Academic literature on the topic 'B. amyloliquefaciens'
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Journal articles on the topic "B. amyloliquefaciens"
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Zheng, Yangyang, Xudong Wang, Siyuan Liu, Kewei Zhang, Zhibo Cai, Xiuling Chen, Yao Zhang, Jiayin Liu, and Aoxue Wang. "The Endochitinase of Clonostachysrosea Expression in Bacillus amyloliquefaciens Enhances the Botrytis cinerea Resistance of Tomato." International Journal of Molecular Sciences 19, no. 8 (July 30, 2018): 2221. http://dx.doi.org/10.3390/ijms19082221.
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To investigate whether the ech42 gene in Clonostachysrosea can improve the biocontrol efficacy of Bacillus amyloliquefaciens and its molecular mechanism. Compared to the wild type, the B. amyloliquefaciens transformed with the ech42 gene exhibited higher chitinase activity. The B. amyloliquefaciens-ech42 also showed significantly higher biocontrol efficiency compared to Botrytiscinerea when tomato plants were pre-treated with B. amyloliquefaciens-ech42. No significant difference in biocontrol efficiency was observed between the wild type and B.amyloliquefaciens-ech42 when tomato plants were first infected by Botrytiscinerea. In addition, the activity of the defense-related enzyme polyphenol oxidase, but not superoxide dismutase, was significantly higher in B. amyloliquefaciens-ech42 than in the wild type. The ech42 enhances the biocontrol efficiency of B.amyloliquefaciens by increasing the capacity of preventative/curative effects in plants, rather than by killing the pathogens.
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Dunlap, Christopher A., Soo-Jin Kim, Soon-Wo Kwon, and Alejandro P. Rooney. "Phylogenomic analysis shows that Bacillus amyloliquefaciens subsp. plantarum is a later heterotypic synonym of Bacillus methylotrophicus." International Journal of Systematic and Evolutionary Microbiology 65, Pt_7 (July 1, 2015): 2104–9. http://dx.doi.org/10.1099/ijs.0.000226.
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The rhizosphere-isolated bacteria belonging to the Bacillus amyloliquefaciens subsp. plantarum and Bacillus methylotrophicus clades are an important group of strains that are used as plant growth promoters and antagonists of plant pathogens. These properties have made these strains the focus of commercial interest. Here, we present the draft genome sequence of B. methylotrophicus KACC 13105T ( = CBMB205T). Comparative genomic analysis showed only minor differences between this strain and the genome of the B. amyloliquefaciens subsp. plantarum type strain, with the genomes sharing approximately 95 % of the same genes. The results of morphological, physiological, chemotaxonomic and phylogenetic analyses indicate that the type strains of these two taxa are highly similar. In fact, our results show that the type strain of B. amyloliquefaciens subsp. plantarum FZB42T ( = DSM 23117T = BGSC 10A6T) does not cluster with other members of the B. amyloliquefaciens taxon. Instead, it clusters well within a clade of strains that are assigned to B. methylotrophicus, including the type strain of that species. Therefore, we propose that the subspecies B. amyloliquefaciens subsp. plantarum should be reclassified as a later heterotypic synonym of B. methylotrophicus.
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Nechypurenko, O., M. Kharkota, and L. Avdeeva. "Safety of carotene-producing strains Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113 for homoiothermal animals." Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology 68, no. 3 (2014): 21–24. http://dx.doi.org/10.17721/1728_2748.2014.68.21-24.
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It was detected that the strains of carotenoid-producing Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113 are safe for warm-blooded animals, also avirulent and do not show toxic and toxigenic effect. It was determined the absence of the ability of strains of Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113 to produce putrescine and cadaverine. In experiments on mice was shown that oral and intraperitoneal administration of suspensions of the strains of bacilli, containing 15 billion cells/mouse did not cause the death of experimental animals and did not lead to pathologic changes in the tissues of their bodies. Thus, strains Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113 are non-pathogenic and safe for warm-blooded animals.
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Ranjith, Sellappan, Thangavel Kalaiselvi, Muruganagounder Muthusami, and Uthandi Sivakumar. "Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (Spodoptera frugiperda) in Maize." Microorganisms 10, no. 9 (September 16, 2022): 1850. http://dx.doi.org/10.3390/microorganisms10091850.
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Maize is an important cereal crop which is severely affected by Spodoptera frugiperda. The study aims to identify endophytic bacteria of maize root and leaf apoplastic fluid with bioprotective traits against S. frugiperda and plant growth promoting properties. Among 15 bacterial endophytic isolates, two strains—namely, RAF5 and LAF5—were selected and identified as Alcaligenes sp. MZ895490 and Bacillus amyloliquefaciens MZ895491, respectively. The bioprotective potential of B. amyloliquefaciens was evaluated through bioassays. In a no-choice bioassay, second instar larvae of S. frugiperda fed on B. amyloliquefaciens treated leaves (B+) recorded comparatively lesser growth (1.10 ± 0.19 mg mg−1 day−1) and consumptive (7.16 ± 3.48 mg mg−1 day−1) rates. In larval dip and choice bioassay, the same trend was observed. In detached leaf experiment, leaf feeding deterrence of S. frugiperda was found to be greater due to inoculation with B. amyloliquefaciens than Alcaligenes sp. The phenolics content of B. amyloliquefaciens inoculated plant was also found to be greater (3.06 ± 0.09 mg gallic acid g−1). However, plant biomass production was more in Alcaligenes sp inoculated treatment. The study thus demonstrates the potential utility of Alcaligenes sp. and B. amyloliquefaciens for improving growth and biotic (S. frugiperda) stress tolerance in maize.
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Fang, Haitian, Huiyan Liu, Ning Chen, Chenglin Zhang, Xixian Xie, and Qingyang Xu. "Site-directed mutagenesis studies on the uridine monophosphate binding sites of feedback inhibition in carbamoyl phosphate synthetase and effects on cytidine production by Bacillus amyloliquefaciens." Canadian Journal of Microbiology 59, no. 6 (June 2013): 374–79. http://dx.doi.org/10.1139/cjm-2012-0758.
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A major problem when pyrimidine de novo biosynthesis is used for cytidine production is the existence of many negative regulatory factors. Cytidine biosynthesis in Bacillus amyloliquefaciens proceeds via a pathway that is controlled by uridine monophosphate (UMP) through feedback inhibition of carbamoyl phosphate synthetase (CPS), the enzyme that converts CO2, NH3, and glutamine to carbamoyl phosphate. In this study, the gene carB encoding the large subunit of CPS from B. amyloliquefaciens CYT1 was site directed, and the UMP binding sites of feedback inhibition in Bam-CPS are described. The residues Thr-941, Thr-970, and Lys-986 in CPS from B. amyloliquefaciens were subjected to site-directed mutagenesis to alter UMP’s feedback inhibition of CPS. To find feedback-resistant B. amyloliquefaciens, the influence of the T941F, T970A, K986I, T941F/K986I, and T941F/T970A/K986I mutations on CPS enzymatic properties was studied. The recombinant B. amyloliquefaciens with mutated T941F/K986I and T941F/T970A/K986I CPS showed a 3.7- and 5.7-fold increase, respectively, in cytidine production in comparison with the control expressing wild-type CPS, which was more suitable for further application of the cytidine synthesis. To a certain extent, the 5 mutations were found to release the enzyme from UMP inhibition and to improve B. amyloliquefaciens cytidine-producing strains.
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Ahsan, Taswar, Chaoqun Zang, Shuyi Yu, Xue Pei, Jinhui Xie, Ying Lin, Xiaozhou Liu, and Chunhao Liang. "Screening, and Optimization of Fermentation Medium to Produce Secondary Metabolites from Bacillus amyloliquefaciens, for the Biocontrol of Early Leaf Spot Disease, and Growth Promoting Effects on Peanut (Arachis hypogaea L.)." Journal of Fungi 8, no. 11 (November 20, 2022): 1223. http://dx.doi.org/10.3390/jof8111223.
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A novel Bacillus amyloliquefaciens BAM strain, with novel fermentation nutrient mediums and compositions, could produce potent antifungal secondary metabolites, as the existing strains face resistance from fungus pathogens. In the current study, we introduced two novel nutrient mediums for the fermentation process, semolina and peanut root extract, as carbon and nitrogen sources in order to maximize the antifungal effects of B. amyloliquefaciens against Cercaspora arachidichola to control early leaf spot disease in peanuts. Based on a single-factor test and the central composite design of response surface methodology, the optimum fermentation medium for Bacillus amyloliquefaciens antagonistic substance was determined, containing 15 gm/L of semolina flour, 12.5 gm/L of beef extract, and 0.5 gm/L of magnesium sulfate, which inhibited the fungal growth by 91%. In vitro, antagonistic activity showed that the fermentation broth of B. amyloliquefaciens BAM with the optimized medium formulation had an inhibition rate of (92.62 ± 2.07)% on the growth of C. arachidichola. Disease control effects in pot experiments show that the pre-infection spray of B. amyloliquefaciens BAM broth had significant efficiency of (92.00 ± 3.79)% in comparison to post-infection spray. B. amyloliquefaciens BAM broth significantly promoted peanut plant growth and physiological parameters and reduced the biotic stress of C. archidechola. Studies revealed that B. amyloliquefaciens BAM with a novel fermentation formulation could be an ideal biocontrol and biofertilizer agent and help in early disease management of early leaf spots in peanuts.
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Wei, Xubiao, Xiudong Liao, Jun Cai, Zhaojun Zheng, Lulu Zhang, Tingting Shang, Yu Fu, Cong Hu, Lei Ma, and Rijun Zhang. "Effects of Bacillus amyloliquefaciens LFB112 in the diet on growth of broilers and on the quality and fatty acid composition of broiler meat." Animal Production Science 57, no. 9 (2017): 1899. http://dx.doi.org/10.1071/an16119.
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An experiment was conducted to investigate the effects of Bacillus amyloliquefaciens LFB112 in the diet on growth of broilers and on the quality and fatty acid composition of broiler meat. 440 1-day-old Arbor Acres male broiler chicks were randomly allocated into five dietary treatments with eight replicates in a completely randomised design for 42 days. The treatments were as follows: basal diet (control) or the basal diet supplemented with 107 colony-forming units (CFU) B. amyloliquefaciens/kg (B7), 108 CFU B. amyloliquefaciens/kg (B8), 109 CFU B. amyloliquefaciens/kg (B9) or 40 mg aureomycin/kg (antibiotic). The results showed that B. amyloliquefaciens-supplemented groups had greater (P < 0.05) average daily gain during Days 1 to 21, Days 22 to 42 and Days 1 to 42, and improved (P < 0.05) feed conversion rate during Days 1 to 42 compared with the control. Broilers in the B7 and B9 groups had higher (P < 0.05) average daily feed intake than those in the control group in all experimental phases. Broilers fed either B. amyloliquefaciens or antibiotic diet showed higher (P < 0.05) a* value, b* value and lower L* value in the breast muscle than those in the control group. Dietary B. amyloliquefaciens supplementation increased (P < 0.05) the contents of C14:1, C16:1c, C18:1t, C18:1c, C20:1n-9, total monounsaturated fatty acids, C18:2c, C18:3n-3, C20:2n-6, C20:3n-6, C20:3n-3, C20:4n-6, C20:5n-3, C22:6n-3 and total polyunsaturated fatty acids as well as ratio of polyunsaturated fatty acids to saturated fatty acids in breast muscle and the concentrations of C17:1, C20:1n-9, C18:2t-9, t-12, C20:2n-6, C20:3n-6, C20:3n-3, C20:4n-6, C20:5n-3 and C22:6n-3 in thigh muscle of broilers. The results indicated that B. amyloliquefaciens improved broilers’ growth performance, meat quality and fatty acid composition.
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Nan, Jing, Shaoran Zhang, and Ling Jiang. "Antibacterial Potential of Bacillus amyloliquefaciens GJ1 against Citrus Huanglongbing." Plants 10, no. 2 (January 29, 2021): 261. http://dx.doi.org/10.3390/plants10020261.
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Citrus huanglongbing (HLB) is a destructive disease caused by Candidatus Liberibacter species and is a serious global concern for the citrus industry. To date, there is no established strategy for control of this disease. Previously, Bacillus amyloliquefaciens GJ1 was screened as the biocontrol agent against HLB. In this study, two-year-old citrus infected by Ca. L. asiaticus were treated with B. amyloliquefaciens GJ1 solution via root irrigation. In these plants, after seven irrigation treatments, the results indicated that the photosynthetic parameters, chlorophyll content, resistance-associated enzyme content and the expression of defense-related genes were significantly higher than for the plants treated with the same volume water. The content of starch and soluble sugar were significantly lower, compared to the control treatment. The parallel reaction monitoring (PRM) results revealed that treatment with B. amyloliquefaciens GJ1 solution, the expression levels of 3 proteins with photosynthetic function were upregulated in citrus leaves. The accumulation of reactive oxygen species (ROS) in citrus leaves treated with B. amyloliquefaciens GJ1 flag22 was significantly higher than untreated plants and induced the defense-related gene expression in citrus. Finally, surfactin was identified from the fermentation broth of B. amyloliquefaciens GJ1 by high-performance liquid chromatography. These results indicate that B. amyloliquefaciens GJ1 may improve the immunity of citrus by increasing the photosynthesis and enhancing the expression of the resistance-related genes.
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Wang, Da-Cheng, Chun-Hao Jiang, Li-Na Zhang, Lin Chen, Xiao-Yun Zhang, and Jian-Hua Guo. "Biofilms Positively Contribute to Bacillus amyloliquefaciens 54-induced Drought Tolerance in Tomato Plants." International Journal of Molecular Sciences 20, no. 24 (December 12, 2019): 6271. http://dx.doi.org/10.3390/ijms20246271.
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Drought stress is a major obstacle to agriculture. Although many studies have reported on plant drought tolerance achieved via genetic modification, application of plant growth-promoting rhizobacteria (PGPR) to achieve tolerance has rarely been studied. In this study, the ability of three isolates, including Bacillus amyloliquefaciens 54, from 30 potential PGPR to induce drought tolerance in tomato plants was examined via greenhouse screening. The results indicated that B. amyloliquefaciens 54 significantly enhanced drought tolerance by increasing survival rate, relative water content and root vigor. Coordinated changes were also observed in cellular defense responses, including decreased concentration of malondialdehyde and elevated concentration of antioxidant enzyme activities. Moreover, expression levels of stress-responsive genes, such as lea, tdi65, and ltpg2, increased in B. amyloliquefaciens 54-treated plants. In addition, B. amyloliquefaciens 54 induced stomatal closure through an abscisic acid-regulated pathway. Furthermore, we constructed biofilm formation mutants and determined the role of biofilm formation in B. amyloliquefaciens 54-induced drought tolerance. The results showed that biofilm-forming ability was positively correlated with plant root colonization. Moreover, plants inoculated with hyper-robust biofilm (ΔabrB and ΔywcC) mutants were better able to resist drought stress, while defective biofilm (ΔepsA-O and ΔtasA) mutants were more vulnerable to drought stress. Taken altogether, these results suggest that biofilm formation is crucial to B. amyloliquefaciens 54 root colonization and drought tolerance in tomato plants.
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Jinno, Cynthia N., and Yanhong Liu. "109 Awardee Talk: Dietary Supplementation of Bacillus Amyloliquefaciens Influenced Systemic Immunity and Intestinal Microbiome of Weaned Pigs." Journal of Animal Science 100, Supplement_2 (April 12, 2022): 51. http://dx.doi.org/10.1093/jas/skac064.080.
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Abstract Supplementation of various Bacillus spp. has shown to improve growth performance, immune responses, and gut health of newly weaned pigs. The present study investigated the effects of dietary supplementation of 109 CFU/kg of Bacillus (B.) amyloliquefaciens on growth performance, diarrhea, systemic immunity, and intestinal microbiome of weaned pigs with or without enterotoxigenic Escherichia coli (ETEC) challenge. An antibiotic treatment was also included in the experiment to compare the efficacy of B. amyloliquefaciens vs. carbadox (50 mg/kg). ETEC challenge reduced (P < 0.05) growth rate but increased (P < 0.05) the frequency of diarrhea in pigs throughout the experiment. Supplementation of B. amyloliquefaciens tended (P < 0.10) to increase growth performance but had limited impacts on diarrhea of weaned pigs. ETEC challenge increased (P < 0.05) white blood cell (WBC) count on d 7 and 21 post-inoculation (PI), while supplementation of B. amyloliquefaciens tended (P < 0.10) to reduce WBC on d 7 PI and reduced (P < 0.05) WBC on d 21 PI, compared with control. No differences were observed in performance, diarrhea, and systemic immunity of pigs when comparing B. amyloliquefaciens with carbadox. Supplementation of B. amyloliquefaciens and antibiotics had different impacts on intestinal microbiome. ETEC challenge increased (P < 0.05) relative abundance of Lactobacillus in feces on d 7 PI but reduced (P < 0.05) relative abundance of Prevotella in feces on d 21 PI. Prior ETEC challenge, Lachnospiraceae was less (P < 0.05) abundant in feces of pigs supplemented with B. amyloliquefaciens than pigs in carbadox group on d 0 before ETEC infection. The relative abundance of Enterobacteriaceae in fecal samples was the lowest (P < 0.05) in pigs supplemented with carbadox among all treatment on d 0. On d 14 and 21 PI, the relative abundance of Actinobacteria in feces was the lowest (P < 0.05) in carbadox treatment among all treatment of the experiment. Ileal digesta were also collected on d 21 PI to analyze intestinal microbiota diversity changes among treatments. The Shannon diversity index was greater (P < 0.05) in ileal digesta of pigs supplemented with B. amyloliquefaciens than pigs supplemented with carbadox. ETEC reduced (P < 0.05) the relative abundance of Firmicutes and Lactobacillus and increased (P < 0.05) the relative abundance of Proteobacteria and Lachnospiraceae in ileal digesta. Under ETEC challenge, pigs supplemented with B. amyloliquefaciens had lower (P < 0.05) abundance of Firmicutes and Clostridiaceae and greater (P < 0.05) abundance of Lachnospiraceae in the ileum than pigs supplemented with carbadox. In conclusion, supplementation of B. amyloliquefaciens had similar impacts on growth performance and systemic immunity but had different impacts on intestinal microbiota of weaned pigs in comparison to carbadox.
Dissertations / Theses on the topic "B. amyloliquefaciens"
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Budiharjo, Anto. "Plant-bacteria interactions." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16333.
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Bacillus amyloliquenaciense FZB42 ist ein bekanntes Pflanzenwachstum-stimulierendes Rhizobakterium. Es produziert neben einer Vielzahl an Sekundärmetaboliten mit antibakterieller und antifungaler Wirkung, auch das Pflanzenhormon IAA. Obwohl viele dieser Mechanismen diskutiert werden, ist wenig darüber bekannt, auf welche Weise die Bakterien das Pflanzenwachstum fördern. In dieser Arbeit wurde eine Transposonmutagenese mithilfe des ‘mariner-transposons’ durchgeführt, und so eine Transposonbibliothek erstellt. Diese wurde dann auf geeignete Phänotypen untersucht, um die Gene zu finden, welche bestimmte Phänotypen verursachen. So konnten drei Mutanten erzeugt werden, die auf Grund der gestörten Biofilmbildung und der Fähigkeit zu schwärmen die Pflanzenwurzeln nicht mehr kolonialisieren konnten. Eine solche degU-Mutante, welche in der Biofilmbildung und ‚Swarming’ defizitär war und zwei Mutanten (yusV und pabB), die eine Beeinträchtigung in der Biofilmbildung aufwiesen, konnten durch Komplementation und Retransformation bestätigt werden. Mithilfe des Lemna-Biosystems und anderer Analysen mit A. thaliana konnten drei Gene bei B. amyloliqufaciens FZB42 gefunden werden, die wichtig für die Förderung des Pflanzenwachstums sind. Koloniesierungsexperimente der Wurzeln von A. thaliana mit diesen Mutanten zeigten deutlich verändertes Wachstum, verglichen mit dem Wildtypstamm. Ein weiteres Ziel dieser Arbeit war es neue Antibiotika in Mutanten, die in ihren nicht-ribosomalen Synthesen blockiert sind, zu finden. So konnten durch die Untersuchungen der Transposonbibliothek der Mutanten zwei neue Antibiotika entdeckt werden. Genauere Analysen dieser Antibiotika bestätigten, dass es sich um ein neues Bacteriocin (Amylocyclicin A) und ein neues Thiazol/Oxazole-modifiziertes Microcin (Plantazolicin) handelt. Die abschließenden Arbeiten beschäftigten sich dann mit Untersuchungen von Genen, welche für die Produktion von Substanzen gegen Nematoden verantwortlich sind. Hierbei konnten vier Mutanten gefunden werden, die durch eine Transposoninsertion eine schlechtere.Bacillus amyloliqufaciens FZB42 has been known as PGPR which has an impressive effect to improve plant growth. It produces not only vast array of secondary metabolites with antibacterial and antifungal activities, but also produces the plant hormone IAA. Although many mechanisms have been elucidated, our knowledge about basic molecular mechanisms responsible for its beneficial action is far from complete. In this study, transposon mutagenesis based on mariner tranposon was applied to generate tranposon library which then was screened to identify the genes involved in plant growth-promoting activity. Three mutants that were impaired in their ability to colonize plant surface due to defects in biofilm formation and swarming motility were found. One mutant (degU mutant) showed defect in biofilm formation and swarming motility, as well, two mutants (yusV mutant and pabB mutant) impaired in biofilm formation were confirmed by complementation and retransformation. Screening by the Lemna biosystem and further assays with A. thaliana revealed three genes responsible for reduction in plant growth promoting activity of B. amyloliqufaciens FZB42. Colonization studies of these mutants in A. thaliana roots revealed patterns different to the wild type. A further issue pursued in this study was to discover new antibiotics using a mutant which has been blocked in its nonribosomally pathway. Screening of tranposon librabries from this mutant led to the finding of two novel ribosomally synthesized antibiotics. Further characterization revealed that these new antibiotics belonged to a novel bacteriocin (Amylocyclicin A) and a novel thiazole/oxazole-modified microcin (Plantazolicin). Last work in this study was looking for genes responsible for nematocidal production. Four mutants which showed reduction in nematocidal activity due to transposon insertion were found.
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Kalyon, Bahar [Verfasser], Roderich [Akademischer Betreuer] SüßMuth, and Hans-Peter [Akademischer Betreuer] Fiedler. "Fermentation, Isolierung und Strukturaufklärung der ribosomal synthetisierten Thiazol/Oxazol-Peptide Plantazolicin A und B aus Bacillus amyloliquefaciens FZB42 und Strukturaufklärung der Langkocycline aus Streptomyces sp. Acta 3034 / Bahar Kalyon. Gutachter: Roderich Süßmuth ; Hans-Peter Fiedler." Berlin : Technische Universität Berlin, 2014. http://d-nb.info/1067384782/34.
Full textBook chapters on the topic "B. amyloliquefaciens"
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Bawden, M. J., T. Litjens, T. R. Hercus, B. K. May, and W. H. Elliott. "Extracellular Protease Production by B. Amyloliquefaciens." In Extracellular Enzymes of Microorganisms, 89–92. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4684-1274-1_11.
Full textConference papers on the topic "B. amyloliquefaciens"
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Abreu, Luciana De Paiva Santos, Carlos Eduardo De Souza Teodoro, and Ana Paula Martinazzo. "ATIVIDADE ANTIFÚNGICA IN VITRO DO SOBRENADANTE DA BACTÉRIA BACILLUS AMYLOLIQUEFACIENS SOBRE O FUNGO ASPERGILLUS FLAVUS." In II Congresso Brasileiro de Ciências Biológicas On-line. Revista Multidisciplinar de Educação e Meio Ambiente, 2021. http://dx.doi.org/10.51189/rema/1300.
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Introdução: O Aspergillus flavus é um fungo endêmico do solo, foi identificado como grande produtor de aflatoxina, a contaminação ocorre tanto durante o armazenamento, quanto ainda na fase de plantio. O milho é uma das culturas mais afetadas pela espécie, levando à sua contaminação por aflatoxina, e posteriormente esta é repassada aos animais que o consomem. O uso de fungicidas químicos é amplamente aplicado, no entanto esta prática pode levar à contaminação do grão. O biocontrole vêm como uma alternativa sustentável para substituir os defensivos agrícolas químicos. Objetivos: Na bactéria Bacillus amyloliquefaciens–VR002 (BA) foram identificadas características relacionadas ao seu papel como antagonista de microrganismos patogênicos. Analisou-se a atuação do sobrenadante da bactéria BA-VR002 no crescimento micelial do fungo Aspergillus flavus. Material e métodos: Os experimentos ocorreram no Laboratório de Biotecnologia/UFF-VR. A bactéria foi crescida utilizando 150mL do meio Luria Bertani esterilizado e inoculado com a cultura de BA-VR002 deixando-a crescer por 48h em estufa à 37ºC e 170rpm. Após o tempo de fermentação, o caldo foi centrifugado à 3500rpm, à 4ºC por 30min, filtrado em filtro de 22μm para um erlenmeyer estéril. Este filtrado foi então misturado ao meio ágar LB à 50±10ºC nas proporções de: 0%(v/v); 10%(v/v); 20%(v/v) e 30%(v/v). Este foi vertido em placas de Petri estéreis, após o endurecimento do ágar foram dispostos discos de micélio contendo o fungo A. flavus no centro das placas incubando-as em estufa à 30ºC por 5 dias. Os testes foram feitos em sextuplicata, e a área de crescimento foi calculada por meio do programa ImageJ. Resultados: Os resultados mostram que o percentual de inibição fúngico máximo ocorreu na concentração de 30% de sobrenadante, atingindo 96,4% de inibição do crescimento micelial quando comparado ao controle. Na concentração de 10% a inibição foi de 14,8%, na de 20% foi de 84,6%, confirmando a presença das substancias antifúngicas produzidas pela BA-VR002. Conclusão: O sobrenadante da bactéria B. amyloliquefaciens-VR002 contém substâncias antifúngicas, sendo a concentração de 30% a mais eficaz, mostrando-se como um potencial agente de controle do fungo A. flavus.