Journal articles: 'Bio-scaffold'

1

Rohman, Géraldine, Credson Langueh, Salah Ramtani, Jean-Jacques Lataillade, Didier Lutomski, Karim Senni, and Sylvie Changotade. "The Use of Platelet-Rich Plasma to Promote Cell Recruitment into Low-Molecular-Weight Fucoidan-Functionalized Poly(Ester-Urea-Urethane) Scaffolds for Soft-Tissue Engineering." Polymers 11, no. 6 (June 9, 2019): 1016. http://dx.doi.org/10.3390/polym11061016.

Full text

Abstract:

Due to their elastomeric behavior, polyurethane-based scaffolds can find various applications in soft-tissue engineering. However, their relatively inert surface has to be modified in order to improve cell colonization and control cell fate. The present study focuses on porous biodegradable scaffolds based on poly(ester-urea-urethane), functionalized concomitantly to the scaffold elaboration with low-molecular-weight (LMW) fucoidan; and their bio-activation with platelet rich plasma (PRP) formulations with the aim to promote cell response. The LMW fucoidan-functionalization was obtained in a very homogeneous way, and was stable after the scaffold sterilization and incubation in phosphate-buffered saline. Biomolecules from PRP readily penetrated into the functionalized scaffold, leading to a biological frame on the pore walls. Preliminary in vitro assays were assessed to demonstrate the improvement of scaffold behavior towards cell response. The scaffold bio-activation drastically improved cell migration. Moreover, cells interacted with all pore sides into the bio-activated scaffold forming cell bridges across pores. Our work brought out an easy and versatile way of developing functionalized and bio-activated elastomeric poly(ester-urea-urethane) scaffolds with a better cell response.

APA, Harvard, Vancouver, ISO, and other styles

2

Chen, Xiao Feng, Ying Jun Wang, Na Ru Zhao, and Chun Rong Yang. "Investigation on the Biomimetic Scaffold for Bone Tissue Engineering Based on Bioglass-Collagen-Hyaluronic Acid-Phosphatidylserine." Key Engineering Materials 330-332 (February 2007): 939–42. http://dx.doi.org/10.4028/www.scientific.net/kem.330-332.939.

Full text

Abstract:

The new type of bone tissue engineering scaffold composed of the sol-gel derived bioactive glass particles, type I collagen, hyaluronic acid and phosphatidylserine were prepared through cross-linking and freeze-drying techniques. SEM observation indicated that the scaffold possessed a 3-D interconnected porous structure and a high porosity. The properties of bio-mineralization and cells biocompatibility were investigated using SBF immersion and cells culture methods combined with SEM, XRD and FTIR techniques. The study revealed that this biomimetic scaffold possessed satisfactory functions of cells attachment, bio-mineralization, and cells biocompatibility. The porous structure and the surface of the scaffold which was covered by a bone-like HA crystal layer due to bio-mineralization were profitable for cells attachment and spread.

APA, Harvard, Vancouver, ISO, and other styles

3

Hu, Xueyan, Yuan Man, Wenfang Li, Liying Li, Jie Xu, Roxanne Parungao, Yiwei Wang, et al. "3D Bio-Printing of CS/Gel/HA/Gr Hybrid Osteochondral Scaffolds." Polymers 11, no. 10 (September 30, 2019): 1601. http://dx.doi.org/10.3390/polym11101601.

Full text

Abstract:

Cartilage is an important tissue contributing to the structure and function of support and protection in the human body. There are many challenges for tissue cartilage repair. However, 3D bio-printing of osteochondral scaffolds provides a promising solution. This study involved preparing bio-inks with different proportions of chitosan (Cs), Gelatin (Gel), and Hyaluronic acid (HA). The rheological properties of each bio-ink was used to identify the optimal bio-ink for printing. To improve the mechanical properties of the bio-scaffold, Graphene (GR) with a mass ratio of 0.024, 0.06, and 0.1% was doped in the bio-ink. Bio-scaffolds were prepared using 3D printing technology. The mechanical strength, water absorption rate, porosity, and degradation rate of the bio-scaffolds were compared to select the most suitable scaffold to support the proliferation and differentiation of cells. P3 Bone mesenchymal stem cells (BMSCs) were inoculated onto the bio-scaffolds to study the biocompatibility of the scaffolds. The results of SEM showed that the Cs/Gel/HA scaffolds with a GR content of 0, 0.024, 0.06, and 0.1% had a good three-dimensional porous structure and interpenetrating pores, and a porosity of more than 80%. GR was evenly distributed on the scaffold as observed by energy spectrum analyzer and polarizing microscope. With increasing GR content, the mechanical strength of the scaffold was enhanced, and pore walls became thicker and smoother. BMSCs were inoculated on the different scaffolds. The cells distributed and extended well on Cs/Gel/HA/GR scaffolds. Compared to traditional methods in tissue-engineering, this technique displays important advantages in simulating natural cartilage with the ability to finely control the mechanical and chemical properties of the scaffold to support cell distribution and proliferation for tissue repair.

APA, Harvard, Vancouver, ISO, and other styles

4

Liu, Fwu-Hsing, Ruey-Tsung Lee, Wen-Hsueng Lin, and Yunn-Shiuan Liao. "Selective Laser Sintering of Bio-Metal Scaffold." Procedia CIRP 5 (2013): 83–87. http://dx.doi.org/10.1016/j.procir.2013.01.017.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Kovach, Ildiko, Jens Rumschöttel, Stig E. Friberg, and Joachim Koetz. "Janus emulsion mediated porous scaffold bio-fabrication." Colloids and Surfaces B: Biointerfaces 145 (September 2016): 347–52. http://dx.doi.org/10.1016/j.colsurfb.2016.05.018.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Chen, Xiao Feng, Ying Jun Wang, Chun Rong Yang, and Na Ru Zhao. "Biomimetic Fabrication and Characterization of BG/COL/HCA Scaffolds for Bone Tissue Engineering." Key Engineering Materials 336-338 (April 2007): 1574–76. http://dx.doi.org/10.4028/www.scientific.net/kem.336-338.1574.

Full text

Abstract:

The bone tissue engineering scaffold was developed by compounded the type I collagen with the porous scaffold of the sol-gel derived bioactive glass (BG) in the system CaO-P2O5-SiO2. The resultant porous scaffold was treated in supersaturated calcification solution (SCS) to form the surface layer of hydroxyl-carbonate-apatite (HCA) since the type I collagen possessed good biocompatibility and bio-absorbability, and also, the ability of inducting calcium phosphates to precipitated inside and outside the collagen fibers where the collagen fibers acted as bio-macromolecules template for formation of bone-like inorganic minerals in nature bone such as: octo-calcium phosphate (OCP), tri-calcium phosphate (TCP) and hydroxyl-carbonate-apatite (HCA). On the other hand, the sol-gel derived bioactive glass also played an important role in formation of the above bio-minerals owing to its serial chemical reactions with the body fluid. The in vitro study in supersaturated calcification solution SCS indicated that the surface of the porous scaffold was able to induce formation of bone-like HCA crystals on the pore walls of the scaffold which possessed satisfactory cells biocompatibility.

APA, Harvard, Vancouver, ISO, and other styles

7

Ahsan, AMM, Ruinan Xie, and Bashir Khoda. "Heterogeneous topology design and voxel-based bio-printing." Rapid Prototyping Journal 24, no. 7 (October 8, 2018): 1142–54. http://dx.doi.org/10.1108/rpj-05-2017-0076.

Full text

Abstract:

Purpose The purpose of this paper is to present a topology-based tissue scaffold design methodology to accurately represent the heterogeneous internal architecture of tissues/organs. Design/methodology/approach An image analysis technique is used that digitizes the topology information contained in medical images of tissues/organs. A weighted topology reconstruction algorithm is implemented to represent the heterogeneity with parametric functions. The parametric functions are then used to map the spatial material distribution following voxelization. The generated chronological information yields hierarchical tool-path points which are directly transferred to the three-dimensional (3D) bio-printer through a proposed generic platform called Application Program Interface (API). This seamless data corridor between design (virtual) and fabrication (physical) ensures the manufacturability of personalized heterogeneous porous scaffold structure without any CAD/STL file. Findings The proposed methodology is implemented to verify the effectiveness of the approach and the designed example structures are bio-fabricated with a deposition-based bio-additive manufacturing system. The designed and fabricated heterogeneous structures are evaluated which shows conforming porosity distribution compared to uniform method. Originality/value In bio-fabrication process, the generated bio-models with boundary representation (B-rep) or surface tessellation (mesh) do not capture the internal architectural information. This paper provides a design methodology for scaffold structure mimicking the native tissue/organ architecture and direct fabricating the structure without reconstructing the CAD model. Therefore, designing and direct bio-printing the heterogeneous topology of tissue scaffolds from medical images minimize the disparity between the internal architecture of target tissue and its scaffold.

APA, Harvard, Vancouver, ISO, and other styles

8

Gonzalez, Brittany A., Ariadna Herrera, Claudia Ponce, Marcos Gonzalez Perez, Chia-Pei Denise Hsu, Asad Mirza, Manuel Perez, and Sharan Ramaswamy. "Stem Cell-Secreted Allogeneic Elastin-Rich Matrix with Subsequent Decellularization for the Treatment of Critical Valve Diseases in the Young." Bioengineering 9, no. 10 (October 20, 2022): 587. http://dx.doi.org/10.3390/bioengineering9100587.

Full text

Abstract:

Critical valve diseases in infants have a very poor prognosis for survival. Particularly challenging is for the valve replacement to support somatic growth. From a valve regenerative standpoint, bio-scaffolds have been extensively investigated recently. While bio-scaffold valves facilitate acute valve functionality, their xenogeneic properties eventually induce a hostile immune response. Our goal was to investigate if a bio-scaffold valve could be deposited with tissues derived from allogeneic stem cells, with a specific dynamic culture protocol to enhance the extracellular matrix (ECM) constituents, with subsequent stem cell removal. Porcine small intestinal submucosa (PSIS) tubular-shaped bio-scaffold valves were seeded with human bone marrow-derived mesenchymal stem cells (hBMMSCs), cultured statically for 8 days, and then exposed to oscillatory fluid-induced shear stresses for two weeks. The valves were then safely decellularized to remove the hBMMSCs while retaining their secreted ECM. This de novo ECM was found to include significantly higher (p < 0.05) levels of elastin compared to the ECM produced by the hBMMSCs under standard rotisserie culture. The elastin-rich valves consisted of ~8% elastin compared to the ~10% elastin composition of native heart valves. Allogeneic elastin promotes chemotaxis thereby accelerating regeneration and can support somatic growth by rapidly integrating with the host following implantation. As a proof-of-concept of accelerated regeneration, we found that valve interstitial cells (VICs) secreted significantly more (p < 0.05) collagen on the elastin-rich matrix compared to the raw PSIS bio-scaffold.

APA, Harvard, Vancouver, ISO, and other styles

9

Wang, Siyi, Rong Li, Yongxiang Xu, Dandan Xia, Yuan Zhu, Jungmin Yoon, Ranli Gu, et al. "Fabrication and Application of a 3D-Printed Poly-ε-Caprolactone Cage Scaffold for Bone Tissue Engineering." BioMed Research International 2020 (January 30, 2020): 1–12. http://dx.doi.org/10.1155/2020/2087475.

Full text

Abstract:

Poly-ε-caprolactone (PCL) is a promising synthetic material in bone tissue engineering (BTE). Particularly, the introduction of rapid prototyping (RP) represents the possibility of manufacturing PCL scaffolds with customized appearances and structures. Bio-Oss is a natural bone mineral matrix with significant osteogenic effects; however, it has limitations in being constructed and maintained into specific shapes and sites. In this study, we used RP and fabricated a hollow-structured cage-shaped PCL scaffold loaded with Bio-Oss to form a hybrid scaffold for BTE. Moreover, we adopted NaOH surface treatment to improve PCL hydrophilicity and enhance cell adhesion. The results showed that the NaOH-treated hybrid scaffold could enhance the osteogenesis of human bone marrow-derived mesenchymal stem cells (hBMMSCs) both in vitro and in vivo. Altogether, we reveal a novel hybrid scaffold that not only possesses osteoinductive function to promote bone formation but can also be fabricated into specific forms. This scaffold design may have great application potential in bone tissue engineering.

APA, Harvard, Vancouver, ISO, and other styles

10

Leng, Chong Yan, Yong Shun Cui, Yin Li, Xiao Pei Wu, and Qing Hua Chen. "Investigation of Bio-Mimetic Synthesis SH/KGM/HAP Scaffold." Advanced Materials Research 763 (September 2013): 41–44. http://dx.doi.org/10.4028/www.scientific.net/amr.763.41.

Full text

Abstract:

Sodium hyaluronate / konjac glucomannan (SH/KGM) porous scaffolds were prepared via blending sodium hyaluronate and konjac glucomannan. The ammonia was used as cross-linker in blending process. The SH/KGM scaffolds were soaked into calcium nitrate solution and then followed by immersing into simulated body fluid to get SH/KGM/HAP porous scaffolds. X-ray diffraction and fourier transform infrared spectroscopy (FTIR) were used to characterize the crystallization and chemical structure of SH/KGM and SH/KGM/HAP scaffold materials. The scanning electron microscope (SEM) was used to analysis the morphology of SH/KGM/HAP scaffold and BMSCs on surface of the scaffold. The results show that hydroxyl-apatite produced on the surface of the SH/KGM, which appears as spherical particles in the SH/KGM/HAP scaffold surface, and the SH/KGM/HAP porous scaffold possesses good biocompatibility with cell.

APA, Harvard, Vancouver, ISO, and other styles

11

Golebiowska, Aleksandra A., and Syam P. Nukavarapu. "Bio-inspired zonal-structured matrices for bone-cartilage interface engineering." Biofabrication 14, no. 2 (February 25, 2022): 025016. http://dx.doi.org/10.1088/1758-5090/ac5413.

Full text

Abstract:

Abstract Design and development of scaffold structures for osteochondral (OC) interface regeneration is a significant engineering challenge. Recent efforts are aimed at recapitulating the unique compositional and hierarchical structure of an OC interface. Conventional scaffold fabrication techniques often have limited design control and reproducibility, and the development of OC scaffolds with zonal hierarchy and structural integrity between zones is especially challenging. In this study, a series of multi-zonal and gradient structures were designed and fabricated using three-dimensional bioprinting. We developed OC scaffolds with bi-phasic and tri-phasic configurations to support the zonal structure of OC tissue, and gradient scaffold configurations to enable smooth transitions between the zones to more closely mimic a bone-cartilage interface. A biodegradable polymer, polylactic acid, was used for the fabrication of zonal/gradient scaffolds to provide mechanical strength and support OC function. The formation of the multi-zonal and gradient scaffolds was confirmed through scanning electron microscopy imaging and micro-computed tomography scanning. Precisely controlled hierarchy with tunable porosity along the scaffold length established the formation of the bio-inspired scaffolds with different zones/gradient structure. In addition, we also developed a novel bioprinting method to selectively introduce cells into desired scaffold zones of the zonal/gradient scaffolds via concurrent printing of a cell-laden hydrogel within the porous template. Live/dead staining of the cell-laden hydrogel introduced in the cartilage zone showed uniform cell distribution with high cell viability. Overall, our study developed bio-inspired scaffold structures with structural hierarchy and mechanical integrity for bone-cartilage interface engineering.

APA, Harvard, Vancouver, ISO, and other styles

12

Girgis, Adel S., Padraig D'Arcy, Dalia R. Aboshouk, and Mohamed S. Bekheit. "Synthesis and bio-properties of 4-piperidone containing compounds as curcumin mimics." RSC Advances 12, no. 48 (2022): 31102–23. http://dx.doi.org/10.1039/d2ra05518j.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Wang, Ziyi, and Xin Huang. "Elements of 3D Bioprinting in Periodontal Regeneration: Frontiers and Prospects." Processes 9, no. 10 (September 26, 2021): 1724. http://dx.doi.org/10.3390/pr9101724.

Full text

Abstract:

Periodontitis is a chronic infectious disease worldwide, caused by the accumulation of bacterial plaque, which can lead to the destruction of periodontal supporting tissue and eventually tooth loss. The goal of periodontal treatment is to remove pathogenic factors and control the periodontal inflammation. However, the complete regeneration of periodontal supporting tissue is still a major challenge according to current technology. Tissue engineering recovers the injured tissue through seed cells, bio-capable scaffold and bioactive factors. Three-D-bioprinting is an emerging technology in regeneration medicine/tissue engineering, because of its high accuracy and high efficiency, providing a new strategy for periodontal regeneration. This article represents the materials of 3D bioprinting in periodontal regeneration from three aspects: oral seed cell, bio-scaffold and bio-active factors.

APA, Harvard, Vancouver, ISO, and other styles

14

Shi, Jiajia, Caixia Fan, Yan Zhuang, Jie Sun, Xianglin Hou, Bing Chen, Zhifeng Xiao, et al. "Heparan sulfate proteoglycan promotes fibroblast growth factor-2 function for ischemic heart repair." Biomaterials Science 7, no. 12 (2019): 5438–50. http://dx.doi.org/10.1039/c9bm01336a.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Iwanaga, Shintaroh, Yuta Hamada, Yoshinari Tsukamoto, Kenichi Arai, Taketoshi Kurooka, Shinji Sakai, and Makoto Nakamura. "Design and Fabrication of Mature Engineered Pre-Cardiac Tissue Utilizing 3D Bioprinting Technology and Enzymatically Crosslinking Hydrogel." Materials 15, no. 22 (November 9, 2022): 7928. http://dx.doi.org/10.3390/ma15227928.

Full text

Abstract:

The fabrication of mature engineered cardiac tissue is one of the major challenges in cardiac tissue engineering. For this purpose, we attempted to apply the 3D bioprinting approach. Aiming to construct an oriented tissue, a fine fiber-shaped scaffold with a support structure was first designed using CAD software. Then, a 3D bioprinter and cell-adhesive bio-inks were utilized to fabricate this structure. The cell-adhesive bio-inks were synthesized by combining sodium alginate and gelatin with tyramine, respectively, to form pre-gel materials that allow enzymatic crosslinking by horseradish peroxidase. By absorbance measurements, we confirmed that the tyramine modification rate of each polymer was 0.535 mmol/g-alginate and 0.219 mmol/g-gelatin. The width of the fiber-shaped scaffold was 216.8 ± 24.3 μm for the fabricated scaffold, while the design value was 200 μm. After 3D printing and adhesion-adding treatment of the scaffold with these bio-ink materials, cardiomyocytes were seeded and cultured. As a result, the cells spread onto the scaffold, and the entire pre-tissue contracted synchronously by day 6 of culture, showing a greater pulsatility than in the early days. Video analysis showed that the beating rate of pre-myocardial tissue on day 6 was 31 beats/min. In addition, we confirmed that the cardiomyocytes partially elongated along the long axis of the fiber-shaped scaffold in the pre-tissue cultured for 15 days by staining actin, suggesting the possibility of cell orientation. Furthermore, treatment with adrenaline resulted in a 7.7-fold increase in peak beating rate compared to that before treatment (from 6 beats/min to 46 beats/min), confirming the responsiveness of the pre-tissues to the drug. These results indicate that 3D bioprinting effectively produces mature cultured myocardial tissue that is oriented, contracts synchronously, and is responsive to drugs.

APA, Harvard, Vancouver, ISO, and other styles

16

Mostafavi, Azadeh, Mohamadmahdi Samandari, Mehran Karvar, Mahsa Ghovvati, Yori Endo, Indranil Sinha, Nasim Annabi, and Ali Tamayol. "Colloidal multiscale porous adhesive (bio)inks facilitate scaffold integration." Applied Physics Reviews 8, no. 4 (December 2021): 041415. http://dx.doi.org/10.1063/5.0062823.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Terada, Dohiko, Kazuya Sawada, Kenichi Yoshida, Seiichi Funamoto, Toshiya Fujisato, Akio Kishida, Noritoshi Nagaya, Takeshi Nakatani, and Soichiro Kitamura. "Enzymatic Processing of Structural Proteins for Bio-Scaffold Preparation." Journal of Life Support Engineering 17, Supplement (2005): 98. http://dx.doi.org/10.5136/lifesupport.17.supplement_98.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

YAMASAKI, Kenichi, Dohiko TERADA, Hideo KONDO, Shigehiro HASHIMOTO, and Toshia FUJISATO. "325 Development of bio-actuator using acellular tissue scaffold." Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME 2007.20 (2008): 313–14. http://dx.doi.org/10.1299/jsmebio.2007.20.313.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Parai, Rohan, and Sanchita Bandyopadhyay-Ghosh. "Engineered bio-nanocomposite magnesium scaffold for bone tissue regeneration." Journal of the Mechanical Behavior of Biomedical Materials 96 (August 2019): 45–52. http://dx.doi.org/10.1016/j.jmbbm.2019.04.019.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Nechaev, A., P. Eremin, and I. Gilmutdinova. "BIOACTIVE BIOPLASTIC MATERIAL BASED ON ION-TRACK WOUND COATINGS AND CHITOSAN NANO-SCAFFOLD." http://eng.biomos.ru/conference/articles.htm 1, no. 19 (2021): 22–24. http://dx.doi.org/10.37747/2312-640x-2021-19-22-24.

Full text

Abstract:

The paper presents the results of the development of a bioactive bioplastic material (BIO) based on ion-track wound coatings (ITRP) and a nano-scaffold of chitosan obtained by electroforming. The water and gas permeability, the ultimate strength, bacteriostaticity, cytotoxicity and biocompatibility of BIO ITPR were investigated.

APA, Harvard, Vancouver, ISO, and other styles

21

Lu, Hongyun, Keqin Ying, Ying Shi, Donghong Liu, and Qihe Chen. "Bioprocessing by Decellularized Scaffold Biomaterials in Cultured Meat: A Review." Bioengineering 9, no. 12 (December 9, 2022): 787. http://dx.doi.org/10.3390/bioengineering9120787.

Full text

Abstract:

As novel carrier biomaterials, decellularized scaffolds have promising potential in the development of cellular agriculture and edible cell-cultured meat applications. Decellularized scaffold biomaterials have characteristics of high biocompatibility, bio-degradation, biological safety and various bioactivities, which could potentially compensate for the shortcomings of synthetic bio-scaffold materials. They can provide suitable microstructure and mechanical support for cell adhesion, differentiation and proliferation. To our best knowledge, the preparation and application of plant and animal decellularized scaffolds have not been summarized. Herein, a comprehensive presentation of the principles, preparation methods and application progress of animal-derived and plant-derived decellularized scaffolds has been reported in detail. Additionally, their application in the culture of skeletal muscle, fat and connective tissue, which constitute the main components of edible cultured meat, have also been generally discussed. We also illustrate the potential applications and prospects of decellularized scaffold materials in future foods. This review of cultured meat and decellularized scaffold biomaterials provides new insight and great potential research prospects in food application and cellular agriculture.

APA, Harvard, Vancouver, ISO, and other styles

22

Naseri, Narges, Jean-Michel Poirier, Lenart Girandon, Mirjam Fröhlich, Kristiina Oksman, and Aji P. Mathew. "3-Dimensional porous nanocomposite scaffolds based on cellulose nanofibers for cartilage tissue engineering: tailoring of porosity and mechanical performance." RSC Advances 6, no. 8 (2016): 5999–6007. http://dx.doi.org/10.1039/c5ra27246g.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Krishani, Murugiah, Wong Yen Shin, Hazwani Suhaimi, and Nonni Soraya Sambudi. "Development of Scaffolds from Bio-Based Natural Materials for Tissue Regeneration Applications: A Review." Gels 9, no. 2 (January 23, 2023): 100. http://dx.doi.org/10.3390/gels9020100.

Full text

Abstract:

Tissue damage and organ failure are major problems that many people face worldwide. Most of them benefit from treatment related to modern technology’s tissue regeneration process. Tissue engineering is one of the booming fields widely used to replace damaged tissue. Scaffold is a base material in which cells and growth factors are embedded to construct a substitute tissue. Various materials have been used to develop scaffolds. Bio-based natural materials are biocompatible, safe, and do not release toxic compounds during biodegradation. Therefore, it is highly recommendable to fabricate scaffolds using such materials. To date, there have been no singular materials that fulfill all the features of the scaffold. Hence, combining two or more materials is encouraged to obtain the desired characteristics. To design a reliable scaffold by combining different materials, there is a need to choose a good fabrication technique. In this review article, the bio-based natural materials and fine fabrication techniques that are currently used in developing scaffolds for tissue regeneration applications, along with the number of articles published on each material, are briefly discussed. It is envisaged to gain explicit knowledge of developing scaffolds from bio-based natural materials for tissue regeneration applications.

APA, Harvard, Vancouver, ISO, and other styles

24

Kalirajan, Cheirmadurai, and Thanikaivelan Palanisamy. "A ZnO–curcumin nanocomposite embedded hybrid collagen scaffold for effective scarless skin regeneration in acute burn injury." Journal of Materials Chemistry B 7, no. 38 (2019): 5873–86. http://dx.doi.org/10.1039/c9tb01097a.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Mitsuzawa, Sadaki, Ryosuke Ikeguchi, Tomoki Aoyama, Hisataka Takeuchi, Hirofumi Yurie, Hiroki Oda, Souichi Ohta, et al. "The Efficacy of a Scaffold-free Bio 3D Conduit Developed from Autologous Dermal Fibroblasts on Peripheral Nerve Regeneration in a Canine Ulnar Nerve Injury Model: A Preclinical Proof-of-Concept Study." Cell Transplantation 28, no. 9-10 (June 12, 2019): 1231–41. http://dx.doi.org/10.1177/0963689719855346.

Full text

Abstract:

Autologous nerve grafting is widely accepted as the gold standard treatment for segmental nerve defects. To overcome the inevitable disadvantages of the original method, alternative methods such as the tubulization technique have been developed. Several studies have investigated the characteristics of an ideal nerve conduit in terms of supportive cells, scaffolds, growth factors, and vascularity. Previously, we confirmed that biological scaffold-free conduits fabricated from human dermal fibroblasts promote nerve regeneration in a rat sciatic nerve injury model. The purpose of this study is to evaluate the feasibility of biological scaffold-free conduits composed of autologous dermal fibroblasts using a large-animal model. Six male beagle dogs were used in this study. Eight weeks before surgery, dermal fibroblasts were harvested from their groin skin and grown in culture. Bio 3D conduits were assembled from proliferating dermal fibroblasts using a Bio 3D printer. The ulnar nerve in each dog’s forelimb was exposed under general anesthesia and sharply cut to create a 5 mm interstump gap, which was bridged by the prepared 8 mm Bio 3D conduit. Ten weeks after surgery, nerve regeneration was investigated. Electrophysiological studies detected compound muscle action potentials (CMAPs) of the hypothenar muscles and motor nerve conduction velocity (MNCV) in all animals. Macroscopic observation showed regenerated ulnar nerves. Low-level hypothenar muscle atrophy was confirmed. Immunohistochemical, histological, and morphometric studies confirmed the existence of many myelinated axons through the Bio 3D conduit. No severe adverse event was reported. Hypothenar muscles were re-innervated by regenerated nerve fibers through the Bio 3D conduit. The scaffold-free Bio 3D conduit fabricated from autologous dermal fibroblasts is effective for nerve regeneration in a canine ulnar nerve injury model. This technology was feasible as a treatment for peripheral nerve injury and segmental nerve defects in a preclinical setting.

APA, Harvard, Vancouver, ISO, and other styles

26

Wu, Shuyi, Jieda Wang, Leiyan Zou, Lin Jin, Zhenling Wang, and Yan Li. "A three-dimensional hydroxyapatite/polyacrylonitrile composite scaffold designed for bone tissue engineering." RSC Advances 8, no. 4 (2018): 1730–36. http://dx.doi.org/10.1039/c7ra12449j.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Mahnaz, Fatima, Mohammad Mostafa-Al-Momin, Md Rubel, Md Ferdous, and Md Shafiul Azam. "Mussel-inspired immobilization of Au on bare and graphene-wrapped Ni nanoparticles toward highly efficient and easily recyclable catalysts." RSC Advances 9, no. 52 (2019): 30358–69. http://dx.doi.org/10.1039/c9ra05736f.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Anjarsari, Anjarsari, Kiagus Dahlan, Pipih Suptijah, and Tetty Kemala. "Synthesis and Characterization of Biocomposite BCP/Collagen for Bone Material Scaffold." Jurnal Pengolahan Hasil Perikanan Indonesia 19, no. 3 (December 28, 2016): 356. http://dx.doi.org/10.17844/jphpi.v19i3.14542.

Full text

Abstract:

Biphasic calcium phosphate (BCP) widely used as implants and scaffolds in different orthopedic and dental application. The aim of this study was to determine synthesis and characteristics of biocomposite BCP/collagen as bone scaffold material. BCP/collagen was classified into three groups: 1) BCP/K5 (5% collagen in scaffold), 2) BCP/K10 (10% collagen in scaffold), and 3) BCP/K15 (15% collagen in scaffold). The samples were characterized by Fourier Transform Infrared (FTIR) Spectroscopy, and Scanning Electron Microscope (SEM) techniques. Overall, concentration of collagen was not significantly different to the spectrum. However, FTIR analysis shows the change intensity in bio-composite BCP/collagen. Collagen intensity Higher concentration when collagen concentration in scaffold higher. Morphology analysis of the scaffold showed significant differences in pore formation. BCP/K15 was showed pores formed in scaffold. Synthesis of composite BCP/collagen does not affect the spectrum of functional groups, but affects the formation of pores in the bone scaffold material.

APA, Harvard, Vancouver, ISO, and other styles

29

Anjarsari, Anjarsari, Kiagus Dahlan, Pipih Suptijah, and Tetty Kemala. "Synthesis and Characterization of Biocomposite BCP/Collagen for Bone Material Scaffold." Jurnal Pengolahan Hasil Perikanan Indonesia 19, no. 3 (February 6, 2017): 356. http://dx.doi.org/10.17844/jphpi.v19i3.15113.

Full text

Abstract:

<p>Abstract<br />Biphasic calcium phosphate (BCP) widely used as implants and scaffolds in different orthopedic and dental application. The aim of this study was to determine synthesis and characteristics of biocomposite BCP/collagen as bone scaffold material. BCP/collagen was classified into three groups: 1) BCP/K5 (5% collagen in scaffold), 2) BCP/K10 (10% collagen in scaffold), and 3) BCP/K15 (15% collagen in scaffold). The samples were characterized by Fourier Transform Infrared (FTIR) Spectroscopy, and Scanning Electron Microscope (SEM) techniques. Overall, concentration of collagen was not significantly different to the spectrum. However, FTIR analysis shows the change intensity in bio-composite BCP/collagen. Collagen intensity Higher concentration when collagen concentration in scaffold higher. Morphology analysis of the scaffold showed significant differences in pore formation. BCP/K15 was showed pores formed in scaffold. Synthesis of composite BCP/collagen does not affect the spectrum of functional groups, but affects the formation of pores in the bone scaffold material.</p>

APA, Harvard, Vancouver, ISO, and other styles

30

Arguchinskaya, N. V., E. E. Beketov, E. V. Isaeva, N. S. Sergeeva, P. V. Shegay, S. A. Ivanov, and A. D. Kaprin. "Materials for creating tissue-engineered constructs using 3D bioprinting: cartilaginous and soft tissue restoration." Russian Journal of Transplantology and Artificial Organs 23, no. 1 (April 10, 2021): 60–74. http://dx.doi.org/10.15825/1995-1191-2021-1-60-74.

Full text

Abstract:

3D Bioprinting is a dynamically developing technology for tissue engineering and regenerative medicine. The main advantage of this technique is its ability to reproduce a given scaffold geometry and structure both in terms of the shape of the tissue-engineered construct and the distribution of its components. The key factor in bioprinting is bio ink, a cell-laden biocompatible material that mimics extracellular matrix. To meet all the requirements, the bio ink must include not only the main material, but also other components ensuring cell proliferation, differentiation and scaffold performance as a whole. The purpose of this review is to describe the most common materials applicable in bioprinting, consider their properties, prospects and limitations in cartilage restoration.

APA, Harvard, Vancouver, ISO, and other styles

31

Benayahu, Dafna, Leslie Pomeraniec, Shai Shemesh, Snir Heller, Yoav Rosenthal, Lea Rath-Wolfson, and Yehuda Benayahu. "Biocompatibility of a Marine Collagen-Based Scaffold In Vitro and In Vivo." Marine Drugs 18, no. 8 (August 11, 2020): 420. http://dx.doi.org/10.3390/md18080420.

Full text

Abstract:

Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration. The scaffold aids cell organization in the damaged tissue. It serves and allows bio mimicking the mechanical and biological properties of the target tissue and facilitates cell proliferation and differentiation at the regeneration site. In this study, the developed and assayed bio-composite made of unique collagen fibers and alginate hydrogel supports the function of cells around the implanted material. We used an in vivo rat model to study the scaffold effects when transplanted subcutaneously and as an augment for tendon repair. Animals’ well-being was measured by their weight and daily activity post scaffold transplantation during their recovery. At the end of the experiment, the bio-composite was histologically examined, and the surrounding tissues around the implant were evaluated for inflammation reaction and scarring tissue. In the histology, the formation of granulation tissue and fibroblasts that were part of the inclusion process of the implanted material were noted. At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation. This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair. Overall, the results of the current study proved for the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application.

APA, Harvard, Vancouver, ISO, and other styles

32

Divakar, Prajan, Isabella Caruso, Karen L. Moodie, Regan N. Theiler, P. Jack Hoopes, and Ulrike G. K. Wegst. "Design, Manufacture, and In vivo Testing of a Tissue Scaffold for Permanent Female Sterilization by Tubal Occlusion." MRS Advances 3, no. 30 (2018): 1685–90. http://dx.doi.org/10.1557/adv.2018.57.

Full text

Abstract:

ABSTRACTCurrent FDA-approved permanent female sterilization procedures are invasive and/or require the implantation of non-biodegradable materials. These techniques pose risks and complications, such as device migration, fracture, and tubal perforation. We propose a safe, non-invasive biodegradable tissue scaffold to effectively occlude the Fallopian tubes within 30 days of implantation. Specifically, the Fallopian tubes are mechanically de-epithelialized, and a tissue scaffold is placed into each tube. It is anticipated that this procedure can be performed in less than 30 minutes by an experienced obstetrics and gynaecology practitioner. Advantages of this method include the use of a fully bio-resorbable polymer, low costs, lower risks, and the lack of general anaesthesia. The scaffold devices are freeze-cast allowing for the custom-design of structural, mechanical, and chemical cues through material composition, processing parameters, and functionalization. The performance of the biomaterial and de-epithelialization procedure was tested in an in vivo rat uterine horn model. The scaffold response and tissue-biomaterial interactions were characterized microscopically post-implantation. Overall, the study resulted in the successful fabrication of resilient, easy-to-handle devices with an anisotropic scaffold architecture that encouraged rapid bio-integration through notable angiogenesis, cell infiltration, and native collagen deposition. Successful tubal occlusion was demonstrated at 30 days, revealing the great promise of a sterilization biomaterial.

APA, Harvard, Vancouver, ISO, and other styles

33

Mitić, Vojislav V., Po-Yu Chen, Yueh-Ying Chou, Ivana D. Ilić, Bojana Marković, and Goran Lazović. "Fractal nature analysis in porous structured bio-ceramics." Modern Physics Letters B 35, no. 12 (April 13, 2021): 2150318. http://dx.doi.org/10.1142/s0217984921503188.

Full text

Abstract:

Hydroxyapatite scaffold is a type of bio-ceramic. Its cellular design has similarities with the morphologies in nature. Therefore, it is very important to control the structure, especially the porosity, as one of the main features for bio-ceramics applications. According to some literature, freeze casting can form the shape of dendrites and remain a foam structure after ice sublimation. Ice nucleation became more heterogeneous with the aid of printing materials during freeze casting. This procedure can even improve the issue of crack formation. In this paper, we studied the mechanical properties of hydroxyapatite scaffold. We also analyzed the porosity by fractal nature characterization, and successfully reconstructed pore shape, which is important for predicting ceramic morphology. We applied SEM analysis on bio-ceramic samples, at four different magnifications for the same pore structure. This is important for fractal analysis and pores reconstruction. We calculated the fractal dimensions based on measurements. In this way, we completed the fractal characterization of porosity and confirmed possibilities for successful porous shapes reconstruction. In this paper, we confirmed, for the first time, that fractal nature can be successfully applied in the area of porous bio-ceramics.

APA, Harvard, Vancouver, ISO, and other styles

34

Chakravarty, Sudesna, Nandana Bhardwaj, Biman B. Mandal, and Neelotpal Sen Sarma. "Silk fibroin–carbon nanoparticle composite scaffolds: a cost effective supramolecular ‘turn off’ chemiresistor for nitroaromatic explosive vapours." Journal of Materials Chemistry C 4, no. 38 (2016): 8920–29. http://dx.doi.org/10.1039/c6tc03337g.

Full text

Abstract:

We report the development of a supramolecular bio-nanocomposite material, based on silk fibroin protein scaffold and carbon nanoparticles, as a sustainable sensing platform for nitroaromatic explosive vapours.

APA, Harvard, Vancouver, ISO, and other styles

35

Zhang, Liangwei, Jie Kang, Shudi Liu, Xia Zhang, Jinyu Sun, Yuesong Hu, Yang Yang, and Lingxin Chen. "A chemical covalent tactic for bio-thiol sensing and protein labeling agent design." Chemical Communications 56, no. 77 (2020): 11485–88. http://dx.doi.org/10.1039/d0cc04169f.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Matsumoto, K., R. Machino, D. Taniguchi, Y. Takeoka, Y. Taura, T. Miyazaki, T. Tsuchiya, N. Yamasaki, K. Nakayama, and T. Nagayasu. "Production of Scaffold-free Trachea Tissue by Bio-3D Printer." Nihon Kikan Shokudoka Gakkai Kaiho 67, no. 2 (2016): 106. http://dx.doi.org/10.2468/jbes.67.106.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

Jahnavi, S., T. V. Kumary, G. S. Bhuvaneshwar, T. S. Natarajan, and R. S. Verma. "Engineering of a polymer layered bio-hybrid heart valve scaffold." Materials Science and Engineering: C 51 (June 2015): 263–73. http://dx.doi.org/10.1016/j.msec.2015.03.009.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

Ning, Liqun, and Xiongbiao Chen. "A brief review of extrusion-based tissue scaffold bio-printing." Biotechnology Journal 12, no. 8 (May 24, 2017): 1600671. http://dx.doi.org/10.1002/biot.201600671.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Tsung-Hsuan, Wu,, and Giampietro Bertasi. "The use of Dermacell® in Fingertip Injury." Journal of Clinical Case reports and Images 1, no. 2 (March 14, 2019): 14–22. http://dx.doi.org/10.14302/issn.2641-5518.jcci-19-2626.

Full text

Abstract:

Matrices or tissue scaffolds provide a collagen structure for tissue remodelling while the removal of viable cells aims to minimize or prevent inflammatory or immunogenic response. Allograft collagen scaffold can support the patient’s own cellular ingrowth, ingeneered to minimize an immune response and to yeld a bio-compatible matrix and support incoming cellular growth. The decellyularized dermis retains its growth factors, native collagen scaffold, and elastin, thanks to a LifeNet Health proprietaryprocessin technology.

APA, Harvard, Vancouver, ISO, and other styles

40

WANG, Yulong, Lu HAN, Jia YAN, Kun HU, Luhai LI, Huai ZHANG, and Hao AI. "3D Bioprintability of Konjac Glucomannan Hydrogel." Materials Science 26, no. 1 (November 8, 2019): 109–13. http://dx.doi.org/10.5755/j01.ms.26.1.20336.

Full text

Abstract:

Konjac glucomannan has potential applications in bio-printing, due to its unique properties, such as high viscosity, water-holding capacity and easy gelatinization. In this study, the rheological properties, i.e. the viscosity with changing the shear rate and the storage modulus G’ and loss modulus G’’ curve, of different concentrations of konjac gum hydrogel, were thoroughly measured. Furthermore, the pore sizes of various concentrated konjac gum hydrogel were observed under scanning electron microscopy (SEM). The bio-printability of konjac gum hydrogel was thoroughly evaluated using piston-type 3D bio-printer. It was showed that, 7% konjac gum hydrogel demonstrated the best bio-printability, which has potentially applied as scaffold materials in bio-printing field.

APA, Harvard, Vancouver, ISO, and other styles

41

Qin, Ting Wu, Zhi Ming Yang, Xiu Qun Li, Xiang Tao Mo, Jing Cong Luo, and Hui Qi Xie. "Surface Properties and Cytocompatibility of Bio-derived Compact Bone as Scaffolds for Tissue Engineering Bone." Key Engineering Materials 309-311 (May 2006): 895–98. http://dx.doi.org/10.4028/www.scientific.net/kem.309-311.895.

Full text

Abstract:

Many scaffolds are candidates for use in tissue engineering approaches for the repair or replacement of bone defects. Among the scaffolds tested for tissue engineering of bone, bio-derived compact bone scaffold (BDCBS) containing mineralized collagen fibers, phosphorus and calcium, as natural bone does, is one of the most promising candidates for this purpose. To analyze how appropriate the BDCBS would be for tissue engineering purposes, we established an in vitro characterization system to describe the surface properties and cytocompaibility of the scaffold. Surface properties were determined by means of scanning electron microscope and scanning probe microscope. The surface phase was examined with the Fourier transform infrared spectroscopy and X-ray diffraction. Osteoblasts from human embryos were isolated from the periosteum. After in vitro expansion, cells were cultivated on the BDCBS. Real-time cell culture was used to monitor the growth process of cells seeded on the scaffold. Using this in vitro characterization, we were able to demonstrate effective growth of osteoblasts on this scaffold. In summary, BDCBS has the surface characterization similar to a natural bone and also has strong affinity for osteoblast attachment and proliferation, indicating the potential as an effective scaffold used in tissue engineering bone.

APA, Harvard, Vancouver, ISO, and other styles

42

Wattanutchariya, Wassanai, and Kittiya Thunsiri. "Effects of Fibroin Treatments on Physical and Biological Properties of Chitosan/Hydroxyapatite/Fibroin Bone's Scaffold." Applied Mechanics and Materials 799-800 (October 2015): 488–92. http://dx.doi.org/10.4028/www.scientific.net/amm.799-800.488.

Full text

Abstract:

The development of bio-mimetic scaffold for tissue engineering proposed a novel method to tissue or bone repairing. The biological and physical properties of the scaffold have been recognizing such as biocompatibility, porosity, pore size, and biodegradability. In this work, Chitosan, Hydroxyapatite (HA), and Fibroin were used for bone's scaffold fabrication by freeze drying technique. Those materials are known as biodegradable materials that serve different properties in bone's scaffold. In common fabrication process, the fibroin treatment is requiring for increasing the stiffness of the fibers. Recently, the fibroin treatment is process before the scaffold fabrication. However, the treatment could process after the scaffold fabrication complete. Thus, we compared the biological and physical of the scaffolds between three conditions of fibroin treatment that consist of 1) Non-treatment (NON), 2) Pre-treatment (PRE), and 3) Post-treatment (POST). From the result, both of biological and physical properties, the PRE porous scaffold is the appropriated condition for this research. Finally, we are looking forward to compare the growth of osteoblast cells on the scaffold with different fibroin treatment and aim to implant those scaffolds for bone repairing in the very near future.

APA, Harvard, Vancouver, ISO, and other styles

43

Fu, Na, Zhaosong Meng, Tiejun Jiao, Aihuan Guo, Xiaoding Luo, Ran Wei, Lei Sui, and Xiaoxiao Cai. "Radial P34HB Electrospun Fiber: A Scaffold for Bone Tissue Engineering." Journal of Nanoscience and Nanotechnology 20, no. 10 (October 1, 2020): 6161–67. http://dx.doi.org/10.1166/jnn.2020.18583.

Full text

Abstract:

Finding suitable scaffold material is always an enormous challenge in the study of bone tissue engineering. Designing and preparing bone scaffolds with biomimetic properties is also a difficult problem for bone reparation projects. This project intends to fabricate radial bio-plasticpoly-3-hydroxybutyrate-4-hydroxybutyrate (P34HB) electrospun fibers scaffold that mimic structural, compositional and stiffness properties via an electro spinning technique. The surface morphology, hydrophilicity of the radial fibers scaffold were tested, a contact angle meter and a universal material tester. Bone marrow mesenchymal stem cell (BMSC) morphologies on radial P34HB electrospun fibers scaffold were observed after cell culture under fluorescence microscopy. Tests of cell viability on radial P34HB electrospun fibers scaffold were conducted. We further tested the osteogenic differentiation ability of radial fibers scaffold. These results showed that radial P34HB electrospun fibers scaffold have good biosafety, biocompatibility and osteogenic induction. The radial structure of the scaffold also has a strong effect on the induction of bone formation. Moreover, the structure could also improve the bone contact area of the implant and increase the locking and fixation between the implant and the bone. We plan to apply this procedure to animal experiments for bone defect repair in further research.

APA, Harvard, Vancouver, ISO, and other styles

44

Sjerobabin, Nikola, Bozana Colovic, Milan Petrovic, Dejan Markovic, Slavoljub Zivkovic, and Vukoman Jokanovic. "Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design." Srpski arhiv za celokupno lekarstvo 144, no. 5-6 (2016): 280–87. http://dx.doi.org/10.2298/sarh1606280s.

Full text

Abstract:

Introduction. Biodegradable porous scaffolds are found to be very promising bone substitutes, acting as a temporary physical support to guide new tissue regeneration, until the entire scaffold is totally degraded and replaced by the new tissue. Objective. The aim of this study was to investigate cytotoxicity of a synthesized calcium hydroxyapatite based scaffold, named ALBO-OS, with high porosity and optimal topology. Methods. The ALBO-OS scaffold was synthesized by the method of polymer foam template. The analysis of pore geometry and scaffold walls? topography was made by scanning electron microscope (SEM). The biological investigations assumed the examinations of ALBO-OS cytotoxicity to mouse L929 fibroblasts, using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) and lactate dehydrogenase (LDH) tests and inverse phase microscopy. Results. The SEM analysis showed high porosity with fair pore distribution and interesting morphology from the biological standpoint. The biological investigations showed that the material is not cytotoxic to L929 cells. Comparison of ALBO-OS with Bio-Oss, as the global gold standard as a bone substitute, showed similar results in MTT test, while LDH test showed significantly higher rate of cell multiplication with ALBO-OS. Conclusion. The scaffold design from the aspect of pore size, distribution, and topology seems to be very convenient for cell adhesion and occupation, which makes it a promising material as a bone substitute. The results of biological assays proved that ALBO-OS is not cytotoxic for L929 fibroblasts. In comparison with Bio-Oss, similar or even better results were obtained.

APA, Harvard, Vancouver, ISO, and other styles

45

Fallahiarezoudar, Ehsan, Nor Hasrul Akhmal Ngadiman, Noordin Mohd Yusof, Ani Idris, and Mohamad Shaiful Ashrul Ishak. "Development of 3D Thermoplastic Polyurethane (TPU)/Maghemite (ϒ-Fe2O3) Using Ultra-Hard and Tough (UHT) Bio-Resin for Soft Tissue Engineering." Polymers 14, no. 13 (June 23, 2022): 2561. http://dx.doi.org/10.3390/polym14132561.

Full text

Abstract:

The use of soft tissue engineering scaffolds is an advanced approach to repairing damaged soft tissue. To ensure the success of this technique, proper mechanical and biocompatibility properties must be taken into consideration. In this study, a three-dimensional (3D) scaffold was developed using digital light processing (DLP) and ultra-hard and tough (UHT) bio-resin. The 3D scaffold structure consisted of thermoplastic polyurethane (TPU) and maghemite (ϒ-Fe2O3) nanoparticles mixed with UHT bio-resin. The solution sample for fabricating the scaffolds was varied with the concentration of the TPU (10, 12.5, and 15% wt/v) and the amount of ϒ-Fe2O3 (1, 3, and 5% v/v) added to 15% wt/v of TPU. Before developing the real geometry of the sample, a pre-run of the DLP 3D printing process was done to determine the optimum curing time of the structure to be perfectly cured, which resulted in 30 s of curing time. Then, this study proceeded with a tensile test to determine the mechanical properties of the developed structure in terms of elasticity. It was found that the highest Young’s Modulus of the scaffold was obtained with 15 wt/v% TPU/UHT with 1% ϒ-Fe2O3. Furthermore, for the biocompatibility study, the degradation rate of the scaffold containing TPU/UHT was found to be higher compared to the TPU/UHT containing ϒ-Fe2O3 particles. However, the MTT assay results revealed that the existence of ϒ-Fe2O3 in the scaffold improved the proliferation rate of the cells.

APA, Harvard, Vancouver, ISO, and other styles

46

Gao, Yong Yi, Lan Yun Gong, and Hou An Zhang. "Numerical Simulation of Relationship between Equivalent Elastic Modulus of Porous Titanium Alloy / HA Coating Composite and Fraction Dimension." Applied Mechanics and Materials 34-35 (October 2010): 1988–93. http://dx.doi.org/10.4028/www.scientific.net/amm.34-35.1988.

Full text

Abstract:

The relationships among equivalent elastic modulus of porous titanium alloy / HA coating composite were investigated by using finite element method in this paper. The method of porosity and pore shape of bio-derived bone scaffold materials was ascertained by the elastic modulus of natural bone. The equivalent elastic modulus of porous titanium alloy / HA coating composite decreased with increasing porosity, decreased with the increase of fractal dimension of porosity space, and increased with the increase of fractal dimension of solid matrix. If the porosity ranged from 40% to 50% and pore/solid fractal dimension ranged from 2.77 to 2.96, the equivalent elastic modulus of porous titanium alloy / HA coating composite was consistent with the elastic modulus of natural bone when the bio-derived bone scaffold materials were fabricated by porous titanium alloy / HA composite coating materials.

APA, Harvard, Vancouver, ISO, and other styles

47

Achala Jaglan and YaminiJhanji Dhir. "Tissue Engineering – The Current Scenario & Innovations." International Journal for Modern Trends in Science and Technology 06, no. 9S (October 12, 2020): 54–57. http://dx.doi.org/10.46501/ijmtst0609s08.

Full text

Abstract:

Tissue engineering is an emerging field in medical arena which combines the knowledge of science and engineering to accomplish the increasing demands to aid the damaged tissues or even a whole organ. With time various methods of tissue engineering such as traditional scaffold method ,advanced 3D bioprinting technology and the use of bio ink (the extracellular matrix materials) have become popular at various medical levels. Scaffold is a 3D structure which results in tissue formation by providing space for cells to attach, to proliferate in various directions & by secreting extracellular matrix. Also, the recent development is the use of decellularised extracellular material i.e. dECM as bio-ink to generate vascular organs like Kidney & Heart. Textiles have been playing an indispensable role in tissue engineering as it provide superior methods over other ways to fabricate scaffold. The use of smart biomaterial based scaffolds costs less and is more effective which gives advantage to tailor the tissues according to individual's tissue structure . This paper reviews the application of textiles technology in tissue engineering, various approaches of tissue engineering from traditional to the currently used approach , recent advances and its indications.

APA, Harvard, Vancouver, ISO, and other styles

48

Chimisso, Vittoria, Miguel Angel Aleman Garcia, Saziye Yorulmaz Avsar, Ionel Adrian Dinu, and Cornelia G. Palivan. "Design of Bio-Conjugated Hydrogels for Regenerative Medicine Applications: From Polymer Scaffold to Biomolecule Choice." Molecules 25, no. 18 (September 7, 2020): 4090. http://dx.doi.org/10.3390/molecules25184090.

Full text

Abstract:

Bio-conjugated hydrogels merge the functionality of a synthetic network with the activity of a biomolecule, becoming thus an interesting class of materials for a variety of biomedical applications. This combination allows the fine tuning of their functionality and activity, whilst retaining biocompatibility, responsivity and displaying tunable chemical and mechanical properties. A complex scenario of molecular factors and conditions have to be taken into account to ensure the correct functionality of the bio-hydrogel as a scaffold or a delivery system, including the polymer backbone and biomolecule choice, polymerization conditions, architecture and biocompatibility. In this review, we present these key factors and conditions that have to match together to ensure the correct functionality of the bio-conjugated hydrogel. We then present recent examples of bio-conjugated hydrogel systems paving the way for regenerative medicine applications.

APA, Harvard, Vancouver, ISO, and other styles

49

Yin, Wen, Weiwei Xue, Hecheng Zhu, He Shen, Zhifeng Xiao, Shuyu Wu, Yannan Zhao, et al. "Scar tissue removal-activated endogenous neural stem cells aid Taxol-modified collagen scaffolds in repairing chronic long-distance transected spinal cord injury." Biomaterials Science 9, no. 13 (2021): 4778–92. http://dx.doi.org/10.1039/d1bm00449b.

Full text

Abstract:

Only the first scar tissue removal is a key time point for chronic complete SCI repair. Endogenous NSCs could be intensively activated after the first scar tissue removal and contribute to the chronic SCI repair after bio-scaffold implantation.

APA, Harvard, Vancouver, ISO, and other styles

50

Xu, Zexian, Yali Li, Dian Xu, Li Li, Yaoxiang Xu, Liqiang Chen, Yanshan Liu, and Jian Sun. "Improvement of mechanical and antibacterial properties of porous nHA scaffolds by fluorinated graphene oxide." RSC Advances 12, no. 39 (2022): 25405–14. http://dx.doi.org/10.1039/d2ra03854d.

Full text

Abstract:

Nano-hydroxyapatite (nHA) is widely used as a bio-scaffold material. In this study, fluorinated graphene oxide (FG) was added to nHA to improve its poor formability, weak mechanical properties and undesirable antimicrobial activity that affect its clinical application.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Bio-scaffold'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles