Journal articles: 'Barnacle cement proteins'

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Kamino, Kei, Koji Inoue, Tadashi Maruyama, Nobuhiko Takamatsu, Shigeaki Harayama, and Yoshikazu Shizuri. "Barnacle Cement Proteins." Journal of Biological Chemistry 275, no. 35 (September 2000): 27360–65. http://dx.doi.org/10.1016/s0021-9258(19)61519-x.

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Domínguez-Pérez, Dany, Daniela Almeida, Josef Wissing, André M. Machado, Lothar Jänsch, Luís Filipe Castro, Agostinho Antunes, Vitor Vasconcelos, Alexandre Campos, and Isabel Cunha. "The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, Pollicipes pollicipes." International Journal of Molecular Sciences 21, no. 7 (April 5, 2020): 2524. http://dx.doi.org/10.3390/ijms21072524.

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Adhesive secretion has a fundamental role in barnacles’ survival, keeping them in an adequate position on the substrate under a variety of hydrologic regimes. It arouses special interest for industrial applications, such as antifouling strategies, underwater industrial and surgical glues, and dental composites. This study was focused on the goose barnacle Pollicipes pollicipes adhesion system, a species that lives in the Eastern Atlantic strongly exposed intertidal rocky shores and cliffs. The protein composition of P. pollicipes cement multicomplex and cement gland was quantitatively studied using a label-free LC-MS high-throughput proteomic analysis, searched against a custom transcriptome-derived database. Overall, 11,755 peptide sequences were identified in the gland while 2880 peptide sequences were detected in the cement, clustered in 1616 and 1568 protein groups, respectively. The gland proteome was dominated by proteins of the muscle, cytoskeleton, and some uncharacterized proteins, while the cement was, for the first time, reported to be composed by nearly 50% of proteins that are not canonical cement proteins, mainly unannotated proteins, chemical cues, and protease inhibitors, among others. Bulk adhesive proteins accounted for one-third of the cement proteome, with CP52k being the most abundant. Some unannotated proteins highly expressed in the proteomes, as well as at the transcriptomic level, showed similar physicochemical properties to the known surface-coupling barnacle adhesive proteins while the function of the others remains to be discovered. New quantitative and qualitative clues are provided to understand the diversity and function of proteins in the cement of stalked barnacles, contributing to the whole adhesion model in Cirripedia.

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Schultzhaus, Janna N., William Judson Hervey, Chris R. Taitt, Chris R. So, Dagmar H. Leary, Kathryn J. Wahl, and Christopher M. Spillmann. "Comparative analysis of stalked and acorn barnacle adhesive proteomes." Open Biology 11, no. 8 (August 2021): 210142. http://dx.doi.org/10.1098/rsob.210142.

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Barnacles interest the scientific community for multiple reasons: their unique evolutionary trajectory, vast diversity and economic impact—as a harvested food source and also as one of the most prolific macroscopic hard biofouling organisms. A common, yet novel, trait among barnacles is adhesion, which has enabled a sessile adult existence and global colonization of the oceans. Barnacle adhesive is primarily composed of proteins, but knowledge of how the adhesive proteome varies across the tree of life is unknown due to a lack of genomic information. Here, we supplement previous mass spectrometry analyses of barnacle adhesive with recently sequenced genomes to compare the adhesive proteomes of Pollicipes pollicipes (Pedunculata) and Amphibalanus amphitrite (Sessilia). Although both species contain the same broad protein categories, we detail differences that exist between these species. The barnacle-unique cement proteins show the greatest difference between species, although these differences are diminished when amino acid composition and glycosylation potential are considered. By performing an in-depth comparison of the adhesive proteomes of these distantly related barnacle species, we show their similarities and provide a roadmap for future studies examining sequence-specific differences to identify the proteins responsible for functional differences across the barnacle tree of life.

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Yan, Guoyong, Jin Sun, Zishuai Wang, Pei-Yuan Qian, and Lisheng He. "Insights into the Synthesis, Secretion and Curing of Barnacle Cyprid Adhesive via Transcriptomic and Proteomic Analyses of the Cement Gland." Marine Drugs 18, no. 4 (March 31, 2020): 186. http://dx.doi.org/10.3390/md18040186.

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Barnacles represent one of the model organisms used for antifouling research, however, knowledge regarding the molecular mechanisms underlying barnacle cyprid cementation is relatively scarce. Here, RNA-seq was used to obtain the transcriptomes of the cement glands where adhesive is generated and the remaining carcasses of Megabalanus volcano cyprids. Comparative transcriptomic analysis identified 9060 differentially expressed genes, with 4383 upregulated in the cement glands. Four cement proteins, named Mvcp113k, Mvcp130k, Mvcp52k and Mvlcp1-122k, were detected in the cement glands. The salivary secretion pathway was significantly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the differentially expressed genes, implying that the secretion of cyprid adhesive might be analogous to that of saliva. Lysyl oxidase had a higher expression level in the cement glands and was speculated to function in the curing of cyprid adhesive. Furthermore, the KEGG enrichment analysis of the 352 proteins identified in the cement gland proteome partially confirmed the comparative transcriptomic results. These results present insights into the molecular mechanisms underlying the synthesis, secretion and curing of barnacle cyprid adhesive and provide potential molecular targets for the development of environmentally friendly antifouling compounds.

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Tilbury, Maura A., Sean McCarthy, Magdalena Domagalska, Thomas Ederth, Anne Marie Power, and J. Gerard Wall. "The expression and characterization of recombinant cp19k barnacle cement protein from Pollicipes pollicipes." Philosophical Transactions of the Royal Society B: Biological Sciences 374, no. 1784 (September 9, 2019): 20190205. http://dx.doi.org/10.1098/rstb.2019.0205.

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Adhesive proteins of barnacle cement have potential as environmentally friendly adhesives owing to their ability to adhere to various substrates in aqueous environments. By understanding the taxonomic breath of barnacles with different lifestyles, we may uncover commonalities in adhesives produced by these specialized organisms. The 19 kDa cement protein (cp19k) of the stalked barnacle Pollicipes pollicipes was expressed in Escherichia coli BL21 to investigate its adhesive properties. Initial expression of hexahistidine-tagged protein (rPpolcp19k-his) yielded low levels of insoluble protein. Co-overproduction of E. coli molecular chaperones GroEL-GroES and trigger factor (TF) increased soluble protein yields, although TF co-purified with the target protein (TF-rPpolcp19k-his). Surface coat analysis revealed high levels of adsorption of the TF-rPpolcp19k-his complex and of purified E. coli TF on both hydrophobic and hydrophilic surfaces, while low levels of adsorption were observed for rPpolcp19k-his. Tag-free rPpolcp19k protein also exhibited low adsorption compared to fibrinogen and Cell-Tak controls on hydrophobic, neutral hydrophilic and charged self-assembled monolayers under surface plasmon resonance assay conditions designed to mimic the barnacle cement gland or seawater. Because rPpolcp19k protein displays low adhesive capability, this protein is suggested to confer the ability to self-assemble into a plaque within the barnacle cement complex. This article is part of the theme issue ‘Transdisciplinary approaches to the study of adhesion and adhesives in biological systems’.

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Domínguez-Pérez, Dany, Daniela Almeida, Josef Wissing, André M. Machado, Lothar Jänsch, Agostinho Antunes, Luís Filipe Castro, Vitor Vasconcelos, Alexandre Campos, and Isabel Cunha. "Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle Lepas anatifera." International Journal of Molecular Sciences 22, no. 7 (March 25, 2021): 3370. http://dx.doi.org/10.3390/ijms22073370.

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We focus on the stalked goose barnacle L. anatifera adhesive system, an opportunistic less selective species for the substrate, found attached to a variety of floating objects at seas. Adhesion is an adaptative character in barnacles, ensuring adequate positioning in the habitat for feeding and reproduction. The protein composition of the cement multicomplex and adhesive gland was quantitatively studied using shotgun proteomic analysis. Overall, 11,795 peptide sequences were identified in the gland and 2206 in the cement, clustered in 1689 and 217 proteinGroups, respectively. Cement specific adhesive proteins (CPs), proteases, protease inhibitors, cuticular and structural proteins, chemical cues, and many unannotated proteins were found, among others. In the cement, CPs were the most abundant (80.5%), being the bulk proteins CP100k and -52k the most expressed of all, and CP43k-like the most expressed interfacial protein. Unannotated proteins comprised 4.7% of the cement proteome, ranking several of them among the most highly expressed. Eight of these proteins showed similar physicochemical properties and amino acid composition to known CPs and classified through Principal Components Analysis (PCA) as new CPs. The importance of PCA on the identification of unannotated non-conserved adhesive proteins, whose selective pressure is on their relative amino acid abundance, was demonstrated.

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Cleverley, Robert, David Webb, Stuart Middlemiss, Phillip Duke, Anthony Clare, Keiju Okano, Colin Harwood, and Nick Aldred. "In Vitro Oxidative Crosslinking of Recombinant Barnacle Cyprid Cement Gland Proteins." Marine Biotechnology 23, no. 6 (October 29, 2021): 928–42. http://dx.doi.org/10.1007/s10126-021-10076-x.

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AbstractBarnacle adhesion is a focus for fouling-control technologies as well as the development of bioinspired adhesives, although the mechanisms remain very poorly understood. The barnacle cypris larva is responsible for surface colonisation. Cyprids release cement from paired glands that contain proteins, carbohydrates and lipids, although further compositional details are scant. Several genes coding for cement gland-specific proteins were identified, but only one of these showed database homology. This was a lysyl oxidase-like protein (lcp_LOX). LOX-like enzymes have been previously identified in the proteome of adult barnacle cement secretory tissue. We attempted to produce recombinant LOX in E. coli, in order to identify its role in cyprid cement polymerisation. We also produced two other cement gland proteins (lcp3_36k_3B8 and lcp2_57k_2F5). lcp2_57k_2F5 contained 56 lysine residues and constituted a plausible substrate for LOX. While significant quantities of soluble lcp3_36k_3B8 and lcp2_57k_2F5 were produced in E. coli, production of stably soluble lcp_LOX failed. A commercially sourced human LOX catalysed the crosslinking of lcp2_57k_2F5 into putative dimers and trimers, and this reaction was inhibited by lcp3_36k_3B8. Inhibition of the lcp_LOX:lcp2_57k_2F5 reaction by lcp3_36k_3B8 appeared to be substrate specific, with no inhibitory effect on the oxidation of cadaverine by LOX. The results demonstrate a possible curing mechanism for barnacle cyprid cement and, thus, provide a basis for a more complete understanding of larval adhesion for targeted control of marine biofouling and adhesives for niche applications.

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Kamino, K., S. Odo, and T. Maruyama. "Cement Proteins of the Acorn-Barnacle, Megabalanus rosa." Biological Bulletin 190, no. 3 (June 1996): 403–9. http://dx.doi.org/10.2307/1543033.

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Naldrett, Michael J. "The importance of sulphur cross-links and hydrophobic interactions in the polymerization of barnacle cement." Journal of the Marine Biological Association of the United Kingdom 73, no. 3 (August 1993): 689–702. http://dx.doi.org/10.1017/s0025315400033221.

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Solidified adhesive (cement) of three balanid barnacles (Crustacea: Cirripedia) was dissolved using different concentrations of sodium dodecyl sulphate containing a reducing agent. The proteins were separated using SDS-PAGE and blotted onto polyvinylidene difluoride membrane for sequencing. Commonly occurring bands in the cement of each species were identified. One particular protein of 39 kD, found in the cement of Balanus perforatus, has the following N-terminal sequence: TYFPVLSYG?SSSLAPVI, where the? is most likely cysteine. Quinones were not identified in the cement by either infra-red, ultraviolet-visible or solid-state nuclear magnetic resonance spectroscopy and the successful dissolution and sequencing of cement proteins mitigates against their presence. Cement contains a mixture of highly hydrophobic proteins which are cross-linked through cysteine residues. It is the combination of these two components which makes cement highly resistant to chemical degradation. As a result bacteria are usually absent from the cement, possibly further excluded from the porous core of the cement by its smooth outer crust.

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KAMINO, Kei. "Novel barnacle underwater adhesive protein is a charged amino acid-rich protein constituted by a Cys-rich repetitive sequence." Biochemical Journal 356, no. 2 (May 24, 2001): 503–7. http://dx.doi.org/10.1042/bj3560503.

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Barnacle cement is an underwater adhesive that is used for permanent settlement, and is an insoluble protein complex. A method for rendering soluble the cement of Megabalanus rosa has been developed, and three major proteins have been identified in a previous study. To survey the M. rosa cement proteins in a lower molecular mass range, the cement proteins were separated by reversed-phase HPLC and a previously unidentified protein named 20kDa M. rosa cement protein (Mrcp-20k) was found. Mrcp-20k cDNA was cloned to reveal its primary structure. This cDNA was 902bp long and encoded a 202 amino acid-long open reading frame, including 19 amino acids of the signal sequence. The molecular mass in the disulphide form was calculated to be 20357Da and the isoelectric point of the mature polypeptide was 4.72. Mrcp-20k was characterized by an abundance of Cys residues and charged amino acids. The most common amino acid was Cys (17.5%), with Asp (11.5%), Glu (10.4%) and His (10.4%) following in order of magnitude. The alignment of the Cys residues indicated the primary structure of this protein to consist of six degenerated repeats, each about 30 residues long. Mrcp-20k has no intermolecular disulphide bonds and no free thiol groups of Cys in the insoluble cement complex. Abundant Cys is thought to play a role in maintaining the topology of charged amino acids on the molecular surface by intramolecular disulphide-bond formation. The possible function of abundant charged amino acids, including the interaction with a variety of surface metals on the substratum, is discussed.

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Cleverley, Robert, David Webb, Stuart Middlemiss, Phillip Duke, Anthony Clare, Keiju Okano, Colin Harwood, and Nick Aldred. "Correction to: In Vitro Oxidative Crosslinking of Recombinant Barnacle Cyprid Cement Gland Proteins." Marine Biotechnology 24, no. 2 (March 26, 2022): 430. http://dx.doi.org/10.1007/s10126-022-10107-1.

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Jiang, Z., S. Ping, C. Jin, C. Tu, and X. Zhou. "Transcriptome analysis provides insights into a molecular mechanism of histamine response in the cyprid larvae of Amphibalanus amphitrite." Marine Ecology Progress Series 681 (January 6, 2022): 1–12. http://dx.doi.org/10.3354/meps13919.

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Barnacles are notorious marine fouling creatures; their planktonic cyprid larvae attach to material substrates and metamorphose. Histamine has shown great importance in regulating cyprid settlement and metamorphosis. This study aimed to investigate the mechanisms of histamine-induced larval settlement. Cyprids were exposed to histamine or loratadine, an anti-histamine compound. The percent larval settlements of the histamine- and anti-histamine-treated cyprids were significantly higher and lower, respectively, than the control group. Transcriptomic analyses showed that histamine-treated cyprids had 18498 differentially expressed genes (DEGs, 14531 up-regulated, 3967 down-regulated) and the anti-histamine group had 18055 DEGs (17237 up-regulated, 818 down-regulated) in comparison to untreated controls. In both treatment groups, significant enrichment of DEGs involved in the mitogen-activated protein kinase signaling pathway was observed. Based on the results of larval settlement bioassays, we set 4 filter conditions to perform DEG analyses, and 19 DEGs were selected as functional genes related to cyprid settlement. The functional categories of these genes included structural proteins, spider silk proteins, energy metabolism proteins, cement proteins, glycosyl proteins, and multifunctional proteins. The energy metabolism protein AdipoR was significantly up-regulated in the histamine-treated cyprids but significantly down-regulated in the anti-histamine group. The activity of adenosine monophosphate-activated protein kinase, a downstream signaling protein of AdipoR, increased in the histamine-treated group and decreased in the anti-histamine-treated group. Our results provide new insights into the molecular mechanisms underlying the histamine-induced settlement of barnacle cyprids and identify AdipoR as an important gene that can affect the settlement of cyprids, likely through regulating cyprid energy metabolism.

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Odling, K. "An in vivo study of exocytosis of cement proteins from barnacle Balanus improvisus (D.) cyprid larva." Journal of Experimental Biology 209, no. 5 (March 1, 2006): 956–64. http://dx.doi.org/10.1242/jeb.02031.

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Perina, A., B. M. von Reumont, A. Martínez- Lage, and A. M. González-Tizón. "Accessing transcriptomic data for ecologically important genes in the goose barnacle (Pollicipes pollicipes), with particular focus on cement proteins." Marine Genomics 15 (June 2014): 9–11. http://dx.doi.org/10.1016/j.margen.2014.02.003.

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Lin, Hsiu-Chin, Yue Him Wong, Ling Ming Tsang, Ka Hou Chu, Pei-Yuan Qian, and Benny K. K. Chan. "First study on gene expression of cement proteins and potential adhesion-related genes of a membranous-based barnacle as revealed from Next-Generation Sequencing technology." Biofouling 30, no. 2 (December 12, 2013): 169–81. http://dx.doi.org/10.1080/08927014.2013.853051.

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Mohanram, Harini, Akshita Kumar, Chandra S. Verma, Konstantin Pervushin, and Ali Miserez. "Three-dimensional structure of Megabalanus rosa Cement Protein 20 revealed by multi-dimensional NMR and molecular dynamics simulations." Philosophical Transactions of the Royal Society B: Biological Sciences 374, no. 1784 (September 9, 2019): 20190198. http://dx.doi.org/10.1098/rstb.2019.0198.

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Barnacles employ a protein-based cement to firmly attach to immersed substrates. The cement proteins (CPs) have previously been identified and sequenced. However, the molecular mechanisms of adhesion are not well understood, in particular, because the three-dimensional molecular structure of CPs remained unknown to date. Here, we conducted multi-dimensional nuclear magnetic resonance (NMR) studies and molecular dynamics (MD) simulations of recombinant Megabalanus rosa Cement Protein 20 ( r MrCP20). Our NMR results show that r MrCP20 contains three main folded domain regions intervened by two dynamic loops, resulting in multiple protein conformations that exist in equilibrium. We found that 12 out of 32 Cys in the sequence engage in disulfide bonds that stabilize the β -sheet domains owing to their placement at the extremities of β -strands. Another feature unveiled by NMR is the location of basic residues in turn regions that are exposed to the solvent, playing an important role for intermolecular contact with negatively charged surfaces. MD simulations highlight a highly stable and conserved β -motif ( β 7- β 8), which may function as nuclei for amyloid-like nanofibrils previously observed in the cured adhesive cement. To the best of our knowledge, this is the first report describing the tertiary structure of an extracellular biological adhesive protein at the molecular level. This article is part of the theme issue ‘Transdisciplinary approaches to the study of adhesion and adhesives in biological systems’.

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Wong, Yue Him, Niklas Dreyer, HaoCheng Liu, Yi Lan, Jamie J. Chen, Jin Sun, Wei‐Peng Zhang, Pei‐Yuan Qian, and Benny K. K. Chan. "Gene co‐option, duplication and divergence of cement proteins underpin the evolution of bioadhesives across barnacle life histories." Molecular Ecology, August 16, 2023. http://dx.doi.org/10.1111/mec.17084.

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AbstractAcquisition of new genes often results in the emergence of novel functions and is a key step in lineage‐specific adaptation. As a group of sessile crustaceans, barnacles establish permanent attachment through initial cement secretion at the larval phase followed by continuous cement secretion in juveniles and adults. However, the origins and evolution of barnacle larval and adult cement proteins remain poorly understood. By performing microdissection of larval cement glands, transcriptome and shotgun proteomics and immunohistochemistry validation, we identified 30 larval and 27 adult cement proteins of the epibiotic turtle barnacle Chelonibia testudinaria, of which the majority are stage‐ and barnacle‐specific. While only two proteins, SIPC and CP100K, were expressed in both larvae and adults, detection of protease inhibitors and the cross‐linking enzyme lysyl oxidase paralogs in larvae and adult cement. Other barnacle‐specific cement proteins such as CP100k and CP52k likely share a common origin dating back at least to the divergence of Rhizocephala and Thoracica. Different CP52k paralogues could be detected in larval and adult cement, suggesting stage‐specific cement proteins may arise from duplication followed by changes in expression timing of the duplicates. Interestingly, the biochemical properties of larval‐ and adult‐specific CP52k paralogues exhibited remarkable differences. We conclude that barnacle larval and adult cement systems evolved independently, and both emerged from co‐option of existing genes and de novo formation, duplication and functional divergence of lineage‐specific cement protein genes. Our findings provide important insights into the evolutionary mechanisms of bioadhesives in sessile marine invertebrates.

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Lin, Hsiu-Chin, Yue Him Wong, Chia-Hsuan Sung, and Benny Kwok Kan Chan. "Histology and transcriptomic analyses of barnacles with different base materials and habitats shed lights on the duplication and chemical diversification of barnacle cement proteins." BMC Genomics 22, no. 1 (November 1, 2021). http://dx.doi.org/10.1186/s12864-021-08049-4.

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Abstract Background Barnacles are sessile crustaceans that attach to underwater surfaces using barnacle cement proteins. Barnacles have a calcareous or chitinous membranous base, and their substratum varies from biotic (e.g. corals/sponges) to abiotic surfaces. In this study, we tested the hypothesis that the cement protein (CP) composition and chemical properties of different species vary according to the attachment substrate and/or the basal structure. We examined the histological structure of cement glands and explored the variations in cement protein homologs of 12 barnacle species with different attachment habitats and base materials. Results Cement gland cells in the rocky shore barnacles Tetraclita japonica formosana and Amphibalanus amphitrite are eosinophilic, while others are basophilic. Transcriptome analyses recovered CP homologs from all species except the scleractinian coral barnacle Galkinia sp. A phylogenomic analysis based on sequences of CP homologs did not reflect a clear phylogenetic pattern in attachment substrates. In some species, certain CPs have a remarkable number of paralogous sequences, suggesting that major duplication events occurred in CP genes. The examined CPs across taxa show consistent bias toward particular sets of amino acid. However, the predicted isoelectric point (pI) and hydropathy are highly divergent. In some species, conserved regions are highly repetitive. Conclusions Instead of developing specific cement proteins for different attachment substrata, barnacles attached to different substrata rely on a highly duplicated cementation genetic toolkit to generate paralogous CP sequences with diverse chemical and biochemical properties. This general CP cocktail might be the key genetic feature enabling barnacles to adapt to a wide variety of substrata.

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Kamino, K. "Barnacle cement proteins: importance of disulfide bonds in their insolubility." Journal of Biological Chemistry, June 5, 2000. http://dx.doi.org/10.1074/jbc.m910363199.

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Schultzhaus, Janna N., Chenyue Wang, Shrey Patel, Madeline Smerchansky, Daniel Phillips, Chris R. Taitt, Dagmar H. Leary, et al. "Distribution of Select Cement Proteins in the Acorn Barnacle Amphibalanus amphitrite." Frontiers in Marine Science 7 (October 6, 2020). http://dx.doi.org/10.3389/fmars.2020.586281.

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Yuan, Jianbo, Xiaojun Zhang, Shihao Li, Chengzhang Liu, Yang Yu, Xiaoxi Zhang, Jianhai Xiang, and Fuhua Li. "Convergent evolution of barnacles and molluscs sheds lights in origin and diversification of calcareous shell and sessile lifestyle." Proceedings of the Royal Society B: Biological Sciences 289, no. 1982 (September 14, 2022). http://dx.doi.org/10.1098/rspb.2022.1535.

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The calcareous shell and sessile lifestyle are the representative phenotypes of many molluscs, which happen to be present in barnacles, a group of unique crustaceans. The origin of these phenotypes is unclear, but it may be embodied in the convergent genetics of such distant groups (interphylum). Herein, we perform comprehensive comparative genomics analysis in barnacles and molluscs, and reveal a genome-wide strong convergent molecular evolution between them, including coexpansion of biomineralization and organic matrix genes for shell formation, and origination of lineage-specific orphan genes for settlement. Notably, the expanded biomineralization gene encoding alkaline phosphatase evolves a novel, highly conserved motif that may trigger the origin of barnacle shell formation. Unlike molluscs, barnacles adopt novel organic matrices and cement proteins for shell formation and settlement, respectively, and their calcareous shells have potentially originated from the cuticle system of crustaceans. Therefore, our study corroborates the idea that selection pressures driving convergent evolution may strongly act in organisms inhabiting similar environments regardless of phylogenetic distance. The convergence signatures shed light on the origin of the shell and sessile lifestyle of barnacles and molluscs. In addition, notable non-convergence signatures are also present and may contribute to morphological and functional specificities.

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Lee, Won‐Kyung, Benny K. K. Chan, Jae‐Yoon Kim, Se‐Jong Ju, and Se‐Joo Kim. "Comparative genomics reveals the dynamic evolutionary history of cement protein genes of barnacles from intertidal to deep‐sea hydrothermal vents." Molecular Ecology Resources, November 13, 2023. http://dx.doi.org/10.1111/1755-0998.13895.

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AbstractThoracican barnacles are a diverse group of marine organisms for which the availability of genome assemblies is currently limited. In this study, we sequenced the genomes of two neolepadoid species (Ashinkailepas kermadecensis, Imbricaverruca yamaguchii) from hydrothermal vents, in addition to two intertidal species. Genome sizes ranged from 481 to 1054 Mb, with repetitive sequence contents of 21.2% to 50.7%. Concordance rates of orthologs and heterozygosity rates were between 82.4% and 91.7% and between 1.0% and 2.1%, respectively, indicating high genetic diversity and heterozygosity. Based on phylogenomic analyses, we revised the nomenclature of cement genes encoding cement proteins that are not homologous to any known proteins. The major cement gene, CP100A, was found in all thoracican species, including vent‐associated neolepadoids, and was hypothesised to be essential for thoracican settlement. Duplicated genes, CP100B and CP100C, were found only in balanids, suggesting potential functional redundancy or acquisition of new functions associated with the calcareous base. An ancestor of CP52 genes was duplicated dynamically among lepadids, pollicipedids with multiple copies on a single scaffold, and balanids with multiple sequential repeats of the conserved regions, but no CP52 genes were found in neolepadoids, providing insights into cement gene evolution among thoracican lineages. This study enhances our understanding of the adhesion mechanisms of thoracicans in underwater environments. The newly sequenced genomes provide opportunities for studying their evolution and ecology, shedding light on their adaptation to diverse marine environments, and contributing to our knowledge of barnacle biology with valuable genomic resources for further studies in this field.

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Wang, Zheng, Dagmar H. Leary, Jinny Liu, Robert E. Settlage, Kenan P. Fears, Stella H. North, Anahita Mostaghim, et al. "Molt-dependent transcriptomic analysis of cement proteins in the barnacle Amphibalanus amphitrite." BMC Genomics 16, no. 1 (October 24, 2015). http://dx.doi.org/10.1186/s12864-015-2076-1.

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So, Christopher R., Kenan P. Fears, Dagmar H. Leary, Jenifer M. Scancella, Zheng Wang, Jinny L. Liu, Beatriz Orihuela, Dan Rittschof, Christopher M. Spillmann, and Kathryn J. Wahl. "Sequence basis of Barnacle Cement Nanostructure is Defined by Proteins with Silk Homology." Scientific Reports 6, no. 1 (November 8, 2016). http://dx.doi.org/10.1038/srep36219.

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Fan, Hailong, Jiahui Wang, and Jian Ping Gong. "Barnacle Cement Proteins‐Inspired Tough Hydrogels with Robust, Long‐Lasting, and Repeatable Underwater Adhesion." Advanced Functional Materials, December 28, 2020, 2009334. http://dx.doi.org/10.1002/adfm.202009334.

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Liu, Yongchun, Ke Li, Juanhua Tian, Aiting Gao, Lihua Tian, Hao Su, Shuting Miao, et al. "Synthesis of robust underwater glues from common proteins via unfolding-aggregating strategy." Nature Communications 14, no. 1 (August 24, 2023). http://dx.doi.org/10.1038/s41467-023-40856-z.

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AbstractUnderwater adhesive proteins secreted by organisms greatly inspires the development of underwater glue. However, except for specific proteins such as mussel adhesive protein, barnacle cement proteins, curli protein and their related recombinant proteins, it is believed that abundant common proteins cannot be converted into underwater glue. Here, we demonstrate that unfolded common proteins exhibit high affinity to surfaces and strong internal cohesion via amyloid-like aggregation in water. Using bovine serum albumin (BSA) as a model protein, we obtain a stable unfolded protein by cleaving the disulfide bonds and maintaining the unfolded state by means of stabilizing agents such as trifluoroethanol (TFE) and urea. The diffusion of stabilizing agents into water exposes the hydrophobic residues of an unfolded protein and initiates aggregation of the unfolded protein into a solid block. A robust and stable underwater glue can thus be prepared from tens of common proteins. This strategy deciphers a general code in common proteins to construct robust underwater glue from abundant biomass.

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Pan, Gaoxing, Feihan Li, Shaohua He, Weidong Li, Quanming Wu, Jingjing He, Renjie Ruan, Zhixiang Xiao, Jin Zhang, and Huanghao Yang. "Mussel‐ and Barnacle Cement Proteins‐Inspired Dual‐Bionic Bioadhesive with Repeatable Wet‐Tissue Adhesion, Multimodal Self‐Healing, and Antibacterial Capability for Nonpressing Hemostasis and Promoted Wound Healing." Advanced Functional Materials, March 18, 2022, 2200908. http://dx.doi.org/10.1002/adfm.202200908.

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"Enhanced bone regeneration by Aloe vera gel conjugated barnacle cement protein composite hyaluronic acid hydrogel based hydroxyapatite derived from cuttlefish bone." Medical & Clinical Research 7, no. 3 (March 14, 2022). http://dx.doi.org/10.33140/mcr.07.03.02.

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Injectable Aloe vera gel W (AGW®)-conjugated Barnacle Cement Protein (BCP) composite Hyaluronic Acid (HA) hydrogels provide local periodontal tissue for bone filling in periodontal surgery. We developed a novel type of injectable self-supported hydrogel (2 mg/ml of AGW®-BCP/HA) based cuttlefish bone derived hydroxyapatite (CBH) for dental graft, which could good handling property, biodegradation or biocompatibility with the hydrogel disassembly and provided efficient cell adhesion activity and no inflammatory responses. Herein, the aim of this work was to evaluate bone formation following implantation of CBH and collagen membrane in rabbit calvarias defects. Eight male New Zealand rabbits were used and four circular calvarias defects were created on each animal. Defects were filled with different graft materials: 1) collagen membrane, 2) collagen membrane with CBH, 3) collagen membrane with bovine bone hydroxyapatite (BBH), and 4) control. The animals were sacrificed after 2 and 8 weeks of healing periods for histologic analysis. Both sites receiving CBH and BBH showed statistically increased augmented volume and new bone formation (p<0.05). However, there was no statistical difference in new bone formation between the CBH, BBH and collagen membrane group at all healing periods. Within the limits of this study, collagen membrane with CBH was an effective material for bone formation and space maintaining in rabbit calvarias defects.

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Assadizadeh, Mohammad, Nima Goodarz, Amir Hossein Mahdavi Pak, Seyyed Mohammad Hasan Haghayeghi, and Maryam Azimzadeh Irani. "Structural investigation of Amphibalanus amphitrite cement proteins: an in-silico study." Bioinspired, Biomimetic and Nanobiomaterials, October 28, 2023, 1–11. http://dx.doi.org/10.1680/jbibn.23.00008.

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Balanomorpha, commonly known as barnacles, are leading biofouling animals belonging to subclass Cirripedia that adhere durably to different submerged surfaces by utilizing a chiefly-proteinaceous cement. According to prior experiments, adhesion is most likely made possible by the self-assembling aggregates reputed as amyloid-like nanofibers. The secreted cement contains numerous proteins among which CP19k and CP20k are thought to have a substantial influence on the adhesion process. The molecular configuration and atomistic interactions that result in this firm cement are not yet completely understood. Herein, AI-based structure prediction and molecular dockings were used to inspect the potential role of AaCP19k and AaCP20k-1 of Amphibalanus amphitrite in the formation of amyloid-like nanofibers. The anticipated structure of AaCP19k was highly accurate and its β-sandwich folding had a close resemblance to cross-β motifs found in amyloid nanofibers. In the AaCP19k, β1-2 and β7-8 act as oligomerization sites where stable dimers and trimers can be assembled. These modeled oligomerization interfaces point to the self-assembly site through which fibrillization might happen. The structural flexibility of AaCP20k-1 yielded low-accurate models, but a conserved β-hairpin and an α-helix were evident with high confidence. These structural properties can be employed in prospective studies to develop bioadhesives and design anti-fouling substances.

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Li, Baoshan, Junyi Song, Ting Mao, Ling Zeng, Zonghuang Ye, and Biru Hu. "An essential role of disulfide bonds for the hierarchical self-assembly and underwater affinity of CP20-derived peptides." Frontiers in Bioengineering and Biotechnology 10 (October 12, 2022). http://dx.doi.org/10.3389/fbioe.2022.998194.

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Abstract:

Barnacles are typical fouling organisms strongly adhere to immersed solid substrates by secreting proteinaceous adhesives called cement proteins (CPs). The self-assembly of the CPs forms a permanently bonded layer that binds barnacles to foreign surfaces. However, it is difficult to determine their natural structure and describe their self-assembly properties due to the abundance of cysteines in whole-length CP20. A putative functional motif of Balanus albicostatus CP20 (BalCP20) was identified to present distinctive self-assembly and wet-binding characteristics. Atomic-force microscopy (AFM) and transmission electron microscope (TEM) investigations showed that wildtype BalCP20-P3 formed grain-like spindles, which assembled into fractal-like structures like ears of wheat. SDS-PAGE, AFM, and LSCM showed that DTT treatment opened up disulfide bonds between cysteines and disrupted fractal-like structures. Additionally, these morphologies were abolished when one of the BalCP20-P3 four cysteines was mutated by alanine. Circular dichroism (CD) results suggested that the morphological diversity among BalCP20-P3 and its mutations was related to the proportion of α-helices. Finally, quartz crystal microbalance with dissipation (QCM-D) detected that BalCP20-P3 and its mutations with diverse self-assemblies occupied different affinities. The above results demonstrated that cysteines and disulfide bonds played a crucial role in the self-assembly and wet binding of BalCP20-P3. The work provides new ideas for the underwater bonding of BalCP20 and developing new bionic underwater adhesives.

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Journal articles on the topic 'Barnacle cement proteins'

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