Relevant bibliographies by topics / Automated patch clamp

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  4. Conference papers

Academic literature on the topic 'Automated patch clamp'

Author: Grafiati
Published: 14 March 2023

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Journal articles on the topic "Automated patch clamp"

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Shieh, Char-Chang. "Automated high-throughput patch-clamp techniques." Drug Discovery Today 9, no. 13 (July 2004): 551–52. http://dx.doi.org/10.1016/s1359-6446(04)03113-7.

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Annecchino, Luca A., and Simon R. Schultz. "Progress in automating patch clamp cellular physiology." Brain and Neuroscience Advances 2 (January 1, 2018): 239821281877656. http://dx.doi.org/10.1177/2398212818776561.

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Patch clamp electrophysiology has transformed research in the life sciences over the last few decades. Since their inception, automatic patch clamp platforms have evolved considerably, demonstrating the capability to address both voltage- and ligand-gated channels, and showing the potential to play a pivotal role in drug discovery and biomedical research. Unfortunately, the cell suspension assays to which early systems were limited cannot recreate biologically relevant cellular environments, or capture higher order aspects of synaptic physiology and network dynamics. In vivo patch clamp electrophysiology has the potential to yield more biologically complex information and be especially useful in reverse engineering the molecular and cellular mechanisms of single-cell and network neuronal computation, while capturing important aspects of human disease mechanisms and possible therapeutic strategies. Unfortunately, it is a difficult procedure with a steep learning curve, which has restricted dissemination of the technique. Luckily, in vivo patch clamp electrophysiology seems particularly amenable to robotic automation. In this review, we document the development of automated patch clamp technology, from early systems based on multi-well plates through to automated planar-array platforms, and modern robotic platforms capable of performing two-photon targeted whole-cell electrophysiological recordings in vivo.
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Haythornthwaite, Alison, Andrea Brueggemann, Cecilia Farre, Sonja Stoelzle, Claudia Haarmann, Michael George, and Niels Fertig. "Automated patch clamp for hERG safety screening." Journal of Pharmacological and Toxicological Methods 60, no. 2 (September 2009): 222. http://dx.doi.org/10.1016/j.vascn.2009.04.065.

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Jacobsen, Rasmus B., and Naja Møller M. Sørensen. "CFTR on an Automated Patch Clamp System." Biophysical Journal 110, no. 3 (February 2016): 452a. http://dx.doi.org/10.1016/j.bpj.2015.11.2426.

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Holst, Gregory L., William Stoy, Bo Yang, Ilya Kolb, Suhasa B. Kodandaramaiah, Lu Li, Ulf Knoblich, et al. "Autonomous patch-clamp robot for functional characterization of neurons in vivo: development and application to mouse visual cortex." Journal of Neurophysiology 121, no. 6 (June 1, 2019): 2341–57. http://dx.doi.org/10.1152/jn.00738.2018.

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Patch clamping is the gold standard measurement technique for cell-type characterization in vivo, but it has low throughput, is difficult to scale, and requires highly skilled operation. We developed an autonomous robot that can acquire multiple consecutive patch-clamp recordings in vivo. In practice, 40 pipettes loaded into a carousel are sequentially filled and inserted into the brain, localized to a cell, used for patch clamping, and disposed. Automated visual stimulation and electrophysiology software enables functional cell-type classification of whole cell-patched cells, as we show for 37 cells in the anesthetized mouse in visual cortex (V1) layer 5. We achieved 9% yield, with 5.3 min per attempt over hundreds of trials. The highly variable and low-yield nature of in vivo patch-clamp recordings will benefit from such a standardized, automated, quantitative approach, allowing development of optimal algorithms and enabling scaling required for large-scale studies and integration with complementary techniques. NEW & NOTEWORTHY In vivo patch-clamp is the gold standard for intracellular recordings, but it is a very manual and highly skilled technique. The robot in this work demonstrates the most automated in vivo patch-clamp experiment to date, by enabling production of multiple, serial intracellular recordings without human intervention. The robot automates pipette filling, wire threading, pipette positioning, neuron hunting, break-in, delivering sensory stimulus, and recording quality control, enabling in vivo cell-type characterization.
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Bruhn, Brandon R., Haiyan Liu, Stefan Schuhladen, Alan J. Hunt, Aghapi Mordovanakis, and Michael Mayer. "Dual-pore glass chips for cell-attached single-channel recordings." Lab Chip 14, no. 14 (2014): 2410–17. http://dx.doi.org/10.1039/c4lc00370e.

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Wu (吴秋雨), Qiuyu, Ilya Kolb, Brendan M. Callahan, Zhaolun Su, William Stoy, Suhasa B. Kodandaramaiah, Rachael Neve, et al. "Integration of autopatching with automated pipette and cell detection in vitro." Journal of Neurophysiology 116, no. 4 (October 1, 2016): 1564–78. http://dx.doi.org/10.1152/jn.00386.2016.

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Patch clamp is the main technique for measuring electrical properties of individual cells. Since its discovery in 1976 by Neher and Sakmann, patch clamp has been instrumental in broadening our understanding of the fundamental properties of ion channels and synapses in neurons. The conventional patch-clamp method requires manual, precise positioning of a glass micropipette against the cell membrane of a visually identified target neuron. Subsequently, a tight “gigaseal” connection between the pipette and the cell membrane is established, and suction is applied to establish the whole cell patch configuration to perform electrophysiological recordings. This procedure is repeated manually for each individual cell, making it labor intensive and time consuming. In this article we describe the development of a new automatic patch-clamp system for brain slices, which integrates all steps of the patch-clamp process: image acquisition through a microscope, computer vision-based identification of a patch pipette and fluorescently labeled neurons, micromanipulator control, and automated patching. We validated our system in brain slices from wild-type and transgenic mice expressing channelrhodopsin 2 under the Thy1 promoter (line 18) or injected with a herpes simplex virus-expressing archaerhodopsin, ArchT. Our computer vision-based algorithm makes the fluorescent cell detection and targeting user independent. Compared with manual patching, our system is superior in both success rate and average trial duration. It provides more reliable trial-to-trial control of the patching process and improves reproducibility of experiments.
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Harrison, Reid R., Ilya Kolb, Suhasa B. Kodandaramaiah, Alexander A. Chubykin, Aimei Yang, Mark F. Bear, Edward S. Boyden, and Craig R. Forest. "Microchip amplifier for in vitro, in vivo, and automated whole cell patch-clamp recording." Journal of Neurophysiology 113, no. 4 (February 15, 2015): 1275–82. http://dx.doi.org/10.1152/jn.00629.2014.

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Patch clamping is a gold-standard electrophysiology technique that has the temporal resolution and signal-to-noise ratio capable of reporting single ion channel currents, as well as electrical activity of excitable single cells. Despite its usefulness and decades of development, the amplifiers required for patch clamping are expensive and bulky. This has limited the scalability and throughput of patch clamping for single-ion channel and single-cell analyses. In this work, we have developed a custom patch-clamp amplifier microchip that can be fabricated using standard commercial silicon processes capable of performing both voltage- and current-clamp measurements. A key innovation is the use of nonlinear feedback elements in the voltage-clamp amplifier circuit to convert measured currents into logarithmically encoded voltages, thereby eliminating the need for large high-valued resistors, a factor that has limited previous attempts at integration. Benchtop characterization of the chip shows low levels of current noise [1.1 pA root mean square (rms) over 5 kHz] during voltage-clamp measurements and low levels of voltage noise (8.2 μV rms over 10 kHz) during current-clamp measurements. We demonstrate the ability of the chip to perform both current- and voltage-clamp measurement in vitro in HEK293FT cells and cultured neurons. We also demonstrate its ability to perform in vivo recordings as part of a robotic patch-clamping system. The performance of the patch-clamp amplifier microchip compares favorably with much larger commercial instrumentation, enabling benchtop commoditization, miniaturization, and scalable patch-clamp instrumentation.
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P.G. Korsgaard, Mads. "Voltage- and Current Clamp on Induced Pluripotent Cardiomyocytes with Automated Patch Clamp." Biophysical Journal 112, no. 3 (February 2017): 414a. http://dx.doi.org/10.1016/j.bpj.2016.11.2218.

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Brüggemann, Andrea, Claudia Haarmann, Markus Rapedius, Tom Goetze, Ilka Rinke, Michael George, and Niels Fertig. "Characterization of iPS Derived Cardiomyocytes in Voltage Clamp and Current Clamp by Automated Patch Clamp." Biophysical Journal 112, no. 3 (February 2017): 236a. http://dx.doi.org/10.1016/j.bpj.2016.11.1290.

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Dissertations / Theses on the topic "Automated patch clamp"

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Farhad, Jahanfar. "Identifying antagonist drugs for TRPM8 ion channel as candidates for repurposing." Doctoral thesis, Università di Siena, 2021. http://hdl.handle.net/11365/1162721.

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Even though it is confirmed that ion channels are at the centre of many diseases, approved drugs are only available for small percentage of these proteins, and yet many pathologically important ion channels like transient receptor potential (TRP) cation channels remain without approved drugs. One reason could be the time-consuming and expensive process in drug discovery. Which has high possibility of failure in any step even after approval and marketing. Therefore, repurposing approved drugs might be considered as a solution and may offer an accelerated procedure in finding new treatments for patients. For the present research we selected TRPM8 ion channel as a neglected target despite growing number of studies regarding its association with numerous diseases. In this project we have first identified potent antagonists for TRPM8 ion channel among approved drugs, by using mainly the automated patch clamp device IonFlux 16. Such device allowed us to screen blocking potency of drugs against TRPM8 ion channel in time efficient way. Our approach consisted of using ligand-based virtual screening method, to optimize our screening by identifying candidates for further screening. We also studied possible interactions of identified drugs with antagonist binding site on TRPM8 channel by molecular docking. Furthermore, we have evaluated the effects of identified antagonists against different types of pancreatic ductal adenocarcinoma (PDAC) cells. We were able to identify four drugs with IC50 lower than 50 µM including propranolol, propafenone, carvedilol and nebivolol. Among them nebivolol with IC50 = 0.97± 0.15 µM and carvedilol with IC50 = 9.1 ± 0.6 µM were the most potent blockers. Studying the interactions of identified drugs with known binding site of TRPM8 by molecular docking, revealed high possibility of direct binding of nebivolol to binding site of TRPM8. Nebivolol was the most cytotoxic drug against PDACs, but it was also toxic against non-cancerous HEK-293 cells. While carvedilol had cytotoxic against PDACs, interestingly it wasn’t cytotoxic against HEK-293 cells. Result of these study will provide promising candidates for drug repurposing and will propose promising lead compound in drug discovery for new antagonists of TRPM8 ion channel. Also, our method of approach for identifying candidate drugs as agonist or antagonist could be applied for other ion channels.
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Book chapters on the topic "Automated patch clamp"

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Milligan, Carol J., and Svenja Pachernegg. "Utilising Automated Electrophysiological Platforms in Epilepsy Research." In Patch Clamp Electrophysiology, 133–55. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_7.

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Boddum, Kim, Peder Skafte-Pedersen, Jean-Francois Rolland, and Sandra Wilson. "Optogenetics and Optical Tools in Automated Patch Clamping." In Patch Clamp Electrophysiology, 311–30. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_16.

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Milligan, Carol J., and Clemens Möller. "Automated Planar Patch-Clamp." In Methods in Molecular Biology, 171–87. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-351-0_13.

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Rosholm, Kadla R., Kim Boddum, and Anders Lindquist. "Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology." In Patch Clamp Electrophysiology, 93–108. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_5.

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Prime, Rebecca, Rachel Brown, Juha Kammonen, Anthony J. Kirkup, and Edward Stevens. "Automated Patch Clamp: Advantages and Limitations." In Encyclopedia of Biophysics, 141–47. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-16712-6_373.

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Fejtl, Michael, Uwe Czubayko, Alexander Hümmer, Tobias Krauter, and Albrecht Lepple-Wienhues. "Automated Glass Pipette-Based Patch-Clamp Techniques." In Neuromethods, 435–50. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-492-6_15.

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Milligan, Carol J., and Lin-Hua Jiang. "Automated Planar Patch-Clamp Recording of P2X Receptors." In Methods in Molecular Biology, 285–300. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9717-6_21.

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Sunesen, Morten, and Rasmus B. Jacobsen. "Study of TRP Channels by Automated Patch Clamp Systems." In Transient Receptor Potential Channels, 107–23. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-94-007-0265-3_5.

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Houtmann, Sylvie, Brigitte Schombert, Camille Sanson, Michel Partiseti, and G. Andrees Bohme. "Automated Patch-Clamp Methods for the hERG Cardiac Potassium Channel." In Methods in Molecular Biology, 187–99. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7172-5_10.

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Obergrussberger, Alison, Claudia Haarmann, Sonja Stölzle-Feix, Nadine Becker, Atsushi Ohtsuki, Andrea Brüggemann, Michael George, and Niels Fertig. "Automated Patch Clamp Recordings of Human Stem Cell-Derived Cardiomyocytes." In Methods in Pharmacology and Toxicology, 57–82. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6661-5_4.

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Conference papers on the topic "Automated patch clamp"

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Yang, Runhuai, King W. C. Lai, Ning Xi, and Jie Yang. "Development of automated patch clamp system for electrophysiology." In 2013 IEEE International Conference on Robotics and Biomimetics (ROBIO). IEEE, 2013. http://dx.doi.org/10.1109/robio.2013.6739793.

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Yang, Shengjie, and King Wai Chiu Lai. "A Predictive Model of Seal Condition in Automated Patch Clamp System." In 2022 International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS). IEEE, 2022. http://dx.doi.org/10.1109/marss55884.2022.9870494.

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Zhipeng, Ding, Patthara Kongsuphol, Teh Poh Giao, and Zhang Qingxin. "Lateral micro fluidic channels array chip fabrication for automated patch clamp application." In 2013 IEEE 15th Electronics Packaging Technology Conference (EPTC 2013). IEEE, 2013. http://dx.doi.org/10.1109/eptc.2013.6745843.

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Bigoney, Burt, Scott Smith, and Michael Bruns. "A High-Performance Milling Machine for Aerospace Applications." In 2023 AeroTech. 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 2023. http://dx.doi.org/10.4271/2023-01-1002.

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<div class="section abstract"><div class="htmlview paragraph">As the aerospace industry moves toward determinate assembly and ever-tighter manufacturing tolerances, there is a need for automated, high-precision milling, trimming and drilling equipment that is specialized for aerospace applications. Precision countersinking is a common requirement for aircraft parts, but this is not a process that typical general-purpose milling machines are able to accommodate without the use of specialty tools such as depth-stop tool holders. To meet this need, Electroimpact has designed a 5-axis milling machine with high-speed clamping capability for countersink depth control. A custom trunnion and head with a quill and an additional clamp axis provide clamping functionality similar in speed and precision to a riveting machine, while maintaining the accuracy and features of a conventional machining center. An additional focus on design for pre-compensation accuracy has allowed the system to achieve post-compensation path and positioning tolerances that are competitive with premium milling machines. This combination of capabilities makes the system well suited for a variety of cutting and drilling processes for aircraft manufacture. This paper will describe the background and design process that led to the development of this system, and will provide details on its capabilities, specifications, and possible applications.</div></div>
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Stell, R. W. Benjamin. "A Review of Current Standards and Codes for Maximum Permissible Rail Voltage Rise on Direct Current Traction Power Systems." In 2011 Joint Rail Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/jrc2011-56121.

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The negative return portion of a modern direct current (dc) traction power system, which includes the tracks (the running rails), is normally isolated from earth to the maximum extent practical. The purpose of this isolation is to prevent stray dc currents from flowing through the earth and potentially causing corrosion of nearby metallic infrastructure. The isolation of the tracks from the earth is not perfect. Each track tie and insulated rail fastener assembly can be electrically represented as a resistor of high ohmic value connected between the rails and the earth. With many of these resistors in parallel over miles of track, a distributed “leakage resistance” is established between the rails and earth. For modern dc traction power systems in particular, however, this resistance is high enough for the rails to be considered essentially ungrounded with respect to local electrical ground (earth). The lack of an intentional connection between the tracks and earth allows voltage differences to occur along the rails, and between the rails and nearby structures. These voltage differences are caused by the flow of current through the running rails back to the substations. Since the shells of rail vehicles are typically at the same voltage as the wheels and rails, this voltage difference could be impressed on a passenger entering or exiting a train from a grounded platform. Or they could be impressed on a person walking along the tracks. In the USA, these voltage differences have generally been limited through system design; North American standards for substation grounding are referenced for design purposes, in particular IEEE Standard 80, Guide for Safety in Substation Grounding. In Europe, a standard has been developed specifically to address control of voltages between rails and structures, BS EN 50122-1 (IEC 62128-1), Railway Applications – Fixed Installations – Part 1: Protective Provisions Relating to Electrical Safety and Earthing. Voltage-limiting equipment that can be installed in passenger stations and other accessible locations has been developed in response to the requirements of EN 50122-1. These devices quickly connect the running rails to the station structure to eliminate unsafe voltage differences. If an earth fault occurs (broken catenary conductor falling on the ground, for example), there may not be a low-resistance circuit back to the substation due to the electrical isolation between running rails and earth ground. Without a low-resistance path back to the substation, there may be a resulting low-level short circuit current flow insufficient to operate the substation protective systems. As a result, the area in the vicinity of the fault may potentially be elevated to unsafe voltage levels. Equipment intended to detect this condition and connect the substation negative dc bus to the substation grounding grid is gradually being incorporated into modern North American dc traction power substation design. These devices are known by several names such as “substation grounding contactors”, “automatic grounding switches”, and “negative grounding devices”. Devices built to comply with EN 50122-1 are termed “Voltage Limiting Devices”. EN 50122-1 includes voltage-time curves that dictate the maximum permissible magnitudes and durations for ac and dc voltages; equipment built to EN 50122-1 must clamp the highest voltages in no more than 20 milliseconds. This paper will review current American and European standards and codes for maximum permissible rail voltage on direct current traction power systems. The maximum permissible voltage levels will be explained and compared. The principles of negative grounding device operation and corresponding voltage settings will also be discussed.
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