Hollar, Selena Tavener, and Kiran Panickar. "Cats with Calcium Oxalate Stones Have Increased Bone Mineral While Cats with Renal Disease Have Reduced Lean (P09-004-19)." Current Developments in Nutrition 3, Supplement_1 (June 1, 2019). http://dx.doi.org/10.1093/cdn/nzz033.p09-004-19.
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Abstract Objectives Evaluate the effects of renal disease and calcium oxalate stone formation in cats. Methods 42 cats were evaluated from one year of age to end of life (21 spayed females and 21 neutered males). There were three groups evaluated: 12 calcium oxalate stone forming cats (CaOx), 11 cats with renal disease (RD) and 19 healthy kidney cats (H). Their condition was defined during life or at the time of death. After death both bioarchive samples and historical data were analyzed. Analysis of serum (calcium, urea, and creatinine), urine (specific gravity) and body composition (lean, fat, bone mineral and bone mineral density) was accomplished. Dual Energy X-ray analysis was used to measure body composition. The Proc Mixed and Proc GLM program of SAS (9.4) was used for statistical analysis. A p value less than or equal to 0.05 was used as a cutoff for statistical significance. Results At the time of death circulating symmetric dimethyl arginine (SDMA) concentration was 11.0a, 45.5c and 27.4b ug/dl for H, RD and CaOx respectively. Lean body mass was 3.5a, 2.8b and 3.4a kg (H, RD and CaOx respectively). Body lean was significantly correlated (P < 0.01) with bone mineral content and creatinine, however there was no lean by group interaction. Creatinine concentration was influenced by body lean, before accounting for lean change it was 1.0a, 6.0b and 3.0c mg/dl and after adjustment for body lean was 0.7a, 6.7b, and 2.9c mg/dl (H, RD and CaOx respectively). Bone mineral content was 125a, 133a, b and 140b grams (H, RD and CaOx respectively using lean as a covariant). There was a differential effect of age on circulating calcium and phosphorus concentration. As cats aged both the RD and CaOx groups had increasing calcium concentration with increasing age in comparison to H, The CaOx group had increased calcium and phosphorus when compared to both RD and H. Conclusions These data show that SDMA has the advantage of not being influenced by lean body mass (as contrasted to creatinine). The data also leads to the conclusion that nutritional intervention in support of oxalate stone forming cats should include support for appropriate calcium phosphorus homeostasis while nutritional support for cats with renal disease should include optimum support of lean body mass. Funding Sources This study was funded by Hill's Pet Nutrition, Inc.