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Dissertations / Theses on the topic 'ATP aptamer'

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Published: 10 December 2022

Last updated: 28 January 2023

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1

Santos, Cancel Mirelis. "Development of Electrochemical Sensors with Enhanced Specificity and Temporal Resolution for Biological Applications." University of Cincinnati / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1553613866098747.

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2

Reinemann, Christine. "Aptamere als neue molekulare Erkennungselemente in Biosensoren /." Leipzig : Helmholtz-Zentrum für Umweltforschung, 2007. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=016271117&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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3

Haarberg, Hans Eirik. "Theophylline IMAGEtags (intracellular multi aptamer genetic tags) the development and evaluation of an RNA reporter system based on the theophylline aptamer /." [Ames, Iowa : Iowa State University], 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1464205.

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4

Dalton, Colette. "Aptamers as biosensors." Thesis, University of Strathclyde, 2010. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=15484.

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5

Park, Euisun. "Probing dynamics of ERBB3 receptor signaling with an RNA aptamer." Diss., Restricted to subscribing institutions, 2008. http://proquest.umi.com/pqdweb?did=1790313701&sid=2&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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6

Held, Daniel M. "RNA aptamer inhibitors of HIV reverse transcriptase molecular evolution and determinants of target specificity /." [Bloomington, Ind.] : Indiana University, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3219914.

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Thesis (Ph. D.)--Indiana University, Dept. of Biology, 2006.
"Title from dissertation home page (viewed June 28, 2007)." Source: Dissertation Abstracts International, Volume: 67-06, Section: B, page: 2963. Adviser: Donald H. Burke.

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7

Kissel, Jay D. "Target specificity and structural characterization of single-stranded DNA aptamer RT1t49, a broad inhibitor of HIV-1 reverse transcriptases." [Bloomington, Ind.] : Indiana University, 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3274917.

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Thesis (Ph.D.)--Indiana University, Dept. of Biology, 2007.
Source: Dissertation Abstracts International, Volume: 68-07, Section: B, page: 4320. Adviser: Donald H. Burke-Aguero. Title from dissertation home page (viewed Apr. 22, 2008).

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8

Pappas, Allison L. "Selection and characterization of RNA aptamers that detect a quaternary structure for ribosomal protein S7." [Ames, Iowa : Iowa State University], 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3369933.

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9

Cash, Daniel Dennis. "Solution NMR studies of the Class II GTP-binding RNA aptamer complex to determine the role of conserved residues in complex formation." Connect to online resource, 2008. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1456668.

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10

Rato, Carla Cristina Pereira Salgueiro Catarino. "The life marker chip : potential use of aptamers against small molecules and consideration of instrument planetary protection." Thesis, Cranfield University, 2013. http://dspace.lib.cranfield.ac.uk/handle/1826/8272.

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The Life Marker Chip (LMC) instrument was developed with the aim to detect evidence of life on Mars. The detection was based on an inhibition immunoassay. In this work aptamers were evaluated as potential alternative to antibodies for the LMC. Aptamers were synthetic oligonucleotides able to bind specifically with high affinity to a wide range of target molecules, and have been also integrated as bioreceptors in several detection instruments. The generation of new aptamers against two small molecules using the FluMag-SELEX method was tested and was verified the adaptability of pre-existing aptamers against small targets to the LMC assay type. Based on the fact that the LMC was going to be integrated into the space programme ExoMars, it was also implemented into a small scale experiment the Planetary Protection and Contamination Control requirements found on a life-search mission. In addition to that aptamers compatibility with a sterilisation procedure used in life-search missions was also tested. Furthermore because of the nature of the small molecules studied, multiple analytical chemistry techniques were assessed to verify covalent chemistry surface immobilisation. Within the project timeline it was not possible to achieve a full aptamer generation process but it was possible to understand the methodology behind the procedure and give input for future work. It was found that the direct implementation of existing aptamers against small molecules into the LMC assay was not successful. It was also seen that in the case of aptamer integration onto the LMC some assay changes would probably have to be made. This information was very useful to understand if aptamers could be an alternative to antibodies and be implemented directly into the LMC. It was found that aptamers survived the preliminary sterilisation method applied, which might open the possibility of making aptamers convenient space bioreceptors, reducing time and costs of instrument Planetary Protection implementation. In conclusion aptamers were not straightforward alternatives to antibodies for the LMC because aptamers interacted differently with their targets in comparison to antibodies, particularly with small molecules. Also the biochemical simplicity of the small molecule targets introduced difficulties in aptamers generation that more complex targets would have not. Although aptamers shown incompatibility with the LMC assay format against small targets, they presented resilience to a sterilisation procedure implemented on space missions which could lead to the development of more robust bioreceptors for space missions. This information was helpful in understanding which assay formats were better for detection of small molecules using aptamers and that might contribute for future assay choices applied in detection instruments. It was also possible to make recommendations for the LMC regarding design and validation methods used in life-search missions based on the lessons learn from the developed of a small scale experiment. The developed work was presented at conferences and mentioned in an article journal, and in that way contributed to the knowledge of the space community in general.

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11

Tageson, Mackenzie Elizabeth. "FUNCTIONAL 3-D CELLULOSE & NITROCELLULOSE PAPER-BASED, MULITPLEX DIAGNOSTIC PLATFORMS WITHOUT COUPLING AGENTS." DigitalCommons@CalPoly, 2013. https://digitalcommons.calpoly.edu/theses/1128.

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The purpose of this thesis was to demonstrate device functionality of 3-D paper-based, multiplex platforms, µPADs, without the use of coupling agents between layers. Previously, these platforms were fabricated with double-sided tape and cellulose powder to try to augment proper fluid routing, but difficulties with this method occurred. An acrylic housing unit with strategically placed pressure tabs was designed to aid horizontal and vertical fluid routing through the platform, thus eliminating the inconsistencies associated with coupling agents. Channel characterization studies, a COMSOLTM simulation, and development time studies were performed to aid device design and demonstrate device functionality. The implementation of this µPAD platform as a diagnostic instrument was validated via lateral flow immunoassays utilizing both biotinylated antibodies and biotinylated aptamers as capture reagents. Successful detection of the target analyte, IgE, as well as successful fluid routing through multiple layers of membrane was demonstrated by immunoassays performed on 3-D, multiplex platforms. Another important result determined the aptamers’ ability to detect IgE to be statistically the same as the antibodies’ ability; thus confirming aptamers as viable capture reagent alternatives to antibodies in lateral flow assays. Overall, this research project was performed to develop and validate via experiment a prototype paper-based microfluidic diagnostic device, µPAD, with the capability to detect multiple biomarkers on one platform.

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12

Vantourout, Pierre. "Rôle de l'Ecto-F1-APTase dans la reconnaissance des cellules tumorales par les lymphocytes T Vgamma9/Vdelta2." Toulouse 3, 2009. http://thesesups.ups-tlse.fr/608/.

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Les lymphocytes T Vgamma9/Vdelta2 ont originellement été décrits pour être activés par de petites molécules (phosphoantigènes) isolées à partir de Mycobacterium tuberculosis, agent pathogène responsable de la tuberculose chez l'homme. Ces lymphocytes ont également un large potentiel antitumoral qui pourrait être utilisé pour le développement d'immunothérapies anticancéreuses. Nous nous intéressons aux mécanismes d'activation des lymphocytes T Vgamma9/Vdelta2 par les cellules tumorales et avons récemment identifié l'Ecto-F1-ATPase comme antigène impliqué dans leur reconnaissance. Les travaux effectués au cours de cette thèse mettent en évidence une interaction entre cette molécule et le Complexe Majeur d'Histocompatibilité de classe I, connu pour réguler l'activation de ces lymphocytes. Nous avons également obtenu des données indiquant que l'Ecto-F1-ATPase peut présenter des phosphoantigènes aux lymphocytes T Vgamma9/Vdelta2
Vgamma9/Vdelta2 T cells have originally been described to be activated by small molecules (phosphoantigens) purified from Mycobacterium tuberculosis, the pathogen responsible for the development of tuberculosis in humans. These lymphocytes also have a broad antitumoral potential which could be exploited for the development of anticancer immunotherapy. We are studying the mechanisms by which tumor cells activate Vgamma9/Vdelta2 T cells and have recently identified the Ecto-F1-ATPase as an antigen involved in their recognition. Our results, gathered in this thesis, show an interaction between this complex and Major Histocompatibility Class I molecules, which are known to regulate the activation of these lymphocytes. We also provide experimental data showing that Ecto-F1-ATPase can present phosphoantigens to Vgamma9/Vdelta2 T cells.

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13

Antoni, Florent. "Conception & études de biodistribution de liposomes ciblant CD44 dans un modèle de cancer du sein." Thesis, Sorbonne Paris Cité, 2019. http://www.theses.fr/2019USPCC063.

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Ce projet a eu pour objectif d’étudier la biodistribution in vivo de nanoparticules liposomales ciblant CD44, et en particulier d’évaluer l’effet de l’agent de ciblage (aptamère anti-CD44 greffé en surface des liposomes) sur leur accumulation tumorale. Des liposomes anti-CD44 fluorescents et radiomarqués pour l’imagerie en Tomographie par Emission de Positons ont été conçus. La méthode que nous avons développée a l’avantage d’être applicable à tout type de nanoparticule liposomale, quel que soit l’agent de ciblage. Les résultats de biodistribution obtenus chez des souris porteuses de xénogreffes de cancer du sein surexprimant CD44 ont montré que les liposomes ciblant CD44 ont une accumulation tumorale supérieure à celle des liposomes sans antigène cible, et que cette différence est liée à l’interaction spécifique Aptamère-CD44. Ceci confirme l’intérêt des liposomes fonctionnalisés avec l’aptamère anti-CD44 à visée thérapeutique (après chargement en drogues) dans les types tumoraux surexprimant CD44. Enfin les nanoparticules que nous avons développées constituent de bons agents d’ « imagerie compagnon », permettant de vérifier en imagerie isotopique l’accumulation tumorale de leurs équivalents thérapeutiques avant administration chez les patients
This project aimed to study the in vivo biodistribution of liposomal nanoparticles targeting CD44, and particularly to evaluate the effect of the targeting agent (anti-CD44 aptamer grafted on the surface of liposomes) on their tumor accumulation. Fluorescent and radiolabeled anti-CD44 liposomes for Positron Emission Tomography imaging were designed. The method we developed has the advantage of being applicable to any type of liposomal nanoparticle, regardless of the targeting agent. The biodistribution results obtained in mice bearing CD44 overexpressing breast cancer xenografts showed that CD44-targeting liposomes had greater tumor accumulation than liposomes without a target antigen, and that this difference was related to the specific Aptamer-CD44 interaction. This confirms the interest of liposomes functionalized with the anti-CD44 aptamer as therapeutic agents (after loading in drugs) in tumor types overexpressing CD44. Finally, the nanoparticles that we have developed are good "companion imaging" agents, making it possible to verify by isotopic imaging the tumor accumulation of the therapeutic counterparts before their administration in patients

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14

"In vitro selection of aptamers and protein." Doctoral diss., 2013. http://hdl.handle.net/2286/R.I.18047.

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abstract: Since Darwin popularized the evolution theory in 1895, it has been completed and studied through the years. Starting in 1990s, evolution at molecular level has been used to discover functional molecules while studying the origin of functional molecules in nature by mimicing the natural selection process in laboratory. Along this line, my Ph.D. dissertation focuses on the in vitro selection of two important biomolecules, deoxynucleotide acid (DNA) and protein with binding properties. Chapter two focuses on in vitro selection of DNA. Aptamers are single-stranded nucleic acids that generated from a random pool and fold into stable three-dimensional structures with ligand binding sites that are complementary in shape and charge to a desired target. While aptamers have been selected to bind a wide range of targets, it is generally thought that these molecules are incapable of discriminating strongly alkaline proteins due to the attractive forces that govern oppositely charged polymers. By employing negative selection step to eliminate aptamers that bind with off-target through charge unselectively, an aptamer that binds with histone H4 protein with high specificity (>100 fold)was generated. Chapter four focuses on another functional molecule: protein. It is long believed that complex molecules with different function originated from simple progenitor proteins, but very little is known about this process. By employing a previously selected protein that binds and catalyzes ATP, which is the first and only protein that was evolved completely from random pool and has a unique α/β-fold protein scaffold, I fused random library to the C-terminus of this protein and evolved a multi-domain protein with decent properties. Also, in chapter 3, a unique bivalent molecule was generated by conjugating peptides that bind different sites on the protein with nucleic acids. By using the ligand interactions by nucleotide conjugates technique, off-the shelf peptide was transferred into high affinity protein capture reagents that mimic the recognition properties of natural antibodies. The designer synthetic antibody amplifies the binding affinity of the individual peptides by ∼1000-fold to bind Grb2 with a Kd of 2 nM, and functions with high selectivity in conventional pull-down assays from HeLa cell lysates.
Dissertation/Thesis
Ph.D. Biochemistry 2013

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15

Yun, Seonho. "Intracellular Microenvironment Triggered Co-delivery of Anticancer Drugs and Genes." Thesis, 2019. http://hdl.handle.net/2440/120605.

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Currently, infallible cancer treatment has not been achieved. Doxorubicin (DOX), an active anticancer drug, initiates the apoptotic signaling pathway by intercalating topoisomerase-II, resulting in DNA replication disorder and producing reactive oxygen species that can damage DNA and activate cell death receptors. However, long-term DOX administration is always accompanied with serious multidrug resistance (MDR) and adverse side-effects. Therefore, delivery systems for DOX and gene drug, such as miRNA-21 inhibitor (miR-21i) which mitigates MDR, have been developed to reduce side-effects caused by non-selective cellular uptake of co-drugs in the absence of suitable delivery systems. However, difficulties in designing delivery systems for different types of drugs to achieve both high therapeutic efficacy and low side-effects have been reported. In this thesis, various biodegradable and biocompatible smart microgels are developed for DOX and gene co-delivery to achieve maximal anticancer performance and minimal side-effects. The microgel delivery carriers are prepared by crosslinking biocompatible low molecular weight polyethyleneimine (PEI800) using glutathione-cleavable diselenide crosslinkers. To the carriers, DOX is conjugated via various intracellular microenvironment sensitive linkers, and genes are electrostatically loaded to glutathione-cleavable polyethyleneimine. Finally, anionic hyaluronic acid (HA) surface coating is able to inhibit serum protein adsorption during blood circulation and promote HA receptor-mediated endocytosis for selective metastatic cancer cells. The hydrodynamic sizes of resulting nano-drugs at 100–200 nm allow prolonged circulation. The developed nano-drugs degrade into less than 10 nm fragments in the glutathione-rich cytosol of cancer cells by cleaving diselenide crosslinkers, allowing intensive gene release and complete urinary excretion. Real-time tracks of release profiles are developed using fluorescence quenching technique. On the other hand, to eliminate premature release, DOX is conjugated to microgel carriers through various linkers for efficiently controlled spatiotemporal release in cancer lysosomes, such as acid-responsive hydrazone linkers, glutathione-cleavable diselenide linkers and enzyme-cleavable peptide linkers. The system comprising of DOX with a hydrazone bond demonstrates 4.4-fold higher in vitro anticancer performance on MDA-MB-231 cell line than free DOX. The nano-drug system of miR-21i and conjugated DOX via hydrazone and diselenide dual bonds completely surpasses DOX premature leakage in physiological condition and results in a high survival rate of over 95 % for HEK293T kidney cell line at a DOX concentration of 2.5 μg mL−1, but it reveals 3.2-fold increase in therapeutic effect on the multidrug-resistant cancer cell line of MDA-MB-231-R12w compared with free DOX. To simplify DOX loading process, diselenide crosslinkers can be used for both microgel synthesis and DOX conjugation to produce a simultaneous release system of gene and DOX in cytosol. The introduction of ATP aptamer to the miR-21i and DOX co-delivery system further increases intracellular DOX accumulation in MDA-MD-231-R12w cells with 4.2-fold higher anticancer performance than free DOX but with low cytotoxicity to healthy cells. To achieve sequential release, the nano-drug system of miR-21i and conjugated DOX with a caspase-3-cleavable DEVDC peptide linker enables the step release of miR-21i followed by DOX. Compared with free DOX, this systems demonstrates a 7.3-fold increase in anticancer effect on MDA-MB-231-R24w and negligible side effects. In summary, we have developed various smart nano-drugs with outstanding advantages of low cytotoxicity, complete biodegradability, targeting and selective delivery and high anticancer performance to MDR cells. Our approach suppresses traditional gene and drug delivery concepts, and advances knowledge for successive design of delivery systems in clinical applications.
Thesis (Ph.D.) -- University of Adelaide, School of Chemical Engineering and Advanced Materials, 2019

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16

Montange, Rebecca Kathryn. "The structure and function of the SAM-I riboswitch aptamer domain." Thesis, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3354542.

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17

Fu, Wei Shan, and 傅韋姍. "Characterization of DNA Aptamers with Selective Binding to Activated Platelets." Thesis, 2019. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107CGU05114090%22.&searchmode=basic.

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18

Scanlan, Mary Smalley. "Fluorescence investigations of RNA folding : group I intron P4-P6 domain and xpt G-riboswitch aptamer domain /." 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3270018.

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Thesis (Ph. D.)--University of Illinois at Urbana-Champaign, 2007.
Source: Dissertation Abstracts International, Volume: 68-06, Section: B, page: 3796. Adviser: Scott K. Silverman. Includes bibliographical references. Available on microfilm from Pro Quest Information and Learning.

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19

Cheng, Kai Wen, and 鄭凱文. "Identification of Aptamer Targeting Coagulation Factor XIII Using Systematic Evolution of Ligands by Exponential Enrichment Technology." Thesis, 2019. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107CGU05114075%22.&searchmode=basic.

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20

Mazumdar, Debapriya. "Investigation of metal-dependence in DNAzymes and applications of DNAzymes and aptamers for diagnostics /." 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3363033.

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Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009.
Source: Dissertation Abstracts International, Volume: 70-06, Section: B, page: 3492. Adviser: Yi Lu. Includes bibliographical references. Available on microfilm from Pro Quest Information and Learning.

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