Relevant bibliographies by topics / Bacterial isolates

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Academic literature on the topic 'Bacterial isolates'

Author: Grafiati
Published: 4 June 2021
Last updated: 18 May 2024

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Journal articles on the topic "Bacterial isolates"

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Pattani, Vivek B. "Characterization of Plant Growth-Promoting Activity of Bacteria Isolated from Forest and Coastal Regions of Saurashtra, Gujarat, India." Bioscience Biotechnology Research Communications 15, no. 1 (March 25, 2022): 144–51. http://dx.doi.org/10.21786/bbrc//15.1.22.

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The haphazard application of chemical fertilizers and pesticides causes tremendous damage to ecosystems and all biota. One of the most effective ways to tackle the threat is to use biofertilizer. Plant growth promoting bacteria (PGPB) are an important bacterial source for microbial fertilizers that can boost agricultural yields by encouraging plant growth. Bacterial isolates isolated from Saurashtra region, Gujarat, India were analysed for their capability to solubilize inorganic 'P' from tri calcium phosphate and production of indole acetic acid (IAA) quantitatively by bacterial. Production of ammonia, siderophore and hydrogen cyanide (HCN) by selected bacteria isolates was analysed. Biochemical characterization of selected bacterial isolates was done using Vitek 2 Compact system. Isolate GFS15C2 showed highest amount of phosphate solubilization, followed by isolate GFS07C1 and GFS01C1. Bacterial isolate GFS15C2 produced highest amount of IAA. All bacterial isolates were able produce ammonia. Eight bacteria isolates were be to produce HCN. Siderophore was produced by 14 bacterial isolates. In biochemical characterization all the bacterial isolates were able to use D-glucose. Based on biochemical characters clustering of bacteria isolates was done using Paleontological statistics software package for education and data analysis(PAST). Using cluster analysis by euclidean distance method based on biochemical characterization isolates GFS16C2 & SCS12C3 was found to have distinct characters than other isolates. The present study attempts to characterize PGPB which could be harnessed to improve plant growth. Several phosphate solubilizers and IAA producers also showed production of siderophores and HCN which suggests that these organisms do possess biocontrol ability. These PGPB microbial inoculants can be utilized to improve agricultural systems or as an alternate means of environmentally friendly plant disease biocontrol.
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RINA, NURFITRIANI, NI PUTU RATNA AYU KRISHANTI, ALINA AKHDIYA, and ARIS TRI WAHYUDI. "Penapisan Bakteri Filosfer Penghasil Senyawa Bioaktif Anti Xanthomonas oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri pada Padi." Jurnal Sumberdaya Hayati 2, no. 1 (November 14, 2016): 19–24. http://dx.doi.org/10.29244/jsdh.2.1.19-24.

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Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the important diseases in rice crops in Indonesia. The disease is difficult to be controlled since it attacks the rice plant at different growth stages such as tillering, flowering and ripening. One of the alternatives that could be used to control the disease is by using phyllosphere bacteria as the biocontrol agents. This study aims to isolate, characterize and screen the rice phyllosphere bacteria producing bioactive compounds against Xoo. Phyllosphere bacteria isolated from healthy leaves of rice var. Ciherang by using 4 different media obtained 285 bacterial isolates which were consisted of the 65 isolates of King’s B agar, 86 isolates of Nutrient agar, 81 isolates of Luria-Bertani agar, and 53 isolates of Trypticase Soy agar media. Antagonist test using double layer method showed 58 isolates of phyllosphere bacteria produced bioactive compounds that inhibited the growth of Xoo. Pathogenicity test agaist rice leaf revealed 18 bacterial isolates did not perform their potencies as pathogenic bacteria. Among the 18 non-phytopathogenic bacterial isolates, 14 isolates belong to Gram-positive bacteria and 4 isolates belong to Gram-negative bacteria. Five isolates among Gram positive bacteria were predicted as Bacillus genera.
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Abdullah Al-Kubaisy, Abdullah Kamel, and Huriya Hussein AlJuboori. "Evaluation of Inhibition Efficiency of Some Bacteria Isolated from Greenhouse Soils on the Growth of the Pathogenic Fungus Sclerotinia sclerotiorum that Causes White Rot Disease on Vegetables in the Laboratory." Arab Journal for Plant Protection 40, no. 2 (2022): 140–47. http://dx.doi.org/10.22268/ajpp-40.2.140147.

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Al-Kubaisy, A.K.A. and H.H. Al-Juboor. 2022. Evaluation of Inhibition Efficiency of Some Bacteria Isolated from Greenhouse Soils on the Growth of the Pathogenic Fungus Sclerotinia sclerotiorum that Causes White Rot Disease on Vegetables in the Laboratory. Arab Journal of Plant Protection, 40(2): 140-147. https://doi.org/10.22268/AJPP-40.2.140147 This study aimed to isolate beneficial bacteria from the soil of plastic houses planted with eggplant and cucumber at different locations of Baghdad Governorate and characterize them molecularly in addition to determining their antagonistic ability to inhibit six isolates of the pathogenic fungus Sclerotinia sclerotiorum, the causal agent of white rot disease. The isolation results showed that 18 different bacterial isolates were obtained from several fields in Baghdad governorate. Bacterial isolates showed antagonistic ability towards six isolates of the pathogenic fungus S. sclerotiorum (ScE1, ScE2, ScE3, ScE4, ScC1 and ScC2), and the inhibition rate ranged between 84.25 and 93.75%. The two bacterial isolates BE1 and BE6 excelled in plastic houses grown with eggplant plants, and the inhibition rate of the fungal pathogen reached 93.75%. Whereas, the bacterial isolate BC9 isolated from soils planted with cucumber plants achieved the highest inhibition rate of all fungal isolates, except isolate ScE1, which reached 84.25%. Bacterial isolates were identified molecularly and they were registered in the GenBank under accession numbers MZ436922, MZ436923, MZ436921 and MZ436920 for the isolates of Alcaligenes faecalis, Bacillus amyloliquefaciens, Bacillus subtilis and Pseudomonas aeruginosa, respectively. Keywords: Sclerotinia sclerotiorum, biological control, molecular identification, eggplant
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Febriani, Heni, Bernadeta Octavia, and Enny Zulaika. "PHENETIC DIVERSITY OF CELLULASE-PRODUCING BACTERIA FROM WANA TIRTA KULON PROGO MANGROVE FOREST." Indonesian Journal of Bioscience (IJOBI) 1, no. 1 (December 26, 2023): 1–14. http://dx.doi.org/10.21831/ijobi.v1i1.114.

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Mangrove is an ecosystem that have important value for the environment and are a source of cellulase-producing microorganism biomass. The purpose of this study was to determine the characteristics and types, as well as the highest cellulase enzyme activity from cellulase-producing bacteria found in Wana Tirta Mangrove Forest, Jangkaran, Kulon Progo. This research is a descriptive-exploratory research. Sampling in the form of litter, mud and water was carried out in the Wana Tirta Kulon Mangrove Forest which was divided into 3 plots. This sample is then isolated on selective media in the form of Carboxymethyl cellulose (CMC) In order to grow cellulolytic bacteria. The bacteria obtained are then purified and phenetic characterization. The data obtained were used to classify the bacterial isolates using MVSP identification software (Multivariate Statistical Package) 3.1 with UPGMA clustering algorithm (Unweight Pair Group Method With Arithmatic Averages) then the result is presented in the form of a dendogram. Result Research shows as many as 17 isolates of cellulolytic bacteria were obtained. There were 4 bacterial isolates from litter samples, 1 bacterial isolate from water samples and 12 bacterial isolates from mud samples. After being made in the form of a dendogram, 19 bacterial clusters were obtained. A total of 6 bacterial isolates had a similarity index of ≥ 72% against Bacillus pumilus namely isolates S1A 2, S2A, AL 3, AP 3, AP 6 and AP 17, 4 bacterial isolates have a similarity index of ≥ 70% to Bacillus stearothermophyllus namely AP 22, AP 24, AP 25, and AP 26 isolates. As well as 7 bacterial isolates have a similarity index of ≥ 77% against Streptomyces Sp. namely isolates S1A 1, S3B, AP 8, AP 9, AP 14, AP 20 and AP 27.The highest cellulase enzyme activity occurred in AP 14 bacterial isolate of 286.72 U / ml.
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Nurikhsanti, Mikiyal, Lalu Zulkifli, Dewa Ayu Citra Rasmi, and Prapti Sedijani. "Antagonistic Test of Bacteria Producing Siderophore and Protease Enzymes from The Rhizosfer of Peanut Plants on The Growth of Pathogenic Fungus Colletotrichum gloeosporioides." Jurnal Biologi Tropis 24, no. 1 (January 17, 2024): 100–108. http://dx.doi.org/10.29303/jbt.v24i1.6459.

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This study aims to determine the inhibitory effect of bacterial isolates producing siderophores and protease enzymes on the growth of the pathogenic fungus Colletotrichum gloeosporioides. The initial stage of research begins with the isolation of pathogenic bacteria and fungi, and is followed by testing the production of siderophores and protease enzymes. Bacteria were isolated from the rhizosphere of peanut plants, while pathogenic fungi were isolated from large chili fruit infected with anthracnose disease taken from Jelantik Village, Central Lombok Regency. Characterization of isolates for siderophore production used the Arnow method, while the protease enzyme production test used SKIM Milk Agar media. Next, the inhibition test of bacterial isolates against pathogenic fungi was carried out using the dual culture method. Characterization of potential isolates was carried out by observing bacterial colony morphology, gram staining and biochemical tests. The results of the siderophore production test showed that there was one isolate capable of producing siderophores with the isolate code RKT2. Meanwhile, the protease enzyme production test showed that all bacterial isolates produced protease enzymes, where isolate RKT9 had the highest Proteolytic Index, namely 1.57. The two isolates showed different inhibitory test results, namely isolate RKT2 had high inhibition, while RKT9 had low inhibition. The results of the research showed that a bacterial isolate (RKT2) from the rhizosphere of peanut plants was able to inhibit the growth of the pathogenic fungus Colletotrichum gloeosporioides in the high category.
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Nurhasnah, Nurhasnah, Media Roza, Milya Sari, and Kencanawati Kencanawati. "Antimicrobial Potential Of Endophyte Bacteria In Angsana Plants (Pterocarpus indicus Willd)." Bioscience 7, no. 1 (March 31, 2023): 48. http://dx.doi.org/10.24036/0202371121349-0-00.

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The discovery of antimicrobial active compounds is one of the pressing things in the world of medicine and the pharmaceutical industry, due to the increasing and widespread resistance of pathogenic bacteria to existing antimicrobials. Angsana plant (Pterocarpus Indicus Willd) has been shown to have efficacy as a drug, so it has the potential to be used as a source of natural antibiotics. This study aims to find out the types of endophytes in various parts of the Angsana plant (Pterocarpus indicus Willd) through macroscopic and microscopic identification and find out the activity of antimicrobial compound-producing endophye bacteria in Angsana plants. Bacterial purification techniques use streak plate and spread plate methods. Antimicrobial activity tests are carried out using diffusion methods by means of point inoculation. The results showed 29 isolates of endophye bacteria isolated from the Angsana plant (Pterocarpus indicus Willd). From the roots as many as 11 isolates, stems as many as 12 isolates, and leaves as many as 6 isolates. The result of Gram staining of Angsana plant endophyte bacteria, obtained 22 bacterial isolates including Gram positive and 7 is Gram negative. There were 21 bacil-shaped endophyte bacterial isolates, 7 coccus-shaped isolates and 1 coccobacil-shaped isolate. Isolate the endophyte bacteria of Angsana plants that have the potential to produce antimicrobial compounds as many as 20 isolates. Isolates of Angsana plant endophyte bacteria form a bland zone in S. aureus (10 isolates), E. coli (17 isolates). Endophyte bacterial isolates that are able to inhibit the growth of bacteria S. aureus and E. coli are 7 isolates
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Hermanu Triwidodo and Listihani Listihani. "The Isolation, Selection and Determination of Endophytic Bacteria from Bamboo, Gamal, Tulsi, and Alamanda." SEAS (Sustainable Environment Agricultural Science) 5, no. 2 (November 2, 2021): 151–62. http://dx.doi.org/10.22225/seas.5.2.4068.151-162.

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Endophytic bacteria have many benefits, including increasing plant growth by producing phytohormones, increasing the production of mineral absorption, nitrogen fixation, reducing damage due to weather changes and increasing plant resistance to disease. Based on the above, it is necessary to select endophytic bacteria from various plants to be used as biocontrol agents. This study aims to obtain endophytic bacterial isolates that have the potential as biocontrol agents and plant growth supporters from bamboo shoots, Gamal, Tulsi, Lotus, and Alamanda. This research method includes sampling, endophytic bacteria isolation, hypersensitive, hemolysis, phosphate solvent, chitinolytic, proteolytic, and antagonist tests. Isolation of endophytic bacteria in 5 plants using 22 plant parts had a diversity of isolates. The isolated plant parts produced 1 to 7 isolates that had different morphology. The total isolates obtained were 59 isolates. In antagonistic observations, there was one isolate of endophytic bacteria that showed a clear zone when tested together with S. rolfsii, namely the isolate with code A24 from allamanda flower. From the data obtained, it is known that the endophytic bacterial isolates had an effect on inhibiting the growth of the pathogenic fungus S. roflsii, the endophytic bacterial isolates Consortium, A21 and the endophytic bacterial isolates A22 had no incidence of disease, while the bacterial isolates T00 (Bx) with an average disease incidence of 40% and 30% disease intensity. Meanwhile isolates A23, A24 and A25 had an average disease incidence ranging from 13.3%-26.6%, while controls had the highest disease incidence, namely 53.3% and disease intensity 66.6%.
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De Fretes, Charlie Ester. "ISOLASI DAN KARAKTERISASI KEMAMPUAN BAKTERI ENDOFIT SORGUM MANIS FS501 SEBAGAI PENDUKUNG PERTUMBUHAN TANAMAN." EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi 5, no. 2 (December 31, 2020): 49–52. http://dx.doi.org/10.33019/ekotonia.v5i2.2107.

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This study aims to isolate endophytic bacteria of sweet sorghum plants and characterize their ability as plant growth promoting. The characters tested in this study were the ability of endophytic bacteria in N fixation, phosphate dissolving, and IAA production to be developed as biological fertilizer agents. Twenty-four isolates were isolated from the roots, stems and shoots of sweet sorghum. The results of bacterial DNA fingerprint screening showed that 11 groups of endophytic bacteria had different fingerprints. Isolates capable of N fixation were grown on LGI media and showed a change in the color of the medium. The nifH gene detection is also carried out to determine endophytic diazotrophic bacteria. Isolate bacteria that can dissolve inorganic phosphate were tested using Pikovskaya media. Testing the ability of isolates to produce IAA was carried out by adding Salkowski's reagent to the bacterial culture and measured quantitatively with a λ 530 nm spectrophotometer. The results showed that two endophytic bacterial isolates proved to be diazotrophic and three isolates were able to dissolve phosphate, while one isolate was able to produce IAA. PA2 isolate showed ability in all the characters tested, namely N fixation, phosphate solvent and IAA producer.
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Talip Abd-alla, Mays, Mohsen Hashim Risan, and Athraa H. Muhsin. "Microbial Contamination and Identification of Bacterial for Mobiles Phones in Iraq." Al-Kufa University Journal for Biology 7, no. 2 (August 30, 2015): 50–56. http://dx.doi.org/10.36320/ajb/v7.i2.8017.

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This study was conducted to isolate and identify bacteria contaminants on a mobile phone. The samples were collected randomly from 20 mobile phones. This study was conducted between October to December, 2016 at College of Biotechnology, Al-Nahrain University. The isolated colonies were then sub cultured in nutrient agar and slants in order to obtain pure culture of all the six colonies. Six genera of bacteria were identified from positive cultures. In all, 20 swab samples of mobile phone were randomly examined, 19 bacterial isolates were identified from mobile phones were found contaminated with microbiota. The highest prevalence of Staphylococcus aureus was observed in mobile phones. The research findings indicated that S. aureus (8 isolates), Escherichia coli (4 isolates), Enterobacter spp (2 isolates), Bacillus (1 isolates ), Streptococceus spp (1 isolates), and Pseudomonas spp (3 isolates), were the main isolates frequently associated with the mobile phones. Showed Percentage of bacterial isolates from the samples collected from mobile phones after calculating the total percentage of each isolate, found S. aureus, E. coli, Enterobacter spp, Bacillus, Streptococceus spp and Pseudomonas spp in the percentage of 42.10 %, 21.05 %, 10.52%, 5.26 %, 5.26 % and 15.78 % respectively. The results showed that mobile phones were contaminated with different types of bacteria mentioned above. Gram positive cocci, Streptococcus and Staphylococcus spp. were identified based on morphological characteristics. Gram negative bacilli, E. coli, Enterobacter, Bacillus and Pseudomonas were identified based on morphological characteristics. Nineteen isolates from 20 observed mobile phones belonging to the students. The highest prevalence in male was (13 isolates) and were percentage of bacteria isolated 66.66%, while in female were (6 isolates) and percentage of bacteria isolated 33.33%. Also showed results Percentage of total bacteria isolated of female and male, were 31.57% and 68.42 % respectively.
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Fatima Kareem Shandookh, Melad Khalaf Mohammed, and Ahmed Darweesh Jabbar. "Isolation, screening and characterization of hydrocarbon-degrading bacteria isolated from oil contaminated soil in Wasit province / Iraq." Journal of Wasit for Science and Medicine 16, no. 3 (September 14, 2023): 71–83. http://dx.doi.org/10.31185/jwsm.480.

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Eleven soil samples were collected from one of the oil sites in Wasit Governorate.65 bacterial isolates were isolated from soil samples by using BHM with 1% crude oil as carbon source. In primary and secondary screening (10) isolates of bacteria can remediate crude oil. Among the ten isolates, bacterial isolate named HD2 was most effective in bioremediation process. The results of biomass, optical density, and percentage of hydrocarbon remediation of HD2 were (0.63, 1.080 and 67 %) respectively. The results of morphological and biochemical tests showed that the bacterial isolate named HD2 belongs to the species Pseudomonas putida.
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Dissertations / Theses on the topic "Bacterial isolates"

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Reyes, Nikolle Susanne. "Marine bacterial isolates utilize unique mercury resistance mechanisms." Thesis, Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/25416.

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Puttanlek, Chureerat. "Microbial degradation of dichlobenil." Thesis, University of Kent, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314268.

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Saijonma-Koulumies, Leena E. M. "Bacterial interference in the control of canine pyoderma." Thesis, Royal Veterinary College (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368117.

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Luckarift, Heather Rosemary. "The production of chiral hydroxylated products from new bacterial isolates." Thesis, University of Warwick, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322689.

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Boyiri, Blaise B. "Probiotic Potential of Bacterial Isolates From ‘Amabere amaruranu’ Cultured Milk." Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etd/2389.

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Probiotics are viable nonpathogenic microbes that positively affect host health. Probiotics inhibit infection, activate immunity, and promote mucosal-barrier development. Many microbes have probiotic activity. Nonetheless, the selection of stable strains and their specific mechanism(s) of action are not fully elucidated. Bacteria from ‘Amabere amaruranu’ cultured milk from Kenya were isolated and identified by PCR sequence analysis of the 16S rRNA gene. Isolates were examined for stability to acid and bile, antimicrobial activity, mucin production, and degradation and sensitivity to antibiotics, hence their potential for probiotics. Lactobacillus isolates were acid unstable, bile-stable, nonmucinolytic, and presented antibacterial activity. L. rhamnosus cell fractions increased MUC4 and MUC3 expression in colon cells. Bacillus isolates were acid and bile stable, nonmucinolytic and lacked antimicrobial activity. In conclusion, Lactobacillus isolates that were nonmucinolytic, stable in bile, demonstrated antibacterial activity, sensitive to antibiotics, and stimulated increase MUC4 and MUC3 levels in colon cells could be potential probiotics.
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Lawson, Andrew Jeffrey. "The prevalence of Campylobacter species in human gastroenteritis : a molecular approach." Thesis, Open University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342935.

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Álvarez-Carretero, Sandra. "BACTpipe : Characterization of bacterial isolates based on whole-genome sequence data." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-15033.

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The technological advances have led to faster and more cost-effective sequencing platforms, making it quicker and more affordable to generate genomic sequence data. For the study of bacterial genome, two main methods can be used, whole-genome sequencing and metagenomic shotgun sequencing, of which the first is the mostly used in the past years. As a consequence of these advances, a vast amount of data is currently available and the need of bioinformatics tools to efficiently analyse and interpret it has dramatically increased. At present, there is a great quantity of tools to use in each step of bacterial genome characterization: (1) pre-processing, (2) de novo assembly, (3) annotation, and (4) taxonomic and functional comparisons. Therefore, it is difficult to decide which tools are better to use and the analysis is slowed down when changing from one tool to another. In order to tackle this, the pipeline BACTpipe was developed. This pipeline concatenates both bioinformatics tools selected based on a previous testing and additional scripts to perform the whole bacterial analysis at once. The most relevant output generated by BACTpipe are the annotated de novo assembled genomes, the newick file containing the phylogenetic relationships between species, and the gene presence-absence matrix, which the users can then filter according to their interests. After testing BACTpipe with a set of bacterial whole-genome sequence data, 60 genes out of the 18195 found in all the Lactobacillus species analysed were classified as core genes, i.e. genes shared among all these species. Housekeeping genes or genes involved in the replication, transcription, or translation processes were identified
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Löfmark, Sonja. "Incidence, emergence, persistence and mechanisms of antimicrobial resistance in clinical isolates and normal microbiota /." Stockholm, 2007. http://diss.kib.ki.se/2007/978-91-7357-127-2/.

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Banerjee, Sharmistha. "Partial purification, characterization and antibiosis of Bacteriocins produced by some lactic acid Bacterial isolates." Thesis, University of North Bengal, 1996. http://hdl.handle.net/123456789/989.

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Pereira, Rui Alexandre Martins. "Nitrile hydratase from a thermophilic Bacillius isolate." Thesis, University College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267488.

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Books on the topic "Bacterial isolates"

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Ward, T. Interactions of selected bacterial isolates with DBT and solubilized coal. S.l: s.n, 1990.

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Luckarift, Heather Rosemary. The production of chiral hydroxylated products from new bacterial isolates. [s.l.]: typescript, 1998.

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Gravelle, Louise N. The antibiotic resistance of bacteria isolated from dental unit waterlines. Sudbury, Ont: Laurentian University, School of Graduate Studies, 2005.

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Deppe, Uta. Degradation of crude oil at low temperatures by a newly isolated psychrotolerant bacterial consortium. Berlin: Mensch & Buch Verlag, 2003.

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Watkins, Peter Gareth. The corrosion of mild steel in the presence of two isolates of marine sulphate reducing bacteria. Portsmouth: University of Portsmouth, School of Pharmaceutical [sic.] and Biomedical Sciences, 1998.

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Davis, Robert Patrick Matthews. Effect of nutrient variation on the growth of a bacterial species isolated from an activated sludge plant. [S.l: The Author], 1985.

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Nazarali, Zaida. The effect of acidophilic bacteria on the leaching of low-grade Cu-Ni ore by different isolates of Acidithiobacillus ferrooxidans. Sudbury, Ont: Laurentian University, School of Graduate Studies, 2004.

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Watthanachōt, Čhančharat, ed. Kānsưksā prōtīn thī mī rit tān čhulinsī čhāk bǣkthīrīa thī yǣk dai čhāk fō̜ngnām thalē =: A study of antimicrobial proteins of marine bacterial isolate from some sponges : rāingān kānwičhai chabap sombūn. [Chonburi]: Sathāban Witthayāsāt thāng Thalē, Mahāwitthayālai Būraphā, 2006.

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Dawson, Susan. Other bacterial diseasesStaphylococcal zoonoses. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0026.

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Staphylococcal species are common commensals of the skin and mucous membranes of humans and animals but only in very recent years has zoonotic infections been recognised. They can also be associated with infection and disease, especially coagulase positive organisms. Staphylococcus aureus is relatively frequently carried by humans in the nasal passages and is a cause of infections in people including bacteraemias in hospitalised patients. More recently some strains of Staphylococcus aureus have acquired a resistance gene (mecA) which renders them resistant to meticillin (meticillin-resistant Staphylococcus aureus, MRSA). MRSA isolates are of major importance in healthcare situations as well as increasingly in the community. Animals can also be carriers of Staphylococcus aureus although less frequently than humans and MRSA can be carried or infect several different host species. For companion animals such as dogs and cats, the most frequently isolated MRSA strains are similar to the common local human healthcare strains; thus for the UK, EMRSA-15 and -16. This suggests a reverse zoonosis with spill over from the human population into their companion animals. In horses the situation is different, with some horses carrying or infected with human epidemic strains but others infected with strains less frequently seen in people. For food-producing animals the picture is different again with a particular strain, ST398, which appears to circulate endemically in animal populations, such as pigs, and can spill over into the human population where it can cause carriage as well as infection and disease. The transmission appears to be by direct contact with animals rather than through the food-chain.Where risk factors for infection with MRSA have been studied in animals they appear similar to some of the risks for human infection. Therefore, for control of MRSA in animals measures such as improved hygiene and good antibacterial stewardship are important.
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Peekate, Lekiah. Deciphering the Identity of Bacterial Isolates Through Biochemical and Physicochemical Tests: A Practical Guide. Independently Published, 2019.

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Book chapters on the topic "Bacterial isolates"

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Konwar, Bolin Kumar. "Molecular Identification of Bacterial Isolates." In Bacterial Biopolymers, 45–52. New York: Apple Academic Press, 2023. http://dx.doi.org/10.1201/9781003331636-7.

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Lounatmaa, Kari, Markus Haapasalo, Eero Kerosuo, and Hannele Jousimies-Somer. "S-Layers Found on Clinical Isolates." In Advances in Bacterial Paracrystalline Surface Layers, 33–43. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4757-9032-0_4.

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Dzink-Fox, JoAnn, and Margret Oethinger. "Identification of Mar Mutants among Clinical Bacterial Isolates." In Frontiers in Antimicrobial Resistance, 224–34. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817572.ch16.

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Höfle, Manfred G. "RNA Chemotaxonomy of Bacterial Isolates and Natural Microbial Communities." In Aquatic Microbial Ecology, 129–59. New York, NY: Springer New York, 1990. http://dx.doi.org/10.1007/978-1-4612-3382-4_6.

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Borkar, S. G. "Rapid Assessment of Plant Pathogenic Nature of Bacterial Isolates." In Laboratory Techniques in Plant Bacteriology, 43–44. Boca Raton : Taylor & Francis, 2017.: CRC Press, 2017. http://dx.doi.org/10.1201/9781315206882-8.

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Kumari, Rajni, K. Harshan, Prashanth Rajan, Anand Prem Rajan, and Thomas Theodore. "Screening of Bacterial Isolates from Coal Mining Region in Chhattisgarh." In Sustainable and Cleaner Technologies for Environmental Remediation, 99–112. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-29597-3_9.

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Moll, Henry, Laura Lütke, and Andrea Cherkouk. "Bacterial Diversity in Clay and Actinide Interactions with Bacterial Isolates in Relation to Nuclear Waste Disposal." In Radionuclides in the Environment, 209–29. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-22171-7_12.

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Zaghloul, Taha I., M. Al-Bahra, and H. Al-Azmeh. "Isolation, Identification, and Keratinolytic Activity of Several Feather-Degrading Bacterial Isolates." In Biotechnology for Fuels and Chemicals, 207–13. Totowa, NJ: Humana Press, 1998. http://dx.doi.org/10.1007/978-1-4612-1814-2_20.

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Tahmatsidou, V. I., and A. C. Cassells. "Elimination of Several Bacterial Isolates from Meristem Tips of Hydrangea spp." In Pathogen and Microbial Contamination Management in Micropropagation, 175–81. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-015-8951-2_21.

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Mujawar, Shama, Amr Adel Ahmed Abd El-Aal, and Chandrajit Lahiri. "Variant Analysis from Bacterial Isolates Affirms DnaK Crucial for Multidrug Resistance." In Bioinformatics and Biomedical Engineering, 237–48. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-45385-5_22.

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Conference papers on the topic "Bacterial isolates"

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Kumalasari, Yeni Indra, Agung Dian Kharisma, and Sri Yuwantiningsih. "Potential of Karimunjawa Island’s Plants as Antibiotic-Producing Endophytic Bacteria Sources." In The 2nd International Conference on Technology for Sustainable Development. Switzerland: Trans Tech Publications Ltd, 2022. http://dx.doi.org/10.4028/p-kv25ou.

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Endophytic bacteria have a great potential to be applied as biofertilizers and biopesticides, but their information as a source of antibiotics still needs to be developed and explored. The aim of this study was to investigate the potential sources of antibiotics in endophytic bacteria isolated from the stems of Setigi, Wahong, Bongko, Kalimosodo, Dewandaru, and Legundi plants on Karimunjawa Island. Molecular approaches were performed to isolate, characterize, and identify bacterial endophytes as potential antibiotic sources by plate assay and 16S rRNA gene sequence analysis. Dewandaru isolate was identified as gram-negative bacteria, whereas; gram-positive bacteria were detected in other isolates. Moreover, Setigi and Dewandaru isolates showed the highest level to inhibit the growth of Fusarium sp and displayed 99% similarity with antibiotic-producing bacteria, namely Bacillus pumilus and Bacillus cereus, respectively. These results indicate the possibility of antibiotic activities by Setigi and Dewandaru isolated. Therefore, it is assumed that both Setigi and Dewandaru isolates potentially appeared as new antibiotics sources from local plants. This study provides novel insight into the future production of novel antibiotics derived from plant-associated endophytic bacterial as a strategy for increasing the application of natural compounds to control plant diseases in agriculture.
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Rammadan ABDUL, Fatima, Maysoon Kh.ABBAS, Batool Abd Al Ameer BAQER, and Ihsan Ali RAHEEM. "ASSESSMENT OF THE LEVEL OF INTERLEUKIN- 10 AND INTERFERON -GAMMA IN CHILDREN INFECTED WITH AEROMONAS HEMOPHILIA ISOLATED FROM DIARRHEA." In VIII.International ScientificCongressofPure,AppliedandTechnological Sciences. Rimar Academy, 2023. http://dx.doi.org/10.47832/minarcongress8-4.

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Background: Aeromonas hydrophila causes bacterial diseases that lead to intestinal and extra-intestinal infections. Objective: The study aimed to isolate Aeromonas hydrophila from patients suffering diarrhea and evaluate the level of some interleukins in patients and compare them with control and resistance of bacteria to antibiotics. Materials and methods collect 150 stool samples of patients with diarrhea. Six isolates of A. hemophilia were isolated from children suffering diarrhea from some Baghdad hospitals. Results, all bacterial isolates were identified by the biochemical, cultural and microbial characteristics and confirmed by VITK2 System. The study showed the resistance of A. hemophilia to many antibiotics, the highest resistance to Tetracycline and Oxacillin and the rate of resistance was 100%, while the bacteria had the lowest resistance 16.7%, to to Erythromycin. The present results exhibited Interleukin-10 level was elevated in the patients and it was controlled by a significant level. The present results besides exhibited that the concentration of Interferon- gamma (IFN-γ) of patients and control group with nonsignificant difference
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M.Y. AL-TAEE, Sura, Amina G.O. AL-ANI, and Alaa Younis MAHDY. "16s rRNA SEQUENCING FOR IDENTIFICATION BACTERIA FROM FACE MASK OF STUDENTS IN MOSUL UNIVERSITY." In V. International Scientific Congress of Pure, Applied and Technological Sciences. Rimar Academy, 2022. http://dx.doi.org/10.47832/minarcongress5-6.

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The use of masks has increased recently under the conditions of the Covid-19 pandemic as a prevention from the Covid-19, the research dealt with the study of bacterial contamination of masks from the surrounding environment, where in addition to the ability of masks to prevent the spread of viruses and bacteria, they are likely to be a source of bacterial contamination from the face, hands and air. 45 samples of face masks were collected from students of Mosul University in Iraq and the face mask was printed directly on Nutrient agar for 5 sec. Sixty isolates were obtained purely ,Gram-positive bacteria were predominated after smearing the pure isolates and staining them with Gram stain. Gram-positive isolates were grown on mannitol salt agar, which showed that they were not capable of fermenting mannitol sugar, oxidase and catalase assay was also performed, which gave a positive result for catalase and negative for oxidase .Three isolates were selected for molecular diagnosis by 16S rRNA. According to the 16S rRNA gene sequencing, three isolates were designated as:- Staphylococcus equorumand two isolates for Staphylococcus saprophyticus.The isolates showed sensitivity to Amikacin,Vancomycin,Chloramphenicol and Erythromycin antibiotics except for the antibiotic ofloxacin, Staphylococcus equorum and one isolate for Staphylococcus saprophyticus were intermediate to it
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Sultana, Sharmin, Md Sad Salabi Sawrav, Snygdha Rani Das, Mehfuz Alam, Md Abdul Aziz, Md Al-Amin Hossain, and Md Azizul Haque. "Isolation and Biochemical Characterization of Cellulase Producing Goat Rumen Bacteria." In International Conference on Emerging Trends in Engineering and Advanced Science. AIJR Publisher, 2022. http://dx.doi.org/10.21467/proceedings.123.12.

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Cellulose is the most prevalent polymer on the planet and has long been utilized for a variety of industrial applications. The study's goal was to screen and isolate cellulase-producing bacteria from the rumen of a goat collected from different location of Dinajpur district. To do so, rumen content samples from two distinct goats were collected. In this investigation, rumen cellulase-producing bacteria were isolated and characterized after serial dilution of five isolates up to six fold and inoculation into Nutrient agar. Following that, all of the isolates were underwent Methyl Red (MR) test & Voges-Proskauer (VP) test to identify organism’s metabolic pathway, Triple Sugar Iron Agar (TSI) Test to determine bacterial ability to utilize sugar, Motility Indole and Urease activity test (MIU) to determine motility, Urease utilization and can produce Indole or not, Citrate utilization test to utilize citrate as carbon and energy source, Oxidase test, Catalase test to check the presence of catalytic enzyme. The result revealed the colonial characterization of bacteria and also where proven all five isolates are promising enough and superior in quality to produce cellulose.
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Siripornadulsil, Surasak, and Wilailak Siripornadulsil. "Characterization of Cadmium-Resistant Bacteria and Their Application for Cadmium Bioremediation." In ASME 2009 12th International Conference on Environmental Remediation and Radioactive Waste Management. ASMEDC, 2009. http://dx.doi.org/10.1115/icem2009-16072.

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On a global basis, trace-metal pollution is one of the most pervasive environmental problems. It is particularly difficult to prevent or clean up because the metals are toxic in their elemental form and cannot be decomposed. Bioremediation has been shown to be a powerful system for heavy metal pollution clean up and prevention. In this work, we characterized the cadmium (Cd)-resistant bacteria isolated from rice field soil downstream from zinc (Zn) mineralized area which the owners were contaminated at high level of cadmium content in their blood (>10 μgCd/g creatinine). We found that all 24 isolated bacteria tolerated toxic Cd concentrations (2,500 μM). In order to determine whether the Cd toxicity affected the growth of isolated bacteria, we grew the isolated bacterial cells in the absence and presence of toxic concentrations of CdCl2 (500 μM). In the absence of Cd, all isolated bacterial cells grew slightly better than in the presence of toxic concentrations of Cd. In addition, the Cd binding capacity of all isolated bacteria were very high, ranging from 6.38 to 9.38 log[Cd(atom)]/cell when grown in the presence of 500 μM CdCl2. Furthermore, the stability of Cd-bacteria complex of all isolated bacteria was affected by 1mM EDTA. When grown in the presence of 500 μM CdCl2, Cd-resistant isolates S2500-6, -8, -9, -15, -17, -18, -19, and -22 increasingly produced proteins containing cysteine (SH-group) (from 1.3 to 2.2 times) as well as 11 isolates of Cd-resistant bacteria, including S2500-1, -2, -3, -5, -6, -8, -9, -11, -16, -20, and -21, increasingly produced inorganic sulfide (1.5 to 4.7 times). Furthermore, the Sulfur K-edge X-ray absorption near-edge structure (XANES) spectroscopy studies indicated that Cd-resistant isolated S2500-3 precipitated amounts of cadmium sulfide (CdS), when grown in the presence of 500 μM CdCl2. The results suggested that these Cd-resistant bacteria have potential ability to precipitate a toxic soluble CdCl2 as nontoxic insoluble CdS. Interestingly, Cd-resistant bacteria isolated S2500-3, -8, -9,and -20 increased cadmium tolerance of Thai jasmine rice (Kao Hom Mali 105) when grown in the presence of 200 μM CdCl2. These 4 isolates also decreased cadmium concentration accumulation in Kao Hom Mali 105 plant at 61, 9, 6, and 17%, respectively when grown in the presence of 200 μM CdCl2. They were identified by 16S rDNA sequence analysis and classified as Cupriavidus taiwanensis (isolate S2500-3) and Pseudomonas aeruginosa (isolates S2500-8, -9, and -20).
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Hewagama, H. L., G. M. T. K. Somarathna, L. Herath, and S. E. Peiris. "Living Colours: Development of Microbial Culture Collection for Use as Microbial Colour Pigments in Textile Dyes." In SLIIT International Conference on Advancements in Sciences and Humanities 2023. Faculty of Humanities and Sciences, SLIIT, 2023. http://dx.doi.org/10.54389/ccoj7801.

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The textile industry is one of the largest worldwide polluters of clean water due to the heavy use of synthetic dyes. Synthetic dyes are harmful to aquatic life and to human health. To overcome this, natural dyes are being explored as a healthier and more eco-friendly alternative. Several advantages such as ease of extraction, availability, high yields and no seasonal variation make microbial pigments the most ideal source of natural pigments. This study was done to isolate colour pigment producing bacteria and fungi from soil collected from organic farms from various locations in Sri Lanka. In total, 9 pigment producing bacteria and 3 pigment producing fungi were isolated. Gause’s synthetic agar yielded the most pigmented isolates. Extracellular pigments produced by 5 of the bacterial isolates were extracted by a water based method. The antibacterial activity of the pigments in their crude and concentrated forms was tested using the well diffusion method against E.coli ATCC 8739 and Staphylococcus aureus ATCC 6538P. Inhibition zone against S.aureus was observed for both crude (12.33±0.58mm) and concentrated pigments (9.67±0.58mm) extracted from purple pigment producing bacterial isolate (BPU). This pigment has the potential to be used in antibacterial textile preparation. Extracted pigments were used to dye scoured cotton fabric with the use of 3% alum as mordant. Pigment from BPU isolate resulted in better coloured fabric.
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Samoilova, Anna. "Effect of phages isolated from different sources against fire blight pathogen." In 5th International Scientific Conference on Microbial Biotechnology. Institute of Microbiology and Biotechnology, Republic of Moldova, 2022. http://dx.doi.org/10.52757/imb22.29.

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Fire blight of rosaceous plants is one of the economically most important diseases of fruit trees caused by the bacterium Erwinia amylovora. Plants are extremely vulnerable for fire blight infection at the bloom stage. Blossom blight can lead to the great crop losses and even the plant death. Since chemical treatments are forbidden in time of blossoming, bacteriophages, highly specific bacterial viruses could be used for the disease control. Being the natural components of ecosystems, phages infect only bacteria sensitive to them, are non-toxic to plants, animals and humans and are adapted to the bacteria environment. It has been shown that bacterium E. amylovora expresses its major pathogenicity factors during immature pear tissues infection. Therefore, in this study, the ability of four virulent E. amylovora bacteriophages, isolated from the aerial parts of the affected plants (phage isolate 1 from quince tissues; phage isolate 2 from hawthorn, Republic of Moldova) and from natural water reservoirs near fruits orchards or wild rosaceous trees (phage isolates 3 and 4, Swiss Confederation) to inhibit E. amylovora growth in the immature pear tissues was evaluated. Immature pear slices were inoculated with suspensions of E. amylovora CFBP1430 and EaM contained 104 CFU/ml. After four hours incubation in the humidified chamber at 280C infected immature pear slices were treated with 107 PFU/ml of phage isolates. Pear slices, treated with sterile distilled water were used as a control. Symptoms were recorded at 1, 2, 3, 5, 6, 7 and 8 days after inoculation. For each bacteria strain/phage isolate combination tested pear slices were assayed in triplicate and each experiment was repeated at least two times. Immature pear slices infected with bacteria EaM displayed the first symptoms of the fire blight, ooze formation and light necrosis, one day after inoculation. Pear slices, infected with E. amylovora CFBP1430 demonstrated ooze and necrosis two days after inoculation. In the bacteria/phage combinations the first symptoms of the fire blight appeared on the sixth day after inoculation in the variants of EaM/phage isolate 3 and CFBP1430/phage isolate 3. On the seventh and eighth days after inoculation symptoms of the fire blight infection have been recorded in the EaM/phage isolate 2 and CFBP1430/ phage isolate 2, respectively. Bacteria/phage combinations EaM/phage isolate 4 and CFBP1430/ phage isolate 4 showed disease symptoms on the seventh day after inoculation. Immature pear slices in the variants EaM/phage isolate 1 and CFBP1430/phage isolate 1 showed necrotic lesion eight days after inoculation. Thus, phage isolate 4, detected in water was able to suppress growth of phytopathogenic E. amylovora just a day less than highly virulent phage isolate 1 detected in the quince tissues. The conducted experiments have demonstrated that bacteriophages isolated from water revealed high efficacy against bacteria E. amylovora and all studied phage isolates successfully inhibited the fire blight causative agent growth in the plant host tissues for about seven days. Hence it has been shown that treatment with bacteriophages for the fire blight control in the fruit orchard should be carried out weekly if environmental conditions are favorable for the disease development.
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Khalid MOHAMMED, Ansam, Nazih Wayes ZAID, and Mariam Hamdi ABDULKAREEM. "SECTION OF VETERINARY MEDICINE: MICROBIOLOGY, IMMUNITY AND VIROLOGY. THE BACTERIAL CONTAMINATION WITH PROTEUS AND E. COLI IN CERVIX AND UTERINE OF COWS DURING THE DIFFERENT ESTRUS PHASES." In VIII.International ScientificCongressofPure,AppliedandTechnological Sciences. Rimar Academy, 2023. http://dx.doi.org/10.47832/minarcongress8-15.

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The herein research was carried out in order to identified the presence of bacteria in cervix and uterine lumen in Iraqi cattle during the different estrus phase with focusing on Protus and E coli. Estrus phases were determined by the structures which found on ovary (follicular growth for pro-estrus, mature growing follicle for estrus, hemorrhagic corpus luteam for meta-estrus and active corpus luteam for di-eatrus). Forty cervical swabs (ten for each estrus phase) and forty uterine swabs (ten for each estrus phase) were taken from macroscopically healthy reproductive animals after slaughtering and cultivated on nutrient agar and blood agar, the bacterial isolation were identified with biochemical teats. The present study found that (65%) of cervical swabs were bacterial positive and the bacterial isolates were higher in the pro-estrus and meta-estrus phases 70% than estrus and diestrus 60%, the Protus spp. Could not been isolated from cervix or uterine during estrus phases, while E coli isolated during three first phases and disappear during diestrus phase, and appear as 10 single and 10 mixed isolated during follicular phase and metaestrus phase in cervical swabs. A total of five different microorganisms were isolated from cervical swabs (Escherichia coli, Streptococcus faecalis, Staphylococcus aureus, Staphylococcus hominies and Staphylococcus epidermidis) with twelve single isolation and fourteen mixed isolation. The present study found that (47.5%) of uterine swabs were bacterial positive and the bacterial isolates were higher in the pro-estrus, estrus and meta-estrus phases 50% than estrus and diestrus 40%, E coli isolated during estrus and diestrus phases only, and appear as 7 single and 2 mixed isolated during those two phases in uterine swabs. A total of five different microorganisms were isolated from uterine swabs (Escherichia coli, Streptococcus faecalis, Staphylococcus aureus, Staphylococcus hominies and Staphylococcus epidermidis) with fourteen single isolation and five mixed isolation.
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Sultana, Sharmin, Md Sad Salabi Sawrav, Md Bokhtiar Rahma, Md Shohorab Hossain, and Md Azizul Haque. "Isolation and Biochemical Characterization of Xylanase Enzyme Producing Bacteria from Goat Rumen." In International Conference on Emerging Trends in Engineering and Advanced Science. AIJR Publisher, 2022. http://dx.doi.org/10.21467/proceedings.123.1.

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The rumen microbial communities of ruminants are thought to be the most promising biochemical source of inordinately diversified and multi-functional cellulolytic enzymes with unique functional adaptations to improve biotechnological processes. The exploitation of rumen microbial genetic variety has been limited due to a lack of effective screening culture techniques and a lack of understanding of the rumen microbial genetic diversity. This study is conducted to isolate and characterize rumen bacteria from goat rumen that have capability to produce xylanase enzyme. Serial dilutions technique is applied to isolate bacteria from goat rumen and repeated tubing of the selectively enriched microbial cultures by using the specific media for rumen bacteria. Following that, all of the isolates were underwent Methyl Red (MR) test & Voges-Proskauer (VP) test to identify organisms metabolic pathway, Triple Sugar Iron Agar (TSI) Test to determine bacterial ability to utilize sugar, Motility Indole and Urease activity test (MIU) to determine motility, Urease utilization and can produce Indole or not, Citrate utilization test to utilize citrate as carbon and energy source, Oxidase test, Catalase test to check the presence of catalytic enzyme where all isolates found promising which indicates that all five isolates are superior and capable to produce xylanase.
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Rammadan ABDUL, Fatima, Ihsan Ali RAHEEEM, Alaa Laebi ABDULLAH, and Batool Abd Al Ameer BAQER. "DETECTION OF SOME VIRULENCE FACTORS AND ANTIBIOTICS RESISTANCE OF KLEBSIELLA PNEUMONIAE." In DETERMINATION OF THE ACTUAL INTENSITY BY CORRECTION OF THE EMISSION SPECTRUM LINES OF HEAVY METALS CONTAINED IN CRUDE OIL USING LASER INDUCED PLASMA –TECHNIQUE. Rimar Academy, 2022. http://dx.doi.org/10.47832/minarcongress4-9.

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Background: Infections of Klebsiella pneumoniae can include; diarrhea, septicemia, pneumonia, urinary tract infection and infections of soft tissues. Many factors are donated to K. pneumoniae pathogenicity particularly production of enzymes and formation of biofilm. Objective: find the relationship between the resistance of K. pneumoniae bacteria to antibiotics of quinolones and their ability to produce enzymes of beta lactamase. Materials and Methods: The Study included isolation and identification of (51) isolate of K. pneumoniae and (94) isolates of other bacteria from different clinical sources in some Baghdad hospitals. Results: The isolation and diagnosis of (51) isolates of K. pneumoniae from infection of urinary tract were 49.1%, infection of wounds were 31.3% and infection of burns were19.6%. All bacterial isolates were identified by the biochemical, cultural and microbial characteristics and confirmed by Api E20 System. I showed of β-lactamase test of Klebsiella pneumoniae revealed that (35) 68.6% isolates were positive. While 16 (31.4%) isolates were able to produce urease. Four groups of quinolones were tested by done the sensitivity test of isolates and results revealed the following percentage of resistant to Norfloxacin, Ofloxacin and ciprofloxacin consequently were (50.1%), (44.5%), (39.4%). whereas, the lower percent of resistant to Levofloxacin was (26.8%). In contrast, the βlactamase positive K. pneumoniae exhibited a high resistance in compare to isolates that negative for β-lactamase. The minimum inhibitory range concentrations of ciprofloxacin were arranged between (4-512 µg\ml). From isolates that resistant to Ciprofloxacin, the DNA plasmid was determined. Single plasmid bands were included in two isolates with same size and other isolates were confined free plasmid
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Reports on the topic "Bacterial isolates"

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Hannan, Trisha. Characterization of gram-positive bacterial isolates from burn victims. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.3053.

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Iliev, Mihail, Milena Mitova, Ralitsa Ilieva, Veneta Groudeva, and Petar Grozdanov. Bacterial Isolates from Rock Paintings of Magura Cave and Sensitivity to Different Biocides. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, May 2018. http://dx.doi.org/10.7546/crabs.2018.05.08.

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Thurston, Alison, Logan Gonzalez, Flora Laurent, Elizabeth Corriveau, and Robyn Barbato. Isolation and characterization of bacterial isolates from Alaskan permafrost for synthetic biology applications. Engineer Research and Development Center (U.S.), September 2023. http://dx.doi.org/10.21079/11681/47645.

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Operations in the Artic and other cold regions require technologies that can perform reliably under extreme cold conditions. Permafrost and frozen soils harbor a wide range of microorganisms that have adapted to extremely low temperatures and have unique metabolic capabilities relevant to military operations and that could be exploited to develop biotechnologies optimized for cold environments. Cold-tolerant bacteria (psychrophiles and psychrotrophs) are critical to the development of synthetic biology technologies meant to work in cold environments like the Arctic. Using bacteria isolated from Alaskan permafrost, we applied an experimental pipeline to test the best candidates for use as biological platforms, or chassis, for low-temperature synthetic biology. Since synthetic biology constructs will perform only as well as their chassis, it is critical that circuits expected to perform under extreme cold conditions are housed in chassis that are adapted to those conditions. We identified one permafrost isolate, PTI8, related to Rhodococcus fascians, that is capable of growing from −1°C to at least 25°C and which we experimentally confirmed to uptake and express the broad host range plasmid pBTK519, suggesting PTI8 is a candidate for use as a novel cold-adapted chassis for synthetic biology.
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Cytryn, Eddie, Mark R. Liles, and Omer Frenkel. Mining multidrug-resistant desert soil bacteria for biocontrol activity and biologically-active compounds. United States Department of Agriculture, January 2014. http://dx.doi.org/10.32747/2014.7598174.bard.

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Control of agro-associated pathogens is becoming increasingly difficult due to increased resistance and mounting restrictions on chemical pesticides and antibiotics. Likewise, in veterinary and human environments, there is increasing resistance of pathogens to currently available antibiotics requiring discovery of novel antibiotic compounds. These drawbacks necessitate discovery and application of microorganisms that can be used as biocontrol agents (BCAs) and the isolation of novel biologically-active compounds. This highly-synergistic one year project implemented an innovative pipeline aimed at detecting BCAs and associated biologically-active compounds, which included: (A) isolation of multidrug-resistant desert soil bacteria and root-associated bacteria from medicinal plants; (B) invitro screening of bacterial isolates against known plant, animal and human pathogens; (C) nextgeneration sequencing of isolates that displayed antagonistic activity against at least one of the model pathogens and (D) in-planta screening of promising BCAs in a model bean-Sclerotiumrolfsii system. The BCA genome data were examined for presence of: i) secondary metabolite encoding genes potentially linked to the anti-pathogenic activity of the isolates; and ii) rhizosphere competence-associated genes, associated with the capacity of microorganisms to successfully inhabit plant roots, and a prerequisite for the success of a soil amended BCA. Altogether, 56 phylogenetically-diverse isolates with bioactivity against bacterial, oomycete and fungal plant pathogens were identified. These strains were sent to Auburn University where bioassays against a panel of animal and human pathogens (including multi-drug resistant pathogenic strains such as A. baumannii 3806) were conducted. Nineteen isolates that showed substantial antagonistic activity against at least one of the screened pathogens were sequenced, assembled and subjected to bioinformatics analyses aimed at identifying secondary metabolite-encoding and rhizosphere competence-associated genes. The genome size of the bacteria ranged from 3.77 to 9.85 Mbp. All of the genomes were characterized by a plethora of secondary metabolite encoding genes including non-ribosomal peptide synthase, polyketidesynthases, lantipeptides, bacteriocins, terpenes and siderophores. While some of these genes were highly similar to documented genes, many were unique and therefore may encode for novel antagonistic compounds. Comparative genomic analysis of root-associated isolates with similar strains not isolated from root environments revealed genes encoding for several rhizospherecompetence- associated traits including urea utilization, chitin degradation, plant cell polymerdegradation, biofilm formation, mechanisms for iron, phosphorus and sulfur acquisition and antibiotic resistance. Our labs are currently writing a continuation of this feasibility study that proposes a unique pipeline for the detection of BCAs and biopesticides that can be used against phytopathogens. It will combine i) metabolomic screening of strains from our collection that contain unique secondary metabolite-encoding genes, in order to isolate novel antimicrobial compounds; ii) model plant-based experiments to assess the antagonistic capacities of selected BCAs toward selected phytopathogens; and iii) an innovative next-generation-sequencing based method to monitor the relative abundance and distribution of selected BCAs in field experiments in order to assess their persistence in natural agro-environments. We believe that this integrated approach will enable development of novel strains and compounds that can be used in large-scale operations.
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Nealson, Kenneth. Final Scientific Report: Bacterial Nanowires and Extracellular Electron Transfer to Heavy Metals and Radionuclides by Bacterial Isolates from DOE Field Research Centers. Office of Scientific and Technical Information (OSTI), December 2016. http://dx.doi.org/10.2172/1337164.

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Washburn, Quinn L., Savannah Spradlin, and Carolyn F. Weber. Addition of Zinc, Manganese, and Iron to Growth Media Triggers Antibiotic Production in Bacterial Isolates From the Lower Atmosphere. Journal of Young Investigators, March 2017. http://dx.doi.org/10.22186/jyi.32.2.7-11.

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Jorgensen, Frieda, John Rodgers, Daisy Duncan, Joanna Lawes, Charles Byrne, and Craig Swift. Levels and trends of antimicrobial resistance in Campylobacter spp. from chicken in the UK. Food Standards Agency, September 2022. http://dx.doi.org/10.46756/sci.fsa.dud728.

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Campylobacter spp. are the most common bacterial cause of foodborne illness in the UK, with chicken considered to be the most important vehicle of transmission for this organism. It is estimated there are 500,000 cases of campylobacteriosis in the UK annually, with Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) accounting for approximately 91% and 8 % of infections, respectively. Although severe infection in humans is uncommon, treatment is seldom needed for human infection but usually involves the administration of a macrolide (e.g., azithromycin) or a fluoroquinolone (e.g., ciprofloxacin). An increased rate of resistance in Campylobacter in chicken to such antimicrobials could limit effective treatment options for human infections and it is therefore important to monitor changes in rates of resistance over time. In this report we analysed trends in antimicrobial resistance (AMR) in C. jejuni and C. coli isolated from chicken in the UK. The chicken samples were from chicken reared for meat (ie. broiler chicken as opposed to layer chicken (ie. egg-laying chicken)) and included chicken sampled at slaughterhouses as well as from retail stores in the UK. Datasets included AMR results from retail surveys of Campylobacter spp. on chicken sampled in the UK from various projects in the time period from 2001 to 2020. In the retail surveys, samples were obtained from stores including major and minor retail stores throughout the UK (in proportion to the population size of each nation) and Campylobacter spp. testing was performed using standard methods with the majority of isolates obtained from direct culture on standard media (mCCDA). Data from national scale surveys of broiler chicken, sampling caecal contents and carcase neckskins at slaughterhouses, undertaken by APHA in 2007/2008, and between 2012 and 2018 were also included in the study. In the APHA-led surveys, Campylobacter were isolated using standard culture methods (culture onto mCCDA) and antimicrobial susceptibility testing was performed by a standard microbroth dilution method to determine the minimum inhibitory concentration (MIC) of isolates. Care was taken when comparing data from different studies as there had been changes to the threshold used to determine if an isolate was susceptible or resistant to an antimicrobial in a small number of scenarios. Harmonised thresholds (using epidemiological cut-off (ECOFF) values) were employed to assess AMR with appropriate adjustments made where required to allow meaningful comparisons of resistance prevalence over time. Data from additional isolates where resistance to antimicrobials were predicted from genome sequence data were also considered.
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Willis, C., F. Jorgensen, S. A. Cawthraw, H. Aird, S. Lai, M. Chattaway, I. Lock, E. Quill, and G. Raykova. A survey of Salmonella, Escherichia coli (E. coli) and antimicrobial resistance in frozen, part-cooked, breaded or battered poultry products on retail sale in the United Kingdom. Food Standards Agency, May 2022. http://dx.doi.org/10.46756/sci.fsa.xvu389.

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Frozen, breaded, ready-to-cook chicken products have been implicated in outbreaks of salmonellosis. Some of these outbreaks can be large. For example, one outbreak of Salmonella Enteritidis involved 193 people in nine countries between 2018 and 2020, of which 122 cases were in the UK. These ready-to-cook products have a browned, cooked external appearance, which may be perceived as ready-to-eat, leading to mishandling or undercooking by consumers. Continuing concerns about these products led FSA to initiate a short-term (four month), cross-sectional surveillance study undertaken in 2021 to determine the prevalence of Salmonella spp., Escherichia coli and antimicrobial resistance (AMR) in frozen, breaded or battered chicken products on retail sale in the UK. This study sought to obtain data on AMR levels in Salmonella and E. coli in these products, in line with a number of other FSA instigated studies of the incidence and nature of AMR in the UK food chain, for example, the systematic review (2016). Between the beginning of April and the end of July 2021, 310 samples of frozen, breaded or battered chicken products containing either raw or partly cooked chicken, were collected using representative sampling of retailers in England, Wales, Scotland and Northern Ireland based on market share data. Samples included domestically produced and imported chicken products and were tested for E. coli (including extended-spectrum beta-lactamase (ESBL)-producing, colistin-resistant and carbapenem-resistant E. coli) and Salmonella spp. One isolate of each bacterial type from each contaminated sample was randomly selected for additional AMR testing to determine the minimum inhibitory concentration (MIC) for a range of antimicrobials. More detailed analysis based on Whole Genome Sequencing (WGS) data was used to further characterise Salmonella spp. isolates and allow the identification of potential links with human isolates. Salmonella spp. were detected in 5 (1.6%) of the 310 samples and identified as Salmonella Infantis (in three samples) and S. Java (in two samples). One of the S. Infantis isolates fell into the same genetic cluster as S. Infantis isolates from three recent human cases of infection; the second fell into another cluster containing two recent cases of infection. Countries of origin recorded on the packaging of the five Salmonella contaminated samples were Hungary (n=1), Ireland (n=2) and the UK (n=2). One S. Infantis isolate was multi-drug resistant (i.e. resistant to three different classes of antimicrobials), while the other Salmonella isolates were each resistant to at least one of the classes of antimicrobials tested. E. coli was detected in 113 samples (36.4%), with counts ranging from <3 to >1100 MPN (Most Probable Number)/g. Almost half of the E. coli isolates (44.5%) were susceptible to all antimicrobials tested. Multi-drug resistance was detected in 20.0% of E. coli isolates. E. coli isolates demonstrating the ESBL (but not AmpC) phenotype were detected in 15 of the 310 samples (4.8%) and the AmpC phenotype alone was detected in two of the 310 samples (0.6%) of chicken samples. Polymerase Chain Reaction (PCR) testing showed that five of the 15 (33.3%) ESBL-producing E. coli carried blaCTX-M genes (CTX-M-1, CTX-M-55 or CTX-M-15), which confer resistance to third generation cephalosporin antimicrobials. One E. coli isolate demonstrated resistance to colistin and was found to possess the mcr-1 gene. The five Salmonella-positive samples recovered from this study, and 20 similar Salmonella-positive samples from a previous UKHSA (2020/2021) study (which had been stored frozen), were subjected to the cooking procedures described on the sample product packaging for fan assisted ovens. No Salmonella were detected in any of these 25 samples after cooking. The current survey provides evidence of the presence of Salmonella in frozen, breaded and battered chicken products in the UK food chain, although at a considerably lower incidence than reported in an earlier (2020/2021) study carried out by PHE/UKHSA as part of an outbreak investigation where Salmonella prevalence was found to be 8.8%. The current survey also provides data on the prevalence of specified AMR bacteria found in the tested chicken products on retail sale in the UK. It will contribute to monitoring trends in AMR prevalence over time within the UK, support comparisons with data from other countries, and provide a baseline against which to monitor the impact of future interventions. While AMR activity was observed in some of the E. coli and Salmonella spp. examined in this study, the risk of acquiring AMR bacteria from consumption of these processed chicken products is low if the products are cooked thoroughly and handled hygienically.
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9

Manulis-Sasson, Shulamit, Christine D. Smart, Isaac Barash, Laura Chalupowicz, Guido Sessa, and Thomas J. Burr. Clavibacter michiganensis subsp. michiganensis-tomato interactions: expression and function of virulence factors, plant defense responses and pathogen movement. United States Department of Agriculture, February 2015. http://dx.doi.org/10.32747/2015.7594405.bard.

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Clavibactermichiganensissubsp. michiganensis(Cmm), the causal agent of bacterial wilt and canker of tomato, is the most destructive bacterial disease of tomato causing substantial economic losses in Israel, the U.S.A. and worldwide. The goal of the project was to unravel the molecular strategies that allow Cmm, a Gram-positive bacterium, to develop a successful infection in tomato. The genome of Cmm contains numerous genes encoding for extracellular serine proteases and cell wall degrading enzymes. The first objective was to elucidate the role of secreted serine proteases in Cmm virulence. Mutants of nine genes encoding serine proteases of 3 different families were tested for their ability to induce wilting, when tomato stems were puncture-inoculated, as compared to blisters formation on leaves, when plants were spray-inoculated. All the mutants showed reduction in wilting and blister formation as compared to the wild type. The chpCmutant displayed the highest reduction, implicating its major role in symptom development. Five mutants of cell wall degrading enzymes and additional genes (i.e. perforin and sortase) caused wilting but were impaired in their ability to form blisters on leaves. These results suggest that Cmm differentially expressed virulence genes according to the site of penetration. Furthermore, we isolated and characterized two Cmmtranscriptional activators, Vatr1 and Vatr2 that regulate the expression of virulence factors, membrane and secreted proteins. The second objective was to determine the effect of bacterial virulence genes on movement of Cmm in tomato plants and identify the routes by which the pathogen contaminates seeds. Using a GFP-labeledCmm we could demonstrate that Cmm extensively colonizes the lumen of xylem vessels and preferentially attaches to spiral secondary wall thickening of the protoxylem and formed biofilm-like structures composed of large bacterial aggregates. Our findings suggest that virulence factors located on the chp/tomAPAI or the plasmids are required for effective movement of the pathogen in tomato and for the formation of cellular aggregates. We constructed a transposon plasmid that can be stably integrated into Cmm chromosome and express GFP, in order to follow movement to the seeds. Field strains from New York that were stably transformed with this construct, could not only access seeds systemically through the xylem, but also externally through tomato fruit lesions, which harbored high intra-and intercellular populations. Active movement and expansion of bacteria into the fruit mesocarp and nearby xylem vessels followed, once the fruit began to ripen. These results highlight the ability of Cmm to invade tomato fruit and seed through multiple entry routes. The third objective was to assess correlation between disease severity and expression levels of Cmm virulence genes and tomato defense genes. The effect of plant age on expression of tomato defense related proteins during Cmm infection was analyzed by qRT-PCR. Five genes out of eleven showed high induction at early stages of infection of plants with 19/20 leaves compared to young plants bearing 7/8 leaves. Previous results showed that Cmm virulence genes were expressed at early stages of infection in young plants compared to older plants. Results of this study suggest that Cmm virulence genes may suppress expression of tomato defense-related genes in young plants allowing effective disease development. The possibility that chpCis involved in suppression of tomato defense genes is currently under investigation by measuring the transcript level of several PR proteins, detected previously in our proteomics study. The fourth objective was to define genome location and stability of virulence genes in Cmm strains. New York isolates were compared to Israeli, Serbian, and NCPPB382 strains. The plasmid profiles of New York isolates were diverse and differed from both Israeli and Serbian strains. PCR analysis indicated that the presence of putative pathogenicity genes varied between isolates and highlighted the ephemeral nature of pathogenicity genes in field populations of Cmm. Results of this project significantly contributed to the understanding of Cmm virulence, its movement within tomato xylem or externally into the seeds, the role of serine proteases in disease development and initiated research on global regulation of Cmm virulence. These results form a basis for developing new strategies to combat wilt and canker disease of tomato.
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10

Michel Jr., Frederick C., Harry A. J. Hoitink, Yitzhak Hadar, and Dror Minz. Microbial Communities Active in Soil-Induced Systemic Plant Disease Resistance. United States Department of Agriculture, January 2005. http://dx.doi.org/10.32747/2005.7586476.bard.

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Induced Systemic Resistance (ISR) is a highly variable property that can be induced by compost amendment of potting media and soils. For example, previous studies showed that only 1 of 79 potting mixes prepared with different batches of mature composts produced from several different types of solid wastes were able to suppress the severity of bacterial leaf spot of radish caused by Xanthomonas campestris pv. armoraciae compared with disease on plants produced in a nonamended sphagnum peat mix. In this project, microbial consortia in the rhizosphere of plants grown in ISR-active compost-amended substrates were characterized. The plants used included primarily cucumber but also tomato and radish. Rhizosphere microbial consortia were characterized using multiple molecular tools including DGGE (Israel) and T -RFLP (Ohio) in both ISR-active field plots and potting media. Universal as well as population-specific bacterial and fungal PCR primers were utilized. T -RFLP analyses using universal bacterial primers showed few significant differences in overall bacterial community composition in ISR-active and inactive substrates (Ohio). In addition, the community members which were significantly different varied when different ISR-activecomposts were used (Ohio). To better characterize the shifts in microbial community structure during the development of ISR, population specific molecular tools were developed (Israel, Ohio).-PCR primers were designed to detect and quantify bacterial groups including Pyrenomycetes, Bacillus, Pan toea, Pseudomonas, Xanthomonas and Streptomyces as well as Trichoderma and Fusarium; two groups of fungi that harbor isolates which are ISR active (Isreal and Ohio). Bacterial consortia associated with cucumber plants grown in compost-amended potting mixtures were shown to be dominated by the phylogenetic taxon Bacteroidetes, including members of the genus Chryseobacterium, which in some cases have been shown to be involved in biocontrol (Israel). Nested-PCR-DGGE analyses coupled with long l6S rDNA sequencing, demonstrated that the Chryseobacteriumspp. detected on seed and the root in compost-amended treatments were derived from the compost itself. The most effective ISR inducing rhizobacterial strains were identified as Bacillus sp. based on partial sequencing of l6S rDNA. However, these strains were significantly less effective in reducing the severity of disease than Trichoderma hamatum382 (T382). A procedure was developed for inoculation of a compost-amended substrate with T -382 which consistently induced ISR in cucumber against Phytophthora blight caused by Phytophthora capsiciand in radish against bacterial spot (Ohio). Inoculation of compost-amended potting mixes with biocontrol agents such as T -382 and other microbes that induce systemic resistance in plants significantly increased the frequency of systemic disease control obtained with natural compost amendments.
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