Academic literature on the topic 'BC2L-C'
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Journal articles on the topic "BC2L-C"
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Mazzotta, Sarah, Giulia Antonini, Francesca Vasile, Emilie Gillon, Jon Lundstrøm, Annabelle Varrot, Laura Belvisi, and Anna Bernardi. "Identification of New L-Fucosyl and L-Galactosyl Amides as Glycomimetic Ligands of TNF Lectin Domain of BC2L-C from Burkholderia cenocepacia." Molecules 28, no. 3 (February 3, 2023): 1494. http://dx.doi.org/10.3390/molecules28031494.
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The inhibition of carbohydrate-lectin interactions is being explored as an efficient approach to anti adhesion therapy and biofilm destabilization, two alternative antimicrobial strategies that are being explored against resistant pathogens. BC2L-C is a new type of lectin from Burkholderia cenocepacia that binds (mammalian) fucosides at the N-terminal domain and (bacterial) mannosides at the C-terminal domain. This double carbohydrate specificity allows the lectin to crosslink host cells and bacterial cells. We have recently reported the design and generation of the first glycomimetic antagonists of BC2L-C, β-C- or β-N-fucosides that target the fucose-specific N-terminal domain (BC2L-C-Nt). The low water solubility of the designed N-fucosides prevented a full examination of this promising series of ligands. In this work, we describe the synthesis and biophysical evaluation of new L-fucosyl and L-galactosyl amides, designed to be water soluble and to interact with BC2L-C-Nt. The protein–ligand interaction was investigated by Saturation Transfer Difference NMR, Isothermal Titration Calorimetry and crystallographic studies. STD-NMR experiments showed that both fucosyl and galactosyl amides compete with α-methyl fucoside for lectin binding. A new hit compound was identified with good water solubility and an affinity for BC2L-C-Nt of 159 μM (ITC), which represents a one order of magnitude gain over α-methyl fucoside. The x-ray structure of its complex with BC2L-C-Nt was solved at 1.55 Å resolution.
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Bermeo, Rafael, Anna Bernardi, and Annabelle Varrot. "BC2L-C N-Terminal Lectin Domain Complexed with Histo Blood Group Oligosaccharides Provides New Structural Information." Molecules 25, no. 2 (January 7, 2020): 248. http://dx.doi.org/10.3390/molecules25020248.
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Lectins mediate adhesion of pathogens to host tissues, filling in a key role in the first steps of infection. Belonging to the opportunistic pathogen Burkholderia cenocepacia, BC2L-C is a superlectin with dual carbohydrate specificity, believed to mediate cross-linking between bacteria and host cells. Its C-terminal domain binds to bacterial mannosides while its N-terminal domain (BCL2-CN) recognizes fucosylated human epitopes. BC2L-CN presents a tumor necrosis factor alpha (TNF-α) fold previously unseen in lectins with a novel fucose binding mode. We report, here, the production of a novel recombinant form of BC2L-CN (rBC2L-CN2), which allowed better protein stability and unprecedented co-crystallization with oligosaccharides. Isothermal calorimetry measurements showed no detrimental effect on ligand binding and data were obtained on the binding of Globo H hexasaccharide and l-galactose. Crystal structures of rBC2L-CN2 were solved in complex with two blood group antigens: H-type 1 and H-type 3 (Globo H) by X-ray crystallography. They provide new structural information on the binding site, of importance for the structural-based design of glycodrugs as new antimicrobials with antiadhesive properties.
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Šulák, Ondřej, Gianluca Cioci, Emilie Lameignère, Viviane Balloy, Adam Round, Irina Gutsche, Lenka Malinovská, et al. "Burkholderia cenocepacia BC2L-C Is a Super Lectin with Dual Specificity and Proinflammatory Activity." PLoS Pathogens 7, no. 9 (September 1, 2011): e1002238. http://dx.doi.org/10.1371/journal.ppat.1002238.
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Bassett, Mark J. "A New Recessive Allele at the C Locus for Seedcoat Color in Common Bean." Journal of the American Society for Horticultural Science 120, no. 6 (November 1995): 896–99. http://dx.doi.org/10.21273/jashs.120.6.896.
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The inheritance of a new allele, cv, at the C locus for seedcoat color was studied in common bean (Phaseolus vulgaris L.) using plant introduction (PI) accession 527774 as the source of cv. The cross PI 527774 (yellow-brown seed) x v BC25-593 (mineral-brown seed) genetic tester stock was studied in F1 and F2 progeny. An F3 selection from the above cross, designated F3 cv G b v, was crossed to 5-593 (a Florida breeding line with black seeds), and the F1, and F2 progeny were analyzed for color segregation. The second hackcross [S-593 x F1 (F3 cv G b v x 5-593)] was investigated in selfed progenies from 32 random BC2-F1 parents. Two of the BC2-F2 progenies were further tested in BC2,-F3. A third hackcross of cv to 5-593 was made and analyzed, and an allelism test of cv B V BC2-F35-593 with the cartridge huff cu BC3 5-593 genetic tester stock confirmed that cv is an allele at C. The gene symbol, cv, is proposed for the new allele because it is only expressed with V and gives a grayish-brown seedcoat. Genotypes with C/cv do not show heterozygous mottling with G B v or G b v, and there is no difference in seedcoat color between C G B v and cv G B v, or between C G b v and cv G b v.
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Chen, Feng, Li Fan, Xun Hou, Chunmei Li, and Zhi-Qian Chen. "Structural, Magnetic and Electronic Properties of 3d Transition-Metal Atoms Adsorbed Monolayer BC2N: A First-principles Study." Materials 12, no. 10 (May 16, 2019): 1601. http://dx.doi.org/10.3390/ma12101601.
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Based on the monolayer BC2N structure, the structural, electronic and magnetic properties of 3d transition metal (TM) atoms (V, Cr, Mn, Fe, Co and Ni) adsorbed on the monolayer BC2N, are studied by using the first principle method. The results show that 3d transition metal atoms are stably adsorbed on the monolayer BC2N. The most stable adsorption sites for V, Cr, and Mn atoms are the hollow adsorption site (H) of BC2N, while the other 3d TM atoms (Fe, Co, Ni) are more readily adsorbed above the C atoms (Tc). The majority of TM atoms are chemically adsorbed on BC2N, whereas Cr and Mn atoms are physically adsorbed on BC2N. Except for Ni, most 3d transition metal atoms can induce the monolayer BC2N magnetization, and the spin-charge density indicated that the magnetic moments of the adsorption systems are mainly concentrated on the TM atoms. Moreover, the introduction of TM atoms can modulate the electronic structure of a single layer of BC2N, making it advantageous for spintronic applications, and for the development of magnetic nanostructures.
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WANG, L., H. S. LIM, and C. K. ONG. "DETERMINATIONS OF UPPER CRITICAL FIELDS IN CONTINUOUS GINZBURG–LANDAU MODEL." International Journal of Modern Physics C 15, no. 06 (July 2004): 783–807. http://dx.doi.org/10.1142/s0129183104006297.
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Novel procedures to determine the parallel upper critical field Bc2 (one-dimensional, 1D) have been proposed within a continuous Ginzburg–Landau model. Unlike conventional methods, where Bc2 is obtained through the determination of the smallest eigenvalue of an appropriate eigen equation, the square of the magnetic field is treated as eigenvalue problems by two procedures so that the upper critical field can be directly deduced. The two procedures proposed are extended to determine the upper critical field in the c–a crystal plane (two-dimensional, 2D) with an arbitrary angle θ tilted from the c-axis. The calculated Bc2 from the two procedures are consistent with each other in both 1D and 2D cases. Moreover, the values of Bc2 near the direction parallel to the layers obtained in the 2D case well approximate the counterparts in the 1D case. The properties of the calculated Bc2 are in reasonably good agreement with existing theories and experiments. The profiles of the order parameters associated with Bc2 for both 1D and 2D cases are Gaussian-like, further validating the methodology proposed.
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Zhao, Y., D. W. He, L. L. Daemen, T. D. Shen, R. B. Schwarz, Y. Zhu, D. L. Bish, et al. "Superhard B–C–N materials synthesized in nanostructured bulks." Journal of Materials Research 17, no. 12 (December 2002): 3139–45. http://dx.doi.org/10.1557/jmr.2002.0454.
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We report here the high-pressure synthesis of well-sintered millimeter-sized bulks of superhard BC2N and BC4N materials in the form of a nanocrystalline composite with diamond-like amorphous carbon grain boundaries. The nanostructured superhard B–C–N material bulks were synthesized under high P–T conditions from amorphous phases of the ball-milled molar mixtures. The synthetic B–C–N samples were characterized by synchrotron x-ray diffraction, high-resolution transmission electron microscope, electron energy-loss spectra, and indentation hardness measurements. These new high-pressure phases of B–C–N compound have extreme hardnesses, second only to diamond. Comparative studies of the high P–T synthetic products of BC2N, BC4N, and segregated phases of diamond + cBN composite confirm the existence of the single B–C–N ternary phases.
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Zhao, Hua, Chunchua Tian, Suna Wang, Dezhi Sun, and Chong Zhang. "The comparable study of isoelectronic-bodies of single-walled B/N nanotubes." Characterization and Application of Nanomaterials 5, no. 1 (January 28, 2022): 39. http://dx.doi.org/10.24294/can.v5i1.1416.
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he structure, thermodynamic stability, ionization potential (IP) and electron affinity (EA) energy level difference (Eg) and tension of lowest unoccupied orbit (LUMO) and highest occupied orbit (HOMO) of armchair single wall carbon nanotubes (C-NTs), BN hybrid carbon nanotubes (BC2N-NTs) and all BN nanotubes (BN-NTs) were systematically studied with AM1 method in this paper. Calculation results show that when n value is constant, (n, n) C-NTs (n = 3,4,5,6) has the largest diameter and BN-NTs has the smallest diameter; (n, n) the values of Eg (HOMO-LUMO) and n of C-NTs and BC2N-NTs are related; POAV analysis shows that different hybrid atoms have different contributions to the hybrid mode of nanotube atoms and the tension of nanotubes.
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Bassett, Mark J. "The Seedcoat Color Genotype of `Prim' and the Manteca and Coscorrón Market Classes of Common Bean." HortScience 34, no. 2 (April 1999): 336–37. http://dx.doi.org/10.21273/hortsci.34.2.336.
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Anecdotal evidence exists for nonflatulence among Chilean Manteca and Coscorrón market classes of common bean (Phaseolus vulgaris L.), and there is an hypothesis that the seedcoat color may be associated with superior digestibility. The inheritance of seedcoat color in `Prim', a Manteca market-class dry bean, was investigated using a protocol employing genetic interpretation of seedcoat colors in the F1 from testcrosses of `Prim' with a series of tester stocks. Most of the genetic tester stocks were constructed previously by backcrossing selected recessive alleles for seedcoat color into a recurrent parent (5-593) with seedcoat color genotype P [C r] D J G B V Rk Asp. The genetic tester stocks included two varieties, `Masterpiece' and `V0687', and testers constructed on the 5-593 background, viz., j BC2 5-593, d j BC2 5-593, asp BC2 5-593, b v BC2 5-593, v BC2 5-593, and cu BC3 5-593. The seedcoat color genotype of `Prim' was found to be P [C r] d j G b vlae. The implications of this genotype for pigment chemistry are discussed.
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JANES, M. E., R. NANNAPANENI, and M. G. JOHNSON. "Identification and Characterization of Two Bacteriocin-Producing Bacteria Isolated from Garlic and Ginger Root†." Journal of Food Protection 62, no. 8 (August 1, 1999): 899–904. http://dx.doi.org/10.4315/0362-028x-62.8.899.
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Two bacteriocin-producing bacterial strains were isolated from garlic and ginger root by the agar overlay method. The bacteria were identified by 16S rRNA sequence analyses and fermentation patterns as Leuconostoc mesenteroides (garlic isolate) and Lactococcus lactis (ginger isolate). The bacteriocins were assigned the names leucocin BC2 and lactocin GI3, respectively. Physiochemical properties and antimicrobial spectra of the bacteriocins were determined by the spot-on-lawn method. Both bacteriocins were inhibited by proteolytic enzymes. Leucocin BC2 exhibited a narrow antimicrobial spectrum, inhibiting only Bacillus, Enterococcus, and Listeria species. Lactocin GI3 had a broader spectrum, inhibiting Bacillus, Clostridium, Listeria, Enterococcus, Leuconostoc, Pediococcus, and Staphylococcus species. Both bacteriocins remained active when heated at 90°C for 15 min or 120°C for 20 min. Leucocin BC2 assayed at 37°C showed an inhibitory activity of 1,600 AU/ml, whereas at 8°C the activity was 12,800 AU/ml. Conversely, lactocin GI3 activity was the same at both assay temperatures. Both bacteriocins remained active over a pH range of 2.0 to 9.0 and in various organic solvents. The activity of leucocin BC2 was increased when treated with 0.5% acetic acid and 0.5% lactic acid, whereas lactocin GI3 activity was decreased with either acid. The molecular mass values were 3.7 kDa for leucocin BC2 and 3.9 kDa for lactocin GI3. These results show that the inhibitory substances produced by the bacteria isolated from garlic and ginger are bacteriocins that appear to be different in some characteristics from previously reported bacteriocins.
Dissertations / Theses on the topic "BC2L-C"
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BERMEO, MALO RAFAEL. "DESIGN, SYNTHESIS AND EVALUATION OF ANTAGONISTS TOWARDS BC2L-C." Doctoral thesis, Università degli Studi di Milano, 2021. http://hdl.handle.net/2434/850474.
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This project aims to antagonize for the first time the superlectin BC2L-C from multi-drug resistant (MDR) pathogen Burkholderia cenocepacia.
MDRs such as Burkholderia cenocepacia have become a hazard in the context of healthcareassociated infections, especially for patients admitted with cystic fibrosis or immunocompromising conditions. As other opportunistic Gram-negative bacteria, this pathogen establishes virulence and biofilms through lectin-mediated adhesion. In particular, the superlectin BC2L-C is believed to cross-link human epithelial cells to B. cenocepacia during pulmonary infection.
With the ultimate goal of inhibiting the interactions between the N-terminal of BC2L-C and its target human oligosaccharides, we aim to design glycomimetic antagonists. Here we report the structural study of the target BC2L-C-N-terminal by X-ray crystallography, followed by the design and synthesis of a modular fucoside library of C- and N-glycomimetics. Lastly, we report the biophysical evaluation of the generated glycomimetics against BC2L-CNter by techniques such as STD-NMR, SPR, ITC, DSC; resulting in a lead structure with satisfactory affinity and two crystal structures of antagonist/lectin complexes.
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LAL, KANHAYA. "STRUCTURE-BASED DESIGN OF GLYCOMIMETIC LIGANDS FOR THE N-TERMINAL DOMAIN OF BC2L-C LECTIN." Doctoral thesis, Università degli Studi di Milano, 2021. http://hdl.handle.net/2434/893211.
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The prevalence of drug-resistant infections has challenged the existing treatment regimen using antibiotics. There is a need to discover and employ alternative and complementary therapies to counteract these life threatening infections. In fast few decades, the use of anti-adhesion molecules targeting virulence factors such as lectins has been proven an attractive approach to counteract the infections by disarming the pathogens. This thesis work aimed to design glycomimetic antagonists of the N-terminal domain of the BC2L-C lectin (BC2L-C-nt) from the drug-resistant pathogen known as B. cenocepacia. We employed a fragment-based approach to design glycomimetic antagonists of the target protein (BC2L-C-nt). The initial studies were focused towards the binding site prediction and target evaluation by computational tools which identified additional druggable regions near the fucoside binding site in BC2L-C-nt. These additional regions have been explored further to evaluate the druggability by employing virtual screening of a small fragment library. This identified an interesting region (region ‘X’) that could host the drug-like fragments by establishing some key interactions. These interactions with the lectin have been confirmed using a group of biophysical techniques, including X-ray crystallography. Remarkably, the binding mode of one of the fragment (KL3) has been validated by X-ray crystallography at high resolution confirming the ability of site X to host drug-like fragments. Further, the fragments have been chemically connected to the fucose core to obtain high-affinity bifunctional glycomimetic ligands. Interestingly, the crystal complexes of BC2L-C-nt with two bifunctional glycomimetic ligands again confirmed the druggability of the identified site, thus also validated the computational predictions. Hence, the first generation of glycomimetic ligands with binding affinities in micromolar range have been successfully designed. These glycomimetics provide further opportunities to design high-affinity ligands. Future studies based on structure-based approaches and robust synthetic routes to synthesize glycomimetics can lead towards the high-affinity ligands as anti-adhesive agents against B. cenocepacia.
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Pohlmeyer, Franziska. "Einfluss verschiedener Reinigungsmethoden auf die Haftkraft rezementierter implantatgetragener Zirkoniumdioxid-Kronengerüste." Doctoral thesis, 2013. http://hdl.handle.net/11858/00-1735-0000-0001-BC2B-C.
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Stetig steigende Ansprüche an ästhetisch ansprechenden festsitzenden Zahnersatz, haben zu einer enormen Entwicklung im Bereich der enossalen Implantologie geführt. Ebenso führte die Weiterentwicklung im Bereich dentaler Keramiken zu einer immer häufigeren Anwendung vollkeramischer Systeme. In der vorliegenden in-vitro-Studie wurde der Einfluss verschiedener Reinigungsmethoden auf die Haftkraft rezementierter implantatgetragener Zirkoniumdioxid-Kronengerüste untersucht. Die untersuchten Parameter waren: manuelle Entfernung der Zementreste mit Hilfe eines Heidemann Spatels, Säuberung durch Ausstrahlung der Suprakonstruktion mit Aluminiumdioxid (Korngröße: 110 μm, 2 bar, 15s Dauer), kombinierte manuelle Säuberung und Ultraschallreinigung (Isopropanolbad 99%ig).
Für den Versuch wurden insgesamt 52 Probekörper mit den drei genannten Parametern, sowie eine Kontrollgruppe mit unbehandelten Zirkoniumdioxid-Käppchen je 13 Mal einem Abzugsversuch unterzogen. Zur Herstellung der Kronengerüste wurden die Titan-Abutments mit dem Cercon® eye Scanner (DeguDent, Hanau) eingescannt und die Zirkoniumdioxid-Käppchen daraufhin digital mit der CAD-Software Cercon® art modelliert und im Labor der Firma DeguDent, Hanau, gefräst. Anschließend wurden die Kronengerüste auf den Abutments mit TempBond® NE der Firma Kerr (Ratstatt, Deutschland) zementiert und in der Universalprüfmaschine Zwick Z007 (Zwick, Ulm) den Abzugsversuchen bis zur Dezementierung unterzogen. Die Auswertung der Abzüge erfolgte mit der Software „testXpert“. Die Ergebnisse zeigten, dass die Kombination aus manueller Reinigung und Ultraschallbad verglichen mit den anderen Methoden zu statistisch signifikant höheren Retentionswerten führte. Zwischen der reinen manuellen Säuberung und der Reinigung durch Ausstrahlen des Kronengerüstes zeigte sich kein klinisch relevanter und signifikanter Unterschied.
Book chapters on the topic "BC2L-C"
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Torrens, Francisco, and Gloria Castellano. "Nanostructures Cluster Models in Solution." In Advances in Systems Analysis, Software Engineering, and High Performance Computing, 221–53. IGI Global, 2014. http://dx.doi.org/10.4018/978-1-4666-6252-0.ch012.
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The existence of Single-Wall C-Nanocones (SWNCs), especially nanohorns (SWNHs), and BC2N/Boron Nitride (BN) analogues in cluster form is discussed in solution in this chapter. Theories are developed based on models bundlet and droplet describing size-distribution function. The phenomena present unified explanation in bundlet in which free energy of (BC2N/BN-)SWNCs involved in cluster is combined from two parts: volume one proportional to the number of molecules n in cluster and surface one, to n1/2. Bundlet enables describing distribution function of (BC2N/BN-)SWNC clusters by size. From geometrical differences bundlet [(BC2N/BN-)SWNCs] and droplet (C60/B15C30N15/B30N30) predict dissimilar behaviours. Various disclination (BC2N/BN-)SWNCs are studied via energetic and structural analyses. Several (BC2N/BN-)SWNC's ends are studied that are different because of closing structure and arrangement type. Packing efficiencies and interaction-energy parameters of (BC2N/BN-)SWNCs/SWNHs are intermediate between C60/B15C30N15/B30N30 and (BC2N/BN-)Single-Wall C-Nanotube (SWNT) clusters: in-between behaviour is expected; however, properties of (BC2N/BN-)SWNCs, especially (BC2N/BN-)SWNHs, are calculated closer to (BC2N/BN-)SWNTs. Structural asymmetry in different (BC2N/BN-)SWNCs characterized by cone angle distinguishes properties of types: P2. BC2N/BN, especially species isoelectronic with C-analogues may be stable.
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"- CLUSTER BUNDLET MODEL OF SINGLE-WALL C, BC2N AND BN NANOTUBES, CONES AND HORNS." In Nanoscience and Computational Chemistry, 292–329. Apple Academic Press, 2013. http://dx.doi.org/10.1201/b16368-14.
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