Journal articles on the topic 'Bio-physical constraints'

The study was conducted in Sawombung Block of Imphal East District, Manipur. The present study was undertaken with an objective to analyse the constraints faced by the CAU-R1 rice growers in adoption of the rice variety CAU-R1. The results revealed that the CAU-R1 rice growers faced bio-physical, socio-economic, technological and institutional constraints more severely. In case of bio-physical constraints, inadequate irrigation facilities (98.00%) stood first, followed by weed problems (97.00%),incidence of insect pest and diseases (86.00%) and lack of certified seed (53.00%). In case of socio-economic constraints, high cost of inputs (96.00%) was found to be the first constraint followed by non-availability of credit (93.00%), low market value (86.00%), lack of subsidy for inputs (85.00%) and high labour charge (76.00%). In case of technological constraints, unavailability of power tiller for ploughing in time (96.00%) was found to be the first constraint followed by lack of knowledge of package of practices (82.00%) and lack of technical help/ less contact with technical expert (76.00%). In case of institutional constraints, non-availability of insurance when crop fails (100.00%) was found to be the first constraint followed by insufficient training programme (96.00%), lack of co-ordination with Department of Agriculture, Marketing cooperatives and CAU-R1 growers (63.00%), lack of marketing (58.00%) and lack of storage facilities (35.00%).

Biological organisms do not evolve to perfection, but to out compete others in their ecological niche, and therefore survive and reproduce. This paper reviews the constraints imposed on imperfect organisms, particularly on their neural systems and ability to capture and process information accurately. By understanding biological constraints of the physical properties of neurons, simpler and more efficient artificial neural networks can be made (e.g., spiking networks will transmit less information than graded potential networks, spikes only occur in nature due to limitations of carrying electrical charges over large distances). Furthermore, understanding the behavioural and ecological constraints on animals allows an understanding of the limitations of bio-inspired solutions, but also an understanding of why bio-inspired solutions may fail and how to correct these failures.

The kiwi fruit is a temperate fruit crop; its cultivation is limited to certain temperate regions. The study was conducted during December 2019 to February 2020 in Lower Subansiri District, Arunachal Pradesh to assess the training need areas of kiwi growers wherein a total of 104 farmers were drawn as respondents through random sampling. The response collected from 104 respondents showed that majority (61.54%) of the respondents had exhibited medium level of training needs. Plant protection measures, propagation and planting, and climate and soil were the top three areas perceived as priority areas in regard to training need areas of the respondents, and the least training need area was in fl owering and pollination. The study also revealed that weed problems under bio-physical constraints; high cost of inputs under socio-economic constraints; lack of improved irrigation system and lack of suitable technology for the region under technological constraints; non-availability of insurance under institutional constraints were among the many constraints perceived by the kiwi growers

Various methods of physical, chemical and combined physicochemical pre-treatments for lignocellulosic biomass waste valorisation to value-added feedstock/solid fuels for downstream processes in chemical industries have been reviewed. The relevant literature was scrutinized for lignocellulosic waste applicability in advanced thermochemical treatments for either energy or liquid fuels. By altering the overall naturally occurring bio-polymeric matrix of lignocellulosic biomass waste, individual components such as cellulose, hemicellulose and lignin can be accessed for numerous downstream processes such as pyrolysis, gasification and catalytic upgrading to value-added products such as low carbon energy. Assessing the appropriate lignocellulosic pre-treatment technology is critical to suit the downstream process of both small- and large-scale operations. The cost to operate the process (temperature, pressure or energy constraints), the physical and chemical structure of the feedstock after pre-treatment (decomposition/degradation, removal of inorganic components or organic solubilization) or the ability to scale up the pre-treating process must be considered so that the true value in the use of bio-renewable waste can be revealed.

The recent advancement in information technology and evolving of the (IoT) shifted the traditional medical approach to patient-oriented approach (e.g., Telemedicine/Telemonitoring). IoT permits several services including sensing, processing and communicating information with physical and bio-medical constraints. Wireless Body Area Network (WBAN) handles the issues pertaining to the medical purposes in the form of sensor nodes and connected network. The WBAN takes human physiological data as an input to subsequently monitor the patient conditions that are transferred to other IoT components for analysis. Such monitoring and analysis demand a cohesive routing approach to ensure the safe and in-time transfer of data. The temperature rise of bio-medical sensor nodes makes the entire routing operation very crucial because the temperature of implanted nodes rises and ultimately damages body tissues. This needs dispersion in data transmission among different nodes by opting various available routes while avoiding temperature rise. In this paper, we present Adaptive Thermal-Aware Routing algorithm for WBAN. The ATAR is designed to overcome the temperature rise issue of implanted bio-medical sensors nodes. The new protocol is based on Multi-Ring Routing approach to find an alternative route in the case of increasing temperature. The simulation results indicate that proposed protocol is more efficient in terms of temperature rise and throughput than existing approaches.

Over the past few years, unmanned aerial vehicles (UAV) or drones have been used for many applications. In certain applications like surveillance and emergency rescue operations, multiple drones work as a network to achieve the target in which any one of the drones will act as the master or coordinator to communicate, monitor, and control other drones. Hence, drones are energy-constrained; there is a need for effective coordination among them in terms of decision making and communication between drones and base stations during these critical situations. This paper focuses on providing an efficient approach for the election of the cluster head dynamically, which heads the other drones in the network. The main objective of the paper is to provide an effective solution to elect the cluster head among multi drones at different periods based on the various physical constraints of drones. The elected cluster head acts as the decision-maker and assigns tasks to other drones. In a case where the cluster head fails, then the next eligible drone is re-elected as the leader. Hence, an optimally distributed solution proposed is called Bio-Inspired Optimized Leader Election for Multiple Drones (BOLD), which is based on two AI-based optimization techniques. The simulation results of BOLD compared with the existing Particle Swarm Optimization-Cluster head election (PSO-C) in terms of network lifetime and energy consumption, and from the results, it has been proven that the lifetime of drones with the BOLD algorithm is 15% higher than the drones with PSO-C algorithm.

Profitability of sheep production systems depends on several different animal characteristics rather than a single trait. Economic selection indexes combine information from more than one trait into an overall score, to maximise genetic gain. Economic values (EVs) are required for each trait in the breeding goal so that selection emphasis is proportional to the economic importance of each trait. Defining clear breeding goals is more complex for hill breeds than for other sectors of the sheep industry because they provide breeding females in addition to lambs for slaughter. The aims of this paper are to i) describe how EVs for breeding goal traits suitable for UK hill sheep were derived for a combination of carcass, maternal and ‘sustainability’ traits using a bio-economic model, and ii) show how these EVs vary between different production systems as a result of the differences in the physical constraints of farm size, pasture availability and the biological limits of sheep in extensive rearing environments.

Periodontitis is a serious chronic inflammatory condition that can cause periodontal tissue deterioration and, eventually, tooth loss. Periodontal regenerative therapy using membranes and bone grafting materials, as well as flap debridement and/or flap curettage, have all been used with varying degrees of clinical effectiveness. Current resorbable and non-resorbable membranes serve as a physical barrier, preventing connective and epithelial tissue down growth into the defect and promoting periodontal tissue regeneration. The "perfect" membrane for use in periodontal regenerative therapy has yet to be created, as these conventional membranes have several structural, mechanical, and bio-functional constraints. We hypothesised in this narrative review that the next-generation of guided tissue and guided bone regeneration (GTR/GBR) membranes for periodontal tissue engineering will be a graded-biomaterials that closely mimics the extracellular matrix.

<p>Lignocellulosic biomass (LCB) such as crop residues and agro-industrial wastes are renewable resources and available abundantly. It could play central role in sustainable feeding system of ruminant production. These materials are potential source of fiber to support optimum rumen function and energy supply. However, the LCB has important nutritional constraints that limit its utilization as feed for ruminants. This review is intended to discuss nutritional constraints of LCB as ruminant feed and the potentials and challenges of processes in upgrading the nutritional quality of LCB. The main polymer compounds in LCB are cellulose (30 to 60% ), hemicellulose (20 to 40 %) and lignin (15-25%) and its inter linkages make the energy contained in the LCB is less extractable by the ruminant digestive system. Physical, chemical and biological processing technologies have been well known as alternative means to upgrade the nutritive values of LCB. Recently, novel processing technologies of LCB such as ionic liquid, organosolv, sonication, and new screened rot white fungi (<em>Ceriporiopsis subvermispora</em>) and some older technologies using alkaline and acids have been studied and developed particularly for the purpose of biofuel production in the bio refinery industry. Processing technologies have different properties in degrading the lignin, degrading and solubilizing the cellulose and hemicellulose that all relate to the nutritive quality of LCB. Advantages of processed LCB in ruminant animals were indicated by increase in diet digestibility, intake, rumen fermentation and gain. It is concluded that numerous processing technologies are available to upgrade the nutritional quality of LCB, but there are obstacles to use some of these techniques for wide application in ruminant production system.</p>

Historic urban landscapes are bio-physical imprints of past generation’s activities as well as a matrix for those of current and future generations. Pressures of economic development, while benefitting modernization, have led to loss of traditional practices in cultural landscapes, which reflected customs, values and belief systems of communities. This article explores alternative ways of seeing historic cities particularly with respect to the historic urban landscape paradigm. Chamba town, Himachal Pradesh, a pilgrim centre, market town and historic capital of the Chamba region, recently celebrated the millennium of its establishment. This historic town has distinctive location and is characterized by exceptional cultural and natural resources, landforms, bio-diversity and intangible cultural heritage. With a current population of 25,000, it is growing as a major town centre and pushing the boundaries of town limits and residential areas. Unregulated new development which is not designed for earthquake resistance, absence of delineation of ecologically sensitive zones, marginalization of traditional knowledge and aspirations of the primary custodians have resulted in fragile state of being, both naturally and culturally, and have altered the traditional regime of this historic town. This article examines the fragile relation between humans, nature and culture of Chamba and need for sustainable planning and management system for heritage resources within mountainous regions, with responsiveness to constraints and challenges. The authors reflect upon economic shifts, cultural transformations and infrastructure development, which adapt traditional knowledge designs to address urban needs for historic urban landscapes.

The inertialess fluid–structure interactions of active and passive inextensible filaments and slender-rods are ubiquitous in nature, from the dynamics of semi-flexible polymers and cytoskeletal filaments to cellular mechanics and flagella. The coupling between the geometry of deformation and the physical interaction governing the dynamics of bio-filaments is complex. Governing equations negotiate elastohydrodynamical interactions with non-holonomic constraints arising from the filament inextensibility. Such elastohydrodynamic systems are structurally convoluted, prone to numerical errors, thus requiring penalization methods and high-order spatio-temporal propagators. The asymptotic coarse-graining formulation presented here exploits the momentum balance in the asymptotic limit of small rod-like elements which are integrated semi-analytically. This greatly simplifies the elastohydrodynamic interactions and overcomes previous numerical instability. The resulting matricial system is straightforward and intuitive to implement, and allows for a fast and efficient computation, more than a hundred times faster than previous schemes. Only basic knowledge of systems of linear equations is required, and implementation achieved with any solver of choice. Generalizations for complex interaction of multiple rods, Brownian polymer dynamics, active filaments and non-local hydrodynamics are also straightforward. We demonstrate these in four examples commonly found in biological systems, including the dynamics of filaments and flagella. Three of these systems are novel in the literature. We additionally provide a Matlab code that can be used as a basis for further generalizations.

SUMMARYRice (Oryza sativa L.) is an important staple crop in South Sudan. Current rice consumption in the country is approximately 23,000 mg year−1 of which more than 75% is imported. We investigated farmers’ perceived rice production and productivity constraints and choice of rice ideotypes across rainfed and irrigated rice agro-ecologies in South Sudan to guide breeding and policy interventions. Data were gathered through participatory rural approaches and a formal structured survey involving 136 rice farmers from major rice growing areas of South Sudan. Farmers generally cultivated old rice varieties with low yields varying from 0.4 to 1.6 mg ha−1. Pair-wise ranking based on respondents score indicated yield, early maturity, cooking and eating quality, nutritional importance and drought tolerance as the most desirable traits of rice ideotypes. Further, imported rice varieties were best ranked for their sweet and appealing taste, rich aroma, grain shape and size, swelling capacity and non-stickiness during cooking. Major perceived constraints to rice production were unreliable rainfall, poor access to credit facilities, poor soil and water management practices, poor rice storage facilities, inadequate and poor processing machines as well as limited technical skills in rice production. The results show a need for integration of farmers’ and stakeholders’ preferences in variety development process and the desirability of releasing site-specific rice cultivars given the differences in bio-physical, socio-cultural and farmers preferences across major rice growing areas of South Sudan.

Monocropping, the widely practiced traditional coconut cultivation system in Sri Lanka, utilizes bio-physical resources sub optimally, generating low returns to growers than its potential under an intensive cultivation alternative, coconut-based intercropping (CBI). Despite concerted efforts of successive governments, the adoption of CBI systems by farmers is low. This study investigates the farmers' perceptions of CBI on the premise that the understanding of farmers’ perceptions of a new technology is important to identify the causes for the low adoption of that technology. Data were gathered by a field survey of 113 and 37 intercroppers and monocroppers respectively, in three main coconut-growing districts, namely Kurunegala, Gampaha and Puttalam, using a structured questionnaire supplemented with open-ended questions, through a single visit. Percentage analysis supplemented with a simple scoring device was employed to analyze the farmer's perceptions of CBI. Intercroppers objectives of intercropping, the constraints they face in expanding intercropping, reasons for non-adoption of CBI by present monocroppers and the suggestions of present intercroppers to further expand the CBI, all in the order of importance, are presented.

New strategies in regenerative medicine include the implantation of stem cells cultured in bio-resorbable polymeric scaffolds to restore the tissue function and be absorbed by the body after wound healing. This requires the development of appropriate micro-technologies for manufacturing of functional scaffolds with controlled surface properties to induce a specific cell behavior. The present report focuses on the effect of substrate topography on the behavior of human mesenchymal stem cells (MSCs) before and after co-differentiation into adipocytes and osteoblasts. Picosecond laser micromachining technology (PLM) was applied on poly (L-lactide) (PLLA), to generate different microstructures (microgrooves and microcavities) for investigating cell shape, orientation, and MSCs co-differentiation. Under certain surface topographical conditions, MSCs modify their shape to anchor at specific groove locations. Upon MSCs differentiation, adipocytes respond to changes in substrate height and depth by adapting the intracellular distribution of their lipid vacuoles to the imposed physical constraints. In addition, topography alone seems to produce a modest, but significant, increase of stem cell differentiation to osteoblasts. These findings show that PLM can be applied as a high-efficient technology to directly and precisely manufacture 3D microstructures that guide cell shape, control adipocyte morphology, and induce osteogenesis without the need of specific biochemical functionalization.

The possibility of prolonging space missions—and consequently the permanence of humans in space—depends on the possibility of providing them with an adequate supply of fresh foods to meet their nutritional requirements. This would allow space travelers to mitigate health risks associated with exposure to space radiation, microgravity and psychological stress. In this review, we attempt to critically summarize existing studies with the aim of suggesting possible solutions to overcome the challenges to develop a bio-regenerative life support system (BLSS) that can contribute to life support, supplying food and O2, while removing CO2 on the International Space Station (ISS). We describe the physical constraints and energy requirements for ISS farming in relation to space and energy resources, the problems related to lighting systems and criteria for selecting plants suitable for farming in space and microgravity. Clearly, the dimensions of a growth hardware that can be placed on ISS do not allow to produce enough fresh food to supplement the stored, packaged diet of astronauts; however, experimentation on ISS is pivotal for implementing plant growth systems and paves the way for the next long-duration space missions, including those in cis-lunar space and to the lunar surface.

Bioeconomy strategies in high income societies focus at replacing finite, fossil resources by renewable, biological resources to reconcile macro-economic concerns with climate constraints. However, the current bioeconomy is associated with critical levels of environmental degradation. As a potential increase in biological resource use may further threaten the capacity of ecosystems to fulfil human needs, it remains unclear whether bioeconomy transitions in high income countries are sustainable. In order to fill a gap in bioeconomy sustainability assessments, we apply an ontological lens of coupled social-ecological systems to explore critical mechanisms in relation to bioeconomy activities in the global resource system. This contributes to a social-ecological systems (SES)-based understanding of sustainability from a high income country perspective: the capacity of humans to satisfy their needs with strategies that reduce current levels of pressures and impacts on ecosystems. Building on this notion of agency, we develop a framework prototype that captures the systemic relation between individual human needs and collective social outcomes on the one hand (micro-level) and social-ecological impacts in the global resource system on the other hand (macro-level). The BIO-SES framework emphasizes the role of responsible consumption (for physical health), responsible production (to reduce stressors on the environment), and the role of autonomy and self-organisation (to protect the reproduction capacity of social-ecological systems). In particular, the BIO-SES framework can support (1) individual and collective agency in high income country contexts to reduce global resource use and related ecosystem impacts with a bioeconomy strategy, (2) aligning social outcomes, monitoring efforts and governance structures with place-based efforts to achieve the SDGs, as well as (3), advancing the evidence base and social-ecological theory on responsible bioeconomy transitions in the limited biosphere.

Abstract: Opisthobranchs are marine molluscs apparently unprotected by physical constraints of a shell which is either reduced or completely absent in the adults. Their survival is based on a series of defensive strategies, which involve cryptic behaviour and use of deterrents. During the last twenty years, many studies have been performed to characterize the chemicals isolable from these animals. In this paper we summarize the studies covering two large groups of opistobranch molluscs: sacoglossans and dorids. The main aim is to give to the readers a brief view of the compounds isolated from these molluscs, and, when it is possible, to suggest an ecological role for them. The Order Sacoglossa has been selected as it contains a wide range of morphological types from primitive species with a relatively strong external shell to shell-less types. The chemical studies of these molluscs have been reviewed with the aim of constructing a general scenario based on chemical evidence. Similar reasons prompted us to review the chemical data of the shell-less dorid nudibranchs belonging to the superfamilies Eudoridoidea and Bathydoridoidea. In this case, the review dates from the literature subsequent to Karuso's 1987 review "Chemical Ecology of Nudibranchs". The selection of the two groups, sacoglossans and dorids, is also due to their different, but exclusive feeding habits, green algae for sacoglossans and sponges for dorids. The critical analysis of all these studies illuminates the extraordinary capability of opisthobranch molluscs to create new chemistry through either bio­ accumulation of selected metabolites from their dietary sources, bio-transformation of dietary compounds, or de novo bio-synthesis of useful chemicals. However, it is difficult to determine the boundaries of these investigations. In fact, their ecological contributions are relevant and applications useful for man are foreseable.

Inland valleys are defined as the upper reaches of river systems. These include valley bottoms and minor floodplains,their hydromorphic fringes and upland slopes and crests. These occupy 22-52 million ha of land in W. Africa and although of good agricultural potential are only marginally used. An agro-ecological characterization developed in the Cte d'Ivoire, is outlined on 4 levels: macro (1:1 000 000-1:5 000 000), reconnaissance (1:100 000-1:250 000), semi-detailed (1:25 000-1:50 000) and detailed (1:5000-10 000). Methods of characterization at each of these levels are discussed and examples of actual application given plus the mechanisms applied in disaggregation (scaling down) and aggregation (scaling up) between levels. This approach allows: (1) a systematic description of different inland valley agro-ecosystems; (2) identification of constraints to agricultural use; (3) targeting and implemention of research; and (4) extrapolation of research results and transfer of new technology to similar areas. Studies carried out show variation in (bio)physical and land use factors is considerable. The set of descriptors now developed allows for: (1) extrapolation of the relation between biophysical driving factors and actual land use; (2) identification of geographical areas where improved management is promising; and (3) indications on the type of improvements required.

Maximizing the production of ruminants requires high intakes of feeds that are energy dense, therefore feeds that are usually high in starch. Under these conditions there can be significant passage of partially digested feed starch and fibre to the small intestine. These digesta components interact in a manner that can diminish post-ruminal digestion and absorption. However, in ruminants, the effects of digesta composition on causing physical constraints to digestion and absorption, via increased digesta viscosity have not been given adequate consideration. While elevation of digesta viscosity as a means of reducing rate of nutrient absorption and re-absorption of compounds detrimental to health is a valid approach in humans, digesta viscosity may reduce the production potential of livestock. Thus, there is merit in reviewing the feeding strategies that lead to increased viscosity and how its mitigation can be managed through inclusion of oil in ruminant diets. The interactions between digesta composition and the resultant viscosity with digestive physiology and production factors are examined. This review also highlights the associated benefits of dietary oil for ruminants, such as enhancement of bio-active lipids in ruminant products, health benefits directly to the animal, through decreasing incidence of liver abscesses and effects on production and possibly on the environment (reduction of enteropathogenic bacterial shedding and methane emissions). Key words: Ruminant, starch, digesta characteristics, viscosity, cholecystokinin

In this article, we summarize and analyze the constraints and technical difficulties of biogas plants in clusters, storage and transportation yards, pretreatment yards, and anaerobic fields. Combined with Tyre’s own experience, several major techniques have been proposed. To guide and recommend the suitable technicians to decision makers, to acquire operation courses suitable for the general requirements of our biogas, and to promote the development of booth biogas seedlings in China, the use of environmental slam objects in a small and sufficient nonscale biogas program is formulated by the use of biogas funding alone. Current rating measures leverage the World Age Assessment (LCA) for the Integrated Booth Biogas Swing Type (typically 40,000 kWh) and Bio-Natural Gasoline Program (daily gasoline workload of 12,000 cubic curves). This is built upon the Environmental Impact Assessment and Analogy for Warships Both environmental stroke and sensitivity component analysis. These techniques include termination outlines, register analysis, dynamism drops for each performance stagecoach, and environmental issue calculations. According to the above summarization, when biogas dominates the family, the highest environmental emission becomes the biogas fermentation layer of the booth. Meanwhile, the most sympathetic substitute is the work cost of physical and chemical farting (mL g-1TS). The above two indicators denote the essence of biogas purification, and the choice of biogas purification technology. By evaluating the change in the impact of suppressing environmental emissions, the biogas substitute of scorpion charcoal has a remarkable improvement in the biogas equivalent of the charcoal-bright swing family. Comprehensive experimental results have demonstrated this conclusion.

The problem of shortage of water to crops can be resolved by increasing total water supply available to plants, increasing water use relative to other losses and efficient management of scarce water. Biophysically, solutions to many of the problems will require the improvement of soil, water, and crop management at the field, plot, and farm level: first, to increase the capture and retention of incoming (rain) water; and second, to maximize the proportion of that water productively transpired by the crop. Dry land agriculture under rain fed conditions is found mainly in Africa, the Middle East, Asia, and Latin America. In the harsh environments of Sub-Saharan Africa (SSA) and West Asia and North Africa (WANA), water is the principal factor limiting crop yield. A review has been carried out on soil and crop management research that can increase the water use efficiency. The WANA production systems are dominated by cereals, primarily wheat in the wetter and barley in the drier areas, in rotation with mainly food legumes such as chickpea, lentil and forage legumes. The SSA production systems are generally characterized by cereal/legume mixed-cropping dominated by maize, millet, sorghum, and wheat. The major constraints in both regions to crop production are low soil fertility, insecure rainfall, and low-productive genotypes, low adoption of improved soil and crop management practices, and lack of appropriate institutional support. Different cropping systems and accompanying technologies are discussed. Results indicate that there is an advantage to apply these technologies but being function of socio-economic and bio-physical conditions. It is recommended that future research focuses on integrated technology development while taking into account also different levels of scale such as field, village, and watershed.

IntroductionIn the United Kingdom(1) and internationally(2), help-seeking for domestic violence (DV) and domestic homicides have increased(3) during COVID-19 lockdown periods. Suspension and remote delivery of face-to-face clinical services, continuing healthcare and other support services limits opportunities for DV detection and disclosure.MethodsThis presentation will summarise changes in DV incidence and help-seeking during COVID-19, their impacts on health and wellbeing, and present guidance for clinicians assessing and supporting survivors.ResultsWorld Health Organisation recommendations to Listen, Inquire, Validate, Enhance safety and Support (’LIVES’) survivors of DV remain the cornerstone of first-line support (4). Urgently-issued guidelines on safeguarding(5) and responding to DV during COVID-19(6) make a range of recommendations for clinicians supporting people experiencing DV.ConclusionsDV is an important social determinant of physical and mental health, with a range of potential fatal and non-fatal consequences. Despite the constraints of healthcare during a pandemic, attention to patients’ risk of DV and its consequences is a crucial part of bio-psycho-social assessment and management planning. References: (1) Kelly, Morgan. Coronavirus: Domestic abuse calls up 25% since lockdown, charity says. 2020. https://www.bbc.co.uk/news/uk-52157620 (2) Graham-Harrison, et al. Lockdowns around the world bring rise in domestic violence. 2020. https://www.theguardian.com/society/2020/mar/28/lockdowns-world-rise-domestic-violence (3) Roesch, et al. Violence against women during covid-19 pandemic restrictions. BMJ 2020;369. (4) WHO. Responding to intimate partner violence and sexual violence against women: WHO clinical and policy guidelines. 2013. https://apps.who.int/iris/bitstream/handle/10665/85240/9789241548595_eng.pdf;jsessionid=E19DCC3CDAB9BE390EE6F8360C6F1D7E?sequence=1 (5) RCGP. COVID-19 and Safeguarding. 2020. https://elearning.rcgp.org.uk/pluginfile.php/149180/mod_resource/content/2/COVID-19%20and%20Safeguarding%20%286%29.pdf (6) IRISi. Guidance for General Practice teams responding to domestic abuse during telephone and video consultations. 2020. https://irisi.org/wp-content/uploads/2020/04/Guidance-for-General-Practice-Covid-19-FINAL.pdfDisclosureNo significant relationships.

Internet of Things (IoT) and Cyber-Physical Systems (CPS) have profoundly influenced the way individuals and enterprises interact with the world. Although attacks on IoT devices are becoming more commonplace, security metrics often focus on software, network, and cloud security. For CPS systems employed in IoT applications, the implementation of hardware security is crucial. The identity of electronic circuits measured in terms of device parameters serves as a fingerprint. Estimating the parameters of this fingerprint assists the identification and prevention of Trojan attacks in a CPS. We demonstrate a bio-inspired approach for hardware Trojan detection using unsupervised learning methods. The bio-inspired principles of pattern identification use a Spiking Neural Network (SNN), and glial cells form the basis of this work. When hardware device parameters are in an acceptable range, the design produces a stable firing pattern. When unbalanced, the firing rate reduces to zero, indicating the presence of a Trojan. This network is tunable to accommodate natural variations in device parameters and to avoid false triggering of Trojan alerts. The tolerance is tuned using bio-inspired principles for various security requirements, such as forming high-alert systems for safety-critical missions. The Trojan detection circuit is resilient to a range of faults and attacks, both intentional and unintentional. Also, we devise a design-for-trust architecture by developing a bio-inspired device-locking mechanism. The proposed architecture is implemented on a Xilinx Artix-7 Field Programmable Gate Array (FPGA) device. Results demonstrate the suitability of the proposal for resource-constrained environments with minimal hardware and power dissipation profiles. The design is tested with a wide range of device parameters to demonstrate the effectiveness of Trojan detection. This work serves as a new approach to enable secure CPSs and to employ bio-inspired unsupervised machine intelligence.

Understanding the pattern of species distribution and the underlying mechanism is essential for conservation planning. Several climatic variables determine the species diversity, and the dependency of species on climate motivates ecologists and bio-geographers to explain the richness patterns along with elevation and environmental correlates. We used interpolated elevational distribution data to examine the relative importance of climatic variables in determining the species richness pattern of 26 species of gymnosperms in the longest elevation gradients in the world. Thirteen environmental variables were divided into three predictors set representing each hypothesis model (energy-water, physical-tolerance, and climatic-seasonality); to explain the species richness pattern of gymnosperms along the elevational gradient. We performed generalized linear models and variation partitioning to evaluate the relevant role of environmental variables on species richness patterns. Our findings showed that the gymnosperms’ richness formed a hump-shaped distribution pattern. The individual effect of energy-water predictor set was identified as the primary determinant of species richness. While, the joint effects of energy-water and physical-tolerance predictors have explained highest variations in gymnosperm distribution. The multiple environmental indicators are essential drivers of species distribution and have direct implications in understanding the effect of climate change on the species richness pattern.

The development of free-range chicken farm is constrained by expensive food prices. On the other hand,feses of cattle had potential use as a source of feed. This study was conducted to determine the response ofcow feses and probiotics administration in the diet of laying range chicken. The study was conducted over 6months using 240 free-range chickens aged 8 months. Research was arranged in a completely randomized designwith 8 treatments and 5 replications. The treatment given were ration in accordance with the farmers formulacomprising: concentrate: 25%, corn 40% and rice bran 35% (P0) and ration as P0, with the substitution of ricebran with fermented cow feses each treatment with level 10 % (P1), 15% (P2) and 20% (P3) and ration as atP0, P1, P2 and P3 that received additional probiotic (Bio L) 1 cc / L of drinking water (P4, P5, P6 and P7). Theparameters were observed are: egg production (hen day), egg weight, feed intake, FCR (Feed Conversion Ratio)mortality and physical composition of eggs. The results showed that the diet which containing fermented cowfeses 20% did not decreased egg production and feed efficiency. The combination of fermented cow feses at thelevel of 20% with a probiotic (Bio L) 1 cc / L of drinking water showed that egg production increased althoughit is not significant and reduce the number of FCR (Feed Conversion Ratio). In conclusion the use of fermentedcow feses up to the level of 20% in the diet both with and without the administration of probiotics, has no effecton physical composition of eggs.

To reduce human risk and maintenance costs, Autonomous Underwater Vehicles (AUVs) are involved in subsea inspections and measurements for a wide range of marine industries such as offshore wind farms and other underwater infrastructure. Most of these inspections may require levels of manoeuvrability similar to what can be achieved by tethered vehicles, called Remotely Operated Vehicles (ROVs). To extend AUV intervention time and perform closer inspection in constrained spaces, AUVs need to be more efficient and flexible by being able to undulate around physical constraints. A biomimetic fish-like AUV known as RoboFish has been designed to mimic propulsion techniques observed in nature to provide high thrust efficiency and agility to navigate its way autonomously around complex underwater structures. Building upon advances in acoustic communications, computer vision, electronics and autonomy technologies, RoboFish aims to provide a solution to such critical inspections. This paper introduces the first RoboFish prototype that comprises cost-effective 3D printed modules joined together with innovative magnetic coupling joints and a modular software framework. Initial testing shows that the preliminary working prototype is functional in terms of water-tightness, propulsion, body control and communication using acoustics, with visual localisation and mapping capability.

Abstract. The North Atlantic spring bloom is one of the main events that lead to carbon export to the deep ocean and drive oceanic uptake of CO2 from the atmosphere. Here we use a suite of physical, bio-optical and chemical measurements made during the 2008 spring bloom to optimize and compare three different models of biological carbon export. The observations are from a Lagrangian float that operated south of Iceland from early April to late June, and were calibrated with ship-based measurements. The simplest model is representative of typical NPZD models used for the North Atlantic, while the most complex model explicitly includes diatoms and the formation of fast sinking diatom aggregates and cysts under silicate limitation. We carried out a variational optimization and error analysis for the biological parameters of all three models, and compared their ability to replicate the observations. The observations were sufficient to constrain most phytoplankton-related model parameters to accuracies of better than 15%. However, the lack of zooplankton observations leads to large uncertainties in model parameters for grazing. The simulated vertical carbon flux at 100 m depth is similar between models and agrees well with available observations, but at 600 m the simulated flux is much larger for the model with diatom aggregation. While none of the models can be formally rejected based on their misfit with the available observations, the model that includes export by diatom aggregation has slightly better fit to the observations and more accurately represents the mechanisms and timing of carbon export based on observations not included in the optimization. Thus models that accurately simulate the upper 100 m do not necessarily accurately simulate export to deeper depths.

Abstract. The North Atlantic spring bloom is one of the main events that lead to carbon export to the deep ocean and drive oceanic uptake of CO2 from the atmosphere. Here we use a suite of physical, bio-optical and chemical measurements made during the 2008 spring bloom to optimize and compare three different models of biological carbon export. The observations are from a Lagrangian float that operated south of Iceland from early April to late June, and were calibrated with ship-based measurements. The simplest model is representative of typical NPZD models used for the North Atlantic, while the most complex model explicitly includes diatoms and the formation of fast sinking diatom aggregates and cysts under silicate limitation. We carried out a variational optimization and error analysis for the biological parameters of all three models, and compared their ability to replicate the observations. The observations were sufficient to constrain most phytoplankton-related model parameters to accuracies of better than 15 %. However, the lack of zooplankton observations leads to large uncertainties in model parameters for grazing. The simulated vertical carbon flux at 100 m depth is similar between models and agrees well with available observations, but at 600 m the simulated flux is larger by a factor of 2.5 to 4.5 for the model with diatom aggregation. While none of the models can be formally rejected based on their misfit with the available observations, the model that includes export by diatom aggregation has a statistically significant better fit to the observations and more accurately represents the mechanisms and timing of carbon export based on observations not included in the optimization. Thus models that accurately simulate the upper 100 m do not necessarily accurately simulate export to deeper depths.

AbstractFor the agricultural industry in Slovakia, the dominating sector is crop production. The major part of arable land is devoted to the cultivation of cereals (57%), feed crops (20%) and industry crops (19%).The aim of this paper is to model the distribution of crop management practices in selected regions of Slovakia and identify the net return for four selected crops in these regions. We use integrated modelling framework which incorporates bio-physical and economic data to identify opportunity costs of agricultural production choices. We delineate homogenous response units (HRU) and aggregate them on regional level as the model is constrained by land endowment in particular region. The results suggest that highest yields and thus high returns are achieved in case of management practice with high nitrogen input and irrigation. The high input management causes environmental pressures on soils, therefore its enforcement in regions is not desirable. The solution might appear in policy premiums for low input management practices.

In-situ rehabilitation of fly ash at dumping sites has rarely been addressed for crop production due to growth-related constraints, largely of heavy metal (HM) contamination in soils and crops. Current communication deals with a novel approach to identify a suitable management option for rejuvenating the contaminated soils. In this background, a 60-days incubation experiment was conducted with different fly ash-soil mixtures (50 + 50%, A1; 75 + 25%, A2; 100 + 0%, A3) along with four ameliorants, namely, lime (T1), sodium sulphide (T2), di-ammonium phosphate (T3), and humic acid (T4) at 30 ± 2 °C to assess the ability of different fly ash-soil-ameliorant mixtures in reducing bio-availability of HMs. Diethylenetriaminepentaacetic acid (DTPA)-extractable bio-available HM contents for lead (Pb), cadmium (Cd), nickel (Ni), and chromium (Cr) and their respective ratios to total HM contents under the influence of different treatments were estimated at 0, 15, 30, 45, and 60 days of incubation. Further, the eco-toxicological impact of different treatments on soil microbial properties was studied after 60 days of experimentation. A1T1 significantly recorded the lowest bio-availability of HMs (~49–233% lower) followed by A2T1 (~35–133%) among the treatments. The principal component analysis also confirmed the superiority of A1T1 and A2T1 in this regard. Further, A1T1 achieved low contamination factor and ecological risk with substantial microbial biomass carbon load and dehydrogenase activity. Thus, liming to fly ash-soil mixture at 50:50 may be considered as the best management option for ameliorating metal toxicity. This technology may guide thermal power plants to provide the necessary package of practices for the stakeholders to revive their contaminated lands for better environmental sustainability.

Active vibrissal touch can be used to replace or to supplement sensory systems such as computer vision and, therefore, improve the sensory capacity of mobile robots. This paper describes how arrays of whisker-like touch sensors have been incorporated onto mobile robot platforms taking inspiration from biology for their morphology and control. There were two motivations for this work: first, to build a physical platform on which to model, and therefore test, recent neuroethological hypotheses about vibrissal touch; second, to exploit the control strategies and morphology observed in the biological analogue to maximize the quality and quantity of tactile sensory information derived from the artificial whisker array. We describe the design of a new whiskered robot, Shrewbot , endowed with a biomimetic array of individually controlled whiskers and a neuroethologically inspired whisking pattern generation mechanism. We then present results showing how the morphology of the whisker array shapes the sensory surface surrounding the robot's head, and demonstrate the impact of active touch control on the sensory information that can be acquired by the robot. We show that adopting bio-inspired, low latency motor control of the rhythmic motion of the whiskers in response to contact-induced stimuli usefully constrains the sensory range, while also maximizing the number of whisker contacts. The robot experiments also demonstrate that the sensory consequences of active touch control can be usefully investigated in biomimetic robots.

Abstract The objective of this paper is to present an automatic monitoring system for the 3D CEMBS model in the operational version. This predictive, eco hydrodynamic model is used as a tool to control the conditions and bio productivity of the Baltic sea environment and to forecast physical and ecological changes in the studied basin. Satellite-measured data assimilation is used to constrain the model and achieve higher accuracy of its results. 3D CEMBS is a version of the Community Earth System Model, adapted for the Baltic Sea. It consists of coupled ocean and ice models, working in active mode together with the ecosystem module. Atmospheric forecast from the UM model (Interdisciplinary Centre for Mathematical and Computational Modelling of the Warsaw University) are used as a forcing fields feed through atmospheric data model. In addition, river inflow of freshwater and nutrient deposition from 71 main rivers is processed by land model. At present, satellite data from AQUA MODIS, processed by the SatBałtyk project Operational System are used for the assimilation of sea surface temperature and chlorophyll a concentration. In the operational mode, 48-hour forecasts are produced at six-hour intervals, providing a wide range of hydrodynamic and biochemical parameters.

Abstract. Coherent variation in CaCO3 burial is a feature of the Cenozoic eastern equatorial Pacific. Nevertheless, there has been a long-standing ambiguity in whether changes in CaCO3 dissolution or changes in equatorial primary production might cause the variability. Since productivity and dissolution leave distinctive regional signals, a regional synthesis of data using updated age models and high-resolution stratigraphic correlation is an important constraint to distinguish between dissolution and production as factors that cause low CaCO3. Furthermore, the new chronostratigraphy is an important foundation for future paleoceanographic studies. The ability to distinguish between primary production and dissolution is also important to establish a regional carbonate compensation depth (CCD). We report late Miocene to Holocene time series of XRF-derived (X-ray fluorescence) bulk sediment composition and mass accumulation rates (MARs) from eastern equatorial Pacific Integrated Ocean Drilling Program (IODP) sites U1335, U1337, and U1338 and Ocean Drilling Program (ODP) site 849, and we also report bulk-density-derived CaCO3 MARs at ODP sites 848, 850, and 851. We use physical properties, XRF bulk chemical scans, and images along with available chronostratigraphy to intercorrelate records in depth space. We then apply a new equatorial Pacific age model to create correlated age records for the last 8 Myr with resolutions of 1–2 kyr. Large magnitude changes in CaCO3 and bio-SiO2 (biogenic opal) MARs occurred within that time period but clay deposition has remained relatively constant, indicating that changes in Fe deposition from dust is only a secondary feedback to equatorial productivity. Because clay deposition is relatively constant, ratios of CaCO3 % or biogenic SiO2 % to clay emulate changes in biogenic MAR. We define five major Pliocene–Pleistocene low CaCO3 % (PPLC) intervals since 5.3 Ma. Two were caused primarily by high bio-SiO2 burial that diluted CaCO3 (PPLC-2, 1685–2135 ka, and PPLC-5, 4465–4737 ka), while three were caused by enhanced dissolution of CaCO3 (PPLC-1, 51–402 ka, PPLC-3, 2248–2684 ka, and PPLC-4, 2915–4093 ka). Regional patterns of CaCO3 % minima can distinguish between low CaCO3 caused by high diatom bio-SiO2 dilution versus lows caused by high CaCO3 dissolution. CaCO3 dissolution can be confirmed through scanning XRF measurements of Ba. High diatom production causes lowest CaCO3 % within the equatorial high productivity zone, while higher dissolution causes lowest CaCO3 percent at higher latitudes where CaCO3 production is lower. The two diatom production intervals, PPLC-2 and PPLC-5, have different geographic footprints from each other because of regional changes in eastern Pacific nutrient storage after the closure of the Central American Seaway. Because of the regional variability in carbonate production and sedimentation, the carbonate compensation depth (CCD) approach is only useful to examine large changes in CaCO3 dissolution.

The University of Natural Resources and Life Sciences (BOKU) in Vienna (Austria) in cooperation with the National Drought Management Authority (NDMA) in Nairobi (Kenya) has setup an operational processing chain for mapping drought occurrence and strength for the territory of Kenya using the Moderate Resolution Imaging Spectroradiometer (MODIS) NDVI at 250 m ground resolution from 2000 onwards. The processing chain employs a modified Whittaker smoother providing consistent NDVI “Mondayimages” in near real-time (NRT) at a 7-daily updating interval. The approach constrains temporally extrapolated NDVI values based on reasonable temporal NDVI paths. Contrary to other competing approaches, the processing chain provides a modelled uncertainty range for each pixel and time step. The uncertainties are calculated by a hindcast analysis of the NRT products against an “optimum” filtering. To detect droughts, the vegetation condition index (VCI) is calculated at pixel level and is spatially aggregated to administrative units. Starting from weekly temporal resolution, the indicator is also aggregated for 1- and 3-monthly intervals considering available uncertainty information. Analysts at NDMA use the spatially/temporally aggregated VCI and basic image products for their monthly bulletins. Based on the provided bio-physical indicators as well as a number of socio-economic indicators, contingency funds are released by NDMA to sustain counties in drought conditions. The paper shows the successful application of the products within NDMA by providing a retrospective analysis applied to droughts in 2006, 2009 and 2011. Some comparisons with alternative products (e.g. FEWS NET, the Famine Early Warning Systems Network) highlight main differences.

Abstract. Soil organic N is largely composed of inherently biologically labile proteinaceous N and its persistence in soil is mainly explained by stabilization through binding to minerals and other soil organic matter (SOM) components at varying strengths. In order to separate kinetically different soil N fractions we hypothesize that an approach, which isolates soil N fractions on the basis of bonding strength is required, rather than employing chemical agents or physical methods. We developed a sequential subcritical water extraction (SCWE) procedure at 100 °C, 150 °C and 200°C to isolate SOM fractions. We assessed these SCWE N fractions as predictors for aerobic and anaerobic N mineralization measured from 25 paddy soil cores in incubations. SCWE organic carbon (SCWE OC) and N (SCWE N) increased exponentially with the increase of temperature and N was extracted preferentially over OC. The efficiency of SCWE and the selectivity towards N were both lower in soils with increasingly reactive clay mineralogy. The 100–150 °C SCWE N and the 100 °C + 100–150 °C SCWE N correlated slightly better with the aerobic N mineralization than soil N content. The resulting explained percentages (46–49%) are, however, too small to allow accurate fertilizer recommendations. No correlations were found between the SCWE fractions and anaerobic N mineralization rate and consequently alternative driving factors should be looked for. Nonetheless, perhaps SCWE does, still hold potential to separate kinetically different SOM pools from upland soils, in which the bio-availability of N is more likely to be a key constraint in the N mineralization process. This in contrast to the studied paddy soils, in which N mineralization appeared to be largely decoupled from SOM quantity and quality or its availability, perhaps due to an overriding control of abiotic factors on the anaerobic N mineralization process.

For the 21st century, scenarios of future climate under global warming suggest that Bassian-Eyrean bioclimatic region of northern Victoria, centred on the North Central Catchment Management Authority (NCCMA), will become markedly warmer and drier. Significant climate change is a real possibility midcentury and some basic bio-physical attributes underpinning the current ecology, land-use and management will be altered. Societal adaptation to climate change will include enhancing landscape resilience and changes to the mix of inter-related ecosystem services. The increasing understanding of these inter-relationships will allow for the creation of a more holistic quantification and production of landscape services. In combination, these challenge the past land-use paradigm on the driest, inhabited continent. Following the mid-19th century gold rushes, land-use in the NCCMA represented the epitome of the colonial land-use paradigm through clearing for agriculture and pastoralism. Victoria has long had the highest percentage private land of any Australian state. The NCCMA catchment is the most denuded of native vegetation, with the smallest percentage of public land and conservation reserves, and is now the centre of a continental concentration of bioregions under high environmental stress. The original primacy of agriculture was fulfilled, sometimes under adverse circumstances, but resultant landscape legacies persist within the relative economic decline of Australian agriculture. The amelioration of these within a future land stewardship that is water-stressed, carbon constrained and prone to extreme weather events is a major challenge. Exploring landscape adaptation, the simple questions arise: From what? To what? This contribution examines broad land-use in the NCCMA in the long term context of climate change and adaptation, land-use and the perceived valuation of ecosystem services from the landscape. The increasing realisation of the interconnectedness of these phenomena and the necessity for ecologically sustainable agriculture provide enhanced drivers for the evolution of new landscape meanings in the context of an inter-generational equity and climate change response.

Abstract. As aerosol amount and type are key factors in the atmospheric correction required for remote-sensing chlorophyll a concentration (Chl) retrievals, the Multi-angle Imaging SpectroRadiometer (MISR) can contribute to ocean color analysis despite a lack of spectral channels optimized for this application. Conversely, an improved ocean surface constraint should also improve MISR aerosol-type products, especially spectral single-scattering albedo (SSA) retrievals. We introduce a coupled, self-consistent retrieval of Chl together with aerosol over dark water. There are time-varying MISR radiometric calibration errors that significantly affect key spectral reflectance ratios used in the retrievals. Therefore, we also develop and apply new calibration corrections to the MISR top-of-atmosphere (TOA) reflectance data, based on comparisons with coincident MODIS (Moderate Resolution Imaging Spectroradiometer) observations and trend analysis of the MISR TOA bidirectional reflectance factors (BRFs) over three pseudo-invariant desert sites. We run the MISR research retrieval algorithm (RA) with the corrected MISR reflectances to generate MISR-retrieved Chl and compare the MISR Chl values to a set of 49 coincident SeaBASS (SeaWiFS Bio-optical Archive and Storage System) in situ observations. Where Chlin situ < 1.5 mg m−3, the results from our Chl model are expected to be of highest quality, due to algorithmic assumption validity. Comparing MISR RA Chl to the 49 coincident SeaBASS observations, we report a correlation coefficient (r) of 0.86, a root-mean-square error (RMSE) of 0.25, and a median absolute error (MAE) of 0.10. Statistically, a two-sample Kolmogorov–Smirnov test indicates that it is not possible to distinguish between MISR Chl and available SeaBASS in situ Chl values (p > 0.1). We also compare MODIS–Terra and MISR RA Chl statistically, over much broader regions. With about 1.5 million MISR–MODIS collocations having MODIS Chl < 1.5 mg m−3, MISR and MODIS show very good agreement: r = 0. 96, MAE = 0.09, and RMSE = 0.15. The new dark water aerosol/Chl RA can retrieve Chl in low-Chl, case I waters, independent of other imagers such as MODIS, via a largely physical algorithm, compared to the commonly applied statistical ones. At a minimum, MISR's multi-angle data should help reduce uncertainties in the MODIS–Terra ocean color retrieval where coincident measurements are made, while also allowing for a more robust retrieval of particle properties such as spectral single-scattering albedo.

Abstract. The deposition of atmospheric dust is the primary process supplying trace elements abundant in crustal rocks (e.g. Al, Mn and Fe) to the surface ocean. Upon deposition, the residence time in surface waters for each of these elements differs according to their chemical speciation and biological utilization. Presently, however, the chemical and physical processes occurring after atmospheric deposition are poorly constrained, principally because of the difficulty in following natural dust events in situ. In the present work we examined the temporal changes in the biogeochemistry of crustal metals (in particular Al, Mn and Fe) after an artificial dust deposition event. The experiment was contained inside trace metal clean mesocosms (0–12.5 m depths) deployed in the surface waters of the northwestern Mediterranean, close to the coast of Corsica within the frame of the DUNE project (a DUst experiment in a low Nutrient, low chlorophyll Ecosystem). Two consecutive artificial dust deposition events, each mimicking a wet deposition of 10 g m−2 of dust, were performed during the course of this DUNE-2 experiment. The changes in dissolved manganese (Mn), iron (Fe) and aluminum (Al) concentrations were followed immediately after the seeding with dust and over the following week. The Mn, Fe and Al inventories and loss or dissolution rates were determined. The evolution of the inventories after the two consecutive additions of dust showed distinct behaviors for dissolved Mn, Al and Fe. Even though the mixing conditions differed from one seeding to the other, Mn and Al showed clear increases directly after both seedings due to dissolution processes. Three days after the dust additions, Al concentrations decreased as a consequence of scavenging on sinking particles. Al appeared to be highly affected by the concentrations of biogenic particles, with an order of magnitude difference in its loss rates related to the increase of biomass after the addition of dust. In the case of dissolved Fe, it appears that the first dust addition resulted in a decrease as it was scavenged by sinking dust particles, whereas the second seeding induced dissolution of Fe from the dust particles due to the excess Fe binding ligand concentrations present at that time. This difference, which might be related to a change in Fe binding ligand concentration in the mesocosms, highlights the complex processes that control the solubility of Fe. Based on the inventories at the mesocosm scale, the estimations of the fractional solubility of metals from dust particles in seawater were 1.44 &amp;pm; 0.19% and 0.91 ± 0.83% for Al and 41 ± 9% and 27 ± 19% for Mn for the first and the second dust addition. These values are in good agreement with laboratory-based estimates. For Fe no fractional solubility was obtained after the first seeding, but 0.12 ± 0.03% was estimated after the second seeding. Overall, the trace metal dataset presented here makes a significant contribution to enhancing our knowledge on the processes influencing trace metal release from Saharan dust and the subsequent processes of bio-uptake and scavenging in a low nutrient, low chlorophyll area.

Abstract This paper summarizes a six-year study of the Nsimi Small Experimental Watershed (SEW), considered as a model for the South Cameroon humid tropical ecosystem. When this small watershed was set up, no similar survey of input/output hydrobiogeochemical fluxes in granitoid rocks in stable cratonic environment was available, to our knowledge, on any site close to the Equator. Moreover, this is the first attempt, world-wide, to combine different approaches in hydrology, (bio)geochemistry, mineralogy, crystallography, microbiology, geophysics and pedology. Research is based on (1) regular hydrobiogeochemical surveys in various water reservoirs of the SEW ecosystem (atmospheric deposits, groundwater and stream), (2) surveys related either to the organisation and composition of different reservoirs in the superficial layers (basement rocks, saprolite, soils) or to various hydrological, biological and geochemical processes. These surveys aim at (1) finding the main parameters involved in the chemical and physical erosion processes of the humid tropical ecosystem, (2) understanding the source of a particular chemical composition in groundwater and rivers, (3) documenting accurately the different exportation processes of chemical elements in water and soil (4) investigating the possible relation between the biodegradation of soil organic matter and the leaching of metals (especially iron) and (5) comparing the long and short term weathering rates using mass balance calculations. Another important objective of this study is to provide a new scientific and engineering database for the future development of South Cameroon, which is still nowadays a relatively preserved ecosystem. One of the major results is the essential role played by the biological cycle (vegetation and soil organic matter) in the fractionation, exportation or storage of the chemical elements in humid tropical environments. Moreover we are able to propose a model of the current erosion for this SEW from the database obtained on (1) the mineralogy of the basement rocks and the soil layers, (2) the geochemistry of the soluble and colloidal phases of waters and (3) the hydrology within the different reservoirs of the hydrosystem. This model has been confirmed and extended on a regional scale (Nyong river basin). It emphasized the behaviour of the main elements of the tropical soil layers (Fe, Al, Si), the nutrients (C, Ca, Mg, K, Sr) and specific tracers of the weathering processes either with strong mobility (Cl, Na) or on the contrary with an extremely low mobility (Zr, Th, REEs). On the SEW scale, a strong geochemical contrast occurs between the different groundwater zones flooding (1) the hill slope lateritic profiles, (2) the weathering front (interface between the saprolite and the basement rocks), and (3) the swampy zone in which the Mengong brook flows. High DOC contents (15 mg/L) but also high Fe, Th, Al, Zr contents characterize the swampy zone waters. Na and Si have mainly a deep origin (exfiltration), Al, Th, Zr and REEs are strongly linked with colloidal organic matter located in the upper horizons of the swamp. Fe has a much more complex behaviour due to its change of redox state which can be independent of organic matter complexation. Concerning the major base cations, their origin can be constrained by the biological cycle (storage or leaching). K is typically influenced by the biological cycle. During the floods, Cl has the same behaviour as K: it is one of the most striking points of this study. However, the Cl annual budget is balanced. These characteristics can be understood as the consequence of the weathering of the minerals present in the saprolite (kaolinite, goethite, zircon, Th-oxide). This chemical weathering allows the leaching of base cations and also Al and Fe. It has been demonstrated that the microbial populations of the swampy zone can play an important role in the mobilization of transition metals (e.g. Fe). This study point out the role of humic acids in the transport and the weathering budget of elements usually considered as immobile in the superficial cycle (e.g. Al, Th, Zr, Fe). It must be mentioned that worldwide the SEW and even the Nyong network waters are among the least concentrated river waters. It means that even if the organic matter plays an important role in the mobilization and transport of some elements in the swampy zone, its action is limited in term of major cation fluxes on the SEW scale. The reason invoked is that the cation fluxes are directly linked to the pedological history and the geomorphology of the watershed. The presence of thick soil layers composed of saprolite and latosol on the hillsides and of hydromorphic soils in the swampy zone with constant mineralogy lead to isolating the bedrock. The long residence time of water close to the weathering front plays a major role in preserving the parent rock from the hydro-chemical outputs. Moreover, the topsoil layers are stabilized by the vegetation cover, which limits mechanical erosion. This should be taken into account for the carbon mass balance calculation because of the wide areas on stable shields concerned by the humid tropical ecosystems. Moreover, comparison between long and short-term weathering allows us to suggest that paleo-climatic conditions did not change since the Miocene (6–20 Ma) in this part of the world.

Seven spot ladybird beetle, (Coccinella septempunctata) is a widely distributed natural enemy of soft-bodied insect pests especially aphids worldwide. Both the adult and larvae of this coccinellid beetle are voracious feeders and serve as a commercially available biological control agent around the globe. Different techniques are adopted to enhance the mass rearing and storage of this natural enemy by taking advantage of its natural ability to withstand under extremely low temperatures and entering diapause under unfavorable low temperature conditions. The key objective of this study was to develop a cost effective technique for enhancing the storage life and predatory potential of the larvae of C. septempunctata through cold storage in conjunction with the use of nuclear techniques, gamma radiations. Results showed that the host eating potential of larvae was enhanced as the cold storage duration was increased. Gamma irradiation further enhanced the feeding potential of larvae that were kept under cold storage. Different irradiation doses also affected the development time of C. septempuntata larvae significantly. Without cold storage, the lower radiation doses (10 and 25 GY) prolonged the developmental time as compared to un-irradiated larvae. Furthermore, the higher dose of radiation (50GY) increased the developmental time after removal from cold storage. This study first time paves the way to use radiation in conjunction with cold storage as an effective technique in implementation of different biological control approaches as a part of any IPM programs.Key wordGamma irradiations; cold storage, Coccinella septempunctata larvae; predatory potential; integrated pest management programme.INTRODUCTIONNuclear techniques such as gamma radiations have a vast application in different programmes of biological control including continuous supply of sterilized host and improved rearing techniques (Greany and Carpenter, 2000; Cai et al., 2017). Similarly irradiation can be used for sentinel-host eggs and larvae for monitoring survival and distribution of parasitoids (Jordão-paranhos et al., 2003; Hendrichs et al., 2009; Tunçbilek et al., 2009; Zapater et al., 2009; Van Lenteren, 2012). Also, at the production level, such technique may facilitate the management of host rearing, improve quality and expedite transport of product (Fatima et al., 2009; Hamed et al., 2009; Wang et al., 2009). Gamma irradiations can also be used to stop insect’s development to enhance host suitability for their use in different mass rearing programs (Celmer-Warda, 2004; Hendrichs et al., 2009; Seth et al., 2009). Development and survival of all insects have a direct connection with temperatures which in turn affect the physical, functional and behavioral adaptations (Ramløy, 2000). Many insects living in moderate regions can survive at low temperature by process of diapause. A temperature between 0 to 10oC may cause some insects to become sluggish and they only become active when the temperature is suitable. Such insects show greater adaptations to flexible temperature regimes for better survival. Many studies have reported this concept of cold-hardiness in insects in general (Bale, 2002; Danks, 2006) and specifically in coccinellid beetles over past years (Watanabe, 2002; Koch et al., 2004; Pervez and Omkar, 2006; Labrie et al., 2008; Berkvens et al., 2010). Using this cold hardiness phenomenon, many coccinellids have been studied for the effect of cold storage such as Coccinella undecimpunctata (Abdel‐Salam and Abdel‐Baky, 2000), Coleomegilla maculata (Gagné and Coderre, 2001) and Harmonia axyridis (Watanabe, 2002). This natural phenomenon, therefore, can be a helpful tool in developing low temperature stockpiling for improving mass-rearing procedures (Mousapour et al., 2014). It may provide a significant output in terms of providing natural enemies as and when required during pest infestation peaks (Venkatesan et al., 2000). Use of irradiation in conjunction with cold storage proves to be an effective technique in implementation of different biological control approaches as a part of any IPM programme. A study reported that the pupate of house fly, Musca domestica irradiated at dose of 500 Gy and can stored up to 2 months at 6°C for future use for a parasitoid wasp Spalangia endius rearing (Zapater et al., 2009). Similarly, when irradiated at 20 GY, parasitic wasps Cotesia flavipes were stored safely up to two months without deterioration of their parasitic potential (Fatima et al., 2009). Similarly, bio-control program of sugarcane shoot borer Chilo infescatellus proved successful through the use of irradiation combined with cold storage of its egg and larval parasitoids Trichogramma chilonis and C. flavipes (Fatima et al., 2009). Less mobile life stages such as larvae are of significance in any IPM strategy because they remain on target site for more time period as compared to adults. Therefore, use of predatory larvae is very promising in different biological control approaches because of their immediate attack on pests and more resistance to unfavorable environmental conditions than delicate egg stage. In addition, with their augmentation into fields, larval stage shows their presence for longer time than adult stage and their feeding potential is also satisfactory as that of adults. For the best utilization of these predators in the field and maximum impact of 3rd and 4th larval instars on prey, we should encourage late 2nd second instar larvae of predatory beetles in the fields as these instars have more feeding capacity due to increased size and ability to handle larger preys.In spite of higher significance, there is little information available about the effect of cold storage on the survival of larval instars of different ladybird beetles and its effect on their predatory potential. Very few studies report the use of cold storage for non-diapausing larval stage like for Semiadalia undecimnotata and only one study reported the short-term storage (up to two weeks) of 2nd and 3rd instar coccinellid, C. maculate, without any loss in feeding voracity of larvae after storage (Gagné and Coderre, 2001). The survival of 3rd and 4th larval instars of C. undecimpunctata for 7 days after storage at 5oC was reported in a study but the survival rate declined after 15-60 days of storage (Abdel‐Salam and Abdel‐Baky, 2000). As C. septempunctata is considered one of the voracious predators (Afroz, 2001; Jandial and Malik, 2006; Bilashini and Singh, 2009; Xia et al., 2018) and diapause is a prominent feature of this beetle and it may undergo facultative diapause under suitable laboratory conditions (Suleman, 2015). No information is available to date about the combined effect of cold storage and irradiation on the larval instars of this species.OBJECTIVES The objective of this study was to devise a cost effective technique for the cold storage and its effect on the subsequent predatory potential of the seven spotted ladybird beetle larvae in conjunction with the use of gamma radiations. Hypothesis of the study was that an optimum length of low temperature treatment for storage purpose would not affect the predation capacity of C. septempunctata larvae and their developmental parameters including survival and pupation will remain unaffected. Furthermore, use of gamma irradiation will have some additional effects on survival and feeding capacity of irradiated C. septempunctata larvae. Such techniques can be utilized in different biocontrol programs where short term storage is required. So these larvae can be successfully imparted in different IPM programs against sucking complex of insect pests as a component of biological control strategyMATERIALS AND METHODSPlant materials: Collection and rearing of C. septempunctata: Adult C. septempunctata were collected from the wheat crop (in NIAB vicinity and farm area) in the month of March during late winter and early in spring season 2016-2017. They were kept in plastic jars and were fed with brassica aphids. Under controlled laboratory conditions (25+2oC, 16h: 8h L:D and 65+5% R.H.), eggs of C. septempuctata were obtained and after hatching, larvae were also given brassica aphids as dietary source. Larvae of second instar were selected for this experiment (as the first instar is generally very weak and vulnerable to mortality under low temperatures). As the larvae approached second instar, they were separated for the experimentation. Irradiation of larvae at different doses: Irradiation of larvae was carried out by the irradiation source 137CS at Radiation laboratory, and the larvae were then brought back to the IPM laboratory, Plant Protection Division, Nuclear Institute for Agriculture and Biology (NIAB) Faisalabad. Radiation doses of 10 GY (Grey), 25 GY and 50 GY were used to treat the second instar larvae. There were three replicates for each treatment and five larvae per replicate were used. Control treatment was left un-irradiated.Cold storage of irradiated larvae: In present work, second instar C. septempunctata larvae were studied for storage at low temperature of 8oC. The larvae were kept at 8oC for 0, I and II weeks where week 0 depicts no cold treatment and this set of larvae was left under laboratory conditions for feeding and to complete their development. For larvae that were kept under cold storage for one week at 8°C, the term week I was devised. Similarly, week II denotes the larvae that remained under cold conditions (8°C) for two continuous weeks. Larvae were removed from cold storage in their respective week i.e., after week I and week II and were left under laboratory conditions to complete their development by feeding on aphids. Data collection: For recording the predatory potential of C. septempunctata larvae, 100 aphids were provided per larva per replicate on a daily basis until pupation as this number was more than their feeding capacity to make sure that they were not starved (personal observation). Observations were recorded for survival rate, developmental time and feeding potential. Data analysis: Data were statistically analysed by Statistical Software SPSS (Version 16.0). The data were subjected to normality check through the One-sample Kolmogorov-Smirnov test. Non normal data were transformed to normal data which were then used for all parametric variance tests. One-way and two-way analyses of variance were used. For comparison between variables, LSD test at α 0.05 was applied.RESULTSFeeding potential of irradiated larvae after removal from cold storage: Results showed an increase in the feeding potential of C. septempunctata larvae with increased cold storage duration. The feeding potential was significantly higher for the larvae that spent maximum length of time (week II) under cold storage conditions followed by week I and week 0. Gamma irradiations further enhanced the feeding potential of larvae that were kept under cold storage. When larvae were irradiated at 10 GY, the eating capacity of larvae increased significantly with the duration of cold storage. Similarly, larvae that were irradiated at 25 GY, showed increase in feeding potential on aphids as the time period of cold storage increased. The feeding potential of larvae that were irradiated at 50 GY, was again significantly increased with increase of cold storage duration. When different radiation doses were compared to week 0 of storage, there was a significant difference in feeding potential and larvae irradiated at 50 GY consumed the maximum numbers of aphids when no cold storage was done followed by larvae irradiated at 10 and 25 GY. With the other treatment, where larvae were kept under cold storage for one week (week I) the larvae irradiated at 50GY again showed the highest feeding potential. The feeding potential of irradiated larvae was again significantly higher than the un-irradiated larvae that were kept for two weeks (week II) under cold storage (table 1).Two-way ANOVA was performed to check the interaction between the different radiation doses and different lengths of storage durations for feeding potential of C. septempunctata larvae on aphids. The feeding potential of larvae irradiated at different doses and subjected to variable durations of cold storage were significantly different for both the radiation doses and cold storage intervals. Furthermore, the interaction between the radiation doses and storage duration was also significant meaning that the larvae irradiated at different doses with different length of cold storage were having significant variations in feeding levels (table 2).Developmental time of irradiated larvae after removal from cold storage: Significant difference was found in the development time of the larvae of C. septempunctata when irradiated at different doses at week 0 (without cold storage). The larvae irradiated at 10 GY took the maximum time for development and with the increase in irradiation dosage, from 25 to 50 GY, the time of development was shortened. The larvae irradiated at 50 GY had the same development time as the un-irradiated ones. When, the irradiated larvae were subjected to cold storage of one week duration (week I), their development time after removal from storage condition varied significantly. The larvae irradiated at 25 GY took the maximum time for development followed by larvae irradiated at 50 GY and 10 GY. There was an indication that the development time was extended for irradiated larvae as compared to un-irradiated larvae.Results also depicted a significant difference in the time taken by irradiated larvae to complete their development after taken out from cold storage of two weeks duration (week II). As the storage time of irradiated larvae increased, the development time was prolonged. Results showed that the larvae that were irradiated at 25 and 50 GY, took the maximum time to complete their development. With the prolonged duration of cold storage up to two weeks (week II), this difference of development time was less evident at lower doses (10 GY). The larvae irradiated at 10 GY showed a significant difference in their developmental duration after being taken out of cold storage conditions of the week 0, I and II. There was no difference in the developmental duration of larvae that were un-irradiated and subjected to different regimes of storage. Un-irradiated larvae were least affected by the duration of storage. With the increase in the storage time, a decrease in the developmental time was recorded. Larvae that were irradiated at 10 GY, took the maximum period to complete their development when no cold storage was done (week 0) followed by week I and II of cold storage. When the larvae irradiated at 25 GY were compared for their development time, there was again significant difference for week 0, I and II of storage duration. Maximum time was taken by the larvae for their complete development when removed from cold storage after one week (week I). With the increase in storage duration the time taken by larvae to complete their development after removal from cold storage reduced.When the larvae were removed after different lengths of cold storage duration i.e., week 0, week I and week II, there was a significant difference in the developmental time afterwards. Results have shown that the higher dose of radiation, increased the developmental time after removal from cold storage. The larvae irradiated at 50 GY took the longest time to complete their development after removal from cold storage (week I and week II) as compared the larvae that were not kept under cold storage conditions (week 0) (table 3).Interaction between the different radiation doses and different lengths of storage durations for development time of larvae were checked by two-way ANOVA. The development time of larvae irradiated at different doses and subjected to variable durations of cold storage were significantly different for both the doses and cold storage intervals. Furthermore, the interaction between the radiation doses and storage duration was also significant meaning that the larvae irradiated at different doses with different length of cold storage were having significant variations in development times (table 4). DISCUSSIONThe present research work indicates the possibility of keeping the larval instars of C. septempunctata under cold storage conditions of 8oC for a short duration of around 14 days without affecting its further development and feeding potential. Furthermore, irradiation can enhance the feeding potential and increase the development time of larval instars. This in turn could be a useful technique in mass rearing and field release programmes for biological control through larval instars. Usually temperature range of 8-10oC is an optimal selection of low temperature for storage as reported earlier for eggs two spotted ladybird beetle, Adalia bipunctata and the eggs of C. septempunctata (Hamalainen and Markkula, 1977), Trichogramma species (Jalali and Singh, 1992) and fairyfly, Gonatocerus ashmeadi (Hymenoptra; Mymaridae) (Leopold and Chen, 2007). However, a study reported more than 80% survival rate for the coccinellid beetle, Harmonia axyridis for up to 150 days at moderately low temperature of 3-6oC (Ruan et al., 2012). So there is great flexibility in coccinellid adults and larvae for tolerating low temperature conditions. After removal from cold storage, larvae showed better feeding potential with consumption of more aphids when compared to normal larvae that were not placed under low temperature conditions. This indicates that when the adult or immature insect stages are subjected to low temperature environment, they tend to reduce their metabolic activity for keeping them alive on the reserves of their body fats and sustain themselves for a substantial length of time under such cold environment. Hereafter, the larval instars that were in cold storage were behaving as if starved for a certain length of time and showed more hunger. This behavior of improved or higher feeding potential of stored larvae has been reported previously (Chapman, 1998). Hence, the feeding potential of C. septempunctata larvae significantly increased after cold storage. Gagné and Coderre (2001) reported higher predatory efficacy in larvae of C. maculata when stored at the same temperature as in the present study i.e., 8oC. Similarly, Ruan et al. (2012) showed that the multicolored Asian ladybug, H. axyridis, when stored under cold conditions, had more eating capacity towards aphids Aphis craccivora Koch than the individuals that were not stored. Such studies indicate that the higher feeding potential in insects after being subjected to low temperature environmental conditions could be due to the maintenance of their metabolism rate to a certain level while utilizing their energy reserves to the maximum extent (Watanabe, 2002).The individuals coming out from cold storage are therefore capable of consuming more pray as they were in a condition of starvation and they have to regain their energy loss through enhanced consumption. Furthermore, the starvation in C. septempunctata has previously been reported to affect their feeding potential (Suleman et al., 2017). In the present study, the larval development was delayed after returning to normal laboratory conditions. Cold storage affects the life cycle of many insects other than coccinellids. The cold storage of green bug aphid parasitoid, Lysiphlebus testaceipes Cresson (Hymenoptra; Braconidae) mummies increased the life cycle 3-4 times. Nevertheless, in current study the development process of stored larvae resumed quickly after taking them out and larvae completed their development up to adult stage. Similar kinds of results were reported for resumption of larval development after removal from cold storage conditions. Such studies only report satisfactory survival rates and development for a short duration of cold storage but as the length of storage is increased, it could become harmful to certain insects. Gagné and Coderre (2001) reported that cold storage for longer period (three weeks) proved fatal for almost 40% of larvae of C. maculata. Furthermore, in the same study, the feeding potential of C. maculata larvae was also affected beyond two weeks of cold storage due to the loss of mobility after a long storage period. Many studies have reported that longer durations of low temperature conditions can either damage the metabolic pathways of body cells or may increase the levels of toxins within the bodies of insects. Also, low temperature exposure for longer duration may cause specific interruptions in the insect body especially neuro-hormones responsible for insect development, which could be dangerous or even life threatening.Chen et al. (2004) also reported that the biological qualities of parasitized Bemisia tabaci pupae on population quality of Encarsia formosa were affected negatively with increase in cold storage duration. Similarly, the egg hatchability of green lacewing Chrysoperla carnea Stephen was lost completely beyond 18 days of cold storage (Sohail et al., 2019). However, in the present study the cold storage was done for maximum two weeks and it is to be regarded as a short term storage hence the survival rate was satisfactory. Longer periods of cold storage for larvae are not considered safe due to their vulnerable state as compared to adults which are hardier. Also 2nd instar larvae used in the present study for cold storage for being bigger in size and physical stronger than 1st instar. Abdel‐Salam and Abdel‐Baky (2000) reported that in C. undecimpunctata the cold storage of 3rd and 4th larval instars was higher and considered safer than early larval instars. The same study showed sharp decline in survival rate after two weeks and there was no survival beyond 30-60 days of cold storage. The present study showed that short term storage of the larvae of C. septempunctata could be done without any loss of their feeding potential or development so the quality of predator remained unaffected. Similar kind of work for many other insects had been reported previously where cold storage technique proved useful without deteriorating the fitness of stored insects. For example, the flight ability of reared codling moth Cydia pomonella Linnaeus remained unaffected after removal from cold storage (Matveev et al., 2017). Moreover, a sturdy reported that pupae of a parasitoid wasp Trichogramma nerudai (Hymenoptera; Trichogrammatidae) could be safely put in cold storage for above than 50 days (Tezze and Botto, 2004). Similarly, a technique of cold storage of non-diapausing eggs of black fly Simulium ornaturm Meigen was developed at 1oC. Another study reported safe storage of a predatory bug insidious flower bug Orius insidiosus for more than 10 days at 8°C (Bueno et al., 2014).In present study without cold storage, the lower doses of 10 and 25 GY prolonged the developmental time as compared to un-irradiated larvae and higher doses of irradiations in conjunction with cold storage again significantly prolonged the developmental time of larvae when returned to the laboratory conditions. Salem et al. (2014) also reported that Gamma irradiations significantly increased the duration of developmental stages (larvae and pupae) in cutworm, Agrotis ipsilon (Hufnagel). In another study, where endoparasitic wasps Glyptapanteles liparidis were evaluated with irradiated and non-irradiated gypsy moth Lymantria dispar larvae for oviposition, it was found that non-irradiated larvae had a shorter time to reach the adult stage as compared to irradiated larvae (Novotny et al., 2003). Both for higher doses with cold storage and lower doses without cold storage extended the larval duration of C. septempunctata. In another study when the parasitoid wasp Habrobracon hebetor was irradiated at the dose of 10 GY, it resulted in prolonged longevity (Genchev et al., 2008). In the same study, when another parasitoid Ventruria canescens was irradiated at lower doses of 4GY and 3 GY, it resulted in increased emergence from the host larvae, while gamma irradiations at the dose of 1 GY and 2 GY significantly stimulated the rate of parasitism (Genchev et al., 2008). The current study also indicated higher rates of predation in the form of increased feeding potential of larvae as a result of irradiations at lower doses.CONCLUSIONThe outcome of the current study shows that storage of 2nd instar C. septempunctata at low temperature of 8oC for a short duration of about 14 days is completely safe and could have broader application in different biocontrol programs. Such flexibility in storage duration can also assist in different mass rearing techniques and commercial uses. The combination of gamma radiation with low temperature cold storage could be a useful tool in developing different biological pest management programs against sucking insect pests. Incidence of periodic occurrence of both the target insect pests with their predatory ladybird beetles in synchrony is an important aspect that could be further strengthened by cold storage techniques. Therefore, short or long term bulk cold storage of useful commercial biocontrol agents and then reactivating them at appropriate time of pest infestation is a simple but an advantageous method in mass rearing programs. Increased feeding capacity of stored larvae is another edge and hence such larvae may prove more beneficial as compared to unstored larvae. Both cold storage and improved feeding of the C. septempuctata larvae can be utilized for implementation of IPM for many sucking insect pests of various crops, fruits and vegetables. Due to some constraints this study could not be continued beyond two weeks but for future directions, higher doses and longer duration periods could further elaborate the understanding and better application of such useful techniques in future IPM programmes on a wider scale. Also, some other predatory coccinellid beetle species can be tested with similar doses and cold storage treatments to see how effective this technique is on other species as well.ACKNOWLEDGMENTS We acknowledge the Sugarcane Research and Development Board for providing a research grant (No. SRDB/P/4/16) to carry out this research work. 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This study aimed to assess the constraints faced by rice farmers under RKVY, their suggestions and a strategy was developed to overcome the constraints. The present study was conducted in 3 districts viz Srikakulam, Nellore and West Godavari of Andhra Pradesh. Ex post facto research design was used and data was collected through interview schedule from 240 farmers. Collected data is analyzed using Garett test and other suitable statistical tools. Multiple responses were considered to ascertain the constraints faced by the rice growers in adoption of recommended technologies of Acharya NG Ranga Agricultural University (ANGRAU). The constraints faced by the rice growers in adoption of recommended technologies are classified into 5 categories viz., Personnel constraints, Bio physical constraints, Socio-Economic Constraints, Marketing constraints and Organizational constraints. The major constraints perceived by the farmers among different categories were Labour scarcity & high wage rates during peak periods, less mass media exposure, interference of middlemen and Epidemics of pest & diseases. The suggestions given by the farmers were prioritization of agricultural activities in MGNREGA scheme, Conduct of hands on experience training programs on usage of ICT tools and social media for browsing information related to agriculture, Setting up of sufficient number of public purchase points at local level by the government, Conducting sufficient number of practical oriented season long training programs during crop period and Provision of timely updates regarding fluctuations in market prices. A suitable strategy was evolved addressing the various constraints which would be helpful for the policy makers, researchers, extension functionaries to plan and modify the extension programs so as to mitigate these constraints faced by the rice farmers and thereby to augment rice production.

Air quality monitoring nowadays has become a vital role to assess air quality level, to ensure the health of community are not affected with poor air quality. However, approaches for air quality monitoring either via physical or chemical assessment has built up some constraints. Time consuming, inaccuracy of data due to high maintenance of monitoring devices and only require competent person that able to conduct the monitoring. Therefore, the demand of using bio-indicator, such as moss, for air quality monitoring has risen due to multiple advantages. In this study, moss response and survivability rate towards acidic condition has been physically observed. The color changed and moss grid area were the parameters used to observe moss after acidic treatment. The moss, Leucobryum glaucum, was treated with artificial acid rain at three different pH; pH 3, 4 and 5. The moss was treated with 25 ml of artificial acid rain, once in two days for the duration of over 10 weeks; from week 4 until week 14 during the study period. The result showed that the moss mostly reacted towards lower pH of acid rain, at pH 3. The survivability rate of moss was decreasing when treated with pH 5 to pH 3. The physical observation which is determined by the area covered by moss (per square meter), showed that most of the area that covered by moss are affected at pH 3. The fresh green color that were observed before the artificial acid rain treatment has changed to yellowish color towards the 10 week of treatment. This study suggested that moss could be used by communities as a simple bio-indicator, to monitor air quality, especially for determination of acidic condition in certain area due to any surrounding factors.

Abstract Micro-milling is a precision manufacturing process with broad applications across the biomedical, electronics, aerospace, and aeronautical industries owing to its versatility, capability, economy, and efficiency in a wide range of materials. In particular, the micro-milling process is highly suitable for very precise and accurate machining of mold prototypes with high aspect ratios in the microdomain, as well as for rapid micro-texturing and micro-patterning, which will have great importance in the near future in bio-implant manufacturing. This is particularly true for machining of typical difficult-to-machine materials commonly found in both the mold and orthopedic implant industries. However, inherent physical process constraints of machining arise as macro-milling is scaled down to the microdomain. This leads to some physical phenomena during micro-milling such as chip formation, size effect, and process instabilities. These dynamic physical process phenomena are introduced and discussed in detail. It is important to remember that these phenomena have multifactor effects during micro-milling, which must be taken into consideration to maximize the performance of the process. The most recent research on the micro-milling process inputs is discussed in detail from a process output perspective to determine how the process as a whole can be improved. Additionally, newly developed processes that combine conventional micro-milling with other technologies, which have great prospects in reducing the issues related to the physical process phenomena, are also introduced. Finally, the major applications of this versatile precision machining process are discussed with important insights into how the application range may be further broadened.

The ultra-high significances of thermal radiation, magnetic field and activation energy in thermal enhancement processes allow significant applications in chemical and mechanical engineering, modern technology and various thermal engineering eras. The improvement in energy resources and production became one of the major challenges for researchers and scientists for sustained development in industrial growths. Beside this, the bioconvection assessment in nanomaterials conveys prestigious applications in biotechnology like bio-sensors, enzymes, petroleum industry, bio-fuels and many more. In view of such renewable applications, present exploration discloses unsteady two-dimensional flow of third-grade nanomaterial accommodating gyrotactic microorganisms induced by unsteady stretched Riga sheet in porous medium. The formulated flow problem is further scrutinized by utilizing the chemical reaction, activation energy, thermal radiation and magnetic aspects. The convective Nield constraints are further subjected in the current investigation. Apposite transformations are used to condense the nonlinear developed problem into dimensionless ordinary form. The numerical solution of such similar flow problem is presented via shooting technique. The detailed graphical illustrations of the dimensionless temperature, nanoparticles concentration, velocity and motile microorganisms for physical significance of diverse relevant parameters are deliberated. Furthermore, numerical data of local Sherwood, Nusselt and motile density numbers is designated in tabular form. Study accentuated that velocity increases for higher modified Hartmann and material constants, while the effects of buoyancy ratio and bioconvected Rayleigh numbers are rather opposite. The temperature, microorganism and concentration distributions were enhanced for unsteady parameter. It is also acknowledged that the concentration distribution is enhanced for activating the energy number. Moreover, the microorganism distribution enhances for concentration difference and magneto-porous constants, while bioconvected Lewis and Peclet numbers show conflicting trend.

Abstract:The encapsulation of living cells within inorganic silica hydrogels is a promising strategy for the design of biosensors, modular bioreactors, and bioremediation devices, among other interesting applications, attracting scientific and technological interest. These hostguest multifunctional materials (HGFM) combine synergistically specific biologic functions of their guest with those of the host matrix enhancing their performance. Although inorganic immobilization hosts present several advantages over their (bio)polymer-based counterparts in terms of chemical and physical stability, the direct contact of cells with silica precursors during synthesis and the constraints imposed by the inorganic host during operating conditions have proved to influence their biological response. Recently, we proposed an alternative two-step procedure including a pre-encapsulation in biocompatible polymers such as alginates in order to confer protection to the biological guest during the inorganic and more cytotoxic synthesis. By means of this procedure, whole cultures of microorganisms remain confined in small liquid volumes generated inside the inorganic host, providing near conventional liquid culture conditions.Moreover, the fact of protecting the biological guest during the synthesis of the host, allows extending the synthesis parameters beyond biocompatible conditions, tuning the microstructure of the matrix. In turn, the microstructure (porosity at the nanoscale, radius of gyration of particles composing the structure, and fractal dimension of particle clusters) is determinant of macroscopic parameters, such as optical quality and transport properties that govern the encapsulation material’s performance. Here, we review the most interesting applications of the two-step procedure, making special emphasis on the optimization of optical, transport and mechanical properties of the host as well as in the interaction with the guest during operation conditions.

This study presents soil suitability evaluation of two major crops grown in a Kupti watershed (11257 ha) located in Darwha block of Yavatmal district in Maharashtra. The study was warranted by continued persistence of the farmers to cultivate cotton and soybean crops during kharif season despite repeated failure of these crops especially cotton recently. The traditional sorghum crop that is now completely wiped out from this region was also evaluated to understand the farmers’ choice of crops. The soil information was collected through survey at 1:10000 scale in the watershed. The FAO criteria were used in conjunction with soil suitability criteria suggested by NBSS and LUP. The investigations indicated that merely 4079.0 ha area representing 36.2 % of watershed are under moderately suitable (S2), 4006.0 ha (35.6 % of TGA) under marginally suitable (S3) and 649.1 ha (5.8 % of TGA) under not suitable (N) class to grow cotton. No soils were categorized as highly suitable. While numbers for sorghum show 5096 ha area (45.3 %) under S2; 2989.0 ha (26.6 %) under S3; and 649.1 ha (5.8 %) under N class. Soybean suitability evaluation showed 5138.0 ha (45.6 %) under S2; 2947.0 ha (26.2 %) under S3 class and 649.1 ha (5.8 %) under N class. Pigeonpea suitability evaluation showed 3421 ha (30.4 %) under S1; 1966 ha (17.5 %) under S2; 2698 ha (24.0 %) under S3 and 649.1 ha (5.8 %) under N class. The results validated the traditional sorghum crop popularity in the past and gain in area grown to soybean. The farmers appear to prefer cotton despite lower soil suitability ratings due to depredation of other crops. These findings suggest that cotton is not well suited to the study area and efforts are required to promote alternate crops and/or propagate soil specific varieties adopt management practices such as mulching, life saving irrigation and broad bed furrow sowing to overcome bio-physical constraints.

The red and lateritic zone (RLZ) of Eastern India comprises of south-west part of West Bengal, a part of Odisha and Jahrkhand grimly a low position in respect of yield levels compared to many other parts of India. Soil of this area is poor in inherent fertility status with low to medium available nutrients of phosphorus, potassium and calcium. Most of the lands are located on higher elevation and erosion hazards and gully formation are very common. Monsoon rainfall is erratic or unevenly distributed in this region and, thus partial or even total failure of rainfed crops is very common. The major parts of this zone are rainfed, rice-fallow based mono-cropped and cropping intensity of the region is between 125-130%. This region of India is home of millions of resource-challenged small and marginal farmers having poor food and nutritional vis-à-vis economic security. To alleviate this grim situation of drought prone RLZ of Eastern India, intensification of Rice-fallow by inclusion of suitable crops was one the major challenges due to several bio-physical constraints. In this context, being the climate resilient and drought tolerant crops, millets may be the best choice for the rice-based crop sequence, any fallow and marginal land, agro-forestry system or even for popularizing any millet-based crop sequence for escalating the level of livelihood of the small and marginal farmers of the dry tract of RLZ. The objective is to develop a model using implementing millet-based cropping system to address food and nutritional security for the regions like RLZ of eastern India. Available research data on millet production feasibility in the region was used. A multidisciplinary approach was used for value-addition and small-scale agri-industry. In this present paper, we conceptualized the current status, prospects and research strategies for augmenting the millet production system to improve the food and nutritional security across drought prone and red laterite region of Eastern India. We believe that same model can be applied elsewhere in the world with agro-climatic conditions like RLZ.

This research presents a novel bio-inspired framework for two robots interacting together for a cooperative package delivery task with a human-in the-loop. It contributes to eliminating the need for network-based robot-robot interaction in constrained environments. An individual robot is instructed to move in specific shapes with a particular orientation at a certain speed for the other robot to infer using object detection (custom YOLOv4) and depth perception. The shape is identified by calculating the area occupied by the detected polygonal route. A metric for the area’s extent is calculated and empirically used to assign regions for specific shapes and gives an overall accuracy of 93.3% in simulations and 90% in a physical setup. Additionally, gestures are analyzed for their accuracy of intended direction, distance, and the target coordinates in the map. The system gives an average positional RMSE of 0.349 in simulation and 0.461 in a physical experiment. A video demonstration of the problem statement along with the simulations and experiments for real world applications has been given here and in Supplementary Material.

With improved characteristics and thermal stability, the nanoparticles show a significant interest in many engineering and industrial processes. The applications of nanomaterials include applications in solar energy systems, chemical reactors, heating and cooling processes and many bio-medical applications. With such motivations, this investigation aims to address the mixed convection flow of different nanoparticles due to inclined surface in presence of Newtonian heating and slip effects. Two types of nanoparticles like titanium dioxide and aluminum oxide with water base fluid are used to improve the heat transfer rate. The dimensionless governing partial differential equations for this fractional mathematical modal are accomplished by using Caputo-time fractional derivative and Fourier’s law of thermal conductivity. The generalized solution of energy and momentum equations is conquered by applying the Laplace transformation (LT) scheme. To illustrate some more physical insight of the problem and to develop the novelty of this article some special cases of velocity field are reflected whose physical clarification is well known in the literature. Furthermore, to investigate the physical implication of the under-deliberation problem, the graphical illustrations are performed by using math software MATHEMATICA. The interesting observations claimed from this investigation revealed that the interaction of thermal rate of change is more impressive for aluminium oxide. The temperature and velocity profiles are enhanced by varying the numerical value of the fractional constraint at large time scale.