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Journal articles on the topic "Arcobacter"

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HOUF, KURT, LIEVEN DE ZUTTER, JAN VAN HOOF, and PETER VANDAMME. "Occurrence and Distribution of Arcobacter Species in Poultry Processing." Journal of Food Protection 65, no. 8 (August 1, 2002): 1233–39. http://dx.doi.org/10.4315/0362-028x-65.8.1233.

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A total of 16 broiler flocks slaughtered in the morning in eight Belgian poultry slaughterhouses were examined for the presence of Campylobacteraceae. In samples collected before and after chilling, the prevalence of arcobacters was found to be higher than the prevalence of thermophilic campylobacters, with the slaughter procedure used having no clear effect. Two slaughterhouses were selected for a detailed investigation of the occurrence and distribution of arcobacters. Sampling carried out before slaughter revealed that both Arcobacter butzleri and Arcobacter cryaerophilus were commonly present on the slaughter equipment in both plants. These findings indicate inadequate decontamination of the slaughterhouse environment and suggest potential Arcobacter contamination of broiler carcasses through the slaughter equipment. Even before evisceration, contamination levels of hundreds to several thousands of arcobacters per gram of neck skin were detected. It appears unlikely that contamination through slaughter equipment alone explains the high contamination levels found for poultry products. Arcobacters were not isolated from the 30 intestinal tracts sampled for each broiler flock examined. A. cryaerophilus was the only Arcobacter species recovered from the transport crate samples collected before and after washing. Arcobacter contamination during slaughter, either direct (from chicken intestinal content or feces) or indirect (from equipment), was not confirmed. The origin and the precise routes of contamination remain to be determined.
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Miller, William G., Emma Yee, and James L. Bono. "Complete Genome Sequencing of Four Arcobacter Species Reveals a Diverse Suite of Mobile Elements." Genome Biology and Evolution 12, no. 2 (February 1, 2020): 3850–56. http://dx.doi.org/10.1093/gbe/evaa014.

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Abstract Arcobacter species are recovered from a wide variety of sources, including animals, food, and both fresh and marine waters. Several Arcobacter species have also been recovered from human clinical samples and are thus associated tentatively with food- and water-borne human illnesses. Genome sequencing of the poultry isolate Arcobacter cibarius H743 and the Arcobacter acticola, Arcobacter pacificus, and Arcobacter porcinus type strains identified a large number and variety of insertion sequences. This study presents an analysis of these A. acticola, A. cibarius, A. pacificus, and A. porcinus IS elements. The four genomes sequenced here contain 276 complete and degenerate IS elements, representing 13 of the current 29 prokaryotic IS element families. Expansion of the analysis to include 15 other previously sequenced Arcobacter spp. added 73 complete and degenerate IS elements. Several of these IS elements were identified in two or more Arcobacter species, suggesting movement by horizontal gene transfer between the arcobacters. These IS elements are putatively associated with intragenomic deletions and inversions, and tentative movement of antimicrobial resistance genes. The A. cibarius strain H743 megaplasmid contains multiple IS elements common to the chromosome and, unusually, a complete ribosomal RNA locus, indicating that larger scale genomic rearrangements, potentially resulting from IS element-mediated megaplasmid cointegration and resolution may be occurring within A. cibarius and possibly other arcobacters. The presence of such a large and varied suite of mobile elements could have profound effects on Arcobacter biology and evolution.
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CERVENKA, LIBOR, IVA PESKOVA, MARCELA PEJCHALOVA, and JARMILA VYTRASOVA. "Inhibition of Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii by Plant Oil Aromatics." Journal of Food Protection 71, no. 1 (January 1, 2008): 165–69. http://dx.doi.org/10.4315/0362-028x-71.1.165.

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The inhibitory effect of some plant oil aromatics against three strains of Arcobacter butzleri, two strains of Arcobacter cryaerophilus, and one strain of Arcobacter skirrowii was evaluated. When MICs were determined using the broth macrodilution method, cinnamaldehyde was most inhibitory followed by thymol, carvacrol, caffeic acid, tannic acid, and eugenol (P < 0.001). Sublethal concentrations of the three most potent plant oil aromatics also were examined. Overall, cinnamaldehyde was the most bacteriostatic against all arcobacters tested except A. butzleri when these strains were exposed to the MIC25 of this aromatic aldehyde. The bacteriostatic activities of thymol and carvacrol were concentration and species dependent.
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PEJCHALOVÁ, M., E. DOSTALÍKOVÁ, M. SLÁMOVÁ, I. BROŽKOVÁ, and J. VYTŘASOVÁ. "Prevalence and Diversity of Arcobacter spp. in the Czech Republic." Journal of Food Protection 71, no. 4 (April 1, 2008): 719–27. http://dx.doi.org/10.4315/0362-028x-71.4.719.

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The aim of this study was to examine 634 samples of chicken, lamb, pork, beef, fish, samples from the intensive animal industry and from poultry for slaughter, as well as from the domestic breeding of poultry, horses, pigs, and lambs, from surface water, and from clinical samples for the presence of Arcobacter. All the samples were examined with a cultivation method, followed by confirmation by multiplex PCR. The method of multiplex PCR applied directly to a liquid medium after enrichment was applied only to the samples with the highest probability of the presence of arcobacters. Arcobacter spp. were detected in 11.8% of the samples, of which A. butzleri, A. cryaerophilus, and A. skirrowii were found in 6.6, 5.1, and 0.2% of the samples, respectively. The sources of the arcobacters were chicken meat from the retail market, intensive animal production facilities, domestic chicken breeding facilities, lamb raising environments, surface water and wastewater, and beef swabs taken in a meat processing factory. No occurrence of arcobacters was identified in the swabs from slaughter turkeys, ducks, and wild poultry. No arcobacters were found in horse and pig breeding environments, on pork, or on the swabs of fish. Forty-two rectal swabs taken from humans were also free of Arcobacter. Seventeen isolates of Arcobacter were further identified by sequencing the 16S rRNA gene. Varied genotypes were observed among A. butzleri from chicken meat and chicken breeds, and A. cryaerophilus from wastewater and chicken breeds. They were similar to the genotypes present in wastewater, porcine feces, human stool, and human blood obtained from databases. Our results revealed that the chicken meat from the retail market is an important source of arcobacters. Cross-contamination during handling of chicken carcass practices could play a key role in the spread of Arcobacter.
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Červenka, L., I. Zachová, P. Minříková, and J. Vytřasová. "Effect of pH and water activity on the growth of Arcobacter sp. in culture." Czech Journal of Food Sciences 21, No. 6 (November 18, 2011): 203–9. http://dx.doi.org/10.17221/3499-cjfs.

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The effect was studied of pH value and water activity (aw) on the growth of Arcobacter butzleri and Arcobacter cryaerophilus in culture media at 30&deg;C. Various weak organic acids were used to achieve target pH, and different humectants were used to control aw. Generally, the growth of arcobacters was inhibited at medium pH. Compared to propionic, lactic, malic and ascorbic acids (pH 5.5&ndash;5.0), formic, citric and tartaric acids in the pH range of 6.0&ndash;5.5 were more inhibitory to both arcobacter species. Both arcobacter strains were extremely sensitive to broth environment with a<sub>w</sub> values of &lt; 0.980 using NaCl, glycerol and sucrose as humectants. This sensitivity to aw and pH may well be an important constraint for the distribution and survival of Arcobacter sp. in the environment, particularly in foods and food products. &nbsp;
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COLLADO, LUIS, JOSEP GUARRO, and MARIA JOSÉ FIGUERAS. "Prevalence of Arcobacter in Meat and Shellfish." Journal of Food Protection 72, no. 5 (May 1, 2009): 1102–6. http://dx.doi.org/10.4315/0362-028x-72.5.1102.

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Arcobacter is considered an emergent foodborne and waterborne enteropathogen. However, its prevalence in foods of animal origin is only partially known, because most studies have been concentrated on poultry, pork, and beef, and methods applied do not allow identification of all currently accepted Arcobacter species. We investigated the prevalence of Arcobacter in 203 food samples, 119 samples of seven different types of meats and 84 samples of four types of shellfish. Isolates were identified in parallel by using a published multiplex PCR method and a recently described 16S rDNA restriction fragment length polymorphism method that allows all currently accepted Arcobacter species to be characterized. The global prevalence of Arcobacter was 32%; it was highest in clams (5 of 5 samples, 100%) and chicken (9 of 14 samples, 64.3%) followed by pork (9 of 17 samples, 53.0%), mussels (23 of 56 samples, 41.1%), and duck meat (2 of 5 samples, 40.0%). Turkey meat and beef had a similar recovery rate (10 of 30 samples, 33.3%; 5 of 16 samples, 31.3%; respectively), and rabbit meat had the lowest rate (1 of 10 samples, 10.0%). No arcobacters were found in oysters, frozen shrimps, or sausages. This food survey is the first in which five of the seven accepted Arcobacter species have been isolated. Arcobacter butzleri was the most prevalent species (63.0% of isolates) followed by Arcobacter cryaerophilus (26.6%), Arcobacter mytili (4.7%), Arcobacter skirrowii (3.1%), and Arcobacter nitrofigilis (3.1%). Three (4.7%) of the isolates were classified as belonging to three potentially new phylogenetic lines. Our results indicated that Arcobacter species are widely distributed in the food products studied.
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SCULLION, ROISIN, CLARE S. HARRINGTON, and ROBERT H. MADDEN. "Prevalence of Arcobacter spp. in Raw Milk and Retail Raw Meats in Northern Ireland." Journal of Food Protection 69, no. 8 (August 1, 2006): 1986–90. http://dx.doi.org/10.4315/0362-028x-69.8.1986.

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A 1-year study was undertaken to determine the prevalence of Arcobacter spp. in raw milk and retail raw meats on sale in Northern Ireland. Retail raw poultry samples (n = 94), pork samples (n = 101), and beef samples (n = 108) were obtained from supermarkets in Northern Ireland, and raw milk samples (n = 101) were kindly provided by the Milk Research Laboratory, Department of Agriculture and Rural Development, Belfast, Northern Ireland. Presumptive arcobacters were identified by previously described genus-specific and species-specific PCR assays. Arcobacter spp. were found to be common contaminants of retail raw meats and raw milk in Northern Ireland. Poultry meat (62%) had the highest prevalence, but frequent isolations were made from pork (35%), beef (34%), and raw milk (46%). Arcobacter butzleri was the predominant species isolated from retail raw meats and was the only species isolated from raw milk samples. Arcobacter cryaerophilus was detected less frequently, and Arcobacter skirrowii was detected only as a cocontaminant. To our knowledge, this is the first report of Arcobacter spp. prevalence in a diverse range of products of animal origin in Northern Ireland.
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HOUF, KURT, LIEVEN DE ZUTTER, BIEKE VERBEKE, JAN VAN HOOF, and PETER VANDAMME. "Molecular Characterization of Arcobacter Isolates Collected in a Poultry Slaughterhouse." Journal of Food Protection 66, no. 3 (March 1, 2003): 364–69. http://dx.doi.org/10.4315/0362-028x-66.3.364.

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In a poultry slaughterhouse, Arcobacter contamination was examined over a period of 1 week to establish possible routes of contamination. Samples were collected from the slaughter equipment and from processing water before the onset of slaughter and from the first broiler flock slaughtered on each sampling day. Characterization of 1,079 isolates by enterobacterial repetitive intergenic consensus–polymerase chain reaction and a random amplified polymorphic DNA assay resulted in the delineation of 159 Arcobacter butzleri and 139 Arcobacter cryaerophilus types. From almost all 140 neck skin samples collected before and after evisceration, A. butzleri and A. cryaerophilus were isolated simultaneously at contamination levels ranging from 101 to 104 CFU/g. Only six A. butzleri types present in the slaughterhouse environment were also present on the broiler carcasses. None of the A. cryaerophilus genotypes were detected in both the neck skin and the environmental samples. All A. butzleri types isolated from the feather samples were also isolated from broiler neck skin samples. The slaughter equipment was contaminated with arcobacters before the onset of slaughter, but it appeared unlikely that contamination through the slaughter equipment alone explained the high contamination levels on poultry products. Arcobacters were also present in processing water, but types present in water and poultry products were different. Characterization of the Arcobacter isolates did not clarify the routes of transmission, probably because of the extreme heterogeneity among Arcobacter isolates. However, the results obtained in this study brought to light insufficient decontamination at the processing plant involved in the study and confirmed the survival capacity of certain A. butzleri strains.
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ADESIJI, Y. O., A. O. COKER, and J. K. OLOKE. "Detection of Arcobacter in Feces of Healthy Chickens in Osogbo, Nigeria." Journal of Food Protection 74, no. 1 (January 1, 2011): 119–21. http://dx.doi.org/10.4315/0362-028x.jfp-10-231.

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Isolation of arcobacters in foods, with the highest prevalence reported in poultry, has underscored its importance as a potential food safety problem in recent years. To estimate its prevalence in live birds, fresh stool samples from healthy chickens were screened by enrichment and plating on Arcobacter selective medium containing cefoperazone, amphotericin B, and teicoplanin. Of 150 fecal samples, only 2 (1.3%) yielded Arcobacter. Species were identified with fluorescence resonance energy transfer PCR. Isolate no. 21 from a local flock shared 99% identity with the complete genome of A. butzleri RM4018 (CP000361.1). Isolate no. 4 from a layer hen shared 100% identity with a partial 16S rRNA gene sequence of A. cryaerophilus (EF064151.1). The low prevalence of Arcobacter in the fecal samples of healthy chickens concurs with earlier studies suggesting that Arcobacter appears to be a transient colonizer of poultry intestines and therefore might not be the major source of chicken carcass contamination.
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SCULLION, ROISIN, CLARE S. HARRINGTON, and ROBERT H. MADDEN. "A Comparison of Three Methods for the Isolation of Arcobacter spp. from Retail Raw Poultry in Northern Ireland." Journal of Food Protection 67, no. 4 (April 1, 2004): 799–804. http://dx.doi.org/10.4315/0362-028x-67.4.799.

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Recent evidence suggests that arcobacters, especially Arcobacter butzleri, are potential foodborne pathogens, but standardized detection methods have yet to be established. A study was undertaken to determine which of three isolation methods was the most effective for the isolation of Arcobacter spp. from fresh raw poultry. Method 1 was microaerobic and involved a membrane filtration step followed by plating onto blood agar. Method 2 was also microaerobic and involved enrichment and plating media containing a five-antibiotic cocktail. Method 3 was aerobic and was based on enrichment in a charcoal-based broth containing two antibiotics. Retail poultry samples (n = 50) were obtained from supermarkets in Northern Ireland; the European Community license number was recorded to ensure sample diversity. Presumptive arcobacters were identified using genus-speci c and species-speci c primers. Method 1 resulted in the lowest recovery of arcobacters (28% of samples positive). The detection rate for method 2 (68%) was higher than that for method 3 (50%), but the difference was not significant (P &gt; 0.05). Modification of method 3 by plating the enrichment broth at 24 h, as well as at 48 h, increased recovery to 68%. Use of methods 2 and 3 together increased the number of positive samples detected by approximately 25% compared with use of either method alone. A. butzleri was the most commonly isolated species using all methods. Method 3 detected Arcobacter cryaerophilus in more samples (n = 3) than did methods 1 and 2 (n = 1). Arcobacter skirrowii was detected by only method 3 (n = 1). In terms of sensitivity, ease of use, and diversity of species recovered, modified method 3 was the overall method of choice.
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Dissertations / Theses on the topic "Arcobacter"

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Levican, Asenjo Arturo. "Sanitary importance of arcobacter." Doctoral thesis, Universitat Rovira i Virgili, 2013. http://hdl.handle.net/10803/125666.

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The genus Arcobacter, belongs to the family Campylobacteraceae, along with. Some species, are considered emerging pathogens and have been associated with gastrointestinal diseases and bacteraemia in humans and with diarrhoea, mastitis and abortions in animals. Diarrhoea, is the most common clinical presentation in humans. However, still are not fully defined the routes of transmission, the virulence factors present in pathogenic strains have not been determined and the isolation and identification methods are still deficient. All of these factors contribute to a possible underestimation of the sanitary importance of this genus. In this thesis it has been demonstrated the existence of five new species of this genus. In their differentiation, new tools have been used, such as the Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI TOF) Multilocus Phylogenetic Analysis (MLPA) with concatenated 5 genes (rpoB, gyrB, hsp60, gyrA and atpA). Furthermore, the performance of 5 molecular identification methods was compared and none of them was useful for all the strains studied. In this regard, the updating of 16S rRNARFLP method for the identification of all species proved very useful. Moreover, in this thesis was a great diversity of species of this genus in samples of shellfish and sewage. Both matrices have epidemiological importance; however, they have been poorly studied. It was also demonstrated that the use in parallel of direct culturing and post enrichment, as well as the incubation under aerobic and microaerophilic, enhances the recovery and diversity of arcobacters. Regarding the virulence of Arcobacter spp., most of them proved to be potential enteropathogens for man, as they showed the presence of virulence factors and were able to adhere and invade human intestinal cells (Caco-2). Finally, it was demonstrated that Arcobacter may be confused with Campylobacter sp., generating an underestimation of their sanitary importance.
El género Arcobacter incluye especies consideradas patógenos emergentes, ya que se han asociado con patologías gastrointestinales y bacteriemia en humanos y con diarrea, mastitis y abortos en animales. Sin embargo, aún no se ha definido completamente las rutas de transmisión, los factores de virulencia presentes en cepas patogénicas no se han determinado y los métodos de aislamiento e identificación aún son deficientes. Todo esto genera una subestimación de la importancia sanitaria de Arcobacter. En esta tesis doctoral se demostró la existencia de 5 nuevas especies de este género. Para su diferenciación se utilizó herramientas nuevas, tales como el Matrix Assisted Laser desorption Ionization Time of Flight (MALDI TOF) y un Multilocus Phylogenetic Analysis (MLPA) con 5 genes concatenados (rpoB, gyrB, hsp60, atpA y gyrA). Considerando el número de especies incluidas actualmente en el género (n=17), se evaluó si 5 de los métodos moleculares de identificación son todavía útiles para la identificación de las especies para las que se habían definido o si se generan confusiones, demostrrando que todos ellos generan algún error que osciló entre el 16,8% y 67,4% de las cepas estudiadas. En este sentido, la ampliación del método 16S rRNA-RFLP para poder identificar todas las especies resultó ser de gran utilidad. Por otra parte, en esta tesis se observó una gran diversidad de especies de este género en muestras de mariscos y aguas residuales. Ambas matrices han sido poco estudiados a pesar de su importancia epidemiológica. Más aún, se demostró que el uso en paralelo del cultivo por siembra directa y post enriquecimiento, además de incubación en aerobiosis y microaerofilia, favorece la recuperación de una mayor diversidad. Por otra parte, también se demostró que la mayoría de las especies de este género, en especial algunas cepas A. butzleri, A. cryaerophilus, A. skirrowii, A. trophiarum y A. defluvii, son potenciales enteropatógenos para el hombre, ya que presentaron factores de virulencia y fueron capaces de adherir e invadir células intestinales humanas (Caco-2). Por último, se demostró que Arcobacter puede ser confundido con Campylobacter sp., lo que puede contribuir aún más a subestimar su importancia sanitaria.
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Sloane, Julia Yvette. "Molecular epidemiology of Arcobacter in cattle." Thesis, University of Liverpool, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.533924.

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Stoeva, Kalina. "Arcobacter butzleri-genome organisation and pathogenicity." Thesis, University of Edinburgh, 2006. http://hdl.handle.net/1842/14489.

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This study is the first to analyse the genome of A. butzleri NCTC12481. By using one- and two-dimensional Pulsed Field Gel Electrophoresis in combination with Southern hybridisation the A. butzleri genome size was estimated (2.5 Mb) and a physical map was constructed, containing 35 restriction enzyme recognition sequences and 14 gene markers (5 copies of the genes for 16S rRNA and 23S rRNA’ 2 copies for glyA; one for rpoC and rpoB). In the attempt to identify virulence factors of A. butzleri, a homologue of pglF, a gene involved in N-linked protein glycosylation in C. jejuni, was found. Although the genes surrounding pglF in A. butzleri did not reveal a cluster order as in C. jejuni, the intriguing possibility for protein glycosylation in A. butzleri (perhaps involving different mechanisms) remains, since a glycosylated protein was detected by Alcian blue staining of an outer membrane protein extract. Three major proteins of the A. butzleri outer membrane extract were identified as PorA, FlaB and CadF which are known to play a role in pathogenicity in other bacteria. Electron microscopic analysis revealed a polysaccharide capsule on the surface of A. butzleri. In vitro studies of the interaction of A. butzleri with epithelial cultured cells (Caco-2) demonstrated the ability of A. butzleri to attach to and to invade Caco-2 cells. In order to develop a method for transposon mutagenesis of A. butzleri, a Himar1 transposon delivering vector carrying C. jejuni kanamycin resistance gene (aphA type III), was constructed. Attempts to introduce the vector into A. butzleri by electroporation, conjugation or heat shock transformation all failed. The obtained results provide an important foundation for further investigation of this organism and contribute towards broadening the knowledge of this potential foodborne pathogen.
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Collado, González Luis Roberto. "Taxonomy and epidemiology of the genus arcobacter." Doctoral thesis, Universitat Rovira i Virgili, 2010. http://hdl.handle.net/10803/8744.

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El género Arcobacter incluye especies capaces de producir patología gastrointestinal en humanos y aborto, diarrea y mastitis en ganado. Sin embargo se desconoce su prevalencia en el agua y no existen métodos que permitan la identificación de sus 9 especies. En esta tesis hemos desarrollado un protocolo (16S rDNA-RFLP) capaz de diferenciar la mayoría de las especies. Además, demostramos que la presencia de Arcobacter en aguas se correlaciona con la contaminación fecal y que tiene una elevada prevalencia y diversidad genética en el río Llobregat. Sin embargo, estos microorganismos nunca fueron detectadas en al agua potable, lo que demuestra que el tratamiento de potabilización del agua del río es efectivo para su eliminación. Comprobamos que Arcobacter presentan una elevada prevalencia tanto en diferentes tipos de carnes como en mariscos. Finalmente, se han descubierto y descrito 4 especies nuevas para el género: Arcobacter mytili, Arcobacter valdiviensis, Arcobacter defluvii y Arcobacter molluscorum.
The genus Arcobacter includes species associated with human and animal diseases. However, its prevalence in water is unknown and so far do not exist methods able to differentiate the nine accepted species. In this thesis we have developed a molecular identification method (16S rDNA-RFLP) that enables the differentiation of most of species. In addition, we have demonstrated that the presence of Arcobacter in environmental waters correlates with high level of faecal pollution. Theses microorganisms showed a high prevalence in the Llobregat River and a high genetic diversity. However, these microorganisms were never detected from drinking water, which demonstrate that the treatment of the river water is effective for its elimination. Additionally, have been demonstrated the high prevalence of Arcobacter in several types of meat and shellfish. Finally, 4 new species have been discovered and described: Arcobacter mytili, Arcobacter valdiviensis, Arcobacter defluvii y Arcobacter molluscorum.
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Pérez, Cataluña Alba. "Epidemiology and taxogenomics of the genus arcobacter." Doctoral thesis, Universitat Rovira i Virgili, 2018. http://hdl.handle.net/10803/663660.

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El gènere Arcobacter pertany a la família Campylobacteraceae i inclou espècies, considerades patògens emergents ja que son responsables de produir infeccions en humans i animals. Les bactèries d'aquest gènere tenen una àmplia distribució a nivell mundial i es creu que es transmeten pel consum d'aigües o aliments contaminats. Hi ha diverses eines per a l'estudi epidemiològic que ens ajudarien a aclarir aquestes rutes de contagi. No obstant això, els mecanismes d'acció d'aquests bacteris són encara poc coneguts i no hi ha un tractament estandarditzat. El nombre d'espècies del gènere ha augmentat considerablement en els últims anys i l'ús de noves tècniques d'aïllament ha permès trobar d'altres espècies noves. En la present tesi, es va demostrar que l'ús de la tècnica de Multilocus Sequence Typing (MLST) per a l'estudi epidemiològic és poc precisa. A més, es va evidenciar l'elevada resistència a determinats antibiòtics que suggereixen un canvi en l'ús de les teràpies fins ara utilitzades en les infeccions de Arcobacter. També es van descriure un total de 4 noves espècies utilitzant tècniques com l'anàlisi fenotípic i el Multilocus Phylogenetic Analysis (MLPA) mitjançant la concatenació de 5 gens (atpA, gyrA, gyrB, hsp60 i rpoB) i afegint a més la informació extreta dels genomes seqüenciats d'aquestes espècies i de les espècies filogenèticament properes. L'ús dels genomes i la seva comparació i anàlisi filogenètica va evidenciar també que l'espècie A. cryaerophilus està formada per 4 genomovars, les quals representen espècies genòmiques però que no es van poder diferenciar amb proves fenotípiques. Finalment, l'anàlisi filogenètic dels genomes de totes les espècies del gènere, juntament amb el càlcul de diferents índexs genòmics (ANI, isDDH, AAI, POCP i RSCU) va permetre descobrir que el gènere Arcobacter està en realitat format per almenys 7 gèneres, diferenciables genèticament i mitjançant combinació de proves fenotípiques.
El género Arcobacter pertenece a la familia Campylobacteraceae e incluye especies consideradas patógenos emergentes ya que producen infecciones en humanos y animales. Las bacterias de este género presentan una amplia distribución a nivel mundial y se cree que se transmiten por el consumo de aguas o alimentos contaminados. Existen diversas herramientas para el estudio epidemiológico que nos ayudarían a esclarecer estas rutas de contagio. Sin embargo, los mecanismos de acción de estas bacterias son todavía poco conocidos y no existe tratamiento estandarizado. El número de especies del género ha aumentado considerablemente en los últimos años y el uso de nuevas técnicas de aislamiento ha provocado el hallazgo de otras especies nuevas. En esta tesis, se ha demostrado que el uso de la técnica de Multilocus Sequence Typing (MLST) para el estudio epidemiológico es poco precisa. Además, se evidenció la elevada resistencia a determinados antibióticos que sugieren la necesidad de introducir un cambio en el uso de las terapias utilizadas hasta ahora en las infecciones ptoducidas por Arcobacter. También se describieron un total de 4 nuevas especies utilizando técnicas como el análisis fenotípico, el Multilocus Phylogenetic Analysis (MLPA) mediante la concatenación de 5 genes (atpA, gyrA, gyrB, hsp60 y rpoB) y la información extraída de los genomas secuenciados de dichas especies y de las especies filogenéticamente cercanas. El uso de los genomas, su comparación y el análisis filogenético evidenció además que la especie A. cryaerophilus está formada por 4 genomovares, que representan especies genómicas pero que no se pudieron diferenciar fenotípicamente. Finalmente, el análisis filogenético de los genomas de todas las especies del género, junto con el cálculo de diferentes índices genómicos (ANI, isDDH, AAI, POCP y RSCU) permitió descubrir que el género Arcobacter está en realidad formado por al menos 7 géneros, diferenciables genéticamente y mediante combinación de pruebas fenotípicas.
The genus Arcobacter belongs to the family Campylobacteraceae and includes species considered emergent pathogens because they can produce infections in humans and animals. The species of the genus are widely distributed worldwide and the consumption of contaminated food or water is considered the source of the infection. There are several tools for the epidemiological characterization of the strains that could help to clarify the routes of infection. However, the mechanisms of action of these bacteria are still poorly understood and there is no standardized treatment. The number of species of the genus has increased considerably in recent years and the use of new isolation techniques has led to the discovery of other new species. In this thesis, it was demonstrated that the epidemiological analysis using the Multilocus Sequence Typing (MLST) technique is not precise. In addition, the high resistance to certain antibiotics suggested the need for introducing changes in the treatments used in Arcobacter infections. A total of 4 new species were described using phenotypic characterization, Multilocus Phylogenetic Analysis (MLPA) of 5 genes (atpA, gyrA, gyrB, hsp60 and rpoB) and information extracted from the sequenced genomes of these species and the phylogenetically close ones. The use of genomes and their comparison and phylogenetic analysis also showed that the species A. cryaerophilus is composed by 4 genomovars, which represent genomic species that could not be phenotypically differentiated. Finally, the phylogenetic analysis of the genomes of all the species of the genus, together with the calculation of different genomic indexes (ANI, isDDH, AAI, POCP and RSCU) allowed us to discover that the genus Arcobacter is actually formed by at least 7 genera, differentiable genetically and with a combination of phenotypic tests.
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Abu-Halaweh, Marwan, and n/a. "Molecular Methods for Campylobacter and Arcobacter Detection." Griffith University. School of Biomolecular and Biomedical Science, 2005. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20060223.084457.

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Twenty species and six subspecies of the genera Arcobacter and Campylobacter have been described to date. All are Gram-negative, microaerophilic, curved, spiral or S-shaped cells, and are members of the order Campylobacterales, class Epsilonproteobacteria phylum Proteobacteria. Though most members are pathogenic, C. jejuni, C. coli and A. butzleri are the most frequently isolated species from patients suffering from gastrointestinal illness. The current methods for their detection, identification, and differentiation are cumbersome, time consuming and lack specificity. DNA based molecular techniques including real-time Polymerase Chain Reaction (PCR) and Fingerprinting methods Terminal Restriction Fragments Length Polymorphism (T-RFLP) and Ligase Detection Reaction (LDR) have been used in this project to develop rapid detection and identification methods for Campylobacter and Arcobacter species. Five real-time PCR methods were developed which include: (a) rapid detection and identification of Campylobacter species using real-time PCR adjacent hybridisation probes, (b) rapid identification of C. jejuni using SYBR Green I, (c) rapid detection and differentiation of Arcobacter species using adjacent hybridisation probes, (d) rapid detection and differentiation of Arcobacter species and the Campylobacter group (C. coli, C. jejuni, C. lari, C. hyoilei, C. helviticus, C. hyointestinalis, C. insulaenigrae, C lanienae) using melting temperature (Tm) of adjacent hybridisation probes, and (e) a one tube real-time PCR multiplex for the rapid detection and identification of Campylobacter species, C. coli and C. jejuni using a TaqMan Probe, in an iCycler iQTM (BioRad, USA) and Light CyclerTM (Idaho Technology, USA). [Continued ...]
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Abu-Halaweh, Marwan. "Molecular Methods for Campylobacter and Arcobacter Detection." Thesis, Griffith University, 2005. http://hdl.handle.net/10072/367268.

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Twenty species and six subspecies of the genera Arcobacter and Campylobacter have been described to date. All are Gram-negative, microaerophilic, curved, spiral or S-shaped cells, and are members of the order Campylobacterales, class Epsilonproteobacteria phylum Proteobacteria. Though most members are pathogenic, C. jejuni, C. coli and A. butzleri are the most frequently isolated species from patients suffering from gastrointestinal illness. The current methods for their detection, identification, and differentiation are cumbersome, time consuming and lack specificity. DNA based molecular techniques including real-time Polymerase Chain Reaction (PCR) and Fingerprinting methods Terminal Restriction Fragments Length Polymorphism (T-RFLP) and Ligase Detection Reaction (LDR) have been used in this project to develop rapid detection and identification methods for Campylobacter and Arcobacter species. Five real-time PCR methods were developed which include: (a) rapid detection and identification of Campylobacter species using real-time PCR adjacent hybridisation probes, (b) rapid identification of C. jejuni using SYBR Green I, (c) rapid detection and differentiation of Arcobacter species using adjacent hybridisation probes, (d) rapid detection and differentiation of Arcobacter species and the Campylobacter group (C. coli, C. jejuni, C. lari, C. hyoilei, C. helviticus, C. hyointestinalis, C. insulaenigrae, C lanienae) using melting temperature (Tm) of adjacent hybridisation probes, and (e) a one tube real-time PCR multiplex for the rapid detection and identification of Campylobacter species, C. coli and C. jejuni using a TaqMan Probe, in an iCycler iQTM (BioRad, USA) and Light CyclerTM (Idaho Technology, USA). [Continued ...]
Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Biomedical Sciences
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Teschke, Miriam. "Prävalenz von Arcobacter spp. in Puten- und Schweinefleisch aus dem Berliner Einzelhandel und Vergleich von drei kulturellen Arcobacter-Nachweisverfahren /." Berlin : Mbv, 2008. http://d-nb.info/990056414/04.

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Etonsi, Majde Ali. "Studies on Arcobacter species, their isolation and pathogenicity." Thesis, Heriot-Watt University, 2013. http://hdl.handle.net/10399/2692.

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Arcobacter species, a genus previously classified as ‘aerotolerant campylobacters’, have gained consideration as emerging food and water-borne pathogens. Studies increasingly suggest that they are of significance to veterinary public health and agriculture, but their pathogenicity mechanisms and ability to cause disease in animals and humans are not well understood. This project has revealed that only two of eleven Scottish surface water sources were contaminated with Arcobacter (18 %), and this was the first isolation of Arcobacter spp. from surface waters in Scotland. The two Arcobacter water isolates (SW-DL2 and SW-OL2) were shown to be closely related to species of Arcobacter butzleri. The extracellular proteins (ECP) of all strains were positive for gelatinase activity (50-310 units) and caused haemolysis of sheep and chicken erythrocytes. The A. butzleri reference strain D2686 showed poor haemolysis of human erythrocytes. The cadF genes of A. butzleri D2686 and SW-OL2 were cloned and sequenced, and showed high levels of sequence identity with a gene that encodes fibronectin-binding protein in Campylobacter jejuni. All Arcobacter strains in this study showed the ability to adhere to INT-407 cells in vitro and mutagenizing the cadF genes of A. butzleri D2686 and SW-OL2 resulted in a significant reduction in adherence (P >0.01 and P >0.001 respectively). Five putative virulence genes (cadF, ciaB, flaA, flaB and pldA) have been detected in each of the Arcobacter strains studied. cadF mRNA was also shown to be expressed in all strains with higher levels in A. cryaerophilus and SW-OL2 than the other strains. RT-PCR analysis also revealed that expression of the cadF gene was upregulated in all strains on infection of tissue culture cells with significant differences in levels of expression observed.
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Abdelbaqi, Khalil. "Le genre Arcobacter : étude taxonomique, diagnostique et pathogénique." Bordeaux 1, 2007. http://www.theses.fr/2007BOR13532.

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Les Arcobacters sont considérés comme des pathogènes émergents essentiellement en pathologie digestive humaine bien que quelques cas de bactériémie aient été décrits. La principale voie de contamination de l'homme par Arcobacter serait la voie alimentaire par les volailles dont les carcasses contaminées seraient le véhicule potentiel de transmission de cette bactérie à l'homme. Arcobacter butzleri et Arcobacter cryaerophilus sont les espèces le plus souvent isolées dans les échantillons humains, animaux et environnementaux. Ce travail de thèse a permis de mieux appréhender la taxonomie du groupe des Arcobacters à l'aide des séquences de la sous-unité A de l'ADN gyrase, d'identifier les mutations associées à la résistance aux fluoroquinolones et de mettre en place une PCR en temps réel permettant l'identification des différentes espèces d'Arcobacter qui permettra d'évaluer leur prévalence chez l'homme, dans les élevages, dans les viandes animales, dans le lait ainsi que dans leurs différents réservoirs hydrotelluriques. Nous avons aussi étudié l'effet de l'inactivation des sous unités FlaA et FlaB du flagelle d'A. Butzleri dans la virulence de la bactérie et montré que l'adhérence est essentielle pour la réponse proinflammatoire.
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Books on the topic "Arcobacter"

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G, Coote J., Thomas C, Stewart-Tull D. E. S, and Society for Applied Microbiology, eds. Campylobacter, helicobacter and arcobacter. Oxford, UK: Blackwell Science, 2001.

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D. E. S. Stewart Tull, C. Thomas, and John H. Coote. Campylobacter, Helicobacter and Arcobacter. Wiley & Sons, Incorporated, John, 2001.

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Book chapters on the topic "Arcobacter"

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Salas-Massó, Nuria, Alba Perez-Cataluña, Luis Collado, Arturo Levican, and Maria José Figueras. "Arcobacter." In Handbook of Foodborne Diseases, 245–63. Boca Raton : Taylor & Francis, [2019] | Series: Food microbiology series | “A CRC title, part of the Taylor & Francis imprint, a member of the Taylor & Francis Group, the academic division of T&F Informa plc.”: CRC Press, 2018. http://dx.doi.org/10.1201/b22030-23.

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Bhunia, Arun K. "Campylobacter and Arcobacter." In Foodborne Microbial Pathogens, 289–99. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7349-1_16.

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Miller, William G., and Craig T. Parker. "Campylobacter and Arcobacter." In Genomes of Foodborne and Waterborne Pathogens, 49–65. Washington, DC: ASM Press, 2014. http://dx.doi.org/10.1128/9781555816902.ch4.

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Fitzgerald, Collette, and Irving Nachamkin. "Campylobacter and Arcobacter." In Manual of Clinical Microbiology, 998–1012. Washington, DC, USA: ASM Press, 2015. http://dx.doi.org/10.1128/9781555817381.ch56.

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Taylor, Diane E., and Monika Keelan. "Campylobacter and Arcobacter spp." In Principles and Practice of Clinical Bacteriology, 485–502. Chichester, UK: John Wiley & Sons, Ltd, 2006. http://dx.doi.org/10.1002/9780470017968.ch41.

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Lauwers, S., J. Breynaert, R. Van Etterijck, H. Revets, and T. Mets. "Arcobacter Butzleri in the Elderly in Belgium." In Campylobacters, Helicobacters, and Related Organisms, 515–18. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4757-9558-5_97.

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Wesley, Irene V., and William G. Miller. "Arcobacter: an Opportunistic Human Food-Borne Pathogen?" In Emerging Infections 9, 185–212. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555816803.ch9.

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Mohan, H. V., C. Nishanth, G. Leena, S. Wilfred Ruban, and Porteen Kannan. "Arcobacter SP.: An Emerging Foodborne Zoonotic Pathogen." In Applied Food Science and Engineering with Industrial Applications, 17–28. Toronto; New Jersey: Apple Academic Press, 2019.: Apple Academic Press, 2019. http://dx.doi.org/10.1201/9781351048644-2.

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Ferreira, Susana, Mónica Oleastro, and Fernanda Domingues. "Arcobacter spp. in Food Chain-From Culture to Omics." In Foodborne Pathogens and Antibiotic Resistance, 73–117. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2017. http://dx.doi.org/10.1002/9781119139188.ch4.

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Marinescu, Micaela, Anne Collignon, Fabien Squinazi, Renée Derimay, David Woodward, and Hermy Lior. "Two Cases of Persistent Diarrhoea Associated with Arcobacter sp." In Campylobacters, Helicobacters, and Related Organisms, 521–23. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4757-9558-5_99.

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Conference papers on the topic "Arcobacter"

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Patsekin, Valery, Stephen On, Jennifer Sturgis, Euiwon Bae, Bartek P. Rajwa, Aleksandr Aleksandr, and J. Paul Robinson. "Classification of Arcobacter species using variational autoencoders." In Sensing for Agriculture and Food Quality and Safety XI, edited by Moon S. Kim, Byoung-Kwan Cho, and Bryan A. Chin. SPIE, 2019. http://dx.doi.org/10.1117/12.2521722.

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van Driessche, Ellen, Kurt Houf, Jan van Hoof, and Lieven De Zutter. "Faecal shedding of arcobacter species in Belgian pigs." In Fifth International Symposium on the Epidemiology and Control of Foodborn Pathogens in Pork. Iowa State University, Digital Press, 2003. http://dx.doi.org/10.31274/safepork-180809-516.

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González, A., E. Yeres, C. M. Gentil de Farias, Y. Moreno, and M. A. Ferrús. "Presence of Arcobacter spp. contamination in fresh lettuces for human consumption." In Proceedings of the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009). WORLD SCIENTIFIC, 2010. http://dx.doi.org/10.1142/9789814322119_0091.

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González, A., R. González, and M. A. Ferrús. "Antimicrobial susceptibility and quinolone resistance mechanism of Arcobacter butzleri isolates from sewage samples in Spain." In MICROBES IN APPLIED RESEARCH - Current Advances and Challenges. WORLD SCIENTIFIC, 2012. http://dx.doi.org/10.1142/9789814405041_0100.

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Bayas Morejón, Favian, Riveliño Ramón Curay, Israel Goyes, Darwin Núñez, and Katherin Beltrán. "Fluoroquinolonas susceptibility analysis of arcobacter spp isolated of fresh cheese, from the city of guaranda." In MOL2NET 2019, International Conference on Multidisciplinary Sciences, 5th edition. Basel, Switzerland: MDPI, 2019. http://dx.doi.org/10.3390/mol2net-05-06375.

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Oliveira, Maria Gabriela Xavier de, Andrea Micke Moreno, Maria Garcia Spindola, Vasco Túlio de Moura Gomes, Pedro Henrique de Lima Filsner, Thaís Sebastiana Porfida Ferreira, and Terezinha Knöbl. "Detecção de Cepas Virulentas de Arcobacter Butzleri em Carnes de Frangos e Suínos Provenientes de Açougues no Município de São Paulo." In XII Latin American Congress on Food Microbiology and Hygiene. São Paulo: Editora Edgard Blücher, 2014. http://dx.doi.org/10.5151/foodsci-microal-172.

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BUITVIDAS, CAMILA. "A IMPORTÃNCIA DO CONTROLE SANITÁRIO E A QUALIDADE DA ÁGUA DOS ANIMAIS EM CATIVEIRO." In I Congresso Nacional de Especialidades Veterinárias On-line. Revista Multidisciplinar em Saúde, 2022. http://dx.doi.org/10.51161/convesp/5654.

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Introdução: A água é um importante veículo de transmissão de doenças, presente em todos os ecossistemas. Ao contrário de quando se encontra na natureza, quando em contato com animais de hábitos aquáticos ou semi-aquáticos esta precisa ser tratada, substituída e descartada. Em casos em que não há o manejo sanitário correto, os ambientes podem ter presença de bactérias com importância clínica (Arcobacter spp, Campylobacter spp., Escherichia coli), principalmente porque alguns animais são reservatórios de tais bactérias. Sendo assim, é necessário realizar o controle e diagnóstico microbiológico da água. Objetivo: realizar uma revisão de literatura sobre o risco de contaminação da água utilizada em ambientes de animais com hábitos aquáticos, e o destino dela. Pontuar o tratamento de dejetos, verificar a presença de bactérias de importância para a saúde pública e se representa algum risco para a população. Material e Métodos: Os materiais utilizados como referências bibliográficas e metodologia, foram livros e artigos científicos. Resultados: A introdução de doenças, tanto para os animais de zoológico como para os tratadores, precisa ser uma preocupação constante do médico veterinário que trabalha no local, porque é onde animais selvagens de diferentes regiões encontram-se confinados em uma área relativamente pequena. Quando falamos em biossegurança e manejo sanitário em zoológicos, as limitações orçamentárias dificultam a implementação de ações, pois a todo o momento aparecem outras prioridades. Durante a pesquisa, foi encontrada certa dificuldade em coletar referenciais bibliográficos sobre animais com hábitos totalmente aquáticos presentes em aquários ou Zoológicos do Brasil. Contudo, é possível encontrar diversas normas que auxiliam no tratamento, tanto da água como dos dejetos em geral. Conclusão: Também é possível concluir que, mesmo havendo controle de manejo sanitário e o acompanhamento diário de médicos veterinários, ainda há chances de contaminação, pelo fato de que é muito difícil controlar um ambiente tão aberto como as fundações/parques zoológicos, que recebem visitas de animais de fora, que não passaram por tratamento, como roedores, aves e primatas. E estes, carregam bactérias naturais em sua microbiota que, ao entrar em contato com a água, contaminam-na.
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