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Mawardi, Mira, Agustin Indrawati, Angela Mariana Lusiastuti, and I. Wayan Teguh Wibawan. "CHARACTERIZATION OF SPORE-FORMING BACTERIA ISOLATED FROM TILAPIA (OREOCHROMIS NILOTICUS) AND THEIR POTENTIAL FOR A PROBIOTIC CANDIDATE." Indonesian Aquaculture Journal 18, no. 2 (December 19, 2023): 105. http://dx.doi.org/10.15578/iaj.18.2.2023.105-114.
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Gram-positive spore bacteria are widely used as probiotics in general sectors. However, there are still limited bacterial isolates as probiotic candidates available from indigenous isolates, especially in aquaculture. This study aimed to obtain potential spore-forming isolates as probiotic candidate for tilapia. Tilapia fish samples were collected from Sukabumi, Ciamis, Serang, and Papua. Bacterial isolates were isolated from the digestive tract of tilapia. Bacteria were identified based on their morphological, molecular characteristics, complete genome composition, and cell surface identification based on hydrophobic properties. In this study, six bacteria were isolated and identified by molecular characteristics using 16S rRNA sequences. Based on the phylogenetic analysis, the 9 PP isolate was Priestia megaterium basonym: Bacillus megaterium, CMS 16N isolate was Brevibacillus halotolerans, PPN 10 isolate was Bacillus sp., 3.1 SKBM isolate was Bacillus mycoides, CMS 22 N and SRG32 isolate were Bacillus subtilis. Six bacteria had different phenotypicals, ATGC sequence compositions, and a higher proportion of total G~C sequence composition above 50%. The coherent cell surface hydrophobicity test was positive on the SAT, SA, AA, and compact growth patterns in soft-agar media for 9 PP, CMS 22 N, and SRG32 isolates. From our study, the indigenous spore-forming bacteria isolated from tilapia stomachs are enzymatic bacteria, which have a strong attachment to host tissue and high potential as a probiotic candidate for fish. Various hydrophobicity test results from each isolate indicate that the protein composition in the cell surface is different.
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Amelia, Titik Fadilah, Ace Baehaki, and Herpandi Herpandi. "Aktivitas Reduksi Merkuri pada Bakteri yang Diisolasi dari Air dan Sedimen di Sungai Musi." Jurnal FishtecH 5, no. 1 (September 25, 2016): 94–106. http://dx.doi.org/10.36706/fishtech.v5i1.3522.
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This study aims to isolate mercury resistant bacteria from water and sediment in Pulau Salah Nama which located on the River Musi Palembang, characterized mercury resistant bacteria growing at the highest concentration of HgCl2 and test the power of these bacteria in reducing mercury. The research was conducted from June 2015 until September 2015 using experimental methods laboris and descriptive data analysis. Reasecrh consissted of several stages, including sampling, bacterial isolation, characterization to determine the type of bacteria and mercury reducing power of bacteria. Isolation of bacteria produced 10 isolates. A1 was bacteria isolated from water and A2 was bacteria isolated from sediment. From 10 isolates selected 2 isolates of the higest concentration of HgCl2. Selected isolate A1 have the lowest mercury reduction power of 39.26% and selected isolate A2 have the highest mercury reduction power of 65.93%. In control medium without inoculant a decline in mercury concentration of 39.44%. Based on the characterization of the bacteria biochemical activity known A1 and A2 were Bacillus subtilis
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Reshetnikov, M. V., and V. P. Patyka. "Bacteria-antagonists of the agents of soryz bacterial diseases." Agricultural Science and Practice 10, no. 3 (February 28, 2024): 46–60. http://dx.doi.org/10.15407/agrisp10.03.046.
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Aim. To isolate and identify bacteria with antagonist properties for biocontrol of the agents of bacterial diseases of soryz (Sorghum oryzoidum) and sorghum crops. Methods. The studies were conducted in 2021-2023. Spore-forming bacteria were isolated from the soryz samples, collected in the fields of the experimental farm of the Uman National Horticulture University (Cherkasy region, Uman). Lactic acid bacteria were isolated from soryz plants, collected in the private land plot, located between the villages of Teolyn, Vladyslavchyk, Kniazhyky in Monastyryshche com- munity, Uman district, where Pershotravneve hamlet used to be situated. A total of 1,250 samples were analyzed. The experiment had three repeats. Spore-forming and lactic acid bacteria were isolated from the surface of soryz plants on the firm ripe stage in summer while isolating phytopathogenic bacteria. The isolates of lactic acid bacteria- antagonists were also isolated from the inner part of winter stubble stalk of soryz, collected from the tilled field. The antagonistic activity of the strains of lactic acid bacteria and spore-forming bacteria, isolated from different ecological niches, to phytopathogens of soryz and sorghum crops was determined in vitro. The strains of Pseudomonas syringae, the agents of soryz bacterial spots, were used as test-cultures: 211141a, 211141, 210341, 21034, and 210521, along with the collection strains of phytopathogens: Pseudomonas syringae 8299, Pseudomonas syringae subsp. syringae UKM B-1021, X. oryzae 8375, Dickeya chrysanthemi 8683, Diskeya chryzanthemi 8683. The antagonistic activity of the extracted isolates of spore-forming and lactic acid bacteria was studied using the method of radial strokes (joint cultivation of the antagonist and the strains under investigation). The bacterial isolates were deemed inactive if the growth delay zone was 0–5 mm (–), from 5 to 10 mm (+) – low activity, 11–20 mm (++) – moderate activity, over 20 mm (+++) – high activity regarding the test-cultures. To check the effect of the isolate-antagonist of phytopathogenic bacteria, artificial infecting was conducted in the field conditions. For this purpose, a diurnal culture of the antagonist was introduced into the stalk of plants in the concentration of 1×108 colony-forming units, and 24 h later, a culture of test-strain of the phytopathogen was administered above the previous puncture. The results were evaluated 7–14 days after the artificial infection. The experiment had three repeats. The isolates of bacteria which demonstrated their an- tagonistic properties regarding the phytopathogenic bacteria were identified by their morphological properties, Gram staining, catalase test, profile of carbohydrate fermentation and mass-spectrometry (MALDI-TOF – Matrix Assisted Laser Desorption/Ionization) using VITEK MS mass-spectrometer. Results. Thirty-eight spore-forming bacterial iso- lates were extracted from soryz; among these, 21030, 21095, 21040, ASV1, ASV3, B4 demonstrated their antagonistic activity towards the investigated phytopathogenic bacteria. Isolate 21040 showed high antagonistic activity to most test-strains of P. syringae from soryz (the zone of negative culture – 23–30 mm) and lower activity regarding the collection cultures. Isolates B4 and AVS3 demonstrated their selective activity regarding the investigated phytopatho- gens. Twenty isolates of lactic acid bacteria were extracted. Higher antagonistic activity was noted for the isolates of lactic acid bacteria 8/1 and F1 to the strains of P. syringae, isolated from soryz and collection cultures. The highest antagonistic activity of isolate 8/1 was noted regarding test-strains of P. syringae 210521 and X. oryzae 8375 (the zone of negative culture – 40–35 mm). In the field conditions, the treatment of sorghum plants with F1 affected the pathological process that developed due to the impact of the phytopathogenic bacteria P. syringae, which led to the reduction in disease symptoms. The taxonomic position of the isolates of bacteria, which seem to be promising for the control of disease agents, was determined. In terms of morphology of cells and colonies, the biochemical profile, and mass-spectrometry MALDI-TOF, the spore-forming isolates 21040 and B4 were identified as Bacillus subtilis, and ASV3 – as Bacillus vallismortis. The identified isolates of lactic acid bacteria were Lactobacillus pentosus F1 and Lactobacillus sakei 8/1. Conclusions. In addition to phytopathogenic bacteria, from soryz plants we isolated the strains of spore-forming bacteria Bacillus subtilis 21040, B4, Bacillus vallismortis AVS3 and such lactic acid bacteria as Lactiplantibacillus pentosus and Lactobacillus sakei 8/1 (Latilactobacillus sakei 8/1), promising for the elaboration of methods for the biocontrol of the agents of bacterial diseases.
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Msango Soko, K. R., R. C. Bhattacharya, B. Ramakrishnan, K. Sharma, and S. Subramanian. "Functional characterization of bacteria isolated from different gut compartments of white grub, Anamola dimidiata, larvae." Journal of Environmental Biology 41, no. 6 (November 15, 2020): 1526–35. http://dx.doi.org/10.22438/jeb/41/6/mrn-1420.
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Aim: The aim of the present study was to isolate and characterize cellulolytic, lipolytic and nitrate reductase activities in the bacteria isolated from the gut of white grub, Anamola dimidiata larvae Methodology: Field collected third instar scarab larvae were dissected under aseptic conditions and inoculated on different bacteriological media to isolate gut bacteria. Identification of these isolates was carried out by amplifying and sequencing the 16S rRNA gene and comparing with their closest relatives in GenBank. Cellulolytic, lipolytic and nitrate reductase activities were assayed using Carbonmethyl cellulose (CMC), Rhodamine B and nitrate broth media. Results: The majority of culturable bacteria in the gut of A. dimidiata belonged to two phyla: Firmicutes (62.5%) and Proteobacteria (37.5%). Forty aerobic and eleven anaerobic bacterial strains were isolated and tested for cellulolytic, lipolytic and nitrate reductase activity, and twenty seven and thirty one cellulolytic and lipolytic gut bacteria were identified, respectively, with 19 isolates exhibiting both activities whereas ten facultative anaerobic bacteria isolates were positive for nitrate reductase activity. Interpretation: These bacterial isolates may be good sources for profiling novel isolates and enzymes for industrial use besides identifying new solutions for pest control.
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Astley, Roger A., Md Huzzatul Mursalin, Phillip S. Coburn, Erin T. Livingston, James W. Nightengale, Eddy Bagaruka, Jonathan J. Hunt, and Michelle C. Callegan. "Ocular Bacterial Infections: A Ten-Year Survey and Review of Causative Organisms Based on the Oklahoma Experience." Microorganisms 11, no. 7 (July 13, 2023): 1802. http://dx.doi.org/10.3390/microorganisms11071802.
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Ocular infections can be medical emergencies that result in permanent visual impairment or blindness and loss of quality of life. Bacteria are a major cause of ocular infections. Effective treatment of ocular infections requires knowledge of which bacteria are the likely cause of the infection. This survey of ocular bacterial isolates and review of ocular pathogens is based on a survey of a collection of isolates banked over a ten-year span at the Dean McGee Eye Institute in Oklahoma. These findings illustrate the diversity of bacteria isolated from the eye, ranging from common species to rare and unique species. At all sampled sites, staphylococci were the predominant bacteria isolated. Pseudomonads were the most common Gram-negative bacterial isolate, except in vitreous, where Serratia was the most common Gram-negative bacterial isolate. Here, we discuss the range of ocular infections that these species have been documented to cause and treatment options for these infections. Although a highly diverse spectrum of species has been isolated from the eye, the majority of infections are caused by Gram-positive species, and in most infections, empiric treatments are effective.
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Talip Abd-alla, Mays, Mohsen Hashim Risan, and Athraa H. Muhsin. "Microbial Contamination and Identification of Bacterial for Mobiles Phones in Iraq." Al-Kufa University Journal for Biology 7, no. 2 (August 30, 2015): 50–56. http://dx.doi.org/10.36320/ajb/v7.i2.8017.
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This study was conducted to isolate and identify bacteria contaminants on a mobile phone. The samples were collected randomly from 20 mobile phones. This study was conducted between October to December, 2016 at College of Biotechnology, Al-Nahrain University. The isolated colonies were then sub cultured in nutrient agar and slants in order to obtain pure culture of all the six colonies. Six genera of bacteria were identified from positive cultures. In all, 20 swab samples of mobile phone were randomly examined, 19 bacterial isolates were identified from mobile phones were found contaminated with microbiota. The highest prevalence of Staphylococcus aureus was observed in mobile phones. The research findings indicated that S. aureus (8 isolates), Escherichia coli (4 isolates), Enterobacter spp (2 isolates), Bacillus (1 isolates ), Streptococceus spp (1 isolates), and Pseudomonas spp (3 isolates), were the main isolates frequently associated with the mobile phones. Showed Percentage of bacterial isolates from the samples collected from mobile phones after calculating the total percentage of each isolate, found S. aureus, E. coli, Enterobacter spp, Bacillus, Streptococceus spp and Pseudomonas spp in the percentage of 42.10 %, 21.05 %, 10.52%, 5.26 %, 5.26 % and 15.78 % respectively. The results showed that mobile phones were contaminated with different types of bacteria mentioned above. Gram positive cocci, Streptococcus and Staphylococcus spp. were identified based on morphological characteristics. Gram negative bacilli, E. coli, Enterobacter, Bacillus and Pseudomonas were identified based on morphological characteristics. Nineteen isolates from 20 observed mobile phones belonging to the students. The highest prevalence in male was (13 isolates) and were percentage of bacteria isolated 66.66%, while in female were (6 isolates) and percentage of bacteria isolated 33.33%. Also showed results Percentage of total bacteria isolated of female and male, were 31.57% and 68.42 % respectively.
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Siangpro, Noppadon, Songkran Chuakrut, Wanna Sirimanapong, Somboon Tanasupawat, Wongsakorn Phongsopitanun, Bunyarit Meksiriporn, Jarungwit Boonnorat, Siripun Sarin, Siriwat Kucharoenphaibul, and Rumpa Jutakanoke. "Lactiplantibacillus argentoratensis and Candida tropicalis Isolated from the Gastrointestinal Tract of Fish Exhibited Inhibitory Effects against Pathogenic Bacteria of Nile Tilapia." Veterinary Sciences 10, no. 2 (February 7, 2023): 129. http://dx.doi.org/10.3390/vetsci10020129.
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Nile tilapia is one of the most consumed farmed fish in the world. The outbreak of pathogenic bacterial diseases causes high mortality rates and economic losses in Nile tilapia farming. Antibiotic administrations are commonly utilized to inhibit and prevent bacterial infections. However, antibiotics are expensive and cause serious concerns for antibiotic resistance in fish that can be potentially transferred to humans. As an alternative solution, probiotics can be used to prevent infection of pathogenic bacteria in fish. In this work, both bacteria and yeast were isolated from fish gastrointestinal tracts and their inhibitory activity against Nile tilapia pathogenic bacteria was evaluated, as well as other probiotic properties. In this study, 66 bacteria and 176 acid tolerant yeasts were isolated from fish gastrointestinal tracts. Of all isolated microorganisms, 39 bacterial and 15 yeast isolates with inhibitory effect against pathogens were then examined for their probiotic properties (acidic and bile salt resistance, adhesion potential, and biofilm formation), formation of antibacterial factor survival rate under simulated gastrointestinal fluid, and safety evaluation. AT8/5 bacterial isolate demonstrated probiotic properties and the highest inhibition against all 54 tested pathogens while YON3/2 yeast isolate outperformed the inhibitory effect among all yeast isolates. These two probiotic isolates were further identified by 16S rDNA and the D1/D2 domain of 26S rDNA sequence analysis for bacterial and yeast identification, respectively. AT8/5 and YON3/2 showed the highest similarity to Lactiplantibacillus argentoratensis and Candida tropicalis, respectively. This is the first report on isolated L. argentoratensis and C. tropicalis with antipathogenic bacteria of Nile tilapia properties. Collectively, AT8/5 and YON3/2 could be potentially used as promising alternatives to existing antibiotic methods to prevent pathogenic bacteria infection in Nile tilapia farming.
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Fajri, Muhammad Danial, Subehan Lallo, and Sartini Sartini. "PRELIMINARY STUDIES ON ISOLATION AND SCREENING OF ANTIBIOTIC PRODUCING SYMBIOTIC BACTERIA FROM STARFISH (Protoreaster nodosus) COLLECTED FROM COASTAL AREA TAKALAR REGENCY, INDONESIA." Journal of Experimental Biology and Agricultural Sciences 9, no. 2 (April 25, 2021): 183–88. http://dx.doi.org/10.18006/2021.9(2).183.188.
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Marine organisms are well known for the availability of bioactive compounds which have various biological activities including antibacterial activity. Likewise, their symbiotic bacteria can also produce compounds that have similar activities. The purpose of this study was to isolate and screen the symbiotic bacteria from starfish (Protoreaster nodosus) collected from coastal area Takalar Regency, South Sulawesi, Indonesia. Isolation was carried out by the pour plate method using nutrient agar medium dissolved in sterile seawater. The isolated symbiotic bacteria were purified by using the quadrant method. The pure isolate was culture through submerged fermentation using nutrient broth media enriched with 1% yeast extract and sterile seawater for 7 days. The selected symbiont bacterial isolates were tested for their antibacterial activity against gram-positive and gram-negative bacteria using disc diffusion assays. The results of fermentation were separated from the biomasses and tested for antibacterial activity against Staphylococcus aureus (S. aureus, ATCC 25923), Bacillus subtilis (B. subtilis, ATCC 6633), Salmonella typhi (S. typhi, NCTC 786), and Escherichia coli (E. coli, ATCC 25923). The results of study revealed that four symbiotic bacteria (SB 1T, SB 2T, SB 3T, and SB 4T) were successfully isolated. All the SB isolates have good antibacterial activity against all tested bacterial strains with an average diameter of inhibition zone larger than 11 mm. Among all isolates, isolate SB 4T showed a remarkable size of zones growth inhibition (> 15 mm) against all tested bacterial strains. Thus, the symbiotic bacteria isolated from P. nodosus in this study have a promising broad-spectrum antibacterial activity.
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Qays, Hawra, Asia Fadhile Almansoory, and Israa Abdulwahab Al-Baldawi. "Interaction Between Typha domingensis and Bacteria Bacillus sp. to Treatment of Wastewater Polluted by Kerosene." IOP Conference Series: Earth and Environmental Science 1215, no. 1 (July 1, 2023): 012047. http://dx.doi.org/10.1088/1755-1315/1215/1/012047.
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Abstract Water pollution with Kerosene is considered as a serious environmental problem in Basrah. Typha domingensis was used to treat kerosene-contaminated water in the construction wetland system. The Isolated bacteria was the most efficient in degradation Kerosene. Bacteria isolated from contaminated water by hydrocarbons of four stations in Basrah, these isolates were tested to show efficiency in degrading hydrocarbons to be used in phytoremediation of water contamination with hydrocarbons. Twelve bacterial isolates were identified as Bacillus sp. The Isolated bacteria (B11) was the most efficient in degradation oil hydrocarbons based on these used in the phytotoxicity test. The phytotoxicity experiment was conducted for 72 days in two glass basins containing water contaminated with 5% kerosene concentration with T. domingensis. The Isolate bacterial were added to two glass basins to test the interaction of the bacterial degradation with the plant and two contaminated ponds without plant and two with pollutant and bacteria without plant with control basin free from pollutant, the temperature between (20.6-36.2)°C, dissolved oxygen between (2.5-7.4) mg / L. The rate of kerosene removal from the water contaminated in the ponds of the plant without bacteria and plant with bacteria, bacteria only and control (76.5%), (81.9)%, (74.1%), (57.4) respectively.
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Sedijani, Prapti, Bq Novi Aprilia, Dewa Ayu Citra Rasmi, and Kusmiyati Kusmiyati. "Isolation and Screening of Amilolytic Bacteria Isolate from Cassava (Manihot utilissima)." Jurnal Biologi Tropis 23, no. 4 (September 5, 2023): 226–32. http://dx.doi.org/10.29303/jbt.v23i4.5604.
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Amylase is an enzyme capable of hydrolyzing starch into simpler sugars. The use of amylase enzymes is urgently needed in various industrial fields, therefore the search for sources of amylase enzyme producers continues to be carried out for sources of amylase enzyme producers. Each industrial field has specific requirements for amylase enzymes. This study aims to isolate bacteria capable of producing amylase enzyme and to determine the amylase activity of bacteria isolated from cassava (Manihot utilissima) when incubated at different temperatures (room temperature and 30℃) and pH levels (7, 8, 9, and 10). The research involved bacterial isolation, testing the amylase activity of the isolated amylolytic bacteria at varying pH and temperatures, and characterizing the amylolytic bacteria. The amylolytic bacterial activity was qualitatively measured based on the clear zone's area formed around the colony. From this study 5 isolates showing amylolytic activity were obtained. The amylolytic activity index varied among the isolates, ranging from 0.12 to 0.59. The highest amylolytic activity index (0.59) was performed by S4 isolate on pH 10 medium incubated at 30℃. Microscopic and gram staining analysis suggest that two isolates were gram-negative coccus bacteria and three of them were gram-positive coccobaccilus bacteria.
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Gusmawartati, Agustian, Herviyanti, and Jamsari. "Isolation of Cellulolytic Bacteria from Peat Soils as Decomposer of Oil Palm Empty Fruit Bunch." Journal of Tropical Soils 22, no. 1 (January 2, 2017): 47–53. http://dx.doi.org/10.5400/jts.2017.v22i1.47-53.
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The aim of the research was to find out potential strainsof cellulolytic bacteria isolated from two tropical peat soils and to studythe potency of the isolated bacteria to decompose oil palm empty fruit bunch (EFB). The research was carried out in two stages: (1) isolation of cellulolytic bacteria from peat soils and (2) testing the potency of isolated bacteria to decompose oil palm EFB. The cellulolytic bacteria were isolated from two peat soils, i.e. a natural peat soil (forest) and a cultivated peat soil (has been used as agriculture land). Isolation of cellulolytic bacteria was conducted by preparing a series dilution of culture solutions using a streak plate method in a carboxymethyl cellulose(CMC) selective medium.Isolates that were able to form clear zones surrounding their bacterial colony were further tested to study the potency of the isolates to decompose cellulose in oil palm EFB. The cellulolytic activity of the selected isolates were further determined via production of reducing sugars in an oil palm EFB liquid medium using Nelson-Somogyi method. The results showed that there are six isolates of cellulolytic bacteria that have been identified in two tropical peat soils used in the current study. Two isolates are identified in a natural peat soil (forest) and four isolates are identified in a cultivated peat soil. The isolates collected are identified as Bacillus sp., Pseudomonassp. and Staphylococcus sp. Among the isolates, an isolate of GS II-1 produces the highest concentration of reducing sugars, namely 0.1012 unitmL-1or 101 ppm, indicating that the isolate of GS II-1 is highly potential to decompose oil palm EFB. Therefore, the isolate of GS II-1 can be used as a decomposer in the bio-conversion processes of oil palm EFB.Keywords: isolation, bacteria, cellulolytic, oil palm empty fruit bunch (EFB), peat soil
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Golaviya, Akash V., Suchit S. Pandya, Pratyush G. Patankar, Harshrajsinh B. Joddha, Chirag M. Chavda, Bharat B. Bhanderi, and Mayurdhvaj K. Jhala. "Isolation, Identification and Antibiogram of Cloacal Flora from Apparently Healthy Caged Zoo Birds." Indian Journal of Veterinary Sciences & Biotechnology 19, no. 1 (January 10, 2023): 38–42. http://dx.doi.org/10.48165/ijvsbt.19.1.09.
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Antibiotics are routinely used in animals and birds to prevent and cure bacterial infections. Various multidrug resistance bacteria have been detected in caged zoo birds, however there is a little known about their resistance pattern. In current study we isolated and identified cloacal bacteria from 50 zoo birds and compared their antimicrobial susceptibility. A total of 28 cloacal samples (56%) were found positive for different bacteria by selective culture. The bacteria isolated from different types of caged birds were Escherichia coli (26%), Staphylococcus spp. (8%), Streptococcus spp. (4%) and some unidentified Gram-positive and Gram-negative bacteria (14%). Of these isolates, E. coli was the most frequent isolate. The results of antibiotic sensitivity tests revealed that cefotaxime and sulphadiazine were highly (>60%) resistant to isolated cloacal microflora of zoo birds. However, the antibiotics such as ceftriaxone and amikacin showed moderate to high sensitivity against almost all the bacterial isolates. Of these, co-trimoxazole was found to be consistently highly effective (100%) on all the E. coli isolates. Overall, the results showed that zoo birds can be carrier of multidrug resistant organisms including Escherichia coli.
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Rajput, Kamni, Bharti Chauhan, Ramesh Chandra Dubey, and Pankaj Kumar. "Antimicrobial Activity of Some Spices against Potential Probiotic Bacteria Isolated from Raw Sheep Milk." Current Journal of Applied Science and Technology 42, no. 29 (September 5, 2023): 1–11. http://dx.doi.org/10.9734/cjast/2023/v42i294201.
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Probiotics have several beneficial effects for human health. These probiotic bacteria in the human gut prevent harmful bacteria from adhering to the intestinal mucosa. Some studies report that the secondary metabolites of spices have an effect on common pathogenic bacteria, but their impact on beneficial probiotic bacteria is still not entirely understood. In this view, the present study aimed at the isolation of probiotic microorganisms from sheep milk, the screening of these organisms for a few probiotic characterizations, and an evaluation of the effect of several spices on the development of probiotic bacteria. A total of 26 bacterial strains were isolated from raw sheep milk samples. Five goat isolates, SMH12, SMH15, SMB16, SMB24, and SMB26, displayed acid and bile resistance, responses to simulated stomach and duodenum passage, and antibiotic susceptibility. An antibacterial activity study of these potential probiotic bacterial isolates revealed that bacterial isolate SMB16 was more effective at inhibiting the growth of both gram-positive and gram-negative pathogenic bacteria, viz., Escherichia coli (MTCC118), Staphylococcus aureus (MTCC7443), Pseudomonas aeruginosa (MTCC424), Listeria monocytogenes (MTCC657), and Salmonella typhimurium (MTCC733). Screening of the antimicrobial activity of different spices, such as garlic (Allium sativum), ginger (Zingiber officinale), and onion (Allium cepa), against selected probiotic bacterial isolates. The bacterial isolate SMB16 was tolerant of these spices, while other isolates were sensitive. This strain, SMB16, was identified as Enterococcus hirae by 16S rRNA gene sequence analysis.
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Rosana, Yeva, Lina Herliyana, Grady Krisandi, and Erike Anggraini Suwarsono. "Profile of multidrug-resistant bacteria causing urinary tract infections in inpatients and outpatients in Jakarta and Tangerang." Universa Medicina 42, no. 3 (November 20, 2023): 303–13. http://dx.doi.org/10.18051/univmed.2023.v42.303-313.
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Background Urinary tract infections (UTIs) caused by bacteria occupy the second highest rank of common infectious diseases in the world. Empirical use of antibiotics may give rise to multidrug-resistant (MDR) bacteria because of irrational prescription. Choice of antibiotics to treat UTIs is limited because of MDR bacteria. Thus, this study was conducted to investigate the bacterial antibiotic susceptibility patterns in inpatients and outpatients in Jakarta and Tangerang. Methods Bacterial isolates were obtained from midstream urine specimens from 43 inpatients and 43 outpatients with UTIs in Jakarta and Tangerang. Bacteria were isolated on blood and MacConkey agar media using colony count method. Isolate identification and their susceptibility patterns were performed using VITEK2 compact system according to manufacturers’ instructions. Data were analyzed using Chi-square test. Results A total of 89 bacterial isolates consisting of 15 bacterial species were successfully isolated from 86 specimens. Gram-negative bacteria were the most common etiology of UTIs in inpatients and outpatients. MDR bacteria were found in 52 of the 89 isolates. ESBL-producing E. coli was the most common MDR bacteria. ESBL-producing E. coli and other MDR bacteria showed good susceptibility to ertapenem, meropenem, amikacin, and tigecycline. There were no significant differences regarding the MDR bacterial count in inpatients and outpatients (p=0.521). Conclusion E. coli was found to be the most common MDR bacteria causing UTIs in inpatients and outpatients in Jakarta and Tangerang. Higher resistance to many antibiotics was found in MDR bacterial isolates in inpatients compared to outpatients. MDR bacteria in outpatient UTIs were highly resistant to commonly used antibiotics.
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Anggun Dwi Nur Annisa, Triastuti Rahayu, and Yasir Sidiq. "Growth of Endophytic Bacteria from Klutuk Banana Plant (Musa balbisiana Colla) with Inoculation Trial on Rice Plants (Oryza sativa)." Quagga: Jurnal Pendidikan dan Biologi 16, no. 1 (January 1, 2024): 20–27. http://dx.doi.org/10.25134/quagga.v16i1.53.
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Four potential bacteria have been isolated from Klutuk banana plant. They exhibited IAA production. Since the bacterial inoculation to plant should be performed in exponential phase of bacterial growth, information of the growth is crucial. This study aimed to analyze the growth rate of eight isolates and examine the effect of bacterial inoculation by initial trial on rice plants. For this purpose, four endophytic bacteria from Klutuk banana were cultured and examined using 600nm spectrophotometer. The growth of the isolates was calculated every two hours for 16 hours bacterial growth in the nutrient broth medium. The inoculation trial of bacteria to rice plants was conducted with three replicates. This inoculation was begun by germinating the rice seed and the radicle was observed. Then, the germinated rice seedlings were soaked in the bacterial suspension for 5 minutes in room temperature. The results showed that the exponential phase of all isolates was observed 6 hours after shaking. Inoculation using 6-hour-incubated K7 isolate significantly improved the length of root of rice plants. Additionally, this isolate improved the number of root and shoot length without significant difference. Thus, these results provide important information of the growth phase and inoculation effect new isolated endophytic and rhizosphere bacteria.
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Haque, MM, Mk Hasna, MI Khalil, KME Nabi, J. Ferthouse, and NR Paul. "Identification of plant growth promoting antagonistic bacteria against blast disease of rice." Bangladesh Journal of Nuclear Agriculture 37, no. 1 (November 21, 2023): 63–79. http://dx.doi.org/10.3329/bjnag.v37i1.69930.
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This study aimed to isolate and identify plant growth-promoting bacteria from rhizosphere of rice plants that exhibit antagonistic properties against Magnaporthe oryzae, the causal agent of blast disease. Bacteria were isolated from the surface of rice leaves, stems, and soil attached to the roots. The antagonistic activity of the isolated bacteria was assessed using a dual culture method, and identities were determined through 16S rDNA sequencing. To evaluate their growth-promoting abilities, assays were conducted to measure indole acetic acid (IAA) production, siderophore secretion, hydrogen cyanide (HCN) production, and phosphate solubilization. The results revealed that Bacillus subtilis, identified as BDISO_01R, exhibited antagonistic behavior and the maximum inhibition of M. oryzae (81.00%) was obtained by bacterial isolate BDISO-01R. Additionally, eight bacterial isolates demonstrated IAA production, sixteen isolates produced siderophores, and nine isolates displayed phosphate solubilization capability. This research sheds light on the diverse microbial arsenal that can potentially promote rice growth while combating blast disease. Bangladesh J. Nuclear Agric, 37(1): 63-79, 2023
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Lemenh, Yeshaneh Adimasu, Teshome Geremew Biru, Adinew Zewdu Chernet, and Feleke Belachew Lema. "Isolation and identification of protease‐producing bacteria from sludge and sediment soil around Adama, Ethiopia." Indonesian Journal of Biotechnology 26, no. 4 (December 31, 2021): 159. http://dx.doi.org/10.22146/ijbiotech.63987.
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Proteases are enzymes used in industries such the production and processing of detergents, food, leather, and silk. The aim of this study was to isolate and identify protease‐producing bacteria from a sludge disposal site and from sediments. Soil samples were collected separately from the selected area. Samples weighing 1 g were serially diluted and spread onto skim milk agar. A total of 16 bacteria species were isolated from the study samples. Four bacterial isolates showed high proteolytic activity and were selected for enzymatic study based on their zone of proteolysis. The isolates were identified based on biochemical tests. The results indicated that the isolated bacteria were E. coli (99.69%), Pseudomonas putrefaciens (Shewanella putrefaciens) (91.61%), Bacillus carboniphilus (92.78%), and Lysinibacillus sphaericus (98.4%). The crude protease enzymes produced by these bacterial isolates showed promising results for application in dehairing and destaining as detergent additives. Bacillus carboniphilus showed the best level of activity and was selected as the most potent protease‐producing bacteria for both dehairing and destaining ability. Soils from sludge disposal sites and sediments from around tannery wastes could be good sources from which to isolate alkaline protease‐producing bacteria.
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Sepriana, Citra, and Eti Sumiati. "Identifikasi Dan Uji Daya Hambat Isolat Bakteri Endofit Bunga Tanaman Cengkeh (Syzygium aromaticum L.) Terhadap Bakteri Patogen." Jurnal Penelitian Pendidikan IPA 6, no. 1 (January 29, 2020): 101. http://dx.doi.org/10.29303/jppipa.v6i1.340.
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This research was conducted to find out the capabilities of endophytic bacteria isolated from flowers of the clove plants in inhibiting the growth of bacteria Streptococcus mutans, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli and to identify endophytic bacteria that potensial to produce an antibacterial. Stages of this research include the isolation of endophytic bacteria from flowers of the clove plants, antibacterial test, and molecular identification based on 16S rRNA. Isolates of endophytic bacterial of clove plants flower produce 5 isolates, 4 isolate inhibited the bacteria S. aureus. Based on 16S rRNA molecular identification, endophytic bacterial isolates of clove plants flower which have inhibitory closely related to Bacillus amyloliquefasiens, Staphylococcus epidermidis 1034 MPA and Bacillus cereus JL.
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Clemetson, Lisa L., and Alton C. S. Ward. "Bacterial flora of the vagina and uterus of healthy cats." Journal of the American Veterinary Medical Association 196, no. 6 (March 15, 1990): 902–6. http://dx.doi.org/10.2460/javma.1990.196.06.902.
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Summary Bacterial culturing was conducted on samples from the reproductive tracts of 53 clinically healthy female cats. Aerobic bacteria were isolated from 52 of 53 vaginal swab samples and from 2 of 29 uterine swab samples. Anaerobic bacteria were detected in 4 of 30 vaginal and 1 of 29 uterine cultures. The aerobic bacteria included species of Acinetobacter, Actinomyces, Corynebacterium, Escherichia, Haemophilus, Klebsiella, Lactobacillus, Staphylococcus, and Streptococcus. Coagulase-negative Staphylococcus, Streptococcus canis, and E coli were the most common organisms and were isolated from 56%, 52%, and 44% of the vaginal samples, respectively. Anaerobes isolated from vaginal samples included 3 species of Bacteroides and 2 isolates of Peptococcus. The single uterine anaerobe isolate was a Lactobacillus sp. The number of bacterial species isolated from each vaginal culture ranged from I to 8 (mean, 3). The number of colony forming units tended to vary inversely with the number of bacterial species detected in each sample.
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Istifadah, Noor, Adelia Septiandini, Sri Hartati, and Fitri Widiantini. "Inhibition Effects of Culture Filtrates and Volatile Compounds of Antagonistic Microbes Isolated from Vermicompos and Compost Teas on the Growth of Alternaria solani Sor. in Vitro." CROPSAVER - Journal of Plant Protection 5, no. 2 (December 31, 2022): 98. http://dx.doi.org/10.24198/cropsaver.v5i2.43278.
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Alternaria solani Sor. is one of destructive pathogens in solanaceous plants including tomato. Bacteria and yeast isolated from water extract of organic matters are potential as biological control agents of plant pathogenic fungi. Mechanisms of antagonism of bacteria and yeast can be through antibiosis. This study was conducted to examine the abilities of culture filtrate and volatile compounds produced by antagonistic bacteria and yeast isolated from compost and vermicompost teas to inhibit the growth of A. solani in vitro. The experiments were arranged in randomized complete design with four replications. The culture filtrate experiment applied well diffusion method, while the volatile compound effect experiment used petri dish sandwich method. The results showed that the culture filtrates of four bacteria and three yeast isolates inhibited the growth of A. solani in vitro by 16.6-87.5%. The highest inhibition level was showed by KSB4 isolate (Bacillus subtilis), a bacterial isolate from cow manure compost tea. In the volatile compound effect experiment, the tested bacteria and yeast isolates inhibited the pathogen growth by 31.3-75.2%, with the highest inhibition was showed by KcB3, a bacterial isolate from vermicompost tea. The isolate that its culture filtrate and volatile compounds both showed high inhibition level (62.7% and 87.5%) on A. solani growth was KSB4 isolate (B. subtilis).
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IRAWATI, WAHYU, SEMUEL RIAK, NIDA SOPIAH, and SUSI SULISTIA. "Heavy metal tolerance in indigenous bacteria isolated from the industrial sewage in Kemisan River, Tangerang, Banten, Indonesia." Biodiversitas Journal of Biological Diversity 18, no. 4 (December 7, 2017): 1481–86. http://dx.doi.org/10.13057/biodiv/d180425.
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Irawati W, Riak S, Sopiah N, Sulistia S. 2017. Heavy metal tolerance in indigenous bacteria isolated from the industrial sewage in Kemisan River, Tangerang, Banten, Indonesia. Biodiversitas 18: 1481-1486. The bacterial study is a part of human calling in preserving the earth. Many indigenous bacteria isolated from heavy metal contaminated sites had resistance to heavy metal toxicity and could be used for heavy metal removal. The aims of this study were to isolate heavy metal-tolerant indigenous bacteria from the industrial sewage of Kemisan River in Tangerang, Banten, Indonesia. The potency of bacterial isolates to remove heavy metals was also determined. The heavy-metal tolerance was determined by measuring the minimum inhibitory concentration. The potency of bacterial isolate for removing heavy metals from the medium was determined by an atomic absorption spectrophotometer. The results showed that there were eight heavy metal-resistant bacteria isolated from Kemisan River with minimum inhibitory concentration ranging from 7 mM to 11 mM. Isolate PbSI1 was the highest lead tolerant bacteria, and also tolerant to copper and zinc. The isolate was able to remove 91.25% lead, 73.38% zinc, and 98.57% copper from medium supplemented with the mixture of these heavy metals. The addition of 9 mM of lead in the medium affected the morphological appearance of isolate colonies i.e PbSI1 and PbSI3 to become darker which might occur due to the survival mechanism of bacteria by absorbing the lead inside the cells. The finding of this study indicated that isolate PbSI1 was a promising bacterium, which could be further developed for heavy metal removal.
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Yusnia, Ella Dewi, Ida Bagus Wayan Gunam, and Nyoman Semadi Antara. "ISOLASI DAN SKRINING BAKTERI SELULOLITIK DARI BEBERAPA TANAH HUTAN DI BALI." JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI 7, no. 1 (April 1, 2019): 11. http://dx.doi.org/10.24843/jrma.2019.v07.i01.p02.
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Soil is one habitats of the cellulolytic bacteria. Soil containing manure and decayed wood is habitation for cellulolytic bacteria. This research is aimed to isolate and screen cellulolytic bacteria from the soil in Bali that has the potentials to degrade cellulose. The soil samples ware taken from forest in Gunaksa-Klungkung, Telaga-Karangasem, Sukahat-Karangasem, Gilimanuk-Jembrana, and Mangrove forests in Suwung-Denpasar. Each single cellulolytic bacteria colonies which grown on solid media containing carboxymethyl cellulose (CMC) were isolated. Screening of cellulolytic bacteria using congo red 0,1 %, staining bacterial isolates which were created clear zones in around the colony were selected. The isolation result are 67 isolated cellulolytic bacteria covered in five soils sample. Twenty-one bacterial isolates were producing cellulase enzymes with an indication of a clear zone in around the colony. Nine superior isolates have high cellulolytic index (CI), namely: G2-8 (5.41), G1-4 (4.86), G2-10 (4.5), G2-2 (3.64), M1-5 (3.10), G2-5 (3.03), M2-12 (2.72), G1-1 (2.38), and M1-1 (2.21). The obtained highest percentage of filter paper (Whatman No.1) degradation was 8.32% and the lowest was 2.48%.
Keywords: forest soil, isolation, cellulolytic bacteria, congo red
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Sidhiawan, Anak Agung Putu, Sukiman Sukiman, Sarkono Sarkono, Ernin Hidayati, and Bambang Fajar Suryadi. "Eksplorasi Potensi Bacillus spp. sebagai Bakteri Pemacu Pertumbuhan Tanaman di Hutan Primer Resort Kembang Kuning." Bioscientist : Jurnal Ilmiah Biologi 11, no. 2 (December 30, 2023): 1017. http://dx.doi.org/10.33394/bioscientist.v11i2.8403.
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Kembang Kuning resort is one of the Mount Rinjani National Park Resorts that have high biodiversity, so it has the potential for plant growth-promoting bacteria. Bacillus sp. is one species of bacteria that has great potential as a plant growth-promoting bacteria. The purpose of this study was to isolate and determine the ability of phosphate solubilization, nitrogen-fixing and anti-fungal activity of Bacillus spp. isolated from the Primary Forest of Kembang Kuning Resort, Gunung Rinjani National Park. This research is an exploratory study consisting of the isolation of Bacillus sp., phosphate solubilization test with spot inoculation method on Pikovskaya Agar media, qualitative nitrogen fixing test using Jensen's Nitrogen - Free media, and anti-fungal activity test against Fusarium sp. with dual culture method. There were 21 isolates of the genus Bacillus successfully isolated in this study. The phosphate solubilization test showed that 16 bacterial isolates could solubilize phosphate, where the highest phosphate solubilization index was produced by isolate P3.1 namely 0,52. A total of one isolate, namely isolate P3.1, can fix nitrogen on Jensen's Nitrogen-Free medium. In the anti-fungal test against Fusarium sp., three bacterial isolates were obtained that were able to inhibit the growth of Fusarium sp., where the highest antagonism was produced by isolate P2.8 which was 46,66%. Bacillus spp. isolated from Kembang Kuning Resort has potential as PGPB in terms of crop protection.
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De Fretes, Charlie Ester. "ISOLASI DAN KARAKTERISASI KEMAMPUAN BAKTERI ENDOFIT SORGUM MANIS FS501 SEBAGAI PENDUKUNG PERTUMBUHAN TANAMAN." EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi 5, no. 2 (December 31, 2020): 49–52. http://dx.doi.org/10.33019/ekotonia.v5i2.2107.
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This study aims to isolate endophytic bacteria of sweet sorghum plants and characterize their ability as plant growth promoting. The characters tested in this study were the ability of endophytic bacteria in N fixation, phosphate dissolving, and IAA production to be developed as biological fertilizer agents. Twenty-four isolates were isolated from the roots, stems and shoots of sweet sorghum. The results of bacterial DNA fingerprint screening showed that 11 groups of endophytic bacteria had different fingerprints. Isolates capable of N fixation were grown on LGI media and showed a change in the color of the medium. The nifH gene detection is also carried out to determine endophytic diazotrophic bacteria. Isolate bacteria that can dissolve inorganic phosphate were tested using Pikovskaya media. Testing the ability of isolates to produce IAA was carried out by adding Salkowski's reagent to the bacterial culture and measured quantitatively with a λ 530 nm spectrophotometer. The results showed that two endophytic bacterial isolates proved to be diazotrophic and three isolates were able to dissolve phosphate, while one isolate was able to produce IAA. PA2 isolate showed ability in all the characters tested, namely N fixation, phosphate solvent and IAA producer.
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Mawati, Sefi Desfeni, Esti Harpen, and Hilma Putri Fidyandini. "SKRINING BAKTERI TERMOFILIK POTENSIAL AMILOLITIK DARI SUMBER AIR PANAS WAY BELERANG KALIANDA LAMPUNG SELATAN." Journal of Aquatropica Asia 6, no. 1 (July 6, 2021): 1–7. http://dx.doi.org/10.33019/aquatropica.v6i1.2458.
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Thermophilic bacteria that produced amylase and protease have been isolated from Way Belerang hot spring, Kalianda, South Lampung. This research aims to screen and identify thermophilic bacteria that have the potential to produce thermostable amylase and protease enzymes.The research procedures included sampling, isolation of enzyme-producing thermophilic bacteria, a series of phenotypic and biochemical tests, and molecular identification by 16s rRNA. This study used 2 treatments, namely incubation temperature 37 and 50 ºC with 3 repetitions. The results showed that the optimum temperature for growth of thermophilic bacterial isolates and thermophilic bacterial isolates producing amylase enzymes was 50ºC. The bacteria isolate that had the best amylolytic enzyme activity was Isolate A.WB.50.1 with a diameter of the inhibitory zone was 15.44 mm. Isolate A.WB.50.1 has been identified by the species Pseudomonas stutzeri.
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Khristnaviera, Benedicta Yolanda, and Vincentia Irene Meitiniarti. "ISOLASI BAKTERI ASAM LAKTAT DARI KIMCHI DAN KEMAMPUANNYA MENGHASILKAN ZAT ANTI BAKTERI." Scripta Biologica 4, no. 3 (September 1, 2017): 165. http://dx.doi.org/10.20884/1.sb.2017.4.3.447.
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South Korea is famous for its fermented food called Kimchi, a traditional Korean food fermented from pickled vegetables with a mixture of spicy seasoning. Kimchi is now one of functional food products because of there are lactic acid bacteria that are probiotic and can produce bacteriocin compounds. These bacteriocin compounds may inhibit or have anti-bacterial activity. The purpose of this study was to obtain isolates of lactic acid bacteria from store-bought kimchi and homemade kimchi, to examine the antibacterial agent produced by lactic acid bacteria isolated from kimchi against Escherichia coli and Staphylococcus aureus bacteria. We conducted a Disk Diffusion Method to tests the bacteriocin activity, and data were descriptively analyzed. The results showed that eight isolates of lactic acid bacteria from store-bought kimchi and homemade kimchi homemade were able to inhibit the growth of tested bacteria, S. aureus and E. coli. Isolate D1 isolated from store-bought kimchi has largest inhibitory capability against S. aureus and E. coli; it has 16.00 mm and 17.33 mm inhibitory zone, respectively. Isolate B2 isolated from homemade kimchi has the most significant inhibitory ability against S. aureus and E. coli; it has 16.67 mm and 17.67 mm inhibitory zone, respectively. The lowest ability to form clear zone was found on isolate D2 isolated from homemade kimchi. The inhibitory zone of produce by strain D2 against S. aureus and E. coli were 7.67 mm and 8.67 mm, respectively.
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Ikerismawati, Senja. "BIOREMEDIASI Pb OLEH BAKTERI INDIGEN LIMBAH CAIR AGAR." Jurnal Biosilampari : Jurnal Biologi 1, no. 2 (June 29, 2019): 51–58. http://dx.doi.org/10.31540/biosilampari.v1i2.288.
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Bacteria isolated from ad environment contaminated with heavy metals are very potential as heavy metal bioremediation agents called indigenous bacteria. The purpose of this study was to isolate and identify indigenic bacteria that have the potential as Pb bioremediation agents in agar liquid waste and to analyze the ability of indigenic bacteria in reducing Pb. The research design used Completely Randomized Design (RAL) two factorial with variation of bacteria and eight days of treatment. Data were analyzed using two-way ANOVA with Duncan's advanced test. The results showed that there were eight isolates resulting from the isolation of agar liquid waste. The isolates of liquid waste indigen bacteria so that the most potential in reducing Pb were isolated H, E and F. The three isolates were able to reduce Pb in sterile agar liquid waste by 82.6%, 81.3% and 79.3% for eight days of treatment. The identification results using Microbact TM GNB 12A / B / E, 24 Identification Kits showed that H bacterial isolates were Bacillus alvei, E isolates were Bacillus pumilus species and F isolates were Bacillus lichenformis species
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Lombogia, Christian A., Max Tulung, Jimmy Posangi, and Trina E. Tallei. "Antibacterial Activities of Culture-dependent Bacteria Isolated from Apis nigrocincta Gut." Open Microbiology Journal 14, no. 1 (April 23, 2020): 72–76. http://dx.doi.org/10.2174/1874285802014010072.
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Introduction: Apis nigrocincta is a honeybee endemic to Mindanao island (the Philippines), Sangihe island (North Sulawesi, Indonesia) and Sulawesi mainland (Indonesia). The genus Apis is well known to have symbiont in their guts, which helps balance the microbiome in the gut and host health. Objective: The objective of this study was to determine whether the bacteria isolated from the gut of honeybee Apis nigrocincta produce metabolites with potential growth inhibition against Staphylococcus aureus and Escerichia coli, the bacteria which are important pathogens in humans and animals. Methods: Bacteria isolated from honeybee gut were cultured in MRSA and several isolates were purified for testing. The antibacterial activity test method used in this study was well diffusion agar. Pure isolates were grown on NB. The treatments given were heating and also neutralizing the supernatant from each isolate. Results: Five bacterial isolates were successfully isolated from honeybee gut and purified. The five isolates showed antibacterial activity against pathogenic bacterial strain indicators. The results of molecular identification showed that four of these isolates were Bacillus cereus and the other one was Staphylococcus arlettae. Neutralized supernatant showed strong activity on both indicator strains. The five isolates showed higher inhibition activity against S. aureus compared to E. coli. Conclusion: The finding of this research concluded that two bacterial strains, B. cereus and S. arlettae isolated from A. nigrocincta gut can be investigated further as agents which produce bioactive compounds that have potential as an antibacterial.
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Mahmood, Walaa Shakir. "The Effect of Bacteria on Dye." International Journal of Research in Science and Technology 12, no. 01 (2022): 19–30. http://dx.doi.org/10.37648/ijrst.v12i01.003.
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Microbiological and parasitological contamination of vegetables, water and soil in rural communities of a municipality was assessed. Samples were analyzed. Physical and chemical analyses were done. Background: Textile Industries are the major sector for social and economic perspective that discharge huge number of dye stuff containing recalcitrant compounds, pigments and dye etc. into the water. Aim: The aim of this study is to isolate and identify the textile dye degrading bacteria from textile effluents and examine the performance of bacteria that was able to completely decolorize the studied effluent after 3 weeks of incubation under agitation in an aerobic bioreactor. Methods and Materials: All the samples were collected and characterized. The isolated bacteria was characterized and identified by using cultural and biochemical techniques. Microbiological studied of contamination of water and soil in rural communities of a municipality was assessed. The decolorization of dye was measured using spectrophotometer of isolated bacteria. mixed isolates of species was selected for this study. Samples were analyzed. Physical and chemical analyses were done . Textile effluents (TEs) without correct treatment cause high environmental impact because they display several problems mainly due to toxicity and recalcitrance of dyestuffs. This study investigated the biosurfactant productions potentials of bacterial isolated from dye effluent. Enumeration, identification and characterization of the isolates were carried out using standard microbiological methods. The potential and ability to produce biosurfactants was determined using blood haemolytic tests, drop collapse and emulsification techniques. Results: gram negative and positive was the most effective decolorizers of textiles dyes than others bacterial species. A total of 16 organisms were isolated from different locations sampled, which are the predominant bacteria obtained from the three locations. Haemolysis results revealed that all the isolated bacterial strains exhibited haemolytic activity. The result of drop collapse test showed that all the isolated organisms had good collapsing ability, and all the isolated organism had positive oil spreading and emulsification ability. This study showed gram negative and positive species are potential biosurfactants producers and should be studied in greater details as strains improvement may enhance the activity of biosurfactants.
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Abid, Suhad A., Sarah Naji Aziz, Noor Al-Huda Ali A. H. Saeed, Shaimaa N. Mizil, Israa M. S. Al-Kadmy, Nadheema H. Hussein, Nadal Al-Saryi, Susan A. Ibrahim, and Jumaah D. Hussein. "Investigation of Virulence Factors in Microbial Organisms that Associated with Public Health Risk Isolates from Different Environmental Regions." Al-Mustansiriyah Journal of Science 33, no. 5 (February 25, 2023): 1–7. http://dx.doi.org/10.23851/mjs.v33i5.1303.
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Infectious diseases caused by infected tools in the environments are threaten to the safety and public health. Transmission sources of these infectious diseases are unknown, but it is thought that non-living materials called fomites, are the major source of acquired infections. Three hundred and one swabs were taken from different sources and cultured on blood agar to study heamolysis ability of isolated bacteria. In this study, MacConkey agar was used to isolate Gram-negative bacteria and Sabouraud agar (SDA) to isolate fungi. The biofilm formation test was done by Congo red plate assay. 41 (13.6%) bacterial isolates were obtained and (18.27%) of fungi were isolated on Sabouraud agar (SDA). Staphylococcus aureus was the more frequent bacterial species that isolated in this study. 29% of samples showed hemolysin activity on blood agar and 32%of the isolates were biofilm- producer. Results revealed that (7.9%) of Gram-negative bacteria harbored the fimH gene, (9%) harbored the icaA were Gram-positive and 6.3 % of fungal samples had HWP1 gene. Furthermore, (9.3%) from the total samples are bacterial samples harbored hla gene belong to Staphylococcus spp. Furthermore, (5.07%) of tested samples possessed hlyA gene were Gram-negative bacteria. We found in our study that infectious organisms can be transmitted from one individual to another by fomites responsible for acquired infection.
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Setyati, Wilis Ari, Muhammad Zainuddin, and Person Pesona Renta. "Pathogenic Assay of Probiotic Bacteria Producing Proteolytic Enzymes as Bioremediation Bacteria Against Vannamei Shrimp Larvae (Litopenaeus vannamei)." ILMU KELAUTAN: Indonesian Journal of Marine Sciences 22, no. 2 (June 6, 2017): 93–98. http://dx.doi.org/10.14710/ik.ijms.22.2.93-98.
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Application of bacteria in bioremediation of shrimp culture ponds is one of the methods used to clean internal pollutants. This study aimed to evaluate the pathogenicity of extracellular proteolytic enzyme produced by the probiotic bacteria as bioremediation bacteria on vannamei shrimp larvae culture. There were five probiotic bacteria, which were successfully isolated from the sediments served as substrate in mangrove area. The isolated bacteria were coded in number as 13, 19, 30, 33, and 36. Pathogenic bacteria Vibrio harveyi was used as positive control. Pathogenic assay was carried out in two different bacterial concentrations, i.e. 10⁸ and 10⁶ cells.mL-1. The results showed that the lowest survival rate (SR) of shrimp larvae in positive control V. harveyi was 53 and 65%. Whereas isolates with the highest SR value (100%) were obtained from bacteria coded as 13 and 30. Isolates no. 19, 33 and 36 had SR of more than 90%. Total plate count (TPC) data showed that the bacteria increased significantly at the end of the study with an average increase value of 24%. The smallest TPC value was shown by bacterial isolate no. 19, while the largest was obtained from the isolate no. 13. These results suggest that all probiotic bacteria were not pathogenic to the vannamei shrimp larvae. Keywords: aquaculture, shrimp, bioremediation, pathogenesis, vibrio.
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Meliah, Siti, Annisa Wahyu Hardiyanti, Ni’ma Haida, Gita Azizah Putri, and Erny Qurotul Ainy. "Penapisan Bakteri Penghambat Fusarium yang Diisolasi dari Cairan Kantung Semar (Nepenthes sp.)." Jurnal Ilmu Pertanian Indonesia 25, no. 4 (October 27, 2020): 627–35. http://dx.doi.org/10.18343/jipi.25.4.627.
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The genus Fusarium sp. is a pathogenic fungal for many cultivated plants. The bacteria isolated from monkey cup (Nepenthes sp.) fluid possess the ability to produce hydrolytic enzyme, such as chitinase which can be utilized to inhibit the growth of mycelia of pathogenic fungi. The aims of this study are to isolate bacteria from monkey cup liquid, to test their abilities to produce protease, chitinase, and cellulase, as well as their abilities to inhibit Fusarium. The bacteria were isolated using serial dilution method on Reasoner’s 2A agar medium. Enzymatic activities of bacterial isolates were determined by inoculating them on tested medium supplemented with casein protein, chitin, and cellulose, whereas their antifungal activities were assayed using a direct confrontation method between tested bacterial isolates and pathogenic fungal on Malt Extract Agar medium. Molecular identification of bacteria with antifungal activity was performed by analyzing the 16S rRNA gene sequences. Isolation process of bacteria from monkey cup fluid resulted in 99 bacterial isolates with the ability to produce either protease, chitinase, and/or cellulose enzymes. A total of 37 bacterial isolates were capable of producing at least two hydrolytic enzymes. Antifungal assay of those bacteria showed that as many as 25 isolates have the ability to inhibit the growth of Fusarium sp. Based on the analysis of 16S rRNA gene sequences revealed that those isolates were closely related to three Burkholderia species, namely B. arboris, B. contaminans, and B. rijonensis.
Keywords: antifungal, Burkholderia, chitinase, cellulaseN, epenthes, protease
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Hadi, Sapto Nugroho, Ida Widiyawati, Ahmad Fauzi, Prita Sari Dewi, and Yugi R. Ahadiyat. "Identification of Potential Biofertilizer and Bioremediator Bacteria from Upland Soil Based on 16s rDNA Sequence Analysis." PLANTA TROPIKA 11, no. 2 (September 18, 2023): 133–40. http://dx.doi.org/10.18196/pt.v11i2.12744.
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The long-term presence of synthetic pesticides on agricultural land can lead to a decline in soil fertility. Synthetic pesticides inhibit the activity of essential enzymes in the soil and suppress beneficial microbial populations for plants. One potential approach to mitigate the extent of contamination caused by synthetic pesticides involves the utilization of indigenous pesticide-resistant bacteria. Several upland soil bacteria from Banyumas Regency, Central Java Province, Indonesia, were successfully isolated from a previous study. The isolated bacteria have the potential to be developed as pesticide bio-remediators and biofertilizers. The bacterial isolates are expected to have characters that support plant growth through their ability to provide dissolved phosphate. However, the potential bacterial isolates need to be identified by molecular approaches. This study was conducted to identify bacterial isolates of GT2, SR1, SW1, and PA1 by 16S rDNA sequencing analysis. The results showed that isolate GT2 was placed within a group of reference strains of Bacillus proteolyticus, isolate SR1 was placed within a group of B. paramycoides, isolate SW1 was set within a group of B. albus, and isolate PA1 was placed within a group of Acidovorax delafieldii. The genetic distance of isolate GT2 and B. B. proteolyticus, isolate SR1 and B. paramycoides, isolate SW1 and B. albus were 0.0000 each, and isolate PA1 and A. delafieldii were 0.0061.
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Halim, Rashidah Abd, Nor'Aishah Hasan, and Kogeethavani Ramachandran. "Screening of Endophytic Bacteria as Biocontrol Agents Against Bacteria Leaf Blight (Xanthomonas oryzae)." HAYATI Journal of Biosciences 27, no. 3 (July 1, 2020): 215. http://dx.doi.org/10.4308/hjb.27.3.215.
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Bacterial leaf blight (BLB) is one of major threats in rice production as it can cause 100% yield loss. Concern on the environment and human health has led an attempt to replace existing methods of chemical control and avoid extensive use of bactericides by using endophytic bacteria. The present study was conducted to screen and characterize bacteria isolated from different sources that has potential as antagonistic bacteria against Xanthomonas oryzaepv. oryzae (Xoo), the causal agent of bacterial leaf blight of paddy. Two hundred and thirty-three endophytic bacteria were successfully isolated from roots and leaves from paddy field. Only 17 endophytic bacterial isolates showed positive antagonistic activity indicated by inhibition zone around bacterial colony against Xoo on nutrient agar plate with 2 endophytic isolates (BCA 3 and BCA 12) showed highest inhibitory effect with 35±0.00 mm in diameter. Molecular identification by 16S rRNA amplification successfully identified the antagonistic endophytic bacteria as Pseudomonas fluorescensand Geobacillus thermoparaffinivorans. Findings in this study revealed the biocontrol abilities of isolated endophytes as an excellent option to be used by agriculture sectors to have sustainable environment.
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Ropiatningsuari, Nika, Suryo Wiyono, and Suryahadi Suryahadi. "Skrining Bakteri Dekomposer Sebagai Penghilang Bau Kotoran Ayam." Bumi Lestari Journal of Environment 18, no. 1 (February 1, 2018): 19. http://dx.doi.org/10.24843/blje.2018.v18.i01.p03.
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The decomposition of chicken excreta produce odorous gases, that case environmental pollution. One of alternative technique to reduce the odorous gases is by applying specific bacteria. The aim of this study was to obtain bacterial isolates that capable of reducing NH3 and H2S production and odors. Decomposer bacterial candidates were isolated from chicken manure, guano, and peat soil. Selection of isolates of oxidizing bacteria is carried out using selective media. All isolates that were found from chicken manure, guano, and peat soil tested for hypersensitive reaction on tobacco and hemolysis on blood agar. The isolated bacteria that showed negative HR and HL responses than used for a further experiment. Five isolates of bacteria which reduce odors based on organoleptic test were WiK 15, TnK 7, WiGu 11, CGu 7 and MaGa 5. NH3 and H2S from decomposition chicken excreta were analyzed by spectrophotometric and colorimetric method. The average of total NH3 production from seven days observation showed ranged level from 1.09 ppm to 1.77 ppm, while total H2S gas production ranged from 15.05 to 16.57 ppm. Bacterial isolate CGU 7 showed make lowest total production of NH3.
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Rahman, MM, AA Khan, and AM Akanda. "Biological Control of soft rot bacteria of onion in Bangladesh." Bangladesh Journal of Scientific and Industrial Research 56, no. 4 (December 29, 2021): 231–40. http://dx.doi.org/10.3329/bjsir.v56i4.57196.
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An investigation was conducted to search antagonistic bacteria as biological control agents of soft rotting bacterial pathogen of onion (Allium cepa L.) in vitro and in storage. Antibacterial activity of previously isolated 91 bacterial isolates was tested in vitro against onion soft rot bacteria Burkholderia cepacia O-15. Two isolates namely, R-15 and E-37 were found antagonistic against onion soft rot bacteria. Isolate R-15 was identified as the genus Bacillus and the isolate E-37 to Lactobacillus sp. Isolate R-15 proved to be a strong antagonist against onion soft rot bacteria was selected for bio-control of onion in storage. That was also effectively reduces the soft rot disease of onion in storage condition. Percentage of disease reduction (PDR) due to treatment with antagonistic bacteria was 72.4% compared to untreated control. It is therefore suggested that this isolate could be exploited as biocontrol agent for onion soft rot in Bangladesh. Bangladesh J. Sci. Ind. Res.56(4), 231-240, 2021
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Aljanaby, Ahmed Abduljabbar Jaloob, and Israa Abduljabbar Jaloob Aljanaby. "Prevalence of aerobic pathogenic bacteria isolated from patients with burn infection and their antimicrobial susceptibility patterns in Al-Najaf City, Iraq- a three-year cross-sectional study." F1000Research 7 (July 30, 2018): 1157. http://dx.doi.org/10.12688/f1000research.15088.1.
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Background: Burn infections are one of the most common serious illnesses caused by pathogens, mainly by both gram-negative and gram-positive bacteria. The aim of this study was to detect of the prevalence of multi-drug resistant and extended-spectrum β-lactamase-producing (ESBL) bacteria isolated from inpatients with burn infection and the antimicrobials sensitivity patterns of all bacterial isolates during three years. Methods: This cross-sectional study was performed in Al-Najaf Central Hospital in Al-Najaf City, Iraq from January 2015 to December 2017. A total of 295 burns swabs were collected from hospitalized patients with burn infection. All grown bacterial isolates were identified by standardized microbiological tests. Antimicrobials susceptibility testing was done using the disc diffusion method. Multi-drug, extensive-drug and pan-drug resistant bacteria and extended-spectrum β-lactamase-producing bacteria were determined according to standardized methods and guidelines. Results: Of the 295 burn swabs, 513 different bacteria strains were isolated. Pseudomonas aeruginosa was the most common bacteria with 142 isolates (27.6%) followed by methicillin resistance Staphylococcus aureus 106 isolates (20.6%), while Staphylococcus typhi was the least common bacteria with only 17 isolates (3.3%). 323 (63%) different bacterial strains were isolated from patients who stayed in hospital for 15 days. Most bacterial isolates were resistant to most antimicrobials with high percentages. Out of the 513 bacterial isolates; only 33 isolates (6.4%) were resistant to imipenem 10µg and 464 isolates (90.4%) were multi-drug resistant, 20 isolates (14%) were extensive-drug resistant and 17 isolates (3.3%) were pan-drug resistant. Pseudomonas aeruginosa was the most common ESBL-producing bacteria (51 isolates-35.9%). Conclusions: There was a high prevalence of multi-drug resistant bacteria in burn infection in Al-Najaf hospital. Pseudomonas aeruginosa was the most common multi-drug resistant bacteria, and the most common of ESBL bacteria causing burn infection over the three years.
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Pattani, Vivek B. "Characterization of Plant Growth-Promoting Activity of Bacteria Isolated from Forest and Coastal Regions of Saurashtra, Gujarat, India." Bioscience Biotechnology Research Communications 15, no. 1 (March 25, 2022): 144–51. http://dx.doi.org/10.21786/bbrc//15.1.22.
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The haphazard application of chemical fertilizers and pesticides causes tremendous damage to ecosystems and all biota. One of the most effective ways to tackle the threat is to use biofertilizer. Plant growth promoting bacteria (PGPB) are an important bacterial source for microbial fertilizers that can boost agricultural yields by encouraging plant growth. Bacterial isolates isolated from Saurashtra region, Gujarat, India were analysed for their capability to solubilize inorganic 'P' from tri calcium phosphate and production of indole acetic acid (IAA) quantitatively by bacterial. Production of ammonia, siderophore and hydrogen cyanide (HCN) by selected bacteria isolates was analysed. Biochemical characterization of selected bacterial isolates was done using Vitek 2 Compact system. Isolate GFS15C2 showed highest amount of phosphate solubilization, followed by isolate GFS07C1 and GFS01C1. Bacterial isolate GFS15C2 produced highest amount of IAA. All bacterial isolates were able produce ammonia. Eight bacteria isolates were be to produce HCN. Siderophore was produced by 14 bacterial isolates. In biochemical characterization all the bacterial isolates were able to use D-glucose. Based on biochemical characters clustering of bacteria isolates was done using Paleontological statistics software package for education and data analysis(PAST). Using cluster analysis by euclidean distance method based on biochemical characterization isolates GFS16C2 & SCS12C3 was found to have distinct characters than other isolates. The present study attempts to characterize PGPB which could be harnessed to improve plant growth. Several phosphate solubilizers and IAA producers also showed production of siderophores and HCN which suggests that these organisms do possess biocontrol ability. These PGPB microbial inoculants can be utilized to improve agricultural systems or as an alternate means of environmentally friendly plant disease biocontrol.
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RINA, NURFITRIANI, NI PUTU RATNA AYU KRISHANTI, ALINA AKHDIYA, and ARIS TRI WAHYUDI. "Penapisan Bakteri Filosfer Penghasil Senyawa Bioaktif Anti Xanthomonas oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri pada Padi." Jurnal Sumberdaya Hayati 2, no. 1 (November 14, 2016): 19–24. http://dx.doi.org/10.29244/jsdh.2.1.19-24.
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Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the important diseases in rice crops in Indonesia. The disease is difficult to be controlled since it attacks the rice plant at different growth stages such as tillering, flowering and ripening. One of the alternatives that could be used to control the disease is by using phyllosphere bacteria as the biocontrol agents. This study aims to isolate, characterize and screen the rice phyllosphere bacteria producing bioactive compounds against Xoo. Phyllosphere bacteria isolated from healthy leaves of rice var. Ciherang by using 4 different media obtained 285 bacterial isolates which were consisted of the 65 isolates of King’s B agar, 86 isolates of Nutrient agar, 81 isolates of Luria-Bertani agar, and 53 isolates of Trypticase Soy agar media. Antagonist test using double layer method showed 58 isolates of phyllosphere bacteria produced bioactive compounds that inhibited the growth of Xoo. Pathogenicity test agaist rice leaf revealed 18 bacterial isolates did not perform their potencies as pathogenic bacteria. Among the 18 non-phytopathogenic bacterial isolates, 14 isolates belong to Gram-positive bacteria and 4 isolates belong to Gram-negative bacteria. Five isolates among Gram positive bacteria were predicted as Bacillus genera.
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Septiani, Reti, Rahmad Lingga, and Verry Andre Fabiani. "ISOLASI DAN KARAKTERISASI BAKTERI RESISTEN CD DARI KAWASAN PENAMBANGAN TIMAH." CONSERVA 1, no. 1 (June 30, 2023): 1–13. http://dx.doi.org/10.35438/conserva.v1i1.183.
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Bangka Belitung Province is one of the largest tin producing area in Indonesia. One of the mining sites located on the Sampur Beach, Kebintik Village, Central Bangka Regency. Tin mining activities can increase heavy metals harmful to the environment, such as Cadmium (Cd). This research aimed to measure Cd levels in sediment and seawater, to isolate and identify Cd resistant bacteria from sediment. Cd metal was analyzed using Atomic Absorption Spectrophotometer (AAS). The average Cd metal level in seawater is 0,0080 ppm and in sediment is 0,0086 ppm. Bacteria were isolated using Zobell 2216E media with the spread plate method. The isolated Bacteria were tested for resistance to Cd with concentration of 20 ppm, 50 ppm, 80 ppm, 100 ppm, and 120 ppm. From 16 bacterial isolates that have been obtained, 7 isolates were resistant to all concentrations, 1 isolate was resistant to 50 ppm, 1 isolate was only resistant to 20 ppm, and 7 isolates were not resistant. Based on the results of the analyzed, the seven isolates of resistant bacteria have similarity with Halomonas sp. (R2 and R3), Neisseria sp. (R4), Cupriviadus sp. (R5), Bacillus sp. (R11), Alcaligenes sp. (R12), and Roseobacter sp. (R13).
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Melizah, Ardesy, Syarif Husin, and Syifa Alkaf. "Identification of Lactic Acid Bacteria Isolate From Fermentation Food Bekasam." Bioscientia Medicina : Journal of Biomedicine and Translational Research 2, no. 1 (January 13, 2018): 16–23. http://dx.doi.org/10.32539/bsm.v2i1.34.
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Bekasam is a local food from South Sumatera Indonesia, which is obtained through fermentation of fish. Previous study has shown that fermented food contained Lactic Acid Bacteria (LAB) bacteria, such as Lactobacillus plantarum, Lactobacillus sp, Pediococus sp and Weissella sp. The study was conducted to identify isolate LAB from bekasam. Bekasam contained fish, rice, and salt with a certain ratio. Further isolation of isolated LAB isolate, then performed PCR for bacterial analysis. Isolate 1 showed alleged bacteria Staphylococcus sp, non pathogen, while isolate 2 leads to Lactobacillus sp. The PCR results show the suitability of the bands formed between the Lactobacillus standard and the isolates.
Keywords: bacteria, lactobacillus, acid
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KHALILA, RUHUL, Lenni Fitri, and SUHARTONO SUHARTONO. "Isolation and Characterization of Thermophilic Bacteria as Cellulolytic Enzyme Producer from the Hot Spring of Ie Seuum Aceh Besar, Indonesia." Microbiology Indonesia 14, no. 1 (August 11, 2020): 4. http://dx.doi.org/10.5454/mi.14.1.4.
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Cellulase enzymes can be isolated from thermophile bacteria obtained from the hot spring Ie Seuum, Aceh Besar. This research aimed to recover and characterize the isolates morphologically and biochemically followed by determination of the thermophile bacterial isolates potential as cellulolytic enzyme producers,. The sampling method in this research was conducted by a purposive sampling at temperature of 70 oC, 60 oC and 50 oC. Isolation of thermophilic bacteria was carried out on nutrient agar (NA) media. There were four isolates of thermophilic bacteria isolated recovered at 70 oC, five isolates at 60 oC, and seven isolates at 50 oC. Of the 18 isolates obtained, 15 of them were able to produce cellulase enzymes. Cellulase enzyme production can be determined by the presence of clear zones around bacterial colonies on CMC media after addition of 1% congo red drops and wash with 1 M NaCl. The highest five Cellulolytic Index (CI) values were obtained from isolates ISB75; ISB64; ISB52; ISB54; ISB56 that were 1.23; 2.22; 1.39; 1.59; 1.10, respectively. Biochemical tests carried out on 5 isolates with the highest cellulolytic index values showed that the bacterial isolate were suspected to be from the genera of Bacillus sp.
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Kurniawan, Ardiansyah, Suci Puspita Sari, Euis Asriani, Andi Kurniawan, Abu Bakar Sambah, and Asep Awaludin Prihanto. "Molecular Identification of Cellulolytic Bacteria From Mangrove Sediment at Tin Minning Region In West Bangka." International Journal of Applied Biology 3, no. 1 (June 29, 2019): 7. http://dx.doi.org/10.20956/ijab.v3i1.5848.
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Cellulose as an abundant source of glucose in Indonesia requires acceleration of decomposition utilizing cellulolytic bacteria. Cellulolytic bacteria can be obtained from the isolation of mangrove organic matter, such as sediments. Muntok Sub-district is one of the regions with the most tin mining in West Bangka Regency also has mangroves in the coastal area. Exploration of cellulolytic bacteria in mangroves with different environmental characteristics encourages researchers to find new bacterial strains that produce cellulase enzymes with new properties. Thirteen isolates were successfully isolated from three locations. Tembelok mangrove sediments produced Seven bacterial isolates, Peltim Mangrove samples produced three isolates and from Sukal Mangrove three isolates were obtained. Seven isolates showed clear zones in the Lugol test and three isolates including were gram-positive bacteria. Molecular test with 16S rRNA analysis showed TBL1 isolate has 85% similar identity of Vibrio parahaemolyticus strain HY3 and TBL2 isolate has 98% similar identity of Bacillus amyloliquefaciens strain HS8. Bacillus amyloliquefaciens potential to further study as cellulose degrading bacteria for feed ingredients.
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Kusumasari, Wiwi, Ana Indrayati, and Lukito Mindi Cahyo. "AKTIVITAS PROTEASE EKSTRASELULER BAKTERI YANG DIISOLASI DARI LIMBAH CAIR INDUSTRI TAHU." Jurnal Insan Farmasi Indonesia 6, no. 1 (May 31, 2023): 134–43. http://dx.doi.org/10.36387/jifi.v6i1.1266.
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Tofu waste that is discharged into the aquatic environment has a negative impact on the quality of the water around the tofu liquid waste disposal site. Industrial enzyme production is currently dominated by proteases. Proteases in the pharmaceutical field support the absorption of proteins in the digestive tract, as active cosmetic ingredients. This study aims to isolate, identify protease-producing bacteria from tofu wastewater, determine the total protein content and enzyme activity index of 5 isolates of protease-producing bacteria. Tofu wastewater bacteria were isolated with Skim Milk Agar medium. 5 bacterial isolates were selected which produced the widest clear zones, different colony characters, identified by morphological tests, gram staining tests, and biochemical tests. Five isolates were made into a bacterial suspension isolated from crude protease extract and the protease level was determined using the Bicinchoninic Acid method, and the protease activity was tested. Five bacterial colonies showed that colony 1 and colony 2 included Gram positive bacteria, colonies 3, 4 and 5 included Gram negative bacteria. The value of total protein content in the tofu industrial wastewater from the five colonies respectively: 219.93; 833,267; 421,267; 81,267; and 38.6 µg/µL. The highest proteolytic activity test was in sample 2 of 2.13.
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Zhang, Peipei, József Baranyi, and Mark Tamplin. "Interstrain Interactions between Bacteria Isolated from Vacuum-Packaged Refrigerated Beef." Applied and Environmental Microbiology 81, no. 8 (February 6, 2015): 2753–61. http://dx.doi.org/10.1128/aem.03933-14.
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ABSTRACTThe formation of bacterial spoilage communities in food is influenced by both extrinsic and intrinsic environmental factors. Although many reports describe how these factors affect bacterial growth, much less is known about interactions among bacteria, which may influence community structure. This study investigated interactions among representative species of bacteria isolated from vacuum-packaged (VP) beef. Thirty-nine effectors and 20 target isolates were selected, representing 10 bacterial genera:Carnobacterium,Pseudomonas,Hafnia,Serratia,Yersinia,Rahnella,Brochothrix,Bacillus,Leuconostoc, andStaphylococcus. The influence of live effectors on growth of target isolates was measured by spot-lawn agar assay and also in liquid culture medium broth using live targets and effector cell-free supernatants. Inhibition on agar was quantified by diameter of inhibition zone and in broth by measuring detection time, growth rate, and maximum population density. A number of interactions were observed, with 28.6% of isolates inhibiting and 4.2% promoting growth. The majority ofPseudomonasisolates antagonized growth of approximately one-half of target isolates. TwoBacillusspp. each inhibited 16 targets. Among lactic acid bacteria (LAB),Carnobacterium maltaromaticuminhibited a wider range of isolates compared to other LAB. The majority of effector isolates enhancing target isolate growth were Gram-negative, includingPseudomonasspp. andEnterobacteriaceae. These findings markedly improve the understanding of potential interactions among spoilage bacteria, possibly leading to more mechanistic descriptions of bacterial community formation in VP beef and other foods.
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Riana, Heidy, Supono Supono, and Agus Setyawan. "MOLECULAR IDENTIFICATION AND LOCAL ISOLATE BACTERIAL ACTIVITY TEST AS BIOCONTROL CANDIDATES TO TACKLE Vibrio Spp INFECTIONS AT VANNAMEI SHRIMP CULTIVATION (Litopenaeus vannamei) IN EAST LAMPUNG." e-Jurnal Rekayasa dan Teknologi Budidaya Perairan 9, no. 2 (February 27, 2021): 1131. http://dx.doi.org/10.23960/jrtbp.v9i2.p1131-1142.
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Pacific white shrimp (Litopenaeus vannamei) is a widely cultivated type of shrimp with high economic value. One of the obstacles in pacific white shrimp cultivation is an infectious disease, especially Vibrio spp. This study aims to identify bacteria of biocontrol candidate against Vibrio spp. This research was conducted by the Laboratory of Fisheries and Marine, Faculty of Agriculture, University of Lampung and the laboratory of PT. Indolab Utama, West Jakarta, from September 2019 to January 2020. Bacteria of Biocontrol candidate were isolated from traditional shrimp pond water in Purworejo Village, Pasir Sakti District, East Lampung Regency. Water samples were taken from the inlet, plot, and outlet. The bacterial screening was carried out by testing the antagonism of bacterial isolates isolated from water samples with several Vibrio bacteria, namely V. harveyi, V. algynoliticus, and V. parahemolyticus on Sea Water Complete (SWC) medium. The bacterial isolates with the highest inhibitory activity were then identified morphologically, biochemically, and molecularly by 16s rRNA. The results showed that from those 10 isolates was found 1 bacterial isolate from water samples. Only one pond plot isolate (P1) was found that had inhibitory activity against V. harveyi and the size was 21 mm. The morphological test results showed the colonies were round, flat edges, convex elevation, cream colored, and included in the negative gram bacteria group. The results of biochemical tests showed that the bacteria were motile. Meanwhile, the molecular test results with 16s rRNA showed that the P1 bacteria were 99% similar to Aeromonas caviae isolates, which were found potential as biocontrol candidates because they can inhibit the growth of V. harveyi bacteria, which was characterized by the formation of a clear zone around the paper disk.
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Acevedo-Barrios, Rosa, Angela Bertel-Sevilla, Jose Alonso-Molina, and Jesus Olivero-Verbel. "Perchlorate-Reducing Bacteria from Hypersaline Soils of the Colombian Caribbean." International Journal of Microbiology 2019 (February 17, 2019): 1–13. http://dx.doi.org/10.1155/2019/6981865.
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Perchlorate (ClO4−) has several industrial applications and is frequently detected in environmental matrices at relevant concentrations to human health. Currently, perchlorate-degrading bacteria are promising strategies for bioremediation in polluted sites. The aim of this study was to isolate and characterize halophilic bacteria with the potential for perchlorate reduction. Ten bacterial strains were isolated from soils of Galerazamba-Bolivar, Manaure-Guajira, and Salamanca Island-Magdalena, Colombia. Isolates grew at concentrations up to 30% sodium chloride. The isolates tolerated pH variations ranging from 6.5 to 12.0 and perchlorate concentrations up to 10000 mg/L. Perchlorate was degraded by these bacteria on percentages between 25 and 10. 16S rRNA gene sequence analysis indicated that the strains were phylogenetically related toVibrio,Bacillus,Salinovibrio,Staphylococcus, andNesiotobactergenera. In conclusion, halophilic-isolated bacteria from hypersaline soils of the Colombian Caribbean are promising resources for the bioremediation of perchlorate contamination.
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M. Saleh, G. "ISOLATION AND CHARACTERIZATION OF UNIQUE FRUCTOPHILIC LACTIC ACID BACTERIA FROM DIFFERENT FLOWER SOURCES." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 51, no. 2 (April 26, 2020): 508–18. http://dx.doi.org/10.36103/ijas.v51i2.977.
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This study was aimed to investigate Lactic acid bacteria (LAB) that contain an important group of bacterial strains such as fructophilic lactic acid bacteria that usually isolated from fructose rich niches. These groups of bacteria have been considered as probiotics, especially in animal applications. In this study these bacteria were isolated from 6 Plant sources (flowers) such as: Convolvulus arvensis, Hibiscus rosa-sinensis , Nerium oleander, Rosa rugosa, Tagetes erecta and Zinnia elegans. The bacterial isolates were identified by morphological and molecular methods through the detection of 16SrRNA gene. Carbohydrate fermentation profile as well as their antibacterial activity using the dual culture overlay assay was also detected. The results revealed that 5 types of different lactic acid bacteria were isolated included: Fructobacillus fructosus, Lactobacillus kunkeei, Enterococcus durans, Enterococcus faecium , and Lactobacillus brevis. These isolates showed variety in fermentation of carbohydrates. Also, all isolates exhibit inhibition effect against pathogenic bacteria (Staphylococcus auresو Pseudomonas aeruginosa) except Enterococcus faecium which showed no effect it.
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Rai, Pooja, Sonali Das, Suresh Kamble, and Namita Raytekar. "Isolation, Identification and Characterization of Feather degrading Bacteria from Feather Dumping Sites." Research Journal of Biotechnology 17, no. 6 (May 25, 2022): 109–15. http://dx.doi.org/10.25303/1706rjbt1090115.
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The present study was aimed to isolate, identify and characterize bacteria showing high keratinolytic activity from poultry wastes. Keratinase producing microorganism has the ability to degrade keratin rich substrate. The bacteria producing keratinase enzyme was isolated from the chicken feather. The isolates were identified morphologically and biochemically. Based on the proteolytic activity, isolates were tested for their ability to degrade the feather. Bacteria were grown in the basal media with feather meal broth and showed feather degrading capacity. The bacterial strains were identified as Bacillus sp., Klebsiella sp. and Escherichia coli. The degradation of feather was observed by these bacteria.
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Erdoğmuş, Sevim Feyza, Uğur Cengiz Erişmiş, and Cevdet Uğuz. "Isolation and identification of lactic acid bacteria from fermented meat products and evaluation of their antimicrobial effect." Czech Journal of Food Sciences 39, No. 4 (August 29, 2021): 289–96. http://dx.doi.org/10.17221/222/2020-cjfs.
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In this study, 30 lactic acid bacteria (LAB) were isolated from 20 different fermented meat products obtained from the Afyonkarahisar province (Turkey). Molecular identification of these isolates was performed by amplification of the 16S rDNA gene using the polymerase chain reaction (PCR) method. LAB isolated from 'sucuk' (spicy and fermented sausage) samples were identified as Enterococcus faecalis (2 isolates), Enterococcus durans (1 isolate), Lactobacillus sakei (3 isolates), Lactobacillus curvatus (2 isolates), Weissella viridescens (3 isolates), Weissella cibaria (2 isolates) and Weissella hellenica (1 isolate). LAB, isolated from salami samples, were identified as W. viridescens (1 isolate), E. durans (3 isolates), Leuconostoc mesenteroides (4 isolates), Carnobacterium maltaromaticum (1 isolate), Macrococcus caseolyticus (1 isolate). Also, LAB, isolated from sausages samples, were identified as E. faecalis (1 isolate), E. durans (4 isolates), Lactobacillus plantarum (1 isolate). Both agar spot and agar well diffusion assay methods were used to determine the antimicrobial activity of the LAB isolates. Isolates of LAB showed higher antimicrobial activity against Listeria monocytogenes ATCC 19115, Staphylococcus aureus ATCC 25923, Klebsiella pneumoniae NRRL B 4420, Pseudomonas aeruginosa ATCC 11778, Streptococcus faecalis NRRL B 14617 than against Escherichia coli ATCC 35218 and Bacillus subtilis NRS 744. Results showed that the LAB isolates produced antimicrobial substances that have a potential for different industrial and biotechnological uses.