Relevant bibliographies by topics / Bible

Academic literature on the topic 'Bible'

Author: Grafiati
Published: 4 June 2021
Last updated: 26 July 2024

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Journal articles on the topic "Bible"

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Price, David H. "Hans Holbein the Younger and Reformation Bible Production." Church History 86, no. 4 (December 2017): 998–1040. http://dx.doi.org/10.1017/s0009640717002086.

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Hans Holbein the Younger produced a large corpus of illustrations that appeared in an astonishing variety of Bibles, including Latin Vulgate editions, Desiderius Erasmus's Greek New Testament, rival German translations by Martin Luther and Ulrich Zwingli, the English Coverdale Bible, as well as in Holbein's profoundly influential Icones veteris testamenti (Images of the Old Testament)—to name only his better-known contributions. This essay discusses strategies that the artist developed for accommodating the heterogeneity of the various humanist and Reformation Bibles. For Erasmus's innovative Bibles, Holbein connected the text to the expansive concept of Renaissance humanist art, simultaneously portraying the new Bible and humanist art as part of a broadly defined cultural-philosophical discourse. Similarly, Holbein's production of Protestant Bibles, most importantly the epochal Luther Bible, associated the new text with the humanist Bible and, in so doing, conceptualized the humanist biblical image as a validation of religious art in a new context. Ultimately, the reliance on humanist art as a cultural authority mitigated perception of the heterogeneity of the text to the point that the publishers of Holbein's Icones completely displaced the text with the daring creation of a new genre: the picture Bible. With the exception of the iconography of royal supremacy in England, Holbein's Bible image was exceedingly movable, an artistic efficiency designed to contribute to the stability of the Bible image across a wide humanist and multiconfessional spectrum.
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Fan, Weixia. "A Study on Translation Expressions by Contrasting Chinese and Korean Bibles versions: Focused on 『Chinese Union Version with New Punctuation』 and 『New Korean Revised Version』." K Association of Education Research 9, no. 1 (February 28, 2024): 139–60. http://dx.doi.org/10.48033/jss.9.1.7.

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In this study, looking back on the flow and main translations of the Chinese Bible and Korean Bible translators, especially Chinese Character Bible which had an impact on early Korean Bible translations and even the modern chinese and Korean Bibles. Based on this, we selected the contents of some texts from among the modern Bible translations 『Chinese Union version with New Punctuation』 and 『New Korean Revised Version』, which account for the largest proportion of use in denominations in China, overseas, and Korea respectively, as subjects of comparative studies. Various aspects of vocabulary and grammatical translation expressions related to two traditional translation methods and translation expressions were examined here. This aims to help understand and interpret the style and expression content related to the translation methods of the modern Chinese and Korean Bibles by presenting the linguistic difference between the two languages and also the commonalities and differences in the translation method and translation expression according to the nature of the Bible itself.
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Carroll, Robert P. "He-Bibles and She-Bibles: Reflections On the Violence Done To Texts By Productions of English Translations of the Bible." Biblical Interpretation 4, no. 3 (1996): 257–69. http://dx.doi.org/10.1163/156851596x00013.

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AbstractThe political nature of English Bibles (Geneva Bible, Douai-Rhemes, KingJames Bible) in the long history of biblical translation is often neglected in the analysis of Bibles as ideological weapons ofwar in the theopolitical struggles of the time of their production. The eventual triumph of the KJB centuries later inscribed ideological traces of partisan versions of those struggles in "the English Bible." Violence is done to the biblical text and by readers of the text in the perpetuation of such Bibles as translations. Some examples of these kinds of violence are discussed, with observations on the hermeneutic constraints built into such translations.
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Trigano, Shmuel. "Les bibles de la Bible." Pardès 50, no. 2 (2011): 113. http://dx.doi.org/10.3917/parde.050.0113.

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Chao, Hsing-Hao. "The Battle of Two Bibles: When and How Did the King James Bible Gain Its Popularity over the Geneva Bible?" Renaissance and Reformation 46, no. 2 (January 10, 2024): 71–105. http://dx.doi.org/10.33137/rr.v46i2.42289.

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This article addresses two questions: “When did the King James Bible gain a foothold of popularity among the English people?” and “How did the Geneva Bible lose its popularity to the King James Bible?” By reviewing the post-1611 printing of these two versions of the Bible and examining the texts of the Paul’s Cross sermons and the parliamentary sermons between 1612 and 1643, I find that the King James Bible was already more popular than the Geneva Bible by 1620, and that the rising trend of the popularity of the King James Bible had become irreversible by 1630. By 1640, the battle of the two Bibles was long over. I also refute the assumption that the political authorities’ suppression of the Geneva Bible caused its defeat. Rather, I argue that the decrease in consumer demand for exegetical notes led to the demise of the Geneva Bible.
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Wong, Simon. "Digitization of Bibles in Greater China (1661–1960)." Bible Translator 72, no. 2 (August 2021): 241–54. http://dx.doi.org/10.1177/20516770211013079.

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Bible translations in (or for) Greater China may be classified into three categories: Chinese, Han dialects, and indigenous languages. All these language groups witness translation activities by Protestant missionaries. However, in its earliest history, Bible translation was pioneered by missionaries of Eastern Christianity in the seventh century or even earlier, whereas from the Catholic side, clear historical narrative has recorded Bible translation work in the thirteenth century by John of Montecorvino (1247–1328) into a Tatar language. Sadly, this work was not preserved. The earliest extant Bible translation in this vast area was published in 1661 in the Siriya language of Taiwan. This article reports on two major digitization projects: digitization of old Chinese Bibles (1707–1960), including 51 translations in total, and digitization of Bibles in Han dialects/fangyan and indigenous languages (1661–1960)—about 50 languages (including dialects) and 60 translations. These two projects represent the largest and most systematic full-text digitization of the Bible heritage of the area ever undertaken.
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Tops, Bert. "The Quest for the Early Modern Bible Reader: The Dutch Vorsterman Bible (1533–1534), its Readers and Users." Journal of Early Modern Christianity 6, no. 2 (December 18, 2019): 185–222. http://dx.doi.org/10.1515/jemc-2019-2010.

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Abstract This article investigates a book-archeological approach to early modern Bible reading that maps the complex interactions between the substantive elements of a book (text, paratext, illustrations) on the one hand, and its historical readers and the traces they left on the other. That method is applied to all 43 extant copies of the Dutch Vorsterman Bible of 1533–1534. The editions printed by Willem Vorsterman were for a long time regarded as Protestant. However, the Bibles had the approval of the secular and ecclesiastic authorities and were intended for a Catholic public. The edition of 1533–1534 is a glossed Bible with many historicizing, chronological, linguistic and typological paratextual elements. The former owners of the 43 Bibles and their confessional background are examined. Intended and unintended traces of use give clues to the actual use of the Bible. The article turns at the end to a heavily annotated copy, examining the religious ideas of the annotator and the way in which he used the Bible.
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Burke, Linda. "Jeanette L. Patterson, Making the Bible French: The Bible historiale and the Medieval Lay Reader. Toronto: University of Toronto Press, 2022, 249 pp., 8 b/w ill." Mediaevistik 35, no. 1 (January 1, 2022): 531–34. http://dx.doi.org/10.3726/med.2022.01.136.

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Abstract: Were medieval lay Christians forbidden by the Catholic Church to read the Bible in their mother tongue? Were vernacular Bibles a rarity? If vernacular Bibles flourished, as they did, who were the translators, and how were the ancient books of the Bible reworked to engage the lay man or woman of a time and culture far removed from the ancient world? Where the Church authorities approved of these Bibles, what were the agendas served?
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Parmenter, Dorina Miller. "How the Bible Feels." Postscripts: The Journal of Sacred Texts, Cultural Histories, and Contemporary Contexts 8, no. 1-2 (August 19, 2017): 27–37. http://dx.doi.org/10.1558/post.32589.

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Despite Christian leaders’ insistence that what is important about the Bible are the messages of the text, throughout Christian history the Bible as a material object, engaged by the senses, frequently has been perceived to be an effective object able to protect its users from bodily harm. This paper explores several examples where Christians view their Bibles as protective shields, and will situate those interpretations within the history of the material uses of the Bible. It will also explore how recent studies in affect theory might add to the understanding of what is communicated through sensory engagement with the Bible.
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Kazakov, G. A. "Lexical Aspects of Russian Bible Translations." Nauchnyi dialog, no. 6 (June 24, 2021): 59–77. http://dx.doi.org/10.24224/2227-1295-2021-6-59-77.

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The article is devoted to the study of the lexical aspects of Russian Bible translations of the 19th—21st centuries in comparative coverage and is a continuation of a study pre-viously conducted by reference to English Bibles. A historical overview of the existing Russian translations is given (the Synodal translation and the texts preceding it, the New Testament of Bishop Cassian, the Bible of the World Bible Translation Center, the “Central Asian translation”, the translation of Jehovah’s Witnesses, the Bible of the Inter-national Bible Society, the modern translation of the Russian Bible Society, the “Zaoksky Bible”). Special attention is paid to modern editions. Samples of texts are compared according to the lexical parameters of adaptiveness, terminologicalness, style and literalness. On the basis of this comparison, a classification of the considered translations is proposed, and their typological features and interconnections are established. The lexical nature of translations is interpreted in terms of their sociolinguistic effect (public perception). The data obtained confirms the pattern previously found in the English-language Bibles — the inverse relationship between adaptiveness on the one hand and terminologicalness, high style and literalness of the translation on the other. In terms of lexical characteristics, the Synodal and the “Central Asian” translations differ most from each other, which is probably due to their focus on church tradition and missionary goals, respectively.
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Dissertations / Theses on the topic "Bible"

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Aptroot, Marion Jennifer. "Bible translation as cultural reform : the Amsterdam Yiddish Bibles (1678-1679)." Thesis, University of Oxford, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305811.

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Rangoonwala, Abid. "Bible study on Philippians a leader's guide for Home Bible Fellowhsips /." Theological Research Exchange Network (TREN), 1987. http://www.tren.com.

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Barry, David N. "Home Bible Fellowship leader's guide for inductive Bible study lessons on Colossians." Theological Research Exchange Network (TREN), 1986. http://www.tren.com.

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Fisher, Dale K. "Home Bible Fellowship leader's guide for inductive Bible study lessons on Galatians." Theological Research Exchange Network (TREN), 1987. http://www.tren.com.

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Webster, Tiffany. "When the Bible meets the black stuff : a contextual Bible study experiment." Thesis, University of Sheffield, 2017. http://etheses.whiterose.ac.uk/16501/.

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The original contribution to knowledge that this Biblical Studies research project offers is the experimental analysis of the claim that the Bible reading methodology Contextual Bible Study (CBS) should be grounded in ethnography, not only in practice, but also in writing. This project not only critically evaluates this argument, but it also demonstrates the efficacy of its claims by putting the argument presented into practice. This was done by implementing the suggested methodological refinements of CBS via the design and facilitation of a CBS programme that was grounded in ethnography. This took the form of ethnographically researching coalmining culture in South Derbyshire and conducting a CBS programme with a group of contemporary South Derbyshire coal miners. The findings of this project are significant for the discipline of Biblical Studies as thus far CBS has been used in a manner that fails to recognise the need for ethnographic contextualisation – a need which is twofold. First, it has yet to be widely acknowledged that the processes, methods, and goals of CBS are products of the context that gave birth to it (South Africa) and that in order for CBS to be used appropriately and effectively, it should be contextualised in light of its origins. Secondly, ethnographic contextualisation is also needed to ensure the following: that the researcher using CBS understands as fully as possible the context in which they intend to use CBS; that the CBS programme being developed resonates deeply with those participating in it; and that the final audience of any readings produced via CBS are equally knowledgeable about the context of those participating in the process. This thesis therefore examines critically both ethnography and CBS, and through the employment of reflexivity, incorporates ethnography into CBS not only as its formal prerequisite stage, but in a manner whereby its results are then used to shape and inform the entirety of the CBS research.
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Zebulske, Terry E. "Inductive Bible study methodology." Theological Research Exchange Network (TREN), 1988. http://www.tren.com.

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Beck, William P. "The Bible and archaeology." Theological Research Exchange Network (TREN), 1988. http://www.tren.com.

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Yuen, Ka-yiu Thomas, and 袁家耀. "The Alliance Bible Seminary." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1997. http://hub.hku.hk/bib/B31983741.

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Huish, Davies Margaret Elizabeth. "Byron and the Bible." Thesis, University of Liverpool, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.406821.

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Riser, Chris. "Bible driven youth ministry." Theological Research Exchange Network (TREN), access this title online, 2004. http://www.tren.com/search.cfm?p091-0029.

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Books on the topic "Bible"

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Tišnova, Martin z., Olesch Reinhold, Rothe Hans 1928-, Kyas Vladimír, Stejskal Karel, and Urbánková Emma, eds. Kuttenberger Bibel =: Kutnahorská Bible. Paderborn: F. Schöningh, 1989.

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1928-, Rothe Hans, Scholz Friedrich 1928-, and Bohatcová Mirjam, eds. Kralitzer Bibel =: Kralická Bible : Facsimile. Paderborn: F. Schöningh, 1995.

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1928-, Scholz Friedrich, and Rothe Hans 1928-, eds. Svalováclavská bible =: St. Wenzels-Bibel. Paderborn: F. Schöningh, 2001.

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Graves, Kersey. Bible of Bibles. Kessinger Publishing, 1997.

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Bible (Foreign Bibles). Bible Society (The British and Foreign Bible Socie, 1990.

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Knowles, H. Bible (Foreign Bibles). Bible Society (The British and Foreign Bible Socie, 1990.

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Bible (Foreign Bibles). Bible Society (The British and Foreign Bible Socie, 1990.

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Hanssen, Maurice. Bible (Foreign Bibles). Bible Society (The British and Foreign Bible Socie, 1990.

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Bible (Foreign Bibles). Bible Society (The British and Foreign Bible Socie, 1990.

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Bible (Foreign Bibles). Bible Society (The British and Foreign Bible Socie, 1990.

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Book chapters on the topic "Bible"

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Hahn, Josef, and Dalibor Tureček. "Bible kralická (Česká bible)." In Kindlers Literatur Lexikon (KLL), 1–2. Stuttgart: J.B. Metzler, 2020. http://dx.doi.org/10.1007/978-3-476-05728-0_10541-1.

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Pettis, Jeffrey B. "Bible." In Encyclopedia of Psychology and Religion, 209–11. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-24348-7_72.

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Rodrigues, Edwin. "Bible." In Christianity, 154–63. Dordrecht: Springer Netherlands, 2023. http://dx.doi.org/10.1007/978-94-024-2241-2_61.

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Pettis, Jeffrey B. "Bible." In Encyclopedia of Psychology and Religion, 167–69. Boston, MA: Springer US, 2014. http://dx.doi.org/10.1007/978-1-4614-6086-2_72.

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Popovsky, Mark, Benjamin Beit-Hallahmi, David A. Leeming, Fredrica R. Halligan, Jeffrey B. Pettis, Kalman J. Kaplan, Matthew B. Schwartz, et al. "Bible." In Encyclopedia of Psychology and Religion, 96–97. Boston, MA: Springer US, 2010. http://dx.doi.org/10.1007/978-0-387-71802-6_72.

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Boer, Roland. "Stalin’s Bible." In Stalin: From Theology to the Philosophy of Socialism in Power, 1–18. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-6367-1_1.

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Carpenter, Sarah, John J. McGavin, and Greg Walker. "Verity’s Bible." In Early Performance: Courts and Audiences, 133–45. Abingdon, Oxon ; New York : Routledge, 2020. | Series: Variorum collected studies series: Routledge, 2020. http://dx.doi.org/10.4324/9780429269042-12.

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Keller, Eva. "Bible Study." In The Road to Clarity, 85–116. New York: Palgrave Macmillan US, 2005. http://dx.doi.org/10.1057/9781403977007_6.

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Loomba, Ania, and Jonathan Burton. "The Bible." In Race in Early Modern England, 51–55. New York: Palgrave Macmillan US, 2007. http://dx.doi.org/10.1057/9780230607330_8.

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Coogan, Michael. "Bible and Bibles." In The Bible. Oxford University Press, 2020. http://dx.doi.org/10.1093/wentk/9780199383047.003.0001.

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What is the Bible? The Bible is the sacred scripture of Judaism and Christianity. In its pages we encounter some of the most memorable characters in world literature: Adam and Eve, Cain and Abel, Abraham and Sarah, Jacob, Moses, Samson and Delilah, David and Bathsheba,...
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Conference papers on the topic "Bible"

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Grana, Costantino, Daniele Borghesani, Simone Calderara, and Rita Cucchiara. ""Inside the bible"." In Proceeding of the 1st ACM international conference. New York, New York, USA: ACM Press, 2008. http://dx.doi.org/10.1145/1460096.1460158.

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Kim, Hyoyoung, and Jin Wan Park. "Topics on bible visualization." In SIGGRAPH Asia 2013 Art Gallery. New York, New York, USA: ACM Press, 2013. http://dx.doi.org/10.1145/2542256.2542261.

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Octavianus, Cindy, I. Putu Ayub Darmawan, Maria Lidya Wenas, and Mikha Agus Widiyanto. "Effectiveness of Action Bible Game Board Media to Introduce Bible Characters to Children." In 1st World Conference on Social and Humanities Research (W-SHARE 2021). Paris, France: Atlantis Press, 2022. http://dx.doi.org/10.2991/assehr.k.220402.039.

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Ermakova, Olga S., and Aleksandra N. Livanova. "BIBLE IN LANDSMÅL AS CULTURAL AND HISTORICAL PHENOMENON." In Second Scientific readings in memory of Professor V. P. Berkov. St. Petersburg State University, 2023. http://dx.doi.org/10.21638/11701/9785288063572.

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The article discusses the prerequisites for creating translations of the Bible into Norwegian and the history of its first translation into landsmål. The authors describe the evolution of the Bible translation and incorporation in the translation of Norwegian words and expressions, and show the influence of society in changing the biblical text. Particular attention is given to the impact of the Bible translation on the spread of New Norwegian as a cultural language in present-day Norway. The paper compares excerpts from translations of the Gospel of Mark into Norwegian riksmål (1904) and landsmål (1921) language varieties. The authors demonstrate the difference between formal and functional equivalence approaches to translating biblical texts and show that translators into landsmål, while trying to stick to the original source, considered it much more important to convey the poetry of the biblical language and to make it understandable to recipients. In conclusion, it is noted that Norwegian society accepted the new translation of the Bible, which in turn opened the way for the acceptance of nynorsk (the New Norwegian) as the language of the church.
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Wlochova, Andrea. "THE IMPORTANCE OF THE KRALICE BIBLE." In 5th SGEM International Multidisciplinary Scientific Conferences on SOCIAL SCIENCES and ARTS SGEM2018. STEF92 Technology, 2018. http://dx.doi.org/10.5593/sgemsocialf2018/2.3/s21.022.

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Kanungo, Tapas, and Philip Resnik. "The Bible, truth, and multilingual OCR evaluation." In Electronic Imaging '99, edited by Daniel P. Lopresti and Jiangying Zhou. SPIE, 1999. http://dx.doi.org/10.1117/12.335806.

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Susanto, Budi, Wahju Satria Wibowo, Centaury Harjani, and Koniherawati. "Bible Learning with Board Game for Children." In 2nd International Media Conference 2019 (IMC 2019). Paris, France: Atlantis Press, 2020. http://dx.doi.org/10.2991/assehr.k.200325.006.

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CIUCA, Liviu Bogdan. "Inheritance between the Bible and the Civil Code." In 8th LUMEN International Scientific Conference Rethinking Social Action. Core Values in Practice | RSACVP 2017 | 6-9 April 2017 | Suceava – Romania. LUMEN Publishing House, 2017. http://dx.doi.org/10.18662/lumproc.rsacvp2017.16.

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Hassenfeld, Ziva. "Translating the Bible: How Children Negotiate Religious Ideology." In 2023 AERA Annual Meeting. Washington DC: AERA, 2023. http://dx.doi.org/10.3102/2002797.

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Laksmi, Anak Agung, and Agus Adnyana. "The Phrasal Verbs Found in “New Testament Bible”." In Proceedings of the First International Seminar on Languare, Literature, Culture and Education, ISLLCE, 15-16 November 2019, Kendari, Indonesia. EAI, 2020. http://dx.doi.org/10.4108/eai.15-11-2019.2296275.

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Reports on the topic "Bible"

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Baker, Clara. Bertolt Brecht and the Bible. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.5319.

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Rad, E. A. The Bible as an object of literary study. OFERNIO, March 2023. http://dx.doi.org/10.12731/ofernio.2023.25108.

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Santa, Àngels. La Bible du roman populaire: la revue Le Rocambole. Edicions de la Universitat de Lleida, 2023. http://dx.doi.org/10.21001/luc.25.26.12.

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yu, luyou, jinping yang, xi meng, and yanhua lin. Effectiveness of the gut microbiota-bile acid pathway (BAS) in the treatment of Type 2 diabetes: A protocol for systematic review and meta analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, July 2022. http://dx.doi.org/10.37766/inplasy2022.7.0117.

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Review question / Objective: To systematically evaluate the efficacy of the intestinal microbiome - bile acid pathway (BAS) in the treatment of T2DM. Condition being studied: Bile acids (BAs), an important component of bile, are also metabolites derived from cholesterol and promote intestinal absorption and transportation of dietary lipids . Studies have shown that bile acid receptor agonists can promote glP-1 secretion and improve glucose metabolism in preclinical mouse models of obesity and insulin resistance , which may become a new therapeutic target for Type 2 diabetes. However, no systematic review and meta-analysis has been found on the treatment of type 2 diabetes by intestinal microbiome - bile acid pathway. Therefore, we conducted a systematic review and meta-analysis to evaluate the safety and effectiveness of intestinal microbiome-bile acid pathway in the treatment of type 2 diabetes.
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Hjelm, R. P., J. Mang, A. F. Hofmann, C. Schteingart, H. Alkan-Onyuksel, and S. Ayd. Formation of drug-bearing vesicles in mixed colloids of bile salts and phosphatidylcholine. Office of Scientific and Technical Information (OSTI), November 1997. http://dx.doi.org/10.2172/548669.

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Cole, R. O., M. J. Sepaniak, W. L. Hinze, J. Gorse, and K. Oldiges. Bile salt surfactants in micellar electrokinetic capillary chromatography: Application to hydrophobic molecule separations. Office of Scientific and Technical Information (OSTI), January 1990. http://dx.doi.org/10.2172/6287336.

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Stoyanov, Vladislav, Dragomir Dardanov, Elena Arabadzhieva, Atanas Yonkov, Sasho Bonev, Dimitar Bulanov, Julieta Hristova, Lilia Simonova, Violeta Dimitrova, and Dobrin Svinarov. Diagnostic Significance of Cholangiocarcinoma-associated Carbohydrate Antigen (CCA-CA) and Insulin-like Growth Factor 1 (IGF-1) in Blood Serum and Bile in Patients with Extrahepatic Bile Duct Cancer and Obstructive Jaundice. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, September 2019. http://dx.doi.org/10.7546/crabs.2019.09.17.

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Lyutakov, Ivan, Radislav Nakov, Borislav Vladimirov, Ventsislav Nakov, Anastas Dimov, Boyana Asenova, Milena Chetirska, et al. Diagnostic Accuracy and Predictive Value of Serum Fibroblast Growth Factor 19 (FGF19) and Total Free Fecal Bile Acids as Biomarkers of Bile Acid Malabsorption in Patients with Chronic Diarrhea: a Pilot Study. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, December 2020. http://dx.doi.org/10.7546/crabs.2020.12.16.

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Getsov, Plamen, Sylvie Siminkovitch, Mila Kovacheva-Slavova, Stoyanka Dineva, and Borislav Vladimirov. Anatomical Variations of Bile Ducts. Study of Their Frequency in the Bulgarian Population, Using Magnetic Resonance Cholangiopancreatography. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, June 2020. http://dx.doi.org/10.7546/crabs.2020.06.15.

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Shapira, Roni, Judith Grizzle, Nachman Paster, Mark Pines, and Chamindrani Mendis-Handagama. Novel Approach to Mycotoxin Detoxification in Farm Animals Using Probiotics Added to Feed Stuffs. United States Department of Agriculture, May 2010. http://dx.doi.org/10.32747/2010.7592115.bard.

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Abstract:
T-2 toxin, a toxic product belongs to the trichothecene mycotoxins, attracts major interest because of its severe detrimental effects on the health of human and farm animals. The occurrence of trichothecenes contamination is global and they are very resistant to physical or chemical detoxification techniques. Trichothecenes are absorbed in the small intestine into the blood stream. The hypothesis of this project was to develop a protecting system using probiotic bacteria that will express trichothecene 3-O-acetyltransferase (Tri101) that convert T-2 to a less toxic intermediate to reduce ingested levels in-situ. The major obstacle that we had faced during the project is the absence of stable and efficient expression vectors in probiotics. Most of the project period was invested to screen and isolate strong promoter to express high amounts of the detoxify enzyme on one hand and to stabilize the expression vector on the other hand. In order to estimate the detoxification capacity of the isolated promoters we had developed two very sensitive bioassays.The first system was based on Saccharomyces cerevisiae cells expressing the green fluorescent protein (GFP). Human liver cells proliferation was used as the second bioassay system.Using both systems we were able to prove actual detoxification on living cells by probiotic bacteria expressing Tri101. The first step was the isolation of already discovered strong promoters from lactic acid bacteria, cloning them downstream the Tri101 gene and transformed vectors to E. coli, a lactic acid bacteria strain Lactococcuslactis MG1363, and a probiotic strain of Lactobacillus casei. All plasmid constructs transformed to L. casei were unstable. The promoter designated lacA found to be the most efficient in reducing T-2 from the growth media of E. coli and L. lactis. A prompter library was generated from L. casei in order to isolate authentic probiotic promoters. Seven promoters were isolated, cloned downstream Tri101, transformed to bacteria and their detoxification capability was compared. One of those prompters, designated P201 showed a relatively high efficiency in detoxification. Sequence analysis of the promoter region of P201 and another promoter, P41, revealed the consensus region recognized by the sigma factor. We further attempted to isolate an inducible, strong promoter by comparing the protein profiles of L. casei grown in the presence of 0.3% bile salt (mimicking intestine conditions). Six spots that were consistently overexpressed in the presence of bile salts were isolated and identified. Their promoter reigns are now under investigation and characterization.
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