Journal articles on the topic 'Bacillus (bacteria)'
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Субботин, A. Subbotin, Бажин, A. Bazhin, Калёнова, L. Kalenova, Новикова, and M. Novikova. "Dependence of the Biological Activity of the Permafrost Bacteria Bacillus Sp. on Temperature." Journal of New Medical Technologies 21, no. 4 (October 8, 2014): 142–48. http://dx.doi.org/10.12737/7288.
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Fossil bacteria in permafrost evolutionarily adapted to low temperatures (-5ºC), modern probiotics bacteria are adapted to living in warm-blooded animals (37ºC). It was found that at -5ºC, the enzymatic activity of fossil bacteria Bacillus sp. MG8 is a minimal strain. At lowering the incubation temperature to -16ºC, the enzymatic activity of bacte-ria MG8 increases in 3 times, at the temperature 42ºC - in a 1.5times relative IP5832 strain probiotic bacteria Bacillus cereus. Fossil strain Bacillus sp. MG8 and probiotic bacterial strain B.cereus IP5832 at incubation temperature 37ºC practically don’t differ from each other in the enzymatic activity in vitro and toxicity in laboratory animals in vivo. Incubation fossil bacteria Bacillus sp. at -5ºC allows to reduce their toxicity in warm-blooded animals in 5 times in comparison with Bacillus cereus JP5832, and to increase immunostimulating effect in the doses from 0,005•106 to 50•106 microbial cells per mouse. The obtained data show that fossil saprophytic bacteria strain MG8 Bacillus sp. from permafrost are less toxic to modern mammals than even bacilli-probiotics for medical purposes.
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Stiller, Alison, Ashley Fink, and David Mitchell. "Bacillus cereus & Bacillus pumilus Harvested from a Copper Roof Inhibit the Growth of Other Microorganisms." American Journal of Undergraduate Research 17, no. 2 (September 30, 2020): 3–11. http://dx.doi.org/10.33697/ajur.2020.016.
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Bacteria growing under the effects of unique selective pressures have distinct adaptations allowing them to survive. Copper surfaces present challenges for bacterial survival because ions dissolve from the surfaces and disrupt cell membranes, thus inhibiting bacterial growth. In this study, the copper roof of Simons Hall in Collegeville, Minnesota was sampled for bacterial species during November 2018. Bacteria were isolated and grown in culture, and zones of inhibition were identified surrounding three of the bacterial colonies. Polymerase chain reaction (PCR) was used to identify two of the bacteria samples as Bacillus cereus and a third sample as Bacillus pumilus. Bacilli are large, rod-shaped, gram-positive bacteria commonly found in diverse environments. They are endospore-forming aerobes or facultative anaerobes. Initial experiments indicated that all three Bacillus strains had the ability to inhibit the growth of three environmental microorganisms. Results from growth curve experiments depicted inhibitory effects on environmental microorganisms at all stages of the growth curve, which is contrary to the prediction that the inhibitory behavior would appear at one specific period of the growth curve. Additional experiments involved plating isolates of Bacillus cereus and Bacillus pumilus with laboratory samples of Pseudomonas aeruginosa, Streptococcus pneumoniae, and Listeria monocytogenes to further understand the effectiveness of B. cereus and B. pumilus at inhibiting the growth of other microorganisms. These findings support previous studies and suggest that Bacillus are capable of inhibiting or killing other organisms. Further research will be conducted to illuminate the inhibitory mechanisms and identify potential therapeutic possibilities. KEYWORDS: Bacteria; Copper; Resistance; Growth Curve; Inhibition; Bacillus; Bacteriocin; Antimicrobial Peptides
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Łubkowska, Beata, Joanna Jeżewska-Frąckowiak, Michał Sroczyński, Magdalena Dzitkowska-Zabielska, Aleksandra Bojarczuk, Piotr M. Skowron, and Paweł Cięszczyk. "Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements." Microorganisms 11, no. 2 (February 16, 2023): 488. http://dx.doi.org/10.3390/microorganisms11020488.
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So far, Bacillus species bacteria are being used as bacteria concentrates, supplementing cleaning preparations in order to reduce odor and expel pathogenic bacteria. Here, we discuss the potential of Bacillus species as ‘natural’ probiotics and evaluate their microbiological characteristics. An industrial microbiological concentrate CS-4 of mixed Bacillus species cultures was tested, which may be a promising bacteria source for food probiotic preparation for supplementary diet. In this study, antagonistic activities and probiotic potential of Bacillus species, derived from an industrial microbiological concentrate, were demonstrated. The cell free supernatants (CFS) from Bacillus licheniformis mostly inhibited the growth of foodborne pathogenic bacteria, such as Escherichia coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021, and Staphylococcus aureus KCCM 11335, while some of Bacillus strains showed synergistic effect with foodborne pathogenic bacteria. Moreover, Bacillus strains identified by the MALDI TOF-MS method were found sensitive to chloramphenicol, kanamycin, and rifampicin. B. licheniformis and B. cereus displayed the least sensitivity to the other tested antibiotics, such as ampicillin, ampicillin and sulfbactam, streptomycin, and oxacillin and bacitracin. Furthermore, some of the bacterial species detected extended their growth range from the mesophilic to moderately thermophilic range, up to 54 °C. Thus, their potential sensitivity to thermophilic TP-84 bacteriophage, infecting thermophilic Bacilli, was tested for the purpose of isolation a new bacterial host for engineered bionanoparticles construction. We reason that the natural environmental microflora of non-pathogenic Bacillus species, especially B. licheniformis, can become a present probiotic remedy for many contemporary issues related to gastrointestinal tract health, especially for individuals under metabolic strain or for the increasingly growing group of lactose-intolerant people.
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Annisa, Rafika, Kartika Manalu, and Rizki Amelia Nasution. "Screening of Antimicrobial Producing Bacteria from Berawe Beach Sand on Kampai Pangkalan Susu Island against Pathogenic Bacteria." Jurnal Biologi Tropis 24, no. 1 (January 11, 2024): 16–25. http://dx.doi.org/10.29303/jbt.v24i1.6334.
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Sand is naturally a place to live, grow and develop some marine animals and microorganisms, usually bacteria originating from the sea area that have a large number of bioactive compounds that can produce various kinds of secondary metabolites for further production of antimicrobials. The purpose of this study was to obtain bacteria that have the potential to produce antimicrobials and to characterize bacterial isolates on the sand of Berawe Beach, Kampai Island, Pangkalan Susu. In this study several stages were carried out, namely sampling, isolation, purification, morphological characterization, antimicrobial activity test, gram staining, biochemical test and determining the bacterial genus. The results of this study obtained 9 isolates (SP14A. SP15A, SP16A, SP24A, SP24B, SP35A, SP44A, SP44B and SP45A) which have the potential as antimicrobial producers. Characterization of antimicrobial bacterial isolates from the sand of Berawe Beach, Kampai Island, Pangkalan Susu, namely isolate SP14A, which is a gram- positive bacterium in the form of streptobacilli. SP15A, SP24B and SP44B isolates were coccus-shaped gram-positive bacteria. SP16A, SP35A, SP44A and SP45A isolates were gram-positive bacteria in the form of bacilli. And isolate SP24A is a gram-negative bacterium in the form of streptobacilli. All isolates produce catalase enzymes but do not use carbon and energy. Isolates that are motile (SP14A, SP15A, SP16A, SP24A, SP35A, SP44A and SP45A) can ferment glucose while non-motile (SP24B and SP44B) cannot ferment glucose. These bacteria come from the genera Bacillus, Micrococcus, LactoBacillus and Alcaligenes. Species of Bacillus pumilus, Bacillus subtilis, Bacillus firmus, LactoBacillus bulgaricus, Micrococcus luteus and Alcaligenes eutrophus.
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Sumardi, Sumardi, Salman Farisi, Christina Nugroho Ekowati, and Rizka Oktavia. "Uji Tantang Bakteri Bacillus Kandidat Probiotik secara Invitro terhadap Bakteri Vibrio harveyi." JURNAL BIOLOGI PAPUA 11, no. 2 (October 31, 2019): 57–63. http://dx.doi.org/10.31957/jbp.799.
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This study aims to isolate Bacillus that can fight the growth of Vibrio harveyi . Based on the results of the inter-Bacillus competition test show that Bacillus isolates was able to compete and grow with each other on the SWCA media. The challenge test Bacillus bacterial to against Vibrio harveyi bacteria, that Bacillus did not yet produce anti-bacteria on the second day. In the joint culture test method between Bacillus and Vibrio harveyi that Bacillus were able to inhibit the growth of Vibrio harveyi bacteria on the 4th day.
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Ngalimat, Mohamad Syazwan, Raja Noor Zaliha Raja Abd. Rahman, Mohd Termizi Yusof, Amir Syahir, and Suriana Sabri. "Characterisation of bacteria isolated from the stingless bee, Heterotrigona itama, honey, bee bread and propolis." PeerJ 7 (August 22, 2019): e7478. http://dx.doi.org/10.7717/peerj.7478.
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Bacteria are present in stingless bee nest products. However, detailed information on their characteristics is scarce. Thus, this study aims to investigate the characteristics of bacterial species isolated from Malaysian stingless bee, Heterotrigona itama, nest products. Honey, bee bread and propolis were collected aseptically from four geographical localities of Malaysia. Total plate count (TPC), bacterial identification, phenotypic profile and enzymatic and antibacterial activities were studied. The results indicated that the number of TPC varies from one location to another. A total of 41 different bacterial isolates from the phyla Firmicutes, Proteobacteria and Actinobacteria were identified. Bacillus species were the major bacteria found. Therein, Bacillus cereus was the most frequently isolated species followed by Bacillus aryabhattai, Bacillus oleronius, Bacillus stratosphericus, Bacillus altitudinis, Bacillus amyloliquefaciens, Bacillus nealsonii, Bacillus toyonensis, Bacillus subtilis, Bacillus safensis, Bacillus pseudomycoides, Enterobacter asburiae, Enterobacter cloacae, Pantoea dispersa and Streptomyces kunmingensis. Phenotypic profile of 15 bacterial isolates using GEN III MicroPlate™ system revealed most of the isolates as capable to utilise carbohydrates as well as amino acids and carboxylic acids and derivatives. Proteolytic, lipolytic and cellulolytic activities as determined by enzymatic assays were detected in Bacillus stratosphericus PD6, Bacillus amyloliquefaciens PD9, Bacillus subtilis BD3 and Bacillus safensis BD9. Bacillus amyloliquefaciens PD9 showed broad-spectrum of antimicrobial activity against Gram-positive and Gram-negative bacteria in vitro. The multienzymes and antimicrobial activities exhibited by the bacterial isolates from H. itama nest products could provide potential sources of enzymes and antimicrobial compounds for biotechnological applications.
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Ngalimat, Mohamad Syazwan, Radin Shafierul Radin Yahaya, Mohamad Malik Al-adil Baharudin, Syafiqah Mohd Yaminudin, Murni Karim, Siti Aqlima Ahmad, and Suriana Sabri. "A Review on the Biotechnological Applications of the Operational Group Bacillus amyloliquefaciens." Microorganisms 9, no. 3 (March 17, 2021): 614. http://dx.doi.org/10.3390/microorganisms9030614.
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Bacteria under the operational group Bacillus amyloliquefaciens (OGBa) are all Gram-positive, endospore-forming, and rod-shaped. Taxonomically, the OGBa belongs to the Bacillus subtilis species complex, family Bacillaceae, class Bacilli, and phylum Firmicutes. To date, the OGBa comprises four bacterial species: Bacillus amyloliquefaciens, Bacillus siamensis, Bacillus velezensis and Bacillus nakamurai. They are widely distributed in various niches including soil, plants, food, and water. A resurgence in genome mining has caused an increased focus on the biotechnological applications of bacterial species belonging to the OGBa. The members of OGBa are known as plant growth-promoting bacteria (PGPB) due to their abilities to fix nitrogen, solubilize phosphate, and produce siderophore and phytohormones, as well as antimicrobial compounds. Moreover, they are also reported to produce various enzymes including α-amylase, protease, lipase, cellulase, xylanase, pectinase, aminotransferase, barnase, peroxidase, and laccase. Antimicrobial compounds that able to inhibit the growth of pathogens including non-ribosomal peptides and polyketides are also produced by these bacteria. Within the OGBa, various B. velezensis strains are promising for use as probiotics for animals and fishes. Genome mining has revealed the potential applications of members of OGBa for removing organophosphorus (OPs) pesticides. Thus, this review focused on the applicability of members of OGBa as plant growth promoters, biocontrol agents, probiotics, bioremediation agents, as well as producers of commercial enzymes and antibiotics. Here, the bioformulations and commercial products available based on these bacteria are also highlighted. This review will better facilitate understandings of members of OGBa and their biotechnological applications.
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Elamary, Rokaia, and Wesam M. Salem. "Optimizing and purifying extracellular amylase from soil bacteria to inhibit clinical biofilm-forming bacteria." PeerJ 8 (November 2, 2020): e10288. http://dx.doi.org/10.7717/peerj.10288.
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Background Bacterial biofilms have become a major threat to human health. The objective of this study was to isolate amylase-producing bacteria from soil to determine the overall inhibition of certain pathogenic bacterial biofilms. Methods We used serial dilution and the streaking method to obtain a total of 75 positive amylase isolates. The starch-agar plate method was used to screen the amylolytic activities of these isolates, and we used morphological and biochemical methods to characterize the isolates. Optimal conditions for amylase production and purification using Sephadex G-200 and SDS-PAGE were monitored. We screened these isolates’ antagonistic activities and the purified amylase against pathogenic and multi-drug-resistant human bacteria using the agar disk diffusion method. Some standard antibiotics were controlled according to their degree of sensitivity. Finally, we used spectrophotometric methods to screen the antibiofilm 24 and 48 h after application of filtering and purifying enzymes in order to determine its efficacy at human pathogenic bacteria. Results The isolated Bacillus species were Bacillus megaterium (26.7%), Bacillus subtilis (16%), Bacillus cereus (13.3%), Bacillus thuringiesis (10.7%), Bacillus lentus (10.7%), Bacillus mycoides (5.3%), Bacillus alvei (5.3%), Bacillus polymyxa (4%), Bacillus circulans (4%), and Micrococcus roseus (4%). Interestingly, all isolates showed a high antagonism to target pathogens. B. alevi had the highest recorded activity (48 mm) and B. polymyxa had the lowest recorded activity (12 mm) against Staphylococcus aureus (MRSA) and Escherichia coli, respectively. On the other hand, we detected no antibacterial activity for purified amylase. The supernatant of the isolated amylase-producing bacteria and its purified amylase showed significant inhibition for biofilm: 93.7% and 78.8%, respectively. This suggests that supernatant and purified amylase may be effective for clinical and environmental biofilm control. Discussion Our results showed that soil bacterial isolates such as Bacillus sp. supernatant and its purified amylase are good antibiofilm tools that can inhibit multidrug-resistant former strains. They could be beneficial for pharmaceutical use. While purified amylase was effective as an antibiofilm, the isolated supernatant showed better results.
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Samsu Adi Rahman, Sukenda, Widanarni, Alimuddin, and Julie Ekasari. "Characterization of fermentation liquid from mangrove leaves Avicennia marina and its inhibitory potential for bacterium causing ice-ice disease." Jurnal Akuakultur Indonesia 19, no. 1 (January 24, 2020): 1–9. http://dx.doi.org/10.19027/jai.19.1.1-9.
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ABSTRACT
Fermentation liquid from mangrove leaves Avicennia marina contains microorganisms, nutrients, and secondary metabolites. This study aimed to identify bacteria and the compounds in fermentation liquid of mangrove leaves A. marina and measured their inhibitory capacity against pathogenic bacteria Stenotrophomonas maltophilia which causes ice-ice disease in seaweed. Molecular analysis which aimed the 16S rRNA gene showed that the bacteria in fermentation liquid consisted of eight types of Bacillus, Bacillus subtilis MSAR-01, Bacillus megaterium MSAR-02, Bacillus firmus MSAR-03, Bacillus thuringiensis MSAR-04, Bacillus subterranerus MSAR-05, Bacillus vietnamensis MSAR-06, Bacillus sp. MSAR-07, Bacillus circulans MSAR-08, with the best inhibitory power indicated by B. subtilis MSAR-01, B. vietnamensis MSAR-06, and Bacillus sp. MSAR-07. The administration of lactic acid, bacteriocin, total fermentation liquid, and supernatant as much as 15 mL produce inhibition to S. maltophilia indicated better result than using one or a combination of several types of bacterial isolates. The inhibition of single bacterial enriched fermentation and supernatant liquids was better than bacterial combination enrichment.
Keywords: Avicennia marina, fermentation, ice-ice, mangrove
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Qodriyah, Nur Romadhona Lailatul, Eli Hendrik Sanjaya, Roswanira Abdul Wahab, and Evi Susanti. "Exploration The Candidates of Xenobiotic Degrading Indigenous Bacteria from Probolinggo City Landfill by Using Next Generation Sequencing (NGS)." Jurnal Kimia Valensi 9, no. 2 (December 28, 2023): 280–87. http://dx.doi.org/10.15408/jkv.v9i2.34316.
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Soil bacteria from tropical environments play a significant role in resolving various environmental issues, including biodegradation. Exploratory research on biodiversity is crucial to develop and harness the potential of different types of soil bacteria that are highly abundant. The bacterial diversity in landfills is typically high due to the decomposition of organic and inorganic waste, creating a favorable medium for the growth and development of soil bacteria. This study aims to assess the candidates of xenobiotic degrading indigenous bacteria from the Probolinggo City landfill using Next Generation Sequencing (NGS) method. The research stages include: 1) sampling, 2) isolation of genomic DNA from samples using the ZymoBIOMICS DNA MiniPrep Kit from Zymo Research, 3) amplification of isolated DNA with primers 16S 27F – 1429R, 4) sequencing the results of DNA amplification with NGS, 5) downstream analysis of the results using software Pavian Krona Tools, and 6) narrative analysis review to identify the candidates of xenobiotic degrading indigenous bacteria. The results show that soil samples from the Probolinggo City landfill exhibited a high diversity of bacterial communities. Based on NGS analysis, 2400 bacterial species were identified, comprising 56 genera, 17 orders, 4 classes, and 4 phyla, with respective abundances of Proteobacteria (70%), Firmicutes (15%), Planctomycetes (2%), and Cyanobacteria (0,3%). Based on the narrative analysis review, several bacteria in the Probolinggo City landfill exhibited potential as: 1) polypropylene-degrading bacteria, including Bacillus cereus, B. licheniformis and B. thuringiensis. 2) styrofoam degrading bacteria, namely Bacillus amyloliquefaciens, Bacillus cereus, Bacillus firmus and Pseudomonas aeruginosa. 3) total ammonia nitrogen (TAN) reducing bacteria, including Bacillus megaterium. 4) pesticide degrading bacteria Profenofos and Chlorantraniliprole, including Bacillus stearothermophilus. and 5) tannic acid degrading bacteria, including Pantoea dispersa. These results indicate that the Probolinggo City landfill is a good habitat for various xenobiotic-degrading bacteria, then the isolation of specific bacteria can be designed using an appropriate selective medium.
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Shettima, Hussaini, Ibrahim Alkali Allamin, Nasir Halima, Haruna Yahya Ismail, and Yusuf Musa. "Isolation and Characterization of Hydrocarbon-degrading Bacteria in Soils of Mechanical Workshops in Maiduguri, Borno State." Journal of Environmental Bioremediation and Toxicology 4, no. 2 (December 31, 2021): 35–38. http://dx.doi.org/10.54987/jebat.v4i2.632.
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The study was conducted to determine the distribution of hydrocarbon utilizing bacteria in spent engine oil (SEO) contaminated soil. Five mechanical workshops within Maiduguri Metropolis. Five bulk soil sample comprising of one each of the five sites; the sites are Leventis Area on Kashim Ibrahim Way, Damboa Raod, Boiler (commonly called Bola) near Maiduguri Monday Market, Ngomari, on Kano Road, and 1000 Housing Estate, also on Kano Road. Nutrient agar was used, or isolation and enumeration total heterotrophic bacteria and Mineral salt agar was used for the isolation and enumeration of hydrocarbon utilizing bacteria. The result shows THB (92.0x104) at Damboa road (DR) to as higher and (78.8x104) at Bola area (BL) while HUB (2.0x104) at Damboa road (DR) and as high as (9.3x104) at Bola area (BL). The bacterial species isolated were species of Bacillus licheniformis, Bacillus subtilis, Bacillus coagulans, Bacillus alvei, Bacillus cereus, Bacillus lentus other are Pseudomonas aeruginosa, Klebsiella pneumonia. Bacillus licheniformis, and Bacillus subtilis are the most occurring bacterial isolates identified. The ability of those bacterial isolate to degrade hydrocarbon buoyantly will help in remediation of oil polluted environments.
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Purwaningsih, Desi, and Destik Wulandari. "Uji Aktivitas Antibakteri Hasil Fermentasi Bakteri Endofit Umbi Talas (Colocasia esculenta L) terhadap Bakteri Pseudomonas aeruginosa." Jurnal Sains dan Kesehatan 3, no. 5 (October 31, 2021): 750–59. http://dx.doi.org/10.25026/jsk.v3i5.622.
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Bacterial endophyte is an endosimbiont which lives within host plans without causing apparent disease on its host plans. Some endophytic bacteria found in plants have the capability to produce potential compounds as antibacterial. This study aimed to determine the antibacterial activity of the endophytic bacteria fermentation from the tubers of taro plants and determine the optimum time of fermentation which produce the highest antibacterial activity. This research was initially started by producing the suspension of the endophytic bacteria and test bacteria. The suspension that had been made then was used to characterize and identify the endophytic bacteria and test bacteria. Identification methods include colony identification, Gram staining, Spore staining and biochemical tests planted on MCH, SIM, LIA and Citrate mediums. The fermentation was then carried out to produce secondary metabolite compounds which were utilized to examine the antibacterial activity against Pseudomonas auriginosa. The antibacterial activity test was conducted by the disk diffusion assay. The results of the antibacterial activity assay showed that Bacillus siamenssis and Bacillis subtilis were both able to produce secondary metabolites that have potential as antibacterial against Pseudomonas earuginosa. The highest value was achieved by 72 hours fermentation time with a value of 8.7 by Bacillus siamensis. The endophytic bacteria Bacillus subtilis had the highest activity value at 48 hours fermentation time with an average of diameter of inhibition zone of 8.97.
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Parisa, Mohammadi, Tozlu Elif, Kotan Recep, and Kotan Merve Şenol. "Potential of some bacteria for biological control of postharvest citrus green mould caused by Penicillium digitatum." Plant Protection Science 53, No. 3 (May 28, 2017): 134–43. http://dx.doi.org/10.17221/55/2016-pps.
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Ten bacteria isolate (4 Bacillus subtilis, 2 Bacillus pumilus, 2 Bacillus cereus, 1 Bacillus megaterium, and 1 Agrobacterium radiobacter) were tested in vitro for antagonistic properties against Penicillium digitatum, the causal agent of citrus green mould. The effect of these bacteria was also observed on mycelial growth, spore germination, and spore production of the pathogenic fungus in broth culture. Extracellular enzyme activities of the bacteria were determined. According to the results of in vitro antagonistic tests and enzymes activities, the most promising bacteria were Bacillus subtilis and Agrobacterium radiobacter. These bacteria were tested for disease suppression on lemon fruits. In addition, these bacterial isolates also showed remarkable antifungal activity against the pathogen on lemon fruits. The results of this study showed that Bacillus subtilis and Agrobacterium radiobacter showed remarkable antifungal activity against the pathogen. Chitinase and glucanase enzyme activity of all the tested bacteria was positive. Protease enzyme activity was positive in all tested bacteria with the exception of Agrobacterium radiobacter. In addition, all bacteria inhibited mycelial growth and spore germination (except Agrobacterium radiobacter) of the fungus. Bacillus subtilis, Bacillus cereus, and Agrobacterium radiobacter inhibited spore production in broth culture. Bacillus subtilis and Agrobacterium radiobacter were tested on lemon fruits significantly reduced disease severity. Consequently, these isolates can be used as new biocontrol agents in controlling the post-harvest decay of citrus fruits caused by Penicillium digitatum.
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Malkova, Angelina, Ivan Evdokimov, Maxim Shirmanov, Alena Irkitova, and Dina Dudnik. "Development of a microbiological preparation for crops based on Bacillus pumilus strains." BIO Web of Conferences 36 (2021): 07012. http://dx.doi.org/10.1051/bioconf/20213607012.
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Data of the microbial biopreparation development for protection and crop growth stimulation on the Bacillus bacteria basis are presented. Three B. pumilus strains isolated from the Altai region (the Russian Federation) plants rhizosphere were selected as active components of the bacterial preparation. L-bulone was chosen as the nutrient medium for flasks cultivation of the inoculum. A molasses-based nutrient medium was used to incubate the bacilli in a 15-liter fermenter. The finished microbial preparation was obtained in dry form. The biopreparation is a powder consisting of a lyophilically dried concentrates mixture of genus Bacillus spores. Bacilli biomass were pre-mixed with a protective medium based on gelatin and sucrose. The final number of bacteria in the microbial preparation is 1.29(±0.30) ×1012 CFU/g.
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Ibraheem, Khalida Jhalil. "Antimicrobial Activity of Extracts Bacillus Species Isolated From Baghdad Soil Against Some Human Pathogenic Microorganisms." Al Mustansiriyah Journal of Pharmaceutical Sciences 16, no. 2 (December 1, 2016): 82–87. http://dx.doi.org/10.32947/ajps.v16i2.114.
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Bacillus species are the predominant soil bacteria because of their resistant endospore formation and can produce many different antimicrobial substances.
The main aim of this study was to isolate Bacillus species from soil and investigate their antimicrobial activity against some pathogenic bacteria and fungi isolated from human.
48 soil samples were collected from different region of Baghdad city (Rashidiya, Mahmudiyah, Alkraat and Aldora) during December 2015 and analyzed for the presence of Bacillus species. Bacterial isolates were identified by using different microscopical examination, cultural characteristics, biochemical tests and confirmed by VITEK 2 bacterial identification system. The antimicrobial effects of Bacillus species extracts against some pathogenic bacteria (Gram-positive, Gram-negative) and fungi were examined. The identified Bacillus species included B. polymyxa, B. cereus, B. licheniformis, B. mycoides, B. firmus and B. subtilis.
The results indicate that the bacterial isolates showed antimicrobial activity against all tested pathogenic bacteria and fungi. B. polymyxa showed best activity against most test organisms compare to other Bacillus isolates, follow by B. subtilis, B. cereus, B. licheniformis, B. firmus and B. mycoides.
This study reveals that some Bacillus species have the ability to produce antimicrobial compounds that can be used to control microbial infections in future.
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Maulidi, Risyad Rizki, Novita Helshinta Dewi Pangaribuan, Frisdayanti Br Ginting, Princessa Fath Sheridan, and Yolanda Eliza Putri Lubis. "THE EFFECTIVENESS TEST OF TURMERIC EXTRACT TOWARD BACILUS CEREUS BACTERIA WITH THE COMPARISON OF CIPROFLOXACIN." Biospecies 13, no. 1 (January 28, 2020): 15–22. http://dx.doi.org/10.22437/biospecies.v13i1.8389.
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This research aims to find out effectiveness test of turmeric rhizome in concentration of 25%, 50%, 75% and 100% toward Bacillus cereus bacterial and Ciprofloxacin the same concentration as the comparison. Bacillus cereus Bacteria is a gram-positive rod-shaped bacteria and it is dangerous for humans. This research was conducted with an experimental method with post-test only design and sampling using purposive sampling method. The effectiveness test of turmeric rhizome extract toward Bacillus cereus bacteria was carried out by diffusion using disc paper by calculating the diameter of the bacterial inhibition zone against disc paper that had been moistened with turmeric rhizome extract or Ciprofloxacin solution. The results obtained in this research were carried out with the Post Hoc test, the test was found that there were significant differences from each treatment given with a 95% confidence index. In bacteria treated with 25% ciprofloxacin toward Bacillus cereus, the effectiveness was above 50% turmeric rhizome extract and under 75% turmeric extract. Turmeric extract of 75% 10.6 mm had bacterial effect as Ciprofloxacin 25% of 10.1 mm toward the growth of Bacillus cereus bacterial.
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Nietfeld, Jerome C., Craig L. Franklin, Lela K. Riley, David H. Zeman, and Bruce T. Groff. "Colonization of the Tracheal Epithelium of Pigs by Filamentous Bacteria Resembling Cilia-Associated Respiratory Bacillus." Journal of Veterinary Diagnostic Investigation 7, no. 3 (July 1995): 338–42. http://dx.doi.org/10.1177/104063879500700307.
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Warthin Starry staining revealed filamentous bacteria colonizing the tracheal epithelium of 41 of 88 (46.6%) pigs submitted for necropsy at 2 midwestem veterinary diagnostic laboratories. The bacteria were interspersed between and oriented parallel to the cilia. In 4 of 4 colonized pig tracheas, filamentous bacteria were demonstrated by transmission electron microscopy. The bacteria were approximately the same length and diameter as cilia, and in areas of heavy colonization the bacteria outnumbered cilia. The filamentous bacteria were similar in location and morphologic characteristics to cilia-associated respiratory (CAR) bacilli of rats, mice, rabbits, and cattle. Results of immunoperoxidase staining and polymerase chain reaction analysis indicated that the pig CAR bacillus is a different bacterium than the rat CAR bacillus. Rat CAR bacillus causes chronic respiratory disease in rats and mice. The association, if any, between pig CAR bacillus and swine respiratory disease is unknown.
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Tiwari, Sachin, Shilpa Pal, Rekha Puria, Vikrant Nain, and Rajendra Prasad Pathak. "Mechanical and Microstructure Study of the Self Healing Bacterial Concrete." Materials Science Forum 969 (August 2019): 472–77. http://dx.doi.org/10.4028/www.scientific.net/msf.969.472.
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Concrete largely used for construction material, degrades with the development of cracks that becomes easy passage for entry of chemicals and harmful compounds. Self healing capability is helpful to mitigate the deterioration of the concrete structures. This research work focuses on the self healing behaviour and mechanical properties of the bioconcrete supplemented with three different bacteria namely Bacillus sphaericus, Bacillus cohnii and Bacillus megaterium. Concrete supplemented with Bacillus cohnii exhibited 35.31% increase in compressive strength compared to control mix after 28 days. Concrete supplemented with other bacteria Bacillus sphaericus and Bacillus megaterium also showed enhanced compressive strength. Interestingly, addition of bacteria aided in healing of artificially generated cracks by formation of CaCO3 minerals. Maximum amount of healing (bacterial precipitation) which could be quantified as calcite minerals present in the bacterial concrete was 11.44% with B. cohnii confirmed by the Scanning Electron Microscope (SEM) with Energy Dispersive Spectroscopy (EDS).
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Louis Mary, L. Christilda, R. Sujatha, A. J. Chozhaa, and P. Mohideen Askar Navas. "Influence of Organic Manures (Biofertilizers) on Soil Microbial Population in the Rhizosphere of Mulberry (Morus Indica L.)." International Journal of Applied Sciences and Biotechnology 3, no. 1 (March 25, 2015): 61–66. http://dx.doi.org/10.3126/ijasbt.v3i1.12137.
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The effect of different kinds of organic manures on soil microbial population and mulberry production was assessed. A field experiment wascarried out at Periyar EVR College, Tamil Nadu, India in basic soil to study the influence of organic manures on soil bacterial population andmulberry production. The 4 groups of mulberry plants of MR2 variety were biofertilized with FYM, Azospirillum, Phosphobacteria andVermicompost respectively. The biofertilizers lodged bacteria on the rhizosphere of mulberry plants. When the root microorganism areanalyzed Farm yard manure biofertilized mulberry plant root tips had Gluconacobacter diazotrophicus, Bacillus pumilus, Pseudomonas putida,Bacillus coagulans, Bacillus sonorensis, Azotobacter chrococcum; Azospirillum biofertilized mulberry plants root tips had Bacillus coaculans,Azotobactor chrococcum, Azotobactor vinelandii, Bacillus subtilis and Azospirillum brasilense. Phosphobacteria biofertilized mulberry plantroot tips had Pseudomonas putida, Bacillus stearothermophilus, Brevibacillus borslelansis and Streptomycies thermonitrificans andvermicompost biofertilized mulberry plant root tips had lodged bacterias like Bacillus megaterium, Bacillus subtilis, Gluconacobacterdiazotrophicus, Pseudomonas putida, Azotobacter chrococcum, Azotobacter vinelandi, Bacillus stearothermophilus, Brevibacillus borslelansisand Bacillus sonorensis. Microbiology work reveals luxuriant growth of bacteria in all the biofertizer treated rhizosphere in the order FYM <Azospirillum < Phosphobacteria < Vermicompost. Increased availability of NPK and other micronutrients were noticed in T4 treated plantscompared to other treatments.DOI: http://dx.doi.org/10.3126/ijasbt.v3i1.12137 Int J Appl Sci Biotechnol, Vol. 3(1): 61-66
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Feliatra, Feliatra, and Wahyudi Andrito. "Karakterisasi Molekuler Bakteri Probiotik Ikan Kerapu Bebek Berbasis Teknik 16S Ribosomal DNA." Jurnal Natur Indonesia 10, no. 1 (May 4, 2018): 13. http://dx.doi.org/10.31258/jnat.10.1.13-17.
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The aim of research was to find the molecular characteristics of probiotic bacteria in the digestive tract of grouper fish (Chromileptes altivelis) based on 16S ribosomal DNA technique. The bacteria were isolated from stomach and intestine of the fish and were analized in the Marine Microbiology Laboratory of fishery and marine Science Faculty of Riau University. The bacterial DNA were isolated by using a PCR (polymerase Chain Reaction) and was conducted in the biotechnology laboratory of Diponegoro University, Semarang. The purified DNA was sequenced in the BPPTTangerang. Result shown that 6 bacterial species may be potential as probiotic. There were Bacillus velesensis strain CR-11, Vibio alginolyticus A3G-2, Bacillus cereus site2S, uncultured bacterium clone BB3S16S-17, Bacillus subtillus strain CICC10066, and Bacillus flexus strain LF-3. these bacteria grow well at pH 2 and this indicated one of probiotic bacteria characteristics.
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Aslam, Muhammad, Faiz-Ul-Hassan Nasim, Rana Ruhi, Hassan Murad, Samina Ejaz, Muhammad Shafiq Choudhary, Ghulam Mustafa, Muhammad Ashraf, and Jameel Rehman. "Isolation and Characteristics of Biotechnologically Important Antagonistic Thermophilic Bacteria from Rhizosphere of Haloxylon salicornicum." Polish Journal of Microbiology 67, no. 1 (March 9, 2018): 49–58. http://dx.doi.org/10.5604/01.3001.0011.6142.
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Rhizobacteria are an active part of microbial population in the rhizosphere of plants. In this study, twenty rhizobacteria were isolated from the rhizosphere of a perennial grass, Haloxylon salicornicum, found in Cholistan desert, an arid landmass near Bahawalpur Pakistan, in one set of experimental conditions. Colony characteristics, biochemical and molecular analyses of these isolates were performed. All isolates were bacilli, gram positive with off-white colonies and exhibited typical bacilli colony morphology. None of the isolates was gelatinase, urease, indole, H2S and catalase producer. Eleven isolates were amylase producers and 8 isolates were acid producers. All isolates fermented glucose, 3 fermented lactose and 19 fermented fructose. Molecular data revealed that out of twenty isolates, 14 isolates showed 91–99% identity with Brevibacillus borstelensis, 4 with Bacillus subtilis (97–98%) and 2 with Bacillus licheniformis (94–99%) through BLAST analysis. All identified bacterial isolates cladded with their respective groups in the phylogenetic tree. Many (11–15 out of 20) of the isolates were more effective in inhibiting growth of the tested bacterial strains as compared to the positive control (Ampicillin 50 μg/disc). We conclude that bacilli are the predominant form populating rhizosphere of this desert grass. Among the isolated bacteria Brevibacillus borstelensis, Bacillus subtilis and Bacillus licheniformis are the most predominant species.
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Sondang, Yun, Muflihayati Muflihayati, Khazy Anty, and Ramond Siregar. "KOMPATIBILITAS BEBERAPA SPESIES Bacillus SEBAGAI BIOAKTIVATOR PUPUK ORGANIK HAYATI." Jurnal Agroteknologi 13, no. 2 (February 27, 2023): 53. http://dx.doi.org/10.24014/ja.v13i2.19526.
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Bacteria is a potential agent in biological organic fertilizer”s process. The rate of decomposition of organic matter in biological organic fertilizers process depends on the role of funtional bacteria, the type of organic matter and the microenvironment. Bacteria that are inoculated into biological organic fertilizer’s matter must be synergistic among them, so that their role as as bioactivators more efficient. A compatibility experiment among the bacteria isolates used as bioactivators have to be done in order to obtain an effective and efficient biological organic fertilizer. The objevctive of the research is to obtain information on the compatible nature of the bacterial isolates. The research was conducted at the Plant Protection Laboratory of the Payakumbuh State Agricultural Polytechnic, West Sumatra from July to August 2022. The research method began with the rejuvenation bacterial isolates of Bacillus cereus, Bacillus subtilis, Bacillus thuringiensis on NA media, then continued with a compatibility experiment using the dual culture method on NA and King’s B medium, so there were a total of 12 treatments (single and combination) with three replications. The results of the compatibility experiment on the three Bacillus bacteria did not form a halo inhibition zone and were compatible with the compatibility index value (IK) 0.67-1 on NA medium and 0.90-1.00 on King’s B medium, meaning that the growth between the three Bacillus bacteria did not inhibit each other, so that all Bacillus bacteria could be used as a bioactivator simultaneously in biological organic fertilizer’s process.
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Nseabasi-Maina N, Agwa OK, and Okpokwasili GC. "Detection of antibiotic resistance in bacteria isolated from industrial sewage." GSC Biological and Pharmaceutical Sciences 18, no. 2 (February 28, 2022): 058–65. http://dx.doi.org/10.30574/gscbps.2022.18.2.0306.
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Industrialization and indiscriminate discharge of sewage being implicated in environmental pollution is responsible for the release of micropollutants which over time is expected to alter the physiology and metabolic pathways of microbiota native to the environment. This study was undertaken to track antibiotic resistant genes in industrial wastewater. Heterotrophic bacterial count indicated a vast abundance of culturable bacteria (76-232.66× 104 Cfu/ml). Hydrocarbon utilizing bacteria count ranged between (91.66 - 111.6×104 Cfu/ml). Antibiotic resistant bacteria isolates from industrially impacted wastewater identified by Sanger’s sequencing included Bacillus lichenforminis (KF737353.1), Alkanindiges sp. 5-0-9 (LT158291.1), Bacillus thuringiensis (MK875170.1, MK517632.1 EU697392.1) Bacillus altitudinis (KY777585.1), Bacillus cereus (KR185830.1) and Bacillus subtilis (MK124647.1). This study infers that industrial effluent is a potent reservoir for antibiotic resistant bacteria of environmental and public health concerns.
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Rani, Poonam. "Data on antibiotic resistance among indoor microbiome at Meerut, India." Bioinformation 18, no. 3 (March 31, 2022): 293–96. http://dx.doi.org/10.6026/97320630018293.
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Microbial dynamics of the domestic environment and their antibiotic-resistant properties have been poorly characterized. We surveyed the microbial community and their antibiotic profiling located in the rural and urban areas of Meerut city, Uttar Pradesh, India. Results show that bacterial community load across all samples had more than 100-fold higher than fungal community (all p<0.05.). Based on population load, the kitchen of both rural (Fungal: 4.16ą1.81 vs Bacteria: 160.5ą27.13) and urban areas (Fungal: 6.2ą1.02 vs Bacteria: 205.46ą30.9) were more contaminated than living rooms (rural area-Fungal: 2.13ą0.74 vs Bacteria: 62.17ą20.68 and urban area- Fungal: 4.75ą1.68 vs Bacteria: 74.88ą7.53). Six bacteria, namely Pseudomonas sps; Citrobacter sps; Bacillus Subtilis; Brevundimonas diminuta; Bacillus megaterium; and Klebsiella pneumonia, showed dominance on all other bacterial and fungal sp hence, only these six bacteria were subjected to antibiotic sensitivity test (AST). In AST, Klebsiella pneumoniae, Bacillus Subtilis, Bacillus megaterium, were resistant to more than three antibiotics. The most sensitive strain for Ciprofloxacin, Streptomycin was Citrobacter sp. However, Pseudomonas sp was found sensitive only to Amoxillin. Brevundimonas diminuta is found most sensitive to all antibiotics. Plasmid profiling of selected bacteria suggests that antibiotic resistance properties arose from plasmids, not genomic ones. These findings give new insights into the local-scale distribution of MDR bacteria in a household environment.
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Bushra Nisar Khan, Nageen Hussain, Ayesha Aihetasham, Asia Bibi, Arshia Mukhtar, Sadaf Abdullah, and Rana Mohsin Ali. "In silico analysis of Bacillus stearothermophilus as a bioindicator for sterilizationco analysis of Bacillus stearothermophilus as a bioindicator for sterilization." Pakistan Journal of Biochemistry and Biotechnology 4, no. 2 (December 31, 2023): 59–69. http://dx.doi.org/10.52700/pjbb.v4i2.229.
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To kill every kind of viable bacterial cells, sterilization in food and pharmaceutical industries is essential. In this study, bio indicators are used to validate accurate sterility. Bacillus stearothermophilus is used as a reference standard heat stable bio indicator. A 16S ribosomal RNA ribotyping sequence of Bacillus stearothermophilus is compared with different bacterial bioindicators including Bacillus pumilus, Bacillus atrophaeus, Clostridium sporogenes, and Brevundimonas diminuta. Results showed that Bacillus pumilus has the highest percentage (90.88%) similarity with the Geobacillus stearothermophilus followed by the Bacillus atrophaeus (90.84%), and Clostridium sporogenes (85.35%). The Brevundimonas diminuta was the least similar organism having a percentage similarity of 81.71% with the Geobacillus stearothermophilus. Bacterial spores are used as biological indicators for sterilization because they exhibit resistance to chemical and physical sterilization techniques. The protein common in all bacteria under study is Spo0A that make the bacteria heat resistant. The eradication of all these kind of heat resistant spores needs a high temperature which will also kill other viable cells.
Keywords: Bacillus stearothermophilus; Bacillus pumilus; Bacillus atrophaeus; Clostridium sporogenes and Brevundimonas diminuta; Spo0A protein.
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Chukwuma, Ogechukwu Bose, Mohd Rafatullah, Riti Thapar Kapoor, Husnul Azan Tajarudin, Norli Ismail, Masoom Raza Siddiqui, and Mahboob Alam. "Isolation and Characterization of Lignocellulolytic Bacteria from Municipal Solid Waste Landfill for Identification of Potential Hydrolytic Enzyme." Fermentation 9, no. 3 (March 18, 2023): 298. http://dx.doi.org/10.3390/fermentation9030298.
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The utilization of lignocellulose biomass as an alternative source of renewable energy production via green technology is becoming important, and is in line with sustainable development goal initiatives. Lignocellulolytic bacteria, such as Bacillus spp., can break down biomass by producing hydrolytic enzymes, which are crucial in the successful conversion of biomass or lignocellulosic material into renewable energy. This information gave rise to this study, where municipal solid waste sediments of a sanitary municipal solid waste landfill were sampled and screened, and lignocellulolytic bacteria were isolated and characterized. Samples were taken from four different locations at the Pulau Burung landfill site in Malaysia. Lignin and starch were used as sources of carbon to identify potential bacteria that exhibit multi-enzymatic activity. The growth rate and doubling time of bacterial isolates in lignin and starch were taken as the criteria for selection. Eleven bacterial isolates were screened for cellulase activity using iodine and Congo red dyes. The cellulase activity of these isolates ranged from 0.8 to 1.7 U/mL. We carried out 16S rRNA gene sequencing to identify the phyla of the selected bacterial isolates. Phylogenetic analysis was also conducted based on the 16S rRNA sequences of the bacterial isolates and related Bacillus species, and a tree was generated using the Neighbor-Joining method. In this study, Bacillus proteolyticus, Bacillus Sanguinis, Bacillus spizizenii, Bacillus paramycoides, Bacillus paranthracis and Neobacillus fumarioli were identified as promising bacteria capable of expressing lignocellulolytic enzymes and degrading the lignocellulosic biomass present in municipal solid waste.
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Hing, Jan Nie, Bor Chyan Jong, Pauline Woan Ying Liew, Rashid Elly Ellyna, and Shuhaimi Shamsudin. "Gamma Radiation Dose-Response of Gram-Positive and Gram-Negative Bacteria." Malaysian Applied Biology 51, no. 5 (December 26, 2022): 107–12. http://dx.doi.org/10.55230/mabjournal.v51i5.2370.
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Bacterial mutagenesis induced through gamma irradiation is one of the techniques for strain improvement. The DNA changes caused by radiation and reactive oxygen species created from water radiolysis induced bacterial mutagenesis. There is always a constant demand for better quality strains from the bioprocessing industries to speed up production and increase yield. Bacillus strains are Gram-positive bacteria whereas Escherichia coli is a Gram-negative bacteria; they are all model organisms used by the bioprocessing industries. This study investigates the effect of acute gamma irradiation on Gram-positive Bacillus megaterium NMBCC50018, Bacillus subtilis NMBCC50025 and Gram-negative Escherichia coli. Samples were irradiated in Gamma Cell Acute Irradiation Facility at Malaysian Nuclear Agency with irradiation doses from 0.1 kGy to 2.1 kGy. The radiation sources were from two Cesium-137 sealed sources. Dose responses are crucial information for bacterial mutagenesis studies. The survival curves of viable bacterial cell count versus radiation doses were plotted to determine dose-response and lethal dose, 50% (LD50). Viable cells reduce as irradiation doses increase. The LD50 for Bacillus megaterium NMBCC50018, Bacillus subtilis NMBCC50025 and Escherichia coli were 1.2 kGy, 0.2 kGy, and 0.03 kGy, respectively. Bacillus megaterium NMBCC50018 was most resistant to gamma radiation. Dose responses between Gram-positive and Gram-negative bacteria were concluded to be different.
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Burovinskaya, Margarita, Lyubov Maslienko, and Eugeniya Yurchenko. "Antimycotic activity of bacterial strains against the pathogen of grape necrotic leaf spotting Alternaria sp." E3S Web of Conferences 254 (2021): 05004. http://dx.doi.org/10.1051/e3sconf/202125405004.
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Species of the genera Bacillus Cohn and Pseudomonas Migula are successfully used as biocontrol agents for many mycopathogens, including Alternaria sp. To assess the bacteria antagonistic potential against Alternaria sp, we used the method of counter cultures on various nutrient media – universal and specialized. Of the 24 bacterial strains of the genus Bacillus, 18 strains were developed antagonistic activity to Alternaria sp. after 10 days of co-cultivation on the Tylona-3 medium. The maximum sterile zone (8.0 mm) was formed by bacterial strains (R-9, 5B-1, 01 cor f Bacillus sp.). 3 strains of 9 bacteria of the genus Pseudomonas, developed antagonistic activity to Alternaria sp. on Kinga B medium - 14-3 Pseudomonas sp., Oif 2-1 Pseudomonas sp., 14-4 Pseudomonas sp. The maximum antibiotic activity on potato-sucrose agar was established in strains 3-3 Bacillus sp., and K 1-1 Bacillus sp. (sterile zone 7.5 mm). All the tested bacteria most often caused one sign of antagonism – antibiosis.
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Chouaia, Bessem, and Jessica Dittmer. "A 2000-Year-Old Bacillus stercoris Strain Sheds Light on the Evolution of Cyclic Antimicrobial Lipopeptide Synthesis." Microorganisms 12, no. 2 (February 6, 2024): 338. http://dx.doi.org/10.3390/microorganisms12020338.
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Some bacteria (notably the genera Bacillus and Clostridium) have the capacity to form endospores that can survive for millions of years in isolated habitats. The genomes of such ancient bacteria provide unique opportunities to understand bacterial evolution and metabolic capabilities over longer time scales. Herein, we sequenced the genome of a 2000-year-old bacterial strain (Mal05) isolated from intact apple seeds recovered during archaeological excavations of a Roman villa in Italy. Phylogenomic analyses revealed that this strain belongs to the species Bacillus stercoris and that it is placed in an early-branching position compared to most other strains of this species. Similar to other Bacillus species, B. stercoris Mal05 had been previously shown to possess antifungal activity. Its genome encodes all the genes necessary for the biosynthesis of fengycin and surfactin, two cyclic lipopeptides known to play a role in the competition of Bacilli with other microorganisms due to their antimicrobial activity. Comparative genomics and analyses of selective pressure demonstrate that these genes are present in all sequenced B. stercoris strains, despite the fact that they are not under strong purifying selection. Hence, these genes may not be essential for the fitness of these bacteria, but they can still provide a competitive advantage against other microorganisms present in the same environment.
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Wihayyu, Afri, Zurai Resti, Eri Sulyanti, Darnetty Darnetty, and Ujang Khairul. "Antagonistic Test of Endophytic Bacteria Against Fusarium oxysporum f.sp. cepae Causes of Moler Disease on Shallots." CROPSAVER - Journal of Plant Protection 7, no. 1 (July 3, 2024): 35–42. http://dx.doi.org/10.24198/cropsaver.v7i1.54351.
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Fusarium oxysporum f.sp. cepae (FOCe) is a pathogenic fungus causing moler disease in shallots. One method of controlling this disease is by using endophytic bacteria. Endophytic bacteria are bacteria that live in plant tissues without causing disease symptoms. This research aims to obtain the most effective endophytic bacteria in inhibiting the growth of the pathogenic fungus FOCe. The study employs a Completely Randomized Design (CRD) consisting of 8 treatments with 3 replications each. The treatments include Bacillus sp. HI, Bacillus sp. SJI, Bacillus cereus P14, Bacillus cereus Se07, Bacillus subtilis, Serratia marcescens JB1E3, Serratia marcescens ULG1E4, and control. Tests conducted include the antibiosis test of endophytic bacterial cells using the dual culture method and the secondary metabolite test using media poisoning method. The observed parameters include inhibition zone, effectiveness of secondary metabolites, fresh weight, and dry weight of the FOCe fungus. All endophytic bacteria were capable of inhibiting the growth of FOCe. The most effective endophytic bacteria in inhibiting the growth of FOCe were found to be Serratia marcescens ULG1E4 and Serratia marcescens JB1E3. In the antibiosis test of endophytic bacterial cells, Serratia marcescens ULG1E4 exhibited an inhibition zone of 64.44%, while Serratia marcescens JB1E3 showed an inhibition zone of 61.11%. In the secondary metabolite compound test, the effectiveness values for Serratia marcescens ULG1E4 and Serratia marcescens JB1E3 were 95.31% and 95.03%, respectively.
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Zamba, Duresa Dubale, Tesfaye Alemu Mohammed, Yiglet Mebrat Getu, Dereje Hailu Tufa, and Belachew Asteray Demiss. "Experimental Investigation on Self-Healing Efficiency of Mortar with Bacillus subtilis and Bacillus cereus." Advances in Materials Science and Engineering 2023 (April 15, 2023): 1–11. http://dx.doi.org/10.1155/2023/9399101.
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With the development of cracks on their surfaces, mortar’s service life dramatically shortens. Self-healing concrete by Microbiologically Induced Calcite Precipitation (MICP) is one of the high-tech concretes being used to address these issues. This type of mortar can start biological processes to repair itself and deal with its cracks. The self-healing effectiveness of two different bacteria, in this paper, Bacillus subtilis and Bacillus cereus, added to the mortar is examined experimentally. In order to conduct this investigation, artificial cracks were made in the mortar. A 3D optical microscope was used to take repeated pictures of the cracked mortar. The mechanical and durability tests conducted on the bacterial mortar were used to gauge the efficacy of self-healing. Mortar samples were left for 7, 14, and 28 days to cure. Compressive strength, flexural strength, water absorption, and sorptivity were measured during various times of the curing process. The test results showed that the mortar with bacteria had an increase in strength and durability compared to the control mix. In the sample of mortar containing bacteria, Bacillus subtilis and Bacillus cereus a maximum increase of 17.29% and 11.31% in flexural strength, 17.77% and 12.84% in compressive strength were observed and a 34.48% and 26.43% decrease in water absorption in the mortar sample containing bacteria, Bacillus subtilis and Bacillus cereus at 28 days, respectively. The results of the mortar absorption test showed that the addition of bacteria to the mortar matrix significantly reduced the primary and secondary absorption rates of bacterial mortars B-M-1 and B-M-2. Using a 3D light microscope, the cracks in the bacterial mortar showed that larger amounts of white crystal precipitates were generated that nearly filled the surface of the crack. Overall, Bacillus subtilis appeared to be superior to Bacillus cereus based on the results of mechanical and mortar durability tests because calcium carbonate precipitates more rapidly.
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Jasime, Shaima Fakhry, Khalid Falih Hassan, and Waleed Husain Salman. "Biodegradation of organic pollutants in oil industry waste water by Bacillus Bacteria." Journal of Biotechnology Research Center 7, no. 1 (January 1, 2013): 44–49. http://dx.doi.org/10.24126/jobrc.2013.7.1.243.
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Bacillus apiarus, Bacillus filicolonicus and Bacillus laterosporus were used to degrade organic pollutants from final discharge of Al Dura wastewater treatment plant in Baghdad and reduce their concentrations by measuring Chemical Oxygen Demand (COD), Total Organic Carbon (TOC), Total Nitrogen (TN) and Total Phosphorus (TP) in laboratories conditions of 25o c, pH 5.8-6.8 in batch culture system and 18hr period treatment, the bacteria Bacillus apiarus, Bacillus filicolonicus and Bacillus laterosporus showed ability to reduce COD concentration by(82, 88.5, 94)% and TOC concentration by (73, 82 ,84)% and TN concentration reduced by (86, 91, 81)% in 18 hr period treatment with highly significant differences P<0.01 for all bacterial treatments, the bacteria in the current study showed high ability to reduce TP concentration by 100% with highly significant differences P<0.01 for all bacterial treatments in 18hr period treatment.
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Sharma, Vaidik, and Prof Mayur Singi. "Experimental Study on Bacterial based Self-Healing Concrete." International Journal for Research in Applied Science and Engineering Technology 11, no. 7 (July 31, 2023): 40–46. http://dx.doi.org/10.22214/ijraset.2023.54587.
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Abstract: The potential of concrete to self-heal has been studied in several works, hence in this study we are analyzing the strength properties of self-healing bacterial concrete by mixing bacteria (Bacillus Subtilis) with calcium source. In the study, bacteria (Bacillus Subtilis) were introduced at 3% and 5%, while calcium lactate was added at 5% and 10%, respectively. As a result, the study addresses economic issues not just for building but also for upkeep. The purpose of this experiment was to look into the strength qualities of bacterial self-healing concrete with varied ratios of Bacillus subtilis and calcium lactate. The researchers prepared concrete specimens with varied ratios of bacterial solution and calcium lactate and tested their compressive and flexural strength. The results revealed that adding Bacillus subtilis and calcium lactate to the concrete enhanced its compressive and flexural strength, with the maximum strength values recorded at a 3% bacterial solution and 5% calcium lactate combination. Furthermore, the concrete's self-healing potential was assessed by putting the samples to crack-healing experiments. The results demonstrated that the bacterial self-healing concrete containing Bacillus subtilis and calcium lactate performed better than the control samples without bacteria. The study reveals that using Bacillus subtilis and calcium lactate in concrete might increase the material's strength and self-healing capabilities, leading to enhanced durability and sustainability of concrete buildings.
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Shtenikov, M. D., O. Y. Zinchenko, and V. V. Boldyreva. "ANTAGONISTIC ACTIVITY OF MARINE BACTERIA OF BACILLUS, PRIESTIA AND PAENIBACILLUS GENERA OF DIFFERENT THERMOTYPES." Microbiology&Biotechnology, no. 2(55) (September 20, 2022): 50–65. http://dx.doi.org/10.18524/2307-4663.2022.2(55).261778.
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SummaryAim. To study the antagonistic activity of bacteria of genera Bacillus, Priestia and Paenibacillus at different conditions of cultivation. Methods. Antagonistically active spore-forming bacteria isolated from deep-sea bottom sediments of the Black Sea were used for the study. Determination of thermotypes was performed using the results of analysis of fatty acid profile parameters. Antagonistic activity to test strains of bacterial opportunistic pathogens was detected by the method of agar blocks on Gauze № 1 and Nutrient Agar media at different cultivation temperatures. Results. Aerobic bacilli of genera Bacillus, Priestia and Paenibacillus of all three thermotypes – thermotolerants, mesophiles and psychrotrophes, in general show lower antagonistic activity when cultured at 37 °C on both media, except for a marked increase in psychrotrophe antagonistic activity at 37 °С on Gauze № 1. Conclusions. It was established that belonging to a certain thermotype affects the character of the antagonistic activity of sporeforming bacteria. The antagonistic activity of mesophilic and thermotolerant bacteria at a higher temperature of cultivation is lower, and that of psychrotrophic bacteria at a higher temperature and on Gauze № 1 medium is higher.
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Bui Thi Cam Tu, Nguyen Phan Khanh An, Ho Thi Thu Ngan, Dang Thi Ngoc Thanh, and Hoang Minh Tam. "Isolation and identification of bacteria with capability of starch, cellulose and protein decomposition from Frass of Black Soldier Fly (Hermetia Illucens) Larvae." World Journal of Advanced Research and Reviews 17, no. 3 (March 30, 2023): 864–75. http://dx.doi.org/10.30574/wjarr.2023.17.3.0487.
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This research isolated and identified bacteria with capability of starch, cellulose and protein decomposition from frass of black soldier fly (Hermetia Illucens) larvae (BFL). Dilute suspension of BFL frass was spread on petri dishes with solid Nutrient Agar for bacteria isolation. Selection and evaluation of bacteria with capability of starch, cellulose and protein decomposition was based on their ability of creating halo zone on starch agar, CMC and skim milk media. Identification of selected bacterial isolates was based on 16S rDNA sequences. There were 44 bacterial strains isolated from BSFL frass. Bacterial strains with the best capability of starch, cellulose and protein decomposition were CCRL21 and CCRL16 with halo zone radii of 1.5 cm and 0.8 cm; CCRL16, CCRL20 with halo zone radii with the similar size of 0.83 cm; and CCRL44 has the best ability of protein decomposition with an average halo zone radius about 2.47 cm, respectively. Six bacterial strains with good ability of degrading cellulose, starch and protein were identified as Bacillus paramycoides CCRL1; Bacillus altitudinis CCRL16, Bacillus spizizenii CCRL20, Bacillus altitudinis CCRL21, Lysinibacillus pakistanensis CCRL32 and Paenibacillus silvae CCRL43.
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YANTI, YULMIRA, WARNITA WARNITA, REFLIN REFLIN, and MUNZIR BUSNIAH. "Indigenous endophyte bacteria ability to control Ralstonia and Fusarium wilt disease on chili pepper." Biodiversitas Journal of Biological Diversity 19, no. 4 (July 1, 2018): 1532–38. http://dx.doi.org/10.13057/biodiv/d190446.
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Yanti Y, Warnita, Reflin, Busniah M. 2018. Indigenous endophyte bacteria ability to control Ralstonia and Fusarium wiltdisease on chili pepper. Biodiversitas 19: 1532-1538. Bacterial wilt and Fusarium wilt caused by Ralstonia syzigii subsp. indonesiensisand Fusarium oxysporum f.sp. capsici (Foc), respectively are the most damaging vascular pathogens in chili pepper (Capsicum annuumL.) and many other crops limiting their production, worldwide. Various strategies have been developed to control wilt pathogensincluding the application of chemical pesticides, which generally considered as the most effective and fastest strategy for plant diseasemanagement. However, effective chemicals for wilt pathogens of chili pepper plants are not available, yet. Endophytic bacteriaconsidered as one of options to control vascular wilt disease because of its ability to live and colonize in internal roots of plants.Previous research has been done to select endophytic indigenous bacteria isolates which can promote growth rate of chili pepper. Thepurpose of the research was to identify the selected indigenous endophyte bacteria isolates acquired from our previous study using 16SrRNA identifications and to screen the selected endophytic indigenous bacteria to control both R. syzigii subsp. indonesiensis and Foc.Results from 16S r RNA analysis showed that all of 9 isolates were identified as Bacillus spp., such as Bacillus cereus ATCC 14579,Bacillus pseudomycoides strain NBRC 101232, Bacillus toyonensis strain BCT-7112, Bacillus thuringiensis strain ATCC 10792,Bacillus weihenstephanensis strain DSM 11821, Bacillus mycoides strain 273, Bacillus cereus strain NBRC 15305, Bacillusbingmayongensis strain FJAT-13831 and Bacillus manliponensis strain BL4-6. Our results showed that most of endophytic bacteriaisolates application could control both bacterial and Fusarium wilt diseases. Six out of nine isolates can suppress R. syzigii subsp.indonesiensis without developing any symptoms and five isolates could suppress symptoms of Foc. Isolates Bacillus pseudomycoidesstrain NBRC 101232 Bacillus thuringiensis strain ATCC 10792 and Bacillus mycoides strain 273 were potential for control Foc and R.syzigii subsp. indonesiensis in chili pepper.
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Yuan, Zong-Sheng, Fang Liu, and Guo-Fang Zhang. "Isolation of culturable endophytic bacteria from Moso bamboo (Phyllostachys edulis) and 16S rDNA diversity analysis." Archives of Biological Sciences 67, no. 3 (2015): 1001–8. http://dx.doi.org/10.2298/abs141212063y.
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We analyzed culturable endophytic bacteria from Moso bamboo (Phyllostachys edulis) using traditional bacterial isolation and culture methods and then studied the colony characteristics and diversity with a 16S rDNA sequence analysis. We isolated 82 endophytic bacteria strains belonging to 47 species in 26 genera from the root, rhizome, stem and leaves of Moso bamboo species from populations on Wuyi Mountain, and in the Jiangle and Changting regions. There were significant differences in the composition of the culturable endophytic bacteria isolated from the different areas and from different tissues. The dominant bacteria strains from the Wuyi Mountain samples were Arthrobacter, Staphylococcus, Bacillus and Enterobacter, while the dominant bacteria from the Jiangle samples were Bacillus, Staphylococcus and Curtobacterium, and the dominant bacteria in the Changting samples were Alcaligenes, Pseudomonas, Staphylococcus and Bacillus. Our results demonstrate the abundant diversity of endophytic bacteria in Moso bamboo.
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Setiaji, Jarod, Feli Feliatra, Hilwan Yuda Teruna, Iesje Lukistyowati, Indra Suharman, Zainal Abidin Muchlisin, and Teuku Iskandar Johan. "Antibacterial activity in secondary metabolite extracts of heterotrophic bacteria against Vibrio alginolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa." F1000Research 9 (December 21, 2020): 1491. http://dx.doi.org/10.12688/f1000research.26215.1.
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Background: Disease causing bacteria such as Vibrio alginolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa present a problem for fish farming. Treatment to remove them are generally carried out using antibiotics which have side effects on fish, the environment and humans. However, the use of antibacterial compounds derived from heterotrophic bacteria serve as a good alternative for antibiotics. Therefore, this study aimed to explore antibacterial activity in the secondary metabolite extracts of heterotrophic bacteria against Vibrio alginolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa. Methods: Heterotrophic bacteria namely Bacillus sp. JS04 MT102913.1, Bacillus toyonensis JS08 MT102920.1, Bacillus cereus JS10 MT102922.1, Bacillus sp. JS11 MT102923.1, Pseudoalteromonas sp. JS19 MT102924.1, Bacillus cereus JS22 MT102926.1, and Bacillus sp. strain JS25 MT102927.1 were used in this study. The sequences of these bacteria have been deposited and are available from NCBI GenBank. Each heterotrophic bacterium was cultured on 6L nutrient broth for 8 days, and extracts produced using ethyl acetate to obtain their secondary metabolites. These extracts were tested for their phytochemical contents using FT-IR and also tested for their inhibitory property in pathogenic bacteria by agar diffusion method. Results: Phytochemical test results showed that the seven heterotrophic bacterial isolates produced terpenoid compounds. Based on the inhibitory test, the secondary metabolite extracts from Bacillus sp strain JS04 had the highest inhibitory effect on the growth of pathogenic bacteria namely, V. alginolyticus (17.5 mm), A. hydrophila (16.8 mm), and P. aeruginosa (17.3 mm). Conclusion: It was concluded that the secondary metabolite extracts of heterotrophic bacteria inhibit the growth of V. alginolyticus, A. hydrophila, and P. aeruginosa.
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Jayadi, Lukky, Sandry Kesuma, and Muhammad Hasan Wattiheluw. "Hand sanitiser activity test of eucalyptus (Eucalyptus globulus) oil extract against Bacillus subtilis and Enterococcus faecalis bacteria." Pharmacy Education 23, no. 2 (May 15, 2023): 179–83. http://dx.doi.org/10.46542/pe.2023.232.179183.
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Background: Bacillus subtillis bacteria can cause meningitis, endocarditis, and eye infections, while Enterococcus can cause meningitis in neonates and endocarditis in adults. Therefore, it is important to use hand sanitiser to avoid bacterial infections. Eucalyptus is an essential oil-producing plant that can inhibit growth and kill bacteria with its compounds, namely linalool and pinocarveol. Purpose: To make a good eucalyptus oil hand sanitiser and test its effectiveness against Bacillus subtilis and Enterococcus faecalis bacteria. Method: Making transparent hand sanitiser with a mixture of eucalyptus oil extract. Physical, chemical and antibacterial tests on Bacillus subtilis and Enterococcus faecalis. Results: The hand sanitiser preparation obtained an acidity of 7, with a specific gravity of 0.8512 g/ml, and a viscosity of 4.14 cP and the cleaning power of Bacillus subtilis and Enterococcus faecalis was 99.9%. Conclusion: Eucalyptus oil extract hand sanitiser is of good quality and effectively kills Bacillus subtilis and Enterococcus faecalis bacteria.
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Purwanti, Ipung Fitri, IDAA Warmadewanthi, Januarti Jaya Ekaputri, Hurun In, Mulyani Zahra Paramata, and Hafidya Norista Pramesti. "Bacteria Isolation of Kerosene Contaminated Asphalt Waste (Asbuton)." IOP Conference Series: Earth and Environmental Science 1307, no. 1 (February 1, 2024): 012010. http://dx.doi.org/10.1088/1755-1315/1307/1/012010.
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Abstract Kerosene is a type of hydrocarbon commonly known as paraffin oil which is usually used as a home heating fuel, lamp and asphalt solvent. The presence of kerosene in the asphalt production can potentially cause environmental pollution, one of which affects soil conditions. Some bacteria that have specific ability to degrade hydrocarbon content in soil are called hydrocarbonoclastic bacteria. This study aims to determine the proportion of the presence of bacteria that have the potential to degrade hydrocarbons, in this case kerosene, in polluted soil at the location of one of the largest asphalt manufacturing companies in Indonesia and to determine the morphological characters of bacterial isolates. Based on the results of the study, Hydrocarbonoclastic bacteria isolated from asbuton had 5 dominant isolates and were identified by PCR analysis. The results of this study were that the five bacterial isolates were Bacillus sp. with 3 of them being Bacillus subtilis and 2 others being Bacillus cereus. This result has an Entiren stiffness of 99.62% - 100%. The type of bacteria Bacillus sp. are capable of reducing the Total Petroleum Hydrocarbon (TPH) and still able to survive and potentially degrade the TPH content in it.
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Wulandari, Nova, Mokhamad Irfan, and Robbana Saragih. "ISOLASI DAN KARAKTERISASI PLANT GROWTH PROMOTING RHIZOBACTERIA DARI RIZOSFER KEBUN KARET RAKYAT." DINAMIKA PERTANIAN 35, no. 3 (January 28, 2020): 57–64. http://dx.doi.org/10.25299/dp.2019.vol35(3).4565.
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ABSTRACT
The diversity of vegetation that grows on the ground will affect the number and type of microbes in the rhizosphere of folk rubber plantations. The purpose of this research is to know the population, genus, and biological activity of PGPR bacteria (IAA hormone producers, phosphate solvents and biocontrol agents) originating from the rhizosphere of folk rubber plantations. This research was conducted from March to May 2018 at the Laboratory of Pathology, Entomology and Microbiology Faculty of Agriculture and Animal Sciences, Islamic State University Sultan Syarif Kasim Riau and UPT Health and Environment Laboratory. This research used an observation method by taking soil samples that are composted, and data are presented in descriptive form. The parameters observed in this research were soil pH, bacterial population, characterization of PGPR bacteria includes macroscopic, microscopic, biochemical reaction test and PGPR bacterial biological activity (IAA test qualitatively, phosphate solvent test and in-vitro inhibitory test). The results showed that the pH of the soil obtained at a depth of 0-20 cm was 3.19 with a bacterial population of 1.06 × 106 CFU/g of soil. A total of 4 isolates were able to produce IAA hormones namely genus Bacillus sp.1, Bacillus sp.2, Bacillus sp.3, and Bacillus sp.5. A total of 2 isolates were able to dissolve foster, namely genus Bacillus sp.1 and Bacillus sp.2 and 2 isolates were able to play a role as inhibitory power against Fusarium sp. namely the genus Bacillus sp. 4 and Bacillus sp.5. There were 5 isolates of PGPR bacteria that had different biological activity abilities, namely genus Bacillus sp.1, Bacillus sp.2, Bacillus sp.3, Bacillus sp.4, and Bacillus sp.5.
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Sepriana, Citra, and Eti Sumiati. "Identifikasi Dan Uji Daya Hambat Isolat Bakteri Endofit Bunga Tanaman Cengkeh (Syzygium aromaticum L.) Terhadap Bakteri Patogen." Jurnal Penelitian Pendidikan IPA 6, no. 1 (January 29, 2020): 101. http://dx.doi.org/10.29303/jppipa.v6i1.340.
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This research was conducted to find out the capabilities of endophytic bacteria isolated from flowers of the clove plants in inhibiting the growth of bacteria Streptococcus mutans, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli and to identify endophytic bacteria that potensial to produce an antibacterial. Stages of this research include the isolation of endophytic bacteria from flowers of the clove plants, antibacterial test, and molecular identification based on 16S rRNA. Isolates of endophytic bacterial of clove plants flower produce 5 isolates, 4 isolate inhibited the bacteria S. aureus. Based on 16S rRNA molecular identification, endophytic bacterial isolates of clove plants flower which have inhibitory closely related to Bacillus amyloliquefasiens, Staphylococcus epidermidis 1034 MPA and Bacillus cereus JL.
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Suldina, Е. V., N. А. Feoktistova, and I. I. Bogdanov. "ISOLATION OF NEW STRAINS OF BACILLUS MEGATERIUM BACTERIA AND STUDY OF THEIR BIOLOGICAL PROPERTIES." Vestnik of Ulyanovsk state agricultural academy, no. 3(50) (September 8, 2020): 60–67. http://dx.doi.org/10.18286/1816-4501-2020-3-60-67.
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Bacillus megaterium is a gram - positive rod-shaped endospore-forming bacteria. They are used as an effective soil modifier, having the ability to disperse phosphorus. In turn, phosphorus plays an important role in several physiological and biochemical processes of plants, such as photosynthesis, the conversion of sugar to starch, and the transfer of genetic traits. Based on the current state of research in the development of biofertilizers, it can be argued that the biocomposition of Bacillus megaterium bacteria will lead to an increase in the availability of a number of macronutrients for plants – the main elements of nutrition. This article presents the results of screening studies of previously created collection of bacterial strains of Bacillus megaterium from the Museum of the Department of microbiology, virology, epizootology and VSE of Ulyanovsk SAU. The developed scheme of isolation and identification of Bacillus megaterium bacteria is given. The species identity of 6 strains of B. megaterium was confirmed. The results of the research are compared with the characteristic phenotypic properties of the studied species described in "Bergey's Manual of Systematic Bacteriology" (2015). 210 soil samples were taken to isolate new strains of microorganisms. Initial identification allowed us to isolate 58 strains of bacteria of the genus Bacillus, 16 were assigned to Bacillus megaterium. The percentage of contamination of soil samples of this type of bacteria was 7.6 %. As the result of research, the collection of bacterial strains of studied microorganisms was expanded and their taxonomic affiliation was determined. The resulting collection will be the object of research at the following stages of work on the development of biocomposition based on bacteria to increase the coefficient of absorption of mineral components of fertilizers.
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Diabankana, Roderik Zhil' Klare, and Radik Safin. "EVALUATION OF THE INFLUENCE OF THE USE OF BIOLOGICAL PREPARATIONS DURING THE VEGETATION PERIOD ON THE MICROBIOME OF SPRING WHEAT SEEDS." Agrobiotechnologies and digital farming 2, no. 1 (April 2, 2023): 22–26. http://dx.doi.org/10.12737/2782-490x-2023-22-26.
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An assessment is made of the effect of spraying plants with biological products on the total microbiome of seeds of a new crop. The studies were carried out on three varieties of spring wheat of domestic breeding - Yoldyz, Burlak and Ulyanovsk-105. Preparations based on strains of endophytic bacteria isolated from seeds of spring wheat (Bacillus mojavensis PS17, Bacillus velezensis KS25) and spring barley (Bacillus velezensis KS31, Bacillus subtilis KS38) were used as biological preparations. The treatment of spring wheat crops was carried out in the phase of entry into the tube using the biological product consumption rate of 1.0 l/ha. The impact of treatments on the fungal and bacterial microbiome was assessed using quantitative PCR methods with the determination of the total (total) DNA of microorganisms per unit weight of seeds. In most cases, the use of treatment with endophytic bacteria reduces the amount of total micromycete DNA on seeds. Among the studied isolates, the Bacillus subtilis KS-38 strain was especially distinguished, which provided a significant (7.7-11.1 times) decrease in this indicator in all varieties. Bacillus mojavensis PS-17 had a significant effect (a decrease of almost 47 times) on the Ulyanovska 105 variety, and Bacillus velezensis KS-31 on the Burlak variety. On varieties Yoldyz and Ulyanovska 105, the most significant decrease in the total DNA of micromycetes was noted for endophytes obtained from spring wheat seeds (Bacillus mojavensis PS-17, Bacillus velezensis KS-25), and on variety Burlak, endophytes from spring barley seeds (Bacillus velezensis KS-31 and Bacillus subtilis KS-38). The decrease in the total DNA of micromycetes in the experiments was due to the use of the studied biological products and, to a lesser extent, varietal characteristics. For the bacterial microbiome, there were strong differences between varieties. A significant increase in the total DNA of bacteria in all studied varieties was in Bacillus mojavensis PS-17 and Bacillus velezensis KS-31. The variety (29.9%) made the greatest contribution to the variability in the content of total bacterial DNA in seeds, and the contribution of biological preparations was lower (25.5%). In the experiments, no correlation was found between the indicators of the total DNA of micromycetes and bacteria in spring wheat seeds.
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Allamin, Ibrahim Alkali, Hussaini Shettima, Hafsat Muhammad Abdullahi, Usman Ali Bukar, Amina Umar Faruk, and Abdulhamid Musa Ruwa. "Distribution of Heterotrophic and Nitrogen fixing Rhizobacteria in Irrigation Sites of Lake Alau Borno State Northeastern Nigeria." Journal of Environmental Bioremediation and Toxicology 4, no. 2 (December 31, 2021): 27–30. http://dx.doi.org/10.54987/jebat.v4i2.630.
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This study was conducted to know the population of rhizobacteria in both irrigation and non-irrigation sites of the dam. The dense population of these organism indirectly promote plant growth and development. Five sites (A, B, C, D and E) were used to collect soil samples randomly and transported to the laboratory for analysis. Total heterotrophic bacterial count was done using nutrient agar (NA) and nitrogen fixing bacteria was counted using Ashbey’s media (AM). The result shows that highest number of total heterotrophic bacteria in site C (46.0×106) cfu/g in irrigation site whereas higher count in non-irrigation site was (13.0×106) site D, the nitrogen fixing bacterial count in irrigation site was higher at site E with (12.0×106) and for the non-irrigation site was higher at site D with (14.0×106) The total heterotrophic bacteria isolated in the soil sample are the species of Bacillus alvei, Bacillus alvei, Bacillus cereus, Bacillus cereus, Pseudomonas putida, Klebsiella aeruginosa and Enterobacter aeruginosa. Likewise, the Nitrogen fixing bacteria isolated are the species of Rhizobium leguminosarum, Klebsiella pneumonia, Bacillus lentus, Azotobacter nigricans, Azotobacter tropicalis, Azotobacter spp, and Azotobacter tropicalis. The long history of agricultural activities in the lake area has directly influenced the diversity of microbial population in the area.
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Roi, A. O., O. V. Matseliukh, P. D. Zubko, L. D. Varbanets, and I. K. Kurdysh. "PROTEOLYTIC ACTIVITY OF PHOSPHOROUS MOBILIZING BACTERIA OF BACILLUS GENUS AND THEIR INFLUENCE ON SOME PHYTOPHAGES." Agriciltural microbiology 20 (December 22, 2014): 66–73. http://dx.doi.org/10.35868/1997-3004.20.66-73.
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The research results have revealed the proteolytic activity of phosphate-mobilizing sporeforming bacteria of Bacillus genus which indices were even higher at bacteria cultivation on the gelatin media. Bacillus megaterium 2 had exhibited the highest activity among other 16 bacilli strains. Treatment of floral plants (coleus, pelargonium) planted in greenhouse, with the suspension of bacilli strains with different proteolytic activity had considerably decreased the quantity of greenhouse whitefly (Trialeurodes voparariorum) and green peach aphid (Aulacorthum circumflexsus) phytophages by 50–70 %. It was shown that subsequent treatment of the plants with suspensions of the studied bacteria is an effective biocontrol mean of phytophages quantity in greenhouse conditions.
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Resti, Zurai, Trimurti Habazar, Deddi Prima Putra, and Nasrun . "CHARACTERIZATION OF ENDOPHYTIC BACILLUS ISOLATED FROM SHALLOT ROOT AS BIOCONTROL OF BACTERIAL LEAF BLIGHT DISEASE." JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA 18, no. 1 (August 2, 2018): 31. http://dx.doi.org/10.23960/j.hptt.11831-38.
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Characterization of Endophytic Bacillus Isolated from Shallot Root as Biocontrol of Bacterial Leaf Blight Disease. Endophytic Bacillus isolated from the roots of healthy shallots has potential as biological control agent of bacterial leaf blight (BLB) disease. Based on the in planta screening, four endophytic bacteria that capable of controlling BLB diseases from the group of Bacillus were obtained, such as Bacillus cereus P14, Bacillus cereus Se07, Bacillus sp. HI, and Bacillus sp. SJI. The aims of this study were to investigate the characteristics of endophytic Bacillus that capable of controlling BLB disease and to determine the characteristic that most responsible in the disease control. This study method was descriptive. The characteristics observed were production of antibiotics by paper disc method, production of salicylic acid by capillary electrophoresis, and root colonization by in planta method. Linear regression analysis was used to determine the characteristic most responsible in the disease control. The results showed that four endophytic Bacillus were capable of producing salicylic acid and colonizing the root, and three of them were also capable of producing antibiotic. Salicylic acid production varied ranging from 13.96 to 14.72 ppm mL-1. Three endophytic Bacillus were capable of producing antibiotic with inhibition zone of 16.25 to 20.25 mm. Endophytic Bacillus was able to colonize the shallot roots with a population of bacteria ranged from 3.20 × 105-6, 20 × 105 CFU g-1 root. Based on the correlation coefficient of linear regression analysis, the root colonization of endophytic bacteria played the role in decreasing BLB disease.
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Wagi, Shabana, and Ambreen Ahmed. "Bacillus spp.: potent microfactories of bacterial IAA." PeerJ 7 (July 23, 2019): e7258. http://dx.doi.org/10.7717/peerj.7258.
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Background Auxin production by bacteria is one of the most important direct mechanisms utilized by plant growth-promoting bacteria (PGPB) for the betterment of plants naturally because auxin is a plant friendly secondary metabolite synthesized naturally by bacteria, and hence improves the growth of associated plants. So, the current study focuses on bacterial synthesis of Indole-3-acetic acid (IAA) for plant growth improvement. Methods In the current study, the PGPB were selected on the basis of their auxin production potential and their growth promoting attributes were evaluated. Indole-3-acetic acid producing potential of two selected bacterial isolates was observed by varying different growth conditions i.e., media composition, carbon sources (glucose, sucrose and lactose) and different concentrations of precursor. Influence of various physiological factors (temperature and incubation time period) on IAA production potential was also evaluated. Results Both the bacterial strains Bacillus cereus (So3II) and B. subtilis (Mt3b) showed variable potential for the production of bacterial IAA under different set of growth and environmental conditions. Hence, the IAA production potential of the bacterial isolates can be enhanced by affecting optimum growth conditions for bacterial isolates and can be used for the optimal production of bacterial IAA and its utilization for plant growth improvement can lead to better yield in an eco-friendly manner.
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Pașca, Claudia, Liviu Alexandru Mărghitaș, Ioana Adriana Matei, Victorița Bonta, Rodica Mărgăoan, Florina Copaciu, Otilia Bobiș, Maria Graça Campos, and Daniel Severus Dezmirean. "Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation." Applied Sciences 11, no. 13 (June 23, 2021): 5816. http://dx.doi.org/10.3390/app11135816.
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This study aimed to characterize raw honeys from different geographical origins in Romania, in respect of chemical composition, microbiological examination and evaluate their probiotic potential. The physico-chemical determinations were performed in APHIS-DIA Laboratory, Cluj-Napoca, Romania, using standard validated methods. Bacterial identification was performed for each sample and each colony type using Vitek® 2 Compact 15 system and PCR amplification using 16S rDNA bacterial universal primers (27F, 1492R), species being confirm by sequences analysis. In five raw honey samples, we have identified probiotic bacteria, such as: Bacillus mycoides, Bacillus thuringiensis, Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus velezensis. Generally, all honey samples meet the standard values for chemical composition. However, one sample having 7.44% sucrose was found to have also probiotics bacteria from the genus Bacillus because sucrose is a substrate for probiotics development. In conclusion, the Romanian raw honey can be a potential reservoir of probiotics, which confer a health benefit for consumers.
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Angio, Nur'ain Marton, Aryati Abdul, Syam S. Kumaji, Wirnangsi Din Uno, Yuliana Retnowati, and Magfirahtul Jannah. "Analysis of Amylase Activity in Bacteria Isolated from Hot Spring of Pentadio Resort." JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS 10, no. 1 (March 31, 2024): 266–75. http://dx.doi.org/10.36987/jpbn.v10i1.5506.
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The hot spring of Pentadio Resort is one of the geothermal areas likely to be the place of bacteria or microorganisms that can produce amylolytic enzymes. This research aims to analyze bacteria in the Pentadio Resort hot springs that possess the potential to produce amylase enzymes, analyze the characteristics of bacterial isolates that produce amylase enzymes in Pentadio Resort hot springs and determine the types of bacteria that produce amylase enzymes in Pentadio Resort hot springs. The research employs a qualitative and descriptive method. The research involved several methods, including sample collection, media preparation, isolation and selection of thermophilic bacterial isolates, purification of thermophilic bacterial isolates, selection of isolates producing amylase enzymes, identification of selected isolates (motility test, biochemical test, gram staining), and molecular identification. The research yielded seven isolates, and isolate code A6 demonstrated the potential to produce amylase enzymes with a clear zone size of 23,26 mm, bacillus, gram positive, and positive motility. Subsequently, molecular identifidation was carried out in which, based on the results of phylogenetic tree reconstractiopisalate A6 was related to the bacteria Bacillus cereus strain MD 152, which belongs to the Bacillus cereus bacteria