Dissertations / Theses on the topic 'Beta barrel membrane proteins'
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Arjara, Gitrada Rees Douglas C. Gray Harry B. Richards John. "Refolding a beta-barrel membrane protein /." Diss., Pasadena, Calif. : California Institute of Technology, 2007. http://resolver.caltech.edu/CaltechETD:etd-05292007-061922.
Full textPocanschi, Cosmin Lorin. "Folding and stability of beta-barrel membrane proteins from Gram-negative bacteria." [S.l. : s.n.], 2005. http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-16869.
Full textKlein, Astrid. "A structural analysis of the TOB complex, the insertase for Beta-barrel proteins of the mitochondrial outer membrane." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-150786.
Full textKlein, Astrid [Verfasser], and Angelika [Akademischer Betreuer] Böttger. "A structural analysis of the TOB complex, the insertase for beta-barrel proteins of the mitochondrial outer membrane / Astrid Klein. Betreuer: Angelika Böttger." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2011. http://d-nb.info/1028490461/34.
Full textMiles, Jr George Emmett. "On the structure and assembly of staphylococcal leukocidin: a study of the molecular architecture of beta-barrel pore-forming toxins." Texas A&M University, 2003. http://hdl.handle.net/1969.1/3952.
Full textJorgenson, Matthew Allan. "A tale of two RLPAs : studies of cell division in Escherichia coli and Pseudomonas aeruginosa." Diss., University of Iowa, 2014. https://ir.uiowa.edu/etd/1342.
Full textGarrow, Andrew Gordon. "Search algorithms for transmembrane beta-barrel proteins." Thesis, University of Leeds, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.427773.
Full textPongprayoon, Prapasiri. "Molecular modelling of β-barrel outer membrane proteins." Thesis, University of Oxford, 2010. http://ora.ox.ac.uk/objects/uuid:0ed0c22f-027e-4be1-a64c-0819888bbebc.
Full textSchiller, Stina. "Evolutive In-vitro-Adaption eines thermostabilen ([beta][alpha]8-barrel-Proteins [beta-alpha-8-barrel-Proteins] an die Katalyse einer abiotischen Reaktion." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=970732600.
Full textTran, Thuong Van Du. "Modeling and predicting super-secondary structures of transmembrane beta-barrel proteins." Phd thesis, Ecole Polytechnique X, 2011. http://tel.archives-ouvertes.fr/tel-00647947.
Full textSavojardo, Castrense <1981>. "Machine-learning methods for structure prediction of β-barrel membrane proteins." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2013. http://amsdottorato.unibo.it/5429/.
Full textHagan, Christine Lepicier. "Reconstitution of the E. Coli Membrane \(\beta\)-Barrel Assembly Machine from Purified Components." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10202.
Full textChemistry and Chemical Biology
Shah, Aalok K. "Geometry Of Alpha And Beta Protein Structures." Diss., The University of Arizona, 2015. http://hdl.handle.net/10150/578419.
Full textDamaghi, Mehdi. "Characterizing the Functional and Folding Mechanism of β-barrel Transmembrane Proteins Using Atomic Force Microscope." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-114947.
Full textVincent, Karla Kristine. "Transactivation of Beta 2 Adrenergic Receptor by Bradykinin type 2 Receptor via heterodimerization." Diss., Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/37117.
Full textCohen, Alona. "Studies of regulated membrane trafficking /." Access full-text from WCMC, 2008. http://proquest.umi.com/pqdweb?did=1634379741&sid=7&Fmt=2&clientId=8424&RQT=309&VName=PQD.
Full textBengtsson, Luiza. "Novel integral membrane proteins of the inner nuclear membrane characterization of LUMA native LAP 2[beta] [2beta] complexes /." [S.l. : s.n.], 2002. http://www.diss.fu-berlin.de/2002/100/index.html.
Full textHuang, Eugene Y. "The effect of enforced Notch signaling on TCR beta, positive, and negative selection of developing T cells /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/8350.
Full textMiller, Jennifer Louise. "Engineering an ultra-thermostable β₁-adrenoceptor and its structure determination." Thesis, University of Cambridge, 2012. https://www.repository.cam.ac.uk/handle/1810/252260.
Full textSchmid, Katharina [Verfasser]. "Stability of the Outer Membrane Protein TtoA and Interaction with the Insertase TtOmp85 : FTIR studies on two β -barrel proteins / Katharina Schmid." Konstanz : Bibliothek der Universität Konstanz, 2016. http://d-nb.info/1126968994/34.
Full textTeo, Jia-Ling. "Presenilin-1 and TCF/[beta]-catenin signaling : effects on neuronal differentiation /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/9311.
Full textHäusler, Elisabeth [Verfasser], Franz [Akademischer Betreuer] Hagn, Michael [Gutachter] Sattler, and Franz [Gutachter] Hagn. "NMR structural and biochemical studies on the β-barrel membrane proteins OEP21 and hVDAC1 / Elisabeth Häusler ; Gutachter: Michael Sattler, Franz Hagn ; Betreuer: Franz Hagn." München : Universitätsbibliothek der TU München, 2020. http://d-nb.info/1238781551/34.
Full textWiley, Jesse Carey. "Familial Alzheimer's disease mutations decrease gamma-secretase processing of beta amyloid precurson [sic] protein /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/4985.
Full textLaudon, Hanna. "Functional domains in the Alzheimer's disease-associated presenilin 1 protein /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-085-0/.
Full textNilsberth, Camilla. "Distribution and pathophysiological role of amyloid precursor protein and presenilin 1 : characterization in rats and in vitro studies on the pathogenic arctic mutation /." Stockholm : Karolinska Univ. Press, 2002. http://diss.kib.ki.se/2002/91-7349-329-5/.
Full text陳嘉威 and Ka-wai Patrick Chan. "Transforming growth factor-{221}1 induces cell invasiveness via the downregulation of junctional adhesion molecule-A." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B47151602.
Full textHu, Jing. "Prediction of Protein Function and Functional Sites From Protein Sequences." DigitalCommons@USU, 2009. https://digitalcommons.usu.edu/etd/292.
Full textLi, Xiaoman. "Study on memapsin 2 cleavage properties and its interacting proteins." Oklahoma City : [s.n.], 2010.
Find full textSöderberg, Linda. "Characterization of the Alzheimer's disease-associated clac protein /." Stockholm : Karolinska institutet, 2005. http://diss.kib.ki.se/2005/91-7140-252-7.
Full textAlmeida, Vitor Medeiros. "Identificação de domínios em β-glicosidases GH1 através da análise de sua estabilidade." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-22082016-082333/.
Full textIntroduction and Aims: β-glucosidases from the family GH1 of the glycosil-hidrolases presents a (β/α)8 barrel folding. These proteins are usually classified as single domain, however it has been alternatively proposed that they actually are formed by two \"half barrel\" (β/α)4. Thus, (β/α)8 barrel proteins had evolved from an \"half barrel\" ancestor that underwent a duplication-fusion event. The general goal of this project is the search for the two putative (β/α)4 domains, which form the N- and C-terminal ends of the β-glucosidase A from Thermotoga maritima (bglTm) and β-glucosidase B from Paenibacillus polymyxa (bglB), detecting their presence through the thermal and chemical stability of these (β/α)8 barrel proteins. Results: Site-directed mutagenesis was employed to replace residues forming non-covalent interaction between the putative (β/α)4 domains. DNA segments coding for bglB, bglTm and mutant bglTm were cloned into the pLATE51 expression vector and produced as recombinant proteins in E. coli BL21(DE3). The bglB, bglTm and two mutant bglTm, hereafter called T1 and T2, with 2 and 4 mutations respectively on residues in the interface between the protein halves, were purified. They were stable folded as shown by detecting their catalytic activity upon two different substrates and also by circular dichroism (CD) and tryptophan fluorescence analysis. Nevertheless, T2 showed a decrease in the α-helix content (10 %) in the CD analysis, whereas bglTm and T1 are similar (22 %). The wild-type bglTm and T1 are thermostable, whereas T2 was inactivated after pre-incubation at high temperature (kobs = 0,3 min-1 at 80 °C and 0,06 min-1 at 75 °C). The Differential Scanning Fluorimetry experiments revealed Tm of 42 e 81.7 °C for bglB and T2, respectively, whereas wild-type bglTm and mutant T1 did not showed any thermal transition up to 95 °C. Indeed, the analysis of the thermal stability of T1 and T2 did not reveal any evidence of the putative (β/α)4 domains. Following that, the analysis of the protein denaturation by guanidine hydrochloride showed that the c50 for T2 was reduced (2.4 M), whereas no modification was observed for bglTm and T1 (4,3 and 4,5 M, respectively). In agreement the stability of bglTm and T1 (ΔGH2O = 5,2 kcal/mol) is similar, but it was reduced for T2 (ΔGH2O = 3,5 kcal/mol). The m parameter showed a similar cooperative denaturation for wild-type bglTm and mutants T1 and T2, but no evidence of the independent unfolding of the putative (β/α)4 domains was found. Conclusion: In conclusion, the analysis of the thermal and chemical stability of the bglTm did not reveal the presence of the putative (β/α)4 domains that form the N- and C-terminal end of these β-glucosidase.
Le, Marchand Tanguy. "Protein Dynamics by Solid-State NMR with Ultra-Fast Magic-Angle Spinning : from Microcrystals to Amyloid Fibrils and Membrane Proteins." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSEN023/document.
Full textSolid-state NMR with magic angle spinning (MAS) has emerged as a powerful technique for investigating structure and dynamics of insoluble or poorly soluble biomolecules. A number of approaches has been designed for reconstructing molecular structures from the accurate measurement of internuclear proximities, and for probing motions at atomic resolution over timescales spanning several orders of magnitude. Despite this impressive progress, however, MAS NMR studies are still far from routine. Complete determinations, which are often demonstrated on model microcrystalline preparations, are still rare when it comes to more complex systems such as non-crystalline amyloid fibrils or transmembrane proteins in lipid bilayers. My work aimed at extending the possibilities of MAS NMR for applications on complex biomolecular systems in different aggregation states. For this, I exploited the unique possibilities provided by high magnetic fields (700, 800 and 1000 MHz 1H Larmor frequency) in combination with the newest MAS probes capable of spinning rates exceeding 60 kHz. These experimental conditions al- low to boost the sensitivity of MAS NMR through 1H detection at high resolution and to enrich the palette of probes for protein dynamics. The first part of the thesis reports on my contribution to the development of new strategies for backbone resonance assignment, for structure elucidation, and for investigation of backbone and side-chain dynamics. These methodologies significantly reduce the requirements in terms of experimental time, sample quantities and isotopic labeling, and enlarge the molecular size of systems amenable to NMR analysis. The second part describes the application of 1H detected MAS NMR to evaluate the role of protein dynamics in problems such as amyloid fibril formation and membrane protein function. I first addressed the amyloid fibril formation propensity of human beta-2 microglobulin, the light chain of the major histocompatibility complex I. I performed comparative studies of backbone dynamics of the wild type protein as well as a D76N mutant in crystals, and determined some of the structural features of the fibrillar form. This allowed to identify the presence of pathological folding intermediates and to formulate hypotheses on the mechanism of fibrils formation. Finally, I studied the local and global dynamics of membrane proteins in lipid bilayers. In particular, I investigated the mechanism of action of the alkane trans- porter AlkL from P. putida in lipid bilayers. The measurement of parameters for fast (ps-ns) and slow (μs-ms) backbone dynamics of the protein in presence or in absence of a substrate highlights possible routes for molecular uptake and lays the basis for a more detailed mechanistic understanding of the process
Puig, Gomà-Camps Eduard. "Structural characterization of amyloid beta oligomers with functional links associated to Alzheimer's disease." Doctoral thesis, Universitat de Barcelona, 2019. http://hdl.handle.net/10803/667258.
Full textLa malaltia d'Alzheimer (AD) és la forma més comuna de demència. Va ser descrita per primera vegada el 1906 per Alois Alzheimer. Més endavant, al 1984, George Glenner i Colin Masters van aïllar el pèptid amiloide-beta (Aβ) d'un cervell humà i el van associar a la malaltia. Des de llavors, la hipòtesi amiloide ha estat un tema bastant controvertit discutit entre la comunitat científica. Una possible explicació és l’alta complexitat del sistema a causa de la varietat de formes d’agregació que Aβ pot adoptar. Per tant, entendre els vincles entre l'agregació de proteïnes i la neurotoxicitat, i especialment l'obtenció de les estructures 3D dels agregats responsables de la neurotoxicitat, és clau per dissenyar estratègies diagnòstiques i terapèutiques efectives. Malauradament, aquest tema continua sent un dels problemes pendents més importants. El grup de la Dra. Carulla ha estat treballant en la hipòtesi que l'Aβ interactua amb la membrana cel·lular que condueix a una deshomeostasi iònica. Per estudiar aquest escenari, el grup ha canviat el paradigma i ha tractat Aβ com a proteïna de membrana i aplicant tècniques biofísiques ben establertes per a caracteritzar proteïnes de membrana per tal d’estudiar Aβ. D'aquesta manera, el grup ha demostrat que Aβ és capaç de formar un tipus d'oligòmers en presència de micel·les de detergent que adopten una estructura molt específica i definida amb capacitat de formar porus a través de membranes lipídiques. Es refereixen a aquest tipus d’oligòmers com a oligòmers formadors de porus barril β (βPFO). A la present tesi doctoral, presentem l’estudi realitzat per identificar mitjançant diferents tècniques biofísiques, l'estructura 3D de βPFO. Hem utilitzat detergents per estudiar el procés d’oligomerització en un entorn mimètic de membrana. Les micel·les en comparació amb altres entorns biomimètics basats en lípids, permeten l'aplicació d’estratègies d'espectrometria de masses (MS) i de ressonància magnètica nuclear (RMN) ben establertes, proporcionant així informació estructural d'alta resolució. Atès que l’acumulació de diferents quantitats d’Aβ a la membrana és un escenari plausible en el context de la malaltia, hem utilitzat diferents relacions de micel·les Aβ a detergents ([Aβ]: [M]) per estudiar el paper d’aquesta variable en l’oligomerització. procés d'Aβ.
Chang, Tao-Hsin. "Structural studies of Norrin dependent Wnt/beta-catenin signaling." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:a9f2d687-e8ee-4f26-9a8a-12efb87445ae.
Full textO'Sullivan-Murphy, Bryan M. "Contribution of WFS1 to Pancreatic Beta Cell Survival and Adaptive Alterations in WFS1 Deficiency: A Dissertation." eScholarship@UMMS, 2012. https://escholarship.umassmed.edu/gsbs_diss/590.
Full textLipson, Kathryn L. "The Role of Endoplasmic Reticulum Stress Signaling in Pancreatic Beta Cells: a Dissertation." eScholarship@UMMS, 2008. https://escholarship.umassmed.edu/gsbs_diss/363.
Full textAguilar, Mónica Alejandra Pavez. "Caracterização molecular da resistência aos carbapenêmicos em enterobactérias isoladas em hospitais brasileiros." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-28092009-144325/.
Full textIntroduction: After emergence, and dissemination of extended spectrum β-lactamases (ESBL) in members of the Enterobacteriaceae family, carbapenem antibiotics (imipenem, meropenem, ertapenem) have been the therapy of choice, since they are stable to ESBL hydrolysis. Unfortunately, in 2005, the first fatal case of infection by carbapenem-resistant Klebsiella pneumoniae was related in our country. From this episode, new infection cases, including by other genders of Enterobacteriaceae such as Enterobacter, Providencia and Escherichia, began to appear. Regarding carbapenem resistance mechanisms, expression of carbapenem hydrolyzing enzymes has been worldwide reported, whereas interplay between impermeability and AmpC or ESBL production has been sporadic. Furthermore, integrons and plasmids have been associated with mobilization of genetic determinants. The aim of this study was to characterize the mechanisms of resistance to carbapenems, their genetic mobilization and clonal dissemination in enterobacterial isolates recovered from clinical samples in Brazilian hospitals. Material and methods: 28 imipenem-resistant isolates recovered from 8 hospital centres were studied. Phenotypic profiles were characterized by: i) MIC of carbapenems in the presence/absence of β-lactamase inhibitors; ii) bioassay for β-lactamase production; iii) SDS-PAGE to investigate absence of outer membrane porins (OMPs). Molecular characterization of β-lactamase-mediated resistance was made by PCR and DNA sequencing and their plasmid localization was evaluated by transformation. Finally, epidemiological typing was performed by ERIC-PCR, being confirmed by PFGE. Results: 25 isolates were confirmed as being resistant to imipenem (MIC 8-128 µg/mL), exhibiting a multidrug-resistant profile, including to cefoxitin (MIC90 ≥32 µg/mL). Two main mechanism of resistance were identified: i) hydrolysis of carbapenem by class B (IMP-1-like MBL) and class A (KPC-2) enzymes, (the latter being recently reported in our country), and ii) outer membrane impermeability associated to AmpC enzyme production (plasmid-mediated CMY-2 for E. coli and chromosomal AmpC for E. aerogenes), which was the most prevalent mechanism found. Eighteen of 28 isolates lacked 36kDa OMP, which is responsible for uptake of carbapenem antibiotics. The blaKPC-2 and blaCMY-2 genes were successful transferred to E. coli DH10B, confirming the plasmid location of both genes. Co-production of carbapenemases or AmpC and CTXM enzymes was confirmed in 68% of isolates, and molecular typing showed clonal dissemination of IMP-1-, plasmid AmpC- and chromosomal AmpC-producing isolates. Otherwise, KPC-2-producing isolates were not clonally related. Conclusion: The characterization of resistance mechanisms to carbapenems, in this study, reveals a change in the resistance patterns among Enterobacteriaceae family members in Brazilian hospitals, due to versatility of isolates to acquire new resistance determinants, which it has favoured the adaptation to hostile environments. Lack of 36 kDa OMP was the most frequent resistance mechanism, being associated to co-production of β-lactamases. Finally, molecular typing denote a clonal dissemination of imipenem-resistant isolates in Sao Paulo city, with exception of KPC-2-producing isolates, which have been described in other Brazilian cities, suggesting a horizontal gene transfer.
Fan, Yan, Ping Ping Chen, Ying Li, Kui Cui, Daniel M. Noel, Elizabeth D. Cummins, Daniel J. Peterson, Russell W. Brown, and Meng-Yang Zhu. "Corticosterone Administration up-Regulated Expression of Norepinephrine Transporter and Dopamine Β-Hydroxylase in Rat Locus Coeruleus and Its Terminal Regions." Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etsu-works/955.
Full textArjara, Gitrada. "Refolding a beta-barrel membrane protein." Thesis, 2007. https://thesis.library.caltech.edu/2245/1/01_Title.pdf.
Full textPocanschi, Cosmin L. [Verfasser]. "Folding and stability of β-barrel [beta-barrel] membrane proteins from Gram-negative bacteria / vorgelegt von Cosmin L. Pocanschi." 2006. http://d-nb.info/98292268X/34.
Full textSchiller, Stina [Verfasser]. "Evolutive In-vitro-Adaption eines thermostabilen (βα)8-barrel-Proteins [beta-alpha-8-barrel-Proteins] an die Katalyse einer abiotischen Reaktion / vorgelegt von Stina Schiller." 2004. http://d-nb.info/970732600/34.
Full textDamaghi, Mehdi. "Characterizing the Functional and Folding Mechanism of β-barrel Transmembrane Proteins Using Atomic Force Microscope." Doctoral thesis, 2012. https://tud.qucosa.de/id/qucosa%3A26923.
Full textHayat, Sikander [Verfasser]. "Sequence based methods for the prediction and analysis of the structural topology of transmembrane beta barrel proteins / von Sikander Hayat." 2010. http://d-nb.info/1009599496/34.
Full textNelson, Christopher David. "Characterization of Beta-arrestin-Modulated Lipid Kinase Activities for Diacylglycerol and Phosphatidylinositol 4-Phosphate." Diss., 2007. http://hdl.handle.net/10161/206.
Full textKIM, JUN TAE. "Static and dynamic adsorption of [beta]-lactoglobulin on polymeric membrane surface and development of novel membranes by surface modification." 2007. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17106.
Full textWang, Leo D. "Investigation into the roles of the cytoplasmic tails of Ig-alpha and Ig-beta in B cell development /." 2003. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:3077081.
Full textWilliamson, Ritchie, A. Usardi, D. P. Hanger, and B. H. Anderton. "Membrane-bound beta-amyloid oligomers are recruited into lipid rafts by a fyn-dependent mechanism." 2008. http://hdl.handle.net/10454/6237.
Full text"Identification and characterization of novel FE65-interacting proteins." 2009. http://library.cuhk.edu.hk/record=b5896580.
Full textThesis (M.Phil.)--Chinese University of Hong Kong, 2009.
Includes bibliographical references (leaves 76-88).
Abstract also in Chinese.
Acknowledgement --- p.i
摘要 --- p.iii
List of Abbreviations --- p.iv
List of Figures --- p.vi
List of Tables --- p.vii
Chapter Chapter 1 --- Introduction --- p.1
Chapter 1.1 --- FE65 --- p.1
Chapter 1.1.1 --- FE65 Protein Family and Their Structures --- p.2
Chapter 1.1.1.2 --- PTB domains --- p.5
Chapter 1.1.2 --- Expression Pattern of FE65 Proteins --- p.6
Chapter 1.1.3 --- FE65 Family-Transgenic Animals --- p.7
Chapter 1.1.4 --- Interacting Partners of FE65 --- p.8
Chapter 1.1.4.1 --- "APP, APLPl and APLP2" --- p.9
Chapter 1.1.4.2 --- LRP1 and ApoEr2 --- p.10
Chapter 1.1.4.3 --- c-Abl --- p.11
Chapter 1.1.4.4 --- Mena and EVL --- p.11
Chapter 1.1.4.5 --- Tip60 --- p.12
Chapter 1.1.4.6 --- SET --- p.12
Chapter 1.1.4.7 --- Estrogen Receptor a --- p.13
Chapter 1.1.4.8 --- Teashirt --- p.13
Chapter 1.1.4.9 --- CP2/LSF/LBP1 --- p.13
Chapter 1.1.4.10 --- Dexra sl --- p.14
Chapter 1.1.4.11 --- P2X2-receptor subunit --- p.14
Chapter 1.1.4.12 --- Tau --- p.15
Chapter 1.1.4.13 --- Notchl --- p.15
Chapter 1.1.4.14 --- Alcadein --- p.16
Chapter 1.1.4.15 --- CD95/Fas/Apo -1 ligand --- p.16
Chapter 1.1.4.16 --- p68 subunit of pre -mRNA cleavage and polyadenylation factor Im (p68 CFIm) --- p.17
Chapter 1.1.4.17 --- Ataxinl --- p.17
Chapter 1.1.5.1 --- FE65 as an adaptor protein --- p.20
Chapter 1.1.5.2 --- FE65 and Alzheimer´ةs disease --- p.20
Chapter 1.1.5.3 --- Transcriptional / Post-transcriptional regulation --- p.22
Chapter 1.1.5.4 --- Apoptosis and cell cycle regulation --- p.23
Chapter 1.1.5.5 --- Neuronal positioning and cell migration --- p.23
Chapter 1.1.5.6 --- Learning and memory --- p.25
Chapter 1.2 --- Objectives --- p.26
Chapter Chapter 2 --- Investigation of the interaction between FE65 and Arf6 --- p.27
Chapter 2.1 --- Materials --- p.27
Chapter 2.1.1 --- DNA contructs --- p.27
Chapter 2.1.2 --- Cell culture --- p.27
Chapter 2.1.3 --- Immunoblotting --- p.28
Chapter 2.1.4 --- Miscellaneous --- p.28
Chapter 2.2 --- Methods --- p.29
Chapter 2.2.1 --- Preparation of Escherichia coli competent cells --- p.29
Chapter 2.2.2 --- DNA preparation with Intron Plasmid DNA --- p.30
Chapter 2.2.3 --- DNA preparation with Macherey-Nagel NucleoBond Xtra Midi --- p.30
Chapter 2.2.4 --- DNA preparation by the alkaline lysis method --- p.31
Chapter 2.2.5 --- Spectrophotometric analysis of DNA --- p.32
Chapter 2.2.6 --- Agarose gel electrophoresis --- p.32
Chapter 2.2.7 --- Cell culture and transfection --- p.33
Chapter 2.2.8 --- Bacterial GST-pull down assay --- p.33
Chapter 2.2.9 --- GST-pull down assay for testing direct interaction between FE65 and Arf6 --- p.34
Chapter 2.2.10 --- Mammalian GST-pull down assay --- p.35
Chapter 2.2.11 --- Immunoprecipitation --- p.36
Chapter 2.2.12 --- SDS-PAGE --- p.36
Chapter 2.2.13 --- Immunoblotting --- p.39
Chapter 2.3 --- Results --- p.40
Chapter 2.3.1 --- Interaction between Arf6 and FE65 --- p.40
Chapter 2.3.2 --- Determination of the interacting domain of FE65 with Arf6 --- p.43
Chapter 2.3.3 --- Determination if FE65 and Arf6 interact directly --- p.45
Chapter Chapter 3 --- Production of Antisera against Arf6 and Immunostaining of FE65-Arf6 --- p.47
Chapter 3.1 --- Materials --- p.47
Chapter 3.1.1 --- Protein expression and purification --- p.47
Chapter 3.1.2 --- Immunization and harvest of antisera --- p.48
Chapter 3.1.3 --- Immunostaining --- p.48
Chapter 3.2 --- Methods --- p.48
Chapter 3.2.1 --- Protein expression and purification --- p.48
Chapter 3.2.2 --- Bradford assay --- p.50
Chapter 3.2.3 --- Immunization --- p.50
Chapter 3.2.4 --- Antibody purification --- p.51
Chapter 3.2.5 --- Immunostaining --- p.52
Chapter 3.3 --- Results --- p.53
Chapter 3.3.1 --- Recombinant Arf6 expression and purification --- p.53
Chapter 3.3.2 --- Titering of antisera --- p.57
Chapter 3.3.3 --- Determination of antisera specificity --- p.59
Chapter Chapter 4 --- Discussion --- p.68
Chapter Chapter 5 --- Future Perspectives --- p.73
References --- p.76
Gunasekaran, K. "Stereochemical Analysis On Protein Structures - Lessons For Design, Engineering And Prediction." Thesis, 1997. http://etd.iisc.ernet.in/handle/2005/2140.
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