Relevant bibliographies by topics / Arabidopsis

Academic literature on the topic 'Arabidopsis'

Author: Grafiati
Published: 4 June 2021
Last updated: 31 July 2024

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Journal articles on the topic "Arabidopsis"

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Lv, Junli, Wei Wu, Tao Ma, Bohan Yang, Asaf Khan, Peining Fu, and Jiang Lu. "Kinase Inhibitor VvBKI1 Interacts with Ascorbate Peroxidase VvAPX1 Promoting Plant Resistance to Oomycetes." International Journal of Molecular Sciences 24, no. 6 (March 7, 2023): 5106. http://dx.doi.org/10.3390/ijms24065106.

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Downy mildew caused by oomycete pathogen Plasmopara viticola is a devastating disease of grapevine. P. viticola secretes an array of RXLR effectors to enhance virulence. One of these effectors, PvRXLR131, has been reported to interact with grape (Vitis vinifera) BRI1 kinase inhibitor (VvBKI1). BKI1 is conserved in Nicotiana benthamiana and Arabidopsis thaliana. However, the role of VvBKI1 in plant immunity is unknown. Here, we found transient expression of VvBKI1 in grapevine and N. benthamiana increased its resistance to P. viticola and Phytophthora capsici, respectively. Furthermore, ectopic expression of VvBKI1 in Arabidopsis can increase its resistance to downy mildew caused by Hyaloperonospora arabidopsidis. Further experiments revealed that VvBKI1 interacts with a cytoplasmic ascorbate peroxidase, VvAPX1, an ROS-scavenging protein. Transient expression of VvAPX1 in grape and N. benthamiana promoted its resistance against P. viticola, and P. capsici. Moreover, VvAPX1 transgenic Arabidopsis is more resistant to H. arabidopsidis. Furthermore, both VvBKI1 and VvAPX1 transgenic Arabidopsis showed an elevated ascorbate peroxidase activity and enhanced disease resistance. In summary, our findings suggest a positive correlation between APX activity and resistance to oomycetes and that this regulatory network is conserved in V. vinifera, N. benthamiana, and A. thaliana.
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Liu, Hang, Hongfei Zhu, Fei Liu, Limiao Deng, Guangxia Wu, Zhongzhi Han, and Longgang Zhao. "From Organelle Morphology to Whole-Plant Phenotyping: A Phenotypic Detection Method Based on Deep Learning." Plants 13, no. 9 (April 23, 2024): 1177. http://dx.doi.org/10.3390/plants13091177.

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The analysis of plant phenotype parameters is closely related to breeding, so plant phenotype research has strong practical significance. This paper used deep learning to classify Arabidopsis thaliana from the macro (plant) to the micro level (organelle). First, the multi-output model identifies Arabidopsis accession lines and regression to predict Arabidopsis’s 22-day growth status. The experimental results showed that the model had excellent performance in identifying Arabidopsis lines, and the model’s classification accuracy was 99.92%. The model also had good performance in predicting plant growth status, and the regression prediction of the model root mean square error (RMSE) was 1.536. Next, a new dataset was obtained by increasing the time interval of Arabidopsis images, and the model’s performance was verified at different time intervals. Finally, the model was applied to classify Arabidopsis organelles to verify the model’s generalizability. Research suggested that deep learning will broaden plant phenotype detection methods. Furthermore, this method will facilitate the design and development of a high-throughput information collection platform for plant phenotypes.
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Deb, Devdutta, Ryan G. Anderson, Theresa How-Yew-Kin, Brett M. Tyler, and John M. McDowell. "Conserved RxLR Effectors From Oomycetes Hyaloperonospora arabidopsidis and Phytophthora sojae Suppress PAMP- and Effector-Triggered Immunity in Diverse Plants." Molecular Plant-Microbe Interactions® 31, no. 3 (March 2018): 374–85. http://dx.doi.org/10.1094/mpmi-07-17-0169-fi.

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Effector proteins are exported to the interior of host cells by diverse plant pathogens. Many oomycete pathogens maintain large families of candidate effector genes, encoding proteins with a secretory leader followed by an RxLR motif. Although most of these genes are very divergent between oomycete species, several genes are conserved between Phytophthora species and Hyaloperonospora arabidopsidis, suggesting that they play important roles in pathogenicity. We describe a pair of conserved effector candidates, HaRxL23 and PsAvh73, from H. arabidopsidis and P. sojae respectively. We show that HaRxL23 is expressed early during infection of Arabidopsis. HaRxL23 triggers an ecotype-specific defense response in Arabidopsis, suggesting that it is recognized by a host surveillance protein. HaRxL23 and PsAvh73 can suppress pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) in Nicotiana benthamiana and effector-triggered immunity (ETI) in soybean. Transgenic Arabidopsis constitutively expressing HaRxL23 or PsAvh73 exhibit suppression of PTI and enhancement of bacterial and oomycete virulence. Together, our experiments demonstrate that these conserved oomycete RxLR effectors suppress PTI and ETI across diverse plant species.
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Shigemori, Hideyuki, Haruyuki Nakajyo, Yosuke Hisamatsu, Mitsuhiro Sekiguchi, Nobuharu Goto, and Koji Hasegawa. "Arabidopside F, a New Oxylipin from Arabidopsis thaliana." HETEROCYCLES 69, no. 1 (2006): 295. http://dx.doi.org/10.3987/com-06-s(o)33.

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Abe, Hiroshi, Jun Ohnishi, Mari Narusaka, Shigemi Seo, Yoshihiro Narusaka, Shinya Tsuda, and Masatomo Kobayashi. "Arabidopsis." Plant Signaling & Behavior 3, no. 7 (July 2008): 446–47. http://dx.doi.org/10.4161/psb.3.7.5556.

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Hisamatsu, Yosuke, Nobuharu Goto, Koji Hasegawa, and Hideyuki Shigemori. "Senescence-Promoting Effect of Arabidopside A." Zeitschrift für Naturforschung C 61, no. 5-6 (June 1, 2006): 363–66. http://dx.doi.org/10.1515/znc-2006-5-611.

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Arabidopside A isolated from Arabidopsis thaliana is a rare oxylipin, containing 12-oxophytodienoic acid (OPDA) and dinor-oxophytodienoic acid (dn-OPDA) which are known as precursors of jasmonic acid (JA) and methyl jasmonate (MeJA). The senescence-promoting effect of arabidopside A was examined by an oat (Avena sativa) leaf assay under dark or continuous light condition. Arabidopside A promoted senescence of oat leaves, and the promoting activity was more effective than for JA and OPDA, and as strong as for MeJA, which was well known to be a senescence promoter. These results suggest that arabidopside A plays important roles in leaf senescence.
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Murray, J., J. Larsen, T. E. Michaels, A. Schaafsma, C. E. Vallejos, and K. P. Pauls. "Identification of putative genes in bean (Phaseolus vulgaris) genomic (Bng) RFLP clones and their conversion to STSs." Genome 45, no. 6 (December 1, 2002): 1013–24. http://dx.doi.org/10.1139/g02-069.

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A set of 79 previously mapped bean (Phaseolus vulgaris) genomic (Bng) clones were partially sequenced. BLAST database searches detected homologies between 59 of these clones and genes from a variety of plants, especially Arabidopsis thaliana. Some matches in the database to the Bng clones included a putative P-glycoprotein – ABC transporter from Arabidopsis, an early nodulin-binding protein (ENBP1) from Medicago truncatula, a lon-protease protein from spinach, a branched-chain amino-acid aminotransferase from Arabidopis, and a vacuolar sorting receptor (BP-80) from Pisum sativum. Additional matches were found for genes involved in isoprenoid biosynthesis, sulfur metabolism, proline biosynthesis, and floral development. Sequence tagged site (STSs) were produced for 16 of the clones, 2 of which contain simple sequence repeats (SSRs). Polymorphisms were detected for six of the STSs.Key words: CAPS, SSR, molecular markers, gene identification.
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Mohr, Toni J., Nicole D. Mammarella, Troy Hoff, Bonnie J. Woffenden, John G. Jelesko, and John M. McDowell. "The Arabidopsis Downy Mildew Resistance Gene RPP8 Is Induced by Pathogens and Salicylic Acid and Is Regulated by W Box cis Elements." Molecular Plant-Microbe Interactions® 23, no. 10 (October 2010): 1303–15. http://dx.doi.org/10.1094/mpmi-01-10-0022.

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Plants disease resistance (R) genes encode specialized receptors that are quantitative, rate-limiting defense regulators. R genes must be expressed at optimum levels to function properly. If expression is too low, downstream defense responses are not activated efficiently. Conversely, overexpression of R genes can trigger autoactivation of defenses with deleterious consequences for the plant. Little is known about R gene regulation, particularly under defense-inducing conditions. We examined regulation of the Arabidopsis thaliana gene RPP8 (resistance to Hyaloperonospora arabidopsidis, isolate Emco5). RPP8 was induced in response to challenge with H. arabidopsidis or application of salicylic acid, as shown with RPP8-Luciferase transgenic plants and quantitative reverse-transcription polymerase chain reaction of endogenous alleles. The RPP1 and RPP4 genes were also induced by H. arabidopsidis and salicylic acid, suggesting that some RPP genes are subject to feedback amplification. The RPP8 promoter contains three W box cis elements. Site-directed mutagenesis of all three W boxes greatly diminished RPP8 basal expression, inducibility, and resistance in transgenic plants. Motif searches indicated that the W box is the only known cis element that is statistically overrepresented in Arabidopsis nucleotide-binding leucine-rich repeat promoters. These results indicate that WRKY transcription factors can regulate expression of surveillance genes at the top of the defense-signaling cascade.
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Akimov, Yu. "Ultrastructure of mesophyll cells of Arabidopsis (Arabidopsis thaliana L.) after hyperthermia." Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology 85, no. 2 (2021): 15–22. http://dx.doi.org/10.17721/1728_2748.2021.85.15-22.

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The influence of hyperthermia (33 ºC, 2 days) on the ultrastructure of palisade cells of mesophyll of the first rosette leaves of arabidopsis Columbia 0 ecotype (Col-0, phases 1.02–1.04) was studied. Samples of 12-day-old seedlings were selected in 2 variants: control and 2 days 33 ºC. Seedlings of the control variant were grown in a growth chamber with a photoperiod of 15/9 hours. (day/night), illumination 5.5 klx, 75 % humidity and temperature 22 ºC. In the experimental variant containers with 9-day-old seedlings were transferred for 2 days to a growth chamber with a preset light 5.5 klx and temperature 33 ºC, with a photoperiod of 15/9 hours. The conducted ultrastructural analysis allowed to reveal the spectrum of rearrangements of palisade cells after two-day action of high (33 ºC) temperature. It was shown that the high temperature negatively affected size of mesophyll palisade cells, the cross-sectional area of which was 12 % smaller than in the control. Chloroplasts show an increase in granality: in the control granas contained 6–10 thylakoids, often combining into larger granas, up to 20 or more thylakoids in the intersection zone, while after two-day hyperthermia the granas contained 20 or more thylakoids, often forming giant granas of 60 and more thylakoids, the average cross-sectional area of starch granules decreased by almost half: 0.99 μm2 compared to 1.92 μm2 in the control, the diameter of plastoglobuli increased 3–4 times: to 100–200 nm compared to 30–50 nm in the control. In mitochondria, there was a decrease in the partial volume of the cristae, enlightenment of the matrix, the cross-section of mitochondria increased at least twice: 1 μm2 compared to 0.44 μm2 in the control. The mean cross-sectional area of peroxisomes also increased at least twice, to 1.36 μm2 compared with 0.77 μm2 in the control.
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Somerville, Chris. "Arabidopsis Blooms." Plant Cell 1, no. 12 (December 1989): 1131. http://dx.doi.org/10.2307/3868910.

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Dissertations / Theses on the topic "Arabidopsis"

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Tasker, Adam. "Pectinesterase in Arabidopsis." Thesis, University of Nottingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.523497.

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Bano, Aziz Fatima. "Glucosinolates in Arabidopsis." Thesis, University of East Anglia, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.262028.

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Pavangadkar, Kanchan Amol. "Role of ADA2b and GCN5 in COR gene expression during cold acclimation in Arabidopsis." Diss., Connect to online resource - MSU authorized users, 2008.

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Morris, Erin Rebecca. "FHA domain genes of Arabidopsis /." free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p3144443.

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Coker, Timothy L. R. "Infection-site-specific responses of Arabidopsis thaliana to the biotrophic oomycete Hyaloperonospora arabidopsidis." Thesis, University of Warwick, 2016. http://wrap.warwick.ac.uk/90202/.

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Changes in gene expression form a crucial part of the plant response to infection, and whole-leaf expression profiling has been valuable in our understanding of the interactions between the model plant Arabidopsis thaliana and a diverse range of pathogens. However, when studying the interaction between Arabidopsis and the biotrophic oomycete Hyaloperonospora arabidopsidis (Hpa), whole-leaf profiling may fail to capture the complete Arabidopsis response. Highly localised expression changes that occur in infected cells may be diluted by the comparative abundance of non-infected cells, or local and systemic responses of a differing nature may become convoluted. The aim of this project was to spatially dissect the transcriptional response to Hpa, allowing differentiation of local and more systemic responses. Fluorescence Activated Cell Sorting (FACS), utilising an infection-site-specific fluorescent marker ProDMR6::GFP, was performed in order to isolate Hpa-proximal and Hpa-distal cells from infected seedling samples, and global gene expression measured in these FACS-sorted samples using microarrays. When compared with an uninfected control, 278 transcripts were identified as differentially expressed, the vast majority of which were differentially expressed specifically in Hpa-proximal cells. By comparing our data to previous, whole organ studies, we discovered many locally responding genes that were detected for the first time using our sensitive FACS technique. A portion of locally-responding genes were selected for further study. The promoters of a subset of highly locally induced genes were selected to drive expression of Green Fluorescent Protein (GFP) as a marker of Hpa-contacting cells for further FACS experiments, and to further validate their localised induction. Although some evidence of localised induction was seen in these lines, further investigation is required. We also hypothesised that a number of locally-induced genes would have a functional influence on infection, and tested this through the use of genetic knockouts. Knockouts in 7 of these genes showed altered disease resistance or susceptibility, and the mechanism behind two of these genes was investigated through the use of microarrays. Overall, the use of FACS to study the Arabidopsis response to Hpa on a spatial scale has allowed identification of new genes with a putative role in the Arabidopsis-Hpa interaction, and contributes to a systems-level understanding of plant-pathogen interactions.
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Dinneny, Jose R. "Patterning organs in Arabidopsis /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2005. http://wwwlib.umi.com/cr/ucsd/fullcit?p3190169.

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Strachan, Camille. "Phosphoproteomics of Arabidopsis thaliana." [Gainesville, Fla.] : University of Florida, 2005. http://purl.fcla.edu/fcla/etd/UFE0011590.

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Usher, Sarah Louise. "Nucleosome positioning in Arabidopsis." Thesis, University of Warwick, 2009. http://wrap.warwick.ac.uk/3212/.

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The aim of this project was to test hypotheses relating to nucleosome positioning in Arabidopsis to provide a basis for better understanding of epigenetic transcriptional regulation in plants. Prior to this study, virtually no information existed regarding nucleosome positioning in plants. Eukaryote chromosomes consist of chromatin, composed of nucleosomes separated by linker DNA of variable lengths. Nucleosomes consist of 147 bp of DNA wrapped 1.7 times around a histone octamer. Whilst no consensus nucleosome positioning DNA sequence exists, sequence preferences influence positioning, and contribute to the complex epigenetic processes which act to control transcriptional activity. These details of the underlying mechanisms are known to differ between the plant and animal kingdoms. High-throughput sequencing technologies were utilised to generate large datasets of mono- and di-nucleosome sequences from wild-type Arabidopsis. These enabled genome-wide analysis and inference of plant-specific patterns of nucleosome positioning and sequence properties. Further data were generated from a methyltransferase antisense (MET1) which is depleted in methylated CG epigenetic marks. The internal distributions of dinucleotides within Arabidopsis nucleosomes were similar to those observed in non-plant eukaryotes. A unique periodicity in the distribution of linker lengths was detected in Arabidopsis wild type chromatin. In contrast, the MET1 antisense line displayed the expected periodicity, indicating systematic differences in chromatin organisation. There was a significant increase in nucleosome occupancy within exons compared with introns. However, this difference was less marked in the MET1 antisense. Specific patterns of nucleosome phasing were observed around transcription start sites. Linker lengths within rRNA gene clusters associated with nucleolar organiser regions (NORs) differed depending on chromosome of origin, suggesting differences in higher order chromatin structure between the NORs. Comparison of the nucleosome position and DNA methylation within the rRNA gene cluster revealed interesting differences between the two regions, which may reflect interactions affecting chromatin structure and transcriptional regulation.
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Zhang, Yi. "Wound responses in arabidopsis." Thesis, University of East Anglia, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.502215.

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Environmental stresses, such as repeated injury by herbivory, stunt plant growth and reduce crop yield. A spectacular example of this effect is exemplified in ornamental bonsai plants. Wounding induces the synthesis of a plant hormone, jasmonates (JAs), which in turn activate non-specific defence against pests and pathogens. On the other hand, a most dramatic effect of the application of jasmonates to plant however is the inhibition of growth, and this raised the question of whether another function of endogenous jasmonates is to inhibit growth. The results presented in this thesis first demonstrated that a previous wounding primes plants to give an enhanced response to following wounds. Following this discovery, I have investigated the genetic and physiological basis of "bonsai effect" by repeatedly wounding leaves of the model plant Arabidopsis. Repeated wounding reduced the size of wild type plants by 50% and increased the endogenous content of jasmonate (JA) by seven-fold, but unexpectedly had no significant effect on the mutants unable to synthesise JA, or unable to respond to JA. This second discovery suggests another function of endogenous JA is to inhibit growth under stress.
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Caryl, A. P. "Gene tagging in Arabidopsis." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597347.

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The work described in this thesis takes two approaches towards the identification of tagged genes in Arabidopsis thaliana. A family screening approach was used to isolate mutants from the T-DNA transformed Feldmann lines of Arabidopsis. Co-segregation analysis was used to determine whether these mutants were tagged. None of the mutants isolated showed co-segregation with the T-DNA so this approach was abandoned. Enhancer trap constructs are designed to report the activity of enhancers near a transgene insert. Typically they contain a reporter gene with an weak promoter in a transformation vector. The second approach used in this study was to investigate a collection of 123 independently transformed lines of Arabidopsis containing such constructs which were available in the laboratory. The construct introduced into these lines contained a β-Glucuronidase reporter gene under the control of an attenuated Cauliflower Mosaic Virus promoter. The number of independent active inserts, T-DNA copy number and GUS staining patterns of the lines were investigated. One of the lines, Δ31, showed GUS staining associated with meristematic tissue (with the exception of the primary root meristem). The expression of the GUS reporter gene was presumably being controlled by a plant enhancer adjacent to the insert. Presumably the enhancer would normally act to regulate the expression of an endongenous plant gene. Spectrophotometric assays were used to measure the response to auxin in root tissues. IPCR was used to amplify plant DNA flanking the insert in line Δ31. A clone of this DNA was used to isolate four large overlapping clones from a lambda genomic library of wild-type plants which would be likely to contain the tagged enhancer, and any endogenous gene regulated by this enhancer.
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Books on the topic "Arabidopsis"

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Bowman, John, ed. Arabidopsis. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4612-2598-0.

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M, Meyerowitz Elliot, and Somerville Chris R, eds. Arabidopsis. Plainview , N.Y: Cold Spring Harbor Laboratory Press, 1994.

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Mary, Anderson, and Roberts J. A, eds. Arabidopsis. Sheffield, England: Sheffield Academic Press, 1998.

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Martinez-Zapater, José M., and Julio Salinas, eds. Arabidopsis Protocols. Totowa, NJ: Humana Press, 1998. http://dx.doi.org/10.1385/0896033910.

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Julio, Salinas, and Sanchez-Serrano J. Jose. Arabidopsis Protocols. New Jersey: Humana Press, 2006. http://dx.doi.org/10.1385/1597450030.

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Sanchez-Serrano, Jose J., and Julio Salinas, eds. Arabidopsis Protocols. Totowa, NJ: Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-580-4.

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Sanchez-Serrano, Jose J., and Julio Salinas, eds. Arabidopsis Protocols. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-0880-7.

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J, Sánchez-Serrano Jose, ed. Arabidopsis protocols. New York: Humana Press, 2014.

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M, Martínez-Zapater José, and Salinas Julio, eds. Arabidopsis protocols. Totowa, N.J: Humana Press, 1998.

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Julio, Salinas, and Sánchez-Serrano Jose J, eds. Arabidopsis protocols. 2nd ed. Totowa, N.J: Humana Press, 2005.

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Book chapters on the topic "Arabidopsis"

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Bowman, John, J. D. Callos, F. J. Behringer, J. Vasinda, D. Stewart, B. M. Link, J. I. Medford, et al. "Vegetative Development." In Arabidopsis, 1–89. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4612-2598-0_1.

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Dolan, L., P. Linstead, K. Roberts, T. I. Baskin, R. Williamson, P. Benfey, J. W. Schiefelbein, K. Okada, and Y. Shimura. "Roots." In Arabidopsis, 91–131. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4612-2598-0_2.

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Bowman, J. L., D. R. Smyth, J. P. Hill, E. M. Lord, S. Craig, A. Chaudhary, A. R. Davis, et al. "Flowers." In Arabidopsis, 133–273. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4612-2598-0_3.

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Bowman, J. L., J. Dawson, Z. A. Wilson, L. G. Briarty, B. J. Mullingan, S. Craig, and A. Chaudhury. "Pollen." In Arabidopsis, 275–95. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4612-2598-0_4.

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Bowman, J. L., S. G. Mansfield, Z. Modrusan, L. Reiser, R. L. Fischer, G. W. Haughn, K. A. Feldman, and M. C. Webb. "Ovules." In Arabidopsis, 297–331. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4612-2598-0_5.

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Bowman, J. L., M. C. Webb, S. Craig, and A. Chaudhury. "Pollination." In Arabidopsis, 333–47. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4612-2598-0_6.

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Bowman, J. L., S. G. Mansfield, and M. Koorneef. "Embryogenesis." In Arabidopsis, 349–401. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4612-2598-0_7.

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Dangl, J., H. Liedgens, T. Debener, B. Mauch-Mani, A. J. Slusarenko, F. M. W. Grundler, U. Wyss, and W. Golinowski. "Pathogens." In Arabidopsis, 403–23. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4612-2598-0_8.

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Katam, Ramesh, Dilip R. Panthee, Anjanabha Bhattacharya, Sheikh M. Basha, and Chittaranjan Kole. "Arabidopsis." In Wild Crop Relatives: Genomic and Breeding Resources, 1–16. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-14871-2_1.

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Scholl, Randy, Luz Rivero-Lepickas, and Deborah Crist. "Growth of Plants and Preservation of Seeds." In Arabidopsis Protocols, 1–12. Totowa, NJ: Humana Press, 1998. http://dx.doi.org/10.1385/0-89603-391-0:1.

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Conference papers on the topic "Arabidopsis"

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"Expression of IPD3, a transcriptional regulator of AM symbiosis, affects immunity and flowering time in non-host Arabidopsis." In IS-MPMI Congress. IS-MPMI, 2023. http://dx.doi.org/10.1094/ismpmi-2023-13.

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Arbuscular mycorrhizal symbiosis (AM) is a beneficial trait originating with the first land plants. The ability to host AM has since been lost from diverse plant species. Genes in the Common Symbiosis Pathway that are essential to establish AM hosting were lost from Brassicaceae along with the trait itself, including Interacting Protein of DMI3 (IPD3), a key transcription factor connecting upstream signaling of AM fungal presence to the downstream gene-regulatory network for AM functions. We generated transgenic Arabidopsis plants expressing the DNA-binding domain of IPD3 and used phenotypic and transcriptome analysis to characterize the effect of IPD3 expression in Arabidopsis in the presence and absence of AM fungus Rhizophagus irregularis. We compared these results to the AM-host model Lotus japonicus and its ipd3 knockout mutant cyclops-4. Despite its long history as a non-AM species, restoring IPD3 expression to Arabidopsis significantly altered transcription related to growth and defense, and delayed flowering time. Our comparative transcriptomic results indicate that some genetic connections for IPD3 remain conserved in Arabidopsis despite the long evolutionary time since its loss.
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Ji, Guoli, Shanshan Wu, Xiaohui Wu, Denghui Xing, and Qingshun Quinn Li. "Data Analysis of Arabidopsis Tiling Array." In 2009 First International Conference on Information Science and Engineering. IEEE, 2009. http://dx.doi.org/10.1109/icise.2009.444.

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Euler Angelo de Menezes Junior and Fabrício Martins Lopes. "Integração de Dados da Arabidopsis thaliana." In XX Seminário de Iniciação Científica e Tecnológica da UTFPR. Curitiba, PR, Brasil: Universidade Tecnológica Federal do Paraná - UTFPR, 2015. http://dx.doi.org/10.20906/cps/sicite2015-0484.

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"Adaptation to polyploidy in Siberian Arabidopsis lyrata." In Bioinformatics of Genome Regulation and Structure/Systems Biology (BGRS/SB-2022) :. Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, 2022. http://dx.doi.org/10.18699/sbb-2022-356.

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Kurimoto, T., J. V. H. Constable, S. Hood, A. Huda, Carlos Granja, Claude Leroy, and Ivan Stekl. "Response of Arabidopsis thaliana to Ionizing Radiation." In Nuclear Physics Medthods and Accelerators in Biology and Medicine. AIP, 2007. http://dx.doi.org/10.1063/1.2825822.

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Swartz, Landon G., Suxing Liu, David Mendoza Cozatl, and Kannappan Palaniappan. "Segmentation of Arabidopsis thaliana Using Segment-Anything." In 2023 IEEE Applied Imagery Pattern Recognition Workshop (AIPR). IEEE, 2023. http://dx.doi.org/10.1109/aipr60534.2023.10440688.

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Hassan, Sayyeda Hira, Gabriella Sferra, Daniele Fantozzi, Melissa Simiele, Gabriella Stefania Scippa, Domenico Morabito, and Dalila Trupiano. "Dissecting Protein-Protein Interaction Networks of Arabidopsis thaliana and Arabidopsis halleri to Get Insights into Heavy Metal Tolerance Strategies." In IECPS 2021. Basel Switzerland: MDPI, 2021. http://dx.doi.org/10.3390/iecps2021-11959.

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Boyko, E. V., and I. F. Golovatskaya. "The effect of melatonin and IAA on the growth of Arabidopsis thaliana cotyledons seedlings in different spectral composition of the light." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.049.

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We studied the effect of melatonin and IAA on the growth of Arabidopsis thaliana cotyledons seedlings in red and blue light. The mutual influence of melatonin and IAA on the regulation of cotyledon growth under selective light was shown.
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Xiao Yu, Fanjiang Meng, Xihai Zhang, and Lina Lu. "Mining osmotic stress response genes from Arabidopsis genome." In 2010 International Conference on Image Analysis and Signal Processing. IEEE, 2010. http://dx.doi.org/10.1109/iasp.2010.5476072.

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Li, Eryan, Shudong Wang, Yansen Su, Longxiao Sun, and Dazhi Meng. "Construction and analysis of gene networks for Arabidopsis." In 2011 IEEE International Conference on Signal Processing, Communications and Computing (ICSPCC). IEEE, 2011. http://dx.doi.org/10.1109/icspcc.2011.6061816.

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Reports on the topic "Arabidopsis"

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Britt, Anne. G2 Checkpoint Responses in Arabidopsis. Office of Scientific and Technical Information (OSTI), March 2013. http://dx.doi.org/10.2172/1116357.

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Mas, Paloma. El reloj circadiano de Arabidopsis thaliana. Sociedad Española de Bioquímica y Biología Molecular (SEBBM), June 2015. http://dx.doi.org/10.18567/sebbmdiv_anc.2015.06.1.

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John M. Ward. Functional Analysis of Arabidopsis Sucrose Transporters. Office of Scientific and Technical Information (OSTI), March 2009. http://dx.doi.org/10.2172/950496.

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Anthony R. Cashmore. Light responses in Photoperiodism in Arabidopsis thaliana. Office of Scientific and Technical Information (OSTI), August 2006. http://dx.doi.org/10.2172/893226.

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Fluhr, Robert, and Maor Bar-Peled. Novel Lectin Controls Wound-responses in Arabidopsis. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7697123.bard.

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Innate immune responses in animals and plants involve receptors that recognize microbe-associated molecules. In plants, one set of this defense system is characterized by large families of TIR–nucleotide binding site–leucine-rich repeat (TIR-NBS-LRR) resistance genes. The direct interaction between plant proteins harboring the TIR domain with proteins that transmit and facilitate a signaling pathway has yet to be shown. The Arabidopsis genome encodes TIR-domain containing genes that lack NBS and LRR whose functions are unknown. Here we investigated the functional role of such protein, TLW1 (TIR LECTIN WOUNDRESPONSIVE1). The TLW1 gene encodes a protein with two domains: a TIR domain linked to a lectin-containing domain. Our specific aim in this proposal was to examine the ramifications of the TL1-glycan interaction by; A) The functional characterization of TL1 activity in the context of plant wound response and B) Examine the hypothesis that wounding induced specific polysaccharides and examine them as candidates for TL-1 interactive glycan compounds. The Weizmann group showed TLW1 transcripts are rapidly induced by wounding in a JA-independent pathway and T-DNA-tagged tlw1 mutants that lack TLW1 transcripts, fail to initiate the full systemic wound response. Transcriptome methodology analysis was set up and transcriptome analyses indicates a two-fold reduced level of JA-responsive but not JA-independent transcripts. The TIR domain of TLW1 was found to interact directly with the KAT2/PED1 gene product responsible for the final b-oxidation steps in peroxisomal-basedJA biosynthesis. To identify potential binding target(s) of TL1 in plant wound response, the CCRC group first expressed recombinant TL1 in bacterial cells and optimized conditions for the protein expression. TL1 was most highly expressed in ArcticExpress cell line. Different types of extraction buffers and extraction methods were used to prepare plant extracts for TL1 binding assay. Optimized condition for glycan labeling was determined, and 2-aminobenzamide was used to label plant extracts. Sensitivity of MALDI and LC-MS using standard glycans. THAP (2,4,6- Trihydroxyacetophenone) showed minimal background peaks at positive mode of MALDI, however, it was insensitive with a minimum detection level of 100 ng. Using LC-MS, sensitivity was highly increased enough to detect 30 pmol concentration. However, patterns of total glycans displayed no significant difference between different extraction conditions when samples were separated with Dionex ICS-2000 ion chromatography system. Transgenic plants over-expressing lectin domains were generated to obtain active lectin domain in plant cells. Insertion of the overexpression construct into the plant genome was confirmed by antibiotic selection and genomic DNA PCR. However, RT-PCR analysis was not able to detect increased level of the transcripts. Binding ability of azelaic acid to recombinant TL1. Azelaic acid was detected in GST-TL1 elution fraction, however, DHB matrix has the same mass in background signals, which needs to be further tested on other matrices. The major findings showed the importance of TLW1 in regulating wound response. The findings demonstrate completely novel and unexpected TIR domain interactions and reveal a control nexus and mechanism that contributes to the propagation of wound responses in Arabidopsis. The implications are to our understanding of the function of TIR domains and to the notion that early molecular events occur systemically within minutes of a plant sustaining a wound. A WEB site (http://genome.weizmann.ac.il/hormonometer/) was set up that enables scientists to interact with a collated plant hormone database.
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Meyerowitz, E. Molecular analysis of ethylene-insensitive mutants in arabidopsis. Office of Scientific and Technical Information (OSTI), January 1991. http://dx.doi.org/10.2172/5026591.

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Gibson, Susan I. Characterization of Sugar Insensitive (sis) Mutants of Arabidopsis. Office of Scientific and Technical Information (OSTI), June 2009. http://dx.doi.org/10.2172/956330.

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Vierstra, Richard D. Molecular Dissection of the Arabidopsis 26S Proteasome. Office of Scientific and Technical Information (OSTI), September 2016. http://dx.doi.org/10.2172/1483794.

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Rodermel, Steven. Regulation of chloroplast biogenesis: the immutans mutant of Arabidopsis. Office of Scientific and Technical Information (OSTI), November 2015. http://dx.doi.org/10.2172/1225973.

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Wang, Zhiyong, and Alma Burlingame. Final Report: Proteomic study of brassinosteroid responses in Arabidopsis. Office of Scientific and Technical Information (OSTI), November 2017. http://dx.doi.org/10.2172/1410667.

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