Academic literature on the topic 'Bacteriology'

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Journal articles on the topic "Bacteriology"

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Bieluch, Virginia M., Werner D. Chasin, Elizabeth T. Martin, and Francis P. Tally. "Recurrent Tonsillitis: Histologic and Bacteriologic Evaluation." Annals of Otology, Rhinology & Laryngology 98, no. 5 (May 1989): 332–35. http://dx.doi.org/10.1177/000348948909800503.

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Histologic and bacteriologic evaluations of tonsils removed at surgery from ten patients with a diagnosis of recurrent tonsillitis were performed. The bacteriology was complex, with an average of 6.3 aerobic bacteria and 3.3 anaerobic bacteria isolated from each patient. Histologic sections revealed chronic cryptitis, with intact tonsillar architecture. These findings provide a possible explanation for the failure of commonly used antibiotic regimens to eradicate recurrent infection from this site.
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Wani, S. A. "Veterinary bacteriology." Applied Biological Research 16, no. 1 (2014): 124. http://dx.doi.org/10.5958/0974-4517.2014.00060.3.

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Gray, Steven G. "Comparative bacteriology." Trends in Genetics 17, no. 11 (November 2001): 626. http://dx.doi.org/10.1016/s0168-9525(01)02534-3.

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Willcox, M. D. P., and F. Stapleton. "Ocular bacteriology." Reviews in Medical Microbiology 7, no. 3 (July 1996): 123–32. http://dx.doi.org/10.1097/00013542-199607000-00001.

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Richardson, F. W. "Industrial Bacteriology." Journal of the Society of Dyers and Colourists 29, no. 1 (October 22, 2008): 5–9. http://dx.doi.org/10.1111/j.1478-4408.1913.tb00680.x.

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Kerr, J. "Medical Bacteriology." Journal of Clinical Pathology 57, no. 12 (November 24, 2004): 1343.1–1343. http://dx.doi.org/10.1136/jcp.2004.019372.

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Lenart, Kate. "Bacteriology and Sanitation:." Delaware Journal of Public Health 4, no. 2 (March 2018): 87. http://dx.doi.org/10.32481/djph.2018.03.015.

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Fudge, Alan M. "Bacteriology: Another Viewpoint." Journal of the Association of Avian Veterinarians 4, no. 2 (1990): 90. http://dx.doi.org/10.2307/30136885.

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Haeggstrom, A. "BACTERIOLOGY IN PERITONSILLITIS." Pediatric Infectious Disease Journal 6, no. 10 (October 1987): 948–50. http://dx.doi.org/10.1097/00006454-198710000-00031.

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Cossart, Pascale, David Holden, and Stephen Busby. "The new bacteriology." Philosophical Transactions of the Royal Society B: Biological Sciences 371, no. 1707 (November 5, 2016): 20150507. http://dx.doi.org/10.1098/rstb.2015.0507.

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Dissertations / Theses on the topic "Bacteriology"

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Ferreira, Natália Cristina [UNESP]. "Condições bacteriológicas da superfície das poltronas hematológicas par hemodiálise." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/113878.

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Os estabelecimentos de saúde e as superfícies inanimadas que cercam o paciente guardam íntima relação com as infecções relacionadas a assistência e a saúde, sendo importantes focos de contato e de transmissão de agentes patogênicos e podem ser transferidos para as mais diversas superfícies através do contato direto e indireto. Existem inúmeras pesquisas que retratam que os artigos hospitalares podem se tornar veículos de disseminação, comprometendo a segurança do paciente. Diante desta problemática, este estudo objetivou-se em analisar as condições bacteriológicas de superficie de poltronas hematológicas para hemodiálise. Após aprovação do Comitê de Ética em pesquisa foi realizada a randomização das poltronas, duplo cego e iniciou a amostragem das quatro cadeira nos sete procedimentos em cinco locais diferentes na poltrona. O produto utilizado para higienização de rotina na unidade foi o álcool a 70% com compressa hospitalar composta de tecido 100% algodão em dimensão de 30 x 30 cm, umedecida com 40 ml de álcool 70%. Para amostragem bacteriológica utilizou-se campo estéril fenestrado para garantir coleta sempre no mesmo local utilizando Swab. O Swab foi transportado para o laboratório em meio de cultura gel Stuart para identificação. Após foi realizado teste de antibiograma das bactérias isoladas e teste de sensibilidade “in vitro” utilizando método de Kirby e Bauer com modificações conforme descrito por Corrêa (1988). Para o teste “in vitro” utilizou-se três desinfetantes frente as bactérias isoladas das poltronas que são consideradas de grande importância no controle de infecção hospitalar. O resultado demostrou que a maior unidade formadora de colônia foi em relação ao Staphilococcus sp, seguido de bacilus Gram positivo, Staphilococcus aureus, Pseudomonas, Enterobacter cloacae e Acinetobacter, com predominância do Staphilococcus sp em todas as ...
Health facilities and inanimate surfaces surrounding the patient keep close relation with infections related to care and health, with important centers of contact and transmission of pathogens and can be transferred to many different surfaces through direct and indirect contact. There are numerous studies that show that the hospital items can become vehicles of dissemination, compromising patient safety. Faced with this problem, this study aimed to analyze the bacteriological conditions in the surface of hematologic arm for hemodialysis. After approval by the Research Ethics Committee of the randomization arm, double- blind trial was performed and started sampling the four chair the seven procedures in five different locations in the chair. The product used for routine cleaning of the unit was 70% alcohol with hospital pad composed of 100% cotton in size 30 x 30cm, moistened with 40 ml of 70% ethanol. Bacteriological sampling was used to ensure fenestrated sterile field in the same site collection using Swab. The swab was transported to the laboratory in culture medium gel Stuart for identification. After testing, was performed antibiogram of isolates and sensitivity test in vitro using Kirby and Bauer method with modifications as described by Cooke (1988). For in vitro test used three disinfectants front seats of the isolated bacteria that are considered of great importance in the control of hospital infection. The result demonstrated that the largest colony-forming unit was compared to Staphylococcus sp, followed by Gram-positive bacillus, Staphylococcus aureus, Pseudomonas, Enterobacter cloacae, and Acinetobacter, predominantly Staphylococcus sp on all surfaces of the chair, with predominance in the left arm armchair coinciding with the arm of the patient who has an arteriovenous fistula (70%). Regarding sensitivity, Acinetobacter were sensitive to all antibiotics tested, whereas Pseudomonas were sensitive to sulfa / trimethoprim, ...
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Lacerda, Lilian Amaral [UNESP]. "Sistema para depleção de proteínas em Xanthomonas citri subsp. citri." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/134125.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Xanthomonas. citri subsp. citri (Xac) é o agente causal do cancro cítrico, uma das doenças mais graves que afetam plantas cítricas de importância comercial em todo o mundo. O controle eficaz para o cancro cítrico constitui na eliminação de plantas infectadas em programas rigorosos para conter a propagação da bactéria. No entanto, o relaxamento recente nas políticas para combater cancro cítrico em áreas como o estado de São Paulo - Brasil, está a contribuir para a propagação da doença. O conhecimento da biologia deste patógeno de plantas e também dos mecanismos envolvidos na interação planta-patógeno são de grande importância para apoiar o desenvolvimento de estratégias para lidar com o cancro cítrico. Um trabalho recente de nosso grupo demonstrou que os mutantes X. citri com disrupção do gene parB são comprometidos na segregação cromossômica e na capacidade de produzir os sintomas da doença na planta. No presente trabalho, nós estendemos nossas caracterizações, avaliando os efeitos da falta de parB em mutantes X. citri através de uma deleção limpa. ParB é uma proteína de ligação ao DNA e está envolvida no processo de segregação cromossômica através de interação com outras proteínas. Devido à dificuldade de deleção de genes envolvidos neste processo em X. citri, o nocaute de parB foi realizado numa variante abrigando um plasmídeo replicativo capaz de expressar cópias extras de ParB (ParB-TAP) sob o controle de para (promotor de arabinose). A viabilidade de X. citri parB nocaute (XacΔparB-pLAC2) foi avaliada em experimentos de curva de crescimento e em comparação com a estirpe do tipo selvagem, em condições de repressão ou de indução de parB-tap ligado a este promotor; O objetivo foi explorar as condições de depleção e superexpressão de ParB e seus efeitos através da caracterização do referido promotor. Em seguida, X. citri parB nocaute foi visualizado por microscopia de...
Xanthomonas citri ssp. citri (Xac) is the causal agent of citrus canker, one of the most severe diseases that affect citrus plants of commercial importance around the world. An effective control for citrus canker constitutes the elimination of infected plants in rigorous programs to contain the spread of the bacteria. However, recent relaxation in the policies to combat citrus canker in areas such as the state of São Paulo, Brazil, the major orange juice producer in the world, are contributing to the spread of the disease. Therefore, knowledge of the biology of this plant pathogen and also of the mechanisms involved in plant-pathogen interaction are of great importance to support the development of strategies to deal with citrus canker. Recent work from our group demonstrated that X. citri mutants disrupted for parB are compromised in chromosome segregation and are unable to produce disease symptoms in plant. In the present work, we extended our characterizations by evaluating the effects of a lack of ParB in X. citri mutants carrying a clean deletion of its coding DNA. ParB is a DNA binding protein and is involved in chromosome segregation process through interaction with other proteins. Because of difficult in genes deletion involved this process in X. citri, the parB gene was deleted in a X. citri variant harboring a replicative plasmid able to express extra copies of ParB (ParB-TAP) under the control of the para (arabinose promoter). The viability of the X. citri parB knockout was assessed in growth curve experiments and compared with the wild type strain under different conditions of parB-tap repression or induction. The aim was to explore the conditions depletion and overexpression of ParB and its effects through the characterization of para in X. citri Thus, the X. citri parB knockout was visualized under differential interference contrast (DIC) microscopy in order to detect morphological alterations normally expected from ...
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3

Lacerda, Lilian Amaral. "Sistema para depleção de proteínas em Xanthomonas citri subsp. citri /." Rio Claro, 2015. http://hdl.handle.net/11449/134125.

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Orientador: Henrique Ferreira
Banca: Alessandra Alves de Souza
Banca: Patricia Pasquali Parise Maltempi
Resumo: Xanthomonas. citri subsp. citri (Xac) é o agente causal do cancro cítrico, uma das doenças mais graves que afetam plantas cítricas de importância comercial em todo o mundo. O controle eficaz para o cancro cítrico constitui na eliminação de plantas infectadas em programas rigorosos para conter a propagação da bactéria. No entanto, o relaxamento recente nas políticas para combater cancro cítrico em áreas como o estado de São Paulo - Brasil, está a contribuir para a propagação da doença. O conhecimento da biologia deste patógeno de plantas e também dos mecanismos envolvidos na interação planta-patógeno são de grande importância para apoiar o desenvolvimento de estratégias para lidar com o cancro cítrico. Um trabalho recente de nosso grupo demonstrou que os mutantes X. citri com disrupção do gene parB são comprometidos na segregação cromossômica e na capacidade de produzir os sintomas da doença na planta. No presente trabalho, nós estendemos nossas caracterizações, avaliando os efeitos da falta de parB em mutantes X. citri através de uma deleção limpa. ParB é uma proteína de ligação ao DNA e está envolvida no processo de segregação cromossômica através de interação com outras proteínas. Devido à dificuldade de deleção de genes envolvidos neste processo em X. citri, o nocaute de parB foi realizado numa variante abrigando um plasmídeo replicativo capaz de expressar cópias extras de ParB (ParB-TAP) sob o controle de para (promotor de arabinose). A viabilidade de X. citri parB nocaute (XacΔparB-pLAC2) foi avaliada em experimentos de curva de crescimento e em comparação com a estirpe do tipo selvagem, em condições de repressão ou de indução de parB-tap ligado a este promotor; O objetivo foi explorar as condições de depleção e superexpressão de ParB e seus efeitos através da caracterização do referido promotor. Em seguida, X. citri parB nocaute foi visualizado por microscopia de...
Abstract: Xanthomonas citri ssp. citri (Xac) is the causal agent of citrus canker, one of the most severe diseases that affect citrus plants of commercial importance around the world. An effective control for citrus canker constitutes the elimination of infected plants in rigorous programs to contain the spread of the bacteria. However, recent relaxation in the policies to combat citrus canker in areas such as the state of São Paulo, Brazil, the major orange juice producer in the world, are contributing to the spread of the disease. Therefore, knowledge of the biology of this plant pathogen and also of the mechanisms involved in plant-pathogen interaction are of great importance to support the development of strategies to deal with citrus canker. Recent work from our group demonstrated that X. citri mutants disrupted for parB are compromised in chromosome segregation and are unable to produce disease symptoms in plant. In the present work, we extended our characterizations by evaluating the effects of a lack of ParB in X. citri mutants carrying a clean deletion of its coding DNA. ParB is a DNA binding protein and is involved in chromosome segregation process through interaction with other proteins. Because of difficult in genes deletion involved this process in X. citri, the parB gene was deleted in a X. citri variant harboring a replicative plasmid able to express extra copies of ParB (ParB-TAP) under the control of the para (arabinose promoter). The viability of the X. citri parB knockout was assessed in growth curve experiments and compared with the wild type strain under different conditions of parB-tap repression or induction. The aim was to explore the conditions depletion and overexpression of ParB and its effects through the characterization of para in X. citri Thus, the X. citri parB knockout was visualized under differential interference contrast (DIC) microscopy in order to detect morphological alterations normally expected from ...
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4

Ferreira, Natália Cristina. "Condições bacteriológicas da superfície das poltronas hematológicas par hemodiálise /." Botucatu, 2014. http://hdl.handle.net/11449/113878.

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Orientador: Ione Corrêa
Banca: Sílvia Maria Caldeira
Banca: Denise de Andrade
Resumo: Os estabelecimentos de saúde e as superfícies inanimadas que cercam o paciente guardam íntima relação com as infecções relacionadas a assistência e a saúde, sendo importantes focos de contato e de transmissão de agentes patogênicos e podem ser transferidos para as mais diversas superfícies através do contato direto e indireto. Existem inúmeras pesquisas que retratam que os artigos hospitalares podem se tornar veículos de disseminação, comprometendo a segurança do paciente. Diante desta problemática, este estudo objetivou-se em analisar as condições bacteriológicas de superficie de poltronas hematológicas para hemodiálise. Após aprovação do Comitê de Ética em pesquisa foi realizada a randomização das poltronas, duplo cego e iniciou a amostragem das quatro cadeira nos sete procedimentos em cinco locais diferentes na poltrona. O produto utilizado para higienização de rotina na unidade foi o álcool a 70% com compressa hospitalar composta de tecido 100% algodão em dimensão de 30 x 30 cm, umedecida com 40 ml de álcool 70%. Para amostragem bacteriológica utilizou-se campo estéril fenestrado para garantir coleta sempre no mesmo local utilizando Swab. O Swab foi transportado para o laboratório em meio de cultura gel Stuart para identificação. Após foi realizado teste de antibiograma das bactérias isoladas e teste de sensibilidade "in vitro" utilizando método de Kirby e Bauer com modificações conforme descrito por Corrêa (1988). Para o teste "in vitro" utilizou-se três desinfetantes frente as bactérias isoladas das poltronas que são consideradas de grande importância no controle de infecção hospitalar. O resultado demostrou que a maior unidade formadora de colônia foi em relação ao Staphilococcus sp, seguido de bacilus Gram positivo, Staphilococcus aureus, Pseudomonas, Enterobacter cloacae e Acinetobacter, com predominância do Staphilococcus sp em todas as ...
Abstract: Health facilities and inanimate surfaces surrounding the patient keep close relation with infections related to care and health, with important centers of contact and transmission of pathogens and can be transferred to many different surfaces through direct and indirect contact. There are numerous studies that show that the hospital items can become vehicles of dissemination, compromising patient safety. Faced with this problem, this study aimed to analyze the bacteriological conditions in the surface of hematologic arm for hemodialysis. After approval by the Research Ethics Committee of the randomization arm, double- blind trial was performed and started sampling the four chair the seven procedures in five different locations in the chair. The product used for routine cleaning of the unit was 70% alcohol with hospital pad composed of 100% cotton in size 30 x 30cm, moistened with 40 ml of 70% ethanol. Bacteriological sampling was used to ensure fenestrated sterile field in the same site collection using Swab. The swab was transported to the laboratory in culture medium gel Stuart for identification. After testing, was performed antibiogram of isolates and sensitivity test "in vitro" using Kirby and Bauer method with modifications as described by Cooke (1988). For "in vitro" test used three disinfectants front seats of the isolated bacteria that are considered of great importance in the control of hospital infection. The result demonstrated that the largest colony-forming unit was compared to Staphylococcus sp, followed by Gram-positive bacillus, Staphylococcus aureus, Pseudomonas, Enterobacter cloacae, and Acinetobacter, predominantly Staphylococcus sp on all surfaces of the chair, with predominance in the left arm armchair coinciding with the arm of the patient who has an arteriovenous fistula (70%). Regarding sensitivity, Acinetobacter were sensitive to all antibiotics tested, whereas Pseudomonas were sensitive to sulfa / trimethoprim, ...
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Meneghine, Aylan Kener [UNESP]. "Diversidade bacteriana em piscicultura neotropical." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/94875.

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A aquicultura é uma atividade que está em contínuo crescimento, desta forma os ecossistemas aquáticos utilizados para tal atividade acabam sofrendo alterações nas condições da água e na comunidade microbiana do local. No presente trabalho, compararam-se as comunidades bacterianas presentes em águas de dois viveiros de piscicultura, um utilizado como reservatório de água (V1) e o outro com condições de elevada carga de nutrientes (V4), os quais estão inseridos em um sistema sequencial com fluxo contínuo de água (seis viveiros). A escolha dos viveiros V1 e V4, foi baseada em estudos anteriores no qual foi evidenciado um alto grau de eutrofização neste local. As coletas de água foram realizadas em dois períodos distintos um na seca (Julho e Agosto/2012) e outro na chuva (Janeiro e Fevereiro/2012). A comunidade bacteriana foi avaliada através do sequenciamento do gene 16S rRNA. A população bacteriana variou significativamente entre os dois viveiros e segundo a estação anual, seca ou chuva. No viveiro 1 observou-se uma grande proporção de Proteobacterias, em ambos os períodos, entretanto houve mudanças nas classes deste grupo, a maioria β-Proteobacterias (chuvas) e uma proporção equivalente de β e α-Proteobacterias (seca). No viveiro 4, foi observada uma alta frequência de Cyanobacterias principalmente na estação de seca, aproximadamente 80% da população bacteriana do local, enquanto no período de chuvas este grupo foi menor. A comparação da população bacteriana dos dois viveiros estudados em épocas distintas mostrou que as bactérias nestes locais variam de acordo com o período hidrológico e estão intimamente ligadas com a concentração de nutrientes do local. Estas diferenças são importantes na participação dos ciclos biogeoquímicos, principalmente envolvendo os ciclos do nitrogênio e fósforo e a sanidade do sistema aquático.
The aquaculture is an activity that is continuous growing, in this way aquatic ecosystems used for such activity end up suffer changes in water conditions and microbial community local. In this study, we compared the bacterial communities present in waters of two fish farm ponds, a used as water reservoir (V1) and the other with conditions of high nutrient load (V4), which are inserted in a sequential system with continous water flow (six ponds). The choice of nurseries V1 and V4 was based on previous studies in which evidenced a high degree of eutrophication in this location. The water sampling were conducted in two distinct periods one in dry season (July and August/2012) and another in the rain (January and February/2012). The bacterial community was assessed by sequencing the 16S rRNA. The bacterial population varied significantly between the two ponds and according to season annual, dry or rain. In the pond 1 there was a large proportion of proteobacteria, in both periods, however there were changes in the classes of this group, most β-Proteobacteria (rain) and an equivalent proportion of β and α-proteobacteria (dry). In pond 4, we observed a high frequency of cyanobacteria mainly during the dry season, approximately 80% of the bacterial population of the place, while in the rainy season this group was lower. A comparison of the bacterial population of the two ponds studied at different times showed that the bacteria at these sites varies according to the period hydrological and are closely linked with nutrient concentration local. These differences are important in the participation in biogeochemical cycles, especially involving the cycles of nitrogen and phosphorus and health of the aquatic system.
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Gama, Nilce Maria Soares Queiroz [UNESP]. "Qualidade química e bacteriológica da água utilizada em granjas produtoras de ovos." Universidade Estadual Paulista (UNESP), 2005. http://hdl.handle.net/11449/104649.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Para as aves, a água é o nutriente essencial mais importante, sendo responsável pela maioria das funções do organismo. Em 2004 avaliou-se a qualidade da água utilizada em 20 granjas de postura comercial de Bastos/SP, Brasil, colhendo-se amostras dos poços, reservatórios e galpões. Nas amostras dos poços determinou-se a concentração de alumínio, cálcio, chumbo, cloro, cobre, enxofre, ferro, fósforo, magnésio, nitrito, potássio, selênio, sódio, sulfatos, zinco, pH e dureza. Nas amostras colhidas nos períodos de chuva e estiagem, realizaram-se as contagens de coliformes totais e fecais, estreptococos fecais, Pseudomonas aeruginosa e pesquisou-se Escherichia coli , Salmonella sp e Pasteurella multocida. A dureza da água de 35% das propriedades apresentou valores entre 60 - 110 mg CaCO3/L, em 45% o pH foi =6 e nenhuma apresentou pH >8. Entre as propriedades 50% apresentaram níveis de NO3-N =3 mg/L. Para todos os microrganismos pesquisados, o galpão de aves apresentou as maiores taxas de amostras positivas nos períodos de chuva e estiagem, ocorrendo a diminuição da % de isolamento no período de estiagem. A contagem bacteriana foi maior nas amostras de água do galpão, nos dois períodos de colheita. No reservatório, após a adição de cloro à água, o número de coliformes totais diminuiu e não houve detecção de coliformes fecais, estreptococos fecais e Pseudomonas aeruginosa. Em nenhuma das 440 amostras de água analisadas, nos períodos de chuva e estiagem, foram detectadas Salmonella sp e Pasteurella multocida.
Water is considered the most important essential nutrient, for chickens, being responsible for most of the organism functions. In 2004, the quality of the water used in 20 laying hens farms from Bastos/SP, Brazil, was tested, picking samples from wells, water tanks and laying hens house. It was determinated in the well-water samples the concentration of aluminum, calcium, lead, chlorine, copper, iron, phosphorus, magnesium, nitrate, nitrite, potassium, selenium, sodium sulfate, zinc, pH and strength. In the water samples, collected in the rainy and dry seasons, were counted the total coliforms, fecal coliforms, fecal streptococci, Pseudomonas aeruginosa and was done the research of Escherichia coli , Salmonella sp e Pasteurella multocida. The strengths values of 35% of the farms was between 60-110 mg CaCO3/L, in 45%, the pH was =6 and no farms presented pH >8. Among the farms 50% presented levels of NO3-N =3. For all microorganism researched, the laying hens house presented the higher taxes of positives samples during the rainy or dry seasons, occurring a reduction of isolation taxes in the dry season. Both periods of collection the number of total coliforms; fecal coliforms; streptococci and Pseudomonas aeruginosa was higher in the samples from the laying hens houses. In the water tank, after chlorine addiction to the water, the number of total and fecal coliforms decreased and there wasn't detection of fecal streptococci and Pseudomonas aeruginosa. In none of the 440 water samples analyzed, in the rainy nor in the dry seasons, Salmonella sp and Pasteurella multocida were detected.
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Sandonato, Beatriz Brabetz [UNESP]. "Peptídeos cíclicos hepatotóxicos: MALDI-TOF como uma ferramenta analítica." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/123921.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
As cianobactérias são bactérias fotossintetizantes que podem ser encontradas nos mais variados ambientes. Algumas espécies de cianobactérias produzem toxinas denominadas cianotoxinas. As cianotoxinas denominadas microcistinas (MCs) são heptapeptídeos cíclicos hepatotóxicos produzidas durante florações de cianobactérias. Sua detecção e quantificação em mananciais são de grande importância devido aos danos a saúde humana que essas microcistinas podem causar. Atualmente, a técnica MALDI-TOF-MS tem se mostrado uma eficiente ferramenta para a análise de microcistinas, e recentes estudos tem reportado seu uso na quantificação destas. Diante do exposto, o objetivo desta dissertação foi a avaliação de diferentes matrizes e diferentes métodos de preparo de amostra para MALDI-MS visando a detecção e quantificação das microcistinas. Com a finalidade de alcançar o melhor método de quantificação das microcistinas, nove matrizes para MALDI, onze métodos de preparo de amostra mais um método que foi adaptado por nosso grupo de pesquisa, o método Vacuum drying adaptado, e dois padrões comerciais de microcistinas, MC-LR e MC-RR, foram utilizados. A avaliação dos métodos de preparo de amostra foi realizada utilizando como matriz o ácido α-ciano-4-hidroxicinâmico (HCCA) e o peptídeo angiotensina I como padrão interno. Os métodos foram avaliados com relação aos seus coeficientes de variação (CV), suas cristalizações e espectros obtidos, utilizando a MC-RR como amostra. O método Vacuum drying adaptado apresentou os melhores resultados e sua curva analítica foi construída utilizando ambas as variantes de microcistinas. O limite de detecção (MLD) e o limite de quantificação (LDQ) também foram calculados. Cada curva da variante de microcistina apresentou excelente linearidade e os valores de r variaram entre 0,98-99, demonstrando que o método é adequado para a quantificação destas. Após as análises dos...
Cyanobacteria are photosynthetic bacteria that can be found in diverse environments. Some species of cyanobacteria produce toxins denominated cyanotoxins. The cyanotoxins called microcystins (MCs) are hepatotoxic cyclic heptapeptides produced during cyanobacterial blooms. Its detection and quantification in springs are of great importance due to damage to human health that these microcystins can cause. Currently, MALDI-TOF-MS technique has been shown to be an efficient tool for the analysis of microcystins, and recent studies have reported its use in the quantification of these. On the exposed, the aim of this thesis was to evaluate the different matrices and different methods of sample preparation for MALDI-MS aimed at the detection and quantification of microcystins. With the aim of achieving the best method of quantification of microcystins nine matrices for MALDI eleven methods of sample preparation over a method that was adapted by our research group, the adapted vacuum drying method, and two commercial standards of microcystins, MC-LR, and MC-RR, are used. The evaluation methods of sample preparation was performed using as matrix the α- cyano-4-hydroxycinnamic acid (CHCA) and the angiotensin I as internal standard. The methods were evaluated with respect to their coefficients of variation (CV), their crystallization and spectra obtained using the MC-RR as a sample. The adapted vacuum drying method showed the best results and their analytical curve was constructed using both variants of microcystins. The limit of detection (MDL) and the limit of quantification (LOQ) were also calculated. Each curve variant of microcystin showed excellent linearity and r values ranged from 0.98 to 99, showing that the method is suitable for quantifying these. After analysis of the methods of sample preparation, the nine matrices were evaluated with respect to CV, crystallization and spectra, using the MC-RR as a sample. The best results were obtained...
FAPESP: 2012/03663-8
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Meneghine, Aylan Kener. "Diversidade bacteriana em piscicultura neotropical /." Jaboticabal, 2013. http://hdl.handle.net/11449/94875.

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Orientador: Lucia Maria Carareto Alves
Banca: Lucia Helena Sipauba-Tavares
Banca: Rodrigo Matheus Pereira
Resumo: A aquicultura é uma atividade que está em contínuo crescimento, desta forma os ecossistemas aquáticos utilizados para tal atividade acabam sofrendo alterações nas condições da água e na comunidade microbiana do local. No presente trabalho, compararam-se as comunidades bacterianas presentes em águas de dois viveiros de piscicultura, um utilizado como reservatório de água (V1) e o outro com condições de elevada carga de nutrientes (V4), os quais estão inseridos em um sistema sequencial com fluxo contínuo de água (seis viveiros). A escolha dos viveiros V1 e V4, foi baseada em estudos anteriores no qual foi evidenciado um alto grau de eutrofização neste local. As coletas de água foram realizadas em dois períodos distintos um na seca (Julho e Agosto/2012) e outro na chuva (Janeiro e Fevereiro/2012). A comunidade bacteriana foi avaliada através do sequenciamento do gene 16S rRNA. A população bacteriana variou significativamente entre os dois viveiros e segundo a estação anual, seca ou chuva. No viveiro 1 observou-se uma grande proporção de Proteobacterias, em ambos os períodos, entretanto houve mudanças nas classes deste grupo, a maioria β-Proteobacterias (chuvas) e uma proporção equivalente de β e α-Proteobacterias (seca). No viveiro 4, foi observada uma alta frequência de Cyanobacterias principalmente na estação de seca, aproximadamente 80% da população bacteriana do local, enquanto no período de chuvas este grupo foi menor. A comparação da população bacteriana dos dois viveiros estudados em épocas distintas mostrou que as bactérias nestes locais variam de acordo com o período hidrológico e estão intimamente ligadas com a concentração de nutrientes do local. Estas diferenças são importantes na participação dos ciclos biogeoquímicos, principalmente envolvendo os ciclos do nitrogênio e fósforo e a sanidade do sistema aquático.
Abstract: The aquaculture is an activity that is continuous growing, in this way aquatic ecosystems used for such activity end up suffer changes in water conditions and microbial community local. In this study, we compared the bacterial communities present in waters of two fish farm ponds, a used as water reservoir (V1) and the other with conditions of high nutrient load (V4), which are inserted in a sequential system with continous water flow (six ponds). The choice of nurseries V1 and V4 was based on previous studies in which evidenced a high degree of eutrophication in this location. The water sampling were conducted in two distinct periods one in dry season (July and August/2012) and another in the rain (January and February/2012). The bacterial community was assessed by sequencing the 16S rRNA. The bacterial population varied significantly between the two ponds and according to season annual, dry or rain. In the pond 1 there was a large proportion of proteobacteria, in both periods, however there were changes in the classes of this group, most β-Proteobacteria (rain) and an equivalent proportion of β and α-proteobacteria (dry). In pond 4, we observed a high frequency of cyanobacteria mainly during the dry season, approximately 80% of the bacterial population of the place, while in the rainy season this group was lower. A comparison of the bacterial population of the two ponds studied at different times showed that the bacteria at these sites varies according to the period hydrological and are closely linked with nutrient concentration local. These differences are important in the participation in biogeochemical cycles, especially involving the cycles of nitrogen and phosphorus and health of the aquatic system.
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Sandonato, Beatriz Brabetz. "Peptídeos cíclicos hepatotóxicos : MALDI-TOF como uma ferramenta analítica /." Rio Claro, 2014. http://hdl.handle.net/11449/123921.

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Orientador: Humberto Márcio Santos Milagre
Banca: Paulo José Samenho Moran
Banca: José Augusto Rosário Rodrigues
Resumo: As cianobactérias são bactérias fotossintetizantes que podem ser encontradas nos mais variados ambientes. Algumas espécies de cianobactérias produzem toxinas denominadas cianotoxinas. As cianotoxinas denominadas microcistinas (MCs) são heptapeptídeos cíclicos hepatotóxicos produzidas durante florações de cianobactérias. Sua detecção e quantificação em mananciais são de grande importância devido aos danos a saúde humana que essas microcistinas podem causar. Atualmente, a técnica MALDI-TOF-MS tem se mostrado uma eficiente ferramenta para a análise de microcistinas, e recentes estudos tem reportado seu uso na quantificação destas. Diante do exposto, o objetivo desta dissertação foi a avaliação de diferentes matrizes e diferentes métodos de preparo de amostra para MALDI-MS visando a detecção e quantificação das microcistinas. Com a finalidade de alcançar o melhor método de quantificação das microcistinas, nove matrizes para MALDI, onze métodos de preparo de amostra mais um método que foi adaptado por nosso grupo de pesquisa, o método Vacuum drying adaptado, e dois padrões comerciais de microcistinas, MC-LR e MC-RR, foram utilizados. A avaliação dos métodos de preparo de amostra foi realizada utilizando como matriz o ácido α-ciano-4-hidroxicinâmico (HCCA) e o peptídeo angiotensina I como padrão interno. Os métodos foram avaliados com relação aos seus coeficientes de variação (CV), suas cristalizações e espectros obtidos, utilizando a MC-RR como amostra. O método Vacuum drying adaptado apresentou os melhores resultados e sua curva analítica foi construída utilizando ambas as variantes de microcistinas. O limite de detecção (MLD) e o limite de quantificação (LDQ) também foram calculados. Cada curva da variante de microcistina apresentou excelente linearidade e os valores de r variaram entre 0,98-99, demonstrando que o método é adequado para a quantificação destas. Após as análises dos...
Abstract: Cyanobacteria are photosynthetic bacteria that can be found in diverse environments. Some species of cyanobacteria produce toxins denominated cyanotoxins. The cyanotoxins called microcystins (MCs) are hepatotoxic cyclic heptapeptides produced during cyanobacterial blooms. Its detection and quantification in springs are of great importance due to damage to human health that these microcystins can cause. Currently, MALDI-TOF-MS technique has been shown to be an efficient tool for the analysis of microcystins, and recent studies have reported its use in the quantification of these. On the exposed, the aim of this thesis was to evaluate the different matrices and different methods of sample preparation for MALDI-MS aimed at the detection and quantification of microcystins. With the aim of achieving the best method of quantification of microcystins nine matrices for MALDI eleven methods of sample preparation over a method that was adapted by our research group, the adapted vacuum drying method, and two commercial standards of microcystins, MC-LR, and MC-RR, are used. The evaluation methods of sample preparation was performed using as matrix the α- cyano-4-hydroxycinnamic acid (CHCA) and the angiotensin I as internal standard. The methods were evaluated with respect to their coefficients of variation (CV), their crystallization and spectra obtained using the MC-RR as a sample. The adapted vacuum drying method showed the best results and their analytical curve was constructed using both variants of microcystins. The limit of detection (MDL) and the limit of quantification (LOQ) were also calculated. Each curve variant of microcystin showed excellent linearity and r values ranged from 0.98 to 99, showing that the method is suitable for quantifying these. After analysis of the methods of sample preparation, the nine matrices were evaluated with respect to CV, crystallization and spectra, using the MC-RR as a sample. The best results were obtained...
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Morgan, J. H. "Bacteriology of calf diarrhoea with special reference to Campylobacter." Thesis, University of Reading, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.254444.

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Books on the topic "Bacteriology"

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Woodford, Neil, and Alan Johnson. Molecular Bacteriology. New Jersey: Humana Press, 1998. http://dx.doi.org/10.1385/0896034984.

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Bishop-Lilly, Kimberly A., ed. Diagnostic Bacteriology. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7037-7.

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Whitman, William B., ed. Systematic Bacteriology. New York, NY: Springer New York, 2009. http://dx.doi.org/10.1007/978-0-387-68489-5.

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Ciancio, Aurelio. Invertebrate Bacteriology. Dordrecht: Springer Netherlands, 2016. http://dx.doi.org/10.1007/978-94-024-0884-3.

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Sleigh, J. D. Medical bacteriology. Edinburgh: Churchill Livinstone, 1992.

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1937-, Williams S. T., Bergey D. H. 1860-1937, Holt John G, and Sharpe M. Elisabeth, eds. Bergey's manual of systematic bacteriology. Baltimore: Williams & Wilkins, 1989.

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Sleigh, J. Douglas. Notes on medical bacteriology. 2nd ed. Edinburgh: Churchill Livingstone, 1986.

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Sleigh, J. Douglas. Notes on medical bacteriology. 4th ed. Edinburgh: Churchill Livingstone, 1994.

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Howard, Jenny, and David M. Whitcombe. Diagnostic Bacteriology Protocols. New Jersey: Humana Press, 1995. http://dx.doi.org/10.1385/0896032973.

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Louise, O'Connor. Diagnostic Bacteriology Protocols. New Jersey: Humana Press, 2006. http://dx.doi.org/10.1385/1597451436.

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Book chapters on the topic "Bacteriology"

1

McGrew, Roderick E. "Bacteriology." In Encyclopedia of Medical History, 25–46. London: Palgrave Macmillan UK, 1985. http://dx.doi.org/10.1007/978-1-349-05429-9_2.

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Parish, Lawrence Charles, Joseph A. Witkowski, and John Thorne Crissey. "Bacteriology." In The Decubitus Ulcer in Clinical Practice, 71–74. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60509-3_9.

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Boulahbal, F. "Bacteriology." In Tuberculosis of the Bones and Joints, 23–27. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-61358-6_5.

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Lloyd, Ricardo V. "Bacteriology." In Pathology: Historical and Contemporary Aspects, 173–79. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-39554-3_17.

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Roberts, Glenn D., and Gregory P. Thompson. "Bacteriology and Bacteriologic Diagnosis of Tuberculosis." In Tuberculosis, 51–61. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4613-8321-5_4.

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Roberts, Glenn D. "Bacteriology and Bacteriologic Diagnosis of Tuberculosis." In Tuberculosis, 23–31. New York, NY: Springer New York, 1988. http://dx.doi.org/10.1007/978-1-4684-0305-3_3.

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Nasmyth, D. G. "Bacteriology (I)." In Topics in Colorectal Disease, 131–32. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76281-9_17.

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Hill, M. J., and F. Fernandez. "Bacteriology (II)." In Topics in Colorectal Disease, 132–34. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76281-9_18.

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Allen, Stephen D., Christopher L. Emery, and Jean A. Siders. "Anaerobic Bacteriology." In Manual of Commercial Methods in Clinical Microbiology, 50–83. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817961.ch4.

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Gradmann, Christoph. "Medical Bacteriology." In The Routledge History of Disease, 378–401. Abingdon, Oxon ; New York, NY : Routledge, 2016. | Series: The Routledge histories: Routledge, 2016. http://dx.doi.org/10.4324/9781315543420-21.

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Conference papers on the topic "Bacteriology"

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Hernández, Wilder, Sandra Bibiana Avendaño Avendaño, and Luis Gabriel Gutierrez. "Musculoskeletal Risk Level among Health Professionals of a Health Entity." In 13th International Conference on Applied Human Factors and Ergonomics (AHFE 2022). AHFE International, 2022. http://dx.doi.org/10.54941/ahfe1002618.

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The level of musculoskeletal risk in upper limbs was determined in five areas where health professionals of a health entity in Bogotá-Colombia practice. The essential duties in the Medicine, Bacteriology, Dentistry, Physiotherapy, and Nursing spaces were selected through interviews with employees; the RULA approach was then used to establish the most critical components and the final score. At the level of the neck, shoulders, and wrists, forced postures were the variable that increased the score in areas such as dentistry, Bacteriology, and Physiotherapy. In almost all areas, load handling and movements with high frequency were evidenced. The above findings guide the improvement actions, and the consideration of other factors to be reviewed is recommended.
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Lim, H., SM Lee, J. Yim, C. Yoo, YW Kim, SK Han, Y. Shim, and S. Yang. "Bacteriology of Obstructive Pneumonia Caused by Lung Cancer." In American Thoracic Society 2009 International Conference, May 15-20, 2009 • San Diego, California. American Thoracic Society, 2009. http://dx.doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a1726.

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Hadid, Walid, Khalid Mohammad, Basel Ericsoussi, Dean E. Schraufnagel, John W. Christman, and Ruxana T. Sadikot. "Epidemiology And Bacteriology Of Adult Non-CF Bronchiectasis." In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a3176.

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Atikov, A. S. "THE ASSOCIATION OF LIVER PATHOLOGIES WITH THE CONTAMINATION OF THE GASTROINTESTINAL TRACT WITH H.PYLORI." In SPbVetScience. FSBEI HE St. Petersburg SUVM, 2023. http://dx.doi.org/10.52419/3006-2023-11-9-16.

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Currently, one of the most promising areas in bacteriology is the study of the bacterium Helicobacter pylori. New studies show a link between H. pylori and some liver diseases, but their exact mechanisms and connections are still not fully understood. Despite this, studies of H. pylori and its association with liver diseases remain relevant to this day. This article presents an analysis of the association of H. Pylori with liver diseases.
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Chen, Wei-Chih, Desiree Schumann, Michael Tamm, and Daiana Stolz. "Sputum rheology and bacteriology in stable and exacerbated chronic obstructive pulmonary disease." In ERS International Congress 2020 abstracts. European Respiratory Society, 2020. http://dx.doi.org/10.1183/13993003.congress-2020.3281.

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Davies, Robert H., Peter J. Heath, Sue M. Coxon, and Robin A. Sayers. "Comparison of two commercial ELISA kits and bacteriology for Salmonella monitoring in pig herds." In Second International Symposium on Epidemiology and Control of Salmonella in Pork. Iowa State University, Digital Press, 2003. http://dx.doi.org/10.31274/safepork-180809-461.

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Nollet, Nathalie, K. Huysmans, Dominiek Maes, K. Houf, and H. Imberechts. "Correlation between bacteriology of lymph nodes and serology for Salmonella diagnosis in slaughter pigs." In Second International Symposium on Epidemiology and Control of Salmonella in Pork. Iowa State University, Digital Press, 2003. http://dx.doi.org/10.31274/safepork-180809-465.

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Çiftci, Fatma, Deniz Dogan Mulazımoglu, Serhat Erol, Aydin Ciledag, and Akin Kaya. "Effect of sputum bacteriology on the prognosis of patients with acute exacerbations of bronchiectasis." In ERS International Congress 2017 abstracts. European Respiratory Society, 2017. http://dx.doi.org/10.1183/1393003.congress-2017.pa2099.

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White, Heath D., Bobbie Ann White, Juhee Song, Robert Fader, Pedro Quiroga, and Alejandro C. Arroliga. "Thoracic Empyema: A Nine-year Single Institution Review Of Bacteriology, Case Characteristics, And Mortality." In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a6163.

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Ebihara, Satoru, Takae Ebihara, Shinsuke Yamanda, and Masahiro Kohzuki. "Bacteriology Of Aspiration Pneumonia Due To Delayed Triggering Of The Swallowing Reflex In Elderly Patients." In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a6176.

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Reports on the topic "Bacteriology"

1

Granigan, Marion. Travel grant program for the IX International Congresses of Mycology and Bacteriology -- Final report. Office of Scientific and Technical Information (OSTI), May 2000. http://dx.doi.org/10.2172/809201.

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Sutipatanasomboon, Arpaporn. Petri Dishes with Agar: How to Make Agar Plates. ConductScience, June 2022. http://dx.doi.org/10.55157/cs20220627.

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Petri dishes and agar form the foundation of the culture plate technique pioneered by Robert Koch, facilitating the cultivation and study of microorganisms. Agar, derived from red seaweed, solidifies into a transparent medium for microbial growth. Agar plates are classified as nonselective, allowing general growth, and selective, inhibiting specific microbes. Petri dishes, with a dish and lid, provide containers for agar, supporting microbial growth. This technique has revolutionized microbiological research, enabling advances in fields like bacteriology, mycology, infectious diseases, and biotechnology.
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Eldar, Avigdor, and Donald L. Evans. Streptococcus iniae Infections in Trout and Tilapia: Host-Pathogen Interactions, the Immune Response Toward the Pathogen and Vaccine Formulation. United States Department of Agriculture, December 2000. http://dx.doi.org/10.32747/2000.7575286.bard.

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In Israel and in the U.S., Streptococcus iniae is responsible for considerable losses in various fish species. Poor understanding of its virulence factors and limited know-how-to of vaccine formulation and administration are the main reasons for the limited efficacy of vaccines. Our strategy was that in order to Improve control measures, both aspects should be equally addressed. Our proposal included the following objectives: (i) construction of host-pathogen interaction models; (ii) characterization of virulence factors and immunodominant antigens, with assessment of their relative importance in terms of protection and (iii) genetic identification of virulence factors and genes, with evaluation of the protective effect of recombinant proteins. We have shown that two different serotypes are involved. Their capsular polysaccharides (CPS) were characterized, and proved to play an important role in immune evasion and in other consequences of the infection. This is an innovative finding in fish bacteriology and resembles what, in other fields, has become apparent in the recent years: S. iniae alters surface antigens. By so doing, the pathogen escapes immune destruction. Immunological assays (agar-gel immunodiffusion and antibody titers) confirmed that only limited cross recognition between the two types occurs and that capsular polysaccharides are immunodominant. Vaccination with purified CPS (as an acellular vaccine) results in protection. In vitro and ex-vivo models have allowed us to unravel additional insights of the host-pathogen interactions. S. iniae 173 (type II) produced DNA fragmentation of TMB-8 cells characteristic of cellular necrosis; the same isolate also prevented the development of apoptosis in NCC. This was determined by finding reduced expression of phosphotidylserine (PS) on the outer membrane leaflet of NCC. NCC treated with this isolate had very high levels of cellular necrosis compared to all other isolates. This cellular pathology was confirmed by observing reduced DNA laddering in these same treated cells. Transmission EM also showed characteristic necrotic cellular changes in treated cells. To determine if the (in vitro) PCD/apoptosis protective effects of #173 correlated with any in vivo activity, tilapia were injected IV with #173 and #164 (an Israeli type I strain). Following injection, purified NCC were tested (in vitro) for cytotoxicity against HL-60 target cells. Four significant observations were made : (i) fish injected with #173 had 100-400% increased cytotoxicity compared to #164 (ii) in vivo activation occurred within 5 minutes of injection; (iii) activation occurred only within the peripheral blood compartment; and (iv) the isolate that protected NCC from apoptosis in vitro caused in vivo activation of cytotoxicity. The levels of in vivo cytotoxicity responses are associated with certain pathogens (pathogen associated molecular patterns/PAMP) and with the tissue of origin of NCC. NCC from different tissue (i.e. PBL, anterior kidney, spleen) exist in different states of differentiation. Random amplified polymorphic DNA (RAPD) analysis revealed the "adaptation" of the bacterium to the vaccinated environment, suggesting a "Darwinian-like" evolution of any bacterium. Due to the selective pressure which has occurred in the vaccinated environment, type II strains, able to evade the protective response elicited by the vaccine, have evolved from type I strains. The increased virulence through the appropriation of a novel antigenic composition conforms with pathogenic mechanisms described for other streptococci. Vaccine efficacy was improved: water-in-oil formulations were found effective in inducing protection that lasted for a period of (at least) 6 months. Protection was evaluated by functional tests - the protective effect, and immunological parameters - elicitation of T- and B-cells proliferation. Vaccinated fish were found to be resistant to the disease for (at least) six months; protection was accompanied by activation of the cellular and the humoral branches.
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