Relevant bibliographies by topics / Bio-conjugation

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Bio-conjugation'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 March 2023

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Journal articles on the topic "Bio-conjugation"

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Jeon, Minjeong, Suhyun Jung, and Seongsoon Park. "Facile covalent bio-conjugation of hydroxyapatite." New Journal of Chemistry 42, no. 18 (2018): 14870–75. http://dx.doi.org/10.1039/c8nj02766h.

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Ang, Wei Li, Jiri Sturala, Nikolas Antonatos, Zdeněk Sofer, and Alessandra Bonanni. "Effect of surface chemistry on bio-conjugation and bio-recognition abilities of 2D germanene materials." Nanoscale 13, no. 3 (2021): 1893–903. http://dx.doi.org/10.1039/d0nr07579e.

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The surface ligands on chemically modified germanenes have strong influence on the intrinsic fluorescence, on the bio-conjugation ability and the bio-recognition efficiency of the material towards the detection of a specific analyte.
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Shao, Fangwei, Ralph Weissleder, and Scott A. Hilderbrand. "Monofunctional Carbocyanine Dyes for Bio- and Bioorthogonal Conjugation." Bioconjugate Chemistry 19, no. 12 (December 17, 2008): 2487–91. http://dx.doi.org/10.1021/bc800417b.

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Torchynska, Tetyana V., Yuri V. Vorobiev, and Paul P. Horley. "Modeling of the effect of bio-conjugation to anti-interleukin-10 antibodieson the photoluminescence of CdSe/ZnS quantum dots." MRS Proceedings 1617 (2013): 145–50. http://dx.doi.org/10.1557/opl.2013.1177.

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ABSTRACTBio-conjugated CdSe/ZnS core/shell quantum dots (QDs) attract essential scientific interest due to their possible nano-medicine applications, including selective highlighting of affected tissues and targeted drug delivery to the certain type of cells. The paper is focused on the theoretical description of the blue shift observed in the luminescence spectra of CdSe/ZnS QDs upon their bio-conjugation with the anti-interleukin-10 antibodies. We propose a model that describes the ground state of the exciton confined in a quantum dot and explaining the bio-conjugation phenomenon by the change of the effective confinement volume.
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Jia, Fei, Shuyu Lv, and Sha Xu. "Correction: Bio-conjugation of graphene quantum dots for targeting imaging." RSC Advances 9, no. 53 (2019): 30888–89. http://dx.doi.org/10.1039/c9ra90067e.

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Oteng-Pabi, Samuel K., Christophe Pardin, Maria Stoica, and Jeffrey W. Keillor. "Site-specific protein labelling and immobilization mediated by microbial transglutaminase." Chem. Commun. 50, no. 50 (2014): 6604–6. http://dx.doi.org/10.1039/c4cc00994k.

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Jia, Fei, Shuyu Lv, and Sha Xu. "Bio-conjugation of graphene quantum dots for targeting imaging." RSC Advances 7, no. 84 (2017): 53532–36. http://dx.doi.org/10.1039/c7ra11963a.

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We demonstrate GQD-based bio-conjugation. Targeted imaging can be achieved in both cells and tissue models with single or multi-color staining, showing universality for different kinds of biological models.
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Huang, Xin, Hidekazu Ishitobi, and Yasushi Inouye. "Formation of fluorescent platinum nanoclusters using hyper-branched polyethylenimine and their conjugation to antibodies for bio-imaging." RSC Advances 6, no. 12 (2016): 9709–16. http://dx.doi.org/10.1039/c5ra24522b.

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Borkovska, L., N. Korsunska, T. Stara, O. Kolomys, V. Strelchuk, T. Kryshtab, S. Ostapenko, G. Chornokur, and C. M. Phelan. "Micro-Photoluminescence Study of Bio-conjugated CdSe/ZnS Nanocrystals." MRS Proceedings 1617 (2013): 157–62. http://dx.doi.org/10.1557/opl.2013.1179.

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ABSTRACTThe effect of bio-conjugation of CdSe/ZnS core-shell quantum dots (QDs) with Interleukin 10 (IL-10) antibodies on the aging of photoluminescence (PL) spectra of the QDs was investigated. The aging occurred upon storage of QDs for about 2 years or thermal annealing at 190 oC for up to 12 hours at atmospheric ambience and consisted in “blue” shifting the PL band position, increasing a PL band half-width and decreasing the PL intensity. The bio-conjugation is found to promote PL aging. The aging upon storage is attributed to the oxidation that decreases the QD core dimension, while the aging upon thermal annealing can be due to both oxidation and alloying of CdSe core and ZnS shell.
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Palanna, Manjunatha, Imadadulla Mohammed, Shambhulinga Aralekallu, Manjunatha Nemakal, and Lokesh Koodlur Sannegowda. "Simultaneous detection of paracetamol and 4-aminophenol at nanomolar levels using biocompatible cysteine-substituted phthalocyanine." New Journal of Chemistry 44, no. 4 (2020): 1294–306. http://dx.doi.org/10.1039/c9nj05252f.

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Dissertations / Theses on the topic "Bio-conjugation"

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Oteng-Pabi, Samuel. "Identification and Characterization of Peptide Substrates of Bacterial Transglutaminases for Use in Bio-conjugation and Bio-catalytic Applications." Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/36184.

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Transglutaminases (protein-glutamine:amine y-glutamyl- transferase, EC 2.3.2.13) are a family of calcium-dependent enzymes which catalyze an acyl transfer between glutamine residues and a wide variety of primary amines. When lysine acts as the acyl-acceptor substrate, α-glutamyl lysine isopeptide bond is formed. Isopeptide catalyzation results in protein cross-linkage which is prevalent throughout biological processes. Microbial transglutaminase (mTG) is a bacterial variant of the transglutaminase family, distinct by virtue of its calcium-independent catalysis of the isopeptidic bond. Furthermore, mTGs promiscuity in donor substrate preference highlights its biocatalytic potential. To realize the potential of the enzyme, a high-reactivity tag was necessary for protein labelling. To address this, an enzyme-coupled assay was developed to characterize peptides in the hopes of developing orthogonal substrates to facilitate mTG-mediated labelling and biocatalysis. The discovery of high-reactivity peptide tags allowed the realization of in vitro protein labelling- facilitated by mTG. The 7M48 peptide was fused to a test protein, where it was subsequently propargylated with propargyl amine to fluorescently label or immobilize a test protein. Although there are endless possibilities for in vitro bio-conjugation through mTG, proteolytic activation limits any in-cell labelling strategies with this enzyme. To circumvent this issue, development of an alternative bacterial enzyme, Bacillus subtilis transglutaminase (bTG), was chosen to replace mTG. bTG maintains the advantages associated with mTG but is expressed in its active form. Unlike mTG, there is limited preliminary research associated with the enzyme or its substrate scope. To better understanding substrate reactivity, a FRET-based assay was developed allows for the discovery of new high-reactivity peptides for bTG. These peptides were then used in labelling strategies to demonstrate the potential bTG-mediated bioconjugation. This strategy includes the added advantage of potential for in-cellulo labelling.
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Preuß, Corinna Marina [Verfasser], and C. [Akademischer Betreuer] Barner-Kowollik. "Fusing Bio-Inspired Surface Modification with Cycloaddition-Driven Polymer Conjugation / Corinna Marina Preuß. Betreuer: C. Barner-Kowollik." Karlsruhe : KIT-Bibliothek, 2015. http://d-nb.info/1070584371/34.

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COCHET, FLORENT. "Design, synthesis and bioconjugation of TLR4 ligands to understand and modulate innate immunity processes." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2019. http://hdl.handle.net/10281/241113.

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Toll-like receptors (TLRs) are among the first receptors activated during host-pathogen interactions. They act as key mediators in the pathogen-associated molecular patterns (PAMPs) detection and are responsible for the innate and adaptive immune responses in mammal. Belonging to this family, Toll-like receptor 4 (TLR4) is the mammalian sensor of bacterial endotoxin, lipopolysaccharide (LPS). Its activation results in the rapid triggering of pro-inflammatory processes essential for optimal host immune responses. TLR4 activation is a complex event which involves several proteins (LBP, CD14, and MD-2) and ends with the dimerization as an activated (TLR4/MD-2/LPS)2 complex. Dysregulated TLR4 activation is related to an impressively broad spectrum of disorders still lacking specific pharmacological treatment, including autoimmune disorders, chronic inflammations, allergies, asthma, infectious and central nervous system diseases, cancer, and sepsis. TLR4 modulation by small molecules of synthetic and natural origin provides access to new TLR-based therapeutics. Indeed, compounds able to block TLR4 activation (antagonists) are promising drug candidates against thee pathologies while TLR4 activating compounds (agonists) may be use as vaccines adjuvants and antitumoral agents. The work of this PhD thesis is described in four papers. The aim of the thesis is to study the processes and requirements leading to TLR4 activation/inhibition. The first study, Chap. IX, focuses on the contribution of carbohydrates to bacterial endotoxin (lipopolysaccharide, LPS, lipooligosaccharide, LOS, and lipid A) activity and in the switch from TLR4 agonism to antagonism. In particular, the structure-activity relationship and contribution of core saccharides 3-deoxy-D-manno-octulosonic acid (Kdo) and heptosyl-2-keto-3-deoxy-octulosonate (Hep) to TLR4/MD-2 binding and activation by LPS and LOS have been investigated in detail. This study allows the rational design of new structures of potential TLR4 modulators. These compounds were projected by computer-assisted design and their binding to MD-2 was evaluated through docking studies. A set of the most promising compounds was defined. They are composed of a glucosamine-bis-succinyl core (two carboxylate groups as phosphates bioisosteres) linked to different unsaturated and saturated fatty acid chains. The binding of the synthetic compounds to MD-2 was studied by four independent methods using functional recombinant human MD-2 protein purified. It was demonstrated that all compounds bound to MD-2 with similar affinities and inhibited, in a concentration-dependent manner, the LPS-stimulated TLR4 signaling and cytokine production in human and murine cells.
Toll-like receptors (TLRs) are among the first receptors activated during host-pathogen interactions. They act as key mediators in the pathogen-associated molecular patterns (PAMPs) detection and are responsible for the innate and adaptive immune responses in mammal. Belonging to this family, Toll-like receptor 4 (TLR4) is the mammalian sensor of bacterial endotoxin, lipopolysaccharide (LPS). Its activation results in the rapid triggering of pro-inflammatory processes essential for optimal host immune responses. TLR4 activation is a complex event which involves several proteins (LBP, CD14, and MD-2) and ends with the dimerization as an activated (TLR4/MD-2/LPS)2 complex. Dysregulated TLR4 activation is related to an impressively broad spectrum of disorders still lacking specific pharmacological treatment, including autoimmune disorders, chronic inflammations, allergies, asthma, infectious and central nervous system diseases, cancer, and sepsis. TLR4 modulation by small molecules of synthetic and natural origin provides access to new TLR-based therapeutics. Indeed, compounds able to block TLR4 activation (antagonists) are promising drug candidates against thee pathologies while TLR4 activating compounds (agonists) may be use as vaccines adjuvants and antitumoral agents. The work of this PhD thesis is described in four papers. The aim of the thesis is to study the processes and requirements leading to TLR4 activation/inhibition. The first study, Chap. IX, focuses on the contribution of carbohydrates to bacterial endotoxin (lipopolysaccharide, LPS, lipooligosaccharide, LOS, and lipid A) activity and in the switch from TLR4 agonism to antagonism. In particular, the structure-activity relationship and contribution of core saccharides 3-deoxy-D-manno-octulosonic acid (Kdo) and heptosyl-2-keto-3-deoxy-octulosonate (Hep) to TLR4/MD-2 binding and activation by LPS and LOS have been investigated in detail. This study allows the rational design of new structures of potential TLR4 modulators. These compounds were projected by computer-assisted design and their binding to MD-2 was evaluated through docking studies. A set of the most promising compounds was defined. They are composed of a glucosamine-bis-succinyl core (two carboxylate groups as phosphates bioisosteres) linked to different unsaturated and saturated fatty acid chains. The binding of the synthetic compounds to MD-2 was studied by four independent methods using functional recombinant human MD-2 protein purified. It was demonstrated that all compounds bound to MD-2 with similar affinities and inhibited, in a concentration-dependent manner, the LPS-stimulated TLR4 signaling and cytokine production in human and murine cells.
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Devulapally, Praneeth Reddy. "Bio-Conjugation of Yersinia pseudo-tuberculosis proteins YscUC and YscP in the presence of DMPG lipid membrane vesicles." Thesis, Umeå universitet, Kemiska institutionen, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-58794.

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GALBIATI, ELISABETTA. "Investigating the biological activity of proteins immobilized on colloidal nanoparticles." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2014. http://hdl.handle.net/10281/52342.

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Currently, nanoparticles (NPs) play an increasing role in biomedical research and clinical applications, thanks to their peculiar optical, physical and chemical properties. A great challenge in nanodiagnostics is the development of new nano-sized devices aimed to optimize the detection of primary cancer cells and metastases. The design of ideal nanoconjugates, containing bioactive ligands specific for targeting cancer cell, requires optimization of fundamental parameters involved in conjugation reactions: both functional conformation and proper orientation must be preserved. This characteristic determines bioactivity, avidity and targeting efficiency of the functionalized NPs. In the context of this thesis, different conjugation strategies were analyzed, focusing on the improvement of the biological activity of the immobilized protein. First of all, trastuzumab-functionalized pegylated iron oxide nanoparticles were synthetized and protein conformation analyzed using FTIR spectroscopy. This technique provides direct evidence of the extent of native structure preservation of the immobilized protein, in dependence of the conjugation strategy. Moreover, the possibility to control the ligand/peptide orientation on the nanoparticle surface is a fundamental step to optimize receptor recognition. An elegant strategy involves the use of fusion proteins containing a small enzyme (defined “capture protein”) capable of irreversibly cross-coupling with a suicide inhibitor anchored to the solid surface. Three different approaches have been analyzed: SNAP (O6-alkylguanine-DNA-transferase), HALO (haloalkane dehalogenase) and cutinase enzymes fused with specific proteins or small peptides for the selective targeting of breast cancer cells. Although targeted therapy with monoclonal antibody, or small portion of these proteins, is a major treatment currently employed in many cancers, the use of short peptides as targeting moieties of tumor receptors have several advantages. The possibility to exploit gold nanoparticles (AuNPs) properties, to form a self-assembled monolayer on AuNPs surfaces, allows to increase ligand-receptor target affinity/recognition. The capability of all these bioconjugation methods to specifically and selectively target breast cancer cells, was confirmed by flow cytometry (FACS), confocal laser scanning microscopy and transmission electron microscopy (TEM).
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GRIMALDI, Natascia. "Polymeric and bio-hybrid nanovectors for drug delivery and imaging devices." Doctoral thesis, Università degli Studi di Palermo, 2014. http://hdl.handle.net/10447/91198.

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Nanotechnology applied to the Medicine is providing new tools to the current therapeutic and diagnostic approaches to fight cancer and other diseases. However, many of the proposed nanodevices show some deficits related to both their inherent properties and performance, and the synthetic strategies proposed for their production. In the present work, a new promising approach based on e-beam radiation-induced radical crosslinking of a water soluble, biocompatible synthetic polymer has been developed. In particular, the possibility of generating Poly-N-(Vinyl- Pyrrolidone)(PVP)-based nanocarriers, i.e. nanogels with a base PVP structure, tailored physico-chemical properties (particles size distribution, surface charge density) and multifunctionality has been explored. A thorough product analysis on the generated nanoparticles through different characterization techniques, such as dynamic and static light scattering, photo-correlation spectroscopy, FT-IR, Raman, solid state NMR and XPS spectroscopies, SEM and AFM, has been carried. PVPbased nanogels have been then used as building blocks for the assembly of tumortarget “composite” nanodevices. “Model” ligands with various biological functions and drugs have been conjugated to the nanogels. Moreover, the biocompatibility and localization pattern of the nanocarriers in cell cultures have been evaluated. It has been demonstrated that all the NGs produced are biocompatible and able to be internalized by cells. Furthermore, the many functional groups grafted on the NGs are available for coupling reactions with bioactive molecules, such as targeting moieties, drugs and metal-ions chelating agents. This collective evidence validates the generated nanostructures for the intent they have been designed for, i.e. as nanocarriers in the biomedical field. E-beam irradiation using industrial type accelerators has demonstrated to be a viable manufacturing process since it grants high yields in terms of recovered product and high throughputs. Moreover, through a proper selection of the experimental parameters, this approach has allowed to obtain NGs with the desired properties, in terms of size, surface charge density, degree of crosslinking and functionality. All the evidences collected in this study, in terms of favorable properties-byprocess of the nanostructures generated and inherent advantages in the manufacturing process developed, can represent the fundaments for a further development and evaluation of this versatile “nanomaterial platform” for the treatment and diagnosis of various diseases, and cancer in particular.
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Frazzoni, Marcello. "Complessi metallici come “probes” luminescenti per avidina: design e sintesi di leganti bifunzionali piridintriazolo-biotina." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amslaurea.unibo.it/3023/.

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Studi recenti sull’utilizzo di sonde organometalliche per bioconiugazione sfruttano la possibilità di interazione di queste con una proteina, l’avidina, per la sua capacità di coordinarsi selettivamente ad una particolare molecola organica, la biotina. In questa tesi viene descritta la sintesi di due leganti bi-funzionali contenenti sia un’unità capace di fungere da legante per un opportuno metallo, che un’unità di biotina in grado di legarsi all’avidina. La differenza fra i due leganti risiede nello spaziatore che collega le due unità funzionali della molecola. Una volta ottenuti i leganti progettati, sono stati sintetizzati i corrispondenti complessi di Ir (III) e Re (I). Le proprietà fotofisiche sono state misurate sia in solvente organico che in soluzione acquosa e quindi sono state effettuate titolazioni dell’avidina con soluzioni acquose a titolo noto dei complessi sintetizzati, con il fine di comprendere come le proprietà luminescenti dei complessi vengano influenzate dalla bio-coniugazione tra biotina ed avidina.
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Dezfouli, Mahya. "Barcoded DNA Sequencing for Parallel Protein Detection." Doctoral thesis, KTH, Genteknologi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-159506.

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The work presented in this thesis describes methodologies developed for integration and accurate interpretation of barcoded DNA, to empower large-scale-omics analysis. The objectives mainly aim at enabling multiplexed proteomic measurements in high-throughput format through DNA barcoding and massive parallel sequencing. The thesis is based on four scientific papers that focus on three main criteria; (i) to prepare reagents for large-scale affinity-proteomics, (ii) to present technical advances in barcoding systems for parallel protein detection, and (iii) address challenges in complex sequencing data analysis. In the first part, bio-conjugation of antibodies is assessed at significantly downscaled reagent quantities. This allows for selection of affinity binders without restrictions to accessibility in large amounts and purity from amine-containing buffers or stabilizer materials (Paper I). This is followed by DNA barcoding of antibodies using minimal reagent quantities. The procedure additionally enables efficient purification of barcoded antibodies from free remaining DNA residues to improve sensitivity and accuracy of the subsequent measurements (Paper II). By utilizing a solid-phase approach on magnetic beads, a high-throughput set-up is ready to be facilitated by automation. Subsequently, the applicability of prepared bio-conjugates for parallel protein detection is demonstrated in different types of standard immunoassays (Papers I and II). As the second part, the method immuno-sequencing (I-Seq) is presented for DNAmediated protein detection using barcoded antibodies. I-Seq achieved the detection of clinically relevant proteins in human blood plasma by parallel DNA readout (Paper II). The methodology is further developed to track antibody-antigen interaction events on suspension bead arrays, while being encapsulated in barcoded emulsion droplets (Paper III). The method, denoted compartmentalized immuno-sequencing (cI-Seq), is potent to perform specific detections with paired antibodies and can provide information on details of joint recognition events. Recent progress in technical developments of DNA sequencing has increased the interest in large-scale studies to analyze higher number of samples in parallel. The third part of this thesis focuses on addressing challenges of large-scale sequencing analysis. Decoding of a huge DNA-barcoded data is presented, aiming at phase-defined sequence investigation of canine MHC loci in over 3000 samples (Paper IV). The analysis revealed new single nucleotide variations and a notable number of novel haplotypes for the 2nd exon of DLA DRB1. Taken together, this thesis demonstrates emerging applications of barcoded sequencing in protein and DNA detection. Improvements through the barcoding systems for assay parallelization, de-convolution of antigen-antibody interactions, sequence variant analysis, as well as large-scale data interpretation would aid biomedical studies to achieve a deeper understanding of biological processes. The future perspectives of the developed methodologies may therefore stem for advancing large-scale omics investigations, particularly in the promising field of DNA-mediated proteomics, for highly multiplex studies of numerous samples at a notably improved molecular resolution.

QC 20150203

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Vecchi, Simone <1984&gt. "Conjugation of Toll Like Receptor 7 agonist through conjugation to immunogenic proteins. Synthesis, characterization, formulation and pre-clinical evaluation." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2013. http://amsdottorato.unibo.it/5758/1/vecchi_simone_tesi.pdf.

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The next generation of vaccine adjuvant are represented by a wide ranging set of molecules called Toll like agonists (TLR’s). Although many of these molecules are complex structures extracted from microorganisms, small molecule TLR agonists have also been identified. However, delivery systems have not been optimized to allow their effective delivery in conjunction with antigens. Here we describe a novel approach in which a small molecule TLR agonist has been conjugated directly to antigens to ensure effective co delivery. We describe the conjugation of a relevant protein, a recombinant protective antigen from S.pneumoniae (RrgB), which is linked to a TLR7 agonist. Following thorough characterization to ensure there was no aggregation, the conjugate was evaluated in a murine infection model. Results showed that the conjugate extended animals’ survival after lethal challenge with S.pneumoniae. Comparable results were obtained with a 10 fold lower dose than that of the native unconjugated antigen. Notably, the animals immunized with the same dose of unconjugated TLR7 agonist and antigen showed no adjuvant effect. The increased immunogenicity was likely a consequence of the co-localization of TLR7 agonist and antigen by chemical binding and is was more effective than simple co-administration. Likely, this approach can be adopted to reduce the dose of antigen required to induce protective immunity, and potentially increase the safety of a broad variety of vaccine candidates
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Vecchi, Simone <1984&gt. "Conjugation of Toll Like Receptor 7 agonist through conjugation to immunogenic proteins. Synthesis, characterization, formulation and pre-clinical evaluation." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2013. http://amsdottorato.unibo.it/5758/.

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The next generation of vaccine adjuvant are represented by a wide ranging set of molecules called Toll like agonists (TLR’s). Although many of these molecules are complex structures extracted from microorganisms, small molecule TLR agonists have also been identified. However, delivery systems have not been optimized to allow their effective delivery in conjunction with antigens. Here we describe a novel approach in which a small molecule TLR agonist has been conjugated directly to antigens to ensure effective co delivery. We describe the conjugation of a relevant protein, a recombinant protective antigen from S.pneumoniae (RrgB), which is linked to a TLR7 agonist. Following thorough characterization to ensure there was no aggregation, the conjugate was evaluated in a murine infection model. Results showed that the conjugate extended animals’ survival after lethal challenge with S.pneumoniae. Comparable results were obtained with a 10 fold lower dose than that of the native unconjugated antigen. Notably, the animals immunized with the same dose of unconjugated TLR7 agonist and antigen showed no adjuvant effect. The increased immunogenicity was likely a consequence of the co-localization of TLR7 agonist and antigen by chemical binding and is was more effective than simple co-administration. Likely, this approach can be adopted to reduce the dose of antigen required to induce protective immunity, and potentially increase the safety of a broad variety of vaccine candidates
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Book chapters on the topic "Bio-conjugation"

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Gedda, Gangaraju, Arun Bhupathi, and V. L. N. Balaji Gupta Tiruveedhi. "Naturally Derived Carbon Dots as Bioimaging Agents." In BioMechanics and Functional Tissue Engineering [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96912.

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The recent advances in nanoscience and technology have opened new avenues for carbon-based nanomaterials. Especially, Carbon dots (CDs) have gained significant attention due to their simple, economic and rapid green synthesis. These materials exhibit excellent water solubility, fluorescence emission, high fluorescence quantum yield, Ultraviolet (UV) to Infrared (IR) range absorbance and high bio-compatibility. Therefore, these materials are widely used for various biological applications including bio-imaging. With the integration and doping of surface passive agents and elements, respectively influenced the enhancement of fluorescence property of CDs. Also, the conjugation of receptor-based targeting ligands leads to targeted bioimaging. CDs in combination with other imaging contrast agents lead to the development of novel contrast agents for bimodal imaging and multimodal imaging techniques. The combination of diagnostic CDs with therapeutic agents resulted in the formation of theragnostic CDs for image guided therapies. In this chapter, a comprehensive view on the top-down and bottom–up green synthesis methods for naturally derived CDs discussed. Further, unique physical, chemical, optical and biological properties of CDs described. Finally, fluorescence based bimodal and multimodal imaging techniques also described.
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AHOTUPA, MARKKU, and TOMMI J. VASANKARI. "BASELINE DIENE CONJUGATION IN LDL LIPIDS: AN INDICATOR OF CIRCULATING OXIDIZED LDL." In Bio-Assays for Oxidative Stress Status, 7–16. Elsevier, 2001. http://dx.doi.org/10.1016/b978-0-444-50957-4.50006-5.

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Conference papers on the topic "Bio-conjugation"

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Yang, Xi, Zewen Han, Yuan Gong, Gang-Ding Peng, and Yun-Jiang Rao. "Wash-out-free fiber optofluidic laser by sequential bio-conjugation." In Asia Communications and Photonics Conference. Washington, D.C.: OSA, 2020. http://dx.doi.org/10.1364/acpc.2020.s3i.2.

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Li, Zhenyu, Yan-Yu Chen, Sha Xiong, Nguyen Kim Truc, Qihua Xiong, and Ai-Qun Liu. "Ultracompact high-sensitivity biosensing based on regenerable laminated bio-conjugation in integrated photonic circuits." In Biosensing and Nanomedicine XI, edited by Hooman Mohseni, Massoud H. Agahi, and Manijeh Razeghi. SPIE, 2018. http://dx.doi.org/10.1117/12.2321211.

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Vennila Preethi, S., V. Geetha Gayathri, J. Jeffrey Calwin, Sharmila, Jayamani, and Sujitha. "Synthesis of silver nanoparticles from mimosa pudica and bio-conjugation with hydroxyapatite for orthopaedic application." In INTERNATIONAL CONFERENCE ON SYSTEMATIZATION, SCIENCE AND SUPERVISION: ICSSS - 2021. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0105011.

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