Dissertations / Theses on the topic 'Barramundi'
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Griffiths, Neil R. "Gill disease in barramundi (Lates calcarifer)." Thesis, Griffiths, Neil R. (2009) Gill disease in barramundi (Lates calcarifer). Masters by Research thesis, Murdoch University, 2009. https://researchrepository.murdoch.edu.au/id/eprint/2434/.
Full textBromage, Erin. "The humoral immune response of Lates calcarifer to Streptococcus iniae." Thesis, Townsville, Qld, 2004. https://researchonline.jcu.edu.au/1007/1/01front.pdf.
Full textBromage, Erin. "The humoral immune response of Lates calcarifer to Streptococcus iniae." Townsville, Qld, 2004. http://eprints.jcu.edu.au/1007/1/01front.pdf.
Full textDeKluyver, Tertius Vitus. "Development of larval barramundi under conditions of intense culture." Thesis, Queensland University of Technology, 1998.
Find full textGibson-Kueh, Susan. "Diseases of Asian seabass (or barramundi), Lates calcarifer Bloch." Thesis, Gibson-Kueh, Susan (2012) Diseases of Asian seabass (or barramundi), Lates calcarifer Bloch. PhD thesis, Murdoch University, 2012. https://researchrepository.murdoch.edu.au/id/eprint/14817/.
Full textcom, cmarshall@tobob, and Carina Rynn Ecremen Marshall. "Evolutionary Genetics of Barramundi (Lates Calcarifer)in the Australian Region." Murdoch University, 2005. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20050421.134447.
Full textYowe, David Langdon. "Molecular cloning and characterisation of the Barramundi growth hormone gene." Thesis, Queensland University of Technology, 1994.
Find full textMarshall, Carina Rynn Ecremen. "Evolutionary genetics of barramundi (Lates calcarifer) in the Australian region." Thesis, Marshall, Carina Rynn Ecremen (2005) Evolutionary genetics of barramundi (Lates calcarifer) in the Australian region. PhD thesis, Murdoch University, 2005. https://researchrepository.murdoch.edu.au/id/eprint/181/.
Full textMarshall, Carina Rynn Ecremen. "Evolutionary genetics of barramundi (Lates calcarifer) in the Australian region." Marshall, Carina Rynn Ecremen (2005) Evolutionary genetics of barramundi (Lates calcarifer) in the Australian region. PhD thesis, Murdoch University, 2005. http://researchrepository.murdoch.edu.au/181/.
Full textSiddik, Muhammad Abu Bakar. "Physiological Responses of Juvenile Barramundi (Lates calcarifer) Fed Processed Animal Protein Diets." Thesis, Curtin University, 2018. http://hdl.handle.net/20.500.11937/75651.
Full textArdiansyah. "Efficacy of duckweed (Lemna minor Linnneus) integrated in barramundi recirculating aquaculture system (RAS)." Thesis, Curtin University, 2018. http://hdl.handle.net/20.500.11937/81910.
Full textYounus, Zakhariya Sona. "Effects of pre and post freezing treatments on barramundi (Lates calcarifer, Bloch) fillet quality." Thesis, Curtin University, 2014. http://hdl.handle.net/20.500.11937/1653.
Full textRussell, David John. "Some aspects of the biology of the Barramundi, Lates calcarifer (Bloch) in Eastern Queensland." Thesis, Queensland University of Technology, 1990. https://eprints.qut.edu.au/35966/1/35966_Russell_1990.pdf.
Full textVo, Binh Van. "Physiological responses of juvenile barramundi, Lates calcarifer (Bloch, 1790) when fed bioprocessed plant base diets." Thesis, Curtin University, 2017. http://hdl.handle.net/20.500.11937/59626.
Full textKinhult, Anne. "Barramundi (Lates calcarifer) IGF-I : characterization of cDNA, genomic sequences, and regulation of mRNA expression." Thesis, Queensland University of Technology, 1996.
Find full textEtzion, Asaf. "The Effect of probiotics on the innate immune system and disease resistance of Barramundi, Lates calcarifer /." Sde Boker [Israel] : Ben-Gurion University of the Negev, 2008. http://aranne5.lib.ad.bgu.ac.il/others/EtzionAsaf.pdf.
Full textau, gavin partridge@challengertafe wa edu, and Gavin Partridge. "Inland Saline Aquaculture: Overcoming Biological and Technical Constraints Towards the Development of an Industry." Murdoch University, 2008. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20081021.100437.
Full textChaklader, Md Reaz. "Supplementing insect meal and fish protein hydrolysates in barramundi, Lates calcarifer diet improves the inclusion efficiency of poultry by-product meal: a physiological approach." Thesis, Curtin University, 2021. http://hdl.handle.net/20.500.11937/86668.
Full textDegger, Brian. "Fish insulin-like growth factors : their role in growth from a functional perspective." Thesis, Queensland University of Technology, 2001.
Find full textWu, Yu-Chi, and 吳育騏. "Persistent Infection of Fish Nodavirus in Barramundi Brain Cell Line and the Antiviral Mechanism of Barramundi Mx Protein." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/72383820037327001933.
Full text國立臺灣大學
動物學研究所
96
The BB cell line derived from the brain tissue of a barramundi (Lates calcarifer) that survived nervous necrosis virus (NNV) infection is persistently infected with NNV. To elucidate whether interferon (IFN) plays a role in the mechanism of NNV-persistent infection in BB cell line, a virus-negative control cell line was obtained by treating BB cells with NNV-specific rabbit antiserum for 5 subcultures. After the treatment, NNV titer or RNA or capsid protein was no longer detected in the cured BB (cBB) cells. Expression of Mx gene, encoding a type I IFN-inducible antiviral protein, was found in BB cells and cBB cells following NNV infection, but not in NNV-free cBB cells. Moreover, expression of Mx gene and antiviral activity against NNV were induced in cBB cells by the treatment with MAb-neutralized BB cell supernatant. Furthermore, NNV persistent infection was induced again in cBB cell culture if multiplicity of infection (MOI) was low (≦1). These experimental results indicated that IFN-like cytokines existed in the culture supernatant of BB cells, and IFN-induced response played an important role in protecting the majority of cells from virus lytic infection and regulating NNV persistence in the BB cell line. We obtained a full-length cDNA clone for the Mx gene of barramundi (Lates calcarifer), using RACE (rapid amplification of cDNA ends) polymerase chain reaction (PCR) amplification of RNA extracted from a barramundi brain cell line cBB. The Mx cDNA of 2.2 kb contains an open reading frame (ORF) of 1875 nucleotides encoding a protein of 624 amino acids. The predicted barramundi Mx protein is 71.4 kDa and contains a tripartite guanosinetriphosphate (GTP)-binding motif at the amino terminal and a leucine zipper at the carboxyl terminal, characteristic of all known Mx proteins. Poly I:C-transfection induced the expression of Mx gene in cBB cells, and the induction level at 28 ℃ was higher than that at 20 ℃. Moreover, Mx gene expression was also induced by viral infection, including fish nodavirus, birnavirus, and iridovirus. Among these, nodavirus was a stronger inducer than the other two viruses. Using an antiviral activity assay, we revealed that poly I:C-transfected cBB cells had antiviral activity against fish nodavirus and birnavirus, but not iridovirus. Furthermore, the replication of nodavirus and birnavirus could be restored after the expression of Mx gene was down-regulated by siRNA. Therefore, these results indicated that the expression of barramundi Mx gene was able to inhibit the proliferation of fish nodavirus and birnavirus. The expression levels of NNV RNA-dependent RNA polymerase (RdRp) and capsid protein were found to increase in NNV-infected cBB cells within 24 h post-infection (hpi), but later decrease as soon as the expression of barramundi Mx protein. Moreover, NNV capsid protein, RNA2, and viral titer all elevated in the cBB cells when barramundi Mx protein expression was down-regulated by siRNA. Barramundi Mx protein was observed to colocalize with NNV RdRp at the perinuclear area in NNV-infected cBB cells by immunofluorescence staining, and was co-precipitated with NNV RdRp by immunoprecipitation assay. Furthermore, the complexes of barramundi Mx protein and NNV RdRp could colocalize with lysosomes, and then NNV RdRp would be degragded by the lysosome system. Therefore, it was suggested that barramundi Mx protein suppressed NNV RNA synthesis by sequestration of NNV RdRp to the perinuclear area for the following degradation in the lysosomes.
Wu, Yu-Chi. "Persistent Infection of Fish Nodavirus in Barramundi Brain Cell Line and the Antiviral Mechanism of Barramundi Mx Protein." 2008. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-2907200803431800.
Full textTruong, Binh. "High pressure processing of Barramundi fish (Lates calcarifer)." Thesis, 2017. http://hdl.handle.net/1959.13/1354648.
Full textHigh pressure processing (HPP) is well-known as an innovative processing technology that can extend shelf-life and improve physicochemical properties of fish muscle during storage. However, research for all potential HPP applications on barramundi fish has not been done before. In this study, HPP was applied prior to conventional freezing to improve physicochemical properties of barramundi muscle and to abate the adverse effects of freezing on barramundi muscle during frozen storage. HPP was also used with or without the combination of chitosan coating to extend the shelf-life of barramundi during chilled storage. HPP was also employed to produce barramundi gels, especially, to reduce salt concentration in these gels. For raw barramundi muscle stored in frozen condition at - 18 °C, application of HPP at 150 MPa and initial temperature of 4 °C for 3 min prior to freezing resulted in barramundi fillet with higher hardness and springiness, lower drip loss and stable pH compared to non-pressurised samples during frozen storage for up to 18 weeks. HPP under these conditions also did not induce cooked appearance and lipid oxidation of barramundi fillet, two major drawbacks in pressurised fish muscle. Thus, HPP prior to freezing may be a good option for barramundi processors. Before chilled storage at 4 ± 1 °C, raw barramundi muscle was treated under 3 different conditions including pressurisation (HPP), chitosan coating and pressurisation (HC) and chitosan coating (CHI). Barramundi samples were investigated on day 1, 8, 15 and 23 during chilled storage. HPP at 200 MPa and 4 ± 1 °C for 3 min significantly improved texture profile, pH, drip loss and TVB-N of barramundi muscle as compared to CHI and control treatment. However, HPP did not delay microbial growth compared to CHI and control treatment. Application of HC treatment significantly hampered the development of TVB-N, microbial growth and improved texture and drip loss of barramundi muscle compared to control, HPP and CHI treatment. However, HC treatment resulted in a cooked appearance and acceleration of lipid oxidation, two major drawbacks in pressurised fish muscle. Pressurisation significantly decreased the activity of protease, but did not reduce the activity of LOX. Microscopic images also showed that the microstructures of pressurised samples were better preserved than the cracked microstructure of unpressurised samples. In this study, HPP was found to be the most favourable treatment for improving the quality of barramundi muscles stored in chilled condition for up to 23 days. For the application of HPP to produce barramundi gels, barramundi minced muscles with 1% and 2% added salt were pressurised at 300, 400 and 500 MPa at ≤ 10 °C and 50 °C for 10 min. Pressure induced barramundi gels (PG) exhibited a lower gel strength and poorer texture such as hardness and springiness as compared to conventional heat induced gels. Comparable water holding capacity to heat induced gels was only obtained at a salt concentration of 2% and at pressures ≥ 400 MPa. SEM images showed a compact network with smoother surface of barramundi minced muscle with 2% added salt and HPP at ≥ 400 MPa as compared to conventional heat induced gels. Gelling properties of barramundi minced muscle with 1.5% and 2 % added salt were assessed after HPP at 300, 400 and 500 MPa at 4 °C (initial temperature) for 10 min and subsequent cooking at 90 °C for 30 min. Whiteness, gel forming ability, water holding capacity, hardness and springiness of the barramundi gels increased as applied pressure and salt concentration increased. At 2% salt concentration, HPP resulted in barramundi gels with higher gel strength and smoother texture as compared to conventional heat induced gels (0.1 MPa, 90 °C for 30). At a reduced salt concentration (1.5%) and HPP at ≥ 400 MPa, the quality (gel strength, water holding capacity, hardness and springiness) of pressurised, cooked gels are comparable to those heat-induced gels with 2% added salt, but the microstructure is smoother. Scanning electron microscope images of pressurised, cooked gels showed a compact network with smoother surface than those of heat-only induced gels. Thus, application of HPP prior to cooking could be an effective method to enable reduced salt concentration in barramundi gels. Barramundi minced muscle with 1% and 2% added salt was gelled by different combinations of pressurisation (300, 400 and 500 MPa at 4 °C for 10 min), cooking (0.1 MPa, 90°C for 30 min) and setting (0.1 MPa, 50 °C for 2 h) to improve their mechanical properties and lower the amount of salt added to barramundi gels. At the low salt concentration of 1%, pressurisation prior to cooking (P - C) treatment resulted in barramundi gels with comparable mechanical properties and water holding capacity to those of conventional heat induced (HI) gels with 2% added salt. At a salt concentration of 2%, pressurisation prior to setting (P - S) and P - C gels exhibited higher mechanical properties and water holding capacity than HI gels. Scanning electron microscope images showed a smooth and dense microstructure of P - C and P - S gels, whereas the microstructure of HI gels is rough and less compact. P - S and P - C treatment can result in higher mechanical and functional properties of barramundi gels at conventional salt concentration (2%) compared to HI gels. P - C treatment can lower the salt concentration added to barramundi gels to 1% which is very significant for health-conscious consumers. To produce a shelf-stable barramundi product by high pressure thermal sterilisation (HPTS), barramundi muscle in a brine was treated at 600 MPa and three different temperatures (90, 110 and 120 °C) for 5 min. Barramundi muscle retorted at Fo = 3.38 was used as control. HPTS at 600 MPa and 110 °C and 120 °C, for 5 min, respectively, produced stable barramundi products, which were stored at room temperature and tested for up to 1 year. Hardness and springiness, of HPTS sterilised barramundi samples were enhanced (i.e. increased) compared to retorted samples. Gumminess, chewiness and cohesiveness of HPTS sterilised barramundi muscle were also similar to retorted samples and did not decrease as processing temperature increased. TBA and pH was also similar in all treatments, except for a significant increase of pH of samples treated at 600 MPa and 90 °C for 5 min after 3 months of storage. In general, HPTS could be a feasible option to produce a sterilised barramundi product with better overall quality and is recommended for more research on other HPTS barramundi products.
Shing, Wu Ming, and 吳明賢. "Study of infectious liver necrosis virus on barramundi (Lates calcarifer)." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/79574977207934129250.
Full text輔仁大學
生命科學系碩士班
92
The first case of suspected viral infection of the giant seaperch (Lates calcarifer, Bloch ) fry, which caused 50-90% mortality, was reported in Thailand in 1970, and cases of viral infections of the cultured giant seaperch followed in Australia and Southeastern Asia. The first virus infection in farmed giant seaperch was reported in Taiwan in 1993 and cases occurred continuously. In 2001, the farmed giant seaperch in south Taiwan appeared poor appetite, ataxia and death. We sampled and dissected some ill fish and found extensive hemorrhagic areas and swollen on the anterior liver, which contained lots of cavities histologically. Under the transmission electronic microscope, there were 160-200 nm hexahedral viral particles, whose electronic compact center was 100-120 nm, and 10 to 20 particles aggregated in tissues. We homogenized the hepatic tissue of ill fish, removed the pellet by centrifugation, filtrated the supernatant through 0.22 m filters, and obtained the fish tissue extract. As we injected the tissue extract into healthy giant seaperch, cavities in hepatic tissues were observed. We tried to replicated the giant seaperch virus from the established grouper cell lines, however, they were not susceptible to the virus containing hepatic extract, and we had to establish giant seaperch cell lines for virus replication. We cultured cells from brain, heart, liver, kidney, spleen and muscle, and obtained four types of giant seaperch brain (sp b) cells, three flat and one spindle, and kidney (giant seaperch kidney, sp k) cells, swim bladder (giant seaperch swim bladder, sp b) cells, as well as muscle (giant seaperch muscle, sp m) cells, which appeared spindle. Characterization of cells revealed that cell lines sp b-7 and sp b-2 showed the best growth rate as cultured in the medium containing 15% fetal bovine serum and incubated at 36 °C. Chromosome analysis of cell line sp sb-2 showed that its chromosome number was 40 to 46. The susceptibility study of giant seaperch cell lines to virus from the liver extract of ill fish showed that sp sb cells appeared cytopathic effect (CPE) as incubated with 101- to 103-fold dilutions of virus. However, no significant CPE observed in other giant seaperch cell lines. To detect virus from the tissue extracts of ill fish and cell extracts which showed CPE by PCR, we designed primers referred to the frog virus 3 (FV3) major capsid protein, however, no PCR product was amplified. It is suggested that this giant seaperch virus is not FV3 related or the virus amount in the tissue extract is not sufficient for PCR amplification.
Matthews, Sue Janet. "The regulation of insulin-like growth factors in barramundi, Lates calcarifer." Thesis, 1997. https://researchonline.jcu.edu.au/33782/1/33782-matthews-1992-thesis.pdf.
Full textBudd, Alyssa. "Epigenetic effects of temperature on sex change in barramundi, Lates calcarifer." Thesis, 2020. https://researchonline.jcu.edu.au/65689/1/JCU_65689_budd_thesis_2020.pdf.
Full textGamage, Kumudu Radampola. "The effect of nutrition on reproductive parameters in male barramundi, Lates calcarifer (Bloch)." Thesis, 2001. https://researchonline.jcu.edu.au/33768/1/33768-gamage-2001-thesis.pdf.
Full textChang, Jia-Rong, and 張家榮. "Establishment of a continuous cell line derived from barramundi (Lates calcarifer ) and its applications." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/52636999813684799231.
Full text國立宜蘭大學
生物技術研究所碩士班
95
Lates calcarifer (bloch) belongs to the Lates genus, Centropomidae family, Perciformes order and Osteichthyes class. This tropical and sub-tropical fish is located in the coastal brackish water throughout Okinawa to the Indian Ocean. Lates calcarifer is massively cultured in Southeastern Asia and Southern Taiwan. Nervous Necrosis Virus (NNV), a member of the Betanodaviridae, is an icosahedral, non-enveloped RNA virus 25-30 nm in diameter. NNV mainly infects juveniles, destroying the nerves of the brain, leading to abnormal swimming and high mortality of infected fish, causing devastating economic impact to the aquaculture industry. Since the larvae of Lates calcarifier are highly susceptible to NNV, this research study developed primary and continuous cell lines from the liver, kidney, and the gas bladder of bloch. The liver and kidney cells were propagated over 90 passages while the gas bladder cell line was propagated over 150 passages, maintained in 5% FBS, L-15 medium, and grew quickly at both 28°C and 32°C. Epinephelus lanceolatus NNV was first used to test these different cell lines for susceptibility and results showed that Lates calcarifer gas bladder (LCGB) was highly susceptible. Then, wild type Plectropomus leopardus Nervous Necrosis Virus (PLNNV) was replicated in LCGB and the viral titer was 107 TCID50/mL. Large quantities of PLNNV was grown in LCGB, collected and purified, serving as the antigen for anti-NNV monoclonal antibodies production in BALB/c mice. After limiting dilution and screening, 3 different hybridomas secreting monoclonal antibodies against NNV were successfully produced. The 3 monoclonal antibodies were confirmed by Western blot and IPMA against purified NNV. Subsequently, cloning of PLNNV capsid gene and expression of recombinant proteins in E. coli was done in pQE30 vector and M15 host. A Western blot of the monoclonal antibodies against the recombinant protein proved all 3 monoclonal antibodies produced in this study were against the NNV capsid protein. These monoclonal antibodies are important tools to have for further researches on Nervous Necrosis Virus.
Ngo, Diu Thi. "Evaluation of canola meal as an aquafeed ingredient for barramundi (Asian seabass; Lates calcarifer)." Thesis, 2014. https://researchonline.jcu.edu.au/41354/1/41354-ngo-2014-thesis.pdf.
Full textJones, Ben C. "Addressing quality deficits in farmed barramundi: optimising flavour and quality through pre-harvest practices." Thesis, 2016. https://researchonline.jcu.edu.au/47453/1/47453-jones-2016-thesis.pdf.
Full textAthauda, A. R. Saman Bandara. "Effect of culture environmental conditions on sex inversion of Asian seabass (barramundi), Lates calcarifer (Bloch)." Thesis, 2014. https://researchonline.jcu.edu.au/45404/1/45404-athauda-2014-thesis.pdf.
Full textHuang, Kun-Shan, and 黃崑山. "A Technical Efficiency Analysis of Taiwan’s Barramundi Production - An Application of the Stochastic Frontier Production Function." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/55565125832863774117.
Full text國立臺灣海洋大學
應用經濟研究所
96
Taiwan was the leading producing country of barramundi in the world between 1990 and 1998. Because of the domestic market could not be effectively developed, barramundi production has been decreasing since 1998, and slumped to the lowest point in 2002. Since 2002, export demand for barramundi has increased and the industry has expanded. In 2005, the production of barramundi in Taiwan has ranked second in the world. This research examines the relationship between input and output in barramundi production using accounting information provided by barramundi farmers. We conducted interviews with the managers of barramundi farms between October and December in 2007. The information on the inputs and output for the barramundi from fry to harvest were collected in Chiayi, Kaohsiung and Pingtung counties. The data period ranged from 5 to 19 months for a total of 80 harvests from 50 farms. We make use of the concept of production frontier suggested by Farrell (1957). The model developed by Battese and Coelli (1995) was used to estimate the stochastic production frontier; this production frontier function was used to analyze the impact the manager's ability on productivity and the sources of inefficiency. Our result shows that the Translog production function has a better goodness of fit in terms of the likelihood ratio than the Cobb-Douglas production function. The estimated value for the return to scale is 0.855, and the average value of technical efficiency is 0.917, with 93.25% of the farms having a value exceeding 0.8. For all farms, results of this study suggest that 99% of technical inefficiency is due to human errors. Among all factors, culture density, water source, depth of the ponds, size of the pond, workshop attendance, land ownership, and export of the production have significant impacts on production efficiency. Based on our results, the best farming density is between 50,000 and 100,000 fries per hectare. Use of either pure freshwater or sea water should be avoided. A mixture of fresh and sea water is most appropriate. The depth of pond is recommended to be higher than 1.5 meters. The best size of the pond is less than 0.5 hectares due to the decreasing return to scale found in this study. We recommend managers to attend related training workshops to obtain more information on the safe level of drug usage and ways to prevent fish diseases. On land ownership, this study finds that farm managers who own the land tend to be more efficient in producing barramundi than those who rent. We recommend that if managers want to run their business long term, they would be better off purchasing the land for production. Finally, for the export markets, the fish size should have to be bigger than those sold in domestic markets and hence fish will have to be fed longer. As the rate of body growth decreases with time, it will be less efficient in production. However, export is a safe way to avoid risk. If prices are not good in domestic market, then a better choice is to extend the feeding time for export. The result on the maximum output per hectare of fish farms after correcting for technical inefficiency factors will provide the fishery authority and related fishery groups information to help increase productive efficiency and achieving production goals in the future. In addition, the estimated technical efficiency values will also serve as the basis for comparison and exchange of experience among fish farms, which in turn will provide less efficient farms directions for improvements; and thereby increase the competitiveness of the barramundi industry in Taiwan.
Nankervis, Leo. "Quantitative and qualitative aspects of the protein nutrition of barramundi (Lates calcarifer) larvae fed formulated foods." Thesis, 2005. https://researchonline.jcu.edu.au/1317/1/01front.pdf.
Full textNankervis, Leo. "Quantitative and qualitative aspects of the protein nutrition of barramundi (Lates calcarifer) larvae fed formulated foods /." 2005. http://eprints.jcu.edu.au/1317.
Full textMuirhead, Elisabeth Knowles. "Polybrominated diphenyl ethers: levels in Townsville sediments, depuration and (anti-)estrogenic effects in Barramundi (Lates calcarifer)." Thesis, 2008. https://researchonline.jcu.edu.au/4778/1/Thesis_front.pdf.
Full textMarc, Adrien François. "Development of advanced reproductive techniques to characterize fertility and accelerate selective breeding in barramundi (Lates calcarifer)." Thesis, 2021. https://researchonline.jcu.edu.au/74221/1/JCU_74221_Marc_2021_thesis.pdf.
Full textChen, Yen-Chun, and 陳讌君. "Characterization of apoptosis induced by grouper iridovirus in two newly established cell lines from Barramundi (Lates calcarifer)." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/01425744912026635231.
Full text國立宜蘭大學
生物技術研究所碩士班
96
This study demonstares the induction of apoptosis in BM (barramundi muscle) and BSB (barramundi swim bladder) cell lines, by grouper iridovirus (GIV) infection. The apoptosis was detected and confirmed through various methods. At MOI of 10, cytopathic effect (CPE) characterized as cell shrinkage and rounding was first observed in both GIV-infected BM and BSB cells at as early as 45 min pi (post-infection). GIV infection appeared to progress more rapidly in BM cells than in BSB cell as suggested by the faster development of CPE in the infected BM cells. Cell viability assay also showed a stronger inhibitory effect on the BM (26.6%) and BSB (54.8%) cells by GIV at 4 hpi. The DNA fragmentation, Annexin V and Hoechst 33258 assays were performed at 45 min pi. The chromatin nick could be observed by TUNEL assay at 2 hpi. The above results demonstrate that BM and BSB cell lines can be triggered apoptosis by GIV. The heat-inactivated GIV and UV-inactivated GIV were used to infect cells. The results showed only UV-inactivated can induce apoptosis. Caspase-3, -8, and -9 activities in the BM- and BSB-infected cells were early activated at 30 min pi., and as the infection goes along, caspase-3, -9 had the maxima activities at 45 min pi. However, caspase-8 had the maxima activity in comparison to the mock-infected cells at 1 hpi. GIV-induce apoptosis was inhibited by a pan-caspase inhibitor, z-VAD-fmk, indicating a caspase-activation pathway. The above results showed that GIV can induce BM and BSB cells apoptosis and the apoptosis pathway is caspase-activation dependent pathway.
Chen, Chin-Wen, and 陳瑾玟. "Study On The Genes Expression of Barramundi (Lates calcarifer)Muscle Cell Line Induced By Grouper Iridovirus Infection." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/12272147415756602881.
Full text國立宜蘭大學
生物技術研究所碩士班
96
By definition, viruses are unable to replicate within a host cell. Virus infection can trigger events that lead to apoptosis, however some viruses are known to encode gene products or induce host gene products that block apoptosis and use the host-cell macro-molecular machinery and energy supplies to replicate. In previous studies, it was suggested that GIV could induced apoptosis in barramundi muscle cells via caspase-3, -8, -9 activation pathway. In this study, a proteomic method was applied to identify alterations in protein expression profile in barramundi muscle cells and being an indicator of apoptosis after GIV infection. From this screening, 7 novel proteins were identfied, including Initiation factor 4A 1B (eIF4A1B), Proteasome 20S, ADP-ribosylation factor 1 (Arf-1), Ribose 5 phosphate isomerase(RPIA), Tyrosine Hydroxylase(TH), RAN binding protein 1, and Phosphoglycerate mutase 1(PGAM1), were overexpressed in GIV-infected barramundi muscle cells. In addition, we also determined these genes expression levels by real-time PCR. The results showed that except Initiation factor 4A 1B (eIF4A1B)but, ADP-ribosylation factor 1(Arf-1), Ribose 5 phosphate isomerase (RPIA)and Tyrosine Hydroxylase (TH)were up regulated conpared to the mock-infected cells. However, the Phosphoglycerate mutase 1(PGAM1) expression only rises slightly is heightened in comparison to the mock-infected cells. Summarized our results indicated that GIV could induce host genes expression level, by GIV infection and supports viral, and replication virus-infected cells of premature death. Our results provide an information for understanding of GIV viral replcation in the host cells and possible mechenisms apoptosis regulatory systems.
Newton, James Raymond. "Investigating the genetics of thermal tolerance and adaptation to temperature amongst populations of Australian barramundi (Lates calcarifer)." Thesis, 2013. https://researchonline.jcu.edu.au/41078/1/41078-newton-2013-thesis.pdf.
Full textChen, Nien-Chi, and 陳念岐. "The effects of water temperature on the growth performance, feed intake and body composition of juvenile barramundi (Lates calcarifer)." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/41997383371054445033.
Full text國立屏東科技大學
水產養殖系所
103
Abstract: The study was to evaluate the effects of water temperature on growth, feed intake and body composition of juvenile barramundi (Lates calcarifer). Triplicate groups of fish (initial wt:10 g) were reared in recirculating system with five temperature (23 °C, 26 °C, 29 °C, 32 °C and 35 °C) for 30 days. Survival (100%) were the same (p > 0.05) among all treatments. Feed intake and specific growth rate (SGR) were the highest (p < 0.05) in 29 °C and 32 °C rearing group, followed by 26 °C and 35 °C rearing group, and the lowest in 23 °C rearing group. Fish reared in 29 °C and 32 °C show lower feed conversion ratio than other treatments. When the water temperature were 29 °C and 32 °C, body lipid content was lower but protein content was higher than other treatments. The results suggest that the adequate rearing temperature are 29 °C and 32 °C.
Hathurusingha, Arachchige Priyantha Indrajith. "Predictive modelling and experimental studies on taste-taint as geosmin (GSM) and 2-methylisoborneol (MIB) in farmed barramundi (Lates calcarifer)." Thesis, 2016. http://hdl.handle.net/2440/98256.
Full textThesis (Ph.D.) -- University of Adelaide, School of Chemical Engineering, 2016.
De, Santis Christian. "Regulation of growth: understanding the myostatin functioning in two important aquaculture species, barramundi (Lates calcarifer) and black tiger shrimp (Penaeus monodon)." Thesis, 2011. https://researchonline.jcu.edu.au/29934/1/29934_DeSantis_2011_thesis.pdf.
Full textBanh, Thi Quyen Quyen. "Sex differentiation of barramundi lates calcarifer – understanding male sexual development and its manipulation through exogenous steroids and non-steroidal aromatase inhibitor." Thesis, 2019. https://researchonline.jcu.edu.au/64547/1/JCU_64547_Banh_2019_thesis.pdf.
Full textEdmunds, Richard C. "Evidence for thermal adaptation among geographically, genetically and thermally distinct populations of the Australian barramundi, Lates calcarifer (Bloch 1790): a multi-level approach." Thesis, 2009. https://researchonline.jcu.edu.au/29298/1/29298_Edmunds_2009_thesis.pdf.
Full textBalston, Jacqueline Marie. "An examination of the impacts of climate variability and climate change on the wild barramundi (Lates calcarifer): a tropical estuarine fishery of north-eastern Queensland, Australia." Thesis, 2007. https://researchonline.jcu.edu.au/2060/1/01front.pdf.
Full textBalston, Jacqueline Marie. "An examination of the impacts of climate variability and climate change on the wild barramundi (Lates calcarifer) : a tropical estuarine fishery of north-eastern Queensland, Australia /." 2007. http://eprints.jcu.edu.au/2060.
Full textBarlow, Christopher G. "Aspects of the biology of juvenile barramundi Lates calcarifer (Bloch) relevant to production for recreational fisheries and farming, with a note on the proposal to introduce Nile perch Lates niloticus (L.) to Australia." Thesis, 1998. https://researchonline.jcu.edu.au/24097/1/01front.pdf.
Full textDomingos, Jose A. S. "Filling in the gaps impeding the instigation of selective breeding programs in barramundi Lates calcarifer: fate of genetic diversity through to harvest, estimation of genetic parameters and early prediction of family growth based on cellular processes." Thesis, 2014. https://researchonline.jcu.edu.au/33907/1/39220-jose-domingos-2014-thesis.pdf.
Full text(9816128), Sabiha Marine. "Short-term socio-ecological effects of a localised change in commercial fishing pressure in Queensland, Australia." Thesis, 2021. https://figshare.com/articles/thesis/Short-term_socio-ecological_effects_of_a_localised_change_in_commercial_fishing_pressure_in_Queensland_Australia/15121584.
Full text(2930712), Ivor G. Stuart. "Assessment of a vertical-slot fishway for non-salmonid fish at a tidal barrier on the sub-tropical Fitzroy River, Queensland." Thesis, 1999. https://figshare.com/articles/thesis/Assessment_of_a_vertical-slot_fishway_for_non-salmonid_fish_at_a_tidal_barrier_on_the_sub-tropical_Fitzroy_River_Queensland/20459520.
Full textFishways designed for salmonids in temperate rivers have often been successful, but similar fishways provided for non-salmonid species in tropical and sub- tropical rivers have frequently failed. In 1970 a salmonid style fishway was built on a tidal barrage on the sub -tropical Fitzroy River, in Queensland, north-eastern Australia. Despite modifications to the design in 1987 assessment of the fishway in 1978 and 1992 demonstrated that it was ineffective; particularly as few barramundi (Lates calcarifer [Centropomidae]) were found at the top of the fishway. The inadequacy of the design appeared to be related to very high water velocities and turbulence within the fishway pools. Consequently, since construction of the tidal barrage many catadromous fish populations (species that live in freshwater but breed in saltwater) have declined in the freshwater reaches of the Fitzroy River.
In 1994 the fishway was modified to a vertical -slot design. The study reported in this thesis is an assessment of the effectiveness of the new design from 1995 to 1997. In 38 paired samples taken at the top and bottom of the fishway, over 16 months, 29 fish species and over 23,000 fish were collected at a maximum rate of 3,400 per day. At the top of the fishway 15,471 individuals were collected and 7594 at the bottom. The migratory population consisted of 4 marine species, 9 diadtomous species and 16 potamodromous species. Common species using the fishway included blue -catfish (Arius graeffei [Ariidae]), bony herring (Nematalosa erebi [Clupeidae]), striped mullet (Mugil cephalus [Mugilidae]), barramundi, and long -finned eels (Anguilla reinhardtii [Anguillidae]). Shrimp Macrobrachium australiense [Palaemonidae]), juvenile crabs (Varuna litterata [Grapsidae]) and long -finned elvers did not ascend the full length of the fishway and specific fishways for these species are recommended. Fish between 25 and 640 mm in length ascended the fishway, although the passage of smaller size classes of immature fish was restricted which may be important for the sustainability of these migratory populations. The bulk of the biomass (71% at the top and 66% at the bottom) was made up of the diadromous blue -catfish of which 81 % were immature. Furthermore, a number of other common fish species were represented mostly by juveniles, including striped mullet, bony herring and barramundi.
This study is the first record of barramundi successfully migrating through an in situ fishway in that no significant difference was found between the size of fish at the top and bottom. A major finding was that 95% of the barramundi from the fishway were immature fish (200-640 mm long), but significantly larger barramundi (maximum length 980 mm) were captured by angling near the base of the fishway. Nevertheless, enlargement of vertical -slot width from 0.15 m to 0.45 m only encouraged a small number of larger fish (890 mm maximum length) to enter. Interestingly, very small barramundi (45-163 mm long) were also absent from the fishway, but these were found in a small tidal tributary below the barrage. Very young barramundi appear to inhabit specific off - channel habitat before migrating in the main river at a larger size. The implications of these results are that 150 mm slot widths are suitable for barramundi and that different life stages have different migratory requirements.