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Journal articles on the topic 'Bio-ink'

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Author: Grafiati

Published: 14 March 2023

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1

Lee, Su Jeong, Jun Hee Lee, Jisun Park, Wan Doo Kim, and Su A. Park. "Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering." Materials 13, no. 16 (August 10, 2020): 3522. http://dx.doi.org/10.3390/ma13163522.

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Recently, many research groups have investigated three-dimensional (3D) bioprinting techniques for tissue engineering and regenerative medicine. The bio-ink used in 3D bioprinting is typically a combination of synthetic and natural materials. In this study, we prepared bio-ink containing porcine skin powder (PSP) to determine rheological properties, biocompatibility, and extracellular matrix (ECM) formation in cells in PSP-ink after 3D printing. PSP was extracted without cells by mechanical, enzymatic, and chemical treatments of porcine dermis tissue. Our developed PSP-containing bio-ink showed enhanced printability and biocompatibility. To identify whether the bio-ink was printable, the viscosity of bio-ink and alginate hydrogel was analyzed with different concentration of PSP. As the PSP concentration increased, viscosity also increased. To assess the biocompatibility of the PSP-containing bio-ink, cells mixed with bio-ink printed structures were measured using a live/dead assay and WST-1 assay. Nearly no dead cells were observed in the structure containing 10 mg/mL PSP-ink, indicating that the amounts of PSP-ink used were nontoxic. In conclusion, the proposed skin dermis decellularized bio-ink is a candidate for 3D bioprinting.

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2

Kim, Ji Seon, Soyoung Hong, and Changmo Hwang. "Bio-ink Materials for 3D Bio-printing." Journal of International Society for Simulation Surgery 3, no. 2 (December 10, 2016): 49–59. http://dx.doi.org/10.18204/jissis.2016.3.2.049.

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3

Jeong, Wonwoo, Min Kyeong Kim, and Hyun-Wook Kang. "Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks." Journal of Tissue Engineering 12 (January 2021): 204173142199709. http://dx.doi.org/10.1177/2041731421997091.

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Decellularized extracellular matrix-based bio-inks (dECM bio-inks) for bioprinting technology have recently gained attention owing to their excellent ability to confer tissue-specific functions and 3D-printing capability. Although decellularization has led to a major advancement in bio-ink development, the effects of detergent type, the most important factor in decellularization, are still unclear. In this study, the effects of various detergent types on bio-ink performance were investigated. Porcine liver-derived dECM bio-inks prepared using widely used detergents, including sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC), Triton X-100 (TX), and TX with ammonium hydroxide (TXA), were characterized in detail. SDS and SDC severely damaged glycosaminoglycan and elastin proteins, TX showed the lowest rate of decellularization, and TXA-based dECM bio-ink possessed the highest ECM content among all bio-inks. Differences in biochemical composition directly affected bio-ink performance, with TXA-dECM bio-ink showing the best performance with respect to gelation kinetics, intermolecular bonding, mechanical properties, and 2D/3D printability. More importantly, cytocompatibility tests using primary mouse hepatocytes also showed that the TXA-dECM bio-ink improved albumin secretion and cytochrome P450 activity by approximately 2.12- and 1.67-fold, respectively, compared with the observed values for other bio-inks. Our results indicate that the detergent type has a great influence on dECM damage and that the higher the dECM content, the better the performance of the bio-ink for 3D bioprinting.

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Han, Jonghyeuk, Wonwoo Jeong, Min-Kyeong Kim, Sang-Hyeon Nam, Eui-Kyun Park, and Hyun-Wook Kang. "Demineralized Dentin Matrix Particle-Based Bio-Ink for Patient-Specific Shaped 3D Dental Tissue Regeneration." Polymers 13, no. 8 (April 15, 2021): 1294. http://dx.doi.org/10.3390/polym13081294.

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Demineralized dentin matrix (DDM)-based materials have been actively developed and are well-known for their excellent performance in dental tissue regeneration. However, DDM-based bio-ink suitable for fabrication of engineered dental tissues that are patient-specific in terms of shape and size, has not yet been developed. In this study, we developed a DDM particle-based bio-ink (DDMp bio-ink) with enhanced three-dimensional (3D) printability. The bio-ink was prepared by mixing DDM particles and a fibrinogen–gelatin mixture homogeneously. The effects of DDMp concentration on the 3D printability of the bio-ink and dental cell compatibility were investigated. As the DDMp concentration increased, the viscosity and shear thinning behavior of the bio-ink improved gradually, which led to the improvement of the ink’s 3D printability. The higher the DDMp content, the better were the printing resolution and stacking ability of the 3D printing. The printable minimum line width of 10% w/v DDMp bio-ink was approximately 252 μm, whereas the fibrinogen–gelatin mixture was approximately 363 μm. The ink’s cytocompatibility test with dental pulp stem cells (DPSCs) exhibited greater than 95% cell viability. In addition, as the DDMp concentration increased, odontogenic differentiation of DPSCs was significantly enhanced. Finally, we demonstrated that cellular constructs with 3D patient-specific shapes and clinically relevant sizes could be fabricated through co-printing of polycaprolactone and DPSC-laden DDMp bio-ink.

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5

Lee, Su Jeong, Ji Min Seok, Jun Hee Lee, Jaejong Lee, Wan Doo Kim, and Su A. Park. "Three-Dimensional Printable Hydrogel Using a Hyaluronic Acid/Sodium Alginate Bio-Ink." Polymers 13, no. 5 (March 5, 2021): 794. http://dx.doi.org/10.3390/polym13050794.

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Bio-ink properties have been extensively studied for use in the three-dimensional (3D) bio-printing process for tissue engineering applications. In this study, we developed a method to synthesize bio-ink using hyaluronic acid (HA) and sodium alginate (SA) without employing the chemical crosslinking agents of HA to 30% (w/v). Furthermore, we evaluated the properties of the obtained bio-inks to gauge their suitability in bio-printing, primarily focusing on their viscosity, printability, and shrinkage properties. Furthermore, the bio-ink encapsulating the cells (NIH3T3 fibroblast cell line) was characterized using a live/dead assay and WST-1 to assess the biocompatibility. It was inferred from the results that the blended hydrogel was successfully printed for all groups with viscosities of 883 Pa∙s (HA, 0% w/v), 1211 Pa∙s (HA, 10% w/v), and 1525 Pa∙s, (HA, 30% w/v) at a 0.1 s−1 shear rate. Their structures exhibited no significant shrinkage after CaCl2 crosslinking and maintained their integrity during the culture periods. The relative proliferation rate of the encapsulated cells in the HA/SA blended bio-ink was 70% higher than the SA-only bio-ink after the fourth day. These results suggest that the 3D printable HA/SA hydrogel could be used as the bio-ink for tissue engineering applications.

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6

Sultan, Md Tipu, Ok Joo Lee, Joong Seob Lee, and Chan Hum Park. "Three-Dimensional Digital Light-Processing Bioprinting Using Silk Fibroin-Based Bio-Ink: Recent Advancements in Biomedical Applications." Biomedicines 10, no. 12 (December 12, 2022): 3224. http://dx.doi.org/10.3390/biomedicines10123224.

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Three-dimensional (3D) bioprinting has been developed as a viable method for fabricating functional tissues and organs by precisely spatially arranging biomaterials, cells, and biochemical components in a layer-by-layer fashion. Among the various bioprinting strategies, digital light-processing (DLP) printing has gained enormous attention due to its applications in tissue engineering and biomedical fields. It allows for high spatial resolution and the rapid printing of complex structures. Although bio-ink is a critical aspect of 3D bioprinting, only a few bio-inks have been used for DLP bioprinting in contrast to the number of bio-inks employed for other bioprinters. Recently, silk fibroin (SF), as a natural bio-ink material used for DLP 3D bioprinting, has gained extensive attention with respect to biomedical applications due to its biocompatibility and mechanical properties. This review introduces DLP-based 3D bioprinting, its related technology, and the fabrication process of silk fibroin-based bio-ink. Then, we summarize the applications of DLP 3D bioprinting based on SF-based bio-ink in the tissue engineering and biomedical fields. We also discuss the current limitations and future perspectives of DLP 3D bioprinting using SF-based bio-ink.

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7

Hsieh, Yi-Chieh, Han-Yi Wang, Kuang-Chih Tso, Chung-Kai Chang, Chi-Shih Chen, Yu-Ting Cheng, and Pu-Wei Wu. "Development of IrO2 bio-ink for ink-jet printing application." Ceramics International 45, no. 13 (September 2019): 16645–50. http://dx.doi.org/10.1016/j.ceramint.2019.05.206.

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8

Neufurth, Meik, Shunfeng Wang, Heinz C. Schröder, Bilal Al-Nawas, Xiaohong Wang, and Werner E. G. Müller. "3D bioprinting of tissue units with mesenchymal stem cells, retaining their proliferative and differentiating potential, in polyphosphate-containing bio-ink." Biofabrication 14, no. 1 (December 31, 2021): 015016. http://dx.doi.org/10.1088/1758-5090/ac3f29.

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Abstract The three-dimensional (3D)-printing processes reach increasing recognition as important fabrication techniques to meet the growing demands in tissue engineering. However, it is imperative to fabricate 3D tissue units, which contain cells that have the property to be regeneratively active. In most bio-inks, a metabolic energy-providing component is missing. Here a formulation of a bio-ink is described, which is enriched with polyphosphate (polyP), a metabolic energy providing physiological polymer. The bio-ink composed of a scaffold (N,O-carboxymethyl chitosan), a hydrogel (alginate) and a cell adhesion matrix (gelatin) as well as polyP substantially increases the viability and the migration propensity of mesenchymal stem cells (MSC). In addition, this ink stimulates not only the growth but also the differentiation of MSC to mineral depositing osteoblasts. Furthermore, the growth/aggregate pattern of MSC changes from isolated cells to globular spheres, if embedded in the polyP bio-ink. The morphogenetic activity of the MSC exposed to polyP in the bio-ink is corroborated by qRT-PCR data, which show a strong induction of the steady-state-expression of alkaline phosphatase, connected with a distinct increase in the expression ratio between RUNX2 and Sox2. We propose that polyP should become an essential component in bio-inks for the printing of cells that retain their regenerative activity.

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9

Yang, Wei, Anqianyi Tu, Yuchen Ma, Zhanming Li, Jie Xu, Min Lin, Kailong Zhang, et al. "Chitosan and Whey Protein Bio-Inks for 3D and 4D Printing Applications with Particular Focus on Food Industry." Molecules 27, no. 1 (December 28, 2021): 173. http://dx.doi.org/10.3390/molecules27010173.

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The application of chitosan (CS) and whey protein (WP) alone or in combination in 3D/4D printing has been well considered in previous studies. Although several excellent reviews on additive manufacturing discussed the properties and biomedical applications of CS and WP, there is a lack of a systemic review about CS and WP bio-inks for 3D/4D printing applications. Easily modified bio-ink with optimal printability is a key for additive manufacturing. CS, WP, and WP–CS complex hydrogel possess great potential in making bio-ink that can be broadly used for future 3D/4D printing, because CS is a functional polysaccharide with good biodegradability, biocompatibility, non-immunogenicity, and non-carcinogenicity, while CS–WP complex hydrogel has better printability and drug-delivery effectivity than WP hydrogel. The review summarizes the current advances of bio-ink preparation employing CS and/or WP to satisfy the requirements of 3D/4D printing and post-treatment of materials. The applications of CS/WP bio-ink mainly focus on 3D food printing with a few applications in cosmetics. The review also highlights the trends of CS/WP bio-inks as potential candidates in 4D printing. Some promising strategies for developing novel bio-inks based on CS and/or WP are introduced, aiming to provide new insights into the value-added development and commercial CS and WP utilization.

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10

Habib, Md Ahasan, and Bashir Khoda. "Rheological analysis of bio-ink for 3D bio-printing processes." Journal of Manufacturing Processes 76 (April 2022): 708–18. http://dx.doi.org/10.1016/j.jmapro.2022.02.048.

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11

Weng, Bo, Aoife Morrin, Roderick Shepherd, Karl Crowley, Anthony J. Killard, Peter C. Innis, and Gordon G. Wallace. "Wholly printed polypyrrole nanoparticle-based biosensors on flexible substrate." J. Mater. Chem. B 2, no. 7 (2014): 793–99. http://dx.doi.org/10.1039/c3tb21378a.

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The development of inkjet printable polypyrrole(PPy)/enzyme bio-ink successfully introduce bio-selectivity of specific bio-moleculars into conducting polymers. This method is suitable for massive industrial biosensor production.

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12

Ganpisetti, Ramesh, and Aikaterini Lalatsa. "Cellulose Bio–ink on 3D Printing Applications." Journal of Young Pharmacists 13, no. 1 (March 15, 2021): 1–6. http://dx.doi.org/10.5530/jyp.2021.13.1.

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13

Mathews, Anu Stella, Sinoj Abraham, Surjith Kumar Kumaran, Jiaxin Fan, and Carlo Montemagno. "Bio nano ink for 4D printing membrane proteins." RSC Advances 7, no. 66 (2017): 41429–34. http://dx.doi.org/10.1039/c7ra07650a.

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14

Habib, Md Ahasan, and Bashir Khoda. "Development of clay based novel bio-ink for 3D bio-printing process." Procedia Manufacturing 26 (2018): 846–56. http://dx.doi.org/10.1016/j.promfg.2018.07.105.

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15

Zhu, Jinchang, Yi He, Linlin Kong, Zhijian He, Kaylen Y. Kang, Shannon P. Grady, Leander Q. Nguyen, et al. "Digital Assembly of Spherical Viscoelastic Bio‐Ink Particles." Advanced Functional Materials 32, no. 6 (October 5, 2021): 2109004. http://dx.doi.org/10.1002/adfm.202109004.

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16

Huber, Tim, Hossein Najaf Zadeh, Sean Feast, Thea Roughan, and Conan Fee. "3D Printing of Gelled and Cross-Linked Cellulose Solutions; an Exploration of Printing Parameters and Gel Behaviour." Bioengineering 7, no. 2 (March 27, 2020): 30. http://dx.doi.org/10.3390/bioengineering7020030.

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In recent years, 3D printing has enabled the fabrication of complex designs, with low-cost customization and an ever-increasing range of materials. Yet, these abilities have also created an enormous challenge in optimizing a large number of process parameters, especially in the 3D printing of swellable, non-toxic, biocompatible and biodegradable materials, so-called bio-ink materials. In this work, a cellulose gel, made out of aqueous solutions of cellulose, sodium hydroxide and urea, was used to demonstrate the formation of a shear thinning bio-ink material necessary for an extrusion-based 3D printing. After analysing the shear thinning behaviour of the cellulose gel by rheometry a Design of Experiments (DoE) was applied to optimize the 3D bioprinter settings for printing the cellulose gel. The optimum print settings were then used to print a human ear shape, without a need for support material. The results clearly indicate that the found settings allow the printing of more complex parts with high-fidelity. This confirms the capability of the applied method to 3D print a newly developed bio-ink material.

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17

Rasyida, Amaliya, Thalyta Rizkha Pradipta, Sigit Tri Wicaksono, Vania Mitha Pratiwi, and Yeny Widya Rakhmawati. "Preliminary Study of Alginates Extracted from Brown Algae (Sargassum sp.) Available in Madura Island as Composite Based Hydrogel Materials." Materials Science Forum 964 (July 2019): 240–45. http://dx.doi.org/10.4028/www.scientific.net/msf.964.240.

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Utilization of brown algae especially in Madura, where it’s close to Surabaya, only limited for food. This become a reference for developing and increasing the potential of this algae by extracting one of the ingredients, namely alginate. This paper deals with the characterization of sodium alginate extracted from sargassum sp. using modified-purified calcium routes. The extracted sodium alginate will be further used as composite hydrogel materials and compared with commercial sodium alginate. Hereafter, the synthesized composite is expected to be bio-ink for 3d printer. Chemical composition analysis were analyzed using X-Ray Fluorosense (XRF) followed by Fourier-transform infrared spectroscopy (FTIR) analysis to identify the functional group of composite and X-Ray Diffraction (XRD). Furthermore, viscosity bath is performed to compare the viscosity of extracted and commercial one. The result shows that modified-purified calcium routes in the extraction process of sodium alginate is desirable for improving their properties. Interestingly enough, with the goal of using it as bio-ink in 3d printed fabrication, the synthesized composite shows viscosity, 300 cSt, which meets the criteria for bio-ink in 3d printer.

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18

Hu, Xueyan, Yuan Man, Wenfang Li, Liying Li, Jie Xu, Roxanne Parungao, Yiwei Wang, et al. "3D Bio-Printing of CS/Gel/HA/Gr Hybrid Osteochondral Scaffolds." Polymers 11, no. 10 (September 30, 2019): 1601. http://dx.doi.org/10.3390/polym11101601.

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Cartilage is an important tissue contributing to the structure and function of support and protection in the human body. There are many challenges for tissue cartilage repair. However, 3D bio-printing of osteochondral scaffolds provides a promising solution. This study involved preparing bio-inks with different proportions of chitosan (Cs), Gelatin (Gel), and Hyaluronic acid (HA). The rheological properties of each bio-ink was used to identify the optimal bio-ink for printing. To improve the mechanical properties of the bio-scaffold, Graphene (GR) with a mass ratio of 0.024, 0.06, and 0.1% was doped in the bio-ink. Bio-scaffolds were prepared using 3D printing technology. The mechanical strength, water absorption rate, porosity, and degradation rate of the bio-scaffolds were compared to select the most suitable scaffold to support the proliferation and differentiation of cells. P3 Bone mesenchymal stem cells (BMSCs) were inoculated onto the bio-scaffolds to study the biocompatibility of the scaffolds. The results of SEM showed that the Cs/Gel/HA scaffolds with a GR content of 0, 0.024, 0.06, and 0.1% had a good three-dimensional porous structure and interpenetrating pores, and a porosity of more than 80%. GR was evenly distributed on the scaffold as observed by energy spectrum analyzer and polarizing microscope. With increasing GR content, the mechanical strength of the scaffold was enhanced, and pore walls became thicker and smoother. BMSCs were inoculated on the different scaffolds. The cells distributed and extended well on Cs/Gel/HA/GR scaffolds. Compared to traditional methods in tissue-engineering, this technique displays important advantages in simulating natural cartilage with the ability to finely control the mechanical and chemical properties of the scaffold to support cell distribution and proliferation for tissue repair.

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19

Pan, Hui, Bolin Zheng, Hongdou Shen, Meiyuan Qi, Yinghui Shang, Chu Wu, Rongrong Zhu, Liming Cheng, and Qigang Wang. "Strength-tunable printing of xanthan gum hydrogel via enzymatic polymerization and amide bioconjugation." Chemical Communications 56, no. 23 (2020): 3457–60. http://dx.doi.org/10.1039/d0cc00326c.

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20

Lee, Dong-Hoon, Hee-Sang Cho, Dawoon Han, Rohit Chand, Tae-Jong Yoon, and Yong-Sang Kim. "Highly selective organic transistor biosensor with inkjet printed graphene oxide support system." Journal of Materials Chemistry B 5, no. 19 (2017): 3580–85. http://dx.doi.org/10.1039/c6tb03357a.

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21

Gong, Youping, Zhikai Bi, Xiangjuan Bian, Anlei Ge, Jingyang He, Wenxin Li, Huifeng Shao, Guojin Chen, and Xiang Zhang. "Study on linear bio-structure print process based on alginate bio-ink in 3D bio-fabrication." Bio-Design and Manufacturing 3, no. 2 (March 10, 2020): 109–21. http://dx.doi.org/10.1007/s42242-020-00065-9.

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22

Habib, Ahasan, and Bashir Khoda. "Development of clay based novel hybrid bio-ink for 3D bio-printing process." Journal of Manufacturing Processes 38 (February 2019): 76–87. http://dx.doi.org/10.1016/j.jmapro.2018.12.034.

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23

Taneja, Himanshu, Sandeep M. Salodkar, Avanish Singh Parmar, and Shilpi Chaudhary. "Hydrogel based 3D printing: Bio ink for tissue engineering." Journal of Molecular Liquids 367 (December 2022): 120390. http://dx.doi.org/10.1016/j.molliq.2022.120390.

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24

Ferris, Cameron J., Kerry J. Gilmore, Stephen Beirne, Donald McCallum, Gordon G. Wallace, and Marc in het Panhuis. "Bio-ink for on-demand printing of living cells." Biomater. Sci. 1, no. 2 (2013): 224–30. http://dx.doi.org/10.1039/c2bm00114d.

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25

Arguchinskaya, N. V., E. E. Beketov, E. V. Isaeva, N. S. Sergeeva, P. V. Shegay, S. A. Ivanov, and A. D. Kaprin. "Materials for creating tissue-engineered constructs using 3D bioprinting: cartilaginous and soft tissue restoration." Russian Journal of Transplantology and Artificial Organs 23, no. 1 (April 10, 2021): 60–74. http://dx.doi.org/10.15825/1995-1191-2021-1-60-74.

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3D Bioprinting is a dynamically developing technology for tissue engineering and regenerative medicine. The main advantage of this technique is its ability to reproduce a given scaffold geometry and structure both in terms of the shape of the tissue-engineered construct and the distribution of its components. The key factor in bioprinting is bio ink, a cell-laden biocompatible material that mimics extracellular matrix. To meet all the requirements, the bio ink must include not only the main material, but also other components ensuring cell proliferation, differentiation and scaffold performance as a whole. The purpose of this review is to describe the most common materials applicable in bioprinting, consider their properties, prospects and limitations in cartilage restoration.

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26

Fatimi, Ahmed, Oseweuba Valentine Okoro, Daria Podstawczyk, Julia Siminska-Stanny, and Amin Shavandi. "Natural Hydrogel-Based Bio-Inks for 3D Bioprinting in Tissue Engineering: A Review." Gels 8, no. 3 (March 14, 2022): 179. http://dx.doi.org/10.3390/gels8030179.

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Three-dimensional (3D) printing is well acknowledged to constitute an important technology in tissue engineering, largely due to the increasing global demand for organ replacement and tissue regeneration. In 3D bioprinting, which is a step ahead of 3D biomaterial printing, the ink employed is impregnated with cells, without compromising ink printability. This allows for immediate scaffold cellularization and generation of complex structures. The use of cell-laden inks or bio-inks provides the opportunity for enhanced cell differentiation for organ fabrication and regeneration. Recognizing the importance of such bio-inks, the current study comprehensively explores the state of the art of the utilization of bio-inks based on natural polymers (biopolymers), such as cellulose, agarose, alginate, decellularized matrix, in 3D bioprinting. Discussions regarding progress in bioprinting, techniques and approaches employed in the bioprinting of natural polymers, and limitations and prospects concerning future trends in human-scale tissue and organ fabrication are also presented.

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27

Maturi, Mirko, Carolina Pulignani, Erica Locatelli, Veronica Vetri Buratti, Silvia Tortorella, Letizia Sambri, and Mauro Comes Franchini. "Phosphorescent bio-based resin for digital light processing (DLP) 3D-printing." Green Chemistry 22, no. 18 (2020): 6212–24. http://dx.doi.org/10.1039/d0gc01983f.

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This work presents a novel bio-based resin for DLP 3D printing using a photocurable polyester obtained from renewable resources. The ink is formulated with phosphorescent Ir-complexes and printed for both rigid and flexible structures.

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Samimi Gharaie, Sadaf, Amir Seyfoori, Bardia Khun Jush, Xiong Zhou, Erik Pagan, Brent Godau, and Mohsen Akbari. "Silicate-Based Electro-Conductive Inks for Printing Soft Electronics and Tissue Engineering." Gels 7, no. 4 (November 27, 2021): 240. http://dx.doi.org/10.3390/gels7040240.

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Hydrogel-based bio-inks have been extensively used for developing three-dimensional (3D) printed biomaterials for biomedical applications. However, poor mechanical performance and the inability to conduct electricity limit their application as wearable sensors. In this work, we formulate a novel, 3D printable electro-conductive hydrogel consisting of silicate nanosheets (Laponite), graphene oxide, and alginate. The result generated a stretchable, soft, but durable electro-conductive material suitable for utilization as a novel electro-conductive bio-ink for the extrusion printing of different biomedical platforms, including flexible electronics, tissue engineering, and drug delivery. A series of tensile tests were performed on the material, indicating excellent stability under significant stretching and bending without any conductive or mechanical failures. Rheological characterization revealed that the addition of Laponite enhanced the hydrogel’s mechanical properties, including stiffness, shear-thinning, and stretchability. We also illustrate the reproducibility and flexibility of our fabrication process by extrusion printing various patterns with different fiber diameters. Developing an electro-conductive bio-ink with favorable mechanical and electrical properties offers a new platform for advanced tissue engineering.

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29

Baniasadi, Hossein, Rubina Ajdary, Jon Trifol, Orlando J. Rojas, and Jukka Seppälä. "Direct ink writing of aloe vera/cellulose nanofibrils bio-hydrogels." Carbohydrate Polymers 266 (August 2021): 118114. http://dx.doi.org/10.1016/j.carbpol.2021.118114.

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30

Chung, Johnson H. Y., Sina Naficy, Zhilian Yue, Robert Kapsa, Anita Quigley, Simon E. Moulton, and Gordon G. Wallace. "Bio-ink properties and printability for extrusion printing living cells." Biomaterials Science 1, no. 7 (2013): 763. http://dx.doi.org/10.1039/c3bm00012e.

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31

Hassan, S., Mohd Sallehuddin Yusof, M. I. Maksud, M. N. Nodin, and Noor Azlina Rejab. "A Feasibility Study of Roll to Roll Printing on Graphene." Applied Mechanics and Materials 799-800 (October 2015): 402–6. http://dx.doi.org/10.4028/www.scientific.net/amm.799-800.402.

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Roll to roll process is one of the famous printing techniques that are possible to create graphic and electronic device on variable substrate by using conductive ink. Graphene is an example of material that can be used as printing ink which usually used in producing micro-scale electronic devices. Here, it is proposed that extending roll to roll printing technique into the multiple micro-scale printing fine solid line onto substrate by using graphene as a printing ink. Flexography is a high speed roll to roll printing technique commonly used in paper printing industry. And this study elaborates the feasibility of graphene as a printing ink use in combination of flexography and micro-contact or micro-flexo printing for micro fine solid line. This paper will illustrates the review of graphene in producing multiple micro-solid lines printing capability for the application of printing electronic, graphic and bio-medical.

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32

Puertas-Bartolomé, María, Małgorzata K. Włodarczyk-Biegun, Aránzazu del Campo, Blanca Vázquez-Lasa, and Julio San Román. "3D Printing of a Reactive Hydrogel Bio-Ink Using a Static Mixing Tool." Polymers 12, no. 9 (August 31, 2020): 1986. http://dx.doi.org/10.3390/polym12091986.

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Hydrogel-based bio-inks have recently attracted more attention for 3D printing applications in tissue engineering due to their remarkable intrinsic properties, such as a cell supporting environment. However, their usually weak mechanical properties lead to poor printability and low stability of the obtained structures. To obtain good shape fidelity, current approaches based on extrusion printing use high viscosity solutions, which can compromise cell viability. This paper presents a novel bio-printing methodology based on a dual-syringe system with a static mixing tool that allows in situ crosslinking of a two-component hydrogel-based ink in the presence of living cells. The reactive hydrogel system consists of carboxymethyl chitosan (CMCh) and partially oxidized hyaluronic acid (HAox) that undergo fast self-covalent crosslinking via Schiff base formation. This new approach allows us to use low viscosity solutions since in situ gelation provides the appropriate structural integrity to maintain the printed shape. The proposed bio-ink formulation was optimized to match crosslinking kinetics with the printing process and multi-layered 3D bio-printed scaffolds were successfully obtained. Printed scaffolds showed moderate swelling, good biocompatibility with embedded cells, and were mechanically stable after 14 days of the cell culture. We envision that this straightforward, powerful, and generalizable printing approach can be used for a wide range of materials, growth factors, or cell types, to be employed for soft tissue regeneration.

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Han, Jonghyeuk, Da Sol Kim, Ho Jang, Hyung-Ryong Kim, and Hyun-Wook Kang. "Bioprinting of three-dimensional dentin–pulp complex with local differentiation of human dental pulp stem cells." Journal of Tissue Engineering 10 (January 2019): 204173141984584. http://dx.doi.org/10.1177/2041731419845849.

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Numerous approaches have been introduced to regenerate artificial dental tissues. However, conventional approaches are limited when producing a construct with three-dimensional patient-specific shapes and compositions of heterogeneous dental tissue. In this research, bioprinting technology was applied to produce a three-dimensional dentin–pulp complex with patient-specific shapes by inducing localized differentiation of human dental pulp stem cells within a single structure. A fibrin-based bio-ink was designed for bioprinting with the human dental pulp stem cells. The effects of fibrinogen concentration within the bio-ink were investigated in terms of printability, human dental pulp stem cell compatibility, and differentiation. The results show that micro-patterns with human dental pulp stem cells could be achieved with more than 88% viability. Its odontogenic differentiation was also regulated according to the fibrinogen concentration. Based on these results, a dentin–pulp complex having patient-specific shape was produced by co-printing the human dental pulp stem cell–laden bio-inks with polycaprolactone, which is a bio-thermoplastic used for producing the overall shape. After culturing with differentiation medium for 15 days, localized differentiation of human dental pulp stem cells in the outer region of the three-dimensional cellular construct was successfully achieved with localized mineralization. This result demonstrates the possibility to produce patient-specific composite tissues for tooth tissue engineering using three-dimensional bioprinting technology.

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Golcha, Utsav, A. S. Praveen, and D. L. Belgin Paul. "Direct ink writing of ceramics for bio medical applications – A Review." IOP Conference Series: Materials Science and Engineering 912 (September 12, 2020): 032041. http://dx.doi.org/10.1088/1757-899x/912/3/032041.

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Jose, Rod R., Waseem K. Raja, Ahmed M. S. Ibrahim, Pieter G. L. Koolen, Kuylhee Kim, Abdurrahman Abdurrob, Jonathan A. Kluge, Samuel J. Lin, Gillian Beamer, and David L. Kaplan. "Rapid prototyped sutureless anastomosis device from self-curing silk bio-ink." Journal of Biomedical Materials Research Part B: Applied Biomaterials 103, no. 7 (November 11, 2014): 1333–43. http://dx.doi.org/10.1002/jbm.b.33312.

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Jung, Hyunho, Kyungtaek Min, Heonsu Jeon, and Sunghwan Kim. "Physically Transient Distributed Feedback Laser Using Optically Activated Silk Bio-Ink." Advanced Optical Materials 4, no. 11 (July 29, 2016): 1738–43. http://dx.doi.org/10.1002/adom.201600369.

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Guo, Kai, Heran Wang, Shijie Li, Hui Zhang, Song Li, Huixuan Zhu, Zhenda Yang, Liming Zhang, Peng Chang, and Xiongfei Zheng. "Collagen-Based Thiol–Norbornene Photoclick Bio-Ink with Excellent Bioactivity and Printability." ACS Applied Materials & Interfaces 13, no. 6 (February 1, 2021): 7037–50. http://dx.doi.org/10.1021/acsami.0c16714.

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Mouser, Vivian H. M., Riccardo Levato, Anneloes Mensinga, Wouter J. A. Dhert, Debby Gawlitta, and Jos Malda. "Bio-ink development for three-dimensional bioprinting of hetero-cellular cartilage constructs." Connective Tissue Research 61, no. 2 (December 10, 2018): 137–51. http://dx.doi.org/10.1080/03008207.2018.1553960.

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Won, Joo-Yun, Mi-Hee Lee, Mi-Jeong Kim, Kyung-Hyun Min, Geunseon Ahn, Ji-Seok Han, Songwan Jin, Won-Soo Yun, and Jin-Hyung Shim. "A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink." Artificial Cells, Nanomedicine, and Biotechnology 47, no. 1 (March 15, 2019): 644–49. http://dx.doi.org/10.1080/21691401.2019.1575842.

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You, Fu, Xia Wu, Michael Kelly, and Xiongbiao Chen. "Bioprinting and in vitro characterization of alginate dialdehyde–gelatin hydrogel bio-ink." Bio-Design and Manufacturing 3, no. 1 (January 23, 2020): 48–59. http://dx.doi.org/10.1007/s42242-020-00058-8.

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El-Hennawi, H. M., A. A. Shahin, M. Rekaby, and A. A. Ragheb. "Ink jet printing of bio-treated linen, polyester fabrics and their blend." Carbohydrate Polymers 118 (March 2015): 235–41. http://dx.doi.org/10.1016/j.carbpol.2014.10.067.

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Zhang, Kun, Yanen Wang, Qinghua Wei, Xinpei Li, Ying Guo, and Shan Zhang. "Design and Fabrication of Sodium Alginate/Carboxymethyl Cellulose Sodium Blend Hydrogel for Artificial Skin." Gels 7, no. 3 (August 9, 2021): 115. http://dx.doi.org/10.3390/gels7030115.

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Tissue-engineered skin grafts have long been considered to be the most effective treatment for large skin defects. Especially with the advent of 3D printing technology, the manufacture of artificial skin scaffold with complex shape and structure is becoming more convenient. However, the matrix material used as the bio-ink for 3D printing artificial skin is still a challenge. To address this issue, sodium alginate (SA)/carboxymethyl cellulose (CMC-Na) blend hydrogel was proposed to be the bio-ink for artificial skin fabrication, and SA/CMC-Na (SC) composite hydrogels at different compositions were investigated in terms of morphology, thermal properties, mechanical properties, and biological properties, so as to screen out the optimal composition ratio of SC for 3D printing artificial skin. Moreover, the designed SC composite hydrogel skin membranes were used for rabbit wound defeat repairing to evaluate the repair effect. Results show that SC4:1 blend hydrogel possesses the best mechanical properties, good moisturizing ability, proper degradation rate, and good biocompatibility, which is most suitable for 3D printing artificial skin. This research provides a process guidance for the design and fabrication of SA/CMC-Na composite artificial skin.

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Achala Jaglan and YaminiJhanji Dhir. "Tissue Engineering – The Current Scenario & Innovations." International Journal for Modern Trends in Science and Technology 06, no. 9S (October 12, 2020): 54–57. http://dx.doi.org/10.46501/ijmtst0609s08.

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Tissue engineering is an emerging field in medical arena which combines the knowledge of science and engineering to accomplish the increasing demands to aid the damaged tissues or even a whole organ. With time various methods of tissue engineering such as traditional scaffold method ,advanced 3D bioprinting technology and the use of bio ink (the extracellular matrix materials) have become popular at various medical levels. Scaffold is a 3D structure which results in tissue formation by providing space for cells to attach, to proliferate in various directions & by secreting extracellular matrix. Also, the recent development is the use of decellularised extracellular material i.e. dECM as bio-ink to generate vascular organs like Kidney & Heart. Textiles have been playing an indispensable role in tissue engineering as it provide superior methods over other ways to fabricate scaffold. The use of smart biomaterial based scaffolds costs less and is more effective which gives advantage to tailor the tissues according to individual's tissue structure . This paper reviews the application of textiles technology in tissue engineering, various approaches of tissue engineering from traditional to the currently used approach , recent advances and its indications.

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Su, Chunyu, Yutong Chen, Shujing Tian, Chunxiu Lu, and Qizhuang Lv. "Natural Materials for 3D Printing and Their Applications." Gels 8, no. 11 (November 17, 2022): 748. http://dx.doi.org/10.3390/gels8110748.

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In recent years, 3D printing has gradually become a well-known new topic and a research hotspot. At the same time, the advent of 3D printing is inseparable from the preparation of bio-ink. Natural materials have the advantages of low toxicity or even non-toxicity, there being abundant raw materials, easy processing and modification, excellent mechanical properties, good biocompatibility, and high cell activity, making them very suitable for the preparation of bio-ink. With the help of 3D printing technology, the prepared materials and scaffolds can be widely used in tissue engineering and other fields. Firstly, we introduce the natural materials and their properties for 3D printing and summarize the physical and chemical properties of these natural materials and their applications in tissue engineering after modification. Secondly, we discuss the modification methods used for 3D printing materials, including physical, chemical, and protein self-assembly methods. We also discuss the method of 3D printing. Then, we summarize the application of natural materials for 3D printing in tissue engineering, skin tissue, cartilage tissue, bone tissue, and vascular tissue. Finally, we also express some views on the research and application of these natural materials.

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Jaywant, Swapna A., and Khalid Mahmood Arif. "Study of parameters affecting microcontact printing of thiols on gold-coated substrate." International Journal of Modern Physics B 34, no. 01n03 (November 12, 2019): 2040040. http://dx.doi.org/10.1142/s0217979220400408.

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Microcontact printing ([Formula: see text]CP) is a type of soft-lithography technique, which is widely used for patterning self-assembled monolayers (SAMs). It is a convenient method to form SAMs of bio/chemical ink onto different surfaces such as polymers, palladium, silver and gold. A wide range of applications of this technology includes micromachining, patterning proteins, cells or DNA in biosensors. However, the application primarily depends on the type of the ink used. Here, we present an experimental study that provides information about the parameters that affect the [Formula: see text]CP process. Two different thiol inks (dithiothreitol (DTT) and glutathione (GSH)) have been used for obtaining SAMs on gold-coated substrates. Our findings suggest that transferring the alkanethiols over the gold surface is extremely dependent upon the molecular weight of thiol compound, concentration of the thiol solution and pH value of the buffer used. Furthermore, higher the molecular weight, concentration and pH value of the ink, lower is the time required for the process of [Formula: see text]CP.

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Stögerer, Johannes, Sonja Baumgartner, Alexander Hochwallner, and Jürgen Stampfl. "Bio-Inspired Toughening of Composites in 3D-Printing." Materials 13, no. 21 (October 22, 2020): 4714. http://dx.doi.org/10.3390/ma13214714.

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Natural materials achieve exceptional mechanical properties by relying on hierarchically structuring their internal architecture. In several marine species, layers of stiff and hard inorganic material are separated by thin compliant organic layers, giving their skeleton both stiffness and toughness. This phenomenon is fundamentally based on the periodical variation of Young’s modulus within the structure. In this study, alteration of mechanical properties is achieved through a layer-wise build-up of two different materials. A hybrid 3D-printing device combining stereolithography and inkjet printing is used for the manufacturing process. Both components used in this system, the ink for jetting and the resin for structuring by stereolithography (SLA), are acrylate-based and photo-curable. Layers of resin and ink are solidified separately using two different light sources (λ1 = 375 nm, λ2 = 455 nm). Three composite sample groups (i.e., one hybrid material, two control groups) are built. Measurements reveal an increase in fracture toughness and elongation at break of 70% and 22%, respectively, for the hybrid material compared to the control groups. Moreover, the comparison of the two control groups shows that the effect is essentially dependent on different materials being well contained within separated layers. This bio-inspired building approach increases fracture toughness of an inherently brittle matrix material.

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Shaked, H., I. Polishchuk, A. Nagel, Y. Bekenstein, and B. Pokroy. "Long-term stabilized amorphous calcium carbonate—an ink for bio-inspired 3D printing." Materials Today Bio 11 (June 2021): 100120. http://dx.doi.org/10.1016/j.mtbio.2021.100120.

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Zhu, Jinchang, Yi He, Linlin Kong, Zhijian He, Kaylen Y. Kang, Shannon P. Grady, Leander Q. Nguyen, et al. "Digital Assembly of Spherical Viscoelastic Bio‐Ink Particles (Adv. Funct. Mater. 6/2022)." Advanced Functional Materials 32, no. 6 (February 2022): 2270036. http://dx.doi.org/10.1002/adfm.202270036.

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Kim, Min Kyeong, Wonwoo Jeong, Sang Min Lee, Jeong Beom Kim, Songwan Jin, and Hyun-Wook Kang. "Decellularized extracellular matrix-based bio-ink with enhanced 3D printability and mechanical properties." Biofabrication 12, no. 2 (January 31, 2020): 025003. http://dx.doi.org/10.1088/1758-5090/ab5d80.

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Salehi, Mohammad Mahdi, and Maryam Ataeefard. "Micro powder poly lactic acid/carbon black composite as a bio printing ink." Journal of Composite Materials 53, no. 17 (February 12, 2019): 2407–14. http://dx.doi.org/10.1177/0021998319828154.

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