Dissertations / Theses on the topic 'B-720'

Dans le cadre du califat Umayyade nous avons étudié le règne de Umar II. Pour essayer de tracer une esquisse de son système politique qui consista essentiellement à tempérer toutes les tendances anti-umayyade au sein de l'état. Pour ce qui est de sa politique extérieure parallèlement au mouvement des conquêtes, il adopta un autre procédé pour la propagande de l'islam : la correspondance entre lui et l'empereur byzantin Léon III, entre lui et les souverains de l'Inde et du Sind. Pour ce qui est de l'administration il réorganisa la répartition des provinces et nomma de nouveaux gouverneurs et employés qui furent sujets à un contrôle sévère bien que la plupart d'entre eux soient des faqihs et des muhaddiths. Il instaura aussi de nouveaux (diwan) bureaux. Dans le domaine fiscale il interdit la perception de la capitation des nouveaux convertis à l'islam et interdit aussi la vente ou la concession des terres soumises à l'impôt foncier. Il était pour l'idée selon laquelle l'imam jouit d'un plein pouvoir sur le statut des terres soumises à l'impôt foncier (kharadj). Il faut souligner que la politique de Umar II était en quelques sortes une poursuite plus radicale de celle de son prédécesseur Suleyman qui a préparé l'avènement de son cousin Umar II à l'aide du faqih de la cour radja'b. Haywa. L'aspect fondamental de la personalite de "umar ii est que ce souverain Umayyade était un faqih et muhaddith (savant et transmetteur du hadith) médinois qui nous a laissé sans doute le premier musnad (recueil de hadith classifié selon les transmetteurs) qu'il rédigea officiellement
It is within the context of the Umayyad califate that one has endeavored to study the reign of this sovereign. In order to lay out an outline of his rule it is essential that we study his internal policy. In this particular field he managed to suppress all anti-Umayyad tendencies which were prevalent under the Damascus regime. With regard to his foreign policy he was in favor of the conquest and though his correspondence. With the byzantine emperor Leo III and the sovereigns of India and Sind. He also stands for propaganda of Islam. As for the administration of the sovereign he reorganized the reallocation of the provinces to some extent and he also appointed new governors and created new offices (diwan). As for as his tax system was concerned, he abolished the poll-tax collection from those who had been newly converted to Islam. Thus he prohibited the sale and concession of the land which was subjected to the land tax. One must emphasize that the political system of Umar was in same ways a radical continuation of his predecessor Suleiman who together with the court's faqihradja" b. Haywa contrived the succession of Umar II. The basic feature of his personality was that he was in every respect a medinian faih and muhaddith. He was probably the author of the first musnad (a collection of hadith which is classified according to the transmitter) compiled by him

Nous présentons une nouvelle approche entière, composée et critique de la littérature et l'historiographie sur les différentes dimensions du personnage de ʿUmar II. Entière parce qu'il s'agit de recomposer avec diverses intra- et extra-islamiques appréciations de l'homme, son entourage et ses cours/mağālis. Composée et critique également car il s’agit de mener un chantier complet sur la littérature classique et l’approche critique moderne afin de saisir la réelle contribution de ʿUmar II à la fondation de l'islam comme religion (droit, théologie, ḥadîṯ, ascétisme) et comme culture (politique, poésie et Adab), en tirant profit de chacune des lectures sur ce personnage. En effet, ʿUmar II est présenté notamment comme calife juste et homme politique modèle dans la littérature des cours et de l’Adab. Nous traitons d’autres dimensions de ce personnage de l’islam primitif, très peu étudiées ou timidement exposées, et parfois contestées ; celle du juriste-traditionniste jouit du statut honorifique de muğtahid/muğaddid, rénovateur du dīn, dont la contribution au droit islamique ancien est intensivement soulignée. Malgré une très courte carrière politique et juridique, son autorité en matière légale a été rapidement reconnue. Bien avant le milieu du second siècle son droit a été intégré dans les maḏhab-s primitifs et classiques. Son Musnad, récemment édité, est d’un apport tout particulier sur les anciennes méthodes de transmission de la tradition et sur le milieu médinois en général. Un phénomène aurait vu le jour en son temps, l’ascétisme, et la tradition présente le calife comme catalyseur de ce mouvement, son héros et l’inspirateur d’une expression poétique à travers sa conduite éthique et politique exemplaire, sans omettre les dimensions eschatologiques de ʿUmar le Mahdī, messie sauveur qui aurait fini comme martyr (šahīd), en lien avec l’esprit qui régnait depuis la fin du premier siècle et dont on trouve les traces dans plusieurs sources arabes et étrangères
An entirely new critical and complex approach to the literature and historiography built around 'Umar II' character’s various dimensions. It is a thorough reconstruction based on various intra- and extra-Islamic assessments of the man, his surroundings and his courtyard(s)/mağālis. It is complex and critical as it opens a large undertaking on classical literature and modern critical approaches in order to capture the real contribution of ʿUmar II to the foundation of Islam as a religion (law, theology, ḥadīṯ, asceticism) and as a culture (politics, poetry and Adab) while taking advantage of various analysis of his persona. In fact, ʿUmar II is presented in particular as a fair Caliph and a role model politician in the literature of court and "Adab". We address other undiscovered or, sometimes, timidly exposed and challenged aspects of this character of primitive Islam; a jurist-traditionalist enjoying the honorary status of muğtahid/muğaddid and dīn renovator which contributed massively to the ancient Islamic legislation. Despite his short political and legislative carrier, his influence in law-making was recognised rapidly. Even earlier than mid second century, his legislation was adopted and integrated in primitive and classical maḏhab-s. His Musnad, recently edited, has particularly improved traditional methods of transmitting tradition and influenced the Medina settings in general. Asceticism, as phenomenon, has seen the light in his era first and portrayed the Caliph as the catalyst of this movement, its hero and the inspiration of a poetic expression through his ethical and political exemplary conduct, without omitting the eschatological dimensions of ʿUmar the Mahdī, the Messiah and saviour who would have ended as a martyr (šahīd), in connection with the spirit that prevailed since the end of the first century and which traces are found in several Arab sources

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To evaluate the seroepidemiology profile of the hepatitis B virus infection, and the response to Butang® vaccine in adolescents from a low-income region in the Metropolitan Area of Goiânia city Goiás 664 individuals with 12 to 19 years old were interviewed regarding socio-demographic and HBV risk factors After blood samples were collected and serum samples were tested for HBV markers (HBsAg, anti-HBs and anti-HBc) by enzyme-linked immunosorbent assay (ELISA) HBsAg positive samples were subtyped by ELISA and HBV DNA detected by polymerase chain reaction Positive samples were genotyped by restriction fragment length polymorphism (RFLP) method Three doses of 20μg of the Butang® vaccine were offered to all susceptible adolescents and the vaccine response was evaluated nearly 45 days after A global prevalence of 5.9% (CI 95%: 4.3 8.0) was found ranging from to 2.4% (CI 95%: 0.7 - 6.3) to 17.3% (CI 95%:11.0 - 26.0) All HBsAg positive adolescents were infected with HBV isolates of subtype adw2 and genotype A Age of 16 to 19 years attending in evening classes school B birth in other state and body piercing were independently associated to HBV positivity A total of 304 adolescents were susceptible to hepatitis B virus Of them 182 compliance with the full vaccine scheme and in 170 the vaccine response was evaluated All developed protector anti-HBs titers being the majority with titers higher than 1.000 mUI/mL The anti-HBs geometric means titers (GMT) were equal to 4.344 mUI/mL (CI 95%: 3.492 5.404) These findings ratify the importance of hepatitis B vaccine to adolescents mainly to low income ones whose social conditions should support the viral dissemination The administration of the present scheme of Butang® should guarantee protective anti-HBs levels to individuals at a critical time for hepatitis B acquiring such as latter adolescence and adulthood
Para investigar o perfil soroepidemiológico da infecção pelo vírus da hepatite B e avaliar a soroconversão à vacina Butang® em adolescentes da periferia da Região Metropolitana de Goiânia Goiás 664 indivíduos de 12 a 19 anos foram entrevistados sobre dados pessoais e fatores de risco para a infecção pelo HBV A seguir foram coletadas amostras sanguíneas para detecção dos marcadores sorológicos do HBV (HBsAg, antiHBs e anti-HBc) pelo ensaio imunoenzimático (ELISA) As amostras HBsAg reagentes foram subtipadas por ELISA e submetidas à detecção do HBV DNA pela reação em cadeia da polimerase sendo as positivas genotipadas por análise do polimorfismo de fragmentos de restrição (RFLP) A todos os adolescentes suscetíveis foram oferecidas três doses de 20 μg da vacina Butang® e a resposta vacinal detectada cerca de 45 dias após a última dose Uma prevalência global de 5,9% (IC 95%: 4,3 8,0) foi encontrada, variando de 2,4% (IC 95%: 0,7-6,3) a 17,3% (IC 95%:11,0-26,0) Todos os adolescentes HBsAg positivos foram infectados com amostras do subtipo adw2 e genótipo A Possuir 16 a 19 anos de idade, estudar no período noturno na escola B ser natural de outro estado e usar body piercing foram fatores independentemente associados à positividade ao HBV Um total de 304 adolescentes eram suscetíveis ao vírus da hepatite B Destes 182 receberam o esquema completo da vacina Butang® e em 170 foi possível avaliar a resposta vacinal Todos desenvolveram anticorpos anti-HBs em níveis protetores sendo a maioria com títulos superiores a 1.000 mUI/mL A média geométrica dos títulos (GMT) de anti-HBs foi igual a 4.344 mUI/mL (IC 95%: 3.492 5.404) Os resultados desta investigação ratificam a importância da vacinação contra hepatite B na população de adolescentes principalmente para os de baixa renda cujas condições sociais parecem favorecer a disseminação viral A utilização da Butang® com o presente esquema deve garantir títulos protetores de anti-HBs em uma fase crítica para infecção pelo HBV como a adolescência e vida adulta jovem

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This current study brings as main topic the family agriculture and management strategies used by family farmers. Family agriculture is responsible for production of 70% of foods consumed by brazilians, 64% of occupations in rural areas and 10% of brazilian GDP, contradicting scholars such as Kautsky, who predicted the family farmer extinction before the accelerated growth of agribusiness and these smallholders are still surviving in the midst of a dynamic and complex market. Over the years, many public policies were developed aiming to promote the growth of agricultural segment, but such measures were predominantly benefiting great enterprises in rural areas. After years of struggle, in 1996 was created the National Program for Sustainable Family Agriculture (PRONAF in Portuguese), which emerged aiming to promote the development for these small rural enterprises, as well as providing improvements in quality of life of these family farmers who contribute so much to the food production. Among credit lines, this work standeth in PRONAF B, a microcredit line directed to low incomes family farmers, which allows access to low values credit offering tax compliance discounts and reaching up to 25% of the value taken. However, even with so many incentives for tax compliance, PRONAF B had high elinquency rates, which led to the creation of the Oriented Rural Microcredit Program (AGROAMIGO), which provides credit to family farmers benefiting from PRONAF B, with monitoring of credit agents assisting since the project preparation until the process is complete with the transaction settlement. In this context of lending policies directed to family farming, this research had as main goal analyzing how family farmers in Assu-Mossoro are using resources provided by PRONAF B from 2009 to 2014. In order to meet the proposed goal, it was applied a field research with qualitative, exploratory and descriptive approaches, adopting as data collection instrument an interview structured consisting of 18 questions. The study site was conducted in Assu- Mossoro territory composed of 14 counties, where were interviewed 5 family farmers benefited by AGROAMIGO within the period of the study. From the collected data, it was concluded that the family farmers benefited by AGROAMIGO in the study site do not use, in their great majority, management tools in their rural businesses, possibly due to the low level of schooling found among interviewees. This could be an indicator of a need for a more effective presence on the part of ATER in order to enable these farmers to undertake a management of their businesses so that obtain a sustainable development and improving the quality of life of those involved. It is possible to know more from the interviews conducted, such as the perception on the part of the beneficiaries about benefits offered by PRONAF. However, it is a latent need that still exists for the program improvement in order to achieve the goals proposed
O presente estudo traz como tema principal a agricultura familiar e as estratégias de gestão utilizadas pelos agricultores familiares. A agricultura familiar é responsável pela produção de 70% dos alimentos consumidos pelos brasileiros, 64% das ocupações no meio rural e 10% do PIB brasileiro, contradizendo estudiosos como Kautsky (1899), que previam a extinção do agricultor familiar diante do crescimento acelerado do agronegócio, esses pequenos produtores se mantém sobreviventes em meio a um mercado dinâmico e complexo. Ao longo do tempo, muitas políticas públicas foram desenvolvidas com o objetivo de promover o crescimento do segmento agrícola, porém tais medidas beneficiavam predominantemente os grandes empreendimentos rurais. Após anos de luta, em 1996 foi criado o Programa Nacional de Fortalecimento da Agricultura Familiar (PRONAF), que surgiu com intuito de promover desenvolvimento a esses pequenos empreendimentos rurais, bem como proporcionar melhorias na qualidade de vida desses agricultores familiares que tanto contribuem para a produção nacional de alimentos. Entre suas linhas de crédito, o presente trabalho se detém no PRONAF B, linha de microcrédito rural voltada para agricultores familiares de baixa renda, que permite acesso a crédito de valores baixos, dispondo de descontos de adimplência que podem chegar a até 25% do valor tomado. Porém, mesmo com tantos incentivos ao adimplemento, o PRONAF B obteve altos índices de inadimplência, o que levou à criação do Programa de Microcrédito Rural Orientado (AGROAMIGO), que concede crédito aos agricultores familiares beneficiários do PRONAF B, dispondo do acompanhamento de agentes de crédito que auxiliam desde a elaboração do projeto até a conclusão do processo quando da liquidação da operação. Nesse contexto das políticas de crédito voltadas para a agricultura familiar, a presente pesquisa traz como objetivo geral analisar como os agricultores familiares do território Açu-Mossoró utilizam os recursos concedidos pelo PRONAF B no período de 2009 a 2014. De forma a atender ao objetivo proposto, utilizou-se de uma pesquisa de campo de abordagem qualitativa, exploratória e descritiva, adotando como instrumento de coleta de dados a entrevista semiestruturada composta por 18 perguntas. O local de estudo foi o território Açu-Mossoró (RN), composto por 14 municípios, onde foram entrevistados 5 agricultores familiares beneficiados pelo AGROAMIGO dentro do período compreendido pela pesquisa. A partir dos dados coletados, pôde-se concluir que os agricultores familiares beneficiados pelo AGROAMIGO no território em estudo não utilizam, em sua grande maioria, ferramentas de gestão em seus empreendimentos rurais, possivelmente decorrentes do baixo grau de escolaridade encontrado entre os pesquisados, podendo esse vir a ser um indicador da necessidade de uma presença mais eficaz por parte dos órgãos de ATER no sentido de capacitarem esses agricultores familiares para realizarem uma gestão de seus empreendimentos de forma a obterem um desenvolvimento sustentável e melhoria da qualidade de vida de seus envolvidos. Pôde-se conhecer ainda, a partir das entrevistas realizadas, a percepção dos beneficiados acerca dos benefícios proporcionados pelo PRONAF, embora seja latente a necessidade de aprimoramento do programa para que possa alcançar os objetivos a que tem se proposto
2017-06-19

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Centro de Integração Empresa Escola (CIEE)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fundação de Apoio e Desenvolvimento ao Ensino, Pesquisa e Extensão Universitária no Acre (FUNDAPE)
Nesta dissertação são apresentados dois estudos envolvendo isolados clínicos de Acinetobacter baumannii que apresentaram mecanismos enzimáticos de resistência aos carbapenêmicos, a produção de carbapenemases do tipo OXA. O primeiro estudo avalia a relação genética de amostras de A. baumannii multirresistentes produtoras de OXA-23 provenientes de distintas cidades brasileiras. 91 amostras clínicas de A. baumannii foram isoladas em 17 centros médicos localizados nas cidades de São Paulo (SP), Rio de Janeiro (RJ), Belo Horizonte (MG), Porto Alegre (RS), Blumenau (SC), Curitiba (PR), São Luiz (MA) e Salvador (BA). Nesta coleção observamos altas taxas de resistência aos carbapenêmicos (91.2%) e presença do gene blaOXA-23-like em 83,5% dos isolados. O elemento de insersão ISAba1 à montante ao gene blaOXA-23 foi detectado em todos os 76 isolados de A. baumannii produtores de OXA-23. Nove grupos de genótipos foram observados entre os 76 isolados produtores de OXA-23. Nossos achados sugerem que o gene blaOXA-23 presente nestes isolados estava localizado DNA cromossomal. Três principais grupos (A, B e D) foram observados circulando em seis cidades das regiões sudeste e sul do Brasil, sendo que o genótipo predominante (A) apresentou relação clonal àquele primeiramente descrito na cidade de Curitiba, em 2003. Em contrapartida foi encontrado um único genótipo de A. baumannii produtor de OXA-23 na cidade de São Luís. Embora existam estudos brasileiros prévios reportando a disseminação de clones de A. baumannii pordutores de OXA-23 localmente, nenhum estudo brasileiro demonstrou antes, i) a análise comparativa dos genótipos originários de diferentes e distantes cidades brasileiras, ii) a provável localização do gene blaOXA-23 e iii) a presença do elemento ISAba1 à montante ao gene blaOXA-23,o que pode ter levado ao alto grau de resistência aos carbapenens observado. Desta maneira, o estudo demonstra a circulação de clones de A. baumannii produtores de OXA-23 no Brasil e enfatiza que medidas de controle de infecção são urgentemente necessárias para reduzir tanto a disseminação de isolados multirresistenes quanto o número de infecções causadas por este patógeno. O segundo estudo trata de um relato de caso de um paciente internado em um hospital da cidade de São Paulo, que apresentou uma infecção por A. baumannii produtor de OXA-72. O isolado em questão, A 30235, apresentou resistência a todos os antimicrobianos testados, com excessão à ampicilina/sulbactam tendo apresentado redução da sensibilidade a esta associação. Foi realizada com sucesso a transformação do plasmídeo presente no isolado A30235 na cepa de A. baumannii ATCC 19606. A confirmação da presença do gene blaOXA-72 no transformante evidenciou a localização plasmidial deste determinante de resistência. O plasmídio contendo o gene blaOXA-72 apresentou um peso molecular de aproximadamente 86 Kb. Neste estudo identificamos pela primeira vez no Brasil, um isolado clínico de A. baumannii produtor de OXA-72. A presença deste determinante de resistência codificado por um gene localizado em um elemento genético móvel demonstra a crescente diversidade de carbapenemase do tipo OXA no Brasil com potencial de disseminação. Medidas de controles adequadas deverão ser tomadas para evitar a disseminação de isolados produtores de OXA-72 entre os hospitais brasileiros, o que tem ocorrido com os isolados de A. baumannii produtor de OXA-23.
In this dissertation we present two studies involving carbapenem-resistant Acinetobacter baumannii clinical isolates due to the production of carbapenems modifying enzymes, the OXA-type carbapenemases. The first study aimed to determine the genetic relationship of multi-drug-resistant A. baumannii producing OXA-23 that was isolated in distinct Brazilian cities. A total of 91 A. baumannii clinical isolates were recovered from 17 medical centers located at eight cities, namely São Paulo (SP), Rio de Janeiro (RJ), Belo Horizonte (MG), Porto Alegre (RS), Blumenau (SC), Curitiba (PR), São Luiz (MA) and Salvador (BA). In this collection we observed high rates of carbapenems resistance (91.2%). Also, the blaOXA- 23-like gene was present in 83.5% of isolates. The insertion sequence ISAba1 was positioned upstream the blaOXA-23 gene in all OXA-23-producing A. baumannii identified. Nine clusters were observed among OXA-23 producers. Our fidings suggest that the blaOXA-23 gene was probably chromosomally-located in all isolates studied. Three clusters (A, B and D) were found in six cities from southeast and southern reagions of Brazil. In addition, the predominant cluster (A) was clonally related to that first described in Curitiba, in2003. In contrast, a single cluster of A. baumannii producing OXA-23 was found in São Luís city. Although there were previous reports regarding the spread of OXA-23-producing A. baumannii in Brazil the following features had not yet been assessed: i) the comparative analysis of OXA-23 producers genotypes originating in distinct and distant Brazilian cities, ii) the genetic location of the blaOXA-23 gene and iii) the presence of ISAba1 upstream blaOXA-23 probably resulting in high degree of carbapenem resistance. Thus, our study demonstrates that the clonal dissemination of OXA-23- producing A. baumannii had occurred in Brazil. These findings emphasize that infection control measures are urgently needed to reduce both the spread of multidrug resistantstrains and the number of infections caused by this pathogen. The second study refers to a case report involving a hospitalized patient that presented an wound infection due to OXA-72-producing A. baumannii. The referred clinical isolate, A 30235, was resistant to most antibiotics tested and showed reduced susctptibility to ampicillin/sulbactam. Successful transformation assays using A. baumannii ATCC 19606 as the recipient strain revealed the plasmid location of the blaOXA-72 gene. This plasmid showed molecular weight of about 86 Kb. We identified for the first time in Brazil, an A. baumannii clinical isolate producing OXA-72. The presence of this resistance determinant encoded by a gene located in a mobile genetic elemet, points out to an increasing diversity of OXA-type carbapenemase in Brazil with potential spread. Appropriate control measures should be taken to prevent the spread of OXA-72 producers among Brazilian hospitals, which it we have experienced with OXA-23- producing-A. baumannii in this country.
TEDE
BV UNIFESP: Teses e dissertações

Dissertação para obtenção do Grau de Mestre em Conservação e Restauro
É frequente, em peças de cerâmica arqueológica, o aparecimento de fenómenos de desagregação. Para a conservação deste material há a necessidade de aplicação de produtos que aumentem a sua coesão e resistência mecânica. Dado existirem poucos trabalhos científicos sobre o desempenho de consolidantes alternativos, a utilizar em cerâmica arqueológica, sentiu-se a necessidade de desenvolver um trabalho laboratorial sobre essa temática. Assim, efectuou-se o estudo comparativo de avaliação da eficácia inicial de dois produtos comerciais, o Tegovakon V® e o Paraloid B-72®, como consolidantes. Foram utilizados provetes (sãos) em cerâmica vermelha cozida a 980 °C. Os consolidantes foram aplicados por imersão total e por pincelagem. A cerâmica foi caracterizada por DRX e os consolidantes por FTIR. A micro-estrutura da cerâmica foi caracterizada, antes e após a consolidação, por MEV-EDE, PM e μ-CT. A eficácia inicial do processo de consolidação foi avaliada através de uma série de ensaios laboratoriais, nomeadamente: porosidade acessível à água, absorção de água por capilaridade, velocidade de propagação de ondas longitudinais, resistência à compressão pontual, ângulo de contacto, tempo de absorção da micro-gota e caracterização colorimétrica. A consolidação é um procedimento que interfere nas propriedades físicas e comportamentais da cerâmica intervencionada. Essas alterações nas propriedades da cerâmica são função do consolidante e do método de aplicação. Nos provetes consolidados, por imersão, com Tegovakon V® verificou-se a redução de 62% da porosidade e 98% da capilaridade, o aumento de 4,8% da resistência mecânica e a alteração de ΔΕ de aproximadamente 7,7. Nos provetes consolidados, por imersão, com Paraloid B-72® verificou-se a redução de 4,5% da porosidade e 87% da capilaridade e o aumento de 4,5% da resistência mecânica. A alteração de ΔΕ, aproximadamente 12, foi superior nos provetes consolidados por pincelagem. Com base nestes resultados podemos afirmar que o consolidante que apresenta melhor desempenho, no que diz respeito à eficácia inicial, é o Tegovakon V® aplicado por imersão.

Avec l’arrivée de l’Analyse IsoGéométrique (IGA), le calcul de coque est devenu possible en utilisant la géométrie exacte pour des maillages grossiers. Pour cela, les polynômes de Lagrange sont remplacés pour l’interpolation par des fonctions NURBS (technologie la plus courante en conception assistée par ordinateur). De plus, ces fonctions possèdent une continuité supérieure ce qui offre une meilleure précision qu’un calcul éléments finis à nombre de degrés de liberté égal. L’IGA a déjà été développée pour les formulations coques. Elle n’a été cependant que très peu étudiée pour les modèles massifs coque. Pourtant, cette deuxième approche est très utilisée par l’ingénieur car elle permet de calculer des structures minces à l’aide d’éléments continus 3D, c’est-à-dire en faisant intervenir uniquement des inconnues en déplacements. La difficulté en calcul de coque est de faire face au verrouillage qui conduit à une forte dégradation de la convergence de la solution. Le cadre NURBS ne permet pas lui-même de résoudre ce problème. La meilleure efficacité de l’approximation NURBS ne peut donc être atteinte sans le développement de techniques particulières pour supprimer le verrouillage. C’est le but de cette thèse dans le cadre des éléments massifs coque. Le premier travail a consisté, sur un problème de poutre courbe, à étendre les méthodes sans verrouillage habituelles au contexte NURBS. Deux nouvelles stratégies ont alors été développées pour les NURBS : la première est basée sur une technique d’intégration réduite tandis que la seconde fait appel à une projection B-bar. Le formalisme général des méthodes B-bar semblant plus adapté, c’est celui-ci que nous avons développé ensuite pour les éléments massifs coque. Plus précisément, nous avons mis en place une formulation mixte de laquelle nous avons pu dériver la projection B-bar équivalente. Cette démarche constitue d’un point de vue théorique le résultat principal du travail : une méthode systématique pour construire une projection B-bar consistante est de passer par une formulation mixte. D’un point de vue mise en œuvre, l’idée principale pour traiter le verrouillage des éléments massifs coque a été de modifier l’interpolation de la moyenne dans l’épaisseur de la coque des composantes du tenseur des contraintes. Un contrôle de hourglass a aussi été ajouté pour stabiliser l’élément dans certaines situations. L’élément obtenu est de bonne qualité pour une interpolation de bas degrés et des maillages grossiers : la version quadratique semble plus précise que des éléments standards NURBS de degré 4. La méthode proposée conduit à une matrice de rigidité globale de petite taille mais pleine. Ce problème est inhérent aux NURBS. Il a pu être limité ici en utilisant une procédure de type moindres carrés locaux pour approcher la projection B-bar. Finalement, l’élément mixte a été étendu avec succès en non linéaire géométrique ce qui témoigne du potentiel de la méthode pour mener des simulations complexes
With the introduction of IsoGeometric Analysis (IGA), the calculation of shell has become possible using the exact geometry for coarse meshes. In order to that, Lagrange polynomials are replaced by NURBS functions, the most commonly used technology in Computer-Aided Design, to perform the analysis. In addition, NURBS functions have a higher order of continuity, which leads to higher per-degree-of-freedom accuracy of the shell solution than with classical Finite Elements Analysis (FEA). IGA has now been widely applied in shell formulations. Nevertheless, it has still rarely been studied in the context of solid-shell models. This second shell approach is, however, very useful for engineers, since it enables to calculate thin structures using 3D solid elements, i.e. involving only displacements as degrees of freedom. The difficulty in shell analysis is to deal with locking which highly deteriorates the convergence of the solution. The NURBS framework does not enable to solve the problem directly. Then, to really benefit from NURBS in shells, specific strategies need to be implemented to answer the locking issue. This is the goal of the thesis in the context of solid-shell elements. The first work has consisted, on a curved beam problem, in extending the locking-free methods usually encountered in FEA to the NURBS context. The study resulted in the development of two new strategies for NURBS: the first one is based on a selective reduced integration technique and the second one makes use of a B-bar projection. The global formalism offered by the B-bar method appearing more suitable for NURBS, it has then been investigated for solid-shell elements. More precisely, a mixed formulation has first been elaborated from which, it has been possible to derive the equivalent B-bar projection. From a theoretical point of view, this strategy constitutes the most important result of this work: a systematic method to construct a consistent B-bar projection is to write a mixed formulation. With regards to the implementation, the main idea to treat locking of the solid-shell elements has been to modify the average of the strain and stress components across the thickness of the shell. Hourglass control has also been added to stabilize the element in particular situations. The resulting element is of good quality for low order approximations and coarse meshes: the quadratic version seems to be more accurate than basic NURBS elements of order 4. The proposed method leads to a global stiffness matrix of small size but full. This problem is inherent to NURBS functions. It has been limited here by using a local least squares procedure to approach the B-bar projection. Finally, the mixed element has been successfully extended to geometric non-linearity which reflects the ability of the methodology to be used in complex simulations

Das HERA-$B$-Experiment am Deutschen Elektronen-Synchrotron (DESY) in Hamburg dient der Untersuchung der Physik von Teilchen, die $b$-Quarks enthalten. Der Schwerpunkt des Ex\-pe\-ri\-mentes liegt auf der Messung der CP-Verletzung im System der neutralen $B$-Mesonen. Es wird erwartet, dass die pr\"azise Bestimmung der CP-Asymmetrie im Zerfallskanal $B^0(\bar{B}^0)\to J/\psi K_S^0$ gro{\ss}en Einfluss auf die Weiterentwicklung des Standardmodells der Elementarteilchenphysik und g\"angiger kosmologischer Theorien haben wird. Das HERA-$B$-Experiment nutzt den Protonenstrahl des HERA-Ringes, um in Kollisionen mit einem feststehenden Target paarweise $B$-Hadronen zu erzeugen. Die Wechselwirkungen werden in einem Vorw\"artsspektrometer mit etwa 600.000 Auslesekan\"alen nachgewiesen. Aufgrund der relativ niedrigen Schwerpunktsenergie von 41.6\,GeV sind Ereignisse mit $b$-Quarks im Vergleich zu Wechselwirkungen mit leichteren Quarks um etwa sechs Gr\"o{\ss}enordnungen unterdr\"uckt. Die Selektion von Signalereignissen stellt daher eine besondere Herausforderung dar. Sie wird von einem vierstufigen Datennahme- und Triggerystem \"ubernommen, das die Ereignisrate von 10\,MHz auf etwa 20\,Hz reduziert. Neben speziell entwickelter Elektronik werden im Triggersystem mehrere hundert handels\"ubliche PCs eingesetzt. Die Computer sind in zwei so genannten PC-Farmen mit jeweils mehr als 200 Prozessoren angeordnet, die die Rechenkapazit\"at f\"ur Triggerentscheidungen und die prompte Analyse der Ereignisdaten zur Verf\"ugung stellen. Auf der einen Farm laufen schnelle Triggerprogramme mit einer Rechenzeit von etwa 1--100\,ms pro Ereignis ab. Die andere Farm rekonstruiert die Ereignisse online, bevor die Daten auf Band dauerhaft archiviert werden. Die pro Ereignis aufgewandte Rechenzeit liegt dabei im Bereich einiger Sekunden. Die vorliegende Arbeit behandelt zwei Themenkreise. Einerseits wird die technische Umsetzung der Trigger- und der Rekonstruktionsfarm beschrieben. Besonderes Augenmerk liegt dabei auf den Software-Systemen, die den Farmen erforderliche Kalibrationsdaten verf\"ugbar machen und die zentrale \"Uberwachung der Ergebnisse der ablaufenden Programme gestatten. Der Hauptteil der Arbeit besch\"aftigt sich mit Algorithmen f\"ur eine dritte Triggerstufe, die zus\"atzlich zu existierenden Programmen auf der Triggerfarm zum Einsatz kommen sollen. Der Zerfall $B^0(\bar{B}^0)\to J/\psi X$ hat eine sehr klare Signatur, wenn das $J/\psi$ in ein $e^+e^-$- oder $\mu^+\mu^-$-Paar zerf\"allt. Im Triggersystem wird nach einem Paar entgegengesetzt geladener Leptonen des gleichen Typs gesucht, deren invariante Masse der des $J/\psi$ entspricht und deren Spuren von einem gemeinsamen Vertex in der N\"ahe des Targets ausgehen. Es wird davon ausgegangen, dass die Ausnutzung aller kinematischen Zwangsbedingungen ausreicht, um diesen Zerfallskanal klar von Untergrundereignissen zu trennen. Die dritte Triggerstufe soll dagegen auf Signalprozesse mit weniger kinematischen Beschr\"ankungen angewandt werden. Solche Ereignisse entstehen zum Beispiel dann, wenn zwei in der Proton-Target-Kollision erzeugte $B$-Mesonen semileptonisch zerfallen. Das Triggersystem selektiert lediglich die beiden Leptonen, die aber hier nicht von einem gemeinsamen Vertex kommen. Die dritte Triggerstufe soll f\"ur derartige Zerfallstopologien innerhalb von 100\,ms pro Ereignis weitere Kriterien zur Unterscheidung von Signal- und Untergrundprozessen aus den Daten extrahieren. In der Arbeit wird anhand von Monte-Carlo-Studien untersucht, inwieweit die Daten des Silizium-Vertexdetektors des Experimentes zur Entscheidungsfindung einer dritten Triggerstufe beitragen k\"onnen. Dabei wird die Rekonstruktion von Spuren aus der Zerfallskaskade der $B$-Hadronen zus\"atzlich zu den von der vorhergehenden Triggerstufe selektierten Lep\-ton\-en an\-ge\-strebt. Mithilfe einer schnellen Mustererkennung f\"ur den Vertexdetektor wird gezeigt, dass das Auffinden aller Spuren und die Anwendung von Triggeralgorithmen innerhalb des vorgegebenen Zeitfensters von 100\,ms m\"oglich sind. Die Bestimmung der Spurparameter nahe der Targetregion macht von der Methode des Kalman-Filters Gebrauch, um der Vielfachstreuung im Detektormaterial Rechnung zu tragen. Dabei tritt das Problem auf, dass weder der Impuls der gefundenen Spuren bekannt ist, noch die Materialverteilung im Vertexdetektor aus Zeitgr\"unden in aller Strenge ber\"ucksichtigt werden kann. Durch geeignete N\"aherungen gelingt es, eine ausreichende Genauigkeit f\"ur die Spurparameter zu erreichen. Die aufgefundenen Teilchen bilden den Ausgangspunkt f\"ur Triggeralgorithmen. Hierbei wird untersucht, welche Methoden am besten geeignet sind, um Signal- und Unter\-grund\-ereignisse voneinander zu trennen. Es erweist sich, dass das Auffinden von Spuren mit gro{\ss}em Impaktparameter aussichtsreichere Ans\"atze als eine Suche nach Sekund\"arvertices bietet.
The HERA-$B$ experiment at Deutsches Elektronen-Synchrotron (DESY) Hamburg aims at investigating the physics of particles containing $b$ quarks. The experiment focusses on measuring CP violation in the system of neutral $B$ mesons. It is expected that the precise determination of the CP asymmetry in the channel $B^0(\bar{B}^0)\to J/\psi K_S^0$ will have an impact on the further development of the Standard Model of Elementary Particle Physics and cosmological theories. The HERA-$B$ experiment uses the proton beam of the HERA storage ring in fixed-target mode. $B$ hadrons are produced in pairs when protons from the beam halo interact with target nuclei. The interactions are recorded by a forward-spectrometer with roughly 600.000 readout channels. At the HERA-$B$ centre-of-mass energy of 42.6\,GeV, the $b\bar{b}$ cross section is only a tiny fraction of the total inelastic cross section. Only one in about 10$^6$ events contains $b$ quarks, which turns the selection of signal events into a particular challenge. The selection is accomplished by a four-stage data acquisition and trigger system reducing the event rate from 10\,MHz to about 20\,Hz. Besides custom-made electronics, several hundreds of PCs are used in the trigger system. The computers are arranged in two so-called PC farms with more than 200 processors each. The PC farms provide the computing capacity for trigger decisions and the prompt analysis of event data. One farm executes fast trigger programs with a computing time of 1--100\,ms per event. The other farm performs online reconstruction of the events before data are archived on tape. The computing time per event is in the range of several seconds. This thesis covers two topics. In the beginning, the technical implementation of the trigger and the reconstruction farm are described. In doing so, emphasis is put on the software systems which make calibration data available to the farms and which provide a centralised view on the results of the executing processes. The principal part of this thesis deals with algorithms for a Third Level Trigger. This trigger is to come into operation on the trigger farm together with existing programs. Processes of the type $B^0(\bar{B}^0)\to J/\psi X$ have a very clean signature when the $J/\psi$ decays to a $e^+e^-$ or $\mu^+\mu^-$ pair. The trigger system attempts to identify two unlike-sign leptons of the same flavour whose invariant mass matches the $J/\psi$. In later steps, the tracks are required to originate from a common vertex close to the target. It is assumed that these kinematic constraints are sufficient to pick out events of this type among the copious background processes. In contrast, the Third Level Trigger is to be applied to signal processes with fewer kinematic constraints. Such events occur for example when two $B$ mesons, which were created in a proton-target collision, decay semileptonically. The trigger system selects merely the two leptons which do not originate from a common vertex in this case. The Third Level Trigger has 100\,ms at its disposal to extract further criteria from the data which can serve to distinguish between signal and background events. This thesis investigates with the aid of Monte-Carlo simulations how the data of the experiment's silicon vertex detector can contribute to the decisions of a Third Level Trigger. The trigger aims at reconstructing tracks from the decay cascade of $B$ mesons in addition to the leptons selected by the preceding trigger levels. A fast pattern recognition for the vertex detector demonstrates that the reconstruction of all tracks and the application of trigger algorithms are possible within the given time slot of 100\,ms. The determination of track parameters in the target region exploits the Kalman-filter method to account for the multiple scattering of particles in the detector material. The application of this method is, however, made difficult by two facts. First, the momentum of the reconstructed tracks is not known. And, second, the material distribution in the detector cannot be taken into consideration in detail due to timing limitations. Adequate approximations for the momentum and the material traversed by a particle help to accomplish a sufficient accuracy of the track parameters. The reconstructed tracks constitute the starting point of several trigger algorithms, whose suitability to select signal events is investigated. Our studies indicate that the reconstruction of tracks with large impact parameters is a more promising approach than a search for secondary vertices.

The presence of TP53 mutations has been associated with inferior outcome in diffuse large B-cell lymphoma (DLBCL) and chronic lymphocytic leukemia (CLL). In DLBCL, the impact of the TP53 codon 72 polymorphism and MDM2 SNP309 has not been clearly elucidated, whereas MDM2 SNP309 was suggested as a poor-prognostic marker in CLL. In addition, p16INK4a promoter hypermethylation has been implicated as a negative prognostic factor in DLBCL. The aim of this thesis was to further evaluate these molecular markers in well-characterised materials of DLBCL and CLL. In paper I, we investigated the prognostic role of TP53 mutation, codon 72 polymorphism and MDM2 SNP309 in DLBCL (n=102). The presence of TP53 mutations (12.7%) correlated with a poor lymphoma-specific and progression-free survival, and a particularly pronounced effect was observed in the germinal center subtype. Neither the MDM2 SNP309 nor the TP53 codon 72 polymorphism had an impact on age of onset or survival. In paper II, we applied pyrosequencing to measure the level of p16INK4a methylation in DLBCL (n=113). Thirty-seven percent of cases displayed p16INK4a methylation; however, no clear association could be observed between degree of methylation and clinical characteristics or lymphoma-specific survival. In papers III–IV, we investigated the prognostic role of MDM2 SNP309 (n=418) and TP53 mutation (n=268) in CLL. No correlation was observed between any particular MDM2 SNP309 genotype and time to treatment and overall survival. Furthermore, no association was found between the different MDM2 SNP309 genotypes and established CLL prognostic markers. TP53 mutations were detected in 3.7% of CLL patients; where the majority showed a concomitant 17p-deletion and only three carried TP53 mutations without 17p-deletion. We confirmed a significantly shorter overall survival and time to treatment in patients with both TP53 mutation and 17p-deletion. Altogether, our studies could confirm the negative prognostic impact of TP53 mutations in DLBCL, whereas MDM2 SNP309 and TP53 codon 72 polymorphisms appear to lack clinical relevance. We also question the role of p16INKa methylation as a poor-prognostic factor in DLBCL. Finally, the presence of TP53 mutation in CLL appears to be rare at disease onset and instead arise during disease progression.

Ce travail de thèse est axé sur le domaine du traitement d'images biomédicales. L'objectif de notre étude est l'estimation des paramètres traduisant les propriétés mécaniques de l'artère carotide in vivo en imagerie échographique, dans une optique de détection précoce de la pathologie cardiovasculaire. L'analyse du mouvement longitudinal des tissus de la paroi artérielle, i.e. dans la même direction que le flux sanguin, représente la motivation majeure de ce travail. Les trois contributions principales proposées dans ce travail sont i) le développement d'un cadre méthodologique original et semi-automatique, dédié à la segmentation et à l'estimation du mouvement de la paroi artérielle dans des séquences in vivo d'images ultrasonores mode-B, ii) la description d'un protocole de génération d'une référence, faisant intervenir les opérations manuelles de plusieurs experts, dans le but de quantifier la précision des résultats de notre méthode malgré l'absence de vérité terrain inhérente à la modalité échographique, et iii) l'évaluation clinique de l'association entre les paramètres mécaniques et dynamiques de la paroi carotidienne et les facteurs de risque cardiovasculaire dans le cadre de la détection précoce de l'athérosclérose. Nous proposons une méthode semi-automatique, basée sur une approche conjointe de segmentation des contours de la paroi et d'estimation du mouvement des tissus. L'extraction de la position des interfaces est réalisée via une approche spécifique à la structure morphologique de la carotide, basée sur une stratégie de programmation dynamique exploitant un filtrage adapté. L'estimation du mouvement est réalisée via une méthode robuste de mise en correspondance de blocs (block matching), basée sur la connaissance du déplacement a priori ainsi que sur la mise à jour temporelle du bloc de référence par un filtre de Kalman spécifique. La précision de notre méthode, évaluée in vivo, correspond au même ordre de grandeur que celle résultant des opérations manuelles réalisées par des experts, et reste sensiblement meilleure que celle obtenue avec deux autres méthodes traditionnelles (i.e. une implémentation classique de la technique de block matching et le logiciel commercial Velocity Vector Imaging). Nous présentons également quatre études cliniques réalisées en milieu hospitalier, où nous évaluons l'association entre le mouvement longitudinal et les facteurs de risque cardiovasculaire. Nous suggérons que le mouvement longitudinal, qui représente un marqueur de risque émergent et encore peu étudié, constitue un indice pertinent et complémentaire aux marqueurs traditionnels dans la caractérisation de la physiopathologie artérielle, reflète le niveau de risque cardiovasculaire global, et pourrait être bien adapté à la détection précoce de l'athérosclérose.

A fotografia teve o importante contributo dos negativos em gelatina e brometo de prata com suporte em vidro durante o período compreendido entre 1878 e 1925, constituindo grande parte das coleções de arquivos fotográficos. O impacto das degradações nestas peças históricas repercute-se não só ao nível da preservação da peça mas também dos seus conteúdos. Nos casos em que se realiza uma intervenção de restauro utilizam-se adesivos referênciados para peças em vidro, sem estudos sobre as interações químicas que podem ocorrer entre os materiais. Tendo presente a fragilidade do material, este estudo tem como objetivo contribuir para a conservação ativa destas espécies fotográficas desenvolvendo um método sustentado por estudos sobre a interação dos adesivos utilizados com os materiais constituintes dos negativos (vidro, sais de prata e gelatina), que permita uma intervenção fundamentada e um maior acesso às peças. O trabalho iniciou-se com o estudo de quatro adesivos indicados para estabilização destas espécies fotográficas: gelatina (colagénio), Paraloid™ B-72, Hxtal® NYL-1 e Araldite® 2020. Foram preparadas amostras, nas quais se aplicaram adesivos, tendo sido submetidas a ensaios de envelhecimento artificial. Os resultados foram acompanhados através de Microscopia Ótica (MO) e análise molecular por micro-espectroscopia de infravermelho com transformada de Fourier (μ-FTIR), e foram realizados testes mecânicos de tração. Em questões de reversibilidade, apenas as uniões com Paraloid™ B-72 mostraram reversibilidade da união, sendo que relativamente à degradação deste adesivo com os diferentes solventes, foi percetível uma maior alteração do Paraloid™ B-72 com acetona, do que com xileno. No que diz respeito aos restantes adesivos, a gelatina, para além de interferir visualmente com a emulsão, apresenta um poder de adesão muito baixo. Já as resinas epoxídicas, com uma metodologia mais trabalhada poderão apresentar resultados de união satisfatórios. No entanto provocam facilmente excessos sobre a emulsão, danificando-a. Foi ainda estudada uma coleção pertencente ao Arquivo Histórico Ultramarino-Instituto de Investigação Científica Tropical (AHU-IICT), que apresenta casos com degradação ao nível da emulsão, nomeadamente o destacamento da emulsão, tendo sido realizadas análises elementar e molecular por micro-fluorescência de raios-X dispersiva de energias (μ-EDXRF) e μ-FTIR, respetivamente.