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1

Arora, Nishtha. "Comparative evaluation of nano-filled and conventional adhesives for bonding of molar tubes." Bioinformation 17, no. 4 (April 30, 2021): 492–99. http://dx.doi.org/10.6026/97320630017492.

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It is of interest to compare the bonding characteristics of the two nano filled adhesives, Grandio (Voco, Cuxhaven, Germany) and Transbond Supreme LV (TSLV, 3M Unitek, Monrovia, California) with conventional bonding adhesive Transbond XT (TBXT, 3M Unitek) for bonding of molar tubes. 45 extracted human permanent molar teeth, divided into three groups of 15 each, were bonded with stainless steel molar tubes (3M Unitek, USA) using TBXT in Group 1, Grandio in Group 2, TSLV in Group 3. Remnant Index and shear bond strength was evaluated after 24 hrs. of storage with the aid of Instron Universal testing machine and Stereomicroscope respectively. Data were analysed using Analysis of Variance (ANOVA) test, Post-hoc Bonferroni test and Kruskal Wallis test. The mean SBS of Group 1(TBXT) was 13.86±3.27 MPa, Group 2 (Grandio) was 9.48±2.36 MPa and Group 3 (TSLV) was 11.64±2.71 MPa. Both nano-filled adhesives had SBS well above the clinically acceptable range. Assessment of ARI scores and type of bond failure revealed that adhesive failure for TBXT and TSLV and cohesive failure for Grandio. Nano-filled adhesives can be an appropriate substitute for the conventional adhesive for bonding of molar tubes.
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2

Colombo, Marco, Claudio Poggio, Alessandro Lasagna, Marco Chiesa, and Andrea Scribante. "Vickers Micro-Hardness of New Restorative CAD/CAM Dental Materials: Evaluation and Comparison after Exposure to Acidic Drink." Materials 12, no. 8 (April 16, 2019): 1246. http://dx.doi.org/10.3390/ma12081246.

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CAD/CAM (computer-aided design/computer-aided manufacturing) for indirect restorative materials has been recently introduced in dentistry. The purpose of this study was to evaluate the change of the surface micro-hardness of different restorative CAD/CAM materials after exposure to a carbonated acidic drink (Coca-Cola, Coca-Cola Company, Milan, Italy). One hundred and eighty specimens of identical size (2 mm thickness) were obtained by sectioning each tested CAD/CAM block of four materials: a hybrid ceramic (CERASMART™, GC Corporation, Tokyo, Japan), a resin nano ceramic (Lava™ Ultimate, 3M, Monrovia, CA, USA), a nanohybrid composite (Grandio blocs, VOCO GmbH, Cuxhaven, Germany), and a zirconia-reinforced lithium silicate glass ceramic (VITA SUPRINITY® PC; VITA Zahnfabrik, Bad Sackingen, Germany). Forty-five specimens of each material were tested. Micro-hardness was measured at baseline, after 7 days and after 28 days. The data were analyzed. The micro-hardness of each material varied significantly after immersion in Coca-Cola. The nanohybrid composite had a high initial micro-hardness and the greatest percentage loss after acid exposure. The hybrid ceramic and the resin nano ceramic had similar percentage losses of micro-hardness values even if the second material had higher initial values. The zirconia-reinforced lithium silicate glass ceramic had the highest baseline values and the lowest percentage loss of micro-hardness. The different CAD/CAM materials presented different micro-hardness values before and after acid exposure.
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Elsharkasi, MM, JA Platt, NB Cook, GH Yassen, and BA Matis. "Cuspal Deflection in Premolar Teeth Restored with Bulk-Fill Resin-Based Composite Materials." Operative Dentistry 43, no. 1 (January 1, 2018): E1—E9. http://dx.doi.org/10.2341/16-072-l.

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SUMMARY The present study investigated the effect of three high-viscosity bulk-fill resin-based composite materials on cuspal deflection in natural teeth. Thirty-two sound maxillary premolar teeth with large slot mesio-occlusal-distal cavities were distributed into four groups (n=8). Three groups were restored with bulk-fill resin composite materials (Tetric EvoCeram Bulk Fill, Ivoclar Vivadent, Schaan, Liechtenstein; x-tra fil, VOCO, Cuxhaven, Germany; and SonicFill, Kerr, Orange, CA, USA) in a single 4-mm increment. The conventional composite group, Filtek Z100 (3M ESPE, St Paul, MN, USA), was used to restore the cavities in 2-mm increments. Cusp deflection was recorded postirradiation using a Nikon measurescope UM-2 (Nikon, Tokyo, Japan) by measuring the changes in the bucco-palatal widths of the teeth at five minutes, 24 hours, and 48 hours after completion of the restorations. Cuspal deflection was significantly higher in the conventional composite than in the Tetric EvoCeram Bulk Fill (p=0.0031), x-tra fil (p=0.0029), and SonicFill Bulk (p=0.0002) groups. There were no significant differences in cuspal deflection among the three bulk-fill materials (all p<0.05). In conclusion, all the investigated bulk-fill resin composites exhibited cuspal deflection values that were smaller than those associated with a conventional incrementally placed resin composite.
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Steinmetz, D., J. Winsemann, C. Brandes, B. Siemon, A. Ullmann, H. Wiederhold, and U. Meyer. "Towards an improved geological interpretation of airborne electromagnetic data: a case study from the Cuxhaven tunnel valley and its Neogene host sediments (northwest Germany)." Netherlands Journal of Geosciences - Geologie en Mijnbouw 94, no. 2 (December 30, 2014): 201–27. http://dx.doi.org/10.1017/njg.2014.39.

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AbstractAirborne electromagnetics (AEM) is an effective technique for geophysical investigations of the shallow subsurface and has successfully been applied in various geological settings to analyse the depositional architecture of sedimentary systems for groundwater and environmental purposes. However, interpretation of AEM data is often restricted to 1D inversion results imaged on resistivity maps and vertical resistivity sections. The integration of geophysical data based on AEM surveys with geological data is often missing and this deficiency can lead to uncertainties in the interpretation process. The aim of this study is to provide an improved methodology for the interpretation of AEM data and the construction of more realistic 3D geological subsurface models. This is achieved by the development of an integrated workflow and 3D modelling approaches based on combining different geophysical and geological data sets (frequency-domain helicopter-borne electromagnetic data (HFEM), time-domain helicopter-borne electromagnetic data (HTEM), three 2D reflection seismic sections and 488 borehole logs). We used 1D inversion results gained from both HFEM and HTEM surveys and applied a 3D resistivity gridding procedure based on geostatistical analyses and interpolation techniques to create continuous 3D resistivity grids. Subsequently, geological interpretations have been performed by combining with, and validation against, borehole and reflection seismic data. To verify the modelling results and to identify uncertainties of AEM inversions and interpretation, we compared the apparent resistivity values of the constructed 3D geological subsurface models with those of AEM field measurements. Our methodology is applied to a test site near Cuxhaven, northwest Germany, where Neogene sediments are incised by a Pleistocene tunnel valley. The Neogene succession is subdivided by four unconformities and consists of fine-grained shelf to marginal marine deposits. At the end of the Miocene an incised valley was formed and filled with Pliocene delta deposits, probably indicating a palaeo-course of the River Weser or Elbe. The Middle Pleistocene (Elsterian) tunnel valley is up to 350 m deep, 0.8–2 km wide, and incised into the Neogene succession. The unconsolidated fill of the Late Miocene to Pliocene incised valley probably formed a preferred pathway for the Pleistocene meltwater flows, favouring the incision. Based on the 3D AEM resistivity model the tunnel-valley fills could be imaged in high detail. They consist of a complex sedimentary succession with alternating fine- and coarse-grained Elsterian meltwater deposits, overlain by glaciolacustrine (Lauenburg Clay Complex) and marine Holsteinian interglacial deposits. The applied approaches and results show a reliable methodology, especially for future investigations of similar geological settings. The 3D resistivity models clearly allow a distinction to be made between different lithologies and enables the detection of major bounding surfaces and architectural elements.
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5

Ünal, Mehmet, and Fatma Atakul. "The evaluation of effectiveness of adhesive systems on dental amalgam restorations." International Dental Research 11, no. 2 (August 31, 2021): 83–92. http://dx.doi.org/10.5577/intdentres.2021.vol11.no2.5.

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Aim: The purpose of this study was to evaluate the effectiveness of different adhesive systems in amalgam restorations and their effects on microleakage. Methodology: In this study, 105 caries-free extracted human permanent molar teeth were used. Teeth were randomly assigned to five groups (n=21), and class I cavities were created on the surface of each tooth. The first was a control group to which no adhesive system was applied. Amalgam Liner (VOCO GmbH, Cuxhaven Germany) was applied to Group II, Clearfil SE-Bond (Kuraray Europe GmbH, Frankfurt Germany) was applied to Group III, Panavia F 2.0 (Kuraray Europe GmbH, Frankfurt Germany) was applied to Group IV, Amalgambond Plus (Parkell Inc.Edgewood, NY USA) was applied to Group V, and then amalgam (Tytin, Kerr, California USA) restorations were placed. After the polishing process, samples were subjected to thermocycling 1,000 times. Teeth were sectioned bucco-palatinally/lingually, and microleakage scores of the occlusal walls were evaluated under a stereomicroscope at 15X magnification by a standardized scale ranging from 0 to 4. One tooth was selected randomly from each group for SEM (Scanning Electron Microscope), and SEM LEO EVO 40 (LEO Ltd., Cambridge UK) photographs of amalgam-tooth hard tissue interfaces were also taken at different magnifications. The results of the microleakage tests were statistically analyzed by both the Kruskal-Wallis Test and the Mann Whitney U Test. Results: In terms of microleakage among groups, the differences that were determined were significant (p<0.05). Microleakage within the control group was determined to be the highest, and statistically important differences were observed between the other groups. Group V (Amalgambond Plus) was determined to have the lowest microleakage scores. Conclusion: In prepared class I cavities, amalgam adhesive systems are effective in preventing occlusal microleakage but do not completely blocked it. How to cite this article: Ünal M, Atakul F. The evaluation of effectiveness of adhesive systems on dental amalgam restorations. Int Dent Res 2021;11(2):83-92. https://doi.org/10.5577/intdentres.2021.vol11.no2.5 Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.
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6

Kacprzak-Ogłuszka, Marzena, and Beata Dejak. "Bond strength of glass fibre-reinforced posts with composite cement." Prosthodontics 68, no. 2 (May 15, 2018): 232–42. http://dx.doi.org/10.5604/01.3001.0012.0492.

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Introduction. The most common failure in using glass fibre-reinforced composite posts is cementation of the post, i.e. the lack of junction between cement dentine or post. Aim of the study. To compare the bond strength between different glass fibre-reinforced composite (FRC) posts and selected composite cement. Materials and Methods. The following seven types of fiber reinforced composite posts (n =10): Fiber Post (GC Europe N.V., Leuven, Belgium), Glassix (Harald Nordin SA, Chailly s/ Montreux, Switzerland), Rebilda®Post (VOCO GmbH, Cuxhaven, Germany), FibreKleer Serrated Post (Pentron Clinical, Wallingford, USA), ParaPost®Fiber Lux (Coltene/Whaledent Inc., Cuyahoga Falls, USA), RelyX (3M Espe Dental Products, St.Paul, USA) oraz X-Post (Dentsply Maillefer Instruments, Ballaigues, Switzerland) were evaluated. Posts were cemented into metal sleeves with dual-cure resin cement CoreXFlow (Dentsply). The bond strength test based on the coaxial method was performed on the samples using Instron 4485 machine (Instron Corporation, Norwood, USA). Results. The significantly higher values of bond strength were demonstrated for Fiber Post (GC Europe N.V., Leuven, Belgium) with 29.57±1.44 MPa, and the lowest were shown by Glassix (Harald Nordin SA, Chailly s/Montreux, Switzerland) with 17.21±0.87 MPa. The differences were significant (p<0.001). Conclusions. 1. The significantly higher values of bond strength were demonstrated for Fiber Post (GC Europe N.V., Leuven, Belgium) and the lowest were shown by Glassix (Harald Nordin SA, Chailly s/Montreux, Switzerland). 2. The resin methacrylic posts had a stronger junction with cement than with epoxy resin posts.
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Beltrami, Riccardo, Marco Colombo, Andrea Cavada, Sofia Panizzi, Claudio Poggio, and Andrea Scribante. "Influence of Enamel Exposure to Acidic Drink on Shear Bond Strength of Different Fissure Sealants." Bioengineering 9, no. 1 (January 8, 2022): 20. http://dx.doi.org/10.3390/bioengineering9010020.

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In the present study, we evaluated the influence of bovine enamel exposure to acidic drinks (Coca-Cola, Coca-Cola Company, Milano, Italy, pH = 2.37) on shear bond strength of three sealants (Fissurit; Grandio Seal and Admira Fusion—Voco Gmbh, Cuxhaven, Germany). For each sealant, two adhesive techniques were tested to investigate the impact of the adhesive application on shear bond strength of sealants after immersion in acidic drink and in the control: Group 1—Control: enamel surface was not in contact with acid drinks, acid etching application and self-adhesive technique for fissure sealant; Group 2—enamel surface was not in contact with acid drinks, acid etching, and adhesive applications, an etch-and-rinse technique for fissure sealant; Group 3—enamel surface was immersed in acid drink, acid etching application and self-adhesive technique for fissure sealant; Group 4—enamel surface was immersed in acid drink, acid etching, and adhesive applications, an etch-and-rinse technique for fissure sealant. For each specimen, the sealant composite resin was applied to the enamel surface and tested with a universal testing machine. Shear bond strength was measured in MPa and with an optical microscope to determine failure modes, quantified with adhesive remnant index (ARI). Enamel acidification variably influenced bond strength values of the different sealants. When no enamel pretreatment was applied, no significant differences were found among the sealants (p > 0.05). However, the mere application of acid etching without adhesive procedures resulted in lower bond strength (p < 0.001). The acid pretreatment affected significantly the bond strength of all sealants tested (p < 0.001), but no significant differences were recorded between the subgroups.
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Demirel, G., AI Orhan, O. Irmak, F. Aydın, A. Büyüksungur, B. Bilecenoğlu, and K. Orhan. "Effects of Preheating and Sonic Delivery Techniques on the Internal Adaptation of Bulk-fill Resin Composites." Operative Dentistry 46, no. 2 (March 1, 2021): 226–33. http://dx.doi.org/10.2341/19-241-l.

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SUMMARY Objective: To compare the effects of conventional (hand-placed), sonic, or preheated insertion techniques on the internal adaptation of bulk-fill resin composites. Methods and Materials: A total of 150 freshly extracted human third molars were used to prepare standardized cylindrical occlusal cavities. Teeth were divided into five main groups according to the resin composites: 1 incremental (Clearfil Majesty Posterior [CMP]) and four paste-like bulk-fill (SonicFill 2 [SF2], VisCalor bulk [VCB], Filtek One bulk-fill restorative [FBR], and Tetric EvoCeram bulk-fill [TEB]). Each main group was divided into three subgroups according to the placement technique: conventional, preheating, and sonic delivery (n=10). In the conventional placement technique, cavities were filled manually. In the sonic insertion technique, a specific handpiece (SonicFill Handpiece; Kerr Corporation) was used. In the preheating technique, a heating device (Caps Warmer, Voco, Cuxhaven, Germany) was used to warm the resin composites before placement. Internal voids (%) of the completed restorations were calculated with microcomputed tomography. Data was analyzed with two-way analysis of variacne followed by Tukey’s multiple comparisons test (α=0.05). Results: All resin composites showed fewer internal gaps with preheating compared with the conventional placement (p&lt;0.05). For all resin composites other than SF2, preheating provided fewer internal gaps than that of the sonic placement (p&lt;0.05). Sonic placement led to fewer internal gaps compared with the conventional placement, but only for SF2 and FBR (p&lt;0.05). For the conventional placement, the lowest gap percentage was observed with the incremental resin composite (CMP, p&lt;0.05). Among all groups, the lowest gap percentages were observed for preheated VCB followed by sonically inserted SF2 (p&lt;0.05). Conclusion: The best internal adaptation was observed in sonically inserted SF2 and preheated VCB, which were the manufacturers’ recommended insertion techniques. Preheating considerably improved the internal adaptation of all resin composites, except for that of SF2.
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Çağırır Dindaroğlu, Funda, and Ece Yılmaz. "Two-year evaluation of a nano-hybrid and a bulk-fill resin composite: a randomized, double-blind split-mouth clinical study." Clinical Oral Investigations 28, no. 4 (March 11, 2024). http://dx.doi.org/10.1007/s00784-024-05592-8.

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Abstract Objectives The aim of this study was to compare the 2-year clinical performance of a bulk-fill composite resin and a nano-hybrid-filled composite resin in 6-12-year-old children in a split-mouth design. Materials and methods This randomized, split-mouth, and double-blind study was conducted on 89 patients aged 6–12 years with caries on bilateral mandibular first molars. In a split-mouth design, restorations of mandibular permanent molars were completed with nano-hybrid organically modified ceramic (ORMOCER)-based bulk-fill composite resin Admira Fusion x-tra (Voco GmbH, Cuxhaven, Germany) and nano-hybrid composite Grandio (Voco, Cuxhaven, Germany). Futurabond U single dose (Voco, Cuxhaven, Germany) was used with selective enamel etching. The clinical success of the restorations was evaluated using USPHS and FDI criteria at 6, 12, and 24-month follow-up controls. Results In the 2-year follow-up, all restorations were clinically acceptable. Grandio was significantly worse than Admira Fusion x-tra in terms of surface luster and superficial change (p < 0.05). Surface staining and color match scores increased in Admira Fusion x-tra compared with Grandio significantly (p < 0.05). Conclusions Although both materials showed acceptable clinical performance over 2 years, a significant difference was observed between the surface luster, surface staining, marginal adaptation, and staining of the nano-hybrid composite placed with the incremental technique and the bulk-fill ORMOCER-based composite resin. Clinical relevance As an alternative to nano-hybrid composite resins, using bulk-fill restorative materials, which can be indicated in the proper case, may contribute to shortening treatment procedures and increasing patient and physician comfort, leading to clinical success.
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ZİNCİR, Bahar, Elif ŞENAY, Ece ÖZTOPRAK, and Levent ÖZER. "In-vitro bond strength of a flowable compomer to primary teeth dentin with different applying techniques." European Annals of Dental Sciences, August 30, 2022. http://dx.doi.org/10.52037/eads.2022.0031.

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Purpose: The study aimed to investigate the effect of applying flowable compomer using different techniques and long-term water storage on microtensile bond strength (µTBS) to primary teeth dentin and present a comparison with packable compomer. Materials and methods: 90 primary molars were used to evaluate the µTBS of the materials. Specimens were randomly divided into 3 main groups for restoration: Group 1 and 4, compomer (Glasiosite, VOCO GmbH, Cuxhaven, Germany) filling alone; Group 2 and 5, flowable compomer (Twinky Star Flow, VOCO GmbH, Cuxhaven, Germany) lining/pre-cured and overlaying compomer; and Group 3 and 6, flowable compomer lining/co-cured with overlaying compomer. All specimens were thermocycled (500X), after which half of them (n=45) were stored in distilled water for 24 h (Group A: Groups 1-3), and the remaining half (n=45) for 24 months (Group B: Groups 4-6). Samples were tested for µTBS (1 mm/min) with a microtensile testing machine (T 61010 Ki, Bisco, Schaumburg, USA). Failure modes were determined with the aid of a stereomicroscope. Results were analyzed using the Kruskal-Wallis H test. Results: There was no statistically significant difference between the groups regarding bond strength (p>0.05). Mixed cohesive failure of both adhesive and dentin was the most common type of failure in all groups (p
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Kosan, Esra, Ana Prates-Soares, Uwe Blunck, Konrad Neumann, and Kerstin Bitter. "Root canal pre-treatment and adhesive system affect bond strength durability of fiber posts ex vivo." Clinical Oral Investigations, June 14, 2021. http://dx.doi.org/10.1007/s00784-021-03945-1.

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Abstract Objectives To investigate the effect of different pre-treatments on the long-term bond strength of fiberglass posts luted either with dual-curing self-etch adhesives and core build-up composites or with a self-adhesive resin (SAR) cement. Materials and methods In total, 180 human root-filled teeth received post-space preparations and three different dentin pre-treatments (PTs): PT1, ethanol (99%); PT2, ethanol-tertiary-butanol-water-solution (AH Plus Cleaner, Dentsply Sirona; York, USA); and PT3, distilled water (control). Five luting systems were used: FU, Futurabond U (Voco; Cuxhaven, Germany); CL, Clearfil DC Bond (Kuraray Noritake; Okayama, Japan); GR, Gradia Core SE Bond (GC Europe NV; Leuven, Belgium); LU, LuxaBond Universal (DMG; Hamburg, Germany); and RX, RelyX Unicem 2 (3M; Minnesota, USA). Roots were cut into six slices (1 mm thick). From each root canal region, three slices were submitted to immediate and three to post-storage push-out testing. The latter were subjected to thermocycling (5–55°C, 6.000 cycles) and stored for six months in saline solution (0.9%, 37°C). Data were analysed using repeated measures ANOVA and chi-square tests (MV±SD). Results Bond strength was significantly affected by material (p<0.0005), pre-treatment (p=0.016), and storage (p<0.0005; repeated-measures ANOVA). LU (18.8±8.1MPa) revealed significantly higher bond strength than RX (16.08±6.4MPa), GR (15.1±4.6MPa), CL (13.95±5.2MPa), and FU (13.7±6.3MPa). PT1 (16.5±6.9MPa) revealed significantly higher bond strength than PT3 (14.5±5.7MPa). Conclusions A universal adhesive in self-etch mode combined with a core build-up material revealed higher bond strength than a SAR cement, both interacted positively with Ethanol pre-treatment. Clinical relevance statement Ethanol (99%) rinsing can be recommended as part of post and core pre-treatment for the investigated luting systems.
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Duruk, Gülsüm, Sibel Akküç, and Yılmaz Uğur. "Evaluation of residual monomer release after polymerization of different restorative materials used in pediatric dentistry." BMC Oral Health 22, no. 1 (June 13, 2022). http://dx.doi.org/10.1186/s12903-022-02260-9.

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Abstract Background The choice of the restorative resin material to be used in pediatric dentistry is of a great importance due to the cytotoxic effects caused by residual monomers. In this study, it was aimed to investigate the amount of residual monomer released over time from different resin-based restorative materials, which are widely used in pediatric dentistry, by using high performance liquid chromatography with photodiode array detector (HPLC–PDA). Methods The compomers in all colors (Twinky Star and Glasiositte A2), two composites with different hybrid properties (Arabesk-GrandioSO), and RMGIC (Ionolux) samples with 2 × 5 mm diameters were prepared. The samples were polymerized with an LED light unit (CELALUX 2, VOCO, Cuxhaven, Germany) and then finishing-polishing procedures were applied. A total of 156 samples were obtained, 13 samples in each of the 12 groups. The amount of residual monomer (BIS-GMA; HEMA, TEGDMA, UDMA) (µg/mL) released into the 75% ethanol solution was determined at different times, (1st hour, 1st, 7th, 14th, and 21st day) by using HPLC–PDA. Results The residual monomer release continued on day 21 and BIS-GMA was the most released monomer in all groups. HEMA release showed a maximum increase in all the materials at day 7. The highest amount of residual monomer was detected in the gold-colored compomer. HEMA and BIS-GMA release from RMGIC was less than others in all time frames. Conclusions The color and composition of resin-based restorative materials affect the amount of residual monomer. Pediatric dentists should prefer gold-colored compomers less than others as a restorative material, especially in deep cavities. More studies are needed about the subject.
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Jhunjhunwala, Garima, Nitesh Tewari, Mohammad Atif, Rahul Morankar, Vijay Prakash Mathur, and Kalpana Bansal. "Comparative evaluation of three materials used for fragment reattachment in uncomplicated crown fracture—An in vitro study using bovine teeth." Dental Traumatology, September 20, 2023. http://dx.doi.org/10.1111/edt.12888.

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AbstractBackground/AimsThe recommended treatment for uncomplicated crown fractures is bonding the fractured fragment or the fragment reattachment. A paucity was identified regarding the studies comparing the efficacy of micro‐hybrid and nanohybrid composites in fragment reattachment. Hence, the present study aimed to evaluate and compare three materials for bonding of fragments rehydrated by humidification in teeth with uncomplicated crown fractures.Material and MethodsEighty mandibular bovine incisors with similar dimensions and free of any structural deformities were fractured similar to the technique followed in previous studies. Fracture was simulated, fragments, and stumps were coded, stumps were stored in artificial saliva and the fragments were dehydrated at room temperature and pressure. They were randomly assigned to Group‐1 (no rehydration), Group‐2 (rehydrated and bonded by flowable nanohybrid composite—3M Filtek Supreme Syringe Flowable Composite Resin—A2, Sao Paulo, Brazil), Group‐3 (rehydrated and bonded by flowable micro‐hybrid composite‐ Ruby Flow, InciDental, England, United Kingdom), and Group‐4 (rehydrated and bonded by light‐cured Glass‐Ionomer‐Cement—Voco Ionoseal, Cuxhaven, Germany). The samples were subjected to a universal testing machine to evaluate the force required to fracture the bonded fragments.ResultsThe highest median value of the force required to fracture was recorded for Group 2 (208.4 N) followed by Group 3 (195.2). The force required to fracture the bonded fragments was lowest in Group 4 (67.2 N) which was lower than the negative control (131.4 N). The differences between the observations in Groups 2 and 3 were not found to be statistically significant.ConclusionThe nano and micro‐hybrid composites showed greater force required to fracture than fragments bonded by LC‐GIC. Dehydrated fragments bonded using nanocomposites performed better than rehydrated fragments bonded by using LC‐GIC.
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Danesh Mesgaran, Mohsen, Hassan Kargar, Sadjad Danesh Mesgaran, and Ali Javadmanesh. "Peripartal Rumen-Protected L-Carnitine Manipulates the Productive and Blood Metabolic Responses in High-Producing Holstein Dairy Cows." Frontiers in Veterinary Science 8 (December 24, 2021). http://dx.doi.org/10.3389/fvets.2021.769837.

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This study aimed to monitor the effect of including rumen-protected L-carnitine (Carneon 20 Rumin-Pro, Kaesler Nutrition GmbH, Cuxhaven, Germany) in the transition diet on the productive and metabolic responses of multiparous high-producing Holstein dairy cows. Thirty-two multiparous cows were allocated in a completely randomized design to receive the same diet plus 60 g fat prill containing 85% palmitic acid (control, n = 16) or 100 g rumen-protected L-carnitine (RLC, n = 16); at 28 days before expected calving until 28 days in milk (DIM). Fat prill was included in the control diet to balance the palmitic acid content of both experimental diets. Milk production over the 28 DIM for the control and RLC groups was 46.5 and 47.7 kg, respectively. Milk fat content tended to increase upon rumen-protected L-carnitine inclusion (p = 0.1). Cows fed rumen-protected L-carnitine had higher fat- and energy-corrected milk compared with the control group. Pre- and post-partum administration of L-carnitine decreased both high- and low-density lipoprotein concentrations in peripheral blood of post-partum cows. The results of this study indicated that the concentration of triglycerides and beta-hydroxybutyrate was not significantly different between the groups, whereas the blood non-esterified fatty acid concentration was markedly decreased in cows supplemented with L-carnitine. Animals in the RLC group had a significant (p &lt; 0.05) lower blood haptoglobin concentration at 7 and 14 DIM than the control. Animals in the RLC group had a lower concentration of blood enzymes than those of the control group. The mRNA abundance of Toll-like receptors 4, cluster of differentiation 14, and myeloid differential protein 2 did not significantly change upon the supplementation of L-carnitine in the transition diet. In summary, the dietary inclusion of RLC improved dairy cow's performance during the early lactation period. Greater production, at least in part, is driven by improved energy utilization efficiency and enhanced metabolic status in animals during the periparturient period.
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Cawthraw, Shaun A., Adam Goddard, Tom Huby, Isaac Ring, Louise Chiverton, and Doris Mueller-Doblies. "Early vaccination of laying hens with the live bivalent Salmonella vaccine AviPro™ Salmonella DUO results in successful vaccine uptake and increased gut colonization." Frontiers in Microbiology 14 (January 15, 2024). http://dx.doi.org/10.3389/fmicb.2023.1327739.

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IntroductionSalmonella Enteritidis and S. Typhimurium are the two most clinically important zoonotic Salmonella serovars and vaccination of breeding and laying hens affords effective Salmonella control. The use of live vaccines has proven beneficial for a number of reasons, including ease of application, protection from the first day of life onwards and initiation of a strong local immune response. Live vaccines can be applied in the drinking water from the first day of life onwards, but some rearers choose to wait until the end of the first week to ensure sufficient water consumption. However, this practice leaves the birds unprotected during the crucial first week of life, where they are most susceptible to colonization by field strains. The aim of this study was to determine if successful vaccine uptake is achieved when layer pullets are vaccinated as early as day one.MethodsThree pullet flocks were vaccinated at 1, 2, 3 or 5 days-of-age with AviPro™ Salmonella DUO, a live vaccine containing attenuated strains of S. Enteritidis and S. Typhimurium (Elanco Animal Health, Cuxhaven, Germany). The vaccine was administered via the drinking water following manufacturer’s instructions. Two days post-vaccination, 10 birds per flock were culled and caecal and liver samples taken, along with two pools of faeces per flock. Levels of vaccine strains were determined by quantitative and qualitative bacteriology.ResultsVaccine strains were detected in all birds from all age groups indicating successful uptake of the vaccine. Levels of the S. Enteritidis vaccine were higher than levels of the S. Typhimurium vaccine, with the latter frequently only detectable following enrichment. There was an inverse correlation between age and caecal levels of vaccines, with the highest numbers seen in birds vaccinated at 1-day-of-age. Interestingly, S. Enteritidis vaccine strain levels in liver samples were highest when birds were vaccinated at 5 days-of-age.DiscussionThese results show that successful uptake of both vaccine strains was evident in all age groups. The earlier the chicks were vaccinated, the higher the vaccine levels in caecal contents. We therefore recommend vaccination of pullets as early as practicably possible to ensure protection against exposure to field strains.
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