Academic literature on the topic 'Bacillus proteus'
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Journal articles on the topic "Bacillus proteus"
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JP, Omorodion Nnenna, and Wisdom John. "Effects of Wrapping Materials on the Microbial Population of Steam Bean Pudding (Moimoi)." Journal of Food Technology Research 9, no. 1 (April 7, 2022): 80–93. http://dx.doi.org/10.18488/jftr.v9i1.2955.
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The effects of wrapping materials on the microbial population of Moimoi were assessed. A total of thirty samples of wrapped Moimoi of plastic, waterproof and aluminum foil was collected from food vending sites located in Choba. The samples were taken to the laboratory and analyzed using standard microbiological methods. The total prevalence of bacteria that occurred from foil Moimoi were Micrococus (35%), Bacillus (25%) , Staphylococcus (20%) and Proteus (20%). The total prevalence of bacteria obtained on plastic Moimoi were Staphylococcus (27%), Bacillus subtilis (23%) Proteus sp (23%), Micrococcus sp (13%) and Bacillus cereus (14%). Total prevalence obtained from nylon Moimoi were Staphylococcus (27%), Bacillus cereus (23%), Proteus (18%), Micrococcus (18%) and Bacillus subtilis (14%). The mean total bacteria count, Staphylococcus count and coliform count obtained ranged from 9.8 x103 - 1.35 x103 cfu/g. The result of the study further shows that most of the gram-positive isolates such as Staphylococcus sp, Micrococcus sp, Bacillus subtilis and Bacillus cereus were resistant to antibiotics of ceftriaxone, cloxacillin, Augmentin, ceftazidime and cefuroxime while the gram-negative bacteria isolated Proteus sp was resistance to Augmentin and cefuroxime but susceptible to gentamycin, erythromycin and cefuroxime. Contamination of these wrapped Moimoi samples is due to poor personal hygiene and sanitation among food handlers. To prevent outbreak of food poisoning, users of this wrapping materials should wash wrapping materials with clean water before using and food in these wrapping materials should be consumed immediately to reduce multiplication of microorganisms.
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Ainul, Asri, M. Hasbi, and Eko Purwanto. "Isolation and Identification of Biosurfactant Producing Bacteria From Workshop Wastewater." Ilmu Perairan (Aquatic Science) 9, no. 1 (March 30, 2021): 31. http://dx.doi.org/10.31258/jipas.9.1.p.31-37.
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Automotive workshop activities produce oil ills that may pollute waters around the workshop area. The oil-polluted water may inhabit biosurfactant producing bacteria that are able to degrade the oil. A study aimed to isolate and identify the bacteria has been conducted from July to September 2020. The bacteria samples were sampled from workshop wastewater at Kubang Raya street KM 2,5 Pekanbaru, Riau Province and sampling were conducted three times. The bacteria were isolated using TSB (Tryptone Soy Broth) and TSA (Tryptone Soy Agar) media and were identified by using biochemical methods. Results showed that there were seven types of bacteria, namely Providencia, Proteus, Acinetobacter, Bacillus, Aeromonas, Proteus and Serratia. The Emulsification index of Providencia was 38.8%, Proteus 50%, Acinetobacter 48.8%, Bacillus 52,1%, Aeromonas 47,6%, Proteus 54,7% and Serratia 48,8%. Data obtained from this study showed that all of the identified bacteria are able to produce biosurfactants.
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AGBABIAKA, Toyin Olayemi, and Blessing Olubunmi OLOFINTOYE. "Microbial Diversity in Water and Biofilm Samples from Well Sources in Ilorin Metropolis, Nigeria." Notulae Scientia Biologicae 11, no. 1 (March 21, 2019): 56–62. http://dx.doi.org/10.15835/nsb11110337.
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The aim of the study was to investigate the microbial populations of water and biofilm samples from seven hand dug wells in Ilorin, Nigeria, which are for private and public use. Physicochemical parameters such as pH, turbidity, total dissolved solids, temperature, conductivity, dissolved oxygen and biological oxygen demand were determined. Biofilm growths were induced from water samples obtained from selected wells. Twenty bacteria isolated were identified using morphological and biochemical characteristics as well as molecular methods and includes Shigella sonnei, Shigella dysenteriae, Micrococcus luteus, Bacillus sphaericus, Salmonella enteritidis, Proteus mirabilis strain IK-MB4-518F, Bacillus licheniformis strain RH104, Bacillus subtilis, Erwinia sp., Proteus vulgaris, Yersina sp., Bacillus cereus, Staphylococcus aureus, Serratia marcescens, Pseudomonas aeruginosa strain GS1, Pseudomonas aeruginosa strain 218B, Staphylococcus epidermidis, Vibrio sp., Escherichia coli and Bacillus pasteurii. Total bacterial count ranged from 1.8×104-1.53×106 cfu/ml. Water samples from these wells are therefore not potable and some of the isolates are potential health hazards to human population.
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Yusuf UA, Ekeleme IK, Owuna EJ, Ochai SS, and Obiekezie SO. "Effects of spent hydrocarbon on bacteria population." International Journal of Scholarly Research in Science and Technology 2, no. 1 (January 30, 2023): 001–9. http://dx.doi.org/10.56781/ijsrst.2023.2.1.0033.
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This study aimed at the effect of spent hydrocarbon contamination on microbial population in soil. Standard microbiological methods were used to determine the total heterotrophic count hydrocarbon utilization, isolation and identification of bacterial and effect of pH and spent hydrocarbon concentration on bacterial. The total heterotrophic bacterial count (THB) ranges from 6.2 ± 0.13 x106 to 3.2± 0.10 x106 cfu/g. The total hydrocarbon utilizing bacterial count ranges from 3.2 ± 0.13 x106 to 1.2± 0.10 x106 cfu/g. The bacterial isolated were Bacillus subtilis, Pseudomonas flourescens, Klebsiella aerogenes and Proteus hauseri. It was observed that Bacillus subtiliis had the highest occurrence from location E (50.0%). Pseudomonas flourescens from location A and B (33.3%) and Proteus hauseri had the highest occurrence from location C (66.6%). The effect of pH on bacterial growth rate analyzed showed that Bacillus subtilis had the highest turbidity at pH 6.5 (0.511 ± 0.15 nmm), Klebsiella aerogenes had the highest turbidity at pH 7.5 (0.233 ± 0.33nm), Pseudomonas flourescens was at pH 6.5 (0.723 ± 0.61 nm) and Proteus sp recorded highest turbidity at pH 6.5 (0.373 ± 0.22nm) followed by pH 5.5 (0.237 ± 0.19 nm). The effect of spent hydrocarbon concentration showed that Bacillus subtilis recorded highest turbidity at 10% concentration (0.744 ± 0.03 nm), Klebsiella aerogenes recorded highest at 10% concentration (0.321 ± 0.21 nm), Pseudomonas flourescens was at 10% concentration (0.887 ± 0.23 nm) and Proteus sp recorded highest turbidity at 10% concentration (0.378 ± 0.13 nm). From this study it was observed that indigenous bacterial had the ability to utilized the spent hydrocarbon if the pH of the soil is regulated.
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Makut, Makwin Danladi, Toyosi Michelle Adebayo, and Jibril Egwu Owuna. "Bioremediation of soil polluted with spent lubricating oil using bacteria isolated from soil in Abuja Metropolis." BIOMED Natural and Applied Science 02, no. 03 (December 10, 2022): 33–40. http://dx.doi.org/10.53858/bnas02033340.
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Background: The presence of spent hydrocarbon in soil is a serious problem to the environment hence study on bioremediation of soil polluted with auto-mechanic oil in Abuja Metropolis was carried out. Methods: A total of twenty (20) soil samples were collected, bacteria were isolated from the contaminated soil and identified using standard microbiological methods. The spent hydrocarbon utilization was determined using Atomic Adsorption UV Spectrometer. Results: The total viable count of the bacteria was 1.07 x 106 from Apo Mechanic village, 1.10 x 106 from Utako Mechanic workshops, 0.40 x 106 from Gwarinpa Mechanic workshops and 2.04 x 106 from Area one Mechanic workshops. The percentage occurrence of bacteria from Apo Mechanic village was Enterobacter species 40.0%, Pseudomonas synxantha 60.0%, Bacillus zanthoxyli 40.0% and Proteus vulgaris 20.0%. Utako Mechanic workshops were Enterobacter kobei 20.0%, Pseudomonas synxantha 40.0%, Bacillus zanthoxyli 20.0% and Proteus vulgaris 40.0%. Gwarinpa Mechanic workshops were Pseudomonas synxantha 20.0% and Bacillus zanthoxyli 20.0%. Area one Mechanic workshops were Enterobacter kobei 40.0% and Pseudomonas synxantha 40.0%. The effect of days on utilization of spent hydrocarbon showed that Pseudomonas synxantha had highest utilized of spent hydrocarbon 19.55mg/ml after 21 days. The effect of pH on utilization of spent hydrocarbon show that at pH 7.5, Enterobacter kobei, Bacillus zanthoxyli and Proteus vulgaris species had the highest utilization of spent hydrocarbon ranging from 5 9.33mg/ml-12.70mg/ml. Effect of temperature on utilization of spent hydrocarbon showed that at 28OC Enterobacter kobei, Pseudomonas synxantha, Bacillus zanthxyli and Proteus vulgaris had the highest utilization of spent hydrocarbon ranging from 5.51mg/ml- 11.11mg/ml. the bacteria isolated from the contaminated soil have the ability to utilized the hydrocarbon if the soil is amended with some mineral element as shown in this study. Conclusion: In conclusion bacteria isolates effectively bioremediated the automechanic oil polluted soil with a reduction of hydrocarbon pollutants.
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Patel, Binal R., MT Panchal, AJ Dhami, RA Mathakiya, and BB Bhanderi. "Bacterial Isolates from the Genital Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Buffaloes." INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY 15, no. 02 (November 25, 2019): 56–59. http://dx.doi.org/10.21887/ijvsbt.15.2.15.
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The study was carried out on 50 vaginal secretions/aspirates/discharge samples collected aseptically using syringe and pipette method from infertile (anestrus; endometritic, n = 6 each) buffaloes of villages nearby Anand and healthy cyclic (n = 5; proestus, estrus, metestrus, diestrus) as well as 3, 6 and 9 month pregnant (n = 6 each) buffaloes of University farm to identify the vaginal microorganisms based on routine cultural examination. In all 117 bacterial isolates were recovered from all 50 vaginal samples (100 %) of 35 buffaloes during different physio-pathological status. The bacteria isolated from vaginal mucus/aspirates of buffaloes during the follicular phase comprised Corynebacterium spp. as the most predominant isolate (28.57%) followed by E. coli, Bacillus Spp., Staphylococcus spp., Streptococcus spp., Salmonella spp., Proteus spp., and vaginal yeast, whereas during the luteal phase, the most predominant bacteria were E. coli (23.33%) followed by Corynebacterium spp., Bacillus spp., Staphylococcus spp., Streptococcus spp., and Klebsiella spp. In acyclic buffaloes, the most predominant bacteria isolated were Corynebacterium spp. (21.43%) Bacillus spp., Micrococcus spp., Pseudomonas, Staphylococcus spp., Streptococcus spp., E. coli and Salmonella spp., whereas the endometritic buffaloes evinced the most predominant bacterial isolates as Corynebacterium spp. and E. coli (20.00% each) followed by Bacillus spp., Salmonella, Proteus spp., Staphylococcus spp., Streptococcus spp., and Klebsiella spp. The major bacteria isolated during the entire period of pregnancy were E. coli, Micrococcus, Corynbacterium Spp., Bacillus spp., Staphylococcus spp. and Proteus. This study concludes rich bacterial diversity in the vagina of buffaloes during different physio-pathological status.
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Abdul-Jabbar, Amna M., Nehia N. Hussian, Hamdoon A. Mohammed, Ahmed Aljarbou, Naseem Akhtar, and Riaz A. Khan. "Combined Anti-Bacterial Actions of Lincomycin and Freshly Prepared Silver Nanoparticles: Overcoming the Resistance to Antibiotics and Enhancement of the Bioactivity." Antibiotics 11, no. 12 (December 10, 2022): 1791. http://dx.doi.org/10.3390/antibiotics11121791.
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Bacterial drug resistance to antibiotics is growing globally at unprecedented levels, and strategies to overcome treatment deficiencies are continuously developing. In our approach, we utilized metal nanoparticles, silver nanoparticles (AgNPs), known for their wide spread and significant anti-bacterial actions, and the high-dose regimen of lincosamide antibiotic, lincomycin, to demonstrate the efficacy of the combined delivery concept in combating the bacterial resistance. The anti-bacterial actions of the AgNPs and the lincomycin as single entities and as part of the combined mixture of the AgNPs–lincomycin showed improved anti-bacterial biological activity in the Bacillus cereus and Proteus mirabilis microorganisms in comparison to the AgNPs and lincomycin alone. The comparison of the anti-biofilm formation tendency, minimum bactericidal concentration (MBC), and minimum inhibitory concentration (MIC) suggested additive effects of the AgNPs and lincomycin combination co-delivery. The AgNPs’ MIC at 100 μg/mL and MBC at 100 μg/mL for both Bacillus cereus and Proteus mirabilis, respectively, together with the AgNPs–lincomycin mixture MIC at 100 + 12.5 μg/mL for Bacillus cereus and 50 + 12.5 μg/mL for Proteus mirabilis, confirmed the efficacy of the mixture. The growth curve test showed that the AgNPs required 90 min to kill both bacterial isolates. The freshly prepared and well-characterized AgNPs, important for the antioxidant activity levels of the AgNPs material, showed radical scavenging potential that increased with the increasing concentrations. The DPPH’s best activity concentration, 100 μg/mL, which is also the best concentration exhibiting the highest anti-bacterial zone inhibition, was chosen for evaluating the combined effects of the antibiotic, lincomycin, and the AgNPs. Plausible genotoxic effects and the roles of AgNPs were observed through decreased Bla gene expressions in the Bacillus cereus and BlaCTX-M-15 gene expressions in the Proteus mirabilis.
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Taiwo Stephen Okanlawon, Stella Mojisola Adeyemo, and Ibukun Sylvester Agbaje. "Isolation and identification of microorganisms associated with Jollof rice sold at Bukateria in Obafemi Awolowo University, Ile -Ife, Osun State, Nigeria." GSC Biological and Pharmaceutical Sciences 22, no. 1 (January 30, 2023): 178–85. http://dx.doi.org/10.30574/gscbps.2023.22.1.0274.
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This study assessed the microbiological quality of Jollof rice by isolating, identifying and characterizing the microorganisms associated with jollof rice. Six replicate samples were collected from different locations in Obafemi Awolowo University campus, Ile-Ife, Osun-State, Nigeria. Maconkey, Nutrient and Potatoes Dextrose agars were used for the isolation and determination of microbial load. Standard morphological and biochemical tests were carried out for the identification and characterization of isolates. A total of 10 bacteria and 10 fungal species were isolated. The total bacterial count ranged from 3.6×103cfu/g to 1.54×105cfu/g, while the total fungal count ranged from 1.04×104sfu/g to 3.0×105sfu/g. The organisms presumptively identified with the percentage occurrence include; Staphylococcus saprophiticus (20%), Proteus vulgaris (10%), Bacillus subtlis (10%), Proteus mirabilis (10%), Micrococcus varians (10%), Bacillus licheniformis (10%), Bacillus cereus (10%), Bacillus polymyxa (10%), Micrococcus luteus (10%) for bacteria isolates and Aspergillus niger (30%), Aspergillus oryzae (30%) and Aspergillus candidus (40%) for fungi isolates. Good personal hygiene, proper sanitation practice and the use of clean utensils during food preparation are recommended to avoid food poisoning and spoilage.
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Salih, N. A. "Antibacterial effect of nettle (Urtica dioica)." Al-Qadisiyah Journal of Veterinary Medicine Sciences 13, no. 1 (June 30, 2014): 1. http://dx.doi.org/10.29079/vol13iss1art270.
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The antibacterial activity of aqueous and 95% ethanol extracts of nettle leaf was tested against some Gram-negative and Gram-positive bacteria isolated from hospitalized patients by the agar well diffusion method. Staphylococcus aureus, Escherichia coli, Klebsiella spp., Bacillus Subtillus, Proteus spp. Salmonella spp. and Pseudomonas spp. were used. The results indicate that both extracts showed different antibacterial activities which were in favor of ethanolic extract because of more solubility of active ingredient in ethanol than in water. Staphylococcus aureus, Bacillus Subtillus, and Salmonella spp. showed the highest susceptibility to nettle extracts antibacterial effect, while E coli, Pseudomonas and Proteus were less susceptible. The only clear resistant bacteria isolate was Klebsiella spp.
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Raimondi, Stefano, Gloria Spampinato, Laura Ioana Macavei, Linda Lugli, Francesco Candeliere, Maddalena Rossi, Lara Maistrello, and Alberto Amaretti. "Effect of Rearing Temperature on Growth and Microbiota Composition of Hermetia illucens." Microorganisms 8, no. 6 (June 15, 2020): 902. http://dx.doi.org/10.3390/microorganisms8060902.
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The potential utilization of black soldier fly (Hermetia illucens) as food or feed is interesting due to the nutritive value and the sustainability of the rearing process. In the present study, larvae and prepupae of H. illucens were reared at 20, 27, and 33 °C, to determine whether temperature affects the whole insect microbiota, described using microbiological risk assessment techniques and 16S rRNA gene survey. The larvae efficiently grew across the tested temperatures. Higher temperatures promoted faster larval development and greater final biomass but also higher mortality. Viable Enterobacteriaceae, Bacillus cereus, Campylobacter, Clostridium perfringens, coagulase-positive staphylococci, Listeriaceae, and Salmonella were detected in prepupae. Campylobacter and Listeriaceae counts got higher with the increasing temperature. Based on 16S rRNA gene analysis, the microbiota of larvae was dominated by Providencia (>60%) and other Proteobateria (mainly Klebsiella) and evolved to a more complex composition in prepupae, with a bloom of Actinobacteria, Bacteroidetes, and Bacilli, while Providencia was still present as the main component. Prepupae largely shared the microbiota with the frass where it was reared, except for few lowly represented taxa. The rearing temperature was negatively associated with the amount of Providencia, and positively associated with a variety of other genera, such as Alcaligenes, Pseudogracilibacillus, Bacillus, Proteus, Enterococcus, Pediococcus, Bordetella, Pseudomonas, and Kerstersia. With respect to the microbiological risk assessment, attention should be paid to abundant genera, such as Bacillus, Myroides, Proteus, Providencia, and Morganella, which encompass species described as opportunistic pathogens, bearing drug resistances or causing severe morbidity.
Dissertations / Theses on the topic "Bacillus proteus"
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Contesini, Fabiano Jares. "Produção, caracterização e aplicação de proteases de Bacillus sp. = Production, characterization and application of proteases from Bacillus sp." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/254357.
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Orientador: Hélia Harumi SatoTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
Made available in DSpace on 2018-08-26T00:05:53Z (GMT). No. of bitstreams: 1 Contesini_FabianoJares_D.pdf: 2309946 bytes, checksum: 15f6069e97515a200deb29e0577eee62 (MD5) Previous issue date: 2014
Resumo: Proteases bacterianas são enzimas de elevada importância comercial, amplamente aplicadas em diversas áreas como nas indústrias de detergentes, de alimentos, farmacêutica e têxtil. Este trabalho teve como principais objetivos selecionar entre 59 linhagens de Bacillus sp., da coleção de culturas do Laboratório de Bioquímica de Alimentos da FEA, aquelas que apresentam potencial de maior produção de proteases com características tais como estabilidade em diferentes condições de temperatura, pH, detergentes e solventes orgânicos, atividade em ampla faixa de pH e capacidade de lisar células de Xanthomonas campestris. Em seguida, visou-se otimizar a produção de proteases pela linhagem selecionada, determinar as características bioquímicas da protease parcialmente purificada e estudar a aplicação do extrato enzimático bruto e preparação parcialmente purificada. Entre as cinquenta e nove linhagens de Bacillus sp. testadas foram selecionadas nove linhagens que produziram maior atividade de proteases. A produção de protease pelas nove linhagens foi testada em frascos agitados contendo o meio de cultura nº 1 (10g/L de caseína, 1g/L de extrato de levedura e sais), meio nº 2 (35 g/L de melaço de cana de açúcar, 20g/L de água de maceração de milho, 3g/L de extrato de levedura Prodex-Lac SD® e 20g/L de soro de queijo), e por fermentação em meio sólido nº 3 (farelo de trigo e água, na proporção 1:1, m:m). As linhagens de Bacillus sp. LBA 07, Bacillus sp. LBA 46 e Bacillus sp. LBA 08 fermentadas nos meios de cultura nº 1, nº 2 e nº 3 produziram 222 U/mL, 548 U/mL e 13480 U/grama de substrato seco (gss) respectivamente. As proteases dos extratos enzimáticos brutos obtidos das nove linhagens fermentadas nos três meios de cultura apresentaram atividade ótima na faixa de pH 7 a 9 e 60° C, estabilidade na faixa de pH 5 a 9 por 24h a 4º C , e após 1 h de tratamento a 50° C. Entre os extratos enzimáticos brutos de proteases testados, aqueles obtidas da fermentação de Bacillus sp. LBA 46 nos três meios de cultura foram as mais estáveis em detergente Ariel®. Quando incubadas em solventes orgânicos alguns extratos enzimáticos brutos proteases mantiveram mais de 60% de atividade residual após 24h em acetona (Bacillus sp. LBA 8 e 44), hexano (Bacillus sp. LBA 19, 29, 44, 46 e 60), clorofórmio (Bacillus sp. LBA 44 and 60) e etanol (Bacillus sp. LBA 60). Os extratos enzimáticos brutos de proteases obtidos do cultivo da linhagem de Bacillus sp. LBA 46 nos meios n° 2 e n° 3 foram as mais eficientes na lise de células de Xanthomonas campestris, aumentando cerca de 30% a transmitância a 620 nm (Trans 620nm) do meio fermentado de goma xantana. A linhagem de Bacillus sp. LBA 46 foi selecionada como melhor produtora de protease e estudos preliminares de identificação biomolecular indicam que se trata de uma linhagem de Bacillus licheniformis. Utilizando-se a linhagem de Bacillus sp LBA 46 e o meio de cultura otimizado (meio n° 4) por metodologia de superfície de resposta (MSR), composto de 40g/L de melaço de cana de açúcar, 6g/L de água de maceração de milho, 2g/L de extrato de levedura Prodex-Lac SD® e 20g/L de soro de queijo, foi obtido 3000 U/mL de protease após 96h de fermentação a 30° C e 200 rpm. No estudo da aplicação da enzima para a remoção de manchas de tecidos de algodão foram obtidos melhores resultados de remoção de manchas de sangue e molho de tomate com carne moída, utilizando-se a combinação de extrato bruto de protease (100 ou 1000U) com o detergente Omo®. O extrato enzimático bruto da linhagem de Bacillus sp. LBA 46 foi parcialmente purificado por fracionamento com sulfato de amônio (80% de saturação), diálise e cromatografia de filtração em gel (Sephadex G100), resultando em fator de purificação de 3,69. Após caracterização com MSR observou-se que a protease da preparação parcialmente purificada apresentou atividade ótima a 55° C e pH 7,5 e considerável estabilidade (95% de atividade residual) na faixa de pH 5,7 ¿ 9,3 após 1h de incubação a 30 ¿ 36° C, e acima de 78,9% quando incubadas por 1h em pH 7,5 e 50° C. A condição ótima de lise das células de X. campestris do meio fermentado de goma xantana utilizando-se o extrato enzimático bruto de protease e a preparação parcialmente purificada de proteases, foi observada utilizando 42 U de protease /mL de suspensão celular de X. campestris a 60° C, resultando em aumento de mais de 20% da Trans 620nm do meio fermentado de goma xantana. Um aumento de quase 40% de Trans 600nm foi observado após 2h de reação utilizando extrato enzimático bruto de protease (42 U de protease/mL de suspensão celular de X. campestris) a 65° C. A produção de proteases de Bacillus sp. LBA 46 por fermentação em estado sólido foi otimizada utilizando MSR, sendo obtido 5000 U/grama de substrato seco utilizando-se meio de cultura composto de farelo de trigo e água (60%:40%) após 96h de fermentação a 30° C
Abstract: Proteases are commercially relevant enzymes widely applied in several industrial areas, such as in detergent, food, pharmaceutical and textile industries. Proteases from Bacillus sp. can present advantages compared to the proteases from other sources, including better thermostability, stability in pH range from slightly acid to alkaline pH values and stability in organic solvents. The aims of this work were selecting Bacillus sp. strains with capability of producing proteases with better biochemical properties, such as stability in different conditions of temperature, pH, detergents and organic solvents, activity in a wide range of pH and capability of lysing cells of Xanthomonas campestris. Afterwards, it was aimed the optimization of the production of proteases by the selected Bacillus sp. strain and the determination of the biochemical characteristics of the partially purified protease and the application of the crude and partially purified protease. Nine Bacillus sp. strains were selected as the best protease producers among fifty nine Bacillus sp. strains tested. The protease production by the nine strains was carried out in Erlenmeyer flasks containing medium no. 1 (10g /L of casein, 1g/L of yeast extract and salts), medium no. 2 (35 g/L of sugar cane molasses, 20g/L corn steep liquor, 3g/L of yeast extract Prodex-Lac SD® and 20g/L of dried whey), e by fermentation using solid substrate medium no. 3 (wheat bran and water, 1:1, m:m). The strains Bacillus sp. LBA 07, Bacillus sp. LBA 46 and Bacillus sp. LBA 08 when fermented in medium no. 1, no. 2 e no. 3 produced 222 U/mL, 545 U/mL and 13480 U/gram of dried substrate (gds) respectively. Proteases from the crude enzymatic extracts obtained from the fermentation of the nine Bacillus sp. strains in the three media showed optimal activity in pH range 7-9 and 60° C, stability in pH range 5-9 for 24 hours at 4° C and after 1h at 50° C. The protease preparations from the fermentation of Bacillus sp. LBA 46 in the three media were the most stable when incubated in detergent Ariel®, among the proteases tested from the Bacillus sp. strains. In addition, some proteases presented more than 60% residual activity after 24h in the organic solvents acetone (Bacillus sp. LBA 8 and 44), hexane (Bacillus sp. LBA 19, 29, 44, 46 and 60), chloroform (Bacillus sp. LBA 44 and 60) and ethanol (Bacillus sp. LBA 60). The protease preparations obtained from the cultivation of Bacillus sp. LBA 46 in medium no. 2 and no. 3 presented the best results on the lysis of Xanthomonas campestris cells, resulting in an increase of approximately 30% in transmittance at 620 nm (Trans 620nm) of the fermented broth of xanthan. Bacillus sp. LBA 46 strain was selected as the best protease producer and after preliminary biomolecular analysis of identification, the results indicate that this microorganism correspond to a Bacillus licheniformis strain. Protease preparation containing 3000 U/mL was obtained from Bacillus sp. LBA 46 cultivated in Erlenmeyer flasks containing medium no. 4 composed of 40g/L of sugar cane molasses, 6g/L of corn steep liquor, 2g/L of yeast extract Prodex-Lac SD® and 20 g/L of dried whey after 96h of fermentation at 30° C and 200 rpm, optimized with response surface methodology (RSM). In the the washing tests, the best results of the removal of blood and tomato sauce with ground beef stains from cotton fabrics were observed using the combination of crude extract of protease (100 or 1000U) with detergent Omo®. Crude protease extract of the Bacillus sp. LBA strain was partially purified by ammonium sulfate fractionation (80% saturation), dialysis and gel filtration chromatography (Sephadex G100), resulting in the purification fold of 3.69. After characterization with RSM it was observed that the crude protease extract and partially purified proteases presented optimal activity at 55° C and pH 7.5 and considerable stability (95% of residual activity) in pH range 5.7 ¿ 9.3 after 1h incubation at 30-36° C and more than 78.9% when incubated at pH 7.5 and 50 °C for 1h. The optimal conditions of the lysis of X. campestris cells contained in the fermentation broth using crude and partially purified protease preparations were observed using 42 U of protease/mL of cell suspension of X. campestris at 60° C, resulting in a increase of more than 20% in Trans 620 nm of the fermented broth of xanthan. It was observed an increase of almost 40% in Trans 620 nm after 2h reaction using crude protease (42 U de protease/mL of cell suspension of X. campestris) at 65° C. The production of proteases by Bacillus sp. LBA 46 under solid state fermentation was optimized using RSM, resulting in 5000 U/gram of dry substrate utilizing wheat bran and water (6g:4g) after 96h of fermentation at 30° C
Doutorado
Ciência de Alimentos
Doutor em Ciência de Alimentos
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McNeil, Betina C. "Mutational Analysis and Characterization of Microbial Pesticides Isolated from Bacillus Thuringiensis." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1316527600.
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Carmel, Andrew Barry. "Crystal structure of BstDEAD, a novel DEAD-box protein from Bacillus stearothermophilus /." view abstract or download file of text, 2003. http://wwwlib.umi.com/cr/uoregon/fullcit?p3095239.
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Thesis (Ph. D.)--University of Oregon, 2003.Typescript. Includes vita and abstract. Includes bibliographical references (leaves 101-114). Also available for download via the World Wide Web; free to University of Oregon users.
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Thwaite, Joanne E. "Factors influencing the production of Bacillus anthracis protective antigen in Bacillus subtilis." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369784.
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Walker, Dianne. "Bacillus stearothermophilus pyruvate kinase." Thesis, University of Bristol, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335572.
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Truong, Hung Phuc. "Fate of Cry Toxins from Bacillus thuringiensis in soil." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS210.
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Les propriétés insecticides du Bacillus thuringiensis, découvert par ShigentaneIshiwatari, ont été utilisées pendant des décennies comme biopesticides et cette utilisation a augmenté rapidement en raison de préoccupations au sujet des effets environnementaux négatifs des pesticides chimiques. Actuellement, la toxine Bt dans la forme de biopesticides et des plantes transgéniques Bt peut compléter ou remplacer les pesticides chimiques. Il y a peu d’indication que la toxine Bt a un effet nocif pour l'environnement ou la santé humaine. Néanmoins, il ya des préoccupations que les cultures transgéniques commerciales peuvent avoir des effets néfastes sur l'environnement. Après son introduction dans le sol l'exsudation racinaire et la dégradation des résidus végétaux, la toxine Bt interagit avec les particules de sol. Les interactions de la toxine Bt avec des particules de sol influencent sa mobilité, sa biodisponibilité, sa persistance et sa toxicité.Dans cette étude, nous visons à établir l'importance relative des facteurs biologiques et physico-chimiques dans la détermination de la dynamique des protéines Cry détectables dans les sols, de clarifier si la protéine adsorbée conserve ses propriétés insecticides et d'identifier les propriétés du sol qui déterminent le devenir des protéines Cry dans le sol. Les résultats montrent que les protéines Cry ont une forte affinité sur la surface du sol. Cependant, il y avait peu de relation entre l'affinité pour le sol ou le rendement d'extraction et les propriétés du sol, y compris la teneur en argile, teneur en carbone organique et le pH du sol. Il y avait peu de rapport entre l'affinité et le rendement d'extraction. Les protéines diffèrent à la fois dans leur affinité pour les sols et leurs rendements d'extraction.Une évaluation du rôle du sol et des facteurs environnementaux dans le sort des protéines Cry de la formulation de biopesticides commerciale a montré un déclin rapide de la protéine Cry détectable soumise aux rayons du soleil sous la condition de laboratoire, alors que peu d'effet a été observé dans des conditions de terrain. La demi-vie des protéines dans le sol dans des conditions naturelles était d'environ 1 semaine. Des effets de la température forts ont été observés, mais ils diffèrent pour les biopesticides et la protéine purifiée, indiquant différentes étapes limitantes. Pour le biopesticide, la baisse observée était ralenties par des facteurs biologiques, y compris éventuellement sporulation. En revanche pour des protéines purifiées, augmentation de la température améliorée des changements conformationnels de la protéine adsorbée du sol, conduisant à une fixation et, par conséquent diminué efficacité d'extraction qui a diminué avec le temps. En outre, l'étude de la persistance de diverses protéines Cry dans les sols contrastés a été réalisée par immuno-détection et dosage biologique a montré que la toxine extractible diminue avec incubation allant jusqu'à quatre semaines. L'activité insecticide était toujours maintenue à l'état adsorbé, mais a disparue après deux semaines d'incubation à 25°C. La baisse de la protéine extractible et la toxicité était beaucoup plus faible à 4°C à 25°C. La stérilisation du sol n'a pas eu d'effet significatif sur la persistance de la toxine Cry indiquant que le déclin observé était provoqué par la fixation en fonction du temps de la protéine adsorbée ce qui diminue la quantité de toxine Cry extractable, la dégradation de la protéine par l’activité microbienne jouant un rôle plus mineur.L’exposition des insectes aux protéines Cry sous la forme adsorbé pourrait avoir un impact significatif sur les insectes cibles et même les insectes non cibles, et devrait être plus étudiée afin de déterminer son impact potentielThe insecticidal properties of Bacillus thuringiensis, discovered by Shigentane Ishiwatari, have been used for decades as biopesticides and this use has been increasing rapidly because of concerns about the negative environmental effects of chemical pesticides. Currently, Bt toxin in the form of both biopesticides and Bt transgenic plantsmay supplement or replace chemical pesticide. There is little evidence to demonstrate that Bt toxin has any harmful effect to the environment or to human health. Nevertheless, there are concerns that commercial transgenic crops may have harmful impacts on the environment. After release into soil via root exudation and breakdown of plant residues, Bt toxin interacts with soil particles. The interactions of Bt toxin with soil particles influence its mobility, its bioavailability, its persistence and its toxicity. In this study, we aim to establish the relative importance of biological and physicochemical factors in the determination of the dynamics of detectable Cry proteins in soils, to clarify if adsorbed protein maintains its insecticidal properties and to identify the soil properties that determine the fate of Cry proteins in soil. The results show that Cry proteins have strong affinity on soil surface. However, there was little relationship between affinity for soil or the extraction yield and soil properties including clay content, organic carbon content and soil pH. There was little relationship between the affinity and the extraction yield. The proteins differ in both their affinity for soil and their extraction yields.An assessment of role of soil and environmental factors in the fate of Cry protein from commercial biopesticide formulation showed a rapid decline of detectable Cry protein subjected to direct sunlight under the laboratory condition, whereas, little effect was observed under field conditions. The half-life of proteins in soil under natural conditions was about one week. Strong temperature effects were observed, but theydiffered for biopesticide and purified protein, indicating different limiting steps. For biopesticide, the observed decline was due to biological factors, possibly including sporulation. In contrast for purified proteins, increased temperature enhanced conformationalchanges of the soil-adsorbed protein, leading to fixation and hence extraction efficiency decreased that decreased with time. Moreover, the study of persistence of various Cry proteins in contrasting soils was carried out by immuno-detection and bioassay showed that extractable toxin decreased with incubation of up to four weeks. Insecticidal activity was still retained in the adsorbed state, but lost after two weeks of incubation at 25°C. The decline in extractable protein and toxicity was much lower at 4°C than 25°C. There was no significant effect of soil sterilization to persistence of Cry toxin indicating that decrease in detectable Cry toxin in soil may be time-dependent fixation of adsorbed protein as well as decreasing solubilization in larva midgut, but not microbial breakdown.Exposition to Cry in the adsorbed form could have a significant impact on target and even non target insects and should be investigation to determine the potential impact
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Shakir, Salika Mehreen. "Characterization of a serine/threonine phosphatase-kinase pair in Bacillus anthracis." Oklahoma City : [s.n.], 2010.
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Dürrschmidt, Peter. "Entfaltung und Autoproteolyse der neutralen Protease aus Bacillus stearothermophilus und einer Disulfid-modifizierten Variante." [S.l. : s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=970184883.
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Xue, Yong. "Effects of Protein Domains on Localization of Penicillin-Binding Proteins 2a and 2b in Bacillus Subtilis." Thesis, Virginia Tech, 2008. http://hdl.handle.net/10919/35255.
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Peptidoglycan not only protects bacterial cells against intracellular pressure but also provides the cells with a defined morphology. Penicillin-binding proteins (PBPs) catalyze the polymerization of the peptidoglycan in Bacillus subtilis. PBP2a and PBP2b are class B PBPs which have been known to have transpeptidase activities and they localize at different positions on the cell membrane. PBP2a spreads around the cylindrical wall as well as some at the septum, and PPB2b localizes exclusively to the septum and some at the cell poles. Both PBP2a and PBP2b are composed of four domains: S, N, P, and C domains from the N- to C- terminus. A FLAG epitope was tagged to the C-terminal ends of PBP2a and PBP2b. Cells with FLAG tagged PBP2a or PBP2b grow as well as wild type strain. Expression of PBP2a-FLAG and PBP2b-FLAG can be detected by western blotting using anti FLAG antibody. The expression of wild type PBP2a/PBP2b in these strains was tightly controlled by a xylose promoter. The FLAG fusion didnâ t influence the normal membrane localizations of PBP2a or PBP2b.
PBP2a/2b mutant strains with the S and/or N domains switched between PBP2a and PBP2b were constructed. All these domain-switch proteins were tagged with a FLAG at the C-terminus. The expression of these recombinant proteins can be detected by western blotting. None of these domain-switch proteins was able to complement the wild type PBP2a and PBP2b and cells with only these recombinant proteins but no wild type proteins were non-viable. Cellular localization of these domain switch proteins were visualized using immunofluorescence microscopy. Proteins containing the PBP2a S domain had the same localization patterns as wild type PBP2a. Proteins that have the PBP2b S domain localized specifically at the septum and cell poles, which is similar to the wild type PBP2b. These results indicate that the S domain is the determinant to direct PBP2a and PBP2b to their cellular destinations.Master of Science
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Rawlings, Andrea Elizabeth. "Structural investigations of proteins from Bacillus." Thesis, University of York, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.489200.
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For many years the Gram positive bacterium Bacillus subtilis has been a model organism for prokaryotic cell and molecular biology. The asymmetric cell division which B. subtilis undergoes during sporulation is a simple system by which to study the process of cell differentiation. This process is governed by a series of genetic, temporal and spatial controls. Gene regulation, brought about by a series of a factors and transcriptional regulators, is coupled to key morphological stages or checkpoints.
Books on the topic "Bacillus proteus"
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Temeyer, Kevin Bruce. Monoclonal antibodies to crystal protein of Bacillus thuringiensis subspecies Israelensis. [Washington, D.C.?: U.S. Dept. of Agriculture?], 1987.
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Feijoo, Sergio C. Rapid assay for Bacillus proteinases in raw milk as detected by a simple casein denaturation method. 1991.
Find full textBook chapters on the topic "Bacillus proteus"
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Schnepf, H. Ernest. "Bacillus thuringiensis Recombinant Insecticidal Protein Production." In Bacillus thuringiensis Biotechnology, 259–81. Dordrecht: Springer Netherlands, 2012. http://dx.doi.org/10.1007/978-94-007-3021-2_14.
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Florez, Alvaro M., Cristina Osorio, and Oscar Alzate. "Protein Engineering of Bacillus thuringiensis δ-Endotoxins." In Bacillus thuringiensis Biotechnology, 93–113. Dordrecht: Springer Netherlands, 2012. http://dx.doi.org/10.1007/978-94-007-3021-2_5.
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Eggert, Thorsten, Susanne A. Funke, Jennifer N. Andexer, Manfred T. Reetz, and Karl-Erich Jaeger. "Evolution of Enantioselective Bacillus subtilis Lipase." In Protein Engineering Handbook, 441–51. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2011. http://dx.doi.org/10.1002/9783527634026.ch17.
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Li, Jade. "Insecticidal δ-Endotoxins from Bacillus Thuringiensis." In Protein Toxin Structure, 49–77. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-662-22352-9_4.
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Nagarajan, Vasantha. "Protein Secretion." In Bacillus subtilis and Other Gram-Positive Bacteria, 713–26. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555818388.ch49.
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Errington, Jeffery, and Andrew Mountain. "Is Bacillus an Alternative Expression System?" In Protein Production by Biotechnology, 1–14. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-1565-0_1.
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Setlow, Peter. "Spore Structural Proteins." In Bacillus subtilis and Other Gram-Positive Bacteria, 801–9. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555818388.ch55.
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Aronson, Arthur I. "Formation and Properties of a Bacillus Subtilis Protein Protease Inhibitor." In Extracellular Enzymes of Microorganisms, 93–98. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4684-1274-1_12.
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van Frankenhuyzen, Kees. "Specificity and Cross-order Activity of Bacillus thuringiensis Pesticidal Proteins." In Bacillus thuringiensis and Lysinibacillus sphaericus, 127–72. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-56678-8_10.
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Bravo, Alejandra, Sabino Pacheco, Isabel Gómez, Blanca Garcia-Gómez, Janette Onofre, and Mario Soberón. "Insecticidal Proteins from Bacillus thuringiensis and Their Mechanism of Action." In Bacillus thuringiensis and Lysinibacillus sphaericus, 53–66. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-56678-8_4.
Full textConference papers on the topic "Bacillus proteus"
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Barros, Alana Maria Tavares, Nilo Ricardo Vasconcelos Torquarto, Millena Marinho Santos, Ana Beatriz Pires Curvelo Martins Tenório Carmo, Roberto Rômulo Ferreira Da Silva, and Rodrigo Antônio Torres Matos. "OCORRÊNCIA DE OTITE EXTERNA EM CÃES E GATOS ATENDIDOS EM UMA CLÍNICA ESCOLA DE MEDICINA VETERINÁRIA." In I Congresso On-line Nacional de Clínica Veterinária de Pequenos Animais. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1893.
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Introdução: A otite externa caracteriza-se como uma inflamação aguda ou crônica do epitélio do meato auditivo, sendo uma das afecções mais comuns na rotina da clínica médica dos animais. Algum dos fatores que influenciam no aparecimento das otites são: morfologia das orelhas, limpeza excessiva do conduto auditivo, doenças sistêmicas e alterações climáticas. Objetivos: Objetivou-se com este trabalho, realizar um estudo retrospectivo da ocorrência de otite em cães e gatos atendidos na clínica escola de Medicina Veterinária. Material e métodos: No período de 2019 a 2021, foi realizado a partir de análises das fichas clínicas um levantamento dos casos diagnosticados de otite de cães e gatos atendidos na clínica médica de pequenos animais do Centro Universitário Cesmac, Marechal Deodoro, Alagoas. Foram analisados a espécie, sexo e raça dos animais acometidos, realizando a identificação dos microrganismos em ambos ouvidos, feita por exame microbiológico, com auxílio de swab estéril e meio de transporte Stuart. Resultados: Foram colhidas 26 amostras de secreção de conduto auditivo esquerdo e direito de 15 animais (13 cães e 2 gatos). Destes 13 cães, 53,84% (7/13) eram fêmeas; 46,16% (6/13) eram machos, dos 2 gatos, 100% (2/2) eram machos. Dos 13 cães, 46,15% (6/13) eram SRD; 23,07% (3/13) eram Dachshund; 15,38% (2/13) eram Pastor-Alemão; 07,69% (1/13) eram Pug e 07,69% (1/13) eram Labrador Retriever; dos 2 gatos, 100% (2/2) eram SRD. Do total de amostras avaliadas, foram observados os microrganismos Streptococcus sp. 15,38% (4/26); 11,53% (3/26) Staphylococcus sp + Klebisiella sp; 11,53% (3/26) Proteus sp + Staphylococcus sp; 11,53% (3/26) Bacillus sp; 07,69% (2/26) Pseudomonas sp; 07,69% (2/26) Staphylococcus sp + Streptococcus sp; 07,69% (2/26) Proteus sp; 07,69% (2/26) Bacillus sp + Staphylococcus sp; 07,69% (2/26) Malassezia pachydermatis; 07,69% (2/26) Pseudomonas sp. + Proteus sp + Staphylococcus sp; 03,84% (1/26) Proteus sp + Pseudomonas sp. Conclusão: Diante dos resultados, foi possível observar que o agente mais prevalente foi Streptococcus sp. Houve uma maior ocorrência de infecções em cães quando comparado com os gatos, não sendo observada predileção sexual. Ressalta-se a importância da realização da cultura e antibiograma para que seja feito o tratamento mais adequado para combater a infecção.
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Stanković, Marina M., Jelena Z. Pribojac, Jelena N. Terzić, and Olgica D. Stefanović. "EFFECT OF PLANT EXTRACTS ON BACTERIAL GROWTH AND POTENTIAL MECHANISM OF ACTION." In 1st INTERNATIONAL Conference on Chemo and BioInformatics. Institute for Information Technologies, University of Kragujevac, 2021. http://dx.doi.org/10.46793/iccbi21.343s.
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Mentha piperita and Melissa officinalis are both well-known medicinal plants that have applications in traditional medicine. In this research the antibacterial activity of the ethanol extracts of M. piperita and M. officinalis was examined against 14 bacterial strains via the microdilution method. Minimum inhibitory concentrations of ethanol extracts of both plant species ranged from 0.312 to 20 mg/mL. Standard strains of Staphylococcus aureus ATCC 25923 at a concentration of 0.312 mg/mL and Bacillus subtilis ATCC 6633 at a concentration of 1.25 mg/mL showed the highest sensitivity to the ethanol extract of M. piperita. Ethanol extract of M. officinalis showed antibacterial activity on standard strains of S. aureus ATCC 25923 and B. subtilis ATCC 6633 at a concentration of 0.625 mg/mL. In addition to the mentioned standard strains, it showed activity on the isolate from the food Proteus spp. at a concentration of 0.312 mg/mL and isolate from the wound Proteus mirabilis at a concentration of 0.625 mg/mL. Mechanism of action of the ethanol extract of M. officinalis was examined on the permeability of the bacterial cell membrane. The effect of the extract on the increased permeability of the cell membrane was measured based on the release of proteins and the percentage of crystal violet binding. Ethanol extract of M. officinalis has been shown to act at the level of the cell membrane in the following bacterial strains of Pseudomonas aeruginosa, S. aureus and Enterococcus spp.
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Motta, Nicollas tomas de Aquino, Valeria Bentes Ferreira, Edgleidson Silva Dos Santos, Rodrigo Antonio Torres Matos, Fabricia Duarte Omena, and Francielly Gomes Vilas Boas. "ETIOLOGIA DAS INFECÇÕES DO TRATO URINÁRIO (ITU) DE CÃES E GATOS." In I Congresso On-line Nacional de Clínica Veterinária de Pequenos Animais. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1863.
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Introdução: As infecções bacterianas são de grande importância sendo verificada com maior ocorrência a infecção urinária. Objetivo: Objetivou-se com presente trabalho identificar os principais organismos isolados de amostras clinicas de cães e gatos com infecção urinaria atendidos em clinicas veterinárias nos municípios de Arapiraca e Maceió-AL. Material e métodos: No período de março a junho de 2021, realizou-se um levantamento das fichas e laudos de cães e gatos dos casos de infecções urinárias, obtidos no Centro de Diagnostico Veterinário (VETLAB). A partir das analises dos arquivos, obteve-se dados como raça, sexo e idade. Além disso, foi possível identificar os micro-organismos isolados por meio de amostras colhidas. Resultados: Os atendimentos realizados foram em 15 animais, sendo doze cães 80% (12/15), estes possuindo idades entre um a dezesseis anos, com predominância de animais das raças Shih-Tzu 58,33% (7/12), seguindo das raças Pug 25% (3/12), Yorkshire 8,33% (1/12) e SRD 8,33% (1/12). E três felinos 20% (3/15), com idade entre dois a dezessete anos; raça SRD que tiveram predominância de 100%. Destes 15 animais, foram colhidas amostras do trato urinário para avaliação de predominância bacteriana. Nesse ponto, houve maior ocorrência de Staphylococcus sp. 40% (6/15), Proteus sp. 20% (3/15), Bacillus sp. 13,33% (2/15) e Streptococcus + proteus sp. 6,67% (1/15) . Em apenas três amostras não houve o crescimento bacteriano. Conclusão: Diante dos resultados, observou-se que as bactérias do gênero Staphylococcus sp. e Proteus sp., foram as mais prevalentes nas afecções urinárias. Devido ao aumento das infeções bacterianas, ressalta-se a importância de realizar a urinálise, além disso, cultura e antibiograma com intuito de instituir o tratamento mais adequado.
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H. Shareef, Suhayla, Chiman H. Saeed, and Pshtiwan D. Majeed. "Antimicrobial activity of Syzigium Aromaticum (Clove) and Salvadora Persica (Miswak) Against Dental Plague Pathogens." In 4th International Conference on Biological & Health Sciences (CIC-BIOHS’2022). Cihan University, 2022. http://dx.doi.org/10.24086/biohs2022/paper.626.
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Some periodontal bacteria on tooth plaque were shown to be sensitive to extracts from Syzigium aromaticum and Salvadora persica. Our study was an assessment of the antimicrobial effect of Syzigium aromaticum and Salvadora persica extracts against dental plague pathogens. Thirty-five 35 oral swabs were taken from the patients who had the dental plague and isolated microorganisms were identified by standard bacteriological methods. Syzigium aromaticum and Salvadora persica extracts were tested against dental plaque microorganisms on Muller Hinton agar, antimicrobial activity of two extracts were done by paper disks-diffusion technique at 50% concentration and characterized by inhibition zones. Forty-six (46) microorganisms were isolated from 35 dental plague samples,18 isolates were Staphylococcus aureus, 3 isolates were Staphylococcus epidermidis, 6 isolates were Streptococcus mutans, 12 isolates were Streptococcus viridans, 1 isolate each of Pseudomonas aeruginosa., Proteus spp., Bacillus spp., 2 isolates were Actinobacillus actinomycetemcomitans and 2 isolates were Candida albicans. The zones of inhibition for two different extracts were measured. According to the findings, Syzigium aromaticum and Salvadora persica have antibacterial and antifungal effects against gram-negative and gram-positive bacteria, as well as fungi. Our study has shown that Syzigium aromaticum and Salvadora persica extracts have antimicrobial efficacy, and concluded that the extracts have shown a greater effect on oral microorganisms and are recommended to be used for treatment and prevention against oral hygiene.
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Carmo, Ana Beatriz Pires Curvelo Martins Tenório, Nilo Ricardo Vasconcelos Torquato, Millena Marinho Santos, Alana Maria Tavares Barros, Roberto Rômulo Ferreira Da Silva, and Rodrigo Antônio Torres Matos. "LEVANTAMENTO DE AGENTES BACTERIANOS ISOLADOS DE AMOSTRAS DE CÃES E GATOS COM AFECÇÕES CLÍNICAS." In I Congresso On-line Nacional de Clínica Veterinária de Pequenos Animais. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1929.
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Introdução: Infecções bacterianas são de grande importância na rotina clínica de pequenos animais, sendo as mais comuns, as infecções de conduto auditivo e infecções urinárias. Objetivo: Objetivou-se com este trabalho verificar os agentes das afecções clínicas em cães e gatos atendidos na Clínica Escola de Medicina Veterinária. Material e métodos: No período de fevereiro de 2020 a junho de 2021, realizou-se um levantamento das fichas dos casos de afecções clínicas em cães e gatos atendidos na Clínica Escola do Centro Universitário Cesmac, Marechal Deodoro, Alagoas. A partir das análises das fichas, obteve-se dados como raça, sexo, idade, além disso foi possível identificar os principais microrganismos isolados por meio de exame microbiológico das amostras colhidas. Resultados: Neste período foram atendidos 11 animais, sendo seis cães (54,54%) e cinco felinos (45,46%). Os cães possuíam idades entre um mês a 10 anos, e gatos predominantemente jovens de quatro meses a três anos. No agrupamento das raças, os cães tiveram predominância de animais SRD (33,33%) seguindo das raças Pug (16,66%), Labrador Retriever (16,66%), Dachshund (16,66%) e Pastor-alemão (16,66%). Já os felinos SRD. tiveram predominância de 100%. Destes 11 animais, foram colhidas 17 amostras (11 amostras de secreção otológica, duas amostras de trato urinário, uma de liquido intracraniano, uma de secreção nasal, uma de aspirado de lesão e uma de fragmento de pele). As infecções de conduto auditivo e trato urinário foram as mais observadas na rotina da clínica médica. Houve maior ocorrência de Staphylococcus sp. 23,52% (4/17) e Streptococcus sp. 23,52% (4/17) nas amostras clínicas, seguido de Staphylococcus + Proteus sp. + Pseudomonas 11,76% (2/17); Bacillus sp. 11,76% (2/17); Staphylococcus sp. + Streptococcus sp. 5,88% (1/17) ; Streptococcus sp. + Proteus sp. 5,88% (1/17); e bactéria Gram Negativa 5,88% (1/17). Em apenas duas amostras não houve o crescimento bacteriano, sendo observada a presença do fungo Malassezia sp (11,76%). Conclusão: Observou-se que as bactérias Staphylococcus sp. e Streptococcus sp. foram as mais prevalentes nas afecções clínicas. Devido ao aumento das infeções bacterianas, ressalta-se a importância de realizar a cultura bacteriana e o antibiograma, com intuito de instituir o tratamento mais eficaz para cada tipo de afecção.
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Reichert, Thaynara, and Keila Zaniboni Siqueira Batista. "PROPRIEDADES IMUNOLÓGICAS DO MEL DE ABELHAS SEM FERRÃO – REVISÃO BIBLIOGRÁFICA." In I Congresso Brasileiro de Imunologia On-line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/966.
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Introdução: A criação das melíponas ou popularmente conhecidas como “abelhas sem ferrão” e a utilização do mel e seus derivados como recurso terapêutico para prevenir e tratar enfermidades, é uma prática antiga, com relatos desde os primórdios da civilização egípcia. A crescente busca e estudos realizados acerca do mel produzido por abelhas sem ferrão ocorre devido à sua composição química relacionada à efeitos antissépticos, antimicrobianos, anti-inflamatórios, anticancerígenos e cicatrizantes. Objetivos: Revisar a literatura dos últimos 5 anos, sobre as propriedades imunológicas, utilização e os benefícios do mel de abelhas sem ferrão. Material e métodos: a busca da literatura foi realizada em plataformas como Pubmed, SciELO e Portal CAPES, usando as palavras-chave “abelhas sem ferrão”, “mel” e “propriedades bioativas”. Resultados: O mel de abelhas sem ferrão possui alto teor de açúcares, baixo pH, sais minerais, vitaminas, e em menores quantidades proteínas e flavonoides. Tais compostos, conferem ao mel, atividade antimicrobiana inibitória contra bactérias do gênero Staphylococcus aureus, Bacillus sp, Proteus spp., Escherichia coli e Pseudomonas aeruginosa. Além da alta viscosidade do mel que proporciona uma barreira física em feridas capaz de evitar a exsudação e neutralizar intermediários reativos do oxigênio. Seu uso experimental em feridas contaminadas de ratos induziu a formação de granulações no tecido, estimulou o crescimento epitelial e a síntese de colágeno reduzindo o tempo de cicatrização; ademais estimulou a produção das citocinas TNF-α, IL1β e IL-6. O mel pode ser empregado no tratamento de doenças que envolvam o estresse oxidativo, uma vez que se mostrou eficaz na captura direta do radical livre DPPH e AAPH, e apresentou atividade anticancerígena inibindo a expressão da proteína inflamatória ICAM-1 e VCAM-1 em células endoteliais. Conclusão: Observa-se, com esses dados, que o mel de abelhas sem ferrão é rico em compostos bioativos que melhoram a resposta imunológica frente a bactérias patogênicas, reações inflamatórias estimulando a produção de citocinas e contribuem no tratamento e prevenção de enfermidades relacionas ao estresse oxidativo, sugerindo o desenvolvimento de novas linhas de pesquisa para avaliar sua aplicação.
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Rajesh, T. S., Rajan Kr Pande, Preenon Bagchi, and M. Mahesh. "Comparative study and characterisation of Serine protease from Bacillus lichiniformis (MTCC) and Bacillus megaterium." In 2011 3rd International Conference on Electronics Computer Technology (ICECT). IEEE, 2011. http://dx.doi.org/10.1109/icectech.2011.5941723.
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Ibragimov, A., An Baymiev, and O. Lastochkina. "Development of fluorescent protein-marked strains of Bacillus subtilis." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.104.
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Narva, Kenneth. "Bacillus thuringiensisinsecticidal proteins for control ofDiabrotica virgifera virgifera." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.94886.
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FADANNI, O., D. F. REIS, A. BRANDELLI, and S. J. KALIL. "IMOBILIZAÇÃO DE UMA PROTEASE OBTIDA DE BACILLUS SP. P45." In XI Congresso Brasileiro de Engenharia Química em Iniciação Científica. São Paulo: Editora Edgard Blücher, 2015. http://dx.doi.org/10.5151/chemeng-cobeqic2015-345-33949-260745.
Full textReports on the topic "Bacillus proteus"
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Arnett, Clint, Justin Lange, Ashley Boyd, Martin Page, and Donald Cropek. Expression and secretion of active Moringa oleifera coagulant protein in Bacillus subtilis. Engineer Research and Development Center (U.S.), August 2021. http://dx.doi.org/10.21079/11681/41546.
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Cationic polypeptide proteins found in the seeds of the tropical plant Moringa oleifera have coagulation efficiencies similar to aluminum and ferric sulfates without their recalcitrant nature. Although these proteins possess great potential to augment or replace traditional coagulants in water treatment, harvesting active protein from seeds is laborious and not cost-effective. Here, we describe an alternative method to express and secrete active M. oleifera coagulant protein (MO) in Bacillus subtilis. A plasmid library containing the MO gene and 173 different types of secretory signal peptides was created and cloned into B. subtilis strain RIK1285. Fourteen of 440 clones screened were capable of secreting MO with yields ranging from 55 to 122 mg/L of growth medium. The coagulant activity of the highest MO secreting clone was evaluated when grown on Luria broth, and cell-free medium from the culture was shown to reduce turbidity in a buffered kaolin suspension by approximately 90% compared with controls without the MO gene. The clone was also capable of secreting active MO when grown on a defined synthetic wastewater supplemented with 0.5% tryptone. Cell-free medium from the strain harboring the MO gene demonstrated more than a 2-fold reduction in turbidity compared with controls. Additionally, no significant amount of MO was observed without the addition of the synthetic wastewater, suggesting that it served as a source of nutrients for the effective expression and translocation of MO into the medium.
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Gassmann, Aaron J., and Ryan Keweshan. Durability of Corn Expressing Bacillus thuringiensis Insecticidal Proteins in Single and Stacked Events. Ames: Iowa State University, Digital Repository, 2010. http://dx.doi.org/10.31274/farmprogressreports-180814-217.
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Williams, David D., Charles L. Turnbough, and Jr. Surface Layer Protein EA1 is Not a Component of Bacillus anthracis Spores but is a Persistent Contaminant in Spore Preparations. Fort Belvoir, VA: Defense Technical Information Center, October 2003. http://dx.doi.org/10.21236/ada424232.
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Kloepper, Joseph W., and Ilan Chet. Endophytic Bacteria of Cotton and Sweet Corn for Providing Growth Promotion and Biological Disease Control. United States Department of Agriculture, January 1996. http://dx.doi.org/10.32747/1996.7613039.bard.
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Endophytes were isolated from 16.7% of surface-disinfested seeds and 100% of stems and roots of field-growth plants. Strains from Israel with broad-spectrum in vitro antibiosis were mainly Bacillus spp., and some were chitinolytic. Following dipping of cut cotton roots into suspensions of these strains, endophytes were detected up to 72 days later by isolation and by autoradiograms of 14C-labelled bacteria. Selected endophytes exhibited biological control potential based on significant reductions in disease severity on cotton inoculated with Rhizoctonia solani or Fusarium oxysporum f. sp. vasinfectum as well as control of Sclerotium rolfsii on bean. Neither salicylic acid nor chitinase levels increased in plants as a result of endophytic colonization, suggesting that the observed biocontrol was not accounted for by PR protein production. Some biocontrol endophytes secreted chitinolytic enzymes. Model endophytic strains inoculated into cotton stems via stem injection showed only limited movement within the stem. When introduced into stems at low concentrations, endophytes increased in population density at the injection site. After examining several experimental and semi-practical inoculation systems, seed treatment was selected as an efficient way to reintroduce most endophytes into plants.