Academic literature on the topic 'Avocado Sunblotch'

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Journal articles on the topic "Avocado Sunblotch"

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Saucedo Carabez, José Ramón, Daniel Téliz Ortiz, Moisés Roberto Vallejo Pérez, and Hugo Beltrán Peña. "The Avocado Sunblotch Viroid: An Invisible Foe of Avocado." Viruses 11, no. 6 (May 29, 2019): 491. http://dx.doi.org/10.3390/v11060491.

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This review collects information about the history of avocado and the economically important disease, avocado sunblotch, caused by the avocado sunblotch viroid (ASBVd). Sunblotch symptoms are variable, but the most common in fruits are irregular sunken areas of white, yellow, or reddish color. On severely affected fruits, the sunken areas may become necrotic. ASBVd (type species Avocado sunblotch viroid, family Avsunviroidae) replicates and accumulates in the chloroplast, and it is the smallest plant pathogen. This pathogen is a circular single-stranded RNA of 246–251 nucleotides. ASBVd has a restricted host range and only few plant species of the family Lauraceae have been confirmed experimentally as additional hosts. The most reliable method to detect ASBVd in the field is to identify symptomatic fruits, complemented in the laboratory with reliable and sensitive molecular techniques to identify infected but asymptomatic trees. This pathogen is widely distributed in most avocado-producing areas and causes significant reductions in yield and fruit quality. Infected asymptomatic trees play an important role in the epidemiology of this disease, and avocado nurseries need to be certified to ensure they provide pathogen-free avocado material. Although there is no cure for infected trees, sanitation practices may have a significant impact on avoiding the spread of this pathogen.
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Marcos, J. F., and R. Flores. "Subcellular location of avocado sunblotch viroid in avocado leaves." Plant Science 67, no. 2 (January 1990): 237–44. http://dx.doi.org/10.1016/0168-9452(90)90248-m.

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Pallas, Vicente, Isabel García-Luque, Esteban Domingo, and Ricardo Flores. "Sequence variability in avocado sunblotch viroid (ASBV)." Nucleic Acids Research 16, no. 20 (1988): 9864. http://dx.doi.org/10.1093/nar/16.20.9864.

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De La Torre-A, R., D. Téliz-Ortiz, V. Pallás, and J. A. Sánchez-Navarro. "First Report of Avocado sunblotch viroid in Avocado from Michoacán, México." Plant Disease 93, no. 2 (February 2009): 202. http://dx.doi.org/10.1094/pdis-93-2-0202b.

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The State of Michoacán, México cultivates approximately 100,000 ha of avocados (Persea americana M.) (4). During a survey from 2006 to 2007 in cv. Hass avocado groves in Tingambato County, in the State of Michoacán, deep yellow spots and streaks, which sometimes became necrotic or reddish, were observed on the skin of fruits and the pulp of the fruit also showed big yellow spots. Some young shoots developed fine, yellow streaks, and leaves of symptomatic trees sometimes showed irregular, white-to-yellow spots. These symptoms were similar to those recorded for Avocado sunblotch viroid (ASBVd) (3). To determine if ABSVd was associated with these symptoms, total RNA extracted (1) from the skin and pulp of symptomatic and asymptomatic fruits and also from leaves and bark of shoots from five trees collected in a commercial plot in Tingambato County was tested by a one-step reverse transcription (RT)-PCR protocol using one primer pair to amplify specifically the complete ASBVd genome sequence (3). All 30 samples of skin and pulp of fruits, leaves, and cortex of shoots from symptomatic trees yielded two PCR fragments with estimated sizes of 250 and 500 base pairs (bp) corresponding to the putative monomeric and dimeric forms of ASBVd, respectively. The 500-bp RT-PCR fragments obtained from the different samples were purified from an agarose gel and cloned. The 249-bp nucleotide sequence of the ASBVd genomic monomer was determined using the clones from the fruit skin from sample Arb No. 3 (GenBank Accession No. EU888588), pulp from sample Arb No. 5 (GenBank Accession No. EU888590), leaves from samples Arb No. 15 (GenBank Accession No. EU888589) and Arb No. 8 (GenBank Accession Nos. EU888591 and EU888592), and cortex of shoots from sample Arb No. 16 (GenBank Accession Nos. EU888593, EU888594, EU888595, EU888596, and EU888597). BLAST analysis of the ASBVd sequences showed a range of 98 to 100% nucleotide identity to ASBVd sequences (GenBank Accession Nos. AF404064, AF404051, or AF229821). A clone of the Michoacán ASBVd (GenBank Accession No. EU888593) was used to synthesize a Dig-High Prime-UTP-T7 (Roche, Mannheim, Germany) fluorescent riboprobe complementary to the ASBVd plus strand to perform a dot-blot analysis as described previously (2). All ASBVd samples positive by RT-PCR gave a strong signal in the dot-blot analysis. This riboprobe will be used to index the ASBVd in other commercial avocado groves in Michoacán. To our knowledge, this is the first report of ASBVd in Michoacán, México. References: (1) D. J. Mackenzie et al. Plant Dis. 81:222, 1997. (2) J. A. Sánchez-Navarro et al. Plant Pathol. 47:780, 1998. (3) R. J. Schnell et al. Plant Dis. 81:1023, 1997. (4) D. Téliz and A. Mora. El aguacate y su Manejo Integrado. Mundiprensa, Mexico City, 2007.
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Schnell, Raymond John, Cecile Lorraine Tondo, David Norton Kuhn, Michael Carl Winterstein, Tomas Ayala-Silva, and John Michael Moore. "Spatial Analysis of Avocado Sunblotch Disease in an Avocado Germplasm Collection." Journal of Phytopathology 159, no. 11-12 (September 19, 2011): 773–81. http://dx.doi.org/10.1111/j.1439-0434.2011.01838.x.

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Schnell, R. J., D. N. Kuhn, C. T. Olano, and W. E. Quintanilla. "Sequence Diversity among Avocado Sunblotch Viroids Isolated from Single Avocado Trees." Phytoparasitica 29, no. 5 (October 2001): 451–60. http://dx.doi.org/10.1007/bf02981864.

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Saucedo-Carabez, J. R., D. Téliz-Ortiz, S. Ochoa-Ascencio, D. Ochoa-Martínez, M. R. Vallejo-Pérez, and H. Beltrán-Peña. "Effect of Avocado sunblotch viroid (ASBVd) on avocado yield in Michoacan, Mexico." European Journal of Plant Pathology 138, no. 4 (December 21, 2013): 799–805. http://dx.doi.org/10.1007/s10658-013-0354-9.

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Schnell, R. J., D. N. Kuhn, C. M. Ronning, and D. Harkins. "Application of RT-PCR for Indexing Avocado Sunblotch Viroid." Plant Disease 81, no. 9 (September 1997): 1023–26. http://dx.doi.org/10.1094/pdis.1997.81.9.1023.

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A method for the routine detection of avocado sunblotch viroid (ASBVd) in nucleic acid extracts of infected avocado tissues by reverse transcription-polymerase chain reaction (RT-PCR) was developed using ASBVd-specific primers. Amplified cDNA products were analyzed by electrophoresis on nondenaturing 6% polyacrylamide slab gels. The size of the major RT-PCR product from ASBVd-infected tissue was estimated to be 250 bp. This product was absent from amplified extracts of uninfected tissue. The amplification product from ASBVd was sequenced by the dideoxynucleotide chain termination method, and the sequence was over 97% identical to the published sequence. The RT-PCR assay is sensitive enough to allow viroid detection without requiring large amounts of tissue, highly purified ASBVd, or molecular hybridization.
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Rakowski, Andrew G., and Robert H. Symons. "Comparative sequence studies of variants of avocado sunblotch viroid." Virology 173, no. 1 (November 1989): 352–56. http://dx.doi.org/10.1016/0042-6822(89)90256-0.

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Everett, Kerry R., and Brad Siebert. "Exotic plant disease threats to the New Zealand avocado industry and climatic suitability: a review." New Zealand Plant Protection 71 (July 28, 2018): 25–38. http://dx.doi.org/10.30843/nzpp.2018.71.140.

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The avocado industry was established in New Zealand from several importations dating back to 1907. Several serious pathogens found elsewhere in the world were not imported. A literature review and internet search were conducted to determine what serious avocado pathogens are not present in New Zealand and the potential impact they could have if they established. Relevant information was summarised for six pathogens determined to be the most serious of avocado and not known to be present in New Zealand: avocado sunblotch viroid (ASBVd); Pseudocercospora purpurea (cercospora spot); Raffaelea lauricola (laurel wilt); Fusarium sp. (fusarium dieback); Phellinus noxius (brown root rot); and Sphaceloma perseae (avocado scab). Laurel wilt, brown root rot, cercospora spot and fusarium dieback could become established in New Zealand if the climate here becomes warmer but establishment of ASBVd and avocado scab (which are not restricted to hot climates) is more likely.
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Dissertations / Theses on the topic "Avocado Sunblotch"

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Delan-Forino, Clémentine. "Le Viroïde Avocado sunblotch : étude de sa réplication dans la levure Saccharomyces cerevisiae et de sa structure." Paris 6, 2011. http://www.theses.fr/2011PA066267.

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Les viroïdes sont les plus petits agents pathogènes connus (246 à 401 nt). Ce sont des ARN nus, simple-brin, circulaires et non-codants dont les deux séquences complémentaires nommées (+) et (-) co-existent dans les cellules. Il existe deux familles : les Pospiviroidae et les Avsunviroidae. Ces derniers ont dans la séquence de chaque polarité un ribozyme en tête de marteau, indispensable à leur réplication ARN dépendante. À ce jour, tous les viroïdes ont été identifiés chez des végétaux supérieurs. Le premier objectif de ma thèse a été de tester la réplication d’un Avsunviroidae, le viroïde Avocado sunblotch (ASBVd), dans un système modèle non-photosynthétique, la levure Saccharomyces cerevisiae. J’ai démontré que l’ASBVd est capable de se répliquer et de se maintenir pendant au moins 25 générations dans la levure. De plus, l’ASBVd est sensible à la dégradation des ARN nucléaire et cytoplasmique de S. Cerevisiae. Les interactions des viroïdes avec les facteurs cellulaires semblent intimement liés à leurs caractéristiques structurales et catalytiques. Un très haut degré de complémentarité entre les différentes régions de ces ARN leur permet d’adopter des structures complexes. Le deuxième objectif de ma thèse a été d’étudier le comportement cinétique et structural des brins (+) et (-). J’ai mis en évidence des différences de propriétés biophysiques entre les deux brins et une plus grande efficacité d’auto-clivage de l’ASBVd (-). La structure de l’ASBVd est déterminée par une technique biochimique innovante et haut débit, le SHAPE (sélective 2’-hydroxyl acylation analysed by primer extension), pour préciser et localiser les différences structurales dans deux polarités
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TALINI, GIULIA. "Studies on catalytic RNA molecules relevant to the RNA wolrd hypothesis." Doctoral thesis, 2009. http://hdl.handle.net/2158/546268.

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Books on the topic "Avocado Sunblotch"

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n, Isidro Elias Suarez Padro. Avocado sunblotch viroid (ASBVd) in cell and tissue cultures of avocado (Persea americana Mill.). 2003.

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Book chapters on the topic "Avocado Sunblotch"

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Desjardins, Paul R. "Avocado Sunblotch." In The Viroids, 299–313. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4613-1855-2_18.

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Mathews, Deborah M., Sohrab Bodaghi, James A. Heick, and J. Allan Dodds. "Detection of Avocado Sunblotch and Other Viroids Using RNA Filter Paper Capture and RT-PCR." In Methods in Molecular Biology, 219–33. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1464-8_19.

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Kuhn, David N., Andrew D. W. Geering, and Jonathan Dixon. "Avocado Sunblotch Viroid." In Viroids and Satellites, 297–305. Elsevier, 2017. http://dx.doi.org/10.1016/b978-0-12-801498-1.00028-0.

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