Journal articles on the topic 'Bacillus proteus'
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1
JP, Omorodion Nnenna, and Wisdom John. "Effects of Wrapping Materials on the Microbial Population of Steam Bean Pudding (Moimoi)." Journal of Food Technology Research 9, no. 1 (April 7, 2022): 80–93. http://dx.doi.org/10.18488/jftr.v9i1.2955.
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The effects of wrapping materials on the microbial population of Moimoi were assessed. A total of thirty samples of wrapped Moimoi of plastic, waterproof and aluminum foil was collected from food vending sites located in Choba. The samples were taken to the laboratory and analyzed using standard microbiological methods. The total prevalence of bacteria that occurred from foil Moimoi were Micrococus (35%), Bacillus (25%) , Staphylococcus (20%) and Proteus (20%). The total prevalence of bacteria obtained on plastic Moimoi were Staphylococcus (27%), Bacillus subtilis (23%) Proteus sp (23%), Micrococcus sp (13%) and Bacillus cereus (14%). Total prevalence obtained from nylon Moimoi were Staphylococcus (27%), Bacillus cereus (23%), Proteus (18%), Micrococcus (18%) and Bacillus subtilis (14%). The mean total bacteria count, Staphylococcus count and coliform count obtained ranged from 9.8 x103 - 1.35 x103 cfu/g. The result of the study further shows that most of the gram-positive isolates such as Staphylococcus sp, Micrococcus sp, Bacillus subtilis and Bacillus cereus were resistant to antibiotics of ceftriaxone, cloxacillin, Augmentin, ceftazidime and cefuroxime while the gram-negative bacteria isolated Proteus sp was resistance to Augmentin and cefuroxime but susceptible to gentamycin, erythromycin and cefuroxime. Contamination of these wrapped Moimoi samples is due to poor personal hygiene and sanitation among food handlers. To prevent outbreak of food poisoning, users of this wrapping materials should wash wrapping materials with clean water before using and food in these wrapping materials should be consumed immediately to reduce multiplication of microorganisms.
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Ainul, Asri, M. Hasbi, and Eko Purwanto. "Isolation and Identification of Biosurfactant Producing Bacteria From Workshop Wastewater." Ilmu Perairan (Aquatic Science) 9, no. 1 (March 30, 2021): 31. http://dx.doi.org/10.31258/jipas.9.1.p.31-37.
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Automotive workshop activities produce oil ills that may pollute waters around the workshop area. The oil-polluted water may inhabit biosurfactant producing bacteria that are able to degrade the oil. A study aimed to isolate and identify the bacteria has been conducted from July to September 2020. The bacteria samples were sampled from workshop wastewater at Kubang Raya street KM 2,5 Pekanbaru, Riau Province and sampling were conducted three times. The bacteria were isolated using TSB (Tryptone Soy Broth) and TSA (Tryptone Soy Agar) media and were identified by using biochemical methods. Results showed that there were seven types of bacteria, namely Providencia, Proteus, Acinetobacter, Bacillus, Aeromonas, Proteus and Serratia. The Emulsification index of Providencia was 38.8%, Proteus 50%, Acinetobacter 48.8%, Bacillus 52,1%, Aeromonas 47,6%, Proteus 54,7% and Serratia 48,8%. Data obtained from this study showed that all of the identified bacteria are able to produce biosurfactants.
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AGBABIAKA, Toyin Olayemi, and Blessing Olubunmi OLOFINTOYE. "Microbial Diversity in Water and Biofilm Samples from Well Sources in Ilorin Metropolis, Nigeria." Notulae Scientia Biologicae 11, no. 1 (March 21, 2019): 56–62. http://dx.doi.org/10.15835/nsb11110337.
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The aim of the study was to investigate the microbial populations of water and biofilm samples from seven hand dug wells in Ilorin, Nigeria, which are for private and public use. Physicochemical parameters such as pH, turbidity, total dissolved solids, temperature, conductivity, dissolved oxygen and biological oxygen demand were determined. Biofilm growths were induced from water samples obtained from selected wells. Twenty bacteria isolated were identified using morphological and biochemical characteristics as well as molecular methods and includes Shigella sonnei, Shigella dysenteriae, Micrococcus luteus, Bacillus sphaericus, Salmonella enteritidis, Proteus mirabilis strain IK-MB4-518F, Bacillus licheniformis strain RH104, Bacillus subtilis, Erwinia sp., Proteus vulgaris, Yersina sp., Bacillus cereus, Staphylococcus aureus, Serratia marcescens, Pseudomonas aeruginosa strain GS1, Pseudomonas aeruginosa strain 218B, Staphylococcus epidermidis, Vibrio sp., Escherichia coli and Bacillus pasteurii. Total bacterial count ranged from 1.8×104-1.53×106 cfu/ml. Water samples from these wells are therefore not potable and some of the isolates are potential health hazards to human population.
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Yusuf UA, Ekeleme IK, Owuna EJ, Ochai SS, and Obiekezie SO. "Effects of spent hydrocarbon on bacteria population." International Journal of Scholarly Research in Science and Technology 2, no. 1 (January 30, 2023): 001–9. http://dx.doi.org/10.56781/ijsrst.2023.2.1.0033.
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This study aimed at the effect of spent hydrocarbon contamination on microbial population in soil. Standard microbiological methods were used to determine the total heterotrophic count hydrocarbon utilization, isolation and identification of bacterial and effect of pH and spent hydrocarbon concentration on bacterial. The total heterotrophic bacterial count (THB) ranges from 6.2 ± 0.13 x106 to 3.2± 0.10 x106 cfu/g. The total hydrocarbon utilizing bacterial count ranges from 3.2 ± 0.13 x106 to 1.2± 0.10 x106 cfu/g. The bacterial isolated were Bacillus subtilis, Pseudomonas flourescens, Klebsiella aerogenes and Proteus hauseri. It was observed that Bacillus subtiliis had the highest occurrence from location E (50.0%). Pseudomonas flourescens from location A and B (33.3%) and Proteus hauseri had the highest occurrence from location C (66.6%). The effect of pH on bacterial growth rate analyzed showed that Bacillus subtilis had the highest turbidity at pH 6.5 (0.511 ± 0.15 nmm), Klebsiella aerogenes had the highest turbidity at pH 7.5 (0.233 ± 0.33nm), Pseudomonas flourescens was at pH 6.5 (0.723 ± 0.61 nm) and Proteus sp recorded highest turbidity at pH 6.5 (0.373 ± 0.22nm) followed by pH 5.5 (0.237 ± 0.19 nm). The effect of spent hydrocarbon concentration showed that Bacillus subtilis recorded highest turbidity at 10% concentration (0.744 ± 0.03 nm), Klebsiella aerogenes recorded highest at 10% concentration (0.321 ± 0.21 nm), Pseudomonas flourescens was at 10% concentration (0.887 ± 0.23 nm) and Proteus sp recorded highest turbidity at 10% concentration (0.378 ± 0.13 nm). From this study it was observed that indigenous bacterial had the ability to utilized the spent hydrocarbon if the pH of the soil is regulated.
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Makut, Makwin Danladi, Toyosi Michelle Adebayo, and Jibril Egwu Owuna. "Bioremediation of soil polluted with spent lubricating oil using bacteria isolated from soil in Abuja Metropolis." BIOMED Natural and Applied Science 02, no. 03 (December 10, 2022): 33–40. http://dx.doi.org/10.53858/bnas02033340.
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Background: The presence of spent hydrocarbon in soil is a serious problem to the environment hence study on bioremediation of soil polluted with auto-mechanic oil in Abuja Metropolis was carried out. Methods: A total of twenty (20) soil samples were collected, bacteria were isolated from the contaminated soil and identified using standard microbiological methods. The spent hydrocarbon utilization was determined using Atomic Adsorption UV Spectrometer. Results: The total viable count of the bacteria was 1.07 x 106 from Apo Mechanic village, 1.10 x 106 from Utako Mechanic workshops, 0.40 x 106 from Gwarinpa Mechanic workshops and 2.04 x 106 from Area one Mechanic workshops. The percentage occurrence of bacteria from Apo Mechanic village was Enterobacter species 40.0%, Pseudomonas synxantha 60.0%, Bacillus zanthoxyli 40.0% and Proteus vulgaris 20.0%. Utako Mechanic workshops were Enterobacter kobei 20.0%, Pseudomonas synxantha 40.0%, Bacillus zanthoxyli 20.0% and Proteus vulgaris 40.0%. Gwarinpa Mechanic workshops were Pseudomonas synxantha 20.0% and Bacillus zanthoxyli 20.0%. Area one Mechanic workshops were Enterobacter kobei 40.0% and Pseudomonas synxantha 40.0%. The effect of days on utilization of spent hydrocarbon showed that Pseudomonas synxantha had highest utilized of spent hydrocarbon 19.55mg/ml after 21 days. The effect of pH on utilization of spent hydrocarbon show that at pH 7.5, Enterobacter kobei, Bacillus zanthoxyli and Proteus vulgaris species had the highest utilization of spent hydrocarbon ranging from 5 9.33mg/ml-12.70mg/ml. Effect of temperature on utilization of spent hydrocarbon showed that at 28OC Enterobacter kobei, Pseudomonas synxantha, Bacillus zanthxyli and Proteus vulgaris had the highest utilization of spent hydrocarbon ranging from 5.51mg/ml- 11.11mg/ml. the bacteria isolated from the contaminated soil have the ability to utilized the hydrocarbon if the soil is amended with some mineral element as shown in this study. Conclusion: In conclusion bacteria isolates effectively bioremediated the automechanic oil polluted soil with a reduction of hydrocarbon pollutants.
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Patel, Binal R., MT Panchal, AJ Dhami, RA Mathakiya, and BB Bhanderi. "Bacterial Isolates from the Genital Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Buffaloes." INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY 15, no. 02 (November 25, 2019): 56–59. http://dx.doi.org/10.21887/ijvsbt.15.2.15.
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The study was carried out on 50 vaginal secretions/aspirates/discharge samples collected aseptically using syringe and pipette method from infertile (anestrus; endometritic, n = 6 each) buffaloes of villages nearby Anand and healthy cyclic (n = 5; proestus, estrus, metestrus, diestrus) as well as 3, 6 and 9 month pregnant (n = 6 each) buffaloes of University farm to identify the vaginal microorganisms based on routine cultural examination. In all 117 bacterial isolates were recovered from all 50 vaginal samples (100 %) of 35 buffaloes during different physio-pathological status. The bacteria isolated from vaginal mucus/aspirates of buffaloes during the follicular phase comprised Corynebacterium spp. as the most predominant isolate (28.57%) followed by E. coli, Bacillus Spp., Staphylococcus spp., Streptococcus spp., Salmonella spp., Proteus spp., and vaginal yeast, whereas during the luteal phase, the most predominant bacteria were E. coli (23.33%) followed by Corynebacterium spp., Bacillus spp., Staphylococcus spp., Streptococcus spp., and Klebsiella spp. In acyclic buffaloes, the most predominant bacteria isolated were Corynebacterium spp. (21.43%) Bacillus spp., Micrococcus spp., Pseudomonas, Staphylococcus spp., Streptococcus spp., E. coli and Salmonella spp., whereas the endometritic buffaloes evinced the most predominant bacterial isolates as Corynebacterium spp. and E. coli (20.00% each) followed by Bacillus spp., Salmonella, Proteus spp., Staphylococcus spp., Streptococcus spp., and Klebsiella spp. The major bacteria isolated during the entire period of pregnancy were E. coli, Micrococcus, Corynbacterium Spp., Bacillus spp., Staphylococcus spp. and Proteus. This study concludes rich bacterial diversity in the vagina of buffaloes during different physio-pathological status.
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Abdul-Jabbar, Amna M., Nehia N. Hussian, Hamdoon A. Mohammed, Ahmed Aljarbou, Naseem Akhtar, and Riaz A. Khan. "Combined Anti-Bacterial Actions of Lincomycin and Freshly Prepared Silver Nanoparticles: Overcoming the Resistance to Antibiotics and Enhancement of the Bioactivity." Antibiotics 11, no. 12 (December 10, 2022): 1791. http://dx.doi.org/10.3390/antibiotics11121791.
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Bacterial drug resistance to antibiotics is growing globally at unprecedented levels, and strategies to overcome treatment deficiencies are continuously developing. In our approach, we utilized metal nanoparticles, silver nanoparticles (AgNPs), known for their wide spread and significant anti-bacterial actions, and the high-dose regimen of lincosamide antibiotic, lincomycin, to demonstrate the efficacy of the combined delivery concept in combating the bacterial resistance. The anti-bacterial actions of the AgNPs and the lincomycin as single entities and as part of the combined mixture of the AgNPs–lincomycin showed improved anti-bacterial biological activity in the Bacillus cereus and Proteus mirabilis microorganisms in comparison to the AgNPs and lincomycin alone. The comparison of the anti-biofilm formation tendency, minimum bactericidal concentration (MBC), and minimum inhibitory concentration (MIC) suggested additive effects of the AgNPs and lincomycin combination co-delivery. The AgNPs’ MIC at 100 μg/mL and MBC at 100 μg/mL for both Bacillus cereus and Proteus mirabilis, respectively, together with the AgNPs–lincomycin mixture MIC at 100 + 12.5 μg/mL for Bacillus cereus and 50 + 12.5 μg/mL for Proteus mirabilis, confirmed the efficacy of the mixture. The growth curve test showed that the AgNPs required 90 min to kill both bacterial isolates. The freshly prepared and well-characterized AgNPs, important for the antioxidant activity levels of the AgNPs material, showed radical scavenging potential that increased with the increasing concentrations. The DPPH’s best activity concentration, 100 μg/mL, which is also the best concentration exhibiting the highest anti-bacterial zone inhibition, was chosen for evaluating the combined effects of the antibiotic, lincomycin, and the AgNPs. Plausible genotoxic effects and the roles of AgNPs were observed through decreased Bla gene expressions in the Bacillus cereus and BlaCTX-M-15 gene expressions in the Proteus mirabilis.
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Taiwo Stephen Okanlawon, Stella Mojisola Adeyemo, and Ibukun Sylvester Agbaje. "Isolation and identification of microorganisms associated with Jollof rice sold at Bukateria in Obafemi Awolowo University, Ile -Ife, Osun State, Nigeria." GSC Biological and Pharmaceutical Sciences 22, no. 1 (January 30, 2023): 178–85. http://dx.doi.org/10.30574/gscbps.2023.22.1.0274.
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This study assessed the microbiological quality of Jollof rice by isolating, identifying and characterizing the microorganisms associated with jollof rice. Six replicate samples were collected from different locations in Obafemi Awolowo University campus, Ile-Ife, Osun-State, Nigeria. Maconkey, Nutrient and Potatoes Dextrose agars were used for the isolation and determination of microbial load. Standard morphological and biochemical tests were carried out for the identification and characterization of isolates. A total of 10 bacteria and 10 fungal species were isolated. The total bacterial count ranged from 3.6×103cfu/g to 1.54×105cfu/g, while the total fungal count ranged from 1.04×104sfu/g to 3.0×105sfu/g. The organisms presumptively identified with the percentage occurrence include; Staphylococcus saprophiticus (20%), Proteus vulgaris (10%), Bacillus subtlis (10%), Proteus mirabilis (10%), Micrococcus varians (10%), Bacillus licheniformis (10%), Bacillus cereus (10%), Bacillus polymyxa (10%), Micrococcus luteus (10%) for bacteria isolates and Aspergillus niger (30%), Aspergillus oryzae (30%) and Aspergillus candidus (40%) for fungi isolates. Good personal hygiene, proper sanitation practice and the use of clean utensils during food preparation are recommended to avoid food poisoning and spoilage.
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Salih, N. A. "Antibacterial effect of nettle (Urtica dioica)." Al-Qadisiyah Journal of Veterinary Medicine Sciences 13, no. 1 (June 30, 2014): 1. http://dx.doi.org/10.29079/vol13iss1art270.
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The antibacterial activity of aqueous and 95% ethanol extracts of nettle leaf was tested against some Gram-negative and Gram-positive bacteria isolated from hospitalized patients by the agar well diffusion method. Staphylococcus aureus, Escherichia coli, Klebsiella spp., Bacillus Subtillus, Proteus spp. Salmonella spp. and Pseudomonas spp. were used. The results indicate that both extracts showed different antibacterial activities which were in favor of ethanolic extract because of more solubility of active ingredient in ethanol than in water. Staphylococcus aureus, Bacillus Subtillus, and Salmonella spp. showed the highest susceptibility to nettle extracts antibacterial effect, while E coli, Pseudomonas and Proteus were less susceptible. The only clear resistant bacteria isolate was Klebsiella spp.
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Raimondi, Stefano, Gloria Spampinato, Laura Ioana Macavei, Linda Lugli, Francesco Candeliere, Maddalena Rossi, Lara Maistrello, and Alberto Amaretti. "Effect of Rearing Temperature on Growth and Microbiota Composition of Hermetia illucens." Microorganisms 8, no. 6 (June 15, 2020): 902. http://dx.doi.org/10.3390/microorganisms8060902.
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The potential utilization of black soldier fly (Hermetia illucens) as food or feed is interesting due to the nutritive value and the sustainability of the rearing process. In the present study, larvae and prepupae of H. illucens were reared at 20, 27, and 33 °C, to determine whether temperature affects the whole insect microbiota, described using microbiological risk assessment techniques and 16S rRNA gene survey. The larvae efficiently grew across the tested temperatures. Higher temperatures promoted faster larval development and greater final biomass but also higher mortality. Viable Enterobacteriaceae, Bacillus cereus, Campylobacter, Clostridium perfringens, coagulase-positive staphylococci, Listeriaceae, and Salmonella were detected in prepupae. Campylobacter and Listeriaceae counts got higher with the increasing temperature. Based on 16S rRNA gene analysis, the microbiota of larvae was dominated by Providencia (>60%) and other Proteobateria (mainly Klebsiella) and evolved to a more complex composition in prepupae, with a bloom of Actinobacteria, Bacteroidetes, and Bacilli, while Providencia was still present as the main component. Prepupae largely shared the microbiota with the frass where it was reared, except for few lowly represented taxa. The rearing temperature was negatively associated with the amount of Providencia, and positively associated with a variety of other genera, such as Alcaligenes, Pseudogracilibacillus, Bacillus, Proteus, Enterococcus, Pediococcus, Bordetella, Pseudomonas, and Kerstersia. With respect to the microbiological risk assessment, attention should be paid to abundant genera, such as Bacillus, Myroides, Proteus, Providencia, and Morganella, which encompass species described as opportunistic pathogens, bearing drug resistances or causing severe morbidity.
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Sharma, Probir Kumar, Zakya Sultana Jui, Tahsin Khan, Tahmina Islam, and Mihir Lal Saha. "Biotechnologically Important Enzyme Producing Indigenous Bacteria Isolated from Fruit and Vegetable Wastes Samples Collected from different Local Markets." Plant Tissue Culture and Biotechnology 32, no. 1 (June 27, 2022): 31–41. http://dx.doi.org/10.3329/ptcb.v32i1.60470.
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Enzyme from bacterial sources is much stable and obtained cheaply. Amylases and proteases are among the most important enzymes. During this study, indigenous amylase and protease producing bacteria were isolated from common decayed fruits and vegetable wastes viz. Papaya, Brinjal, Cucumber, Potato and Snake Gourd. Bacterial load ranged in between 0.67 × 109 and 9.06 × 109 cfu/g on NA and 1.50 × 109 and 7.00 × 109 cfu/g on PYG agar medium. Maximum mean bacterial load on both NA (6.19 ± 2.60 × 109 cfu/g) and PYG agar (4.54 ± 1.03 × 109 cfu/g) were observed in decayed papaya. A total of 113 bacterial isolates were primarily isolated. Considering better amylase and protease activity 16 isolates were selected for detailed study. The starch hydrolysis ratio (SHR) of the isolates ranged in between 1.25 ± 0.37 and 2.47 ± 0.23 while casein hydrolysis ratio (CHR) ranged in between 2.35 ± 0.12 and 6.44 ± 1.16. The highest SHR was 2.47 ± 0.23 found in Bacillus sp. of snake gourd and the highest CHR was 6.44 ± 1.16 found in Bacillus subtilis also from snake gourd. Out of the 16 isolates 13 were Gram positive and 3 were Gram negative. Gram positive isolates were identified as Bacillus acidocaldarius (4), B. firmus (2), B. lentus (4), B. subtilis (2) and B. alcalophilus (1). Gram negative isolates were identified as Edwardsiella hoshinae, Proteus myxofaciens and P. mirabilis. Six isolates having higher SHR and CHR were authenticated through molecular identification and were identified as Chryseobacterium sp. S29.2, Bacillus sp. X8, Bacillus sp. strain GA1B, Bacillus sp.TdEND26, Bacillus subtilis strain BPA28 and Bacillus subtilis BAB-881. The maximum amylase (61.33 ± 2.14 U/ml) and protease (56.91 ± 0.23 U/ml) production were observed in Bacillus sp. TdEND26 rd. In case of co-production, the highest amount of amylase (54.13 ± 1.23 U/ml) protease (81.80 ± 4.54 U/ml) production was observed in Bacillus sp. TdEND26 at 24 hrs. Plant Tissue Cult. & Biotech. 32(1): 31-41, 2022 (June)
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Bakun, Y., L. Ulko, and O. Nechiporenko. "Effect of probiotics Bacillus coagulans and Bacillus megaterium on intestinal microbiota of piglets." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 23, no. 104 (December 23, 2021): 136–40. http://dx.doi.org/10.32718/nvlvet10422.
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Following the ban on the use of antibiotics in animal feed in January 2006 in the EU due to fears and the emergence of antibiotic-resistant bacteria (ARBs), combined with increased consumer demand for safe food of animal origin, new strategies have been proposed to address diarrhea in piglets. occurs after weaning from sows. Accordingly, probiotics are used to create a healthy gut microbiota, improve health, well-being and productivity at all stages of pork production. Probiotics have been identified by FAO as living microorganisms that benefit animal health when taken in the right amounts. Recently, however, due to increased interest and increased research on probiotics, the definition has been expanded to include microorganisms and their fermentation products. Probiotics are defined as mixtures of known viable microbes in equivalent amounts that modify the host's microflora to benefit its health. Particularly reliable are the strains of Bacillus megaterium and Bacillus coagulans, which are able to withstand extreme environments, such as high levels of acid in the stomach, and this makes them particularly effective in eliminating gastric distress and other diseases. The aim of the study was to determine the effect of probiotic strains of microorganisms on the microbiota of the gastrointestinal tract of piglets. Addition to the diet of piglets Bacillus megaterium has a positive effect on the reproduction and accumulation of lactobacilli, helps to suppress opportunistic pathogens from the family Enterobacteriaceae (Proteus, Citrobacter, Enterobacter, Klebsiella). Probiotic strains of Bacillus coagulans, Bacillus megaterium, reduce the number of opportunistic pathogens from the family Escherichia coli, which has hemolytic activity, compared with the control group where Escherichia coli with hemolytic activity was isolated. The number of opportunistic pathogens from the family Enterobacteriaceae (Proteus, Citrobacter, Enterobacter, Klebsiella) in the feces of piglets of the experimental group fed Bacillus coagulans and Bacillus megaterium was 101, which is lower than in the control group – 1×102.
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Meng, Jiang Ping. "Design and Synthesis of Novel Antimicrobial Benzimidazolium Compounds." Advanced Materials Research 554-556 (July 2012): 1686–89. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.1686.
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In this research, a series of novel benzimidazolium compounds were designed, synthesized and evaluated for antibacterial and antifungal activities against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Bacillus proteus, and two fungi, Candida albicans and Aspergillus fumigatus. All of the synthesized compounds exhibited significant activity against the evaluated bacteria. The structures of these novel compounds were measured by 1H NMR, IR and mass spectrum.
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Pavilonis, Alvydas, Algirdas Baranauskas, Ligita Puidokaitė, Žaneta Maželienė, Arūnas Savickas, and Raimondas Radžiūnas. "Antimicrobial activity of soft and purified propolis extracts." Medicina 44, no. 12 (June 16, 2008): 977. http://dx.doi.org/10.3390/medicina44120122.
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Objective. To evaluate the antimicrobial activity of soft and purified propolis extracts. Study object and methods. Antimicrobial activity of soft and purified propolis extracts was determined with reference cultures of Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 33499, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 12459, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 8035, and fungus Candida albicans ATCC 60193. Microbiological tests were performed under aseptic conditions. Minimum inhibitory concentration (MIC) – the highest dilution of preparation (the lowest concentration of preparation) that suppresses growth of reference microorganisms – was determined. Results. Concentration of phenolic compounds in soft propolis extract that possesses antimicrobial activity against gram-positive (Staphylococcus aureus, Enterococcus faecalis) and gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis) is 0.587±0.054 mg and 0.587±0.054–0.394±0.022 mg (P>0.05) and in purified propolis extract – 0.427±0.044 mg and 0.256±0.02 mg (P>0.05). Klebsiella pneumoniae is most resistant to soft propolis extract when the concentration of phenolic compounds is 1.119± 0.152 mg and to purified propolis extract when the concentration of phenolic compounds is 1.013±0.189 mg (P>0.05). Spore-forming Bacillus subtilis bacteria are more sensitive to soft and purified propolis extracts when the concentration of phenolic compounds is 0.134±0.002 mg and 0.075±0.025 mg, respectively, and Bacillus cereus – when the concentration is 0.394±0.022 mg and 0.256±0.02 mg (P>0.05). Sensitivity of fungus Candida albicans to soft and purified propolis extracts is the same as Bacillus subtilis. Encapsulated bacterium Klebsiella pneumoniae is most resistant to antimicrobial action of soft and purified propolis extracts as compared with gram-positive Staphylococcus aureus and Enterococcus faecalis bacteria (P<0.05), gram-negative Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis (P<0.05), sporeforming Bacillus subtilis and Bacillus cereus bacteria (P<0.05), and fungus Candida albicans (P<0.05). There is no statistically significant difference between antimicrobial effect of soft propolis extract and purified propolis extract on gram-positive bacteria, gram-negative bacteria, spore-forming bacteria, encapsulated bacteria, and Candida fungus. Conclusions. Soft and purified propolis extracts possess antimicrobial activity. They could be recommended as natural preservatives in the manufacture of pharmaceutical products.
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Jurkštienė, Vilma, Alvydas Pavilonis, Daiva Garšvienė, Algirdas Juozulynas, Laimutė Samsonienė, Dalia Daukšienė, Konstancija Jankauskienė, Genovaitė Šimonienė-Kazlauskienė, and Edgaras Stankevičius. "Investigation of the Antimicrobial Activity of Rhaponticum (Rhaponticum Carthamoides D.C. Iljin) and Shrubby Cinquefoil (Potentilla Fruticosa L.)." Medicina 47, no. 3 (March 22, 2011): 24. http://dx.doi.org/10.3390/medicina47030024.
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The aim of the study was to determine antimicrobial activity of rhaponticum and shrubby cinquefoil extracts. Material and Methods. Ethanol extract from the leaves of rhaponticum (Rhaponticum carthamoides D.C. Iljin) and shrubby cinquefoil (Potentilla fruticosa L.) was produced at the Department of Food Technology, Kaunas University of Technology. The antimicrobial activity of the viscous extract or rhaponticum and shrubby cinquefoil was evaluated using standard microorganism cultures (bacteria Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 33499, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 12459, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 8035 and fungi Candida albicans ATCC 60193). The minimum inhibitory concentration (MIC) of the examined preparations was determined. Results. Both studied preparations – rhaponticum (Rhaponticum carthamoides D.C. Iljin) and shrubby cinquefoil (Potentilla fruticosa L.) – demonstrated similar antimicrobial activity. The highest sensitivity to the studied preparations was observed in microbes with eukaryotic cell structure: Candida albicans, which is a fungus, and a spore-forming prokaryotic bacterium, Bacillus cereus. The highest resistance was observed in Escherichia coli and Klebsiella pneumoniae. Conclusions. The studied preparations – viscous extracts of rhaponticum and shrubby cinquefoil – are substances with antimicrobial activity against gram-positive (Staphylococcus aureus and Enterococcus faecalis) and gram-negative (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus mirabilis) bacteria, spore-forming bacteria (Bacillus subtilis and Bacillus cereus), and fungi (Candida albicans).
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Habiba, Ume, Jaweria Nisar, Muhammad Akram Choohan, Syed Muhammad Ali Shah, Zonaira Nisar, and Imtiaz Mustafa. "Antibacterial Activity of Tris NaCl and PBS Buffer Protein Extract of Cassia fistula, Saccharum officinarum, Albizia lebbeck and Cymbopogon citrates Against Bacterial Strains." Dose-Response 19, no. 1 (January 1, 2021): 155932582199223. http://dx.doi.org/10.1177/1559325821992239.
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Medicinal plants are gaining popularity over synthetic medicines because antibiotic resistance demands the alternative source of medication. In the present research, the crude protein extraction of 4 medicinal plants Cassia fistula, Saccharum officinarum, Albizia lebbeck and Cymbopogon citrates was carried out. Crude protein extraction was done by 2 different buffers i.e. Tris NaCl buffer and PBS buffer. Protein confirmation was done by Bradford assay in the spectrophotometer. Antibacterial potential was checked and compared against Escherichia coli, Bacillus subtilis, Neisseria gonorrhoea, Bacillus cereus and Proteus mirabilis. Antibacterial assay was performed by disc diffusion method, agar well method and zones of inhibition were calculated. The study results indicated that Tris NaCl extracts’ antimicrobial potential is higher than that of the PBS buffer. On disc diffusion method the Tris NaCl buffer extracts of Cymbopogon citrates showed maximum zone of inhibition 11 mm and 9 mm against Bacillus subtilis and Bacillus cereus respectively and control chloramphenicol showed maximum zone of inhibition 26 mm against Bacillus subtilis. Cassia fistula showed maximum zone of inhibition of 7 mm against Bacillus cereus while Saccharum officinarum and Albizia lebbeck didn’t show the any antibacterial activity. On the other hand, Protein extracts from PBS buffer didn’t show zone of inhibition against any bacteria. Only Albizia lebbeck showed minute zone of inhibition against Neisseria gonorrhea. On well diffusion method, Cassia fistula Tris NaCl protein extract showed the maximum zone of inhibition 20 mm and 18 mm against Proteus mirabilis and Bacillus subtilis respectively. While Albizia lebbeck PBS protein extract showed the maximum zone of inhibition 19 mm and 17 mm against Bacillus subtilis and Bacillus cereus. The results revealed that the protein extract of Albizia lebbeck, Cymbopogon citrates and Cassia fistula can be used tosynthesize antimicrobial drugs to treat the bacterial infections.
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Mahammed, N., R. T. Abagai, and Y. Mohammed. "Isolation and Characterization of Bacteria Species Related to Food Contact Surfaces in Selected Cafeterias in a Tertiary Institution Campus in ABU, Zaria, Nigeria." Journal of Applied Sciences and Environmental Management 26, no. 7 (July 31, 2022): 1245–50. http://dx.doi.org/10.4314/jasem.v26i7.9.
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The study was carried out to isolate and characterize bacterial species from selected cafeterias in tertiary institution campus in Ahmadu Bello University Zaria, Nigeria. A total of ten samples were selected for analysis which include; spoons, plates, forks, chopping boards, worktops, tables, hands, washing water, plate rinsing water1 and plate rinsing water2. The bacteriological analysis was carried out using the nutrient agar (NA) media, which was prepared by dissolving 28g of the NA powder in 1litre of distilled water, autoclaved and allowed to cool. The growth of the microorganisms were then observed and counted per ml. The identified species include; Staphylococcus aureus, Staphylococcus epidermis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis and Bacillus anthracis. The food contact surfaces analyzed revealed that Chopping board has the highest bacterial load with 28 x 105, followed by Hand 27 x 105, Worktop 20 x 105, Table 18.8 x 105, Washing water 16 x 105, Plate 13.6 x 105, Fork 12 x 105, Spoon 7.2 x 105, plate rinsing water1 6 x 105, plate rinsing water2 5 x 105. The frequency of occurrence of the isolated organisms include; Staphylococcus aureus 3(10.3), Staphylococcus epidermis 9 (31.1), Escherichia coli 4(13.8), Klebsiella pneumonia 6(20.7), Pseudomonas aeruginosa 3(10.3), Proteus mirabilis 2(6.9) and Bacillus anthracis 2(6.9). It was observed that Staphylococcus epidermis have the highest incidence of occurrence, Bacillus anthracis and Proteus mirabilis have the least incidence of occurrence.
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M�rsdorf, Gerhard, and Heinrich Kaltwasser. "Ammonium assimilation in Proteus vulgaris, Bacillus pasteurii, and Sporosarcina ureae." Archives of Microbiology 152, no. 2 (July 1989): 125–31. http://dx.doi.org/10.1007/bf00456089.
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Wang, Xiao Xiao, Luo Jun Gong, Wei Han, and Qian Qian Yan. "The Microbial Diversity Analysis of Constructed Wetland Wastewater Treatment in Village." Applied Mechanics and Materials 295-298 (February 2013): 1098–103. http://dx.doi.org/10.4028/www.scientific.net/amm.295-298.1098.
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This paper uses the method of 16SrRNA to analyze the microbial diversity in wetland wastewater treatment of Wuhan galaxy ecological agriculture Co., LTD . Using the method of 16SrRNA, we can get the molecular identification of the strain from screening , determine the 16SrRNA gene sequence and analyzes the homology of the corresponding sequence of related bacteria. The result shows that 32 strains are Bacillus subtilis, two strains are Bacillus licheniformis, six strains are Staptococcus cohnii, one strain is Staphylococcus epidermidis, one strain is Pseudomonas synxantha, and one strain is People umber bacillus in the first wetland. 36 strains are Bacillus subtilis, three strains are Bacillus licheniformis, one strains are Staptococcus cohnii, one strain is Proteus mirabilis, and two strain Staphylococcus epidermidis are the second wetland. The main strain in the two-stage wetland both are Bacillus subtilis.
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Atikpo, E., J. E. Agori, E. R. Iwema, A. Michael, and L. O. Umukoro. "Non-Linear Error Functions Approach to Kinetic Study of Arsenic Removal from Soils using Proteus mirabili and Bacillus subtilis." Nigerian Journal of Environmental Sciences and Technology 2, no. 1 (March 2018): 130–36. http://dx.doi.org/10.36263/nijest.2018.01.0065.
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Kinetics of arsenic (As) removal by Proteus mirabilis and Bacillus subtilis were studied using the non-linear error functions approach. The two microorganisms (from soils samples obtained from a contaminated site in Amaonye-Ishiagu in Ebonyi State of Nigeria) were cultured and employed to deplete the metal ion from the contaminated soils. The experimental data were studied with four kinetic models namely pseudo first order, pseudo second order, simple Elovich, and intraparticle diffusion models using some non-linear error functions of root mean square error (RMSE), standard error of experiment (SEE), average relative error (ARE) and normalized standard deviation (NSD). Data from removal by both organisms were best described with pseudo first order model indicated by the ARE of minimum value of -1.8728 for Proteus mirabilis, and -2.1208 for Bacillus subtilis. These showed that the removal mechanism was reaction controlled as chemisorption was the rate limiting step.
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Aziz, Fitua M. "The Study of Antibacterial Activity of Juglans Regia and Thymus Vulgaris Seeds." Al Mustansiriyah Journal of Pharmaceutical Sciences 7, no. 1 (June 1, 2010): 64–72. http://dx.doi.org/10.32947/ajps.v7i1.318.
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It has been well known since ancient times that medicinal plant have antimicrobial activity because of the presence of substances, therefore they become important sources of drugs production. Thymus vulgaris and Juglans regia have active compounds make them have antibacterial properties.Antibacterial effects of different concentrations ranging from (3.1- 50mg/ml) of hexane and methanol extracts of Thymus vulgaris and Juglans regia seeds was determined by using agar well diffusion method on clinical strains: Staphylococcus aureus, Listeria monocytogenes, Streptococcus, pyogenes, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Proteus vulgaris, Salmonella typhi Klebsiella pneumoniae and Enterococcus sp.Methanol extract of Juglans regia seed was the most active followed by methanol extract of Thymus vulgsris seeds. The activity of Juglans regia extractdetermined by measuring inhibition zone as following:Staphylococcus aureus, Proteus vulgaris, Listeria monocytogenes and Streptococcus pyogenes showed inhibition zone at concentration of 3.1-50 mg/ml. Bacillus subtilis and Klebsiella pneumoniae showed inhibition zone of 12.5-50 mg/ml. Pseudomonas aeruginosa showed sensitivity at concentrations of 25-50mg/ml. There was no inhibition zone for Escherichia coli, Salmonella.typhi and Enterococcus sp.Methanol extract of Thymus vulgaris showed activity at concentrations of 6.25-50mg/ml for Listeria monocytogenes, and Bacillus subtilis.Escherichia coli, Staphylococcus. aureus, Proteus vulgaris, Klebsiella pneumoniae and Streptococcus pyogenes showed sensitivity at concentration of 12.5–50 mg/ml. Pseudomonas aeruginosa sensitive at concentration of 50- 25mg/ml. There was no inhibition zone for Salmonella.typhi and Enterococcus sp. Hexane extracts of both plant seeds were less active then methanol extract against tested bacteria.These results support the notion that plant extracts may have a role as pharmaceutical and preservatives.
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AN, Usman, J. Aliyu, B. Christiana, U. Umar, AI Adedayo, BJ David, and KF Adamu. "Isolation and characterization of aerial bacteria from a microbiology laboratory environment." Journal of Bio-Science 26 (December 26, 2019): 41–48. http://dx.doi.org/10.3329/jbs.v26i0.44663.
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Sterile nutrient agar (NA) plates were exposed to the inner and outer environment of the laboratory for 15 minutes before the commencement of laboratory work in the morning and after closure of activities in the evening after which the plates were incubated at 37oC for 24 hours. Antibiotic susceptibility of the bacterial isolates was done using agar well diffusion technique. The aerial bacterial load outside the laboratory was found to be higher than inside the laboratory. Bacteria isolated include members of the genus Staphylococcus, Bacillus, Lactobacillus, Salmonella, Micrococcus, Streptococcus, Proteus and Escherichia. Bacillus subtilis had the highest frequency of occurrence (31.0%) followed by alpha haemolytic streptococcus (19.0%) while the least frequency of occurrence was shown by Proteus bulgaricus and Proteus species (1.7%). All the bacteria were sensitive to antibiotics tested except Micrococcus luteus. They were equally found to be sensitive to the anti-microbial agent (antiseptic and disinfectant) tested except for the hand sanitizer to which most isolates displayed high resistance. The presence of a wide variety of pathogenic microorganisms in the laboratory shows that the environment requires regular cleaning and disinfection to enhance the safety of staff and students using the laboratory.
J. bio-sci. 26: 41-48, 2018
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Ryazanova, A. G., L. Yu Aksenova, A. A. Zuenco, T. M. Gridina, N. P. Buravtseva, and E. I. Eremenko. "Quality control of batches of bacteriological examination kits «Selective differential-diagnostic medium for recovery of Bacillus anthracis and closely related bacilli of Bacillus cereus group»." Bulletin of the Russian Military Medical Academy 20, no. 3 (December 15, 2018): 144–47. http://dx.doi.org/10.17816/brmma12316.
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Possibilities of quality control of experimental series of a set of reagents for bacteriological studies «Selective differential diagnostic environment for the isolation of the causative agent of anthrax and closely related bacilli of the Bacillus cereus group» is justified. Findings of the investigation of growth properties of the selective medium showed typical colonial morphology of virulent strains of Bacillus anthracis (B. anthracis 81/1, B. anthracis 1(CО), B. anthracis 1193, B. anthracis И-363, B. anthracis 1283) and its vaccine strains B. anthracis CTI-1, B. anthracis Ihtiman, B. anthracis Sterne 34 F2, B. anthracis 55, B. anthracis 71/12 - II Tsenkovsky’s vaccine). The colour of the batches of selective medium and Hottinger’s agar around the Bacillus anthracis colonies was unchanged. Whereas, the colour of selective medium around colonies of closely related saprophytes of genus Bacillus discoloured to bright-yellow, allowing to differentiate colonies of anthracic micro germs and closely related microorganisms. Presence of inhibiting properties of the selective medium was confirmed using heterologous germ cultures (Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Proteus vulgaris HX-19) which showed no growth in all series of the tested medium. Meanwhile, the typical colonial morphology of strains of these microorganisms was observed on Hottinger’s agar.
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Yılmaz, Meral, Turgay Tay, Merih Kıvanç, Hayrettin Türk, and Ayşen Özdemir Türk. "The Antimicrobial Activity of Extracts of the Lichen Hypogymnia tubulosa and Its 3-Hydroxyphysodic Acid Constituent." Zeitschrift für Naturforschung C 60, no. 1-2 (February 1, 2005): 35–38. http://dx.doi.org/10.1515/znc-2005-1-207.
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The antimicrobial activity and the MIC values of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen Hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent have been investigated against some microorganisms. At least one of the extracts or 3-hydroxyphysodic acid showed antimicrobial activity against Aeromonas hydrophila, Bacillus cereus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Listeria monocytogenes, Proteus vulgaris, Salmonella typhimurium, Staphylococcus aureus, Streptococcus faecalis, and Candida albicans. No antifungal activity of the extracts has been observed against ten filamentous fungi.
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Marliyana, Soerya Dewi, Didin Mujahidin, Yana M. Syah, and Yaya Rukayadi. "Time-Kill Assay of 4-Hydroxypanduratin A Isolated from Kaempferia Pandurata Against Foodborne Pathogens." Molekul 12, no. 2 (November 30, 2017): 166. http://dx.doi.org/10.20884/1.jm.2017.12.2.363.
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Time–kill assay was performed for 4-hydroxypanduratin A that was isolated from Kaempferia pandurata rizhome against four important foodborne pathogens, namely Bacillus cereus ATCC 21772, Bacillu subtilis ATCC 6633, Staphylococcus aureus ATCC 29737, and Proteus mirabilis ATCC 21100. The methods have been investigated in term of Minimum Inhibitory Concentration (MIC) and killing time curve using methods of Clinical and Laboratory Standards Institute (CLSI) guidelines. The results showed that 4-hydroxypanduratin A rapid acting in killing bacteria as follow: B. cereus : 1×MIC for 4 h, P. mirabilis: 4×MIC for 0.5 h, meanwhile B. subtilis and S. aureus were 1×MIC for 2 h. In conclusion, 4-hydroxypanduratin A showed strong antimicrobial activity against four important foodborne pathogens.
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Durojaye, Hammed Abiodun, and Georgia Chinemenwa Agu. "ANTIBIOTICS PRODUCING BACTERIA ISOLATED FROM FARMLANDS." Bacterial Empire 2, no. 4 (December 5, 2019): 99. http://dx.doi.org/10.36547/be.2019.2.4.99-102.
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The need for new antibiotics has been highlighted recently with the increasing pace of emergence of drug resistance pathogens. Emerging strains of bacteria resistant to most advanced antibiotics have become issues of very important public health concern. Modification of existing antibiotics with the addition of side chains or other chemical group and genomics based drug targeting have been the preferred method of drug development at the corporate level in recent years. In this regard, soil samples were collected from farmlands located in Ibadan in Oyo state, Ago – Iwoye, and Ikenne in Ogun state, Nigeria. Two putative Streptomyces strains and Bacillus strains isolated from the 16 selected farmland soils were characterized and assessed for antibiotic production and activity against a wide range of bacteria including Klebsiella pneumonia, Serratia marscens, Proteus vulgaris, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis. The extracts of the putative obtained from Streptomyces somaliensis, Streptomyces anulatus, Bacillus megaterium, and Bacillus subtilis showed activities against minimum of 3 and maximum of the 4 of the 7 tested bacteria. Inhibition zones were found to range between 2.0 - 25.0 mm diameters at a concentration of 1ml. The minimum inhibitory concentrations (MICs) of the crude extracts against the tested organisms ranged from 50% and above. Bacillus megaterium, and Streptomyces somaliensis were found to inhibit all the pathogenic bacteria, while S. anulatus was unable to inhibit Proteus vulgaris and Staphylococcus aureus, and B. Subtilis was unable to inhibit Enterococcus faecalis.
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Türk, Ayşen Özdemir, Meral Yılmaz, Merih Kıvanç, and Hayrettin Türk. "The Antimicrobial Activity of Extracts of the Lichen Cetraria aculeata and Its Protolichesterinic Acid Constituent." Zeitschrift für Naturforschung C 58, no. 11-12 (December 1, 2003): 850–54. http://dx.doi.org/10.1515/znc-2003-11-1219.
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Abstract In this study, the antimicrobial activity of the acetone, diethyl ether and ethanol extracts of the lichen Cetraria aculeata has been investigated. The extracts were tested against twelve bacteria and eight fungi and found active against Escherichia coli, Staphylococcus aureus, Aeromonas hydrophila, Proteus vulgaris, Streptococcus faecalis, Bacillus cereus, Bacillus subtilis, Pseudomonas aeruginosa, Listeria monocytogenes. No antimicrobial activity against the fungi was detected. It was determined that only one substance in the extracts has antimicrobial activity and it was characterized as protolichesterinic acid. The MICs of the extracts and protolichesterinic acid were also determined.
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Yılmaz, Meral, Ayşen Özdemir Türk, Turgay Tay, and Merih Kıvanç. "The Antimicrobial Activity of Extracts of the Lichen Cladonia foliacea and Its (–)-Usnic Acid, Atranorin, and Fumarprotocetraric Acid Constituents." Zeitschrift für Naturforschung C 59, no. 3-4 (April 1, 2004): 249–54. http://dx.doi.org/10.1515/znc-2004-3-423.
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The antimicrobial activity of the chloroform, diethyl ether, acetone, petroleum ether, and ethanol extracts of the lichen Cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents against 9 bacteria and fungi has been investigated. The extracts and pure compounds alone were found active against the same bacteria and the same yeasts. Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Streptococcus faecalis, Proteus vulgaris, Listeria monocytogenes, Aeromonas hydrophila, Candida albicans, and Candida glabrata growth were inhibited. In addition, the MICs of the extracts, (-)-usnic acid, atranorin and fumarprotocetraric acid were determined.
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Candan, Mehmet, Meral Yılmaz, Turgay Tay, Merih Kıvança, and Hayrettin Türk. "Antimicrobial Activity of Extracts of the Lichen Xanthoparmelia pokornyi and its Gyrophoric and Stenosporic Acid Constituents." Zeitschrift für Naturforschung C 61, no. 5-6 (June 1, 2006): 319–23. http://dx.doi.org/10.1515/znc-2006-5-603.
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The antimicrobial activity of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen Xanthoparmelia pokornyi and its gyrophoric acid and stenosporic acid constituents has been screened against some foodborne bacteria and fungi. Both the extracts and the acids showed antimicrobial activity against Aeromonas hydrophila, Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Proteus vulgaris, Staphylococcus aureus, Streptococcus faecalis, Yersinia enterocolitica, Candida albicans and Candida glabrata. The extracts were inactive against the tested filamentous fungi. The MIC values of the extracts and the acids for the bacteria have also been determined.
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Misto, Y. I. P., R. D. M. C. Sitorus, I. D. Permatasari, E. Noor, and T. C. Sunarti. "Formulation and Storage Stability of Nanoencapsulated Proteus penneri and Bacillus aerophilus." IOP Conference Series: Earth and Environmental Science 209 (December 19, 2018): 012040. http://dx.doi.org/10.1088/1755-1315/209/1/012040.
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Zanzarini, Taise Jordão, and Dora Inés Kozusny-Andreani. "Efeito do gás ozônio na descontaminação de canetas de bisturi elétrico." Revista Ibero-Americana de Ciências Ambientais 10, no. 4 (September 4, 2019): 112–20. http://dx.doi.org/10.6008/cbpc2179-6858.2019.004.0009.
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Uma das principais preocupações na prestação da assistência à saúde está relacionada ao processamento dos produtos utilizados. Portanto, essa qualidade no processamento dos produtos só será efetiva, quando reduzir ou destruir a carga microbiana, mantendo sua funcionalidade e integridade. Assim sendo, o gás ozônio, conhecido pela sua ação antimicrobiana, surge como potencial alternativa para facilitar alguns mecanismos de esterilização de alguns instrumentais médico-hospitalares. Objetivou-se, nesta pesquisa, estimar a carga microbiana e avaliar a eficácia do gás ozônio na descontaminação de canetas de bisturi elétrico nos diferentes tempos de tratamento. Foram coletadas amostras das canetas de bisturi elétrico antes do tratamento com ozônio e 5, 10 e 15 minutos após a exposição ao gás, e realizadas as culturas para bactérias e fungos em meios seletivos e não seletivos, sempre em triplicata. Os resultados foram avaliados por meio do teste de Kruskal-Wallis com posterior teste de comparação múltipla de Dunn. Os microrganismos isolados antes do tratamento foram: Staphylococcus aureus, Escherichia coli, Candida albicans, Proteus vulgaris, Micrococcus spp, Bacillus spp., Proteus mirabilis. Após a exposição ao gás ozônio, pôde-se observar que nos microorganismos Staphylococcus aureus, Micrococcus spp. e Bacillus spp., o ozônio promoveu a destruição dos microrganismos no tempo de 10 minutos. Na Escherichia coli, Proteus mirabilis e Proteus vulgaris essa morte aconteceu já na exposição a 5 minutos. O único microrganismo que necessitou de 15 minutos de exposição para ser eliminado totalmente foi a Candida albicans. Pode-se concluir que na metodologia adotada o gás ozônio foi eficiente na esterilização de canetas de bisturi elétrico, quando o tratamento se deu a 15 minutos de exposição.
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Garapati, Rajanikanth, and N. Ramesh. "IN VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY OF MICRO PROPAGATED CALLUS OF CURUULIGO ORCHIODES (BLACK MUSILLI) AGAR WELL DIFFUSION AND MINIMUM INHIBITORY CONCENTRATION (MIC)." International Journal of Current Pharmaceutical Research 9, no. 3 (May 5, 2017): 75. http://dx.doi.org/10.22159/ijcpr.2017.v9i3.19581.
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Objective: In vitro investigated the potential of methanol extracts of micro-propagated C. orchiodes in the antimicrobial property against the three gram-negative bacteria, two gram-positive and one fungal filament.Methods: The micro propagated callus methanol extract was examined against Escherichia coli, Proteus vulgaris, Salmonella typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Candida albicans. The zone of inhibitions are determined at 10 mg/ml concentration of methanol extracts of callus on agar well plate and MIC against tested microorganism.Results: The highest antibacterial activity recorded in Staphylococcus aureus Bacillus cereus and followed by Candida albicans. Antibacterial activity of leaf extracts of A. reticulata was also significant against the tested microorganisms Escherichia coli, Salmonella typhi, Proteus vulgaris, Pseudomonas aeruginosa compared to ciprofloxacin.Conclusion: Based on the above observations, these extracts were further evaluated for their effect on microorganisms causing infections like typhoid fever, urinary tract infections, septicemia, toxic shock syndrome, skin infection, nosocomial infection, arthritis and diarrhoea. The results also suggest that these plants serve a therapeutic purpose in the treatment bacterial infections.
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Shrivastava, Vikas. "PHYTOCHEMICAL PROFILING AND EFFICACY EVALUATION OF ANTIBACTERIAL POTENTIAL OF MYRISTICA FRAAGRANS SEED EXTRACTS AGAINST SELECTED MICROORGANISMS." Asian Journal of Pharmaceutical and Clinical Research 9, no. 6 (November 1, 2016): 241. http://dx.doi.org/10.22159/ajpcr.2016.v9i6.14287.
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Introduction: Traditional plants have been proved as novel source for health care remedies. Plants are the rich source of bioactive compounds which can be useful for health care purpose. Emergence of resistance among pathogenic bacteria against available antibiotics is a common phenomenon. So the plant based bioactive molecule may be an alternative for the development of new formulation. The use of natural bioactive molecules as parental compound for development of new formulation may be safer with less side effects as compared to synthetic chemical compounds. Myristica fraagrans seed are widely used in routine life from ancient time for health care. Traditionally it is being used in for stomach related complications. So the current study pertained to the phytochemical profiling of Myristica fraagrans seed and its efficacy evaluation against some clinically significant bacterial species.Methods: In this study ethanol and acetone extract of Myristica fraagrans seed (Jaiphal) were prepared via using soxhlet apparatus. Extract were qualitatively analysed to check the phytochemical composition. Then antibacterial efficacy of prepared extracts were analysed by agar well diffusion assay against Escherichia coli, Bacillus subtilis, Bacillus Magetarium, Proteus mirabilis.Results: The result of phytochemical profiling shows the presence of tannin, terpenoids, alkaloids, indole and phenol. Moreover Myristica fraagrans seed extract exhibiting significant antibacterial activity against selected microorganisms.Conclusion: Finding of the study concludes that Myristica fragrans seed ( jiphal ) possess the antimicrobial capabilities against infections and/or diseases caused by chosen microorganisms and seems to be useful in the discovery of novel antibiotic drugs. KEY WORDS: Myristica fraagrans (Jaiphal), Escherichia coli., Bacillus subtilis, Bacillus Magetarium, Proteus mirabilis., antibacterial activity etc.
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Carrasco-Acosta, Marina, Marta Santos-Garcia, and Pilar Garcia-Jimenez. "Marine Bacteria Associated with Colonization and Alteration of Plastic Polymers." Applied Sciences 12, no. 21 (November 1, 2022): 11093. http://dx.doi.org/10.3390/app122111093.
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The aim of this work was molecular identification of bacteria associated with marine sand at the drift line, where most plastic debris is deposited, and evaluation of the alteration of plastic polymers by them. Bacterial communities growing on plastic polymer surfaces may differentially cause surface alteration through exopolysaccharide production. This alteration can be analyzed by changes in spectra regions of colonized polymers compared to uncolonized polymers using Fourier Transform Infrared Spectroscopy (FTIR). In this study, bacteria located in sand at the drift line above sea water, where microplastics are most abundant, were isolated and identified through 16S rRNA. Six of the identified species produced exopolysaccharides, namely Bacillus thuringiensis, B. cereus, Bacillus sp. Proteus penneri, Alcaligenes faecalis and Myroides gitamensis. These bacteria species were inoculated into plates, each containing two frequently reported types of polymers at the drift line. Specifically, the two types of plastic polymers used were polypropylene and polystyrene spheres in whole and mechanically crushed states. Differences in bacterial growth were reported as inferred from weight increase of polypropylene and polystyrene spheres after 1-year long culture. Results also showed that Alcaligenes faecalis, Bacillus cereus and Proteus penneri colonized polypropylene spheres and modified spectra regions of FTIR. It is concluded that bacteria located in sand can be considered plastic-altering bacteria as changes in FTIR-spectra of polymers can be related to bioalteration.
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Liu, Yang, N. Ramesh Kumar, Qiliang Lai, Juan Du, Anatoly P. Dobritsa, Mansour Samadpour, and Zongze Shao. "Identification of strains Bacillus aerophilus MTCC 7304T as Bacillus altitudinis and Bacillus stratosphericus MTCC 7305T as a Proteus sp. and the status of the species Bacillus aerius Shivaji et al. 2006. Request for an Opinion." International Journal of Systematic and Evolutionary Microbiology 65, Pt_9 (September 1, 2015): 3228–31. http://dx.doi.org/10.1099/ijsem.0.000408.
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On the basis of 16S rRNA, rpoB, gyrB and pycA gene sequence analyses, characterization of biochemical features and other phenotypic traits and pulsed-field gel electrophoresis (PFGE) fingerprinting, it was ascertained that strains Bacillus aerius MTCC 7303T, Bacillus aerophilus MTCC 7304T and Bacillus stratosphericus MTCC 7305T do not conform to the descriptions of the type strains of the respective species. Strains MTCC 7303T and MTCC 7304T were indistinguishable from Bacillus altitudinis DSM 21631T, while strain MTCC 7305T should be classified as a representative of a Proteus sp. Our attempts to find other deposits of the type strains of these species were unsuccessful. Therefore, the results support the Request for an Opinion on the status of the species Bacillus aerophilus and Bacillus stratosphericus by Branquinho et al. [Branquinho, R., Klein, G., Kämpfer, P. & Peixe, L. V. (2015). Int J Syst Evol Microbiol 65, 1101]. It is also proposed that the Judicial Commission should place the name Bacillus aerius on the list of rejected names if a suitable replacement type strain cannot be found or a neotype is not proposed within two years following the publication of this Request (Rule 18c).
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Cansaran, Demet, Demet Çetin, M. Gökhan Halıcı, and Orhan Atakol. "Determination of Usnic Acid in Some Rhizoplaca Species from Middle Anatolia and their Antimicrobial Activities." Zeitschrift für Naturforschung C 61, no. 1-2 (February 1, 2006): 47–51. http://dx.doi.org/10.1515/znc-2006-1-209.
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Abstract Three species of lichens, Rhizoplaca chrysoleuca (Sm.) Zopf, Rhizoplaca melanopthalma (DC.) Leuckert & Poelt and Rhizoplaca peltata Ramonds Leuckert, were collected from middle Anatolia, Erciyes Mountain (Kayseri) in Turkey. Their usnic acid amounts were determined by HPLC in acetone extracts. In addition, antimicrobial activities of these extracts were determined against Escherichia coli (ATCC 35218), Enterococcus faecalis (RSKK 508), Proteus mirabilis (Pasteur Ens. 235), Staphylococcus aureus, Bacillus subtilis, Bacillus megaterium and Pseudomonas aeruginosa. It was shown that, as the usnic acid amount increased, the antimicrobial activities increased too. The usnic acid contents of Rhizoplaca species varied between 0.19-4.0% dry weight
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Pagar, Balasaheb P. "Synthesis and Antimicrobial Activity of Novel Oxothiazolidine Derivatives." Asian Journal of Organic & Medicinal Chemistry 5, no. 2 (2020): 91–96. http://dx.doi.org/10.14233/ajomc.2020.ajomc-p248.
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In this article, acid hydrazide 2, a functional group, was synthesized by the reaction of (4-chloro-12- methyl-16,17-dihydro-15-thia-6,11-diaza-cyclopenta[a]phenanthren-7-ylsulfanyl)acetic acid ethyl ester (1) with hydrazine yield (4-chloro-12-methyl-16,17-dihydro-15-thia-6,11-diazacyclopenta[a]- phenanthren-7-ylsulfanyl)acetic acid hydrazide (2) is discussed. The reactive acid hydrazide compound 2 was utilized for the synthesis of amides 3, Schiff’s bases 4 and thiazolidine 5 derivatives. The structures of target compounds were confirmed by elemental analysis and spectral data. The antimicrobial activity of new compounds were studied against Streptococcus sp., Bacillus megaterium, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Bacillus subtilis, Proteus valgaris and Pseudomonas aeroginosa by the agar well diffusion method. Compounds 4b, 5a, 5b and 5c showed good antimicrobial activity
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Kravchenko, N. "Microbial Regulation of the Antagonistic Activity of Lactic Acid Bacteria Strains under the Influence of Transient Bacilli of the Bacillus Genus." Agricultural Science and Practice 2, no. 2 (July 15, 2015): 45–49. http://dx.doi.org/10.15407/agrisp2.02.045.
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Aim. To study the effect of metabolites and components of aerobic spore-forming bacilli cells (MCASB) of the Bacillus genus on the antagonistic activity of lactic acid bacteria. Methods. Lactic acid bacteria, sampled from rectal and oral swabs from calves, were used in the study. Strains of conditionally pathogenic Escherichia coli, 055k59 No. 3912, Staphylococcus aureus No. 906, Proteus mirabilis, No. 3177, obtained from the collection of the city sanitary-epidemiological inspection service, were used as test cultures. MCASB were prepared using autoclaved microbial mass of Bacillus subtilis production strain 44-p via treatment with lysozyme and ultra- sound. The antagonistic activity of lactic acid bacteria was determined by agar blocks method. Results. It was demonstrated that the addition of MCASB to the culture medium stimulates the antagonistic activity of lactic acid bacteria to pathogenic and conditionally pathogenic microorganisms of intestinal bacteria. The highest average stimulation index (2.56) of the antagonistic activity of lactic acid bacteria was found for E. coli 055k59 No. 3912. Conclusions. The results obtained may be used to improve the microbiological foundations of elaborating novel probiotic preparations.
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Maina N, Nseabasi, E. G. Vinkings, I. U. Bassey, A. A. Unimke, and L. O. Abulawor. "Screening the Milieu of an abattoir for Bacteria of Public health importance in Makurdi, Benue State, Nigeria." Journal of Microbiology and Biotechnology Research 7, no. 2 (May 14, 2017): 1. http://dx.doi.org/10.24896/jmbr.2017721.
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The study investigated the bacteriological content in a major abattoir located approximately 300 meters from a densely populated and cultivated area along the Benue River. Enumeration of bacterial cells from samples in the study yielded relatively high mean count of 9.4 x 105 and 7.3 x 105 from effluent and soil samples respectively. Bacteria isolated from both samples included; Escherichia coli, Streptococcus sp, Salmonella sp, Pseudomonas sp, Shigellasp, Enterobacter sp, Staphylococcus sp, Bacillus sp, Brucella sp, Proteus sp, Micrococcus sp etc. Escherichia coli recorded an occurrence of 18.53% in effluents and 16.16% in soil while Proteus species and Brucella sp had an occurrence of 9.59% and 1.39% respectively in soil samples. Antibiotic sensitivity screening using seveenteen (17) antibiotics disc (Optun Nig.) viz: Tarivid (10 µg), peflacine (10 µg), Agumentin (30 µg), Gentamycin (10 µg), Streptomycin (30 µg), Ceporex (10 µg), Nalidixic acid (30 µg), Ciprofloxacin (10 µg), Norfloxacin (10 µg), Rifampicin (µg), Erythromycin (µg), Chloramphenicol (µg), Ampiclox (30 µg), Levofloxacin (10µg). A marked level of resistance was observed among the isolates. However, Escherichia coli indicated sensitivity to peflacine, Shigellasp indicated sensitivity to augmentin, Enterococcus and Bacillus species indicated sensitivity to ciprofloxacin and streptomycin respectively.
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Cansaran, Demet, Didem Kahya, Ender Yurdakulol, and Orhan Atakol. "Identification and Quantitation of Usnic Acid from the Lichen Usnea Species of Anatolia and Antimicrobial Activity." Zeitschrift für Naturforschung C 61, no. 11-12 (December 1, 2006): 773–76. http://dx.doi.org/10.1515/znc-2006-11-1202.
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Abstract Six species of lichens, such as Usnea florida, Usnea barbata, Usnea longissima, Usnea rigida, Usnea hirta and Usnea subflorida, were collected from different areas of Anatolia (district of Antalya, Karabük, Çankırı, Giresun and Trabzon) in Turkey. Their usnic acid amounts in acetone extracts were determined by HPLC. In addition, antimicrobial activities of these extracts were determined against Escherichia coli (ATCC 35218), Enterococcus faecalis (RSKK 508), Proteus mirabilis (Pasteur Ens. 235), Staphylococcus aureus, Bacillus subtilis and Bacillus megaterium. It was shown that with increasing amount of usnic acid, the antimicrobial activity increased. Usnic acid contents of Usnea species varied between 0.22-6.49% of dry weight.
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Murthy, Y. L. N., Padma Suhasini, and Jha Anjali. "Synthesis and characterization of new 2-amino-4-(substituted phenyl)-6-(7’’-methoxy, 3’’,4’’-dihydro, 2’’,2’’-dimethyl-2hbenzopyran) pyrimidines and their bio evaluation." Journal of the Serbian Chemical Society 77, no. 7 (2012): 859–66. http://dx.doi.org/10.2298/jsc110923008m.
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A series of eight novel 2-amino-4-(substituted phenyl)-6-(7"- methoxy, 3",4"-dihydro, 2",2"-dimethyl-2H-benzopyran) pyrimidines were designed and synthesized by utilizing benzene-1,3-diol as starting material. The structures of the isolated products (6a-h) were established through 1HNMR, 13C-NMR, and FT-IR spectroscopic techniques and elemental analysis. The synthesized compounds were utilized for screening antimicrobial properties against gram positive (Bacillus pumilus, Bacillus subtilis, Streptococcus faecalis), gram negative bacteria (Enterococcus faecalis, Escheria coli, Proteus vulgaris) and fungi (Pencillian expasom, Aspergillus niger, Candida albicans) using Ampicillin and Ketoconazole. Most of the compounds showed moderate to high antibacterial and antifungal activities against studied strains with inhibition zones between 8 and 30 mm.
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Sun, Chunxiao, Ziping Zhang, Zilin Ren, Liu Yu, Huan Zhou, Yaxin Han, Mudassir Shah, et al. "Antibacterial Cyclic Tripeptides from Antarctica-Sponge-Derived Fungus Aspergillus insulicola HDN151418." Marine Drugs 18, no. 11 (October 26, 2020): 532. http://dx.doi.org/10.3390/md18110532.
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Three new aspochracin-type cyclic tripeptides, sclerotiotides M–O (1–3), together with three known analogues, sclerotiotide L (4), sclerotiotide F (5), and sclerotiotide B (6), were obtained from the ethyl acetate extract of the fungus Aspergillus insulicola HDN151418, which was isolated from an unidentified Antarctica sponge. Spectroscopic and chemical approaches were used to elucidate their structures. The absolute configuration of the side chain in compound 4 was elucidated for the first time. Compounds 1 and 2 showed broad antimicrobial activity against a panel of pathogenic strains, including Bacillus cereus, Proteus species, Mycobacterium phlei, Bacillus subtilis, Vibrio parahemolyticus, Edwardsiella tarda, MRCNS, and MRSA, with MIC values ranging from 1.56 to 25.0 µM.
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Klaus, Anita, Damir Beatovic, Miomir Niksic, Slavica Jelacic, Viktor Nedovic, and Tanja Petrovic. "Influence of ethereal oils extracted from Lamiaceae family plants on some pathogen microorganisms." Zbornik Matice srpske za prirodne nauke, no. 115 (2008): 65–74. http://dx.doi.org/10.2298/zmspn0815065k.
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As pathogen microorganisms can be found in different kinds of food, using of natural antimicrobial compounds, like ethereal oils, could be important in the preservation of different groceries. To evaluate antimicrobial activity of ethereal oils extracted from Lamiaceae family plants - Rosmarinus officinalis L., Thymus vulgaris L., Majorana hortensis M o e n c h, and Salvia officinalis L screening of their effects against food borne bacteria Staphylococcus aureus, Enterococcus faecalis, Proteus mirabilis, Salmonella enteritidis, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Escherichia coli, Escherichia coli O157:H7, Listeria monocytogenes and yeasts Candida albicans and Saccharomyces cerevisiae were applied. All investigated concentrations and pure Majorana hortensis and Thymus vulgaris ethereal oils showed microbicidal effect on majority of tested microorganisms.
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Enaigbe, Andrew A., Obhioze Augustine Akpoka, and Stanley Odaro Imade. "ASSESSMENT OF 16S rRNA SEQUENCE AND HETEROTROPHIC PLATE COUNT (HPC) METHODS OF IDENTIFYING BACTERIA FROM DRINKING WATER SYSTEMS IN BENIN CITY METROPOLIS." Bacterial Empire 2, no. 3 (October 4, 2019): 63. http://dx.doi.org/10.36547/be.2019.2.3.63-69.
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A variety of simple culture-based tests which are proposed to recover a wide range of microorganisms from water are collectively known as heterotrophic plate count (HPC) and used as an indirect indicator to give information about water quality. The aim of this study was to assess the heterotrophic plate count (HPC) culture-based dependent and 16S rRNA independent techniques of identifying bacteria. The HPC was conducted by incubating a filtered sample of water on R2A agar plates and enumerating the number of resultant bacterial colonies that grow on each plate. The molecular analysis was performed by extracting the deoxyribonucleic acid (DNA) from bacterial isolate and polymerase chain reaction (PCR) was done to obtain the amplicons (PCR products). Purified PCR products were sequenced by ABI V3.1 Big dye kit and the analysis of sequence was conducted by the basic local alignment search tool (BLAST) to identify their closest relatives. A total number of 17 isolates of Pseudomonas, Bacillus and Proteus were phenotypically identified, while the nucleotide sequence revealed the presence of 59 diverse strains with distribution (percentage) of Pseudomonas (25) 42.2 %, Bacillus 17 (28.8 %) and Proteus (17) 28.8 %. The investigated isolates when compared from gene data-base recorded genetic relatedness with similarity index of 61 to 100 %. The 16S rRNA gene sequences from DNA extractions showed microbial consortia in drinking water to comprise of a broad array of bacterial diversity, including those of critical concern to human health such as Pseudomonas and Bacillus. Finally, there was no significant correlation between the HPC test and 16S rRNA sequence analysis.
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Narakornwit, Worrakanya, and Juree Charoenteeraboon. "Determination of Antimicrobial Activity from Various Plant Parts of <i>Typha angustifolia</i> Using Agar Disc Diffusion and Bioautography." Key Engineering Materials 914 (March 21, 2022): 105–10. http://dx.doi.org/10.4028/p-6q5lz7.
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Fruit, leaf, fruit stalk, and rhizome of Typha angustifolia, which is abundantly available in Thailand, were investigated for antimicrobial activities. The results showed that fruit ethanolic extract had high activity against Staphylococcus aureus and Bacillus subtilis similar to the fruit stalk and rhizome extracts whereas the lowest antibacterial activities was found in the leaf extract. Only rhizome extract had antifungal activity against Candida albicans. The fruit extract was chosen for furthur study because of its antibacterial action, ease of collecting, and preparation before usage. This fruit ethanolic extract was semi-purified by partition with petroleum ether, chloroform and ethylacetate, respectively and subsequently tested for antimicrobial activities by disc diffusion against S. aureus, Staphylococcus epidermidis, Sarcina lutea, Streptococcus faecalis, Streptococcus pneumoniae, Micrococcus flavus, Bacillus cereus, Bacillus pumillus, B. subtilis, Shigella somnei, Proteus mirabilis, Proteus valgalis, Alcaligenes fecalis, Serratia marcescens, Salmonella paratyphi A, Salmonella typhi, Escherechia coli, Pseudomonas aeruginosa, and Saccharomyces cerevisiae. The inhibitory zone against gram-positive bacteria was broader in all partitioned fractions than in gram-negative bacteria and yeast. Furthermore, none of the fractions were able to prevent the development of S. marcescens, P. aeruginosa, or E. coli. The inhibition zone of the ethyl acetate fraction was larger than that of chloroform and petroleum ether. In comparison to the other fractions, the chloroform fraction demonstrated the broadest inhibition zone against B. subtilis. Based on the bioautography investigation, it was reasonable to conclude that there were at least five antibacterial chemicals against B. subtilis. Therefore, fruit of T. angustifolia has the potential for use in the discovery of new antibacterial agents.
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Hasan, R., M. N. H. Rony, M. S. A. Sarker, M. Z. Tareq, and R. Ahmed. "Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh." Journal of Bioscience and Agriculture Research 26, no. 02 (December 30, 2020): 2211–16. http://dx.doi.org/10.18801/jbar.260220.270.
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Bacteria are essential elements of natural environments. As bacteria are the key critical components of food webs and nutrient cycles, they contribute to ecosystem functioning via mutualists and pathogens for larger species. The present study has provided substantial grounds to confirm that microbial communities present in natural environments are much more diverse. Here, we tend to study a singular environment Lawachara National Park. Total 125 bacterial strains were isolated using serial dilution method. Thirteen unique colonies were selected, cultured and characterized by gram staining and biochemical tests. Based on morphological, biochemical, 16S rDNA gene sequencing and phylogeny analysis revealed that the isolates were identified as Staphylococcus aureus, Micrococcus luteus, Bacillus cereus, Bacillus subtilis, Bacillus thuringensis, Serratia marcescens, Aeromonas hydrophila, Enterobacter cowanii, Acidobacterium capsulatum, Klebsiella pneumonia, Pseudomonas aeruginosa, Escherichia coli and Proteus mirabilis. This study serves as a baseline survey of bacterial diversity in the Lawachara National Park.
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Mamangkey, Jendri, Dwi Suryanto, Erman Munir, and Apon Zaenal Mustopa. "Antibacterial and Antioxidant Activity of Newly Keratinolytic Bacteria, Azotobacter chroococcum B4." International Journal of PharmTech Research 13, no. 2 (2020): 123–27. http://dx.doi.org/10.20902/ijptr.2019.130215.
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Keratinolyticbacteria of A. chrocoocum B4 was evaluated for its potential of antibacterial and antioxidant activity. Kirby-Bauer method was used to know antibacterial potential of B4 againtsStaphylococcus aureus, S. epidermidis, Bacillus cereus, Bacillus pumilus, Bacillus subtilis, Listeria monocytogenes, Proteus sp., enteropathogenicEscherichia coli, Enterobactersakazakii, and Salmonella enterica. Antioxydant test was done using DPPH radical scavenging activity assay with ascorbic acid as a controll. In this study, hydrolysate of pellet, dialysis, and fraction 25 of B4 keratinase purification of previous study was used for antibacterial and antioxidant test. The result showed that B4 hydrolysatesinhibited Gram positive pathogenic bacteria such as Staphylococcus aureus and Listeria monocytogenes, and Gram negative Enterobacter sakazakii. All hydrolysates showed to have antioxidant properties in which fraction 25 showed higher compared to that of others. This study showed poultry waste-derived keratinase of B4 might be useful as supplementary protein, antibacterial, and antioxidant in the animal feed formulations.
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Candan, Mehmet, Meral Yılmaz, Turgay Tay, Murat Erdem, and Ayşen Özdemir Türk. "Antimicrobial Activity of Extracts of the Lichen Parmelia sulcata and its Salazinic Acid Constituent." Zeitschrift für Naturforschung C 62, no. 7-8 (August 1, 2007): 619–21. http://dx.doi.org/10.1515/znc-2007-7-827.
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The antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen Parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. All of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against Aeromonas hydrophila, Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Proteus vulgaris, Yersinia enterocolitica, Staphylococcus aureus, Streptococcus faecalis, Candida albicans, Candida glabrata, Aspergillus niger, Aspergillus fumigatus, and Penicillium notatum. Salazinic acid did not show antimicrobial activity against L. monocytogenes, P. vulgaris, Y. enterocolitica, and S. faecalis but showed activity against Pseudomonas aeruginosa and Salmonella typhimurium as well. The MIC values of the extracts and the acid for the bacteria and fungi have also been determined
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Merghache, D., Z. Boucherit-Otmani, I. A. El Haci, I. Chikhi, and K. Boucherit. "Inhibitory Effect of Quercus ilex Wood Ash on the Growth of Pathogenic Microorganisms." Phytothérapie 16, S1 (December 2018): S269—S272. http://dx.doi.org/10.3166/phyto-2018-0071.
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This study was conducted to determine the antimicrobial (antibacterial and antifungal) activity of green oak (Quercus ilex) wood ash from Beni Snous region (Tlemcen, Algeria). For the evaluation of antimicrobial potency, we tested the effect of different ash concentrations on reference bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter cloacae, and Proteus mirabilis) and reference yeasts (Candida albicans ATCC 10231 and Candida albicans 444IP). The antibacterial activity test results show that Gram-positive bacteria have a higher sensitivity than Gram-negative bacteria. Concerning the antifungal activity, inhibition of yeast growth was found even at low ash concentrations (5%). The results of this preliminary investigation thus revealed that wood ash from green oak exerts a remarkable antimicrobial activity.
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Oputah, Samuel L., Kolawole O. Ajanaku, Raphael C. Mordi, Joseph A. O. Olugbuyiro, Shade J. Olorunshola, and Dominic E. Azuh. "Phytochemical and Antibacterial Properties of Ethanolic Seed Extracts of Chrysophyllum albidum (African Star Apple)." Oriental Journal of Physical Sciences 1, no. 1-2 (December 26, 2016): 05–09. http://dx.doi.org/10.13005/ojps01.0102.02.
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Phytochemical and antibacterial properties of ethanolic extract of the seeds of African Star Apple (Chrysophyllum albidum) were investigated. The phytochemical result revealed the presence of saponins, carbohydrates, flavonoids, quinones, cardiac glycosides, fatty acids and terpenoids. The antibacterial activity was studied using agar well diffusion method at different concentrations against six pathogenic bacterial strains, three Gram-positive (Staphylococcus aureus, Micrococcus varians and Bacillus cereus) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris). Significant inhibitory activities were exhibited by the ethanolic seed extracts for all test organisms except Bacillus cereus. Zone of inhibition of the crude ethanolic extract was correlated with that of a standard antibiotic Gentamicin, for antibacterial activity. The results indicated a notable inhibition of the bacterial growth.