Relevant bibliographies by topics / AtPIP2

Academic literature on the topic 'AtPIP2'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "AtPIP2"

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Kim, Hyun-Mi, Hyun-Sik Hwang, Suk-Chan Lee, Su-Hyun Jo, and Beom-Gi Kim. "The Optimization for Functional Expression of Arabidopsis Thaliana AtPIP2-1 in Xenopus laevis Oocyte." Journal of Applied Biological Chemistry 53, no. 4 (December 31, 2010): 189–94. http://dx.doi.org/10.3839/jabc.2010.034.

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Li, Zhou, Jieru Hou, Yan Zhang, Weihang Zeng, Bizhen Cheng, Muhammad Jawad Hassan, Youzhi Zhang, Qi Pu, and Yan Peng. "Spermine Regulates Water Balance Associated with Ca2+-Dependent Aquaporin (TrTIP2-1, TrTIP2-2 and TrPIP2-7) Expression in Plants under Water Stress." Plant and Cell Physiology 61, no. 9 (June 16, 2020): 1576–89. http://dx.doi.org/10.1093/pcp/pcaa080.

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Abstract Spermine (Spm) regulates water balance involved in water channel proteins, aquaporins (AQPs), in plants. An increase in endogenous Spm content via exogenous Spm application significantly improved cell membrane stability, photosynthesis, osmotic adjustment (OA) and water use efficiency (WUE) contributing to enhanced tolerance to water stress in white clover. Spm upregulated TrTIP2-1, TrTIP2-2 and TrPIP2-7 expressions and also increased the abundance of TIP2 and PIP2-7 proteins in white clover under water stress. Spm quickly activated intracellular Ca2+ signaling and Spm-induced TrTIP2-2 and TrPIP2-7 expressions could be blocked by Ca2+ channel blockers and the inhibitor of Ca2+-dependent protein kinase in leaves of white clover. TrSAMS in relation to Spm biosynthesis was first cloned from white clover and the TrSAMS was located in the nucleus. Transgenic Arabidopsis overexpressing the TrSAMS had significantly higher endogenous Spm content and improved cell membrane stability, photosynthesis, OA, WUE and transcript levels of AtSIP1-1, AtSIP1-2, AtTIP2-1, AtTIP2-2, AtPIP1-2, AtPIP2-1 and AtNIP2-1 than wild type in response to water stress. Current findings indicate that Spm regulates water balance via an enhancement in OA, WUE and water transport related to Ca2+-dependent AQP expression in plants under water stress.
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Zhang, Renshan, Xiaoqian Guan, Meijing Yang, Yee-Song Law, Chia Pao Voon, Junran Yan, Feng Sun, and Boon Leong Lim. "Overlapping Functions of the Paralogous Proteins AtPAP2 and AtPAP9 in Arabidopsis thaliana." International Journal of Molecular Sciences 22, no. 14 (July 6, 2021): 7243. http://dx.doi.org/10.3390/ijms22147243.

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Arabidopsis thaliana purple acid phosphatase 2 (AtPAP2), which is anchored to the outer membranes of chloroplasts and mitochondria, affects carbon metabolism by modulating the import of some preproteins into chloroplasts and mitochondria. AtPAP9 bears a 72% amino acid sequence identity with AtPAP2, and both proteins carry a hydrophobic motif at their C-termini. Here, we show that AtPAP9 is a tail-anchored protein targeted to the outer membrane of chloroplasts. Yeast two-hybrid and bimolecular fluorescence complementation experiments demonstrated that both AtPAP9 and AtPAP2 bind to a small subunit of rubisco 1B (AtSSU1B) and a number of chloroplast proteins. Chloroplast import assays using [35S]-labeled AtSSU1B showed that like AtPAP2, AtPAP9 also plays a role in AtSSU1B import into chloroplasts. Based on these data, we propose that AtPAP9 and AtPAP2 perform overlapping roles in modulating the import of specific proteins into chloroplasts. Most plant genomes contain only one PAP-like sequence encoding a protein with a hydrophobic motif at the C-terminus. The presence of both AtPAP2 and AtPAP9 in the Arabidopsis genome may have arisen from genome duplication in Brassicaceae. Unlike AtPAP2 overexpression lines, the AtPAP9 overexpression lines did not exhibit early-bolting or high-seed-yield phenotypes. Their differential growth phenotypes could be due to the inability of AtPAP9 to be targeted to mitochondria, as the overexpression of AtPAP2 on mitochondria enhances the capacity of mitochondria to consume reducing equivalents.
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Ferrari, Simone, Roberta Galletti, Donatella Vairo, Felice Cervone, and Giulia De Lorenzo. "Antisense Expression of the Arabidopsis thaliana AtPGIP1 Gene Reduces Polygalacturonase-Inhibiting Protein Accumulation and Enhances Susceptibility to Botrytis cinerea." Molecular Plant-Microbe Interactions® 19, no. 8 (August 2006): 931–36. http://dx.doi.org/10.1094/mpmi-19-0931.

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Polygalacturonases (PGs) hydrolyze the homogalacturonan of plant cell-wall pectin and are important virulence factors of several phytopathogenic fungi. In response to abiotic and biotic stress, plants accumulate PG-inhibiting proteins (PGIPs) that reduce the activity of fungal PGs. In Arabidopsis thaliana, PGIPs with comparable activity against BcPG1, an important pathogenicity factor of the necrotrophic fungus Botrytis cinerea, are encoded by two genes, AtPGIP1 and AtPGIP2. Both genes are induced by fungal infection through different signaling pathways. We show here that transgenic Arabidopsis plants expressing an antisense AtPGIP1 gene have reduced AtPGIP1 inhibitory activity and are more susceptible to B. cinerea infection. These results indicate that PGIP contributes to basal resistance to this pathogen and strongly support the vision that this protein plays a role in Arabidopsis innate immunity.
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Ortiz-Morea, Fausto Andres, Daniel V. Savatin, Wim Dejonghe, Rahul Kumar, Yu Luo, Maciej Adamowski, Jos Van den Begin, et al. "Danger-associated peptide signaling in Arabidopsis requires clathrin." Proceedings of the National Academy of Sciences 113, no. 39 (September 20, 2016): 11028–33. http://dx.doi.org/10.1073/pnas.1605588113.

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The Arabidopsis thaliana endogenous elicitor peptides (AtPeps) are released into the apoplast after cellular damage caused by pathogens or wounding to induce innate immunity by direct binding to the membrane-localized leucine-rich repeat receptor kinases, PEP RECEPTOR1 (PEPR1) and PEPR2. Although the PEPR-mediated signaling components and responses have been studied extensively, the contributions of the subcellular localization and dynamics of the active PEPRs remain largely unknown. We used live-cell imaging of the fluorescently labeled and bioactive pep1 to visualize the intracellular behavior of the PEPRs in the Arabidopsis root meristem. We found that AtPep1 decorated the plasma membrane (PM) in a receptor-dependent manner and cointernalized with PEPRs. Trafficking of the AtPep1-PEPR1 complexes to the vacuole required neither the trans-Golgi network/early endosome (TGN/EE)-localized vacuolar H+-ATPase activity nor the function of the brefeldin A-sensitive ADP-ribosylation factor-guanine exchange factors (ARF-GEFs). In addition, AtPep1 and different TGN/EE markers colocalized only rarely, implying that the intracellular route of this receptor–ligand pair is largely independent of the TGN/EE. Inducible overexpression of the Arabidopsis clathrin coat disassembly factor, Auxilin2, which inhibits clathrin-mediated endocytosis (CME), impaired the AtPep1-PEPR1 internalization and compromised AtPep1-mediated responses. Our results show that clathrin function at the PM is required to induce plant defense responses, likely through CME of cell surface-located signaling components.
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Robinson, Whitney D., Joonho Park, Hue T. Tran, Hernan A. Del Vecchio, Sheng Ying, Jacqui L. Zins, Ketan Patel, Thomas D. McKnight, and William C. Plaxton. "The secreted purple acid phosphatase isozymes AtPAP12 and AtPAP26 play a pivotal role in extracellular phosphate-scavenging by Arabidopsis thaliana." Journal of Experimental Botany 63, no. 18 (November 2012): 6531–42. http://dx.doi.org/10.1093/jxb/ers309.

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TRAN, HUE T., WEIQIANG QIAN, BRENDEN A. HURLEY, YI-MIN SHE, DAOWEN WANG, and WILLIAM C. PLAXTON. "Biochemical and molecular characterization of AtPAP12 and AtPAP26: the predominant purple acid phosphatase isozymes secreted by phosphate-starved Arabidopsis thaliana." Plant, Cell & Environment 33, no. 11 (June 2, 2010): 1789–803. http://dx.doi.org/10.1111/j.1365-3040.2010.02184.x.

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Xu, Zhou, Renshan Zhang, Meijing Yang, Yee-Song Law, Feng Sun, Ngai Lung Hon, Sai Ming Ngai, and Boon Leong Lim. "A Balance between the Activities of Chloroplasts and Mitochondria Is Crucial for Optimal Plant Growth." Antioxidants 10, no. 6 (June 9, 2021): 935. http://dx.doi.org/10.3390/antiox10060935.

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Energy metabolism in plant cells requires a balance between the activities of chloroplasts and mitochondria, as they are the producers and consumers of carbohydrates and reducing equivalents, respectively. Recently, we showed that the overexpression of Arabidopsis thaliana purple acid phosphatase 2 (AtPAP2), a phosphatase dually anchored on the outer membranes of chloroplasts and mitochondria, can boost the plant growth and seed yield of Arabidopsis thaliana by coordinating the activities of both organelles. However, when AtPAP2 is solely overexpressed in chloroplasts, the growth-promoting effects are less optimal, indicating that active mitochondria are required for dissipating excess reducing equivalents from chloroplasts to maintain the optimal growth of plants. It is even more detrimental to plant productivity when AtPAP2 is solely overexpressed in mitochondria. Although these lines contain high level of adenosine triphosphate (ATP), they exhibit low leaf sucrose, low seed yield, and early senescence. These transgenic lines can be useful tools for studying how hyperactive chloroplasts or mitochondria affect the physiology of their counterparts and how they modify cellular metabolism and plant physiology.
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Shah, Dhvanit I., Naoko Takahasi-Makise, Iman Schultz, Eric L. Pierce, Liangtao Li, Guillaume Vogin, Benjamin S. Brigham, et al. "atpif1 regulates Mitochondrial Heme Synthesis In Developing Erythroid Cells." Blood 116, no. 21 (November 19, 2010): 163. http://dx.doi.org/10.1182/blood.v116.21.163.163.

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Abstract Abstract 163 Iron plays a key role as a cofactor in many fundamental metabolic processes, which require heme synthesis and Fe/S cluster assembly in the mitochondria. Defects in the transport of iron into the mitochondria would lead to anemias due to a deficiency in heme and hemoglobin synthesis. Here we describe a zebrafish genetic mutant, pinotage (pnttq209), which exhibits a profound hypochromic, microcytic anemia. Erythrocytes from pnt mutants have a defect in hemoglobinization and decreased red cell indices (mean corpuscular volume and hemoglobin content, hematocrit, hemoglobin concentration). Through positional cloning, we showed that the mitochondrial ATPase Inhibitory Factor 1 (atpif1), which regulates the inner mitochondrial membrane potential, is the gene disrupted in pnt. The identity of the pnt gene was verified by: (a) decreased atpif1 steady-state mRNA in pnt mutants, (b) phenocopying the anemia with anti-sense atpif1 morpholinos, (c) functional complementation of the anemia with atpif1 cRNA, and (d) a genetic polymorphism in the 3'UTR co-segregating with the mutant phenotype that destabilizes the atpif1 mRNA. Consistent with the conserved function of atpif1 in higher vertebrates, the silencing of the murine ortholog of atpif1 in Friend mouse erythroleukemia (MEL) cells showed a defect in hemoglobinization by o-dianisidine staining and reduction of 59Fe incorporation into heme in 59Fe-metabolically labeled cells. Moreover, Atpif1 knockdown destabilizes their mitochondrial membrane potential and volume. Therefore, the identification of atpif1 in pnt functionally demonstrates the role of atpif1 in regulating the proton motive gradient across the inner mitochondrial membrane for mitochondrial iron incorporation in heme biosynthesis. These results uncover a novel hematopoiesis-related function of atpif1, which will directly contribute to our understanding and potential treatment of human congenital and acquired anemias. Disclosures: No relevant conflicts of interest to declare.
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Shah, Dhvanit I., Naoko Takahashi-Makise, Jeffrey D. Cooney, Liangtao Li, Iman Schultz, Eric L. Pierce, Anupama Narla, et al. "Mitochondrial Atpif1 Regulates Heme Synthesis in Developing Erythroblasts." Blood 118, no. 21 (November 18, 2011): 343. http://dx.doi.org/10.1182/blood.v118.21.343.343.

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Abstract Abstract 343 Iron and protoporphyrin IX (PPIX) are key substrates used by ferrochelatase (Fech) to produce heme, which subsequently binds to globin to generate hemoglobin in red blood cells. Defects in the transport of iron, synthesis of PPIX, or catalytic activity of Fech impair heme synthesis, and thus cause human congenital anemias. However, the precise mechanisms regulating transporters and enzymes facilitating heme synthesis and their inter-dependent actions remain largely unknown. The zebrafish mutant pinotage (pnttq209) exhibits profound hypochromic, microcytic anemia. Erythrocytes from viable adult pnt fish have reduced hemoglobin content and cell volume. Positional cloning, morpholino-induced loss-of-function, cRNA over-expression, quantitative RT-PCR, and mutational analysis show that mitochondrial ATPase inhibitory factor1 (Atpif1) is the gene disrupted in pnt. Previous studies have demonstrated the role of Atpif1 in the regulation of mitochondrial proton motive force, pH, and ATP synthesis. Here, we report direct evidence that Atpif1 regulates mitochondrial heme synthesis. Knock down of the human and murine orthologs of Atpif1 using shRNAs in mammalian erythroid tissues, human CD34+, mouse Friend erythroleukemia (MEL) and primary fetal liver cells, impairs hemoglobinization. Atpif1 protein levels are reduced in stable MEL cells silenced for Atpif1; however, the protein levels of other mitochondrial structural proteins, β-subunit of ATP synthase (AtpB), voltage-dependent anionic-selective channel protein 1 (Vdac1), complex IV (CoxIV), and heat shock protein 60 (Hsp60), are normal, indicating an intact mitochondrial structure in Atpif1 silenced cells. Moreover, Atpif1 silenced cells have an increased mitochondrial membrane potential, an elevation in mitochondrial pH, and depleted ATP levels. Differentiating MEL cells silenced for Atpif1 display reduced incorporation of 59Fe into heme, although their mitochondria have sufficient amounts of Fech substrates, iron and PPIX. This is due to diminished in vivo Fech activity, despite having normal levels of Fech protein in Atpif1 silenced cells. We further establish that the Fech activity is reduced at pH 8.5, corresponding to the more alkaline mitochondrial pH in Atpif1-silenced cells. The over-expression of either yeast Fech or zebrafish Fech in pnt embryos shows that only yeast Fech rescues the anemia in pnt. This demonstrates that the catalytic activity of [Fe-S] bound zebrafish Fech, and not yeast Fech, which lacks the [Fe-S] cluster, is vulnerable to the elevation in mitochondrial pH due to the loss of Atpif1. Therefore, the loss of Atpif1 reduces the catalytic ability of vertebrate Fech to incorporate iron into PPIX to make heme, resulting in hypochromic anemia. The newly uncovered role of Atpif1 to regulate Fech provides a new insight on the mitochondrial regulation of heme synthesis and a potential cause of sideroblastic anemias. Mechanistic model of Atpif1 function in heme synthesis. The mitochondrial Atpif1 normally preserves mitochondrial pH. Loss of Atpif1 alkalinizes mitochondrial pH, the [Fe-S] cluster binding makes Fech sensitive to mitochondrial pH changes, and consequently reduces its catalytic efficiency for the production of heme. Disclosures: No relevant conflicts of interest to declare.
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Dissertations / Theses on the topic "AtPIP2"

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Zhang, Renshan, and 张仁善. "Functional analysis of two arabidopsis purple acid phosphatases : AtPAP2 and AtPAP9." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2015. http://hdl.handle.net/10722/211114.

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Law, Yee-song, and 劉益松. "Biological roles of atpap2 in the mitochondria." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2015. http://hdl.handle.net/10722/211155.

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Zhang, Youjun, and 张有君. "Growth promoting effects of AtPAP2 in potato and camelina." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46476945.

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Silverstein, Robert S. "Regulation of the Atp2b2 gene /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/10656.

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Douville, Élise. "Structure, assemblage et comportement hydrodynamique d'AtPP2-A1 et AtPP2-A2 chez Arabidopsis thaliana." Nantes, 2010. http://www.theses.fr/2010NANT2093.

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Chez les Dicotylédones, les tubes criblés contiennent du saccharose transporté à longue distance par flux de masse et des inclusions protéiques, appelées protéines P. Chez A. Thaliana, les protéines P se trouvent sous forme de filaments et des études d’immunolocalisation ont confirmé que les PP2s sont associées aux protéines P. Deux gènes PP2 sont exprimés spécifiquement dans le phloème: AtPP2-A1 et AtPP2-A2. A partir d’observations microscopiques, il a été émis l’hypothèse que les protéines P pourraient, en réponse à des blessures du phloème, participer à l'occlusion des tubes criblés par des mécanismes d’assemblage particuliers. Afin de vérifier cette hypothèse, les protéines recombinantes PP2-A1 et PP2-A2 ont été produites dans E. Coli et leurs structure et autoassemblage ont été étudiés dans différentes conditions physico-chimiques, proche des conditions de la sève élaborée. Les protéines PP2-A1 et PP2-A2 sont présentes en solution sous forme d'oligomères allongés quel que soit le pH, avec un degré d'oligomérisation N plus élevé pour PP2-A2 que pour PP2-A1. Ces résultats obtenus par diffusion des rayons X aux petits angles et les observations réalisées en microscopie électronique, suggèrent que les oligomères s'auto-associent pour former des structures torsadées filamenteuses. L’augmentation de la force ionique amplifie l’auto-association de PP2-A1 et A2. En revanche, la présence de calcium, de saccharose ou la vitesse de cisaillement n’affecte pas l’auto-association de PP2-A1. L’ensemble de ces résultats suggèrent que la présence de PP2 n’altère pas le flux de sève élaborée dans les tubes criblés
Dicotyledonous sieve elements typically contain saccharose transported at long distances by mass flow and protein inclusions, called P-Proteins. In Arabidopsis thaliana, P-proteins are found as filaments and immunolocalization studies had confirmed that PP2s are indeed associated to Pproteins. Two PP2 genes were shown to be expressed specifically in the phloem: AtPP2-A1 and AtPP2-A2. Based on observations in microscopy, it was hypothesized that P proteins could, in response to injury of the phloem, participate in the occlusion of sieve tubes by peculiar assembly mechanisms. In order to check this hypothesis, PP2-A1 and PP2-A2 recombinant proteins were produced in E. Coli and their structure and self-assembly properties were studied under different physical chemical conditions, close to phloem sap conditions. Proteins PP2-A1 and PP2-A2 were present in solution as elongated shape oligomers whatever the pH, with a degree of oligomerization N being higher for PP2-A2 than for PP2-A1. These results obtained by small angle X-ray scattering together with observations using transmission electron microscopy suggest that the oligomers self-associate to form an arrangement of filamentous and twisted structures. Increasing ionic strength enhances the proteins self-association. By the contrary, the presence of calcium, sucrose or shear rate do not affect PP2-A1 self-association. Alltogether, these results suggest that the presence of PP2 proteins within phloem sieve tubes do not compromise mass flow
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Tietz, Olaf. "Funktionelle Charakterisierung des AtPIN1-Proteins aus Arabidopsis thaliana." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=969066104.

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Hütter, Michael. "Rolle von CACNA1A, ATP1A2 und SCN1A für die sporadische hemiplegische Migräne." Diss., lmu, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-101418.

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Nuntasoontorn, Komsun. "Functional analysis of the Arabidopsis thaliana meiotic proteins AtPCH2 and AtCHR24." Thesis, University of Birmingham, 2014. http://etheses.bham.ac.uk//id/eprint/4921/.

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In the past decade Arabidopsis thaliana has become an important system for studying meiosis in flowering plants. The identification of meiotic mutants has provided an important approach to studying plant meiosis. The availability of the Arabidopsis genome sequence together with developments in proteomics and bioinformatics provides an additional route for the identification of meiotic proteins and analysis of their functional interrelationships. This study has used a proteomics approach to identify a member of the SWI2/SNF2 chromatin remodelling gene family (Atchr24). Although a variety defects was observed in Atchr24 male meiocytes cytogenetic, at least two T-DNA insertion lines on this gene appear normal. Secondly, this research has also used a bioinformatics approach to identify a potential orthologue of Pch2/TRIP13 in Arabidopsis. PCH2 (Pachytene checkpoint 2) is a member of the AAA+ ATPase family of proteins. This study reveals that AtPCH2 plays an essential role in the controlled formation of meiotic crossovers (COs). Cytogenetic analysis of two Atpch2 T-DNA insertion lines revealed a high frequency of univalents at MI. The number of chiasmata (COs) is reduced to ~ 70% of wild-type (WT). Genetic analysis revealed that Atpch2 has significantly weaker CO interference than WT leading to a redistribution of COs along the chromosomes. The recombination defect is accompanied by incomplete chromosome synapsis. Immunolocalisation of the chromosome axis protein AtASY3 and cohesin, AtSYN1 appears normal. However in contrast to WT, AtASY1 co-localises with the synaptonemal protein AtZYP1 in ii Atpch2 rather than becoming depleted in regions of synapsis and the meiotic progression of Atpch2 is delayed during pachytene by ~5 hours. These observations suggest a defect in remodeling of the chromosome axes and highlight how this process is essential for normal CO control.
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Stavridou, Ioanna Stavros. "Novel binding partners of an Arabidopsis phosphatidylinositol phosphate 5-kinase, AtPIPKβ." Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.615136.

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Attílio, Lísia Borges. "Transformação genética de laranja doce (Citrus sinensis L. Osbeck) com o gene D4E1 dirigido pelos promotores CaMV35S ou AtPP2." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-23042013-162934/.

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O Brasil é o maior produtor de laranja doce do mundo. O histórico da citricultura brasileira é marcado por uma sucessão de doenças causadas por diferentes agentes etiológicos. Entre as principais doenças que afetam a cultura, têm levado a maiores prejuízos, as bacterianas, com destaque para o cancro cítrico causado por Xanthomonas citri subsp. citri e o Huanglongbing associado a três espécies de \"Candidatus Liberibacter\". Devido à ausência de cultivares de laranja doce resistentes a estas doenças, a transformação genética é uma alternativa promissora para obtenção de plantas resistentes. Uma das estratégias no uso da transgenia para conferir ação contra bactérias é a inserção de genes que codificam peptídeos antimicrobianos como o D4E1, um peptídeo sintético, que tem apresentado eficiência no controle de doenças fúngicas e bacterianas de várias culturas, in vivo e in vitro. Este trabalho foi realizado com o objetivo de obter plantas transgênicas de laranja doce das cultivares \'Hamlin\', \'Pêra\' e \'Valência\', via Agrobacterium tumefaciens, expressando o gene D4E1, dirigido pelos promotores CaMV35S (Cauliflower mosaic virus 35S promoter) de expressão constitutiva ou pelo AtPP2 (Arabidopsis thaliana phloem protein 2) com expressão preferencial no floema, visando obter plantas resistentes a doenças bacterianas. Foram obtidas 13 plantas transgênicas da cultivar \'Hamlin\', 10 da cultivar \'Pêra\' e 8 da cultivar \'Valência\', contendo a construção gênica CaMV35S/D4E1 e 19 plantas transgênicas da cultivar \'Hamlin\', 6 da cultivar \'Pêra\' e 15 da cultivar \'Valência\' contendo a construção gênica AtPP2/D4E1. As plantas transgênicas apresentaram um a três eventos de inserção do T-DNA no genoma. Os níveis de expressão do transgene dirigido pelo promotor de expressão preferencial no floema foi menor comparado ao das plantas contendo o transgene dirigido pelo promotor de expressão constitutiva. Os resultados da expressão do transgene permitem selecionar plantas com maior expressão de cada uma das construções gênicas, para que, futuramente, estas sejam multiplicadas e avaliadas quanto à resistência ao cancro cítrico e ao HLB.
Brazil is the largest sweet orange producer in the world. The history of the Brazilian citrus industry is marked by a series of diseases caused by different etiologic agents. Among the diseases affecting the culture, those caused by bacteria are the ones that have caused more significant losses, especially the citrus canker caused by Xanthomonas citri subsp. citri, and huanglongbing (HLB) associated with three \"Candidatus Liberibacter\" bacteria species. Due to the absence of genetic resistance to these diseases in commercial sweet orange cultivars, the genetic transformation is a promising alternative to produce resistant plants. One of the strategies to produce transgenic resistant plants to bacteria is the use of genes that code for antimicrobial peptides, such as D4E1, a antimicrobial synthetic peptide, which has shown efficient results controlling diseases caused by bacteria and fungi in several crops, through in vitro and in vivo experiments. The aim of this study was to produce \'Hamlin\', \'Pêra\' and \'Valencia\' sweet orange transgenic plants, via Agrobacterium tumefaciens, expressing the D4E1 gene driven by the constitutive promoter Cauliflower mosaic virus (CaMV35S) or Arabidopsis thaliana phloem protein 2 (AtPP2), a promoter preferentially expressed in the phloem. It was possible to regenerate 13 \'Hamlin\' transgenic lines, 10 \'Pêra\' transgenic lines and 8 \'Valencia\' transgenic lines bearing the gene construct CaMV35S/D4E1, whereas 19 \'Hamlin\' transgenic lines, 6 \'Pêra\' transgenic lines and 15 \'Valencia\' transgenic lines bearing the AtPP2/D4E1 gene construct were regenerated. The transgenic plants had one to three T-DNA insertion events in the genome. The transgene expression levels in transgenic plants for D4E1 gene driven by the phloem preferential promoter were lower than the transgenic expression levels of the transgene driven by the constitutive promoter. Transgene expression levels results may allow the selection of those plants with higher expression levels of each genetic construct for future multiplication and evaluation for citrus canker and HLB resistance.
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Book chapters on the topic "AtPIP2"

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Kohzuma, Kaori, Cristina Dal Bosco, Atsuko Kanazawa, David M. Kramer, and Jörg Meurer. "A Potential Function for the γ2 Subunit (atpC2) of the Chloroplast ATP Synthase." In Advanced Topics in Science and Technology in China, 576–78. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-32034-7_123.

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Kohzuma, Kaori, Cristina Dal Bosco, Atsuko Kanazawa, David M. Kramer, and Jörg Meurer. "A Potential Function for the γ2 Subunit (atpC2) of the Chloroplast ATP Synthase." In Advanced Topics in Science and Technology in China, 193–96. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-32034-7_40.

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Scaramuzzi, Carol D., Roger G. Hiller, Pamela M. Wrench, Peter J. Lockhart, and Anthony W. D. Larkum. "Sequence of atpI;atpH Genes of Prochloron sp. ATPase Gene Cluster: Implications for Chloroplast Origin." In Photosynthesis: from Light to Biosphere, 951–54. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-009-0173-5_226.

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Bortolozzi, Mario. "Defects in the Atp2b2 Gene Causing Hereditary Hearing and Balance Loss in Mice and Humans: A Biophysical Study of Normal and Mutated PMCA2 Pump Function." In Biophotonics: Spectroscopy, Imaging, Sensing, and Manipulation, 371. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9977-8_23.

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Gonschior, P., M. Fleuchaus, F. Gerheuser, B. Mack, A. E. Goetz, and B. Höfling. "Vergleich der in-vitro Aufnahme und Verteilung von liposomal gekoppelten Porphycenen (HEPn), acetylierten Porphycenen (ATPPn) und Hematoporphyrinderivaten (HPD) in kultiviertem humanem Plaquegewebe." In Laser in der Medizin / Laser in Medicine, 141–43. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-93548-0_32.

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HUFFAKER, ALISA, YUBE YAMAGUCHI, GREGORY PEARCE, and CLARENCE A. RYAN. "AtPep1 Peptides." In Handbook of Biologically Active Peptides, 5–8. Elsevier, 2006. http://dx.doi.org/10.1016/b978-012369442-3/50005-2.

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Wexler, Deborah J., and David M. Nathan. "Diabetes Mellitus: Control and Complications." In The Brigham Intensive Review of Internal Medicine, 517–26. Oxford University Press, 2014. http://dx.doi.org/10.1093/med/9780199358274.003.0052.

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Metabolic syndrome (MetS), also referred to as the insulin resistance syndrome or syndrome X, refers to a constellation of metabolic abnormalities that tend to cluster together and lead to a substantial increase in risk of atherosclerotic cardiovascular disease (CVD). Although manifestations of MetS have been recognized since the 1920s, it was first described as a syndrome by Gerald Reaven in 1988. The most commonly used definition of MetS in the United States is the one proposed by the National Cholesterol Education Program's Adult Treatment Panel III (NCEP ATPIII). The definition was first published in 2001 and then updated in 2004 (see table 52.1); however, there are other definitions as well (see table 52.2). Most definitions include insulin resistance (IR) or abdominal obesity as the essential criterion. The NCEP definition does not require the presence of IR or obesity as an essential criterion. However, most individuals diagnosed with MetS according to the NCEP definition are both obese and insulin resistant.
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Ho, Yugong, Stephen A. Liebhaber, and Nancy E. Cooke. "Distinct Patterns of Histone Modifications Are Mediated by the Early and Late Developmental Enhancers atPit-1Chromatin Locus." In The Endocrine Society's 92nd Annual Meeting, June 19–22, 2010 - San Diego, OR19–3—OR19–3. Endocrine Society, 2010. http://dx.doi.org/10.1210/endo-meetings.2010.part3.or1.or19-3.

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Conference papers on the topic "AtPIP2"

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Qian, Yanrong, Xuan Wang, Yunsheng Li, Yanyang Cao, and Xiaozhuo Chen. "Abstract 33: NSCLC cells internalize ATPin vitroandin vivousing multiple endocytotic pathways." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-33.

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Yang, Kang, Xi Zhao, Jianhua Zou, and Wan Du. "ATPP: A Mobile App Prediction System Based on Deep Marked Temporal Point Processes." In 2021 17th International Conference on Distributed Computing in Sensor Systems (DCOSS). IEEE, 2021. http://dx.doi.org/10.1109/dcoss52077.2021.00028.

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Zhu, Yanmin, Xiang Zheng, Xinrong Wu, Wanning Liu, Lei Pi, and Meiju Chen. "DPTCN-ATPP: Multi-scale End-to-end Modeling for Single-channel Speech Separation." In 2021 5th International Conference on Communication and Information Systems (ICCIS). IEEE, 2021. http://dx.doi.org/10.1109/iccis53528.2021.9645957.

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Novak, Damir, Michael Loetzerich, and Matthias Boese. "Aerodynamic Design and Testing of an Axial Flow Compressor for the GT24/26 Gas Turbines." In ASME Turbo Expo 2013: Turbine Technical Conference and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/gt2013-95067.

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A 22-stage axial flow compressor with a pressure ratio 35:1 has been designed, built and successfully tested for a heavy-duty gas turbine application. Advanced technology and aero engine design tools have been used. The compressor has been designed using an “arbitrary” airfoil blading including 3D design features, like leading edge re-camber, lean, sweep and flowpath contouring. The compressor performance and part load behavior have been improved by accurate stage matching based on whole compressor 3D analyses. The new compressor has been tested in a scaled down rig and validated in the Alstom Test Power Plant (ATPP).The compressor met all design objectives and demonstrated excellent performance. This paper describes the aerodynamic design and test results.
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