Academic literature on the topic 'Balinese Primers'

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Journal articles on the topic "Balinese Primers"

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Ariati, P. E. P., I. G. P. Wirawan, and M. M. V. Sasadara. "Optimization of primer and polymerase chain reaction conditions to amplify COI locus for identification of Purnajiwa (Euchresta horsfieldii (Lesch.) Benn.) collected from Bedugul, Bali." IOP Conference Series: Earth and Environmental Science 913, no. 1 (November 1, 2021): 012069. http://dx.doi.org/10.1088/1755-1315/913/1/012069.

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Abstract The polymerase chain reaction (PCR) has been used for molecular research to amplify DNA fragments, especially from a small amount of genetic material. PCR has been applied for numerous researches, including for plant molecular identification. A good PCR product is dependent on good amplification of the DNA segment in optimum condition. This research was conducted to optimize the primer combination, and the annealing temperature for DNA barcoding application of Balinese rare medicinal plant (Euchresta horsfieldii (Lesch.) Benn.) collected from Bedugul. COI is a suitable primer to identify unknown species to higher taxa levels and species with high phenotypic plasticity. The range of annealing temperatures was tested to amplify the combination of five COI primers: GWSF and GWSF5 for forwarding primers and GWSR, GWSR3, and GWSR5 for reverse primers. The annealing temperature was 52, 54, 56, 58, 60°C for 30 seconds. The result showed that GWSF-GWSR, GWSF-GWSR5, GWSF5-GWSR, GWSF5-GWSR5, and GWSF5-GWSR3 produced multiple bands, the double band that cannot be cut, multiple bands, faint amplification band for GWSF5-GWSR5 and GWSF5-GWSR3, respectively. Primer combination of GWSF-GWSR3 with an annealing temperature of 52°Cproduced double bands that were available to have proceeded for sequencing. In conclusion, the combination of GWSF-GWSR3 that is annealed at 52°C produced the best amplification band. The primer combinations and conditions can be used for further identification of Purnajiwa collected from Bedugul.
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Putu Eka Pasmidi Ariati, Maria Malida Vernandes Sasadara, I Gede Putu Wirawan, Made Sritamin, I Ketut Suada, I Nyoman Wijaya, Rindang Dwiyani, I Putu Sudiarta, and Ida Ayu Putri Darmawati. "Application of DNA Barcoding for authentication of Balinese traditional medicinal plant Purnajiwa (Kopsia arborea Blume. and Euchresta horsfieldii) (Lesch.) Benn." Bali Medical Journal 11, no. 3 (November 21, 2022): 1681–85. http://dx.doi.org/10.15562/bmj.v11i3.3815.

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Background: Purnajiwa is one of the medicinal plants in Bali. Species information regarding this plant is still unconfirmed. Currently, this plant is considered rare and endangered. Molecular identification supports authentication at the species level, which will also support conservation efforts. Method: Molecular identification using DNA Barcoding was carried out on purnajiwa from three sampling locations in Bali (Jimbaran, Mambal, and Bedugul) using rbcL primers (rbcLaF and rbcLaR). Data analysis was performed using BLAST with species on GenBank. Pairwise and similarity values ​​were used to measure the three samples' proximity to the Gen Bank species. Result: The phylogenetic tree was constructed with the Maximum Likelihood and Tamura-3-parameter model. The analysis results show a close relationship between the purnajiwa from Jimbaran and Mambal with the species Kopsia arborea (KP095079). Meanwhile, the Purnajiwa from Bedugul showed a difference from the other two purnajiwa and was closely related to Euchresta horsfieldii (U74225). Conclusion: It can be concluded that the Purnajiwa from Jimbaran and Mambal is a species of Kopsia arborea while the Purnajiwa from Bedugul is a species of Euchresta horsfieldii.
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Sukmawati, Dalia, Mutia Balqis, Adisyahputra Adisyahputra, Muktiningsih Nurjayadi, Sheyla Annisyah, Famira Ichsanty, Atin Supiyani, et al. "The Potential of Cellulolytic Yeast Pichia manshurica UNJCC Y-123, Saccharomyces cerevisiae UNCC Y-84, and Saccharomyces cerevisiae UNJCC Y-83 to Produce Cellulase Enzyme by Using Substrate Skin Delignification of Cocoa (Theobroma cocoa)." Trends in Sciences 20, no. 10 (July 31, 2023): 6950. http://dx.doi.org/10.48048/tis.2023.6950.

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Exploring the possibility of using agricultural waste as a substrate for the synthesis of cellulase enzymes for fuel and renewable energy is the main goal of this study, which is in line with the notions of sustainable development and environmental stewardship. The product of delignification of cocoa peel can be used as a substrate for cellulase enzymes producing by yeast isolated from Balinese palm wine. Cellulase enzymes made from yeast from Balinese palm wine can be produced using the delignified cocoa peel as a substrate. This study aims to analyze the cellulase enzyme activity of yeast from Bali's palm wine on delignified cocoa bark substrates. The tests carried out were delignification of cocoa bark (solvent variations: HCl, H2SO4 and NaOH; concentrations: 1, 1.5 and 2 %; biomass 1:15 and 1:20 (w/v)); screening of cellulolytic yeast from Balinese palm wine; molecular identification and morphological characteristics of yeast; and cellulase enzyme activity (variation of yeast isolates and fermentation time of 48, 72 and 96 h). The results of delignification of cocoa shells showed that the use of 1.5 % NaOH solvent with cocoa shell powder biomass of 1:15 (9.59 ± 0.11) significantly differed from the value of reducing sugar content. Based on the screening results of 6 yeast isolates, 3 yeast isolates with the highest cellulolytic index values were selected, namely UNJCC Y-83 (0.29 ± 0.01 mm), UNJCC Y-123 (0.24 ± 0.01 mm) and UNJCC Y-84 (0.23 ± 0.01 mm). For testing cellulase enzyme activity. S. cerevisiae UNJCC Y-83 (4.11 ± 0.41 U/mL); S. cerevisiae UNJCC Y-84 (4.11 ± 0.33 U/mL) and P. manshurica UNJCC Y-123 (4.06 ± 0.12 U/mL) at 96 h of fermentation had significantly different cellulase enzyme activity. The results of identification of yeast rDNA in the D1/D2 region with NL1/NL4 primers obtained the identity of Saccharomyces cerevisiae UNJCC Y-83; Saccharomyces cerevisiae UNJCC Y-84 (99.66 % homology); and Pichia manshurica (100 % homology). Theobroma cocoa rind can be used as a yeast substrate to produce cellulase enzymes for fuel and renewable energy. HIGHLIGHTS Screening of 6 yeast isolates, 3 yeast isolates with the highest cellulolytic index values were selected, namely UNJCC Y-83 (0.29 ± 0.01 mm), UNJCC Y-123 (0.24 ± 0.01 mm) and UNJCC Y-84 (0.23 ± 0.01 mm) for testing molecular identification and cellulase enzyme activity The result of rDNA sequence analysis in the D1/D2 region showed that the yeast isolates UNJCC Y-83 and UNJCC Y-84 had a homology level of 99.66 % as Saccharomyces cerevisiae with a bootstrap value of 92 %. UNJCC Y-123 yeast isolate has a homology level of 100 % as Pichia manshurica with a bootstrap value of 100 % cerevisiae UNJCC Y-83 (4.11 ± 0.41 U/mL); S. cerevisiae UNJCC Y-84 (4.11 ± 0.33 U/mL); and P. manshurica UNJCC Y-123 (4.06 ± 0.12 U/mL) at 96 h of fermentation had significantly different cellulase enzyme activity Theobroma cocoa rind can be used as a yeast substrate to produce cellulase enzymes for fuel and renewable energy GRAPHICAL ABSTRACT
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Selangga, Dewa Gede Wiryangga, and Listihani Listihani. "Squash Leaf Curl Virus: Species of Begomovirus as the Cause of Butternut Squash Yield Losses in Indonesia." HAYATI Journal of Biosciences 29, no. 6 (July 7, 2022): 806–13. http://dx.doi.org/10.4308/hjb.29.6.806-813.

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Curling symptom was found in pumpkin plants in Bali, and the PCR detection result using Begomovirus universal primers indicated Begomovirus infection. Further research was conducted to determine the distribution, molecular character of SLCV, and yield loss of the pumpkins. Sampling was carried out at pumpkin plantations in 9 districts in Bali. Detection and identification were carried out using PCR method, followed by cloning and DNA sequencing. DNA band with the measurement of 900 bp was successfully amplified from several pumpkin samples from Denpasar, Gianyar, and Buleleng. Homology analysis of nucleotide sequences using the database in GenBank of SLCV of Balinese isolates showed the highest homology and kinship of 97.3-98.4% and 98.4-99.3% respectively with East Timor isolates from pumpkin plants. The phylogeny analysis showed that SLCV Indonesian isolates were in the same group as Asian isolates. The result of this study is the first report on infection and molecular characterization of SLCV in pumpkin plants in Indonesia. The yield loss caused by curling disease on individual pumpkin plants was 56.3%, and the disease caused a decrease in the quality of harvest fruits. Yield loss estimation caused by the disease in fields with different levels of disease intensity ranged from 10.02 to 25.83%. It was equal to yield loss ranging from IDR 878,400.00 to IDR 10,826,400.00 depending on the disease intensity. It is found that the correlation between curling disease severity and yield loss was high.
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Susiani, Ketut. "Maintenance Efforts of Bali Culture and Language Through Local Content in Primary Schools." Bisma The Journal of Counseling 5, no. 1 (May 30, 2021): 9. http://dx.doi.org/10.23887/bisma.v5i1.34228.

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This study uses a qualitative case study approach, which is a study that aims to describe a condition that is happening in the real world. The research procedure adopted the case study research procedure by Yin which includes (1) design the case study protocol; (2) Conduct the case study; (3) Analyze case study evidence: and (4) Develop conclusions, recommendations, and implications based on the evidence. The subjects of this study were elementary school teachers and students, and school documents were examined related to the maintenance of Balinese culture and language through local content carried out in schools. The data collection technique was carried out by structured interviews using interview guidelines related to maintaining Balinese language culture and Balinese local content. Observations were made with a participatory observation approach, and documentation studies were carried out by collecting documents related to the maintenance of Balinese culture and Balinese language in schools. Qualitative data analysis techniques began with grouping data based on primary data sources and secondary data. The results of this study indicate that the application of local content in elementary schools is one of the contents that can be used in maintaining, preserving and preserving the Balinese language culture in schools. The results of surveys, observations and interviews at SD Buleleng indicate that the maintenance of Balinese culture and language has many weaknesses, seen from the use of local languages that are not used in communication and Balinese culture which is fading due to the influence of other cultures. This can be seen in the language used in schools not using Balinese in daily communication in the sense that many do not understand the meaning of the Balinese language used because SOR is Singgih Bahasa. The results of study have and important implication to the teaching learning contents of Balinese language in elementary school in Bali, Indoensia.
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Arnawa, Nengah. "The Implementation of Natural Semantic Metalanuage and Semantic Field in Language Teaching: A Case Study." Journal of Language Teaching and Research 8, no. 3 (May 2, 2017): 507. http://dx.doi.org/10.17507/jltr.0803.08.

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This study aimed at developing a model of learning Balinese vocabularies by implementing Natural Semantic Meta-language (NSM) and semantics field theory. This finding was expected to establish a natural atmosphere of Balinese learning based on semantics primes. Semantics primes development on the basis of semantic field can overcome abstractness meaning of certain words. To prove the truth of the theory construction, the researcher modified a research and development which refers to Borg and Gell models. Data were collected before and after the development of model with certain indicators: the rapid in understanding words meaning, skill in using the word in natural sentence structure, and students’ learning creativity. Significance was measured by using t-test formula for correlated samples. Based on statistical analysis, it was proved that the implementation of NSM and semantic field theory was very effective (significant) in learning Balinese vocabularies on students grade 1, 2, and 3. Effectiveness of learning was achieved because the materials adhering to the natural principle of language acquisition and the availability of direct meaning comparison between words were intuitively related. Direct comparison could overcome the cognitive limitations of students grade 1, 2, and 3 in primary school.
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Swandana, I. Wayan, Ketut Artawa, I. Nyoman Udayana, and Ketut Widya Purnawati. "The Alignment of Verb Ada and Its Existence to Understand the Existential Clause Structure of Balinese." Journal of Language Teaching and Research 15, no. 4 (July 1, 2024): 1144–55. http://dx.doi.org/10.17507/jltr.1504.12.

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An existential clause is a clause that shows a proposition about the existence or an event. This paper aims to describe the structure of existential clauses, the types of existential clauses, and the constraints of noun phrases that fill the existence elements in Balinese. This study used the descriptive qualitative method. The data used are primary data taken from five Balinese language teachers. The Balinese language teachers elicited the data made by the first teacher. Written data from Balinese stories are also used to assist the oral data. The data analysis technique chosen is comparison, omission, and replacement. Some research results are found in this paper. The core structure of Balinese existential clauses is verb ada + existence, and the extended structure of Balinese existential clauses is verb ada + existence + extension. There are two types of existential clauses in Balinese: bare and extended existential clauses. The definiteness of a noun phrase filling the existence element determines the choice of existential or non-existential clauses. This paper is helpful as a reference for Balinese grammar, which concerns the existential clauses of Balinese.
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Sanjaya, Dewa Bagus, I. Kadek Suartama, I. Nengah Suastika, Sukadi Sukadi, and I. Putu Mas Dewantara. "The implementation of balinese folflore-based civic education for strengthening character education." Cypriot Journal of Educational Sciences 16, no. 1 (February 25, 2021): 303–16. http://dx.doi.org/10.18844/cjes.v16i1.5529.

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The objective of this study is to analyse the affectivity of satua (Balinese folklore)-based character education in the instructions of civic education in primary schools in Buleleng Regency, Bali. This study used the experimental design in testing the affectivity of folklore-based instructions in the competency and character of primary school students. The instruments used to collect data in this study were questionnaires, test of learning outcomes, score inventory and self-evaluation. The questionnaire was designed to find out the feasibility of folklore-based instructions. The test on learning outcomes was used to find out their scores. The data analysis used the multivariate analysis of variance. The results of the study indicated that a) learning using Balinese folklore is better than conventional learning in students’ civic knowledge, b) learning using Balinese folklore is better than conventional learning in students’ attitude and c) learning using Balinese folklore is better than conventional learning in students’ skills. Keywords: Balinese folklore, civic education, character education
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Paramarta, I. Made Suta. "Balinese Language on the Street Signs in Singaraja Town, Bali: A Linguistic Landscape Analysis." Ranah: Jurnal Kajian Bahasa 11, no. 1 (June 26, 2022): 120. http://dx.doi.org/10.26499/rnh.v11i1.4197.

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The existence of local languages in Indonesia, including Balinese language is often related to marginalization issue. Some previous research revealed that Balinese language is rarely used in public spaces. The local government implements Governor Regulation Number 80 of 2018 to officially protect the language, including its existence in public spaces. This research explores the existence of Balinese language and script on the lingual street signs in Singaraja, North Bali. Further, the distribution of the lingual street signs is also investigated to reveal the government's primary and secondary target areas in language protection. The subjects of the study are 151 street signs that are placed by the department of transportation of Buleleng regency. The data are obtained through photograph taking and observation. The research reveals that the presence of Indonesian language marginalizes Balinese language. The regulation only successfully improves the use of Balinese script instead of the use of Balinese language. In terms of the sign distribution, the government focuses on signs with Balinese script at the center of the town due to the intense language contact in the area. AbstrakKeberadaan bahasa daerah di Indonesia, termasuk bahasa Bali sering dihubungkan dengan isu marginalisasi. Beberapa penelitian sebelumnya mengungkapkan bahwa bahasa Bali jarang digunakan di ruang publik. Pemerintah daerah Bali telah menerapkan Peraturan Gubernur Nomor 80 Tahun 2018 untuk secara resmi melindungi bahasa dan aksara Bali, termasuk keberadaannya di ruang publik. Sehubungan dengan itu, penelitian ini menggali keberadaan bahasa dan aksara Bali pada rambu-rambu jalan lingual di Singaraja, Bali Utara. Selanjutnya, sebaran rambu-rambu jalan lingual juga diselidiki untuk mengungkap wilayah sasaran primer dan sekunder pemerintah dalam perlindungan bahasa Bali. Subyek penelitian adalah 151 rambu jalan yang dipasang oleh Dinas Perhubungan Kabupaten Buleleng. Data diperoleh melalui pengambilan foto dan observasi. Hasil penelitian mengungkapkan bahwa bahasa Bali terpinggirkan dengan kehadiran bahasa Indonesia. Peraturan gubernur tersebut hanya berhasil meningkatkan penggunaan aksara Bali ketimbang penggunaan bahasa Bali. Dalam hal distribusi tanda, pemerintah memfokuskan penggunaan tanda dengan aksara Bali di pusat kota karena kontak bahasa yang intens terjadi di daerah tersebut.
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Padmayanti, Ni Luh Putu, and A. A. Istri Ngurah Marhaeni. "Factors Influencing Bali Women's Decision to Enter The Labor Market in West Denpasar Sub-District." Journal of Comprehensive Science (JCS) 3, no. 6 (June 27, 2024): 1126–39. http://dx.doi.org/10.59188/jcs.v3i6.762.

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This study aims to analyze the simultaneously and partially effect of age, reconciliation of gender roles, work ethic of Balinese women, husband's income and education on the decision of Balinese women to enter the labor market in West Denpasar District. Analyze the role of education in moderating effect of husband's income and to find out the variables that have a dominant effect on the decision of Balinese women to enter the job market in West Denpasar District. This research design uses quantitative methods with a total sample of 100 married women. This study uses primary data collected through observation, structured interviews and indepth interviews. Data analysis techniques were carried out by logistic regression analysis, moderation, and dominant test. The results showed that age, gender role reconciliation, Balinese women's work ethic, husband's income and education simultaneously had a significant effect on the chances of Balinese women's deciding to enter the labor market. Partially, age, reconciliation of gender roles, Balinese women's work ethic, and education have a positive and significant effect on the chances of Balinese women's deciding to enter the labor market while, husband's income has no negative or insignificant effect of Balinese women's deciding to enter the labor market. The level of education as a moderating variable weakens the effect of husband's income on the chances of Balinese women's deciding to enter the labor market. Education has a dominant effect on the chances of Balinese women's deciding to enter the labor market.
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Book chapters on the topic "Balinese Primers"

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Bruner, Jerome. "Dreamtime Learning, Outside-In: Murngin Age-Grading Rites." In Culture In Mind, 236–62. Oxford University PressNew York, NY, 1996. http://dx.doi.org/10.1093/oso/9780195095975.003.0011.

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Abstract The case for culture in mind rests on an understanding of how public models—what I call instituted models—are internalized by members of a community and born again as mental models—the cultural dimensions of personal experience. The study of cultural learning was pioneered by Margaret Mead and Gregory Bateson. In New Guinea and in Bali, they used photography to document the cultural messages embedded in the culturally patterned behavior between caretakers and children. This “cultural communication” was assumed to shape the children’s perception of fundamental aspects of experience (Bateson and Mead, 1942). Mead and Bateson studied what they called “Balinese character” outside-in. Their primary data were not psychometric tests, life histories, or psychodynamic interviews but rather photos of a large number of standardized behavioral patterns that were assumed to model for children basic orientations to the world.
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Conference papers on the topic "Balinese Primers"

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Riastini, Putu, Asri Widowati, Yuni Ratnasari, and IGP Darma. "Enganging Balinese Culture and Technology in Digital Era: A Review to Foster Primary Teachers’ Competencies." In Proceedings of the 6th International Conference on Educational Research and Innovation (ICERI 2018). Paris, France: Atlantis Press, 2019. http://dx.doi.org/10.2991/iceri-18.2019.50.

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