Relevant bibliographies by topics / Binding component

Academic literature on the topic 'Binding component'

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Published: 30 May 2022
Last updated: 31 May 2022

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Journal articles on the topic "Binding component"

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de Frutos, Pablo García, Ylva Härdig, and Björn Dahlbäck. "Serum Amyloid P Component Binding to C4b-binding Protein." Journal of Biological Chemistry 270, no. 45 (November 10, 1995): 26950–55. http://dx.doi.org/10.1074/jbc.270.45.26950.

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Comis, Alfio, and Simon B. Easterbrook-Smith. "Binding of complement component C1q by spectrin." Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 870, no. 3 (April 1986): 426–31. http://dx.doi.org/10.1016/0167-4838(86)90250-5.

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SAKAMOTO, KAZUYUKI, H. M. ZHANG, and ROGER I. G. UHRBERG. "HIGH-RESOLUTION Si2p CORE-LEVEL STUDY OF THE K/Si(111)-(3 × 1) SURFACE." Surface Review and Letters 09, no. 02 (April 2002): 1235–39. http://dx.doi.org/10.1142/s0218625x02003561.

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The structure of the K/Si (111)-(3 × 1) surface was studied by high-resolution core-level photoelectron spectroscopy. Five surface components were observed in the Si 2p core-level spectra. Compared to the bulk component, three components are shifted to lower and two to higher binding energies. The two components with the lowest binding energies are assigned to the top-layer Si atoms bonded to the K atoms with different configurations. The component with highest binding energy has a contribution from the π-bonded Si atoms of the top layer. The two other components originate from the Si atoms of the second and third layers.
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Seibold, Julia C., Sophie Nolden, Josefa Oberem, Janina Fels, and Iring Koch. "The binding of an auditory target location to a judgement: A two-component switching approach." Quarterly Journal of Experimental Psychology 72, no. 8 (February 15, 2019): 2056–67. http://dx.doi.org/10.1177/1747021819829422.

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In a two-component switching paradigm, in which participants switched between two auditory attention selection criteria (attention component: left vs. right ear) and two judgements (judgement component: number vs. letter judgement), we found high judgement switch costs in attention criterion repetitions, but low costs in attention criterion switches. This finding showed an interaction of components. Previous two-component switching studies observed differently emphasised interaction patterns. In the present study, we explored whether the strength of the interaction pattern reflects the strength of the binding of target location and judgement. Specifically, we investigated whether exogenous target location cueing led to weaker binding than endogenous cueing, and whether preparation for ear selection could influence the binding. Attention switches with auditory exogenous target location cues did not affect the component interaction pattern, whereas a prolonged preparation interval led to a more emphasised pattern. Binding between target location and judgement may therefore be rather automatic and may not necessarily require concurrent component processing. Sufficient time for target location switches with long preparation time may activate the previous trial’s episode or facilitate switches of the subsequent judgement.
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Golik, V. I., Yu I. Razorenov, V. S. Vagin, and V. I. Lyashenko. "Efficiency of mineral additives to binding component for making hardening mixtures at underground operations." Ferrous Metallurgy. Bulletin of Scientific , Technical and Economic Information 77, no. 6 (June 21, 2021): 643–50. http://dx.doi.org/10.32339/0135-5910-2021-6-643-650.

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For effective operation of mining enterprises, including mines of ferrous metallurgy, supply of quality binding components is necessary to make hardening mixtures for packing of goave, aroused at underground development of hard mineral deposits. Results of the study of compositions based on ash-cement, nepheline, belit-aluminates and lime binding component made of mining and metallurgical production wastes presented. Application of vibro-, mechanical- and electrochemical activation methods to obtain filling mixtures from local low-quality raw materials, as well as activation of binding components were analyzed. A model to evaluate efficiency of binding additives presented. It was shown that decrease of cement consumption by addition of binding components of mineral origin requires perfection of mining processes, first of all, grinding and activation. It was proved, that additions of electric filters ash, nephelines, belit-aluminates and lime, obtained by utilization of current and old tailings to the basic binding component – portland cement – makes it possible to obtain hardening filling mixtures, having high enough strength to apply them at goave packing. Application of the study results makes it perspective for depressed mining and related enterprises to survive under conditions of the forming market.
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Jessberger, Nadja, Richard Dietrich, Stefanie Schwemmer, Franziska Tausch, Valerie Schwenk, Andrea Didier, and Erwin Märtlbauer. "Binding to The Target Cell Surface Is The Crucial Step in Pore Formation of Hemolysin BL from Bacillus cereus." Toxins 11, no. 5 (May 20, 2019): 281. http://dx.doi.org/10.3390/toxins11050281.

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A major virulence factor involved in Bacillus cereus food poisoning is the three-component enterotoxin hemolysin BL. It consists of the binding component B and the two lytic components L1 and L2. Studying its mode of action has been challenging, as natural culture supernatants additionally contain Nhe, the second three-component enterotoxin, and purification of recombinant (r) Hbl components has been difficult. In this study, we report on pore-forming, cytotoxic, cell binding and hemolytic activity of recently generated rHbl components expressed in E. coli. It is known that all three Hbl components are necessary for cytotoxicity and pore formation. Here we show that an excess of rHbl B enhances, while an excess of rHbl L1 hinders, the velocity of pore formation. Most rapid pore formation was observed with ratios L2:L1:B = 1:1:10 and 10:1:10. It was further verified that Hbl activity is due to sequential binding of the components B - L1 - L2. Accordingly, all bioassays proved that binding of Hbl B to the cell surface is the crucial step for pore formation and cytotoxic activity. Binding of Hbl B took place within minutes, while apposition of the following L1 and L2 occurred immediately. Further on, applying toxin components simultaneously, it seemed that Hbl L1 enhanced binding of B to the target cell surface. Overall, these data contribute significantly to the elucidation of the mode of action of Hbl, and suggest that its mechanism of pore formation differs substantially from that of Nhe, although both enterotoxin complexes are sequentially highly related.
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Zammit, V. A., and C. G. Corstorphine. "Effects of incubation at physiological temperatures on the concentration-dependence of [2-14C]malonyl-CoA binding to rat liver mitochondria." Biochemical Journal 231, no. 2 (October 15, 1985): 343–47. http://dx.doi.org/10.1042/bj2310343.

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Specific binding of [2-14C] malonyl-CoA to rat liver mitochondria was measured at different temperatures and after various periods of time of exposure of the mitochondria to the ligand. Incubation of mitochondria at 37 degrees C in the absence of malonyl-CoA resulted in a decrease in their ability to bind malonyl-CoA at all concentrations tested (up to 55 microM). However, incubation of mitochondria in the presence of malonyl-CoA resulted in the loss of the binding only by a low-affinity component. By contrast, there was an increase in the binding that occurred at low, physiological, concentrations of malonyl-CoA. These differences in the response of the two binding components to incubation conditions were used to obtain quantitative data about their respective saturation kinetics. Evidence was obtained that, whereas the high-affinity component approached saturation hyperbolically with respect to malonyl-CoA concentration, the low-affinity component had sigmoidal characteristics. The concentrations of malonyl-CoA required to half-saturate the two components were 2-3 microM and 30 microM for the high- and low-affinity components respectively. Evidence was also obtained for the involvement of a temperature-dependent transition, that occurred at around 25 degrees C, in the modulation of malonyl-CoA binding to the mitochondria. The possible physiological roles of the two components of malonyl-CoA binding in relation to the regulation of overt carnitine palmitoyltransferase (CPT I) activity in vivo are discussed.
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Umei, T., K. Takeshige, and S. Minakami. "NADPH binding component of neutrophil superoxide-generating oxidase." Journal of Biological Chemistry 261, no. 12 (April 1986): 5229–32. http://dx.doi.org/10.1016/s0021-9258(19)57201-5.

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Chaoen Xiao, Guangping Zeng, QingChuan Zhang, and Shurong Ning. "Research and Implementation of Distributed Binding SoftMan Component." INTERNATIONAL JOURNAL ON Advances in Information Sciences and Service Sciences 4, no. 23 (December 31, 2012): 565–74. http://dx.doi.org/10.4156/aiss.vol4.issue23.70.

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Schade, Arthur L., and Liona Caroline. "An iron-binding component in human blood plasma." Journal of Trace Elements in Experimental Medicine 14, no. 2 (2001): 111–13. http://dx.doi.org/10.1002/jtra.1017.

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Dissertations / Theses on the topic "Binding component"

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Troup, Timothy J. "A component system architecture to enable user-directed component binding at run-time." Thesis, University of Glasgow, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414045.

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Watson, Keith. "Characterisation of the dextrin 2-sulphate cell surface binding component." Thesis, Imperial College London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265875.

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Whyte, J. "Studies on the alpha-bungarotoxin binding component in human brain." Thesis, University of Bath, 1985. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.352843.

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Maximum levels of alpha-bungarotoxin binding to human foetal brain membranes remained constant between gestational ages of 10-24 weeks at 30-50 gmol/mg protein (1.1-1.5 p mol/g wet weight in whole brain). Subcellular fractionation of whole brain homogenate showed an enrichment of alpha-bungarotoxin binding in the crude mitochondrial fraction and the subsequent nerve ending sub-fraction. The high proportion of occluded LDH in the nerve ending fraction was indicative of the presence of synaptosomes. Analysis of the fraction at the electron microscope level revealed the existence of synaptosome-like profiles containing mitochondria and very few synaptic vesicles. The specific binding of alpha-bungarotoxin to membranes and crude detergent extracts was shown to be saturable with KD (app) of 3.5 x 10-9 and 2.4 x 10-9M respectively. Scatchard and Hill analysis revealed the presence of a single class of sites exhibiting no cooperativity. Association rate constants, determined by measuring the binding of alpha-bungarotoxin with time to membranes and crude detergent extracts were 2.3 x 105M-1 sec-1 and 2.6 x 105M-1 sec-1 respectively. Dissociation of alpha-bungarotoxin from membranes and crude detergent extract was rapid with a T1/2 in the order of 12 min. and off rate constants of 9.2 x 10-4 sec-1 and 9.8 x 10-4 sec -1 respectively. The intrinsic dissociation constant (KD) was calculated from the on and off rate constants to be 4.0 x 10-9M and 3.3 x 10-9M for membranes and crude detergent extracts respectively. Competition studies with several cholinergic ligands indicated that the alpha-bungarotoxin binding sites displayed a predominantly nicotinic pharmacology. Unlabelled a-BgTx, nicotine and d-tubocurarine were found to be the most effective inhibitors of [125I]-alpha-bungarotoxin binding. A histological study employing autoradiographic techniques showed that [125I] -alpha-bungarotoxin binding was concentrated primarily in the dorsal horn of the spinal cord of a 14 weekold foetus. Clusters of silver grains above the background density were also observed in the ventral horn. Silver grain densities above background were abolished by pre-treatment of the sections with unlabelled alpha-bungarotoxin. A sample of myasthenic plasma and the subsequently prepared immunoglobulin fraction containing antibodies directed against the nAChR from human skeletal muscle were able to precipitate a low but measurable amount of the alpha-bungarotoxin binding sites in human foetal brain. Samples of serum in which an anti-(muscle nAChR) titre was absent failed to precipitate the brain component.
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Marafie, Sulaiman. "TRIM7, a novel binding protein of the mTORC2 component Sin1." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/trim7-a-novel-binding-protein-of-the-mtorc2-component-sin1(c344b542-0706-4ec0-be06-ce6683cee52e).html.

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TRIM7 is a member of the TRIM (tripartite motif-containing) protein superfamily. This family has been implicated in many disorders such as genetic diseases, neurological diseases, and cancers. Little is known about the function of TRIM7 except that it interacts with glycogenin and may regulate glycogen biosynthesis. Recently, a yeast two-hybrid protein-protein interaction screen revealed the binding of TRIM7 to Sin1, a protein found in a complex with the mammalian target of rapamycin (mTOR) protein kinase. mTOR can form two complexes, mTORC1 and mTORC2, which are important for cell growth, differentiation, and survival. Sin1 is a core component of mTORC2 and is critical for mTORC2 stability and activity. It was confirmed by co-immunoprecipitation that TRIM7 associates with Sin1 and mTOR in cultured mammalian cells. Furthermore, it was demonstrated that TRIM7 is a phosphoprotein, although it was not directly targeted by mTOR in vitro. Similar to some other TRIM family proteins, it was demonstrated that TRIM7 has a ubiquitin E3 ligase function allowing it to autoubiquitinate both in vitro and in cells. The autoubiquitination of TRIM7 was dependent on its RING domain. Further characterization of TRIM7 indicated that it can both homo-oligomerise as well as hetero-oligomerise with other members of its sub-class of TRIM proteins and that it co-localises with them into discrete cytoplasmic loci. To determine the cellular function of TRIM7, a stable cell line expressing an shRNA directed against TRIM7 was generated. Successful knock down of TRIM7 was achieved and this led to an increase in the protein levels of components of the mTORC2 complex, including Sin1. This coincided with an increase in cell proliferation. In conclusion, this research identifies a novel role for TRIM7 as a ubiquitin ligase involved in regulating cell proliferation and provides a potential link between TRIM7 and the mTOR pathway, a major transducer of proliferative and cell survival signals.
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Ray, Nandita. "Characterisation of an α-bungarotoxin binding component of chick optic lobe." Thesis, Imperial College London, 1990. http://hdl.handle.net/10044/1/46521.

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Christner, Robert B. "Studies on the binding specifications of human serum amyloid P- component /." The Ohio State University, 1993. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487847761305544.

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Vaughan, P. J. "Studies on a component of the herpes simplex virus DNA polymerase." Thesis, University of Leeds, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379648.

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Rodrigues, Daniel Joseph. "Structure-function relationships in the NADP (H) binding component of proton-translocating transhydrogenase from Rhodospirillum rubrum." Thesis, Oxford Brookes University, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289256.

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Grancell, Adam Scott 1969. "Biochemical characterization of CBF3, an essential DNA-binding component of the yeast kinetochore." Thesis, Massachusetts Institute of Technology, 1998. http://hdl.handle.net/1721.1/49652.

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Vinger, Gift. "THE STATUS OF THE PROJECTION PRINCIPLE IN GOVERNMENT-BINDING THEORY." Journal for New Generation Sciences, Vol 6, Issue 2: Central University of Technology, Free State, Bloemfontein, 2008. http://hdl.handle.net/11462/509.

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Published Article
The role of the Projection Principle within Chomsky's Government-Binding (GB) Theory is to preserve the subcategorisation properties of lexical items at all levels of syntactic representation, viz. D-structure, S-structure, and Lexical Form. Arguments have been made that the Projection Principle is a new concept that is simply an extension of theTransformational Component (XFM) and Emonds' Structure-Preserving Constraint (SPC), and that it does not deserve the high status it has been accorded in GB theory. This paper provides evidence, based on sentences involving movement operations, that the Projection Principle is innovative and that it convincingly addresses what theXFMandSPChave failed to address.
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Books on the topic "Binding component"

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Smith, Adam Campbell. Functional deficiency in the 67-kD elastin binding protein is a crucial component of the pathomechanism of costello syndrome. Ottawa: National Library of Canada, 2000.

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Pan, Qun. Two clusters of acidic amino acids at the amino radical-terminus of complement component C4 [alpha]'-chain are important for C2 binding. Ottawa: National Library of Canada, 2000.

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Taniguchi-Sidle, Aiko. Human complement component C3: Mapping of sites of interaction with C3 binding proteins by site-directed mutagenesis. 1995.

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Ebanks, Roger O'Neil. Studies on the localization and characterization of the C5 and C2 binding sites in the fourth component of complement. 1996.

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Güneş, Güliz, and Anikó Lipták, eds. The Derivational Timing of Ellipsis. Oxford University Press, 2022. http://dx.doi.org/10.1093/oso/9780198849490.001.0001.

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This book explores the nature of ellipsis, the core phenomenon that results in various types of omission in sentences. The book has two functions: it provides a state-of-the-art introduction about the approaches to the derivational timing of ellipsis within the popular ‘silent structure’ accounts of ellipsis in the generative syntactic framework; and it provides a collection of novel works investigating at which point linguistic material becomes silenced during the building (the syntactic derivation) and the realization of syntactic structure. The most important topics handled include areas of intensive research within the Minimalist Program, such as structure building, the architecture of grammar, the interaction of the distinct modules interacting with syntax, the order of operations in the postsyntactic component and constraints on binding relations. The chapters also present novel arguments for and against the derivational approaches to ellipsis, the licensing of ellipsis and the phonological constraints on elliptical sentences.
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Mamelak, Daniel. The molecular basis of Hsp70-sulfogalactolipid binding: Structure/function of protein and glycolipid components. 2002.

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Keating, Michael. State and Nation in the United Kingdom. Oxford University Press, 2021. http://dx.doi.org/10.1093/oso/9780198841371.001.0001.

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The United Kingdom is not a nation-state but a political union. It was formed by the coming together, over centuries, of territories which retained their own national identities and institutions. Key questions of demos (the people), telos (the purpose of union), ethos (binding values) and the locus of sovereignty were never definitively resolved. Since 1999, Scotland, Wales and Northern Ireland have had their own self-governing institutions within the Union. Devolution was an effort to stabilize the Union in the face of centrifugal pressures, but it left the same key questions unresolved. The Union is now contested in all four of its component parts and fundamental questions are raised about the meaning of political, social and economic union. Unionism, as doctrine and practice appears to have lost its way, unable to adjust to devolution. Brexit has added to the strains because membership of the European Union provided an external support system for the union of the United Kingdom itself. Yet the UK cannot easily fall apart into its constituent nations, and public opinion still appears largely content with the idea of a plurinational union. There is no definitive answer to the question of state and nation within the United Kingdom.
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Chappel, Marie Suzanne. Localization and characterization of the molecular components contributing to the high affinity Fc gamma receptor binding site within human immunoglobin G. 1994.

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Srinivas, Krishna Ravi. Intellectual Property Rights and the Politics of Food. Edited by Ronald J. Herring. Oxford University Press, 2014. http://dx.doi.org/10.1093/oxfordhb/9780195397772.013.34.

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The legal status of plant genetic resources has been subject to numerous international agreements and laws over the centuries. The “common heritage of mankind” approach enabled free access but proved unworkable because of conflicts over intellectual property rights. The Convention on Biological Diversity (1992) recognized sovereign rights of nations over genetic resources within their territory. The Trade Related Intellectual Property Rights Agreement under auspices of the World Trade Organization mandated intellectual property protection for plant varieties, but synchronizing such rights has proved problematic. Many developing countries have enacted sui generis regimes to comply with TRIPS requirements. The International Union for the Protection of New Varieties of Plants Convention provides models that have changed over time. With the advent of agricultural biotechnology and availability of intellectual property rights for plant components, patents relating to plant genetic resources have increased. As plant genetic resources are subject to many overlapping treaties, the regime governing them is becoming more complex, resulting in inconsistencies and disputes. While the rights of plant breeders and the private seed industry are well protected in formal agreements, the rights of farmers, who have nurtured diversity in plant genetic resources, developed varieties of crops with different traits, and contributed to exchange and conservation of plant genetic resources, are left to the discretion of nation-states. Farmers’ rights are mentioned in many international legal instruments, but no binding treaty or convention mandates protecting and promoting the rights of working farmers.
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E, Vance Dennis, and Vance Jean E, eds. Biochemistry of lipids, lipoproteins, and membranes. Amsterdam: Elsevier, 1991.

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Book chapters on the topic "Binding component"

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Jones, Neil D., and Arne J. Glenstrup. "Program Generation, Termination, and Binding-Time Analysis." In Generative Programming and Component Engineering, 1–31. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/3-540-45821-2_1.

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Lee, Jaejoon, and Kyo C. Kang. "Feature Binding Analysis for Product Line Component Development." In Software Product-Family Engineering, 250–60. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-540-24667-1_18.

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Fuchs, Andreas, Dustin Kern, Christoph Krauß, and Maria Zhdanova. "Securing Electric Vehicle Charging Systems Through Component Binding." In Lecture Notes in Computer Science, 387–401. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-54549-9_26.

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Jeong, Anmo, Seungnam Jeong, Yoonsun Lim, and Myung Kim. "Active Binding Technology: A Reuse-Enabling Component Model." In Lecture Notes in Computer Science, 270–73. Berlin, Heidelberg: Springer Berlin Heidelberg, 2008. http://dx.doi.org/10.1007/978-3-540-68073-4_28.

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Bakos, M. A., A. Kurosky, and R. M. Goldblum. "Characterizing the polymeric immunoglobulin binding region of human secretory component." In Advances in Mucosal Immunology, 298–301. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-1848-1_84.

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Riethman, Harold C., and Louis A. Sherman. "Characterization of a Novel Chlorophyll-Binding Component of Cyanobacterial Thylakoids." In Progress in Photosynthesis Research, 379–82. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3535-8_92.

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Donato, Armando Di, Shiv K. Srivastava, and Juan Carlos Lacal. "Analysis of the Biochemical and Biological Activities of Deletion Mutants of the H-Ras P21 Protein Suggest That Gap is an Essential Component of Its Effector Function." In The Guanine — Nucleotide Binding Proteins, 179–90. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4757-2037-2_17.

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Donato, Dominique M., Steven K. Hanks, Kenneth A. Jacobson, M. P. Suresh Jayasekara, Zhan-Guo Gao, Francesca Deflorian, John Papaconstantinou, et al. "PUMA (p53 Upregulated Modulator of Apoptosis), BBC3 (BCL2-Binding Component 3)." In Encyclopedia of Signaling Molecules, 1520. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4419-0461-4_101126.

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Serban, D., and Ch Rordorf-Adam. "A NEW BINDING SPECIFICITY FOR SERUM AMYLOID P COMPONENT AND ITS PRACTICAL APPLICATIONS." In Proceedings of the Third Symposium, Lyon, France, June 26–28, 1985, edited by Jacques Bienvenu, J. A. Grimaud, and Philippe Laurent, 125–28. Berlin, Boston: De Gruyter, 1986. http://dx.doi.org/10.1515/9783110860757-018.

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Landsmann, P., O. Rosen, M. Pontet, E. G. Shephard, and M. Fridkin. "Human serum amyloid P component (hSAP): Binding to and degradation by human polymorphonuclear leukocytes." In Peptides, 412–13. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0683-2_135.

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Conference papers on the topic "Binding component"

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Li, Jing, Beijun Shen, Yu Zhang, and Yu Zhang. "Process Component Composition for Dynamic Process Adaptation and Lazy Binding." In 2013 Third International Conference on Intelligent System Design and Engineering Applications (ISDEA). IEEE, 2013. http://dx.doi.org/10.1109/isdea.2012.251.

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Franceschinis, G., M. Gribaudo, M. Iacono, S. Marrone, F. Moscato, and V. Vittorini. "Interfaces and Binding in Component Based Development of Formal Models." In 4th International ICST Conference on Performance Evaluation Methodologies and Tools. ICST, 2009. http://dx.doi.org/10.4108/icst.valuetools2009.7677.

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Troup, Timothy J., and Iain Darroch. "A framework to enable user directed component binding at run-time." In Companion of the 18th annual ACM SIGPLAN conference. New York, New York, USA: ACM Press, 2003. http://dx.doi.org/10.1145/949344.949430.

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Zhou, Hang, Xi-min Wang, and Ying-Hua Guo. "A Parallel Computing Component for Linux Cluster with Threads Binding Supports." In 2010 International Conference on Computational Intelligence and Software Engineering (CiSE). IEEE, 2010. http://dx.doi.org/10.1109/cise.2010.5677108.

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Yeom, Gwyduk, Jeonggeum Lee, Choong Kyo Jeong, and Youngkon Lee. "A Binding Framework Using the Dynamic Component Exchange Technique for Heterogeneous Services." In 5th ACIS International Conference on Software Engineering Research, Management & Applications (SERA 2007). IEEE, 2007. http://dx.doi.org/10.1109/sera.2007.5.

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Busse, M., L. Haab, M. Mariam, C. Krick, T. Weis, W. Reith, and D. J. Strauss. "Assessment of aversive stimuli dependent attentional binding by the N170 VEP component." In 2009 Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2009. http://dx.doi.org/10.1109/iembs.2009.5333647.

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Mou, Dongyue, and Daniel Ratiu. "Binding requirements and component architecture by using model-based test-driven development." In 2012 IEEE First International Workshop on the Twin Peaks of Requirements and Architecture (Twin Peaks). IEEE, 2012. http://dx.doi.org/10.1109/twinpeaks.2012.6344557.

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Carvalho-Junior, Francisco Heron, Rafael Dueire Lins, Ricardo Cordeiro Corrês, Gisele Azevedo Araújo, and Jefferson Carvalho Silva. "On the design of abstract binding connectors for high performance computing component models." In the 2007 symposium. New York, New York, USA: ACM Press, 2007. http://dx.doi.org/10.1145/1297385.1297397.

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Prokhorenko, Aleksandr, Yuri Gnidenko, Yuri Chibisov, and Yuri Chibisova. "AB INITIO CALCULATION OF ADSORPTION POSITION EFFECT ON MAGNETIZATION REDISTRIBUTION IN P-DOPED SILICENE." In Mathematical modeling in materials science of electronic component. LCC MAKS Press, 2021. http://dx.doi.org/10.29003/m2490.mmmsec-2021/124-127.

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The behavior (substitution and adsorption) of a phosphorus atom on the surface of silicene is studied using quantum mechanical calculations. The most favorable positions, binding energy and activation of the phosphorus diffusion barrier have been established. The change in the local magnetic moment of the phosphorus atom is described depending on its position and the position of the surrounding silicon elements.
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Skydanenko, Maksym, Yuliia Kurdes, Mykola Kononenko, and Vitaliy Marenok. "Binding Component Selection and Development of Technology for Granulation of Carbon-Containing Mixtures (Graphite)." In International Youth Science Forum “Litteris et Artibus”. Lviv Polytechnic National University, 2018. http://dx.doi.org/10.23939/lea2018.01.151.

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Reports on the topic "Binding component"

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Baden, Daniel G., and Thomas J. Mende. Characterization of the Ptychodiscus brevis Polyether Neurotoxin Binding Component in Excitable Membranes. Fort Belvoir, VA: Defense Technical Information Center, July 1988. http://dx.doi.org/10.21236/ada199525.

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Baden, Daniel G. Characterization of the P. Brevis Polyether Neurotoxin Binding Component in Excitable Membranes. Fort Belvoir, VA: Defense Technical Information Center, September 1991. http://dx.doi.org/10.21236/ada242877.

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Baden, Daniel G. Characterization of the P. brevis Polyether Neurotoxin Binding Component in Excitable Membranes. Fort Belvoir, VA: Defense Technical Information Center, June 1990. http://dx.doi.org/10.21236/ada225682.

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Baden, Daniel G., and Thomas J. Mende. Characterization of the Ptychodiscus brevis Polyether Neurotoxin Binding Component in Excitable Membranes. Fort Belvoir, VA: Defense Technical Information Center, July 1986. http://dx.doi.org/10.21236/ada175510.

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Wisniewski, Michael, Samir Droby, John Norelli, Dov Prusky, and Vera Hershkovitz. Genetic and transcriptomic analysis of postharvest decay resistance in Malus sieversii and the identification of pathogenicity effectors in Penicillium expansum. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7597928.bard.

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Use of Lqh2 mutants (produced at TAU) and rNav1.2a mutants (produced at the US side) for identifying receptor site-3: Based on the fact that binding of scorpion alpha-toxins is voltage-dependent, which suggests toxin binding at the mobile voltage-sensing region, we analyzed which of the toxin bioactive domains (Core-domain or NC-domain) interacts with the DIV Gating-module of rNav1.2a. This analysis was based on the assumption that the dissociation of toxin mutants upon depolarization would vary from that of the unmodified toxin should the substitutions affect a site of interaction with the channel Gating-module. Using a series of toxin mutants (mutations at both domains) and two channel mutants that were shown to reduce the sensitivity to scorpion alpha-toxins, and by comparison of depolarization-driven dissociation of Lqh2 derivatives off their binding site at rNav1.2a mutant channels we found that the toxin Core-domain interacts with the Gating-module of DIV. Details of the experiments and results appear in Guret al (2011). Mapping receptor site 3 at Nav1.2a by extensive channel mutagenesis (Seattle): Since previous studies with photoaffinity labeling and antibody mapping implicated domains I and IV in scorpion alpha-toxin binding, Nav1.2 channel mutants containing substitutions at these extracellular regions were expressed and tested for receptor function by whole-cell voltage clamp. Of a large number of channel mutants, T1560A, F1610A, and E1613A in domain IV had ~5.9-, ~10.7-, and ~3.9-fold lower affinities for the scorpion toxin Lqh2, respectively, and mutant E1613R had 73-fold lower affinity. Toxin dissociation was accelerated by depolarization for both wild-type and mutants, and the rates of dissociation were also increased by mutations T1560A, F1610A and E1613A. In contrast, association rates for these three mutant channels at negative membrane potentials were not significantly changed and were not voltage-dependent. These results indicated that Thr1560 in the S1-S2 loop, Phe1610 in the S3 segment, and Glu1613 in the S3-S4 loop in domain IV participate in toxin binding. T393A in the SS2-S6 loop in domain I also showed a ~3.4-fold lower affinity for Lqh2, indicating that this extracellular loop may form a secondary component of the toxin binding site. Analysis with the Rosetta-Membrane algorithm revealed a three-dimensional model of Lqh2 binding to the voltage sensor in a resting state. In this model, amino acid residues in an extracellular cleft formed by the S1-S2 and S3-S4 loops in domain IV that are important for toxin binding interact with amino acid residues on two faces of the wedge-shaped Lqh2 molecule that are important for toxin action. The conserved gating charges in the S4 transmembrane segment are in an inward position and likely form ion pairs with negatively charged amino acid residues in the S2 and S3 segments (Wang et al 2011; Gurevitz 2012; Gurevitzet al 2013).
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Whitham, Steven A., Amit Gal-On, and Victor Gaba. Post-transcriptional Regulation of Host Genes Involved with Symptom Expression in Potyviral Infections. United States Department of Agriculture, June 2012. http://dx.doi.org/10.32747/2012.7593391.bard.

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Understanding how RNA viruses cause disease symptoms in their hosts is expected to provide information that can be exploited to enhance modern agriculture. The helper component-proteinase (HC-Pro) protein of potyviruses has been implicated in symptom development. Previously, we demonstrated that symptom expression is associated with binding of duplex small-interfering-RNA (duplex-siRNA) to a highly conserved FRNK amino acid motif in the HC-Pro of Zucchini yellow mosaic virus (ZYMV). This binding activity also alters host microRNA (miRNA) profiles. In Turnip mosaic virus (TuMV), which infects the model plant Arabidopsis, mutation of the FRNK motif to FINK was lethal providing further indication of the importance of this motif to HC-Pro function. In this continuation project, our goal was to further investigate how ZYMV and TuMV cause the mis-expression of genes in cucurbits and Arabidopsis, respectively, and to correlate altered gene expression with disease symptoms. Objective 1 was to examine the roles of aromatic and positively charged residues F164RNH and K215RLF adjacent to FR180NK in small RNA binding. Objective 2 was to determine the target genes of the miRNAs which change during HC-Pro expression in infected tissues and transgenic cucumber. Objective 3 was to characterize RNA silencing mechanisms underlying differential expression of host genes. Objective 4 was to analyze the function of miRNA target genes and differentially expressed genes in potyvirus-infected tissues. We found that the charged K/R amino acid residues in the FKNH and KRLF motifs are essential for virus viability. Replacement of K to I in FKNH disrupted duplex-siRNA binding and virus infectivity, while in KRLF mutants duplex-siRNA binding was maintained and virus infectivity was limited: symptomless following a recovery phenomenon. These findings expanded the duplex-siRNA binding activity of HC-Pro to include the adjacent FRNK and FRNH sites. ZYMV causes many squash miRNAs to hyper-accumulate such as miR166, miR390, mir168, and many others. Screening of mir target genes showed that only INCURVATA-4 and PHAVOLUTA were significantly upregulated following ZYMVFRNK infection. Supporting this finding, we found similar developmental symptoms in transgenic Arabidopsis overexpressing P1-HC-Pro of a range of potyviruses to those observed in miR166 mutants. We characterized increased transcription of AGO1 in response to infection with both ZYMV strains. Differences in viral siRNA profiles and accumulation between mild and severe virus infections were characterized by Illumina sequencing, probably due to the differences in HC-Pro binding activity. We determined that the TuMV FINK mutant could accumulate and cause symptoms in dcl2 dcl4 or dcl2 dcl3 dcl4 mutants similar to TuMV FRNK in wild type Arabidopsis plants. These dcl mutant plants are defective in antiviral defenses, and the results show that factors other than HC-ProFRNK motif can induce symptoms in virus-infected plants. As a result of this work, we have a better understanding of the FRNK and FKNH amino acid motifs of HC-Pro and their contributions to the duplex-siRNA binding functions. We have identified plant genes that potentially contribute to infectivity and symptoms of virus infected plants when they are mis-expressed during potyviral infections. The results establish that there are multiple underlying molecular mechanisms that lead viral pathogenicity, some dependent on HC-Pro. The potential benefits include the development of novel strategies for controlling diseases caused by viruses, methods to ensure stable expression of transgenes in genetically improved crops, and improved potyvirus vectors for expression of proteins or peptides in plants.
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Gafni, Yedidya, and Vitaly Citovsky. Inactivation of SGS3 as Molecular Basis for RNA Silencing Suppression by TYLCV V2. United States Department of Agriculture, November 2013. http://dx.doi.org/10.32747/2013.7593402.bard.

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The Israeli isolate of Tomato yellow leaf curl geminivirus(TYLCV-Is) is a major tomato pathogen, causing extensive crop losses in Israel and in the south-eastern U.S. Yet, little is known about the molecular mechanisms of its interaction with tomato cells. One of the most interesting aspects of such interaction is how the invading virus counteracts the RNA silencing response of the plant. In the former BARD project, we have shown that TYLCV-Is V2 protein is an RNA silencing suppressor, and that this suppression is carried out via the interaction of V2 with the SGS3 component of the plant RNA silencing machinery. This reported project was meant to use our data as a foundation to elucidate the molecular mechanism by which V2 affects the SGS3 activity. While this research is likely to have an important impact on our understanding of basic biology of virus-plant interactions and suppression of plant immunity, it also will have practical implications, helping to conceive novel strategies for crop resistance to TYLCV-Is. Our preliminary data in regard to V2 activities and our present knowledge of the SGS3 function suggest likely mechanisms for the inhibitory effect of V2 on SGS3. We have shown that V2 possess structural and functional hallmarks of an F-box protein, suggesting that it may target SGS3 for proteasomal degradation. SGS3 contains an RNA-binding domain and likely functions to protect the cleavage produces of the primary transcript for subsequent conversion to double-stranded forms; thus, V2 may simply block the RNA binding activity of SGS3. V2 may also employ a combination of these mechanisms. These and other possibilities were tested in this reported project.
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Elbaum, Michael, and Peter J. Christie. Type IV Secretion System of Agrobacterium tumefaciens: Components and Structures. United States Department of Agriculture, March 2013. http://dx.doi.org/10.32747/2013.7699848.bard.

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Objectives: The overall goal of the project was to build an ultrastructural model of the Agrobacterium tumefaciens type IV secretion system (T4SS) based on electron microscopy, genetics, and immunolocalization of its components. There were four original aims: Aim 1: Define the contributions of contact-dependent and -independent plant signals to formation of novel morphological changes at the A. tumefaciens polar membrane. Aim 2: Genetic basis for morphological changes at the A. tumefaciens polar membrane. Aim 3: Immuno-localization of VirB proteins Aim 4: Structural definition of the substrate translocation route. There were no major revisions to the aims, and the work focused on the above questions. Background: Agrobacterium presents a unique example of inter-kingdom gene transfer. The process involves cell to cell transfer of both protein and DNA substrates via a contact-dependent mechanism akin to bacterial conjugation. Transfer is mediated by a T4SS. Intensive study of the Agrobacterium T4SS has made it an archetypal model for the genetics and biochemistry. The channel is assembled from eleven protein components encoded on the B operon in the virulence region of the tumor-inducing plasmid, plus an additional coupling protein, VirD4. During the course of our project two structural studies were published presenting X-ray crystallography and three-dimensional reconstruction from electron microscopy of a core complex of the channel assembled in vitro from homologous proteins of E. coli, representing VirB7, VirB9, and VirB10. Another study was published claiming that the secretion channels in Agrobacterium appear on helical arrays around the membrane perimeter and along the entire length of the bacterium. Helical arrangements in bacterial membranes have since fallen from favor however, and that finding was partially retracted in a second publication. Overall, the localization of the T4SS within the bacterial membranes remains enigmatic in the literature, and we believe that our results from this project make a significant advance. Summary of achievements : We found that polar inflations and other membrane disturbances relate to the activation conditions rather than to virulence protein expression. Activation requires low pH and nutrient-poor medium. These stress conditions are also reflected in DNA condensation to varying degrees. Nonetheless, they must be considered in modeling the T4SS as they represent the relevant conditions for its expression and activity. We identified the T4SS core component VirB7 at native expression levels using state of the art super-resolution light microscopy. This marker of the secretion system was found almost exclusively at the cell poles, and typically one pole. Immuno-electron microscopy identified the protein at the inner membrane, rather than at bridges across the inner and outer membranes. This suggests a rare or transient assembly of the secretion-competent channel, or alternatively a two-step secretion involving an intermediate step in the periplasmic space. We followed the expression of the major secreted effector, VirE2. This is a single-stranded DNA binding protein that forms a capsid around the transferred oligonucleotide, adapting the bacterial conjugation to the eukaryotic host. We found that over-expressed VirE2 forms filamentous complexes in the bacterial cytoplasm that could be observed both by conventional fluorescence microscopy and by correlative electron cryo-tomography. Using a non-retentive mutant we observed secretion of VirE2 from bacterial poles. We labeled the secreted substrates in vivo in order detect their secretion and appearance in the plant cells. However the low transfer efficiency and significant background signal have so far hampered this approach.
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Barash, Itamar, and Robert E. Rhoads. Translational Mechanisms that Govern Milk Protein Levels and Composition. United States Department of Agriculture, November 2004. http://dx.doi.org/10.32747/2004.7586474.bard.

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Original objectives: The long term objective of the project is to achieve higher content of protein in the milk of ruminants by modulating the translational machinery in the mammary gland. The first specific aim of the BARD proposal was to characterize responsiveness of various experimental systems to combination of lactogenic hormones and amino acids with particular emphasis on discrimination between the control of total protein synthesis and milk protein synthesis. Based on the results, we planned to proceed by characterizing the stage of protein synthesis in which the stimulation by lactogenic hormones and amino acid occur and finally we proposed to identify which components of the translation machinery are modified. Background to the topic: Milk protein is the most valuable component in milk, both for direct human consumption and for manufacturing cheese and other protein-based products. Attempts to augment protein content by the traditional methods of genetic selection and improved nutritional regimes have failed. The proposal was based on recent results suggesting that the limiting factor for augmenting protein synthesis in the bovine mammary gland is the efficiency of converting amino acids to milk proteins. Major conclusions, solutions, achievements: Insulin and prolactin synergistically stimulate â-casein mRNA translation by cytoplasmatic polyadenylation. The interaction between insulin and prolactin was demonstrated two decades ago as crucial for milk-protein synthesis, but the molecular mechanisms involved were not elucidated. We found in differentiated CID 9 mouse mammary epithelial cells line that insulin and prolactin synergistically increases the rate of milk protein mRNA translation. We focused on â-casein, the major milk protein, and found that the increase in â-casein mRNA translation was reflected in a shift to larger polysomes, indicating an effect on translational initiation. Inhibitors of the PI3K, mTOR, and MAPK pathways blocked insulin-stimulated total protein and â-casein synthesis but not the synergistic stimulation. Conversely, cordycepin, a polyadenylation inhibitor, abolished synergistic stimulation of protein synthesis without affecting insulin-stimulated translation. The poly(A) tract of â-casein mRNA progressively increased over 30 min of treatment with insulin plus prolactin. The 3’-untranslated region of â-casein mRNA was found to contain a cytoplasmic polyadenylation element (CPE), and in reporter constructs, this was sufficient for the translational enhancement and mRNA-specific polyadenylation. Furthermore, insulin and prolactin stimulated phosphorylation of cytoplasmic polyadenylation element binding protein (CPEB) but did not increase cytoplasmic polyadenylation.
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Whitham, Steven A., Amit Gal-On, and Tzahi Arazi. Functional analysis of virus and host components that mediate potyvirus-induced diseases. United States Department of Agriculture, March 2008. http://dx.doi.org/10.32747/2008.7591732.bard.

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The mechanisms underlying the development of symptoms in response to virus infection remain to be discovered in plants. Insight into symptoms induced by potyviruses comes from evidence implicating the potyviral HC-Pro protein in symptom development. In particular, recent studies link the development of symptoms in infected plants to HC-Pro's ability to interfere with small RNA metabolism and function in plant hosts. Moreover, mutation of the highly conserved FRNK amino acid motif to FINK in the HC-Pro of Zucchini yellow mosaic virus (ZYMV) converts a severe strain into an asymptomatic strain, but does not affect virus accumulation in cucurbit hosts. The ability of this FINK mutation to uncouple symptoms from virus accumulation creates a unique opportunity to study symptom etiology, which is usually confounded by simultaneous attenuation of both symptoms and virus accumulation. Our goal was to determine how mutations in the conserved FRNK motif affect host responses to potyvirus infection in cucurbits and Arabidopsis thaliana. Our first objective was to define those amino acids in the FRNK motif that are required for symptoms by mutating the FRNK motif in ZYMV and Turnip mosaic virus (TuMV). Symptom expression and accumulation of resulting mutant viruses in cucurbits and Arabidopsis was determined. Our second objective was to identify plant genes associated with virus disease symptoms by profiling gene expression in cucurbits and Arabidopsis in response to mutant and wild type ZYMV and TuMV, respectively. Genes from the two host species that are differentially expressed led us to focus on a subset of genes that are expected to be involved in symptom expression. Our third objective was to determine the functions of small RNA species in response to mutant and wild type HC-Pro protein expression by monitoring the accumulation of small RNAs and their targets in Arabidopsis and cucurbit plants infected with wild type and mutant TuMV and ZYMV, respectively. We have found that the maintenance of the charge of the amino acids in the FRNK motif of HC-Pro is required for symptom expression. Reduced charge (FRNA, FRNL) lessen virus symptoms, and maintain the suppression of RNA silencing. The FRNK motif is involved in binding of small RNA species including microRNAs (miRNA) and short interfering RNAs (siRNA). This binding activity mediated by the FRNK motif has a role in protecting the viral genome from degradation by the host RNA silencing system. However, it also provides a mechanism by which the FRNK motif participates in inducing the symptoms of viral infection. Small RNA species, such as miRNA and siRNA, can regulate the functions of plant genes that affect plant growth and development. Thus, this binding activity suggests a mechanism by which ZYMVHC-Pro can interfere with plant development resulting in disease symptoms. Because the host genes regulated by small RNAs are known, we have identified candidate host genes that are expected to play a role in symptoms when their regulation is disrupted during viral infections. As a result of this work, we have a better understanding of the FRNK amino acid motif of HC-Pro and its contribution to the functions of HC-Pro, and we have identified plant genes that potentially contribute to symptoms of virus infected plants when their expression becomes misregulated during potyviral infections. The results set the stage to establish the roles of specific host genes in viral pathogenicity. The potential benefits include the development of novel strategies for controlling diseases caused by viruses, methods to ensure stable expression of transgenes in genetically improved crops, and improved potyvirus vectors for expression of proteins or peptides in plants.
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