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Dissertations / Theses on the topic 'Bakteriom'

Author: Grafiati

Published: 28 June 2021

Last updated: 3 February 2022

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1

Hofherr, Linda [Verfasser]. "Adhäsion des produktiven gramnegativen Bakteriums Paracoccus seriniphilus sowie grampositiver Bakterien an Bauteiloberflächen / Linda Hofherr." München : Verlag Dr. Hut, 2019. http://d-nb.info/1200755022/34.

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2

Pernicová, Iva. "Identifikace a izolace PHA produkujících bakterií." Doctoral thesis, Vysoké učení technické v Brně. Fakulta chemická, 2021. http://www.nusl.cz/ntk/nusl-438296.

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Polyhydroxyalkanoates (PHA) are microbial storage polyesters that represent a renewable and environmentally friendly alternative to petrochemical plastics. However, their production and use are severely disadvantaged by the high production cost. The use of extremophilic PHA producers is one of the ways to reduce the cost of PHA production. Extremophiles bring numerous advantages resulting from the high robustness of the process against microbial contamination. In this doctoral thesis, attention was focused on the study of PHA production using selected halophilic and thermophilic microorganisms. Representatives of the genus Halomonas were mainly from public collections of microorganisms. Two promising PHA producers on waste frying oil were identified, namely Halomonas hydrothermalis and Halomonas neptunia. Both strains achieved good PHA yields in flask experiments. With the addition of suitable structural precursors, they were also able to produce copolymers with interesting material properties. However, in the proposed thesis, the main emphasis was placed on the study of PHA production using thermophilic microorganisms. As a part of the work, the isolation of thermophilic PHA producers from various thermophilic consortia (active sludge, compost, etc.) was performed. During isolations experiments, an original isolation procedure was designed using changes in osmotic pressure, the so-called osmoselection. Dozens of promising thermophilic PHA producers were obtained thanks to this original approach. They were taxonomically classified using 16S rRNA and tested for production potential. The most promising PHA producer was the isolate which was classified as Aneurinibacillus sp. H1. This bacterium is able to utilize a variety of substrates, including waste glycerol, to produce PHA. Even more important is the capability of synthesizing copolymers with a high content of 4-hydroxybutyrate. The monomer composition of the PHA copolymer and thus the material properties of the prepared copolymer can be controlled by suitable adjustment of the cultivation conditions. The prepared copolymer P(3HB-co-4HB) has unique properties and the great application potential in numerous high-end applications, for example in the field of health care, food industry or cosmetics.

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3

Wenning, Mareike, Siegfried Scherer, and Henriette Mietke-Hofmann. "Differenzierung probiotischer Bakterien." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2011. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-76077.

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Die Fourier-Transform-Infrarot-(FTIR)-Spektroskopie ist prinzipiell in der Lage, Mikroorganismen auf Stammesebene zu trennen und zu differenzieren. Das Projekt hat zum Inhalt, bestehende FT-IR-Datenbanken um handelsübliche probiotische Futterzusatzstoffe zu erweitern. Nach der erfolgreichen Erarbeitung einer Datenbank zur sicheren Differenzierung von ubiquitären und probiotischen B. cereus-Stämmen wurden die Datenbanken um probiotische Sporenbildner der Spezies B. subtilis und B. licheniformis sowie um probiotische Milchsäurebakterien (Enterococcus, Lactobacillus, Pediococcus) erweitert. Alle probiotischen Stämme lassen sich sicher von ubiquitär vorkommenden Mikrooganismen der gleichen Spezies trennen, zumeist gelingt auch die Differenzierung der Probiotika untereinander. Diese einfache, preiswerte, schnelle und dennoch exakte Methode stellt einen enormen Fortschritt für die Futtermittelmikrobiologie dar.

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4

Schräder, Thomas. "Biotransformation und Abbau heterozyklischer Verbindungen durch Bakterien." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=964716046.

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5

Panjikar, Santosh. "Crystallographic studies of bacterial single stranded DNA-binding proteins." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=964154366.

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6

Kalippke, Kai. "Interaktionen endophytischer Bakterien mit ihren Wirtspflanzen auf zellulärer Ebene und deren Einfluss auf die Phytopathogenantwort." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=960294090.

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7

Henze, Björn. "Nachweissysteme zur Genexpressionsanalytik von Bakterien." [S.l. : s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=967588464.

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8

Schiefner, André. "X-ray crystallographic studies on bacterial proteins involved in active membrane transport MalFGK2, MalE, ProX, and AcrB /." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972346732.

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9

Witte, Gregor. "Bakterielle Einzelstrang-DNA-bindende Proteine und ihre Wechselwirkungen." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=973166282.

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10

Scheyhing, Carsten Heiko. "Hochdruckinduzierte Genexpression bei Bakterien." [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=967121825.

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11

Medina, Rodríguez Luisa Marcela. "Floc formation in wastewater treatment systems using algal bacterial symbiosis /." Hamburg : Techn. Univ. Hamburg-Harburg, 2006. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=015584416&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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12

Oberreuter, Helene. "FT-IR spectroscopic identification and infraspecific diversity of coryneform bacteria in relation to 16S rDNA sequence analysis." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=962146374.

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13

Daims, Holger. "Population structure and functional analyses by in situ techniques of nitrifying bacteria in wastewater treatment plants." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963771582.

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14

Wettstein, Felipe. "Anreicherung, Isolierung und taxonomische Zuordnung ungewöhnlicher Chloraromaten-Verwerter und ihre Nutzung zum Test von PCR-Primern für den Nachweis von Genen des Chlorbrenzcatechin-Weges." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=974004014.

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15

Olsson, Jan. "Delivery of type III secreted toxins by Yersinia pseudotuberculosis : the role of LcrV, YopD, and free lipids in the translocation process /." Umeå : Umeå University, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-800.

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16

Kraft, Louise. "Crystallographic studies of gluconate kinase /." Stockholm : [Karolinska institutets bibl.], 2001. http://diss.kib.ki.se/2001/91-7349-055-5/.

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17

Sahly, Hany. "Interactions of Klebsiella pneumoniae with host cells and their contribution to pathogenesis /." Aachen : Shaker, 2003. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=012927001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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18

Snigula, Heike. "(Bakterio-)Chlorophyll-Modifikationen zur Einlagerung in synthetische Peptide." Diss., lmu, 2003. http://nbn-resolving.de/urn:nbn:de:bvb:19-12496.

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19

Pape, Thomas. "Lipidbiomarker schwammassoziierter Bakterien und Archaeen." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=97153621X.

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20

Keil, Claudia. "Physiologie nährstofflimitierter Bakterien in Membranbioreaktoren." Clausthal-Zellerfeld Papierflieger, 2007. http://d-nb.info/985706538/04.

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21

Briese, Olaf. "Über kulturelle Ursprünge des Bakteriums." Berlin : Akad.-Verl, 2003. http://hsozkult.geschichte.hu-berlin.de/rezensionen/2003-4-089.

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22

Kolinko, Sebastian. "Einzelzell-Analyse unkultivierter magnetotaktischer Bakterien." Diss., Ludwig-Maximilians-Universität München, 2014. http://nbn-resolving.de/urn:nbn:de:bvb:19-178501.

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23

Fischerová, Lenka. "Studium biodegradace syrovátky termofilními bakteriemi." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216419.

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This thesis deals with the possibilities of biodegradation of whey by the means of a mixed thermophilic aerobic bacterial culture of the Bacillus and Thermus genera. After protein had been removed, the whey was used as a medium for cultivation of the thermophilic microorganisms. The cultivations took their course in a fermentation unit and in the Erlenmayer flasks in a heated shaker in a whey medium. The bioengineering characteristics of the cultivation processes and the degree of biodegradation of the whey were evaluated. Scale of the whey biodegration was judged through the analytical characteristics – concentration of biomass, laktose and a chemical oxygen demand (COD). A decrease of CHSK was detected in all cultivations. Maximal reduction of CHSK was happend always in c. first half of each exponential phase growt, i.e. about 15 ± 3 % after first growth phase and sumarily about 62 ± 4 % after second growth phase.

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24

Kazemi, Nahid. "Bakteriemi vid tandvård- En litteraturstudie." Thesis, Malmö högskola, Odontologiska fakulteten (OD), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19909.

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Syftet: Syftet med denna studie var att kartlägga magnitud och incidens av bakteriemi vid tandbehandling, vid egen munvård samt vid tuggning.Material och metod: En systematisk sökning i PubMed och Cochrane Library genomfördes. Den systematiska sökningen resulterade i 1113 artiklar. Titlar och abstrakt granskades med hjälp av tre oberoende bedömare. Totalt 90 artiklar lästes i fulltext. Granskningen av artiklar i fulltext genomfördes med hjälp av förutbestämda inklusions- och exklusionskriterier. Data extraherades ur utvalda artiklar och sammanställdes i en tabell.Resultat: Totalt inkluderades 42 artiklar. Resultaten visade hög incidens av bakteriemi vid tandextraktion och låg incidens vid avtryck, fastsättning/avlägsnande av ortodontiska band och separationsligatur, låg- och högvarvspreparation, samt suturtagning. Bland egna munaktiviteter hade tuggning och rengöring med tandpetare låg incidens medan tandborstning och rengöring med tandtråd hade högre incidens. Endast 16 artiklar redovisade magnitud och den redovisades med så låg noggrannhet att resultaten inte bedömdes användbara för att dra slutsatser.Slutsatser: Alla dentala ingrepp ingående i studiens sökning kan leda till bakteriemi av varierande grad. Egen munvård och tuggning kan leda till bakteriemi. Egen munvård och tuggning medför mindre risk för uppkomst av bakteriemi jämfört med de dentala åtgärder (tandextraktion, subgingival depuration och dentoalveolär kirurgi), där antibiotikaprofylax rekommenderas enligt Läkemedelsverket.
Aim: The purpose of this study was to identify the magnitude and incidence of bacteremia in dental procedures, at own routine mouth activities and at chewing.Materials and methods: A systematic search in PubMed and Cochrane Library database was done. This resulted in 1113 articles. Titles and abstracts were reviewed by three independent assessors. A total of 90 articles were read in full text applying the inclusion and exclusion criteria. Data were extracted from included articles. Results of bacteremia incidence were charted in a table.Results: Forty-two articles were included. The result showed a high incidence of bacteremia at extraction and low incidence at impression, rubberdam placement, removal or attachment of orthodontic bands and separator, fast and slow drill. Routine mouth activities like chewing and cleaning by toothpicks have low incidence while tooth brushing and flossing have higher incidence. Only 16 articles reported magnitude and it reported with as low accuracy that the results could not be used to draw any conclusion.Conclusions: All dental procedures that were found at the search concerning this study can lead to bacteremia in varying degree. Own routine mouth activities and chewing can lead to bacteremia. Routine daily mouth activities have less risk for the appearance of bacteremia compared with the dental measures (tooth extraction, subgingival scaling and dentoalveolar surgery) when antibiotic prophylaxis is recommended according to recommendations of Läkemedelsverket.

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25

Jendretzke, Kai. "Untersuchungen zu Laktosegehalt, somatischer Zellzahl und bakteriologischer Beschaffenheit von Ziegenmilch aus hessischen Beständen." Giessen VVB Laufersweiler, 2009. http://d-nb.info/996005889/04.

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26

Rabenstein, Björn. "Monte Carlo methods for simulation of protein folding and titration." [S.l. : s.n.], 2000. http://www.diss.fu-berlin.de/2000/124/index.html.

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27

Köberling, Oliver. "Mechanismen der Qualitätskontrolle bei der Sec-abhängigen Proteintranslokation in Gram-positiven Bakterien." [S.l. : s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=970885040.

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28

Baumann, Markus. "Identifizierung aktiver und enantioselektiver Hydrolasen für den industriellen Einsatz." [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=966445929.

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29

Pei, Lei. "A fibrinogen-binding protein from Staphylococcus epidermidis /." Stockholm : [Karolinska institutets bibl.], 2001. http://diss.kib.ki.se/2001/91-7349-059-8/.

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30

Uku, Jacqueline Nduku. "Seagrasses and their epiphytes : characterization of abundance and productivity in tropical seagrass beds /." Stockholm : Dept. of Botany, Stockholm university, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-527.

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31

Niepel, Tanja. "Chemotaxonomie ausgewählter Gram-positiver Bakterien anhand ihrer polaren Lipide, Sequenzierung ihrer 16S rDNAs und Vergleich beider Methoden." [S.l. : s.n.], 1999. http://www.gbv.de/dms/bs/toc/300316259.pdf.

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32

Langhoff, Florian. "Antiseptischer Knochenzement: Freisetzung von Octenidin aus Polymethylmethacrylat (PMMA)." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-65244.

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33

Vestrum, Ragnhild Inderberg. "Funksjonalitet i bakterie/torskelarve interaksjoner." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for bioteknologi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-19556.

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Ved produksjon av torskelarver (Gadus morhua) har man ofte opplevd problemer med lav reproduksjonen blant annet på grunn av bakterielle infeksjoner. For å forsøke å redusere bruken av antibiotika i oppdrettsnæringen forsøker man å finne ulike måter å kontrollere det mikrobielle miljøet rundt fiskelarvene. En type mikrobiell kontroll er tilsetning av probiotiske bakterier til vannet eller fôret for å kolonisere torskelarvene med ønskede bakterier. En annen type mikrobiell kontroll er å hindre patogene bakterier i å skade fiskelarvene ved å forstyrre quorum sensing prosessen. Dette ble foretatt et 17 dagers startfôringsforsøk og et 5 dagers korttidsforsøk. Startfôringsforsøket ble gjennomført i 2 liters flasker med filtrert autoklavert sjøvann (FASV) (30 torskelarver L-1) med aktiv lufting og torskelarvene ble fôret hver dag fra dag 3 etter klekking. Korttidsforsøket ble gjennomført i flasker med 50 mL FASV (30 torskelarver) uten lufting og fôring. Systemene ble holdt gnotobiotiske under hele forsøket. Microbacterium sp., Pseudoalteromonas sp. og Vibrio sp., tre probiotiske kandidater ble undersøkt med hensyn til deres innvirkning på torskelarvers vekst, overlevelse og genuttrykk. Levende Microbacterium sp. og Pseudoalteromonas sp. hadde en negativ effekt på torskelarvenes overlevelse, mens Vibrio sp. hadde ingen effekt i forhold til den bakteriefri kontrollbehandlingen. De probiotiske kandidatene tilsatt som monokulturer hadde ingen probiotisk effekt på torskelarvene. Døde bakterier gav ingen respons med hensyn til torskelarvenes overlevelse, og det ble konkludert med at bakteriene må være levende/aktive for å ha en effekt. 8 ulike gener ble kvantifisert for torskelarvene fra startfôringsforsøket og to av genene ble kvantifisert for larvene fra korttidsforsøket med levende og døde probiotiske bakterier. Fiaf og Fdps er gener som er involvert i fiskens næringsmetabolisme. Uttrykket av disse genene var oppregulert i behandlingene med levende Microbacterium sp. Dette var også tilfellet for Cyp1a1 som er involvert i den xenobiotiske metabolismen. C3 og Gpx2 er begge gener som er koblet til immunsystemet, og disse hadde begge høyest uttrykk i larvene fra den bakteriefrie kontrollbehandlingen, og lavest uttrykk i larvene fra behandlingen med død Microbacterium sp. CLECT er også et gen som er involvert i fiskens immunrespons, men her var det ingen signifikante forskjeller mellom uttrykket i de ulike behandlingene. Det var det heller ikke for WARS, et gen involvert i fiskens proteinsyntese. Itgb1bp3 koder for integrinβ-1-binding protein, og dette var uttrykt høyest i behandlingene med levende Microbacterium sp. For torskelarvene fra korttidsforsøket ble genene C3 og Cyp1a1 kvantifisert, og begge gener var høyest uttrykt hos larvene fra behandlinger med L. anguillarum og Pseudoalteromonas sp. Behandlingene med døde bakterier gav generelt sett lavere respons enn behandlingene med levende bakterier. Potensielle quorum sensing nedbrytende kandidater fra torskefeces ble anriket med cellefritt vann fra L. anguillarum og AHL-molekyler. Tre av anrikingssamfunnene ble benyttet i et korttidsforsøk med L. anguillarum som kjent patogen. Behandling med levende L.anguillarum førte til rask død etter bakterietilsetning, mens tilsetning av død L. anguillarum ikke hadde noen effekt på overlevelsen i forhold til en bakteriefri kontrollbehandling. Tilsats av de anrikede bakteriesamfunnene uten challenge av L. anguillarum hadde ingen effekt på torskelarvenes overlevelse, men alle tre samfunn hadde evnen til å utsette L. anguillarum sin patogene effekt med omtrent 3 dager. Bakteriesamfunnenes positive effekt på torskelarvene kom av deres evne til nedbryting av L. anguillarum’s quorum sensing signalmolekyler.

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34

Pelantová, Lucie. "Klasifikace bakterií pomocí markerových genů." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2020. http://www.nusl.cz/ntk/nusl-433568.

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The aim of this work is proposal of new method for bacteria classification based on sequences of marker genes. For this purpose was chosen 10 marker genes. Resulting MultiGene classifier processes data set by dividing it in several groups and choosing gene for each group which can distinguish this group with best results. This work describes implementation of MultiGene classifier and its results in comparison with other bacteria classifiers and with classification based entirely on gene 16S rRNA.

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35

Melušová, Soňa. "Studium produkce polyhydroxybutyrátu u bakterií." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216477.

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Presented work is focused on study of polyhydroxybutyrate production in bacteria. In theoretical part short characterization of PHB was given and the most common representative of wide group of polyhydroxyalkanoates (PHA) were described. Then, production of PHB and copolymer P(HB-co-HV) in selected bacterial strains was experimentally proven. First, PHB production in Bacillus megaterium using synthetic medium was studied. The PHB content in cells was increased during cultivation under limiting conditions, despite low growth. Addition of ethanol into production media resulted in increased PHB synthesis as well as biomass production (21 % PHB of 1,8 g/l biomass). Further, BM medium containing 8 g/l glucose was tested. PHB production was more than 1 g/l at significant growth increase when compared with synthetic medium. The bacteria B.megaterium showed, except glucose, ability to utilize maltose and xylose. Another cultivations were tested with bacterial strain Azotobacter vinelandii, which is capable of copolymer P(HB-co-HV) synthesis. Maximal growth and copolymer content was reached on Burk's medium with 30 g/l of glucose. Addition of peroxide to growth medium influenced P(HB-co-HV) synthesis to 46 % of 2,6 g/l biomass. Bacteria A.vinelandii showed the best growth on maltose, even compared with glucose (54 % copolymer of biomass content). Finally, PHB production on industrial waste product – whey was monitored. Using Plackett-Burman design for statistical media optimization, the whey content was modified. B.megaterium grown on adjusted whey reached 0,5 g/l PHB, 32 % of cell's content.

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36

Zovčáková, Monika. "Identifikace probiotických bakterií ve farmakách." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2010. http://www.nusl.cz/ntk/nusl-216586.

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Lactobacilli are dominant bacteria of the vaginal flora. Lactobacillus-containing probiotics products are used for the treatement and profylaxis of bacterial urogenital infections. This work is focused on DNA identification and species identification of probiotic bacteria in 5 different vaginal tablets using molecular-genetic methods. Total DNA isolated from complex matrix of vaginal tablets was used for amplification in polymerase chain reaction. DNA was isolated from crude cell lysates by magnetic particles P(HEMA-co-GMA) and by method of phenol extraction. Identification of species of probiotic bacteria was verified using genus-specific and species-specific PCRs. Results of bacterial identification obtained by PCR were compared with declared specification given by producers. Bacteria of genus Lactobacillus were proved in all tablets whereus species identification was in accordance with the stated composition in 1 tablet only.

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37

Skřivanová, Veronika. "Metody identifikace PHA produkujících bakterií." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2016. http://www.nusl.cz/ntk/nusl-240529.

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This diploma thesis deals with testing, optimazing and comparing methods for the identification of bacteria producing polyhydroxyalkanoates. Work included cultivation and microscopy methods, wherein the bacterial cells were stained with lipophilic dyes Nile red and Sudan black. Further, we also used flow cytometry and spectroscopic methods - Raman spectroscopy and infrared spectroscopy with Fourier transformation, and molecular biological methods, which analyzed the presence of a gene encoding PHA synthase (phaC) by polymerase chain reaction (PCR). PCR assay consist of two reactions, the firt on eis based on amplification of phaC gene along with 16S rRNDA gene, which is common for all the bacteria (multiplex PCR). The second reaction is focused on specific amplification of PHA synthase catalyzing biosynthesis of mcl-PHA. In order to overcome false positive results typical for methods analyzing genotype and also to avoid false negative results occuring in fenotype analyzing methods, the best strategy is to combine both aproaches. According to our results, analysis of presence of phaC gene by PCR can be combined with methods capable of determining presence of PHA in bacterial cells. For this purpose, Raman microspectroscopy seems to be very promising tool, since it is able to detect low content of PHA in cells and PHA can not be confused with other lipid metabolites. The results provide an overview of test methods, their advantages and disadvantages and also to compare different criteria according to which it is possible to choose the method of identification in depending on the adjustable requirements.

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38

Černá, Klára. "Charakterizace adsorbčních vlastností probiotických bakterií." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2018. http://www.nusl.cz/ntk/nusl-376799.

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The aim of the diploma thesis is to characterize the adsorption properties of probiotic bacteria. Characterization was performed using of designed and optimized spectrophotometric method of determination of adhesion of probiotic bacteria to mucin as the main viscoelastic component of mucus. In order to provide a more detailed description of the adhesion interactions involved in the adhesion of probiotic bacteria to mucin, the viability of selected probiotic bacterial strains Lactobacillus rhamnosus CCM 1825, Lactobacillus plantarum CCM 7039, Lactobacillus delbrueckii subsp. bulgaricus CCM 7190, Lactobacillus acidophilus CCM 4833, Lactobacillus casei CCM 4798, Bifidobacterium breve CCM 7825, Bacillus coagulans CCM 2658 and a potential probiotic strain of Lactobacillus zeae CCM 7069. Moreover, bacterial adhesion to carbohydrates was also determined for all the bacteria tested. The last proposed and optimized technique was dynamic and electrophoretic light scattering characterizing surface properties such as -potential, average size distribution and isoelectric point. From this method, information was obtained on aggregation of bacterial cells and on electrostatic interactions. The combination of these methods was used as a complex tool to characterize adhesion of the tested bacterial cultures as a very specific, sensitive and key parameter of a successful probiotic microorganism that is influence by the multiple effects.

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Marková, Kateřina. "Vnitřní fluorescence bakterií Cupriavidus necator." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2018. http://www.nusl.cz/ntk/nusl-376824.

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This thesis focuses on autofluorescence of flavins in gram-negative bacteria Cupriavidus necator H16 and its mutant strain PHB-4. The main methods used were fluorescence microscopy and flow cytometry. To confirm the presence of flavins, excitation and emission spectra of the bacterial suspension were measured, which were compared with flavin standards. In the part of testing cells without stress response, the autofluorescence of bacteria in PBS buffer and cell suspensions stained with fluorescence probe BODIPY 493/503 was measured. The ratio of short fluorescence lifetime to long autofluorescence lifetime, and its dependence on fluorescence probe was compared with previous conditions. Autofluorescence of the supernatant was measured; it was found that the relative amplitude of long lifetime was multiple times higher than in the cell. In the part devoted to the stress response, this thesis was focused on the amount of dissolved oxygen in the production medium and the effect on bacterial autofluorescence. Then differently concentrated hydrogen peroxide was used, the best results were obtained from the concentration of 100 mM in media. For comparison a combination of hydrogen peroxide with ferro-ammonium sulphate was used, but there was no big difference. Sodium azide and antimycin A were selected as substances that directly influence on bacterial respiratory chain. Both compounds affected change in the ratio of the relative amplitudes, but the distribution of these lifetimes and the autofluorescence change over time was affected only by sodium azide.

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Nováčková, Ivana. "Evoluční inženýrství bakterií produkujících polyhydroxyalkanoáty." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2018. http://www.nusl.cz/ntk/nusl-376857.

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This diploma thesis deals with the application of evolutionary engineering to PHA producing bacterial strains. The aim of the thesis is to prepare strains adapted to levulinic acid, a selected stress factor, by methods of evolutionary engineering, and then to characterize these strains. The theoretical part deals with evolutionary engineering and polyhydroxyalkanoates predominantly. The bacterial strain Cupriavidus necator H16 was used for evolutionary experiments. Levulinic acid and levulinic acid in the presence of the MMS mutagen were applied to prepare adapted strains. Selection of mutants was evaluated on the basis of growth potential and PHA content in biomass. Polymers produced by five obtained PHA-producing mutants and control were characterized using GC-FID, SEC-MALS, DSC and FT-IR. It was found that a higher content of 3HV in the copolymer led to a lower crystallinity and hence to a lower melting point, nevertheless, only the copolymer of the M0151 strain did not fit this trend. In addition to the characteristics of the polymers, the strains themselves were evaluated from the biochemical point of view by determining the activities of selected enzymes of the citrate, glyoxalate and 2-methylcitrate cycle, selected enzymes generating NADPH, levulic acid catabolism enzyme and PHA biosynthesis enzymes. On the basis of the obtained data, the possible adaptation strategies were discussed, when the E0575 strain was most differentiated from original culture. Values of specific enzyme activities were subjected to AHC and PCA statistical analysis methods.

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Nykrýnová, Markéta. "Genotypizace kmenů bakterie Klebsiella pneumoniae." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2018. http://www.nusl.cz/ntk/nusl-378023.

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This master thesis deals with typing of Klebsiella pneumoniae isolates. The first part of the thesis introduces molecular typing methods. Then bacterial genomes and Klebsiella pneumoniae are characterized. Following part describes data validation, assembly of genomes and proposed algorithm for finding genes with high variability. In last part obtained results are presented and validated on other genomes of Klebsiella pneumoniae.

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Hölzel, Christina. "Antibiotikaresistente Bakterien und Resistenzgene in Schweinegülle." Diss., lmu, 2006. http://nbn-resolving.de/urn:nbn:de:bvb:19-61000.

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Roeßler, Markus. "Chlorid, ein neues Signalmolekül für Bakterien." Diss., lmu, 2002. http://nbn-resolving.de/urn:nbn:de:bvb:19-640.

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Bischoff, Erik. "Schnellnachweis von bierschädlichen Bakterien mit PCR." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964420333.

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Sedláková, Lucie. "Analýza DNA izolované z různých typů probiotických výrobků s využitím PCR v reálném čase a HRM analýzy." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2016. http://www.nusl.cz/ntk/nusl-240562.

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The aim of this diploma thesis was to introduce real-time PCR with high-resolution melting analysis for Bifidobacterium species. Currently a small number of publication, dealing with identification of Bifidobacterium species using high-resolution melting analysis, is available. According to publications dealing with identification of lactic acid bacteria were selected primers P1V1 and P2V1, LAC1 and LAC2, LsppUPF and LsppUPR, V3F and V3R, V6F and V6R. Using this primers bacterial DNA was amplified by real-time PCR with high-resolution melting analysis. After evaluation of the measured results efficiency of selected primers was verified on DNA izolated from complex sample of probiotic product. After further optimisation real-time PCR with high-resolution melting analysis could be suitable using selected primers for Bifidobacterium species.

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Brinck, Kajsa. "Ultraljuds effekt på bakterier : Studie av ultraljuds effekt på bakterier vid olika temperaturer och frekvenser av ultraljud." Thesis, Umeå universitet, Biomedicinsk laboratorievetenskap, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-58622.

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Linnemann, Thomas Vitus [Verfasser]. "Die Phyllosphäre als Lebensraum für Bakterien: Mechanismen der Interaktion epiphyller Bakterien mit der pflanzlichen Kutikula / Thomas Vitus Linnemann." Bonn : Universitäts- und Landesbibliothek Bonn, 2020. http://d-nb.info/122496618X/34.

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Siegl, Alexander. "Einzelzell-basierte Methoden zur Charakterisierung Schwamm-assoziierter Bakterien." kostenfrei, 2009. http://www.opus-bayern.de/uni-wuerzburg/volltexte/2009/3744/.

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Wilhelm, Cornelia. "In-Vitro Wirksamkeit von Moxifloxacin und Linezolid gegen Staphylococcus aureus-, Streptococcus pneumoniae- und Enterococcus spp.-Isolate in Abhängigkeit vom Testmedium und der Keimlokalisation /." Wettenberg : VVB Laufersweiler, 2004. http://deposit.d-nb.de/cgi-bin/dokserv?idn=974094218.

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Šťásková, Lucie. "Vybrané bioinženýrské charakteristiky bakterií mléčného kvašení." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2012. http://www.nusl.cz/ntk/nusl-216825.

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The diploma thesis deals with the growth of biomass and production of selected metabolit–lactic acid by thermophilic bacteria Bacillus coagulans. The resulting selected metabolite was determined by HPLC method. Cultivations of this genus were performed on synthetic media, where the influence of carbohydrate used as carbon source was tested. Lactose was more suitable fot growth of biomass and glucose for production of lactic acid. On natural whey media the influence of different conditions were tested. The highest yields of biomass and production of lactic acid were observed on enriched whey medium. The last part deals with comparing the production of biomass and metabolites, depending on the volume of media. There were compared selected bioengineering characteristics of all cultivations.

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